CN103091488A - Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay - Google Patents
Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay Download PDFInfo
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- CN103091488A CN103091488A CN2013100110948A CN201310011094A CN103091488A CN 103091488 A CN103091488 A CN 103091488A CN 2013100110948 A CN2013100110948 A CN 2013100110948A CN 201310011094 A CN201310011094 A CN 201310011094A CN 103091488 A CN103091488 A CN 103091488A
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Abstract
The invention relates to a sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay. The sample application plate comprises a substrate for connecting a fixed platform frame of a sample application instrument with a plurality of positioning columns arranged on the substrate, wherein a plurality of positioning intervals for positioning and holding the membrane strips to be coated are formed among the plurality of positioning columns. According to the invention, by means of stably setting the membrane strips to be coated in the sample application intervals through the sample application plate with a plurality of sample application intervals formed through the plurality of positioning columns, coating equipment can complete the sample application of a plurality of membrane strips to be coated once, so that the coating efficiency is improved, a large amount of operation time is saved, and the labor intensity is reduced.
Description
Technical field
the invention belongs to diagnosis immuno analytical method field, particularly relate to the point template for the treatment of the coated film bar in a kind of film class diagnosis immunoassay for placement.
Background technology
film class diagnostic reagent product generally includes two kinds, and a kind of is lateral chromatography class diagnostic reagent product; Another kind is strip analysis method diagnostic reagent product.
the lateral chromatography technology is that antigen or antibody are coated in to solid phase carrier (normally nitrocellulose filter) through drawing film instrument (point sample instrument) with the band dress, collaurum (or latex particle) labelled reagent (antibody or monoclonal antibody) is adsorbed on pad, after sample to be checked is added on the sample pad of test strips one end, by capillary action, move forward, after dissolving the colloid gold label reagent on pad, react to each other, while moving to again fixing antigen or antibody regional, specific binding occurs with it again and is trapped in the bond of thing to be checked and gold marked reagent, be gathered in to detect and be with, can be by being observed visually the colour developing result.This method has now developed into diagnosis test paper, uses very convenient.
it is that one or more antigens are filled to parallel being printed on nitrocellulose membrane by drawing film instrument (point sample instrument) with band that the band enzyme is exempted from the analytic approach technology, after sealer is processed, and the non-specific site on the sealing tunica fibrosa, thereby the generation of prevention nonspecific reaction.During detection, the film bar is combined the antigen of antibody on the film bar in serum with the common incubation of the serum sample of dilution.Utilize the anti-human IgG of enzyme labeling to detect this binding antibody.After adding substrate, the substrate for enzymatic activity of mark reaction on binding antibody, and produce visible band at antigen coated position (detection line).Whether develop the color according to band, can judge experimenter's antibody characteristic, if obviously colour developing is judged to be this antibody positive, if do not develop the color be judged to be feminine gender.
when coated, by a nitrocellulose filter cut, (normal length is 26-30cm, width is 0.5-2cm) be placed on coated equipment (normally drawing film instrument or point sample instrument) ad-hoc location (fixing sample application platform, for only at upper and lower two ends, setting the stationary platform of bit field, non-dismountable) on, coated equipment is drawn coating buffer or be sprayed onto on nitrocellulose filter, after coated the end, takes off the film bar.Every stroke/spray a film (approximately 100 person-portion detection limits) need to place the film bar, line, take off 3 steps of film bar, also needs location while placing the film bar.
as can be seen here, above-mentioned method for coating, obviously still have inconvenience and defect, and labour intensity is large, and efficiency is very low, is badly in need of further improving.How to found a kind of coated higher method of efficiency, one of real important subject that belongs to current this area of making.
Summary of the invention
technical matters to be solved by this invention is to overcome the deficiencies in the prior art, and the point template for the treatment of the coated film bar in a kind of film class diagnosis immunoassay for placement is provided, and takes this point template can realize higher coated efficiency.
for solving above technical matters, the present invention takes following technical scheme:
treat the point template of coated film bar in a kind of film class diagnosis immunoassay for placement, it comprises substrate for connecting point sample instrument stationary platform frame and is arranged on a plurality of positioning rails on substrate, between described a plurality of positioning rails, forms a plurality ofly for locating to place, to treat between the positioning area of coated film bar.
preferably, substrate can be arranged on the stationary platform frame of point sample instrument with dismantling.
