CN103060231A - Composite microecological bactericide used for eliminating stink in culturing farm - Google Patents
Composite microecological bactericide used for eliminating stink in culturing farm Download PDFInfo
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- CN103060231A CN103060231A CN2012105751351A CN201210575135A CN103060231A CN 103060231 A CN103060231 A CN 103060231A CN 2012105751351 A CN2012105751351 A CN 2012105751351A CN 201210575135 A CN201210575135 A CN 201210575135A CN 103060231 A CN103060231 A CN 103060231A
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Abstract
The invention provides a composite microecological bactericide used for eliminating stink in a culturing farm. The composite microecological bactericide is prepared by inoculating a lactic acid bacterium, a nitrobacterium and Bacillus spp according to a ratio of 2: 1: 1 into a bacterium culture medium for shaking composite culture, then preparing a primary deodorizing bacterial stock solution and finally carrying out a series of operations. The invention has the following advantages: the content of ammonia and hydrogen sulfide in the air is effectively reduced, so an obvious effect on elimination of stink in the culturing farm is exerted; and the characteristics of a simple process and low cost are obtained.
Description
[technical field]
The present invention relates to a kind of microecological microbial agent of eliminating stink, relate in particular to a kind of compound microecological microbial inoculum of eliminating stink for plant.
[background technology]
Repugnant substance is of a great variety, different osmophore in the dissimilar material molecular structures, thereby different smells and threshold values are arranged.To the larger thio-alcohol of human health damage, ammonia, hydrogen sulfide, dimethyl sulphide, Trimethylamine 99, formaldehyde, vinylbenzene, chromic acid and phenols etc., and the repugnant substance that existing plant produces mainly contains ammonia, hydrogen sulfide in repugnant substance.Respiratory system, the recycle system, Digestive tract, endocrine system and the neural system of odor pollution primary hazard human body have had a strong impact on people's the mental status, have reduced quality of life.
Raising along with China's living standards of the people, the discharging of stench constantly increases the weight of for the pollution of environment, the foul smell that produces constantly aggravates the pollution of environment, health to people causes great threat, odor pollution more and more causes people's attention, therefore, eliminate material (the being reodorant) exploitation that stink uses and use a focus that has become research.
Reodorant mainly is divided into physical reodorant, chemotype reodorant, microbiocidal reodorant etc.Physical reodorant is to carry out deodorizing by physical method, does not change the result of odor components, only changes the partial concn of foul smell, or perhaps relative concentration; Thereby chemotype reodorant is to utilize oxidation, reduction decomposition, neutralization reaction, addition reaction, condensation reaction, ion exchange reaction etc. that the repugnant substance that produces is become the odorless material to destroy foul odors; Microbiocidal reodorant is to utilize microorganism that the repugnant substance that is dissolved in the water is absorbed in microorganism in health, and the Metabolic activity by microorganism makes its degraded.
Wherein, microbiocidal reodorant more and more is subject to people's attention because it has the advantage of environmental protection.But existing microbiocidal reodorant production technique is comparatively complicated, and the compound microecological microbial inoculum is difficult for obtaining, the poor effect of degraded ammonia and hydrogen sulfide, and these have all limited the further application of microbiocidal reodorant.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound microecological microbial inoculum for plant's elimination stink, and not only efficiency ground reduces the content of Ammonia in Air and hydrogen sulfide, and also has the advantage that technique is comparatively simple, cost is low.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound microecological microbial inoculum for plant's elimination stink, and the concrete steps of its preparation method are as follows:
(1) preparation composite bacteria stoste: milk-acid bacteria, nitrifier, genus bacillus are seeded in the yeast culture base by the bacterium amount ratio of 2:1:1, and in 28 ℃ of lower shaking table shaking culture 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under normal temperature after shaking culture finishes, in the process of static cultivation, get at set intervals an amount of nutrient solution and measure its compound total viable count, then static cultivation finishes when the compound total viable count of measuring reaches 100,000,000/milliliter, gets required composite bacteria stoste;
(2) the elementary deodorizing bacterium stoste of preparation: get the sewage in plant methane-generating pit exit, with the dilution of the pure water of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to get mixed solution; Get this mixed solution of 10ml to clean test tube, the composite bacteria stoste that adds 1ml step (1) gained, shake up 3 minutes and be placed on 28 ℃ of lower constant temperature culture, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless, and pH value when being 4 ~ 5, finish constant temperature culture and also liquid is put normal temperature then make elementary deodorizing bacterium stoste more than 72 hours, for subsequent use;
(3) preparation compound microecological microbial inoculum: get the sewage in plant methane-generating pit exit, for subsequent use; Get described sewage and mix 1 grade of substratum mixed solution of formation with the yeast culture base by the weight ratio of 1:99, in this 1 grade of substratum mixed solution, add the prepared elementary deodorizing bacterium stoste of step (2), and the weight ratio of this that adds elementary deodorizing bacterium stoste and this 1 grade of substratum mixed solution is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 1 grade of deodorizing bacterium stoste; Get afterwards described sewage and mix 2 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 2:98, the 1 grade of deodorizing bacterium stoste that in these 2 grades of substratum mixed solutions, adds gained, and the weight ratio of this that adds 1 grade of deodorizing bacterium stoste and these 2 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 2 grades of deodorizing bacterium stostes; Then get described sewage and mix 3 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 4:96,2 grades of deodorizing bacterium stostes that in these 3 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 2 grades of deodorizing bacterium stoste and these 3 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 3 grades of deodorizing bacterium stostes; Then get described sewage and mix 4 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 6:94,3 grades of deodorizing bacterium stostes that in these 4 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 3 grades of deodorizing bacterium stoste and these 4 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 4 grades of deodorizing bacterium stostes; Get described sewage and mix 5 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 10:90,4 grades of deodorizing bacterium stostes that in these 5 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 4 grades of deodorizing bacterium stoste and these 5 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observe the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 5 grades of deodorizing bacterium stostes; Getting at last described 5 grades of substratum mixed solutions mixes by the weight ratio of 95:5 with 5 grades of deodorizing bacterium stostes, mix rearmounted 28 ℃ of lower airtight constant temperature culture, observe the process of constant temperature culture and also measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and get described compound microecological microbial inoculum during the flavor acid alcohol.
