CN103045686B - Comprehensive utilization method of lignocellulose biomass - Google Patents
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Abstract
A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; (b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2011195; (c) processing the enzymatic hydrolysis residues produced in step (b) by using an alkaline solution so as to extract lignin from the enzymatic hydrolysis residues. The method realizes the maximum resource utilization of lignocellulose biomass.
Description
Technical field
The present invention relates to a kind of method that fully utilizes lignocellulose biomass, specifically a kind of method that fully utilizes Mierocrystalline cellulose in lignocellulose biomass, hemicellulose and xylogen.
Background technology
Day by day serious along with the increasingly exhausted and environmental pollution of fossil fuel resource, utilizes the substitute that the renewable energy resources are petroleum chemicals to become further important.And alcohol fuel is the principal mode of the material of biomass liquid energy, it is also the most probable substitute of fossil oil.At present, world's alcohol production is mainly usingd starch based (corn, cassava etc.) and carbohydrate (sugarcane, the beet etc.) raw material as fermentation.Adopt microbial method method fermentative production of ethanol technology maturation, but high raw materials cost is restricted the industrial application of grain fermentative production of ethanol, exist simultaneously with people strive grain and grain strive etc. drawback, and cause provision price Continued, so it is imperative to find new raw material.Present scientist is more cheap sight trend of purchasing cost, the lignocellulose biomass widely of originating.
Lignocellulose biomass exists with the form of plant materials, main component is Mierocrystalline cellulose, hemicellulose and xylogen, wherein, Mierocrystalline cellulose accounts for 40% left and right, hemicellulose accounts for 25% left and right, xylogen accounts for 20% left and right, and the lignocellulose biomass total amount being generated by photosynthesis every year on the earth surpasses 2,000 hundred million tons, so Wooden Biomass is renewable resources abundant, the most cheap on the earth.
If can take lignocellulose biomass as raw material production ethanol, the mankind's energy problem will greatly be solved, but the greatest problem that the lignocellulose biomass of take runs in the process of raw material production ethanol is the low conversion rate of cellulase hydrolysis, the high cost of enzymolysis (accounting for the 40-50% of total cost of production), production cost is too high, cannot really realize industrialization.The reason of the low conversion rate of cellulase hydrolysis is: hemicellulose is combined between Mierocrystalline cellulose and xylogen as molecule tamanori on the one hand, and the reticulated structure that xylogen has, as support frame, surround and add set Mierocrystalline cellulose and hemicellulose, xylogen and hemicellulose spatially can hinder contacting of cellulosic molecule and enzyme, enzyme accessibility is poor, has increased the difficulty of enzymolysis.Therefore be necessary lignocellulose biomass to carry out effective pre-treatment, destroy the spatial obstacle of xylogen and hemicellulose, also to avoid pre-treatment to produce simultaneously and be unfavorable for the enzyme inhibitor (as furfural, acetic acid etc.) of enzymolysis, thereby be conducive to cellulosic enzymolysis; On the other hand, cellulase is low to crystalline cellulose enzymatic reaction vigour, therefore, in order to improve the transformation efficiency of cellulase hydrolysis, need to improve the vigor of cellulase.
Summary of the invention
For this reason, technical problem to be solved by this invention is to overcome prior art when comprehensive utilization hemicellulose, xylogen and Mierocrystalline cellulose, the problem of cellulase hydrolysis low conversion rate, thus the lignocellulose biomass method of comprehensive utilization that a kind of cellulase hydrolysis transformation efficiency is high is proposed.
For achieving the above object, the invention provides a kind of method of comprehensive utilization of lignocellulose biomass, comprise the following steps:
(a) lignocellulose biomass is carried out to acid hydrolysis, after separation, obtain pentose solution and acid hydrolysis residue;
(b) use cellulase to carry out enzymolysis to acid hydrolysis residue described in step (a), obtain glucose solution and enzymolysis residue; Described cellulase is for being cultivated the cellulase obtaining by a penicillium, this Penicillium notatum Classification And Nomenclature is PenicilliumdecumbensPD-G3-08, be preserved in Wuhan University's Chinese Typical Representative culture collection center (being called for short CCTCC), depositary institution address: Wuhan University's preservation center.The numbering of registering on the books is CCTCC M 2011195, and preservation date is on June 13rd, 2011.
(c) the described enzymolysis residue producing with alkaline solution treatment step (b), thus the xylogen in described enzymolysis residue extracted.