preferably, a plurality of positioning rails parallel setting, between adjacent two positioning rails, form between a positioning area.
further, the square framework that the stationary platform frame of point sample instrument consists of four positioning strips, and can link together between four positioning strips with dismantling.
more preferably, substrate is square, and the bearing of trend of a plurality of positioning rails is parallel to the length of side bearing of trend of substrate.
the concrete enforcement according to the present invention, the point sample interval has 10～30, and the disposable point sample of point sample instrument is 1000～3000 person-portions, is 10～30 times of prior art.
due to the enforcement of above technical scheme, the present invention compared with prior art has following advantage:
the present invention adopts the point template in a plurality of point samples interval formed by a plurality of positioning rails, to treat that the coated film bar stably is arranged in the point sample interval, and make coated equipment once can complete a plurality of point samples for the treatment of the coated film bar, improve coated efficiency, save a large amount of running times, reduce labour intensity.
The accompanying drawing explanation
below in conjunction with accompanying drawing and concrete embodiment, the present invention will be further described in detail:
fig. 1 is the structural representation according to point template of the present invention;
fig. 2 be point template shown in Fig. 1 and point sample instrument stationary platform frame in conjunction with schematic diagram;
wherein: 1, point template; 10, substrate; 11, positioning rail; 12, point sample interval; 2, stationary platform frame; 20a, 20b, 20c, 20d, positioning strip; 3, treat the coated film bar.
Embodiment
as depicted in figs. 1 and 2, treat the point template of coated film bar in the film class diagnosis immunoassay that the present embodiment provides for placement, it comprises substrate for connecting point sample instrument stationary platform frame 2 10 and is arranged on a plurality of positioning rails 11 on substrate 10, wherein between a plurality of positioning rails 11, forms a plurality ofly for locating, to place between the positioning area for the treatment of coated film bar 3 12.
be arranged on point sample instrument stationary platform frame 2 to substrate 10 dismountings of point template 1, be convenient to 10 replacings of substrate.Wherein substrate 10 is square, the bearing of trend of a plurality of positioning rails 11 is parallel to the length of side bearing of trend of substrate 10, a plurality of positioning rails 11 parallel setting, form between a positioning area 12 between adjacent two positioning rails 11, realize the location for the treatment of coated film bar 3 by positioning rail 11, the standing time of coated film bar 3 is treated in saving, improves installation effectiveness.
in this example, the stationary platform frame 2 of point sample instrument is removably, point sample interval 12 has 10～30, that is to say, point template once can be installed 10～30 and treat coated film bar 3, this point template 1 is placed on point sample instrument stationary platform frame 2 and is coated with, and the point template 1 that adopts same structure in a plurality of and this enforcement is as standby, when first point template 1 complete coated after, adopt second point template 1 to be changed, making the coated each point sample of equipment in 1000～3000 person-portions, is 10～30 times of prior art.
in the present embodiment, the stationary platform frame 2 of point sample instrument is four positioning strip 20a, 20b, 20c, the square framework that 20d forms, and can link together between four positioning strips 20 with dismantling, take positioning strip 20a and the positioning strip 20b of stationary platform frame 2 apart, conflict on positioning strip 20c and positioning strip 20d in two limits that square point template 1 is adjacent, positioning strip 20a and positioning strip 20b are restored to the original state simultaneously, then open point sample instrument, treating line on coated film bar 3, by A to B, again by C to D, always back and forth until the line of the coated film bar 3 that remains on completing substrate 10, close point sample instrument, again take positioning strip 20a and the positioning strip 20b of stationary platform frame 2 apart, conflict on positioning strip 20c and positioning strip 20d in two limits that point template 1 in standby is adjacent, the location that utilizes positioning strip 20c and positioning strip 20d can realize point template 1, then open point sample instrument, so repeatedly carry out.
the present invention adopts the point template in a plurality of point samples interval consisted of a plurality of positioning rails, to treat that the coated film bar is arranged in the point sample interval, make coated equipment once can complete a plurality of point samples for the treatment of the coated film bar, improve coated efficiency, according to the substrate of point template, can be arranged on point sample instrument stationary platform frame simultaneously with dismantling, conveniently treat the replacing of coated film bar, without the coated film bar for the treatment of that will be coated with after once completing, pull down, and the coated coated film bar for the treatment of is installed, save a large amount of running times, reduce labour intensity.
above the present invention is described in detail; its purpose is to allow the personage who is familiar with this art can understand content of the present invention and be implemented; can not limit the scope of the invention with this; the equivalence that all Spirit Essences according to the present invention are done changes or modifies, and all should be encompassed in protection scope of the present invention.
