CN103039935B - Process of monosodium glutamate production by rice soaked water - Google Patents
Process of monosodium glutamate production by rice soaked water Download PDFInfo
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- CN103039935B CN103039935B CN2012105442729A CN201210544272A CN103039935B CN 103039935 B CN103039935 B CN 103039935B CN 2012105442729 A CN2012105442729 A CN 2012105442729A CN 201210544272 A CN201210544272 A CN 201210544272A CN 103039935 B CN103039935 B CN 103039935B
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Abstract
The present invention provides a process of monosodium glutamate production by rice soaked water in order to save energy and water and reduce waste water pollution and sewage treatment costs. According to the present invention, the fermentation broth raw material ratio is appropriately adjusted through process innovation, and the rice soaked water is used as a raw material for fermentation directly. Compared with the conventional process, the environmental pressure is reduced, resources are used reasonably, the fermentation acid production yield is improved, and the costs of raw materials are saved.
Description
Technical field
The invention belongs to biological technical field, specifically relate to a kind of technique that is used for glutamate production with rice in steep water replacement corn steep liquor.
Background technology
The slubbing operation of rice sugaring is to clean rice, after General Requirements cleans, changes water 2-3 time repeatedly, enter again next procedure, can produce a large amount of water that soak, its dry matter content accounts for 4%～6%, wherein the main component of bioavailable is glucose, each seed amino acid and trace element, phosphorus, potassium, also contains in addition biology and carries out the essential biotin of vital movement, Cobastab l etc.
All directly to be discharged into environmental protection station to process to live rice in steep water in technique, in recent years, some company is in order to utilize the nutriment in rice washing, improved the rice in steep method, after adopting rice once to add water cleaning, immersion, water is put dried, namely entered next procedure, saved the filtration of repeatedly changing water soaking.Like this, obviously be to have improved the biotin in the hydrolysis sugar liquid and other nutraceutical content, enriched the nutrition of culture medium, it is used for glutamic acid fermentation, make that biotin amount ratio in culture medium is original descends 40%, corn steep liquor reduces to 3% by original 5%, and phosphatic consumption also can suitably reduce, reduce the consumption of auxiliary material, also helped simultaneously the stable of glutamic acid acid production rate.But this technique still can produce part rice in steep water and need to deliver to environmental protection station and process.
, through all kinds of periodical nets and patent network are retrieved, find no the relevant report that rice in steep water is used for glutamate production.
Summary of the invention
, for the deficiencies in the prior art, the purpose of this invention is to provide a kind of rice in steep water and be used for the technique of glutamate production.So that energy savings and water, reduce contaminated wastewater and waste water control expense.The present invention, by technological innovation, suitably adjusts zymotic fluid raw material proportioning,, directly with rice in steep water conservancy use,, as fermentation raw material, replaces the original corn steep liquor that uses and water of part, to reach the purpose of saving water and energy.
Technical scheme of the present invention is as follows: rice in steep water is used for the technique of glutamate production, and its novelty is, with rice in steep water, replaces the corn steep liquor in zymotechnique to prepare fermentation medium, fermenting and producing glutamic acid.
With rice in steep water, inorganic salts, molasses, biotin, rice enzymolysis sugar, phosphoric acid, the bubble enemy, soybean oil, water are mixed with fermentation medium by a certain percentage, carry out sterilizing to connect the mode of disappearing this culture medium, temperature 125-130 degree, time 7-10 minute, be cooled to 33 degree access strain fermentations after sterilizing, extracts after fermentation, refinement treatment is prepared into monosodium glutamate.
Its ratio of described fermentation medium is magnesium sulfate 0.6-0.7g/L, phosphoric acid: 1.5-2.0ml/L, molasses: 5-8g/L, biotin: 10-15ug/L, vitamin B1: 7-10ug/L, rice enzymolysis sugar: 40-50g/L, bubble enemy: 0.4-0.6 g/L, soybean oil: 0.8-1.2 g/L, rice in steep water: 200-250ml/L, pH:7.2-7.4.
Its optimal proportion of described fermentation medium is magnesium sulfate 0.65g/L, phosphoric acid: 1.9ml/L, molasses: 6g/L, biotin: 13ug/L, vitamin B1: 8ug/L, rice enzymolysis sugar: 45g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, rice in steep water: 230ml/L, pH:7.3.
Described rice in steep water is the immersion water after the hanging bag type centrifugal machine removing impurities.
