CN103070951B - Externally used medicinal composition for treating cattle mange and preparation method thereof - Google Patents

Externally used medicinal composition for treating cattle mange and preparation method thereof Download PDF

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CN103070951B
CN103070951B CN201310040251.8A CN201310040251A CN103070951B CN 103070951 B CN103070951 B CN 103070951B CN 201310040251 A CN201310040251 A CN 201310040251A CN 103070951 B CN103070951 B CN 103070951B
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cattle
medicinal composition
mange
cortex
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CN103070951A (en
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赵�权
张媛
姜晶
刘靓
温伟
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Jilin Agricultural University
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Jilin Agricultural University
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Abstract

The invention discloses an externally used medicinal composition for treating cattle mange and a preparation method thereof. The externally used medicinal composition comprises the following medicinal herbs in parts by weight: 30-50 parts of nidus vespae, 20-40 parts of chaulmoogratree seed, 20-40 parts of viper's bugloss, 20-40 parts of golden larch bark, 20-40 parts of ulmus macrocarpa hance, 10-30 parts of sulphur, 20-40 parts of cortex dictamni, 20-40 parts of belvedere fruit, 20-40 parts of garter snake, 20-40 parts of pomegranate bark, 10-30 parts of alum, and 140-200 parts of mature vinegar, is prepared by organically combining the medicinal herbs in terms of dialectical compatibility and mutual assistance, and is enhanced in the effects of clearing heat, dispelling wind, removing mange and relieving itching, thereby achieving the purpose of curing the cattle mange. According to the externally used medicinal composition for treating the cattle mange and the preparation method thereof, the prepared externally used medicinal composition for treating the cattle mange is low in cost, easy to prepare, free from toxic or side effect and obvious in curative effect. Clinical practices prove that the externally used medicinal composition for treating the cattle mange has the cure rate of 100% and the effective rate of 100%.

Description

A kind of externally-applied medicinal composition for the treatment of cattle acaricide and preparation method thereof
technical field:
The present invention relates to externally-applied medicinal composition for the treatment of animal acaricide and preparation method thereof, belong to animal field of medicaments, particularly a kind of externally-applied medicinal composition that is specifically designed to treatment cattle acaricide and preparation method thereof.
background technology:
The acaricide of cattle is a kind of common epizootic disease being caused by acarid, is sporadic, endemicity are popular, and the play of take is itched and eczema-like dermatitis is feature.To be caused by Sarcoptes bovis, psoroptes bovis, cattle foot demodicid mite and cattle vermiform mite, and in the majority with the above two.Larger to cattle-raising harm.
1, pathogen life cycle and epidemiology:
Sarcoptes bovis: polypide digs cutter tunnel at epidermis, and grow therein breeding, female worm lay eggs in tunnel (produces 40-50 piece), the larva of hatching climbs to skin surface, and between hair, skin digs cutter vesicle, and degenerates into nymph inside, nymph pierces the shallow cave of skin, degenerates into adult inside.Average 15 days of acaricide growth course.
Cattle itches and walks haltingly: multiparasitization, at the skin surface of neck, shoulder and root of the tail, is not holed.Carcass is thin and weak, easy infection when resistance is poor.
At the beginning of primary disease mainly betides winter and last month of spring in autumn.Especially carcass sanitary condition is bad, under the higher condition of skin surface humidity, primary disease more easily occurs.Contagion is main mode of transmission, as drinking-water, administrative furnitures, harness all can infect.
2, symptom and pathological change: Sarcoptes bovis disease is to be caused by Sarcoptes bovis, multiple head, the cervical region be born in, also betides other positions during severe infections.There is at first brief summary, continue as vesicle, pathological changes portion pruritus, when nocturnal temperature is high, pruritus exacerbates.Because often biting and rub, skin injury is broken, flow out lymph fluid, the surface horny formation crust that comes off, moistening under crust, frowziness while having purulent secretion.Pars affecta skin is baldness frilling gradually, especially cervical region.Day by day become thin, when serious, cause death.
Psoroptes bovis disease is to be caused by psoroptes bovis, and primary disease starts from spreading when serious whole body.Pathological changes portion pruritus, tongue amputation is not licked and is rubbed the stake of tree sassafras, and transudate forms crust, and pachyderma, follows the string, and sky alleviates when warm, enters the winter heavy.When serious, lassitude, appetite subtracts greatly, does not rise sleepingly, finally dead.
