CN103103226A - Method for preparing soybean phospholipid with low iodine value - Google Patents
Method for preparing soybean phospholipid with low iodine value Download PDFInfo
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- CN103103226A CN103103226A CN 201210451304 CN201210451304A CN103103226A CN 103103226 A CN103103226 A CN 103103226A CN 201210451304 CN201210451304 CN 201210451304 CN 201210451304 A CN201210451304 A CN 201210451304A CN 103103226 A CN103103226 A CN 103103226A
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- soybean phospholipid
- iodine number
- low iodine
- phosphatide
- palmitinic acid
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Abstract
The invention discloses a method for preparing soybean phospholipid with low iodine value. The method comprises the following steps of: dissolving soybean phospholipid into n-hexane, adding palmitic acid, adding a certain quantity of immobilized lipase Lipozyme TLIM, reacting for a certain period of time at a certain temperature, filtering, removing the solvent, washing with acetone till free fatty acid does not exist, and thus obtaining the soybean phospholipid with low iodine value, wherein the iodine value (gI2/ 100g) of the soybean phospholipid is reduced from 96 to 69.
Description
Technical field
A kind of method of low iodine number soybean phospholipid preparation belongs to grease processing technology field.
Background technology
Soybean phospholipid is a kind of natural surface active agent, the functions such as emulsification, dispersion, foaming, demoulding, reduction viscosity, maintenance moisture are arranged, but it contains more unsaturated double-bond in forming, unstable, easily oxidation in air, darken, produce the volatile small-molecule substances such as aldehyde ketone, this has limited its application to a great extent.For overcoming above shortcoming, the method for industrial hydrogenation commonly used reduces its iodine number.But easily produce trans fatty acid in hydrogenation process, human body is difficult to digest this type of unsaturated fatty acids, after it is eaten, mostly is stranded in human body, and then increases the probability of suffering from cardiovascular disease.Studies show that, can the raise content of low-density lipoprotein (LDL) of trans fatty acid reduces the content (HDL) of high-density lipoprotein (HDL), and therefore the saturation ratio that improves phosphatide of the way by hydrogenation can not adapt to modern health requirements.
Summary of the invention
The object of the present invention is to provide a kind of method of low iodine number soybean phospholipid preparation.Soybean phospholipid is dissolved in normal hexane, adds palmitinic acid, adds a certain amount of immobilized lipase Lipozyme TLIM, reacts at a certain temperature certain hour, filter, and precipitation, washing with acetone obtains low iodine number soybean phospholipid to without free fatty acids.
Technical scheme of the present invention: a kind of method of low iodine number soybean phospholipid preparation is: soybean phospholipid is dissolved in normal hexane, add palmitinic acid, add a certain amount of immobilized lipase Lipozyme TLIM, react at a certain temperature certain hour, filter, precipitation, washing with acetone obtains low iodine number soybean phospholipid to without free fatty acids.
(1) raw material: soybean phospholipid, acetone insoluble matter are greater than 98%, and iodine number is 96 (gI
2/ 100g), palmitinic acid purity is greater than 95%;
(2) mass ratio of palmitinic acid and phosphatide is 1.84-1.72: 1;
(3) mass volume ratio 0.21 (g/mL) of phosphatide and normal hexane;
(4) enzyme that uses is the 15%-21% of phosphatide and palmitinic acid total amount as immobilized lipase Lipozyme TLIM, enzyme concentration;
(5) temperature of reaction is 55 ℃-62 ℃;
(6) reaction times 35h-48h.
Beneficial effect of the present invention: a kind of method of low iodine number soybean phospholipid preparation, provide a kind of feasible method for preparing low iodine number soybean phospholipid, use the method can prepare easily the low iodine number soybean phospholipid product that has extensive use in foodstuffs industry.
Table 1: the method products obtained therefrom index
Embodiment
Embodiment 1
100 gram soybean phospholipids are dissolved in the 500mL normal hexane, add 172 gram palmitinic acids, stirring and dissolving, add 40.8 gram Lipozyme TLIM, in 55 ℃ of stirring reaction 48h, filter to remove Lipozyme TLIM, the filtrate precipitation, obtaining iodine number with the 1000mL washing with acetone is 68 low iodine number soybean phospholipid 92 grams.