In the present invention, there is no particular limitation for the kind of acid solution described in acid hydrolysis process, can be that lignocellulose biomass carries out the acid-hydrolyzed conventional acid of using, for example, can be one or more in sulfuric acid, hydrochloric acid, nitric acid and phosphoric acid.In acid hydrolysis process, obtained parameter is also not particularly limited, and can be that lignocellulose biomass carries out acid-hydrolyzed conventional parameter.
Described acid-hydrolyzed temperature is 100-150 ℃, time is 0.5-3 hour, while carrying out described acid hydrolysis, the concentration of acid solution is that (as the acid of selecting is strong acid to 0.5-30 % by weight, the concentration of acid solution is lower, is about 0.5-5 % by weight, as the acid of selecting is weak acid, the concentration of acid solution is higher, is about 5-30 % by weight).The concentration of preferably phosphoric acid is 1-20 % by weight.
Described acid solution is phosphoric acid solution, and concentration is 1-20 % by weight.
Described lignocellulose biomass can be one or more of maize straw, wheat straw, rice straw, bagasse, cotton bavin, cotton seed hull, corn cob, straw, kaoliang stalk, broad-leaved wood and wood chip.
According to raw material condition, carry out pre-treatment, lignocellulose biomass raw material is cut or pulverized, then this stalk section is carried out to scrubbing dust collection.
The condition of described cellulase hydrolysis is: substrate consumption is 80-150g/L, and the addition of cellulase is 10-15FPU/g Mierocrystalline cellulose, and temperature is that 45-55 ℃, pH are that 4-6, mixing speed are 50-200rpm, and enzymolysis transformation time is 2-7 days.
Described alkaline solution is processed and is carried out at 40-100 ℃.
In described alkaline solution processing, liquid-solid volume ratio is 5: 1-20: 1.
In described alkaline solution processing, the concentration of alkaline solution is 5-8 % by weight.
The time that described alkaline solution is processed is 1-6 hour.
Various alkali may be used to the present invention, includes but not limited to aqueous sodium hydroxide solution, potassium hydroxide aqueous solution, ammoniacal liquor etc.But, according to some preferred embodiment, the aqueous solution that alkaline solution is sodium hydroxide.
Technique scheme of the present invention compared with prior art has the following advantages:
1, the present invention has adopted first acid hydrolysis, enzymolysis again, the operational path of last alkaline hydrolysis, because described cellulase is for being cultivated the cellulase obtaining by a penicillium, this Penicillium notatum Classification And Nomenclature is Penicillium decumbens PD-G3-08, has been preserved in Wuhan University's Chinese Typical Representative culture collection center, and its deposit number is CCTCC M 2011195, the cellulase that adopts this Penicillium notatum to produce has higher vigor, has further improved the extraction yield of cellulase hydrolysis; In addition, what the present invention adopted is first acidolysis, enzymolysis again, the operational path of last alkaline hydrolysis, due to acidolysis complete after residue be subacidity, so do not need the first acidolysis of picture, the operational path of alkaline hydrolysis, last enzymolysis again, before enzymolysis, also need substrate to neutralize, make its pH value reach 4-6, therefore, simplify production technique, reduced the pollution to environment; Secondly, owing to having adopted first acid hydrolysis, the technique of the last alkaline hydrolysis of enzymolysis again, so the main component in enzymolysis residue is xylogen, therefore, alkaline solution extracts alkali lignin ratio and is easier to, and has reduced the consumption of alkaline solution, has also reduced the pollution to environment;
As can be seen here, by aforesaid method of the present invention, solved the problem of complex utilization of lignocellulose biomass in prior art, made the utilization of resources reach maximization.
What 2, the present invention was used cultivates by Penicillium notatum the cellulase obtaining, at substrate consumption, be 80-150g/L, the addition of cellulase is 10-15FPU/g Mierocrystalline cellulose, temperature is that 45-55 ℃, pH are that 4-6, mixing speed are 50-200rpm, under the enzymolysis conversion condition of 2-7 days, enzymolysis transformation efficiency is the highest.
3, the temperature of described acid hydrolytic reaction is 100-150 ℃, and the time is 0.5-3 hour, under this temperature and time, can must be more thorough by hydrolysis of hemicellulose, and can stop again under acidic conditions high temperature and reaction times long to xylogen and cellulosic destruction.
4, acid hydrolysis of the present invention acid used is phosphoric acid solution, and the concentration of phosphoric acid solution is while being 1-20 % by weight, has avoided to greatest extent xylogen and cellulosic destruction, and due to phosphoric acid corrosion a little less than, therefore, maintenance of the equipment is simple, duration of service is long.