Cattle chorioptic mange is to be caused by cattle foot demodicid mite, multiple being born in below root of the tail, and ball-joint, pastern also can be fallen ill, and spread to the portions such as extremity, breast, perineum, skin eczema sample pathological changes, pruritus.
The general onset of cattle demodicidosis is in head, neck, shoulder, the back of the body and buttocks.Form scabies and be syringe needle greatly so that Semen Juglandis is large, include powder or pus sample liquid.Also have and only occur squama and there is no furuncle.
3, diagnosis: can take the scurf of the intersection of the crust on corresponding pars affecta skin, particularly affected part and healthy portion to check under the microscope, look to have or not worm's ovum and polypide.Psoroptes bovis disease can gather cervical region, angle base, root of the tail, spreads gradually under neck and takes on the portions such as side.Sarcoptes bovis disease can gather head, cervical region and skin of shoulder.Cattle chorioptic mange can gather below root of the tail, ball-joint, pastern skin.Cattle demodicidosis can collection head, neck, shoulder, the back of the body and skin of buttock.
4, prophylactico-therapeutic measures at present: this disease almost spreads all over the country of all rear cattles in the world, is widely distributed.This disease at present conventional medicine to mainly contain organophosphorus insecticide as clean in metrifonate, phoxim, coumafos, demodicid mite etc.; Pyrethroid is as decis; First is narrowed class as Amitraz, ivermectin etc.These drug toxicitys are large, and safety in utilization is poor, easily residual, affect the quality of meat, and much strictly restriction is used.And Western medicine is because its toxic and side effects by force, easily produces the problems such as drug resistance and drug residue, and clinical practice is very limited.And treat at present some Chinese medicines of this disease, and because its prescription is unreasonable or simple pulverizing just, particle diameter is excessive, and drug release is slower, causes its bioavailability lower, and clinical effectiveness is not fine.Therefore research and develop and a kind ofly treat the having no side effect of equus acaricide, drug residue free, have no drug resistance and specific medicine is one of new problem anxious to be resolved.
summary of the invention:
The object of the invention is to overcome the weak point in treatment cattle acaricide at present, providing a kind of draws materials easy, easy to prepare and kills that acarid effect is strong, instant effect, reduce drug residue and toxic and side effects, the externally-applied medicinal composition of cheap treatment cattle acaricide.The present invention also provides its preparation method.
The present invention is achieved by the following technical solutions:
A kind of externally-applied medicinal composition for the treatment of cattle acaricide is to be made by the raw material of following weight portion: Nidus Vespae 30-50 part, Semen Hydnocarpi 20-40 part, Folium Fumicis Dentati 20-40 part, Cortex Pseudolaricis 20-40 part, Fructus Ulmus preparatium 20-40 part, sulfur 10-30 part, Cortex Dictamni 20-40 part, Fructus Kochiae 20-40 part, Zaocys 20-40 part, Pericarpium Granati 20-40 part, Alumen 10-30 part, mature vinegar 140-200 part;
Preferred composition by weight is:
30 parts of Nidus Vespaes, 40 parts of Semen Hydnocarpis, 20 parts of Folium Fumicis Dentatis, 40 parts of Cortex Pseudolariciss, 20 parts of Fructus Ulmus preparatium, 30 parts, sulfur, 20 parts of Cortex Dictamni, 40 parts of the Fructus Kochiae, 20 parts of Zaocyss, 40 parts of Pericarpium Granatis, 10 parts, Alumen, 200 parts, mature vinegar;
Or be:
50 parts of Nidus Vespaes, 20 parts of Semen Hydnocarpis, 40 parts of Folium Fumicis Dentatis, 20 parts of Cortex Pseudolariciss, 40 parts of Fructus Ulmus preparatium, 10 parts, sulfur, 40 parts of Cortex Dictamni, 20 parts of the Fructus Kochiae, 40 parts of Zaocyss, 20 parts of Pericarpium Granatis, 30 parts, Alumen, 140 parts, mature vinegar;
Or be:
40 parts of Nidus Vespaes, 30 parts of Semen Hydnocarpis, 30 parts of Folium Fumicis Dentatis, 30 parts of Cortex Pseudolariciss, 30 parts of Fructus Ulmus preparatium, 20 parts, sulfur, 30 parts of Cortex Dictamni, 30 parts of the Fructus Kochiae, 30 parts of Zaocyss, 30 parts of Pericarpium Granatis, 20 parts, Alumen, 170 parts, mature vinegar.