Claims (6)
1. a film class is diagnosed the point template for the treatment of the coated film bar in immunoassay for placement, it is characterized in that: described point template (1) comprises the substrate (10) for connecting point sample instrument stationary platform frame (2) and is arranged on a plurality of positioning rails (11) on described substrate (10), between described a plurality of positioning rails (11), forms a plurality ofly for locating, to place between the positioning area for the treatment of coated film bar (3) (12).
2. according to treat the point template of coated film bar in claim 1 film class diagnosis immunoassay for placement, it is characterized in that: described substrate (10) can be arranged on the stationary platform frame (2) of described point sample instrument with dismantling.
3. according to treat the point template of coated film bar in claim 1 film class diagnosis immunoassay for placement, it is characterized in that: described a plurality of positioning rails (11) parallel setting, between adjacent two described positioning rails (11), form between a described positioning area (12).
4. according to treat the point template of coated film bar in claim 1 film class diagnosis immunoassay for placement, it is characterized in that: the stationary platform frame (2) of described point sample instrument is four positioning strip (20a, 20b, 20c, square framework 20d) formed, and described four positioning strips (20a, 20b, 20c, 20d) between can link together with dismantling.
5. according to treat the point template of coated film bar in claim 1 or 3 or 4 described film class diagnosis immunoassays for placement, it is characterized in that: described substrate (10) is square, and the bearing of trend of described a plurality of positioning rails (11) is parallel to the length of side bearing of trend of described substrate (10).
6. treat the point template of coated film bar in film class diagnosis immunoassay according to claim 1 for placement, it is characterized in that: described point sample interval (12) has 10～30.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100110948A CN103091488A (en) | 2013-01-11 | 2013-01-11 | Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100110948A CN103091488A (en) | 2013-01-11 | 2013-01-11 | Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103091488A true CN103091488A (en) | 2013-05-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100110948A Pending CN103091488A (en) | 2013-01-11 | 2013-01-11 | Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2577283Y (en) * | 2002-11-08 | 2003-10-01 | 穆海东 | Protein chip |
| CN201096780Y (en) * | 2007-06-26 | 2008-08-06 | 上海裕隆生物科技有限公司 | A biologic chip sampling platform |
| CN102539740A (en) * | 2010-12-29 | 2012-07-04 | 河北省健海生物芯片技术有限责任公司 | Protein chip and method for detecting 80 kinds of autoantibodies simultaneously |
| CN102621302A (en) * | 2012-03-22 | 2012-08-01 | 四川省新成生物科技有限责任公司 | Linear immune detection film bar and manufacturing process thereof |
| CN202512117U (en) * | 2012-04-09 | 2012-10-31 | 无锡国盛精密模具有限公司 | Self-locking type slide mounting plate for biochip microarrayer |
| CN203011921U (en) * | 2013-01-11 | 2013-06-19 | 苏州浩欧博生物医药有限公司 | Point template for accommodating tunica strips to be packed in membrane type diagnosis immunoassay |
-
2013
- 2013-01-11 CN CN2013100110948A patent/CN103091488A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2577283Y (en) * | 2002-11-08 | 2003-10-01 | 穆海东 | Protein chip |
| CN201096780Y (en) * | 2007-06-26 | 2008-08-06 | 上海裕隆生物科技有限公司 | A biologic chip sampling platform |
| CN102539740A (en) * | 2010-12-29 | 2012-07-04 | 河北省健海生物芯片技术有限责任公司 | Protein chip and method for detecting 80 kinds of autoantibodies simultaneously |
| CN102621302A (en) * | 2012-03-22 | 2012-08-01 | 四川省新成生物科技有限责任公司 | Linear immune detection film bar and manufacturing process thereof |
| CN202512117U (en) * | 2012-04-09 | 2012-10-31 | 无锡国盛精密模具有限公司 | Self-locking type slide mounting plate for biochip microarrayer |
| CN203011921U (en) * | 2013-01-11 | 2013-06-19 | 苏州浩欧博生物医药有限公司 | Point template for accommodating tunica strips to be packed in membrane type diagnosis immunoassay |
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Application publication date: 20130508 |