5, liquid-solid ratio, alkali consumption, temperature and time that the condition that alkaline solution of the present invention is processed adopts, the activity that finally obtains alkali lignin is very high.
Accompanying drawing explanation
For content of the present invention is more likely to be clearly understood, below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein:
Fig. 1 is the schematic diagram of technical process of the present invention.
Embodiment
Below will by specific embodiment, the invention will be further described.
The self-control cellulase that following examples are used is cultivated and is obtained by Penicillium notatum, and concrete cultural method is:
(A) bacterial classification multiplication culture
By naming number, being PenicilliumdecumbensPD-G3-08 Penicillium notatum seed liquor is linked in the fermentor tank that contains seed culture medium through 121 ℃ of sterilizing 30min and activates with the inoculum size of 5% (v/v), keep tank pressure 0.02-0.05MPa, air flow 0.5vvm, mixing speed 100-150rpm, 30 ℃ of cultivation 30-60 hour, the seed liquor after being activated.
Component in described seed culture medium and consumption are: get embodiment 1 acid hydrolysis residue 10-30g/L, wheat bran 20-50g/L, peptone 1-4g/L, ammonium sulfate 2-4g/L, all the other are water.
Component and consumption in described seed culture medium are preferably: acid hydrolysis residue 20g/L, wheat bran 40g/L, peptone 3g/L, ammonium sulfate 3g/L, all the other are water.
(B) prepare cellulase
Inoculum size by step (A) acquisition seed liquor with 10% (v/v) accesses in the 5L fermentor tank that 3L fermention medium is housed of sterilizing, in fermenting process, add defoamer and control foaming, keep tank pressure 0.02-0.05MPa, air flow 0.5-0.6vvm, mixing speed 100-150rpm, 30 ℃ of cultivation 80-136 hour, obtain fermented liquid.
In described fermention medium, each amounts of components is respectively: acid hydrolysis residue 30-50g/L, wheat bran 20-50g/L, Microcrystalline Cellulose or carboxymethyl cellulose 4-8g/L, ammonium sulfate 2-5g/L, potassium primary phosphate 2-4g/L, magnesium sulfate 0.4-0.6g/L, all the other are water, and the initial pH of substratum is 5.0-6.0.
In described fermention medium, each amounts of components is preferably: acid hydrolysis residue 45g/L, wheat bran 35g/L, Microcrystalline Cellulose 5g/L, ammonium sulfate 4g/L, potassium primary phosphate 3g/L, magnesium sulfate 0.6g/L, all the other are water, and the initial pH of substratum is 5.0-6.0.
The fermented liquid 8000rpm centrifuging and taking obtaining obtains supernatant liquor, must contain the crude enzyme liquid of cellulase, and this crude enzyme liquid can be directly used in cellulosic enzymolysis.
(2) test as follows the various performances of xylogen in following examples
The mensuration of content of lignin: comprise sour insoluble xylogen and sour solvable xylogen.Wherein the mensuration of sour insoluble xylogen adopts Klason method, according to GB GB/T2677.8-94, carries out; The solvable xylogen of acid carries out according to GB GB 10337-89.
The mensuration of moisture: carry out according to GB/T 2667.3-93.
Following examples can be referring to Fig. 1.
In following examples, pressure corresponding to acid hydrolysis temperature is the pressure of saturated vapor, therefore no longer for each embodiment provides pressure data.
In following examples, outside specified otherwise, percentage composition used all represents weight percentage, i.e. " % " expression " % by weight ".
Embodiment 1
(1) acid hydrolysis
By 10.6kg corn cob, (mass component forms: moisture 6.12%, Mierocrystalline cellulose 35.19%, hemicellulose 32.1%, xylogen 23.7%, other is 2.95% years old, lower same) smash, wash dedusting with water, then with 80kg phosphoric acid solution, be hydrolyzed, the mass concentration of phosphoric acid solution is 15%, acid-hydrolyzed temperature is 140 ℃, time is 0.5 hour, acid hydrolysis residue and pentose solution that rear separation obtains have been hydrolyzed, with 10kg water, clean described acid hydrolysis residue, scavenging solution and described pentose solution merge, (water content is 65% left and right finally to obtain 17.9kg acid hydrolysis residue, the over dry content of hemicellulose is 13.03%, the over dry content of xylogen is 32.67%, cellulosic over dry content is 49.27%) and 82.07kg pentose solution, the concentration of pentose solution is 3.16%, the extraction yield of hemicellulose is 76%.