Another object of the present invention is to provide a kind of externally-applied medicinal composition for the treatment of cattle acaricide, is to be prepared by following method:
1) by above-mentioned weight portion, take Folium Fumicis Dentati, Fructus Ulmus preparatium, Cortex Dictamni, the Fructus Kochiae, Zaocys, Pericarpium Granati, after pulverizing, put into decoction pot, soak 25-35 minute, twice decoction, each 25-35 minute, twice filtrate merges, and concentrate drying is made dry powder;
2) Nidus Vespae, Semen Hydnocarpi, Cortex Pseudolaricis, sulfur, Alumen are pulverized, mixed with mature vinegar, then mix with the dry powder of step 1).
During use, add water furnishing paste or pasty state and be applied to affected part.
The invention discloses a kind of externally-applied medicinal composition for the treatment of cattle acaricide and preparation method thereof, Nidus Vespae in side: bitter, pungent, flat, poisonous, return liver, stomach two warps.Have and dispel the wind, counteracting toxic substances, parasite killing, the merit of pain relieving.Main with controlling the cards such as sore swollen toxin, skin stubborn dermatitis, scrofula.Its attack virus killing worm and analgesic effect obvious, external is usually used in the diseases such as skin pruritus, skin ulcer tinea, acarid.The acaricide of cattle is had to good curative effect.Semen Hydnocarpi: pungent, heat, poisonous.Have and dispel the wind, the merit of parasite killing.External can be treated scabies, is the common drug for the treatment of cattle acarid.Folium Fumicis Dentati: there is heat-clearing and toxic substances removing; The merit of killing parasites for relieving itching.Acute mastitis; Sore swollen toxin; Scabies.Be that oral administration again can external.Cortex Pseudolaricis: pungent, temperature; Poisonous.Return lung, spleen channel.There is parasite killing, antipruritic merit.External is used for scabies pruritus.Fructus Ulmus preparatium: pungent, flat, nontoxic.The merit with eliminating stagnated food and killing intestinal worms.Can treat multiple endoparasite and ectoparasite sick, for infantile malnutrition, ascariasis, enterobiasis.Also there is good antifungic action, various skin fungus is had to inhibitory action in various degree, cattle acaricide is had to good curative effect.Sulfur: acid, temperature.Poisonous.Enter kidney, large intestine channel.There is removing toxic substances killing parasites for relieving itching, mend the merit that fire is supporing yang.Can treat multiple dermatosis.Treatment scabies, the key medicine of the diseases such as carbuncle of yin nature.This product has the effect for the treatment of the poisonous disease with poisonous drugs, and has the merit of parasite killing, and treatment cattle acaricide is normal to be applied with Alumen, Fructus Cnidii compatibility.External solubilized cutin, softening skin, kills acarid, pathogenic bacteria and mycete etc.The Fructus Kochiae: pungent, bitter, cold.Return kidney, urinary bladder channel.There is clearing away heat-damp and promoting diuresis, the merit of dispelling wind for relieving itching.Be usually used in rubella, eczema, the cards such as skin pruritus.Cortex Dictamni: hardship, cold.Return spleen, stomach, urinary bladder channel.There is heat clearing and damp drying, the removing toxic substances of dispeling the wind.Be used for damp and hot sore, yellow fluid is dripping, eczema rubella, the cards such as scabies skin ulcer leprosy.Zaocys: sweet, flat.Return Liver Channel.Have and dispel the wind, dredging collateral, the merit of relieving convulsion.Be used for rheumatoid arthritis stubborn, numbness contracture, leprosy scabies, the cards such as scrofula malignant boil.External coordinates the cards such as Drug therapy urticaria, scabies such as the Fructus Kochiae, Cortex Dictamni to have good curative effect, in addition multiple endoparasite and ectoparasite disease is had to therapeutic effect, and cattle acaricide is had to good therapeutical effect.Pericarpium Granati: sour, puckery, temperature.Return large intestine channel.There is relieving diarrhea with astringents, hemostasis, the merit of anthelmintic.For chronic diarrhea, chronic dysentery, has blood in stool and parasitic disease.Alumen: sour, puckery, cold.Return lung, liver, spleen, large intestine channel.There is removing toxic substances, parasite killing, antipruritic, removing heat-phlegm, the functions such as relieving diarrhea with astringents, oral administration again can external, and external can be treated the cards such as scabies, eczema, eczema, and the compatibility of drugss such as normal and sulfur are treated cattle acaricide.All medicines share and become altogether clearing away heat and expelling wind, the agent that acaricide is antipruritic.