Further, the component of described yeast culture base is: ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract paste 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; Per-cent in this yeast culture base component all is weight percentage.
Further, the method for described mensuration bacterium number is to adopt the ascites method.
The beneficial effect that the present invention is used for the compound microecological microbial inoculum of plant's elimination stink is: not only efficiency ground reduces the content of Ammonia in Air and hydrogen sulfide, to play the effect of eliminating significantly stink in the plant (such as the pig farm), and this compound microecological microbial inoculum also has the advantage that technique is comparatively simple, cost is low, thereby so that compound microecological microbial inoculum of the present invention can use comparatively widely.
[embodiment]
The present invention is used for the compound microecological microbial inoculum that stink is eliminated by plant, and the concrete steps of its preparation method are as follows:
(1) preparation composite bacteria stoste: milk-acid bacteria, nitrifier, genus bacillus are seeded in the yeast culture base by the bacterium amount ratio of 2:1:1, and in 28 ℃ of lower shaking table shaking culture 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under normal temperature after shaking culture finishes, in the process of static cultivation, get at set intervals an amount of nutrient solution and measure its compound total viable count, then static cultivation finishes when the compound total viable count of measuring reaches 100,000,000/milliliter, gets required composite bacteria stoste;
(2) the elementary deodorizing bacterium stoste of preparation: get the sewage in plant methane-generating pit exit, with the dilution of the pure water of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to get mixed solution; Get this mixed solution of 10ml to clean test tube, the composite bacteria stoste that adds 1ml step (1) gained, shake up 3 minutes and be placed on 28 ℃ of lower constant temperature culture, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless, and pH value when being 4 ~ 5, finish constant temperature culture and also liquid is put normal temperature then make elementary deodorizing bacterium stoste more than 72 hours, for subsequent use;
(3) preparation compound microecological microbial inoculum: get the sewage in plant methane-generating pit exit, for subsequent use; Get described sewage and mix 1 grade of substratum mixed solution of formation with the yeast culture base by the weight ratio of 1:99, in this 1 grade of substratum mixed solution, add the prepared elementary deodorizing bacterium stoste of step (2), and the weight ratio of this that adds elementary deodorizing bacterium stoste and this 1 grade of substratum mixed solution is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 1 grade of deodorizing bacterium stoste; Get afterwards described sewage and mix 2 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 2:98, the 1 grade of deodorizing bacterium stoste that in these 2 grades of substratum mixed solutions, adds gained, and the weight ratio of this that adds 1 grade of deodorizing bacterium stoste and these 2 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 2 grades of deodorizing bacterium stostes; Then get described sewage and mix 3 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 4:96,2 grades of deodorizing bacterium stostes that in these 3 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 2 grades of deodorizing bacterium stoste and these 3 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 3 grades of deodorizing bacterium stostes; Then get described sewage and mix 4 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 6:94,3 grades of deodorizing bacterium stostes that in these 4 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 3 grades of deodorizing bacterium stoste and these 4 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 4 grades of deodorizing bacterium stostes; Get described sewage and mix 5 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 10:90,4 grades of deodorizing bacterium stostes that in these 5 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 4 grades of deodorizing bacterium stoste and these 5 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observe the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 5 grades of deodorizing bacterium stostes; Getting at last described 5 grades of substratum mixed solutions mixes by the weight ratio of 95:5 with 5 grades of deodorizing bacterium stostes, mix rearmounted 28 ℃ of lower airtight constant temperature culture, observe the process of constant temperature culture and also measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and get described compound microecological microbial inoculum during the flavor acid alcohol.
Wherein, the component of the yeast culture base in the above steps is: ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract paste 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; Per-cent in this yeast culture base component all is weight percentage.