The calculation formula of hemicellulose extraction yield is as follows:
The extraction yield %=of hemicellulose (concentration of the quality * pentose of pentose solution)/(content of hemicellulose in corn cob quality * corn cob) * 100%.
(2) cellulase hydrolysis
Get the described acid hydrolysis residue of step (1) as fibrous substrates, carry out cellulase hydrolysis, the condition of described enzymolysis is: cellulase is above-mentioned penicillin (Penicillium decumbens PD-G3-08, be preserved in Wuhan University's Chinese Typical Representative culture collection center, its deposit number is CCTCCM2011195, cultivate the cellulase obtaining down together), according to the cellulosic addition of 15FPU/g, add cellulase, cellulosic substrate consumption is 125g/L, in temperature, it is 48 ℃, pH is 5.0, under the condition of mixing speed 50rpm, enzymolysis transforms 2 days, whole enzymolysis process is without pressurize, obtain glucose solution, quality is 50.15kg, concentration is 4.26%, cellulosic extraction yield is 57%.
The calculation formula of Mierocrystalline cellulose extraction yield is as follows:
Cellulosic extraction yield %=(concentration of the quality * glucose solution of glucose solution)/(cellulosic content in corn cob quality * corn cob) * 100%.
The technique that glucose solution is produced ethanol is existing technique, does not repeat them here, and other embodiment is identical.
(3) alkaline solution extracts alkali lignin
The whole enzymolysis residues that obtain in the present embodiment step (2) and sodium hydroxide solution are mixed, wherein liquid-solid volume ratio is 5: 1, the concentration of sodium hydroxide is 6%, be warming up to 70 ℃, boiling alkaline hydrolysis through 1 hour, separation obtains alkaline hydrolysis residue and alkali lignin solution, with 10kg water, cleans described alkaline hydrolysis residue, and scavenging solution and described alkali lignin solution merge; Finally obtain 28.11kg alkali lignin solution and 6.68kg alkaline hydrolysis residue (water ratio is 65% left and right); Alkali lignin content in alkali lignin solution is 6.38%, and the extraction yield of alkali lignin is 71%.
The formula of alkali lignin extraction yield is as follows:
The extraction yield %=of alkali lignin (content of lignin in quality * alkali lignin solution of alkali lignin solution)/(content of xylogen in corn cob quality * corn cob) * 100%.
Embodiment 2
(1) acid hydrolysis
10.6kg corn cob is smashed, wash dedusting with water, then with 80kg phosphoric acid solution, be hydrolyzed, the mass concentration of phosphoric acid solution is 20%, acid-hydrolyzed temperature is 100 ℃, time is 2 hours, acid hydrolysis residue and pentose solution that rear separation obtains have been hydrolyzed, with 10kg water, clean described acid hydrolysis residue, scavenging solution and described pentose solution merge, (water content is 65% left and right finally to obtain 16.42kg acid hydrolysis residue, the over dry content of hemicellulose is 11.86%, the over dry content of xylogen is 32.72%, cellulosic over dry content is 49.95%) and 83.56kg pentose solution, the concentration of pentose solution is 3.26%, the extraction yield of hemicellulose is 80%.
(2) cellulase hydrolysis
Get the described acid hydrolysis residue of step (1) as fibrous substrates, carry out cellulase hydrolysis, the condition of described enzymolysis is: cellulase is that above-mentioned penicillin (Penicillium decumbens PD-G3-08) is cultivated the cellulase obtaining, according to the cellulosic addition of 10FPU/g, add cellulase, cellulosic substrate consumption is 150g/L, in temperature, it is 55 ℃, pH is 4, under the condition of mixing speed 200rpm, enzymolysis transforms 7 days, whole enzymolysis process obtains glucose solution without pressurize, quality is 44.06kg, concentration is 5.36%, cellulosic extraction yield is 63%.
(3) alkaline solution extracts alkali lignin
The whole enzymolysis residues that obtain in the present embodiment step (2) and sodium hydroxide solution are mixed, wherein liquid-solid volume ratio is 20: 1, the concentration of sodium hydroxide is 5%, then be warming up to 100 ℃, boiling alkaline hydrolysis through 2 hours, separation obtains alkaline hydrolysis residue and alkali lignin solution, with 10kg water, cleans described alkaline hydrolysis residue, and scavenging solution and described alkali lignin solution merge; Finally obtain 76.41kg alkali lignin solution and 4.69kg alkaline hydrolysis residue (water ratio is 65% left and right); Alkali lignin content in alkali lignin solution is 2.28%, and the extraction yield of alkali lignin is 69%.