Described rice in steep water is the poly-immersion water that produces of the first step in rice sugaring process, and its process has following step:
(1) the rice puffed wheat tank of packing into, add water, and water overflows and takes away foreign material rice from overfall;
(2) after rice cleaned 1-2 time, Mi Shui drained;
(3) add well water to soak, more than the immersion process water level will exceed rice layer 20cm, the water level timely moisturizing that descends, soak time: summer 0.5-2 hour, winter 3-4 hour;
(4) collecting rice in steep water, to carry out removal of impurities standby.
Rice in steep water by above method preparation contains glucose, each seed amino acid and trace element, phosphorus, potassium, also contains in addition biology and carries out the essential biotin of vital movement, Cobastab l etc.Be prepared culture medium and carry out fermented and cultured by company's self-control formula, it finishes to produce acid and improves, and carries out the extraction of postorder with its tunning, and after refinement treatment, prepared monosodium glutamate meets State Standard of the People's Republic of China GB/T8967-2007 fully.
The invention provides the technique that a kind of rice in steep water is used for glutamate production, this technology utilization need to carry out the rice in steep water of environmental protection treatment originally, belong to the twice laid new technology, this technique is compared and has been reduced environmental protection pressure with former technique, reasonably utilize resource, improve the acid production rate of fermentation, saved expense of raw materials.
The specific embodiment
The invention provides the technique that a kind of rice in steep water is used for glutamate production, below the present invention will be further described for the full embodiment of knot, but do not limit the present invention in any way.
Embodiment 1
Carry out following steps in rice sugaring process and produce immersion water:
(1) the rice puffed wheat tank of packing into, add water, and water overflows and takes away foreign material rice from overfall;
(2) after rice cleaned 1 time, Mi Shui drained;
(3) add well water to soak, more than the immersion process water level will exceed rice layer 20cm, the timely moisturizing of water level decline, soaked 2 hours;
(4) collect rice in steep water, through the hanging bag type centrifugal machine removing impurities.
Be handled as follows take this rice in steep water as raw material:
(1) press magnesium sulfate 0.65g/L, phosphoric acid: 1.9ml/L, molasses: 6g/L, biotin: 13ug/L, vitamin B1: 8ug/L, rice enzymolysis sugar: 45g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, rice in steep water: 230ml/L, pH:7.3, preparation culture medium;
(2) carry out sterilizingly to connect the mode of disappearing this culture medium, temperature 125 degree, 10 minutes time, be cooled to 33 degree access strain fermentations after sterilizing;
(3) extract with existing known technology after fermented and cultured finishes, refinement treatment is prepared into monosodium glutamate.
Embodiment 2
Carry out following steps in rice sugaring process and produce immersion water:
(1) the rice puffed wheat tank of packing into, add water, and water overflows and takes away foreign material rice from overfall;
(2) after rice cleaned 1 time, Mi Shui drained;
(3) add well water to soak, more than the immersion process water level will exceed rice layer 20cm, the timely moisturizing of water level decline, soaked 1.5 hours;
(4) collect rice in steep water, through the hanging bag type centrifugal machine removing impurities.
Be handled as follows take this rice in steep water as raw material:
(1) press magnesium sulfate 0.65g/L, phosphoric acid: 1.9ml/L, molasses: 5g/L, biotin: 13ug/L, vitamin B1: 8ug/L, rice enzymolysis sugar: 45g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, rice in steep water: 250ml/L, pH:7.4, preparation culture medium;
(2) carry out sterilizingly to connect the mode of disappearing this culture medium, temperature 127 degree, 8 minutes time, be cooled to 33 degree access strain fermentations after sterilizing;
(3) extract with existing known technology after fermented and cultured finishes, refinement treatment is prepared into monosodium glutamate.
Embodiment 3
Carry out following steps in rice sugaring process and produce immersion water:
(1) the rice puffed wheat tank of packing into, add water, and water overflows and takes away foreign material rice from overfall;
(2) after rice cleaned 1 time, Mi Shui drained;
(3) add well water to soak, more than the immersion process water level will exceed rice layer 20cm, the timely moisturizing of water level decline, soaked 2.5 hours;
(4) collect rice in steep water, through the hanging bag type centrifugal machine removing impurities.
Be handled as follows take this rice in steep water as raw material:
(1) press magnesium sulfate 0.65g/L, phosphoric acid: 1.9ml/L, molasses: 6g/L, biotin: 13ug/L, vitamin B1: 8ug/L, rice enzymolysis sugar: 45g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, rice in steep water: 200ml/L, pH:7.2, preparation culture medium;
(2) carry out sterilizingly to connect the mode of disappearing this culture medium, temperature 127 degree, 8 minutes time, be cooled to 33 degree access strain fermentations after sterilizing;
(3) extract with existing known technology after fermented and cultured finishes, refinement treatment is prepared into monosodium glutamate.
