CN103113481A - Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof - Google Patents
Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof Download PDFInfo
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Abstract
The invention provides a Bacillus subtilis mutant strain ZN0871v11 cell wall peptidoglycan which is prepared by disrupting Bacillus subtilis mutant strain ZN0871v11 cells of which the preservation number is CCTCC NO: M2011208 and removing the protein, teichoic acid and fat in the substance obtained from disruption. The mutant strain ZN0871v11 cell wall peptidoglycan comprises the following specific extraction process: 1. culturing the cells; 2. centrifuging; 3. disrupting the cell walls and diethyl ether extraction fat substances; and 4. carrying out protein proteolysis. The invention further provides an application of the Bacillus subtilis mutant strain ZN0871v11 cell wall peptidoglycan in rapidly and directly treatment of organic industrial wastewater of which the pH is 2.5-7.0 and the temperature is 4-80 DEG C. Compared with the prior art, the extracted mutant strain ZN0871v11 cell wall peptidoglycan can be used for directly, rapidly and efficiently treating organic wastewater, and both the treatable temperature and the treatable pH value range of the wastewater are greatly increased, and the energy consumption in wastewater treatment and the process procedure is shortened.
Description
Technical field
The present invention relates to a kind of mutant strain ZN0871v11 whole cell peptidoglycan, and provide its preparation method and in the purposes in the treatment of Organic Wastewater such as printing and dyeing field, belong to the treatment of Organic Wastewater such as printing and dyeing field.
Summary of the invention
The industry such as weaving, petrochemical industry, coking and fermentation are the important component parts of China's economy, to the development of national economy and the raising of living standards of the people, play a part very important., these industry have also produced a large amount of reluctant organic waste water when increasing GDP.And present waste water treatment process long operational time, the expense take active sludge as main body of utilizing is high, water outlet often be difficult to up to standard, when especially the water yield is large.Flocculence is good although the flocculation agent of biogenetic derivation has, effect stability, non-secondary pollution, the characteristic such as safe and harmless, can overcome the intrinsic defective of inorganic flocculating agent and Synthetical Organic Polymeric Flocculants itself, but most of existing biological flocculants such as starch based, polygalactomannan class, derivatived cellulose class, microbial polysaccharide class and complex biological coagulating agent etc. have weak point in the use, and it is low that COD removes efficient.Microbe-derived flocculation agent has non-secondary pollution and non-harmful good character, but except microbial cell, the yielding poorly of these microbial metabolites class flocculation agents, and cost is high, and is disposable.Therefore, the research and development novel waster water process that can fast processing especially contains the waste water such as printing and dyeing of hardly degraded organic substance has great importance to protection of the environment, recycle-water resource.
Summary of the invention
Subtilis is mutant strain ZN0871v11, that Bacillus subtilis ZN0871 bacterial strain is through ultraviolet mutagenesis, transformation mutant strain out, Classification And Nomenclature is subtilis Bacillus subtilis, be preserved in Chinese Typical Representative thing preservation center on June 23rd, 2011, be called for short CCTCC, its preserving number is CCTCC NO:M2011208.
This mutant strain has rapidly and efficiently flocculation and adsorption effect to organic pollutants such as sun blue, and its adsorption effect is compared with the wild-type subtilis and improved more than 5 times.Finding through further investigation, is that absorption and agglutination by cell causes to the absorption of dyestuff, and the active position of absorbing dye is positioned on the whole cell peptidoglycan of bacterium, and this process is very rapid, only needs 5～10 minutes.
The invention provides a kind of subtilis (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan that can be used as flocculation agent and sorbent material, by preserving number be the bacillus subtilis mutant strain ZN0871v11 cell of CCTCC NO:M2011208 after cytoclasis, broken gained material is removed protein, teichoic acid and lipid and is got.