The side's of system feature: we are with heat clearing and damp drying, the removing toxic substances of dispeling the wind, the antipruritic and use of acaricide, holds concurrently with promotion hair growth, prevents courses of infection, treating both the principal and secondary aspects of a disease.
Said medicine is through organic assembling, and dialectical compatibility and mutually assisting a ruler in governing a country has strengthened the party's clearing away heat and expelling wind, the effect that acaricide is antipruritic, thus reach the object of curing cattle acaricide.Adopt that the externally-applied medicinal composition of the treatment cattle acaricide that a kind of preparation method for the treatment of the externally-applied medicinal composition of cattle acaricide of the present invention makes has that cost is low, preparation easily, have no side effect and evident in efficacy.Clinical practice proves, the cure rate 100% of this medicine, effective percentage 100%.
Some medicine in we is toxic, will prevent from suffering from cattle and bite and embrocate the affected part of medicine in therapeutic process.During treatment, first by the cropping of trouble cattle diseased region, scrape off crust and dirt, dry, sterilization after with medicinal swab stick dip in liquid medicine, embrocates affected part, be a course for the treatment of twice, one week every day, take effect a general course for the treatment of, can fully recover two courses for the treatment of.
the specific embodiment:
embodiment 1
30 parts of Nidus Vespaes, 40 parts of Semen Hydnocarpis, 20 parts of Folium Fumicis Dentatis, 40 parts of Cortex Pseudolariciss, 20 parts of Fructus Ulmus preparatium, 30 parts, sulfur, 20 parts of Cortex Dictamni, 40 parts of the Fructus Kochiae, 20 parts of Zaocyss, 40 parts of Pericarpium Granatis, 10 parts, Alumen, 200 parts, mature vinegar.
embodiment 2
50 parts of Nidus Vespaes, 20 parts of Semen Hydnocarpis, 40 parts of Folium Fumicis Dentatis, 20 parts of Cortex Pseudolariciss, 40 parts of Fructus Ulmus preparatium, 10 parts, sulfur, 40 parts of Cortex Dictamni, 20 parts of the Fructus Kochiae, 40 parts of Zaocyss, 20 parts of Pericarpium Granatis, 30 parts, Alumen, 140 parts, mature vinegar.
embodiment 3
Composition by weight: 40 parts of Nidus Vespaes, 30 parts of Semen Hydnocarpis, 30 parts of Folium Fumicis Dentatis, 30 parts of Cortex Pseudolariciss, 30 parts of Fructus Ulmus preparatium, 20 parts, sulfur, 30 parts of Cortex Dictamni, 30 parts of the Fructus Kochiae, 30 parts of Zaocyss, 30 parts of Pericarpium Granatis, 20 parts, Alumen, 170 parts, mature vinegar.
embodiment 4
(1) by embodiment 1,2 or 3 proportionings, weigh.
(2) the various compositions in prescription are pulverized respectively to rear mistake 200 mesh sieves.
(3) Folium Fumicis Dentati, Fructus Ulmus preparatium, Cortex Dictamni, the Fructus Kochiae, Zaocys, Pericarpium Granati in side are put into decoction pot, soak 30 minutes.
(4) the medicine intense fire in decoction pot boiled after, slow fire boiling 30 minutes, leaches medicinal liquid, medicinal residues add water and continue to decoct once.After twice medicinal liquid merged, continue heating and be concentrated into sticky shape, cease fire.