Embodiment 2
100 gram soybean phospholipids are dissolved in the 500mL normal hexane, add 184 gram palmitinic acids, stirring and dissolving, add 59.6 gram Lipozyme TLIM, in 62 ℃ of stirring reaction 35h, filter to remove Lipozyme TLIM, the filtrate precipitation, obtaining iodine number with the 1000mL washing with acetone is 69 low iodine number soybean phospholipid 89 grams.
Embodiment 3
100 gram soybean phospholipids are dissolved in the 500mL normal hexane, add 184 gram palmitinic acids, stirring and dissolving, add 59.6 gram Lipozyme TLIM, in 55 ℃ of stirring reaction 48h, filter to remove Lipozyme TLIM, the filtrate precipitation, obtaining iodine number with the 1000mL washing with acetone is 67 low iodine number soybean phospholipid 91 grams.
Claims (4)
1. the method for one kind low iodine number soybean phospholipid preparation is: soybean phospholipid is dissolved in normal hexane, add palmitinic acid, add a certain amount of immobilized lipase Lipozyme TLIM, react at a certain temperature certain hour, filter, precipitation, washing with acetone obtains low iodine number soybean phospholipid to without free fatty acids.
(1) raw material: soybean phospholipid, acetone insoluble matter be greater than 98%, iodine number (gI
2/ 100g) be 96, palmitinic acid purity is greater than 95%;
(2) mass ratio of palmitinic acid and phosphatide is 1.84-1.72: 1;
(3) mass volume ratio 0.21 (g/mL) of phosphatide and normal hexane;
(4) enzyme that uses is the 15%-21% of phosphatide and palmitinic acid total amount as immobilized lipase Lipozyme TLIM, enzyme concentration;
(5) temperature of reaction is 55 ℃-62 ℃;
(6) reaction times 35h-48h.
2. the method for a kind of low iodine number soybean phospholipid preparation according to claim 1, the mass ratio that it is characterized in that palmitinic acid and phosphatide is 1.84-1.72: 1.
3. the method for a kind of low iodine number soybean phospholipid preparation according to claim 1, is characterized in that the enzyme that uses is immobilized lipase Lipozyme TLIM, and enzyme concentration is the 15%-21% of phosphatide and palmitinic acid total amount.
4. the method for a kind of low iodine number soybean phospholipid preparation according to claim 1, is characterized in that 55 ℃-62 ℃ of temperature of reaction, reaction times 35h-48h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210451304 CN103103226A (en) | 2012-11-10 | 2012-11-10 | Method for preparing soybean phospholipid with low iodine value |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210451304 CN103103226A (en) | 2012-11-10 | 2012-11-10 | Method for preparing soybean phospholipid with low iodine value |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103103226A true CN103103226A (en) | 2013-05-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210451304 Pending CN103103226A (en) | 2012-11-10 | 2012-11-10 | Method for preparing soybean phospholipid with low iodine value |
Country Status (1)
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| CN (1) | CN103103226A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107788525A (en) * | 2017-10-30 | 2018-03-13 | 广州润虹医药科技股份有限公司 | A kind of toxin-expelling and face nourishing nutrient powder and preparation method thereof |
| CN108795998A (en) * | 2018-07-04 | 2018-11-13 | 嘉必优生物技术（武汉）股份有限公司 | A method of reducing glyceride iodine number |
-
2012
- 2012-11-10 CN CN 201210451304 patent/CN103103226A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107788525A (en) * | 2017-10-30 | 2018-03-13 | 广州润虹医药科技股份有限公司 | A kind of toxin-expelling and face nourishing nutrient powder and preparation method thereof |
| CN108795998A (en) * | 2018-07-04 | 2018-11-13 | 嘉必优生物技术（武汉）股份有限公司 | A method of reducing glyceride iodine number |
| CN108795998B (en) * | 2018-07-04 | 2021-11-19 | 嘉必优生物技术（武汉）股份有限公司 | Method for reducing iodine value of glyceride |
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| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130515 |