Embodiment 3
(1) acid hydrolysis
10.6kg corn cob is smashed, wash dedusting with water, then with 80kg phosphoric acid solution, be hydrolyzed, the mass concentration of phosphoric acid solution is 5%, acid-hydrolyzed temperature is 150 ℃, time is 3 hours, acid hydrolysis residue and pentose solution that rear separation obtains have been hydrolyzed, with 10kg water, clean described acid hydrolysis residue, scavenging solution and described pentose solution merge, (water content is 65% left and right finally to obtain 18.01kg acid hydrolysis residue, the over dry content of hemicellulose is 13.52%, the over dry content of xylogen is 32.49%, cellulosic over dry content is 49%) and 81.98kg pentose solution, the concentration of pentose solution is 3.12%, the extraction yield of hemicellulose is 75%.
(2) cellulase hydrolysis
Get the described acid hydrolysis residue of step (1) as fibrous substrates, carry out cellulase hydrolysis, the condition of described enzymolysis is: cellulase is that above-mentioned penicillin (Penicillium decumbens PD-G3-08) is cultivated the cellulase obtaining, according to
12the cellulosic addition of FPU/g adds cellulase, cellulosic substrate consumption is 80g/L, in temperature, be that 55 ℃, pH are 4.0, under the condition of mixing speed 50rpm, enzymolysis transforms 2 days, whole enzymolysis process, without pressurize, obtains glucose solution, and quality is 85.08kg, concentration is 2.51%, and cellulosic extraction yield is 57%.
(3) alkaline solution extracts alkali lignin
The whole enzymolysis residues that obtain in the present embodiment step (2) are mixed with sodium hydroxide solution, wherein liquid-solid volume ratio is 10: 1, the concentration of sodium hydroxide is 8%, then be warming up to 40 ℃, boiling alkaline hydrolysis through 6 hours, separation obtains alkaline hydrolysis residue and alkali lignin solution, with 10kg water, cleans described alkaline hydrolysis residue, and scavenging solution and described alkali lignin solution merge; Finally obtain 49.05kg alkali lignin solution and 4.78kg alkaline hydrolysis residue (water ratio is 65% left and right); Alkali lignin content in alkali lignin solution is 3.66%, and the extraction yield of alkali lignin is 71%.
Embodiment 4
(1) acid hydrolysis
First by the Wheat Straw that is 11.12kg, (mass component forms: moisture 10.1%, Mierocrystalline cellulose 44%, hemicellulose 22.2%, xylogen 17%, other is 6.7% years old) smash, wash dedusting with water, then with sulphuric acid soln, be hydrolyzed, the mass concentration of sulfuric acid is 0.5%, carrying out acid-hydrolyzed temperature is 140 ℃, time is 2 hours, the acid hydrolysis residue and the pentose solution that obtain, after described acid hydrolysis residue cleans with 10kg water, then scavenging solution and pentose solution merge, (water content is 65% left and right finally to obtain 18.23kg acid hydrolysis residue, the over dry content of hemicellulose is 7.35%, cellulosic over dry content is 58.05%, the over dry content of xylogen is 22.92%) and pentose solution 81.74kg, pentose concentration is 2.45%, the extraction yield of hemicellulose is 81%.
(2) cellulase hydrolysis
Get the described acid hydrolysis residue of step (1) as fibrous substrates, carry out cellulase hydrolysis, the condition of described enzymolysis is: cellulase is that above-mentioned penicillin (Penicillium decumbens PD-G3-08) is cultivated the cellulase obtaining, according to the cellulosic addition of 10FPU/g, add cellulase, cellulosic substrate consumption is 125g/L, in temperature, it is 45 ℃, pH is 6, mixing speed is under the condition of 100rpm, enzymolysis transforms 4 days, whole enzymolysis process is without pressurize, obtain glucose solution, quality is 21.04kg, concentration is 5.56%, cellulosic extraction yield is 58%.
(3) alkaline solution extracts alkali lignin
The present embodiment step (2) is obtained to whole enzymolysis residues mixes with sodium hydroxide solution, according to liquid-solid volume ratio, it is 5: 1, the concentration of sodium hydroxide is 6%, be warming up to 70 ℃, boiling alkaline hydrolysis through 1 hour, separation obtains alkaline hydrolysis residue and alkali lignin solution, with 10kg water, cleans described alkaline hydrolysis residue, and scavenging solution and described alkali lignin solution merge; Finally obtain 6.34kg alkaline hydrolysis residue (water ratio 65% left and right) and 24.91kg alkali lignin solution, in solution, the content of alkali lignin is 5.31%, and the extraction yield of alkali lignin is 70%.