(5), by Nidus Vespae, Semen Hydnocarpi, Cortex Pseudolaricis, sulfur, Alumen mix homogeneously after pulverizing, treat that sticky shape decocts medicinal liquid and is down to 40 0during C, add and wherein become paste, be the externally-applied medicinal composition for the treatment of cattle acaricide.Described umber is weight portion.
embodiment 5
1) by this, execute example 1,2,3 proportionings, take Folium Fumicis Dentati, Fructus Ulmus preparatium, Cortex Dictamni, the Fructus Kochiae, Zaocys, Pericarpium Granati, after pulverizing, put into decoction pot, soak 25-35 minute, twice decoction, each 25-35 minute, twice filtrate merges, and concentrate drying is made dry powder;
2) Nidus Vespae, Semen Hydnocarpi, Cortex Pseudolaricis, sulfur, Alumen are pulverized, crossed 200 mesh sieves, mix with mature vinegar, then mix with the dry powder of step 1).
During use, add water furnishing paste or pasty state and be applied to affected part.
clinical effect trial
1. method: publish cattle farm and the out-patient treatment case of raw cattle acariasis, select altogether 37 of the ill cattle of acarid pathological changes, age 6-13 year.All trouble cattle, all through taking the crust on corresponding pars affecta skin, check and have acarid polypide to exist.Trouble poultry is divided into three groups, is respectively of the present invention group, wipes scabies side's group and matched group.
During treatment, to carry out in ad hoc place, after the personnel of contact trouble poultry palpus thorough disinfection after treatment finishes, replacing medicated clothing, can leave away.First trouble Niu Quanshen is conscientiously scrubbed, in affected part and cropping around thereof, remove dirt and crust, with warm suds, scrub, avoid hemorrhage as far as possible.Scrubbed after cattle body, dried, then through sterilization, then with medicinal swab stick dip in liquid medicine, embrocated affected part, examine, do not had the place of omission.In therapeutic process, the phenomenon that pars affecta skin there will be top layer scurf to come off, must clean out scurf embrocate again, in order to avoid medicinal liquid does not reach skin.Respectively embrocate once morning and afternoon every day, within one week, is a course for the treatment of, and take effect a general course for the treatment of, and can fully recover two courses for the treatment of.Effective percentage 100%, cure rate 100%.
Of the present invention group for pressing the externally-applied medicinal composition of the prepared treatment of embodiment 4 cattle acaricide.
Wipe wiping scabies side, scabies side's group wiping used scabies side and derive from the prosperous horse collection > > that treats of < < unit, it consists of 120 parts of the Radixs Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), 120 parts of Fructus Gleditsiae Abnormalis, 30 parts of Fructus Crotonis, 9 parts of Realgars, 6 parts of Calomelass, be smalls altogether, with deep fat, mix well wiping.The next day once.Effect is killing parasites for relieving itching.Cure mainly scabies.It is traditional prescription for the treatment of acarid.
Matched group need not any Drug therapy.
Treatment time is two courses for the treatment of, and be 7 days each course for the treatment of, and each coating of every day at upper and lower noon once.
Table 1 is suffered from poultry grouping and primary treatment method
Group Suffer from cattle quantity (head) Therapeutic Method
Of the present invention group 16 To after trouble poultry affected part Local treatment, embrocate medicine of the present invention
Wipe scabies side's group 13 To after trouble poultry affected part Local treatment, embrocate and wipe scabies side's medicine
Matched group 8 Suffering from poultry affected part part is left intact
---------------------be efficacy determination 2.:
Criterion of therapeutical effect: whether the symptom of efficacy determination Main Basis cattle acaricide disappears.Medication 7 days is a course for the treatment of, observe the curative effect after two courses for the treatment of.Concrete curative effect is divided into three grades:
Cure: cattle acaricide symptom all disappears;
Take a turn for the better: cattle acaricide symptom partly disappears;
Invalid: cattle acaricide symptom is without significant change.
3. result: suffer from cattle by different dosage regimens for 37, after the treatment of two courses for the treatment of, each therapeutic effect of organizing is in Table 2.
Niu Xiaoguo is suffered from the various therapeutic scheme treatments of table 2
Group Treatment quantity Cure Take a turn for the better Invalid Cure rate (%) Total effective rate (%)
Of the present invention group 16 16 0 0 100.00 100.00
Wipe scabies side's group 13 8 3 2 61.54 84.62
Matched group 8 0 2 6 0 25.00
From table 2, two groups of medicines have different therapeutic effect to cattle acaricide, and wherein best with of the present invention group of therapeutic effect, cure rate reaches 100.00%, total effective rate 100.00%, and the utmost point is significantly higher than other two groups (P < 0. 01); Wipe scabies side's group and take second place, and matched group does not have therapeutical effect.Statistical result shows, each group difference is (P< 0. 01) extremely significantly.