The present invention also provides above-mentioned subtilis (Bacillus subtilis) the mutant strain ZN0871v11 extraction process of whole cell peptidoglycan, that the bacillus subtilis mutant strain ZN0871v11 that will cultivate is after cytoclasis, broken gained material removed protein, teichoic acid and lipid and obtain, specifically comprising the following steps:
(1), spawn culture: mutant strain ZN0871 v11 is inoculated into 1% inoculum size is cultured to activation in liquid nutrient medium, the mutant strain ZN0871 v11 cell after then activating is transferred to be cultivated in fresh liquid nutrient medium 30～36 hours;
(2), centrifugal: with cultivate in step (1) complete gained bacterium liquid at 10000～12000 rpm/min, under 4～10 ℃ of environment centrifugal 2～5 minutes, abandon the supernatant collecting precipitation and get precipitate A, after precipitate A adds the sterilized water washing, centrifugal collecting precipitation gets deposit B;
(3), fracturing cell walls: naturally cooling after adding after buffer A more than microwave heating to 80 ℃ in deposit B, then being placed in ice bath processes with the ultrasonic disruption instrument, until it is interior without complete cell, then at 12000～13000 rpm/min, under 4 ℃ of environment centrifugal 10 minutes, abandon the supernatant collecting precipitation, ratio in 0.1～1 g precipitation/10 mL ether adds anhydrous diethyl ether with the extracting lipid material in precipitation again, at room temperature place 30～60 min after stirring, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 4～10 ℃ of conditions, add 10 mL dehydrated alcohols by every 0.05～1 g precipitation capacity in the precipitation of collecting, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 4～10 ℃ of conditions, add 0.5～1 mL distilled water to make precipitation resuspended and centrifugal to wash out residual ethanol by every 0.05～1 g precipitation capacity in precipitation again, washing repeats more than 2 times, after last washing, centrifugal collecting precipitation gets deposit C,
(4), proteolysis: add buffer B that cell debris is precipitated in deposit C resuspended, remove DNA and RNA in cell, then by to add 10～15 μ L concentration in every ml soln be 10 mg/mL protein enzyme solutions and mix, insulation is more than 8 hours in being positioned over water bath with thermostatic control; Then at 12000～13000 rpm/min, abandon the supernatant collecting precipitation after centrifugal 8～10 minutes under 4 ℃ of conditions and must precipitate D, to precipitate D with damping fluid C washing 2～3 times after, adding concentration in D in precipitation again is 10% TCA solution, and with temperature regulation to 20～37 ℃ insulation 18～24 h, at last the liquid after resuspended is abandoned supernatant, collecting precipitation after under 12000～13000 rpm/min, 4 ℃ of conditions centrifugal 8～10 minutes, make bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan.
In step (1), liquid nutrient medium is liquid LB substratum.
In step (3), buffer A is 50 mM Tris-HCl(pH7.0), the time of microwave heating is 2～5 min, and the heating segmentation is carried out, and be 30 s every period heat-up time, and interval 10 s between section and section heating; The adding distil water washing step repeats 3 times.
In step (4), buffer B is 50 mM Tris-HCl(pH8.0～8.5), damping fluid C is 50 mM Tris-HCl(pH7.8～8.3); The described concrete grammar of DNA and RNA in cell of removing is for adding DNase and RNase degradation of dna and RNA in deposit C; Described proteolytic enzyme is protease P 16.
The present invention also provides the purposes of bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan simultaneously, and bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan is directly used in the organic industrial sewage of fast processing pH2.5～7.0 as flocculation agent and/or sorbent material.
And mutant strain ZN0871v11 whole cell peptidoglycan can be directly used in the organic industrial sewage under 4～80 ℃ of conditions of temperature is carried out fast processing.
Described mutant strain ZN0871v11 whole cell peptidoglycan is in metal cations Fe
2+Existence under organic industrial sewage is directly carried out fast processing.Process 100 mL organic industrial sewage metal cations Fe used
2+Amount be 15～20 mg.
The sewage that the mutant strain ZN0871v11 whole cell peptidoglycan that makes in the present invention is 250 mg/L to dye strength decolours fully only needs 5～10 minutes, in the rear sewage of decolouring, COD reduces to " 0 ", this process is far away higher than the report of other biological flocculation agent, and it to organic ratio of adsorption is: whole cell peptidoglycan: organism is 1:10～more than 12.5.
