CN103044528A - 一种草莓镶脉病毒抗体及其抗原多肽、免疫原和应用 - Google Patents
一种草莓镶脉病毒抗体及其抗原多肽、免疫原和应用 Download PDFInfo
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OD405 | 抗体A-1 | 抗体A-2 | 抗体A-3 | 平均值 | P/N |
多肽P1 | 3.281 | 3.330 | 3.360 | 3.324 | 23.445 |
多肽P2 | 0.158 | 0.125 | 0.123 | 0.135 | 0.954 |
阳性植株(8-1) | 0.781 | 0.749 | 0.900 | 0.810 | 5.712 |
阴性植株(7-5) | 0.215 | 0.186 | 0.197 | 0.199 | 1.406 |
空白 | 0.133 | 0.141 | 0.152 | 0.142 | |
抗体B-1 | 抗体B-2 | 抗体B-3 | |||
多肽P1 | 0.295 | 0.357 | 0.387 | 0.346 | 1.310 |
多肽P2 | 3.220 | 3.331 | 3.144 | 3.232 | 12.231 |
阳性植株(8-1) | 1.076 | 1.037 | 1.080 | 1.064 | 4.029 |
阴性植株(7-5) | 0.265 | 0.283 | 0.297 | 0.282 | 1.066 |
空白 | 0.254 | 0.267 | 0.271 | 0.264 |
OD405 | 重复1 | 重复2 | 重复3 | 平均值 | P/N |
阳性样品 | 1.076 | 1.037 | 1.080 | 1.064 | 7.496 |
阴性样品 | 0.133 | 0.141 | 0.152 | 0.142 | 1.000 |
组培脱毒苗1 | 0.158 | 0.125 | 0.123 | 0.135 | 0.952 |
组培脱毒苗2 | 0.181 | 0.230 | 0.260 | 0.224 | 1.576 |
组培脱毒苗3 | 0.206 | 0.229 | 0.141 | 0.192 | 1.350 |
组培脱毒苗4 | 0.155 | 0.166 | 0.175 | 0.165 | 1.163 |
组培脱毒苗5 | 0.195 | 0.257 | 0.287 | 0.246 | 1.733 |
多代苗1 | 1.237 | 1.234 | 1.276 | 1.249 | 8.793 |
多代苗2 | 1.154 | 1.167 | 1.171 | 1.164 | 8.199 |
多代苗3 | 0.226 | 0.278 | 0.229 | 0.244 | 1.718 |
多代苗4 | 1.302 | 1.281 | 1.206 | 1.263 | 8.894 |
多代苗5 | 0.220 | 0.231 | 0.144 | 0.198 | 1.396 |
多代苗6 | 1.085 | 1.116 | 1.093 | 1.098 | 7.734 |
检测方法 | 阳性样品数 | 阴性样品数 | 总样品数 |
ELISA | 13 | 24 | 37 |
试纸条 | 12 | 25 | 37 |
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CN201310617655.9A CN103739675B (zh) | 2013-01-18 | 2013-01-18 | 草莓镶脉病毒抗体及其抗原多肽、免疫原和应用 |
CN201310020448.5A CN103044528B (zh) | 2013-01-18 | 2013-01-18 | 一种草莓镶脉病毒抗体及其抗原多肽、免疫原和应用 |
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CN114280291A (zh) * | 2021-03-29 | 2022-04-05 | 北京市植物保护站 | 草莓镶脉病毒胶体金试纸条及其制备方法与应用 |
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CN112098654B (zh) * | 2020-09-15 | 2023-07-18 | 武汉生之源生物科技股份有限公司 | 一种胃蛋白酶原i/ii测定试剂盒及其制备方法 |
CN114280292A (zh) * | 2021-03-29 | 2022-04-05 | 北京市植物保护站 | 草莓轻型黄边病毒胶体金试纸条及其制备方法与应用 |
CN113105530A (zh) * | 2021-04-14 | 2021-07-13 | 中国检验检疫科学研究院 | 一种蓝莓休克病毒纳米酶免疫层析试纸条及其制备方法和应用 |
CN113173975A (zh) * | 2021-04-21 | 2021-07-27 | 中国检验检疫科学研究院 | 夜来香花叶病毒纳米酶免疫层析试纸条及其制备方法和应用 |
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WO2002040691A2 (en) * | 2000-11-01 | 2002-05-23 | Maxygen, Inc. | Strawberry vein banding virus (svbv) promoter |
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WO2002040691A2 (en) * | 2000-11-01 | 2002-05-23 | Maxygen, Inc. | Strawberry vein banding virus (svbv) promoter |
Non-Patent Citations (2)
Title |
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陈晓军等: "草莓镶脉病毒检测的一种新方法的研究", 《宁夏大学学报(自然科学版)》, vol. 29, 31 July 2008 (2008-07-31), pages 145 - 150 * |
隋春等: "利用PCR技术检测草莓镶脉病毒", 《园艺学报》, vol. 30, no. 1, 31 December 2003 (2003-12-31), pages 82 - 84 * |
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CN114280291A (zh) * | 2021-03-29 | 2022-04-05 | 北京市植物保护站 | 草莓镶脉病毒胶体金试纸条及其制备方法与应用 |
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