Found through experiments, when acid solution adopts the weak acid that concentration expressed in percentage by weight is 30%, less to xylogen and cellulosic destruction, can realize object of the present invention.And the concentration of phosphoric acid solution is while being 1%, also can realize the present invention, just needed time of acid hydrolysis and temperature of reaction need corresponding increase.
Comparative example 1
Technique and method are with embodiment 1, difference is that step (2) cellulase hydrolysis cellulase used is commercially available cellulase (jade of the He family Bioisystech Co., Ltd, 4w unit, lower same), carry out after cellulase hydrolysis, the quality of glucose solution is 50.2kg, concentration is 2.84%, and cellulosic extraction yield is 38%.
Comparative example 2
Technique and method are with embodiment 2, and difference is that step (2) cellulase hydrolysis cellulase used is commercially available cellulase, carries out after cellulase hydrolysis, and the quality of glucose solution is 44.06kg, and concentration is 3.57%, and cellulosic extraction yield is 42%.
Comparative example 3
Technique and method are with embodiment 4, and difference is that step (2) cellulase hydrolysis cellulase used is commercially available cellulase, carries out after cellulase hydrolysis, and the quality of glucose solution is 51.11kg, and concentration is 3.93%, and cellulosic extraction yield is 41%.
Obviously, above-described embodiment is only for example is clearly described, and the not restriction to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without also giving all embodiments.And the apparent variation of being extended out thus or change are still among the protection domain in the invention.
Claims (9)
1. a method of comprehensive utilization for lignocellulose biomass, is characterized in that comprising the following steps: (a) lignocellulose biomass is carried out to acid hydrolysis, obtain pentose solution and acid hydrolysis residue after separation; (b) use cellulase to carry out enzymolysis to acid hydrolysis residue described in step (a), obtain glucose solution and enzymolysis residue; Described cellulase is for being cultivated the cellulase obtaining by a penicillium, this Penicillium notatum Classification And Nomenclature is
penicilliumdecumbenspD-G3-08, has been preserved in Wuhan University's Chinese Typical Representative culture collection center, and its deposit number is CCTCCM2011195; (c) the described enzymolysis residue producing with alkaline solution treatment step (b), thus the xylogen in described enzymolysis residue extracted.
2. according to the method described in claim 1, it is characterized in that: described acid-hydrolyzed temperature is 100-150 ℃, the time is 0.5-3 hour.
3. according to the method described in claim 2, it is characterized in that: while carrying out described acid hydrolysis, the concentration of acid solution is 0.5-30 % by weight.
4. according to the method described in claim 3, it is characterized in that: described acid solution is phosphoric acid solution, concentration is 1-20 % by weight.
5. according to the method described in any one in claim 1-4, it is characterized in that: the condition of described cellulase hydrolysis is: substrate consumption is 80-150g/L, the addition of cellulase is 10-15FPU/g Mierocrystalline cellulose, temperature is that 45-55 ℃, pH are that 4-6, mixing speed are 50-200rpm, and enzymolysis transformation time is 2-7 days.
6. method according to claim 5, is characterized in that: at alkaline solution described in described step (c), process and carry out at 40-100 ℃.
7. method according to claim 6, is characterized in that: the liquid-solid volume ratio of processing at alkaline solution described in described step (c) is 5:1-20:1.
8. method according to claim 7, is characterized in that: in described alkaline solution processing, the concentration of alkaline solution is 5-8 % by weight.
9. according to the method described in any one in claim 6-8, it is characterized in that: the time that described alkaline solution is processed is 1-6 hour.
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Denomination of invention: Comprehensive utilization method of lignocellulose biomass Effective date of registration: 20160302 Granted publication date: 20140430 Pledgee: Industrial Commercial Bank of China Ltd Zhangqiu branch Pledgor: Shengquan Group Co., Ltd., Ji'nan City Registration number: 2016990000130 |
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Date of cancellation: 20190709 Granted publication date: 20140430 Pledgee: Industrial Commercial Bank of China Ltd Zhangqiu branch Pledgor: Shengquan Group Co., Ltd., Ji'nan City Registration number: 2016990000130 |