The present invention compared with prior art has following advantage: 1, available technology adopting mutant strain ZN0871v11 processes sewage as microbial flocculant, can cause the release of cell content in the time of in overbasic waste water in time is not adjusted to target pH scope and strengthens the difficulty that reduces COD.And in the present invention, the functional component whole cell peptidoglycan is extracted and go out directly the sewage effect, but therefore increased greatly the treatment temp of sewage and can process the pH value scope, thus the release conditions of cell content avoided, alleviated the pressure of aftertreatment.2, directly adopt whole cell peptidoglycan that industrial organic sewage is processed in the present invention, this effective constituent is directly carried out adsorption to the organic pollutant in sewage, therefore the required sorbent material consumption of sewage of processing same amount significantly reduces, so alleviated transportation cost, dwindled the user follow-up work treatment capacity and reduced requirement to equipment.3, in the present invention, the process range of mutant strain ZN0871v11 whole cell peptidoglycan is very wide, can carry out adsorption treatment for all organic industrial sewages, and as the organic waste water of the industries such as printing and dyeing, papermaking, petrochemical industry, treatment effect is good.
Embodiment
Below in conjunction with specific embodiment, the present invention is done detailed specific description.
Embodiment 1
Adopt following methods to prepare mutant strain ZN0871v11 whole cell peptidoglycan in the present embodiment: (1), mutant strain ZN0871 v11 is inoculated in liquid LB substratum with 1% inoculum size is cultured to activation, the mutant strain ZN0871 v11 cell after then activate were transferred in fresh liquid LB substratum cultivation 32 hours.
(2), with cultivate in step (1) complete gained bacterium liquid at 10000 rpm/min, under 5 ℃ of environment centrifugal 3 minutes, abandon the supernatant collecting precipitation, get precipitate A; Precipitate A adds the rear centrifugal collecting precipitation of sterilized water washing, gets deposit B;
(3), add 50 mM Tris-HCl(pH7.0 in deposit B) damping fluid, microwave heating 3 min, after cooling, deposit B be placed in ice bath and carry out ultrasonication with the ultrasonic disruption instrument, until microscopy is without complete cell, then at 12000 rpm/min, under 4 ℃ of environment centrifugal 10 minutes, abandon the supernatant collecting precipitation, then the ratio according to 0.2 g/10 mL adds anhydrous diethyl ether in precipitation, at room temperature place 35 min after stirring, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 5 ℃ of conditions, ratio according to 0.2 g precipitation/10 mL in the precipitation of collecting adds dehydrated alcohol, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 5 ℃ of conditions, ratio in every 0.2 g precipitation/1 mL adds distilled water to make precipitation resuspended and centrifugal to wash out residual ethanol in precipitation again, washing repeats 3 times, after last washing, centrifugal collecting precipitation gets deposit C,
(4), add 50 mM Tris-HCl(pH8.0 in deposit C), add DNA and RNA in DNase and RNase degraded deposit C, then after adding protease P 16 in the ratio that adds 10 μ L protein enzyme solutions in every ml soln, mix, being placed in 60 ℃ of waters bath with thermostatic control is incubated more than 8 hours, then 12000 rpm/min, abandon the supernatant collecting precipitation after centrifugal 8 minutes under 4 ℃ of conditions, must precipitate D; With 50 mM Tris-HCl(pH7.8) will precipitate D washing 2 times after, adding concentration in D in precipitation is 10% TCA solution, and with temperature regulation to 25 ℃ insulation 19 h, at last the liquid after resuspended is abandoned supernatant, collecting precipitation after under 12000 rpm/min, 4 ℃ of conditions centrifugal 8 minutes, make bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan.
The time of described microwave heating is 2～5 min, and the heating segmentation is carried out, and be 30 s every period heat-up time, and interval 10 s between section and section heating.
Bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan freeze-drying in freeze drier with making previously adds sterilized water resuspended, and adjusting whole cell peptidoglycan concentration is 10 mg/mL.getting 0.2 mL sun blue solution joins in the centrifuge tube of 1.5 mL, and add 5 μ L fluorescence dye To-Pro reagent, mixing afterreaction 30-60 min, add again 0.1 mL peptidoglycan suspension reaction 30-50 min in centrifuge tube, subsequently at 11600-12000 rpm/min, under 4 ℃ of conditions, centrifugal 7-10 min is to remove unconjugated dyestuff, collecting precipitation and to add 0.5-1 mL distilled water resuspended, at 11600-12000 rpm/min, under 4 ℃ of conditions, centrifugal 7-10 min is with the unconjugated dyestuff of wash residual, collecting precipitation, add 0.1 mL distilled water resuspended, get 10-15 μ L and do the laser confocal scanning detection.Through 642 nm exciting light scanning discoveries, under 661 nm emission wavelengths, difform solid particulate has strong fluorescence.This shows: cell eliminates inclusion through discharging after abundant broken wall, extracts lipid material, except the peptidoglycan layer of the solid form of deproteinize and teichoic acid be the active position of absorption and combination dye.
The bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan that now makes carries out the sewage disposal experiment as flocculation agent or sorbent material:
First with 250 mg sun blue dyestuffs, 200 mg Fe
2+Be dissolved in 1000 mL distilled water, transfer pH5.0, get respectively 50～250 μ L peptidoglycan suspensions in the ratio of arithmetic progression and respectively join in 100 mL dye solution solution, take the corresponding dye solution that do not add peptidoglycan as contrast, place 5～10 min after mixing.Subsequently, take the corresponding dye solution that do not add peptidoglycan as reference, get the centrifuged supernatant photometry absorption value of 5 mL liquid, and survey COD, calculate adsorption efficiency.The wavelength of surveying sun blue solution absorbance value is 545 nm.Result: be 30 ℃, metal cations Fe in pH5.0, temperature
2+Final concentration is under 20 mg/100 mL conditions, and the percent of decolourization of mutant strain ZN0871v11 whole cell peptidoglycan in 5～10 min reached 100%, and colourity is reduced to " 0 ", and COD reduces to " 0 ", and adsorption efficiency is 1:10～12.5.
The percent of decolourization of dyestuff=(control group OD value-experimental group OD value)/control group OD * 100%
Be 37 ℃, metal cations Fe in pH5.0, temperature
2+Final concentration is under 20 mg/100 mL conditions, adds mutant strain ZN0871v11 whole cell peptidoglycan mixing, places 5～10 minutes, and mutant strain ZN0871v11 whole cell peptidoglycan is 100% to the flocculation efficiency of sun blue, records COD and reduces to " 0 ".
At pH7.0, temperature is 25 ℃, Fe
2+Concentration be respectively under the condition of 15,20 mg/100 mL, mutant strain ZN0871v11 peptidoglycan is complete to the decolouring of sun blue dyestuff, in 5～10 minutes, reaches maximum elimination factor, is 100% to the flocculating rate of sun blue dyestuff, and records COD and reduce to " 0 ".
In the present embodiment in metal cations Fe
2+Final concentration is under 15～20 mg/100 mL conditions, tested respectively pH2.5～9.0, temperature and is in the scope of 4～80 ℃, adds in proportion after mutant strain ZN0871v11 whole cell peptidoglycan the flocculating effect to dyestuff.Experimental result is as follows: place after mixing in 5～10 minutes, in 4～80 ℃, pH2.5～7.0 scopes, mutant strain ZN0871v11 whole cell peptidoglycan is 100% to the adsorption efficiency of dyestuff, records COD and reduces to " 0 ".
