CN103039754A - Compound feed for improving production performance of laying hen and egg quality - Google Patents
Compound feed for improving production performance of laying hen and egg quality Download PDFInfo
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Abstract
The invention belongs to the technical field of feed, and particularly relates to compound feed for improving the production performance of laying hen and the egg quality. The compound feed consists of corn, lard oil, soybean meal, rape seed cake, peanut meal, gunite bran, mountain flour, calcium hydrophosphate, lysine, methionine, liquid phytase, liquid xylanase and premix. The compound feed provided by the invention can obviously improve the production performance of laying hen and improve the laying rate and the egg quality, and can increase the weight of a single egg and reduce the damage rate of eggs; and moreover, after being eaten by the laying hen, the compound feed can promote digestive absorption, improve the feed conversion rate and the feed-gain ratio, and increase the economic benefits of laying hen farming.
Description
Technical field
The invention belongs to field of feed, particularly a kind of mixed feed that improves performance in layers and egg quality.
Background technology
In recent years, along with the raising of people's living standard, the enhancing of health perception is also strengthened day by day to the requirement of egg quality.Egg quality mainly comprise exterior quality and two sides of interior quality and.Exterior quality mainly is eggshell quality, egg size, egg shape index, and inside quality mainly comprises protein quality, yolk quality, other index.The egg quality that the producers and consumers pays close attention to most is egg size, eggshell quality, yolk color.Although egg presentation quality and egg nutrient relation is little, and is all influential to egg transporting, preservation and consumer's desire to buy, so current attention degree to appearance characters such as eggshell qualities is higher.
Meanwhile, fast development along with animal husbandry, antibiotic is familiar with by people gradually as all drawbacks of feed addictive, when killing harmful germ, also killed the beneficial microbe in the enteron aisle such as it, cause microorganism species imbalance in the enteron aisle, in addition, residual medicine will have a strong impact on treatment and the heredity of human diseases in the livestock products, directly affect human health, the trade barrier of livestock products outlets have also been consisted of simultaneously, bring difficulty for the foreign exchange earning of livestock products, affect to a great extent the economic benefit of aquaculture self.
The additive that is usually used at present improving egg quality mainly contains four classes, is respectively nutrition class additive, plant extracts or Chinese herbal medicine class additive, enzyme preparation class additive and probiotics class additive.
Probiotics has no side effect because it has, safe and reliable, noresidue, the advantage such as free from environmental pollution extremely common people pay close attention to.Some expert's prophesy: " antibiotic of radiance will be brand-new little ecological epoch after the epoch." at present probiotics be the most sure product of substitute antibiotics additive effect, be used for feed and can strengthen immunity of livestock, promote growth and the effective field planting of profitable strain at the laying hen enteron aisle, can also significantly improve egg quality.
Probiotics (Microecological feed additives) refers to can the Direct-fed animal and reach prevent disease by regulating animal intestinal microecology balance, promote growth of animal and improve active microorganism or its culture of efficiency of feed utilization, is called again additive for microbe feedstuff.
Summary of the invention
The object of the present invention is to provide a kind of raising performance in layers of microorganism formulation and mixed feed of egg quality of having added.
The technical solution used in the present invention is as follows:
A kind of mixed feed that improves performance in layers and egg quality, described mixed feed is comprised of following parts by weight of component: corn 59-61 part, lard 0.4-0.6 part, dregs of beans 13-16 part, cotton dregs 1.5-3 part, rape cake 1.5-3.5 part, peanut meal 2-4 part, whitewashing wheat bran 5.5-7.5 part, stone flour 9.1-9.3 part, calcium monohydrogen phosphate 0.5-0.9 part, lysine 0.02-0.07 part, methionine 0.05-0.06 part, liquid phytase 0.02-0.04 part, liquid xylanase 0.03-0.05 part, premix 1-1.5 part.
Preferably, the weight portion of described mixed feed is composed as follows: 60.5 parts of corns, 0.55 part of lard, 13.5 parts of dregs of beans, 2.8 parts of cotton dregs, 3.2 parts of rape cakes, 3 parts of peanut meals, 5.5 parts in whitewashing wheat bran, 9.3 parts of stone flours, 0.5 part of calcium monohydrogen phosphate, 0.04 part of lysine, 0.06 part of methionine, 0.02 part of liquid phytase, 0.03 part of liquid xylanase, 1 part of premix.