Facts have proved, medicine of the present invention is through organic assembling, and dialectical compatibility and mutually assisting a ruler in governing a country has strengthened the party's clearing away heat and expelling wind, and the effect that acaricide is antipruritic, has good curative effect for cattle acaricide.Thereby reach the object of healing.Adopt that the externally-applied medicinal composition of the treatment cattle acaricide that a kind of preparation method for the treatment of the externally-applied medicinal composition of cattle acaricide of the present invention makes has that cost is low, preparation easily, have no side effect and evident in efficacy.For this disease of clinical treatment provides a kind of practicable new drug.Clinical practice proves, the cure rate 100.00% of this medicine, effective percentage 100%.
Above-mentioned detailed description of a kind of externally-applied medicinal composition for the treatment of cattle acaricide of this treatment and preparation method thereof being carried out with reference to embodiment; illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not depart under general plotting of the present invention, within should belonging to protection scope of the present invention.

Claims (4)

1. treating an externally-applied medicinal composition for cattle acaricide, is to be made by the raw material of following weight portion: Nidus Vespae 30-50 part, Semen Hydnocarpi 20-40 part, Folium Fumicis Dentati 20-40 part, Cortex Pseudolaricis 20-40 part, Fructus Ulmus preparatium 20-40 part, sulfur 10-30 part, Cortex Dictamni 20-40 part, Fructus Kochiae 20-40 part, Zaocys 20-40 part, Pericarpium Granati 20-40 part, Alumen 10-30 part, mature vinegar 140-200 part;
Preparation method is as follows:
1) take Folium Fumicis Dentati, Fructus Ulmus preparatium, Cortex Dictamni, the Fructus Kochiae, Zaocys, Pericarpium Granati, after pulverizing, put into decoction pot, soak 25-35 minute, twice decoction, each 25-35 minute, twice filtrate merges, and concentrate drying is made dry powder;
2) Nidus Vespae, Semen Hydnocarpi, Cortex Pseudolaricis, sulfur, Alumen are pulverized, mixed with mature vinegar, then mix with the dry powder of step 1).
2. a kind of externally-applied medicinal composition for the treatment of cattle acaricide according to claim 1, is characterized in that: 30 parts of Nidus Vespaes, 40 parts of Semen Hydnocarpis, 20 parts of Folium Fumicis Dentatis, 40 parts of Cortex Pseudolariciss, 20 parts of Fructus Ulmus preparatium, 30 parts, sulfur, 20 parts of Cortex Dictamni, 40 parts of the Fructus Kochiae, 20 parts of Zaocyss, 40 parts of Pericarpium Granatis, 10 parts, Alumen, 200 parts, mature vinegar.
3. a kind of externally-applied medicinal composition for the treatment of cattle acaricide according to claim 1, is characterized in that: 50 parts of Nidus Vespaes, 20 parts of Semen Hydnocarpis, 40 parts of Folium Fumicis Dentatis, 20 parts of Cortex Pseudolariciss, 40 parts of Fructus Ulmus preparatium, 10 parts, sulfur, 40 parts of Cortex Dictamni, 20 parts of the Fructus Kochiae, 40 parts of Zaocyss, 20 parts of Pericarpium Granatis, 30 parts, Alumen, 140 parts, mature vinegar.
4. a kind of externally-applied medicinal composition for the treatment of cattle acaricide according to claim 1, is characterized in that: 40 parts of Nidus Vespaes, 30 parts of Semen Hydnocarpis, 30 parts of Folium Fumicis Dentatis, 30 parts of Cortex Pseudolariciss, 30 parts of Fructus Ulmus preparatium, 20 parts, sulfur, 30 parts of Cortex Dictamni, 30 parts of the Fructus Kochiae, 30 parts of Zaocyss, 30 parts of Pericarpium Granatis, 20 parts, Alumen, 170 parts, mature vinegar.
CN201310040251.8A 2013-02-01 2013-02-01 Externally used medicinal composition for treating cattle mange and preparation method thereof Expired - Fee Related CN103070951B (en)

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