Embodiment 2
Adopt following methods to prepare mutant strain ZN0871v11 whole cell peptidoglycan in the present embodiment: (1), mutant strain ZN0871 v11 is inoculated in liquid LB substratum with 1% inoculum size is cultured to activation, the mutant strain ZN0871 v11 cell after then activate were transferred in fresh liquid LB substratum cultivation 35 hours;
(2), with cultivate in step (1) complete gained bacterium liquid at 11500 rpm/min, under 8 ℃ of environment centrifugal 4 minutes, abandon the supernatant collecting precipitation, get precipitate A; Precipitate A adds the rear centrifugal collecting precipitation of sterilized water washing, gets deposit B;
(3), add 50 mM Tris-HCl(pH7.0 in deposit B) damping fluid, microwave heating 4 min, after cooling, deposit B is placed in ice bath, and carry out ultrasonication with the ultrasonic disruption instrument, until microscopy is without complete cell, then at 12500 rpm/min, under 4 ℃ of environment centrifugal 10 minutes, abandon the supernatant collecting precipitation, then the ratio according to 0.7 g/10 mL adds anhydrous diethyl ether in precipitation, at room temperature place 55 min after stirring, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 8 ℃ of conditions, ratio according to 0.6g precipitation/10 mL in the precipitation of collecting adds dehydrated alcohol, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 8 ℃ of conditions, ratio in every 0.6 g precipitation/1 mL adds distilled water to make precipitation resuspended and centrifugal to wash out residual ethanol in precipitation again, washing repeats 3 times, centrifugal collecting precipitation after last washing, get deposit C,
(4), add 50 mM Tris-HCl(pH8.4 in deposit C), add DNA and RNA in DNase and RNase degraded deposit C, then mix after adding protease P 16 in the ratio that adds 15 μ L protein enzyme solutions in every ml soln, being placed in 60 ℃ of waters bath with thermostatic control is incubated more than 8 hours, then at 12500 rpm/min, abandon the supernatant collecting precipitation after centrifugal 9.5 minutes under 4 ℃ of conditions, must precipitate D; With 50 mM Tris-HCl(pH8.2) will precipitate D washing 2～3 times after, adding concentration in D in precipitation is 10% TCA solution and with temperature regulation to 35 ℃ insulation 18 h, at last the liquid after resuspended is abandoned supernatant, collecting precipitation after under 12500 rpm/min, 4 ℃ of conditions centrifugal 9.5 minutes, make bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan.
The time of described microwave heating is 2～5 min, and the heating segmentation is carried out, and be 30 s every period heat-up time, and interval 10 s between section and section heating.
The bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan that makes in the present embodiment is carried out the sewage disposal experiment, and experimentation and result are as follows:
In metal cations Fe
2+Final concentration be under 15～20 mg/100 mL conditions, tested respectively the experiment that mutant strain ZN0871v11 whole cell peptidoglycan is processed the water sample of the former water in the former water of printing and dyeing enterprise, paper mill and petrochemical complex.Add respectively mixing after 29.1 mg peptidoglycans in every liter of waste water, flocculation/absorption 5～10 minutes, the result of sampling and testing is, at room temperature, pH3.5～5.5 o'clock, mutant strain ZN0871v11 whole cell peptidoglycan is 141.1 times to the adsorption efficiency of the former water of printing and dyeing enterprise, the elimination factor of COD is 99.39%, records colourity and reduces to " 0 ", and COD reduces to 25 mg/L by 4130 mg/L; The former colority of water in paper mill is reduced to " 0 ", and the elimination factor of COD is that 97.55%, COD reduces to 200 mg/L by 8150 mg/L; Adsorption efficiency to 2 water samples of petrochemical complex is respectively 21.036 times, 8.053 times, record colourity and reduce to " 0 ", the elimination factor of COD is respectively 95.88% and 97.57%, its COD respectively by 6380 mg/L reduce to 262.7 mg/L, 2400 mg/L reduce to 58.3 mg/L.
Claims (9)
1. subtilis (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan, by the preserving number of cultivating be the bacillus subtilis mutant strain ZN0871v11 of CCTCC NO:M2011208 after cytoclasis, broken gained material is removed protein, teichoic acid and lipid and is got.