The weight portion of described premix is composed as follows: B B-complex 0.015-0.025 part, composite trace element 0.075-0.085 part, bacillus subtilis formulation 0.03-0.05 part, solid-state phytase 0.01-0.02 part, methionine 0.03-0.035 part, salt 0.2-0.4 part, antioxidant 0.004-0.006 part, sodium bicarbonate 0.1-0.2 part, glauber salt 0.1-0.2 part, betaine 0.015-0.017 part, rice bran 0.02-0.4 part.
Preferably, premix is composed as follows: 0.02 part of B B-complex, 0.08 part of composite trace element, 0.04 part of bacillus subtilis formulation, 0.015 part of solid-state phytase, 0.033 part of methionine, 0.3 part of salt, 0.005 part of antioxidant, 0.15 part in sodium bicarbonate, 0.15 part of glauber salt, 0.016 part of betaine, 0.191 part in rice bran.
The crude protein content of described dregs of beans 〉=46%, the crude protein content of described cotton dregs 〉=42%, described rape cake crude protein content 〉=36%, described peanut meal crude protein content 〉=48%, described whitewashing Wheat Brag Protein content 〉=28%, described stone flour calcium content 〉=34.5%, the purity of methionine described in the mixed feed is 99%, and the purity of methionine described in the premix is not less than 84%, described glauber salt sodium sulphate content 〉=99%, solid-state phytase described in the premix, phytase content are 5000U/g.
B B-complex of the present invention, the vitamin premixed feed that can select the Beijing Fuwish Biotechnology Co., Ltd to produce, trade name is FV729, it is as follows that main component is analyzed guarantee value (every kilogram of content): vitamin A 5,000 ten thousand IU, vitamin D
31,500 ten thousand IU, vitamin E 110,000 IU, vitamin K
325g, Cobastab
110 g, Cobastab
230 g, Cobastab
615 g, Cobastab
12100mg, niacinamide 100 g, D-VB5 50 g, folic acid 4 g, Bio 500 mg; Described composite trace element can be selected by Changsha Xingjia Biotechnology Co., Ltd. adding of producing littlely 200, and main component guarantee value (every kilograms of product content) is as follows: iron 100000mg, copper 6500 mg, zinc 55000 mg, manganese 100000 mg, iodine 750 mg, selenium 300mg, cobalt 400 mg.Described bacillus subtilis formulation, the good fortune nation bacillus subtilis (livestock and poultry are special-purpose) that can select Angel Yeast Co.,Ltd to produce, its active constituent content is: bacillus subtilis total viable count 〉=20,000,000,000 CFU/g.
In the above product, also include the carrier rice bran, but carrier is inoperative in the prescription of this mixed feed.
Above raw material all can be bought in market acquisition.
In the feed of the present invention, the action and efficacy of the bacillus subtilis formulation that uses is as follows:
Bacillus subtilis (Dried Bacillus Subtilis Culture, DBSC) is subordinate to the bacterium of Bacillaceae bacillus, and the thalline Grain-positive is shaft-like, single, and size (0.7-0.8) μ m * (2-3) μ m without pod membrane, can move; Give birth in the gemma, cylinder or ellipticity, sporangium is without obviously expanding spore size 0.8 μ m * (1.5-1.8) μ m; Strictly aerobic, bacterium colony is rounded or irregular on nutrient agar, and is opaque, under special circumstances, forms pigment, brown or redness, the orange or black of minority in the bacterium colony or in the contiguous culture medium.The advantages such as that microbial inoculum has is heat-resisting, salt tolerant, high temperature resistant (100 ℃), resist drying.1999 by the official approval use of China Ministry of Agriculture by FDA (FDA) approval use in 1989.This bacterium have bacterial classification single, antibiotic is had no drug resistance and to characteristics such as animal avirulences.Its probiotic properties is: ⑴ is acidproof, salt tolerant, high temperature resistant (100 ℃) and anti-extruding.⑵ do not breed in the small intestine, develop into the auxotype cell with metabolism rapidly at the epimere of enteron aisle, participate in the balance adjustment of flora.⑶ improve the short-chain fat acid content, reduces enteron aisle pH and ammonia concentration.⑷ produce acid, enzyme and multivitamin in the reproductive process.