Compound microecological microbial inoculum of the present invention be used for to be eliminated the stink of plant, and the applicant is applied to the pig farm take this compound microecological microbial inoculum and sets forth explanation as example, particularly:
Adopt 10 volumes water doubly to dilute to obtain 10 times of water diluents the above-mentioned compound microecological microbial inoculum for preparing, with the place that these 10 times of water diluents are sprayed on the ground equably and fecaluria is concentrated, ammonia in the mensurated gas composition after 5 minutes, and the concentration of hydrogen sulfide, and ammonia in gas before 10 times of water diluents are sprayed, and the concentration of hydrogen sulfide measure; Wherein gas sampling is with CD-1 type portable type air sampler, and in the column home on pig farm 5 point samplings, average repeated experiments 5 times, experimental result such as following table 1.
Table 1: the degradation effect of ammonia and hydrogen sulfide
And as shown in Table 1, the compound micro-ecological preparation of deodorizing special use of the present invention can make ammonia, hydrogen sulfide degrade respectively 72.2%, 81.8%, and Ammonia in Air and hydrogen sulfide on average are down to respectively 0.168mg/m in the pig farm
3And 0.039mg/m
3
To sum up, the compound micro-ecological preparation of the deodorizing special use that the present invention makes can reduce the content of Ammonia in Air and hydrogen sulfide effectively, play the effect of eliminating significantly stink in the plant (such as the pig farm), and this compound microecological microbial inoculum also has the advantage that production technique is comparatively simple, cost is low, thereby so that compound microecological microbial inoculum of the present invention can use comparatively widely.
Claims (3)
1. one kind is used for the compound microecological microbial inoculum that stink is eliminated by plant, and it is characterized in that: the concrete steps of its preparation method are as follows:
(1) preparation composite bacteria stoste: milk-acid bacteria, nitrifier, genus bacillus are seeded in the yeast culture base by the bacterium amount ratio of 2:1:1, and in 28 ℃ of lower shaking table shaking culture 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under normal temperature after shaking culture finishes, in the process of static cultivation, get at set intervals an amount of nutrient solution and measure its compound total viable count, then static cultivation finishes when the compound total viable count of measuring reaches 100,000,000/milliliter, gets required composite bacteria stoste;
(2) the elementary deodorizing bacterium stoste of preparation: get the sewage in plant methane-generating pit exit, with the dilution of the pure water of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to get mixed solution; Get this mixed solution of 10ml to clean test tube, the composite bacteria stoste that adds 1ml step (1) gained, shake up 3 minutes and be placed on 28 ℃ of lower constant temperature culture, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless, and pH value when being 4 ~ 5, finish constant temperature culture and also liquid is put normal temperature then make elementary deodorizing bacterium stoste more than 72 hours, for subsequent use;
(3) preparation compound microecological microbial inoculum: get the sewage in plant methane-generating pit exit, for subsequent use; Get described sewage and mix 1 grade of substratum mixed solution of formation with the yeast culture base by the weight ratio of 1:99, in this 1 grade of substratum mixed solution, add the prepared elementary deodorizing bacterium stoste of step (2), and the weight ratio of this that adds elementary deodorizing bacterium stoste and this 1 grade of substratum mixed solution is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 1 grade of deodorizing bacterium stoste; Get afterwards described sewage and mix 2 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 2:98, the 1 grade of deodorizing bacterium stoste that in these 2 grades of substratum mixed solutions, adds gained, and the weight ratio of this that adds 1 grade of deodorizing bacterium stoste and these 2 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 2 grades of deodorizing bacterium stostes; Then get described sewage and mix 3 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 4:96,2 grades of deodorizing bacterium stostes that in these 3 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 2 grades of deodorizing bacterium stoste and these 3 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 3 grades of deodorizing bacterium stostes; Then get described sewage and mix 4 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 6:94,3 grades of deodorizing bacterium stostes that in these 4 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 3 grades of deodorizing bacterium stoste and these 4 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observed the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 4 grades of deodorizing bacterium stostes; Get described sewage and mix 5 grades of substratum mixed solutions of formation with the yeast culture base by the weight ratio of 10:90,4 grades of deodorizing bacterium stostes that in these 5 grades of substratum mixed solutions, add gained, and the weight ratio of this that adds 4 grades of deodorizing bacterium stoste and these 5 grades of substratum mixed solutions is 5:95, stir and be placed on 28 ℃ of lower constant temperature culture in 5 minutes, after 48 hours, observe the process of constant temperature culture and measured its contained bacterium number every 24 hours, when the bacterium number of measuring reaches 100,000,000/milliliter, get 5 grades of deodorizing bacterium stostes; Getting at last described 5 grades of substratum mixed solutions mixes by the weight ratio of 95:5 with 5 grades of deodorizing bacterium stostes, mix rearmounted 28 ℃ of lower airtight constant temperature culture, observe the process of constant temperature culture and also measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and get described compound microecological microbial inoculum during the flavor acid alcohol.
2. the compound microecological microbial inoculum of eliminating stink for plant according to claim 1, it is characterized in that: the component of described yeast culture base is: ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract paste 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; Per-cent in this yeast culture base component all is weight percentage.。
3. the compound microecological microbial inoculum of eliminating stink for plant according to claim 1, it is characterized in that: the method for described mensuration bacterium number is to adopt the ascites method.
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Application publication date: 20130424 |