Embodiment 4
Adopt with embodiment 3 same process parameters and test, difference is that rice in steep water used is 230ml/L.
Embodiment 5
Adopt with embodiment 3 same process parameters and test, difference is that rice in steep water used is 250ml/L.
Respectively carry out 8 batches of experiments by above embodiment 3-5 technique on 260 cubes of fermentation tanks, and with known technology (former technique),, as control group, after fermentation ends, zymotic fluid is detected.
Known technology (former technique): with magnesium sulfate 0.7g/L, phosphoric acid: 2ml/L, molasses: 8g/L, biotin: 14ug/L, vitamin B1: 9ug/L, rice enzymolysis sugar: 50g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, corn steep liquor: 10ml/L, pH:7.2, preparation culture medium; Employing is sterilizing and inoculation with embodiment 3 same way as.
| Embodiment | Batch | Fermentation time | Average external volume | Average product acid |
| 3 | 8 | 28 | 208.2 | 11.21g/dL |
| 4 | 8 | 27.8 | 208.6 | 11.17g/dL |
| 5 | 8 | 27.9 | 208.4 | 11.25g/dL |
| Control group | 8 | 27.9 | 208.3 | 10.75g/dL |
Embodiment 3 first series-produced Pidolidones are detected.
| Index | Industry standard | Control group | Embodiment 3 first |
| Content % (dry weight) | 95 | 95.5 | 96 |
| Optical activity | 31.3 | 30.5 | 30.5 |
| Light transmittance % | 30 | 34 | 36 |
| Sulfate radical % | 0.35 | 0.29 | 0.25 |
The monosodium glutamate of embodiment 3 first series-produced Pidolidones being made with extra care preparation send third party testing agency to detect.
| Project | GB/T8967-2007 index | The present invention |
| Sodium glutamate content, % 〉= | 99.0 | 99.7 |
| Light transmittance, % 〉= | 98 | 99 |
| Chloride is (with CL -Count), %≤ | 0.1 | ＜0.1 |
| PH value | 6.7～7.5 | 7.3 |
| Loss on drying, %≤ | 0.5 | 0.2 |
| Iron, mg/Kg≤ | 5 | ＜5 |
| Sulfate is (with SO 4 2-Count), %≤ | 0.05 | ＜0.05 |
| Arsenic, mg/Kg≤ | 0.5 | ＜0.1 |
| Lead, mg/Kg≤ | 1 | 0.2 |
Learn with rice in steep water and be used for glutamate production according to above many group experiments, replacing corn steep liquor ferments, and carry out the extraction, refining of postorder, the product quality of its production is far above national standard, and can carry high acid, reduce environmental protection pressure, reduce cost of material, can make monosodium glutamate per ton save 500 yuan of costs.
Claims (4)
1. rice in steep water is used for the technique of glutamate production, with rice in steep water, inorganic salts, molasses, biotin, rice enzymolysis sugar, phosphoric acid, bubble enemy, soybean oil, water is mixed with fermentation medium by a certain percentage, with this medium sterilization, and cooling rear inoculation fermentation, extract afterwards, refinement treatment is characterized in that: with rice in steep water, replace the corn steep liquor in technique to prepare fermentation medium, fermenting and producing glutamic acid.
2. rice in steep water described according to claims 1 is used for the technique of glutamate production, it is characterized in that: its ratio of fermentation medium is magnesium sulfate 0.6-0.7g/L, phosphoric acid: 1.5-2.0ml/L, molasses: 5-8g/L, biotin: 10-15ug/L, vitamin B1: 7-10ug/L, rice enzymolysis sugar: 40-50g/L, bubble enemy: 0.4-0.6 g/L, soybean oil: 0.8-1.2 g/L, rice in steep water: 200-250ml/L, pH:7.2-7.4.
3. rice in steep water described according to claims 1 is used for the technique of glutamate production, it is characterized in that: its optimal proportion of fermentation medium is magnesium sulfate 0.65g/L, phosphoric acid: 1.9ml/L, molasses: 6g/L, biotin: 13ug/L, vitamin B1: 8ug/L, rice enzymolysis sugar: 45g/L, bubble enemy: 0.5 g/L, soybean oil: 1 g/L, rice in steep water: 230ml/L, pH:7.3.
4. rice in steep water described according to claims 1 is used for the technique of glutamate production, and it is characterized in that: rice in steep water is the immersion water after the hanging bag type centrifugal machine removing impurities.
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