2. the extraction process of subtilis claimed in claim 1 (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan, the bacillus subtilis mutant strain ZN0871v11 that it is characterized in that cultivating is after cytoclasis, broken gained material removed protein, teichoic acid and lipid and obtain, specifically comprising the following steps:
(1), spawn culture: mutant strain ZN0871 v11 is inoculated in liquid nutrient medium with 1% inoculum size activates, the mutant strain ZN0871 v11 cell after then activating is transferred to be cultivated in fresh liquid nutrient medium 30～36 hours;
(2), centrifugal: with cultivate in step (1) complete gained bacterium liquid at 10000～12000 rpm/min, under 4～10 ℃ of environment centrifugal 2～5 minutes, abandon the supernatant collecting precipitation and get precipitate A, after precipitate A adds the sterilized water washing, centrifugal collecting precipitation gets deposit B;
(3), fracturing cell walls: naturally cooling after adding after buffer A more than microwave heating to 80 ℃ in deposit B, then being placed in ice bath processes with the ultrasonic disruption instrument, until it is interior without complete cell, then at 12000～13000 rpm/min, under 4 ℃ of environment centrifugal 10 minutes, abandon the supernatant collecting precipitation, ratio in 0.1～1 g precipitation/10 mL ether adds anhydrous diethyl ether with the extracting lipid material in precipitation again, at room temperature place 30～60 min after stirring, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 4～10 ℃ of conditions, add 10 mL dehydrated alcohols by every 0.05～1 g precipitation capacity in the precipitation of collecting, then at 12000 rpm/min, collecting precipitation after centrifugal 10 minutes under 4～10 ℃ of conditions, add 0.5～1 mL distilled water to make precipitation resuspended and centrifugal to wash out residual ethanol by every 0.05～1 g precipitation capacity in precipitation again, washing repeats more than 2 times, after last washing, centrifugal collecting precipitation gets deposit C,
(4), proteolysis: add buffer B that cell debris is precipitated in deposit C resuspended, remove DNA and RNA in cell, then by to add 10～15 μ L concentration in every ml soln be 10 mg/mL protein enzyme solutions and mix, then be positioned in water bath with thermostatic control insulation more than 8 hours; Then at 12000～13000 rpm/min, abandon the supernatant collecting precipitation after centrifugal 8～10 minutes under 4 ℃ of conditions, must precipitate D; To precipitate D with damping fluid C washing 2～3 times after, adding concentration in D in precipitation again is 10% TCA solution, and with temperature regulation to 20～37 ℃ insulation 18～24 h, at last the liquid after resuspended is abandoned supernatant, collecting precipitation after under 12000～13000 rpm/min, 4 ℃ of conditions centrifugal 8～10 minutes, make bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan.
3. the extraction process of subtilis according to claim 2 (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan is characterized in that: in step (1), liquid nutrient medium is liquid LB substratum.
4. the extraction process of subtilis according to claim 2 (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan, it is characterized in that: in step (3), buffer A is 50 mM Tris-HCl, pH7.0, the time of microwave heating is 2～5 min, the heating segmentation is carried out, be 30 s every period heat-up time, and interval 10 s between section and section heating; The adding distil water washing step repeats 3 times.
5. the extraction process of subtilis according to claim 2 (Bacillus subtilis) mutant strain ZN0871v11 whole cell peptidoglycan, it is characterized in that: in step (4), buffer B is 50 mM Tris-HCl, pH8.0～8.5, damping fluid C is 50 mM Tris-HCl, pH7.8～8.3; The described concrete grammar of DNA and RNA in cell of removing is for adding DNase and RNase degradation of dna and RNA in deposit C; Described proteolytic enzyme is protease P 16.
6. the purposes of bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan claimed in claim 1, is characterized in that: bacillus subtilis mutant strain ZN0871v11 whole cell peptidoglycan is directly used in the organic industrial sewage of fast processing pH2.5～7.0 as flocculation agent and/or sorbent material.
7. the purposes of mutant strain ZN0871v11 whole cell peptidoglycan according to claim 6, it is characterized in that: described mutant strain ZN0871v11 whole cell peptidoglycan is in metal cations Fe
2+Existence under organic industrial sewage is directly carried out fast processing.
8. the purposes of mutant strain ZN0871v11 whole cell peptidoglycan according to claim 7, is characterized in that: process 100 mL organic industrial sewage metal cations Fe used
2+Amount be 15～20 mg.
9. the purposes of mutant strain ZN0871v11 whole cell peptidoglycan according to claim 6, it is characterized in that: mutant strain ZN0871v11 whole cell peptidoglycan directly carries out fast processing to the organic industrial sewage under 4～80 ℃ of conditions of temperature.
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| CN102321187A (en) * | 2011-08-15 | 2012-01-18 | 内蒙古双奇药业股份有限公司 | Method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501 |
| CN102352325A (en) * | 2011-06-27 | 2012-02-15 | 中南民族大学 | Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water |
| CN102617702A (en) * | 2012-03-09 | 2012-08-01 | 姜浩奎 | Production method of peptidoglycan |
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| CN102321187A (en) * | 2011-08-15 | 2012-01-18 | 内蒙古双奇药业股份有限公司 | Method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501 |
| CN102617702A (en) * | 2012-03-09 | 2012-08-01 | 姜浩奎 | Production method of peptidoglycan |
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