The thalline of bacillus preparation mainly exists with the form of gemma, bacillus subtilis can the antagonism pathogenic microorganisms, strengthen Immune Function In Animals, simultaneously can produce stronger protease, lipase and amylase, the carbohydrate of the complexity of can effectively degrading, thereby in animal feed, use bacillus subtilis quasi-microorganism additive to have disease preventing and treating, promote the effects such as growth.Research is found, adds the breakage rate that bacillus subtilis formulation can significantly reduce egg in the layer diets, thereby improves the qualification rate of egg, simultaneously to the effect that also improves of egg size, shell thickness, yolk color and hangh unit.
Bird is bacillus subtilis and activating cell wall etc. with the main function composition of bacillus subtilis formulation, play a role by regulating the animal intestine microenvironment, its mechanism that improves performance in layers is: the ⑴ bacillus subtilis is rapid field planting and growth and breeding in animal and bird intestines, consume unnecessary oxygen in the enteron aisle, reduce pH and oxidation-reduction potential, creation is suitable for the environment of physiological anaerobic bacteria existence, promote the useful anaerobic bacteria growths such as Bifidobacterium, recover microecological balance, suppress simultaneously some harmful pathogenic bacteria or conditioned pathogen, the regulating intestinal canal microecological balance, thus make the bacterium colony balance of lacking of proper care because of disease return to the purpose that normal condition reaches treatment and prevention of intestinal tract disease.⑵ bacillus subtilis has stronger protease, amylase and lipase active, can be activated enzyme with activation of zymogen in the body, also can secrete the multiple enzymes such as highly active protease, maltose, lipase, amylase, 1,4 beta-glucanase, phytase and cellulase, some complicated carbohydrate in the energy degrading plant forage, the deficiency of additional animal endogenous enzymes.Bacillus subtilis can be synthesized the multiple nutrients materials such as multivitamin, protein, amino acid, polypeptide, somatomedin in GI metabolic process, improve digestibility and the utilization rate of protein, starch, cellulose, calcium, phosphorus in the feed.
The mechanism that bacillus subtilis is improved egg quality is: the ⑴ bacillus subtilis can produce organic acid or indirectly produce sour environment by promoting lactic acid bacteria to breed in metabolic process, thereby be beneficial to the absorption of various vitamins and mineral element, improve quality of egg.⑵ hen is in the situation of physiological stress, with nutriment from nonessential metabolic activity, such as: the generation of film etc. on the shell then be used for the metabolic activity be badly in need of, the demand of the various nutrients such as vitamin and trace element is all increased, cause forming the relative deficiency of eggshell desired nutritional element.Bacillus subtilis strengthens human body immune function, improves the animal anti-stress ability, reduces the additive decrementation to nutrient.⑶ bacillus subtilis can reduce hen house ammonia mass concentration, improves environment in the hen house.Can slow down breathing on the one hand, make CO
2Discharge capacity reduces, and increases HC0 in the blood
3 -Content is conducive to the formation of eggshell; Also can reduce on the other hand the house in the high concentration ammonia to chicken stress.⑷ bacillus subtilis can be by suppressing the harmful bacteria growths such as Escherichia coli and detection of Salmonella, and the regulating intestinal canal colony balance improves intestinal health, thereby improve body the ability that absorbs of nutrient improved quality of egg.
To sum up, the effect of bacillus subtilis is mainly manifested in: biology is taken oxygen by force, promotes the growth and breeding of useful anaerobe; The antagonism pathogenic microorganisms improves the inside and outside ecological environment; Strengthen immunologic function, promote the resistance against diseases of animal; Promote animal intestinal to grow, strengthen the activity of digestive ferment.In addition, bacillus produces organic acid or by promoting lactic acid bacteria propagation and then producing sour environment, suppresses harmful bacteria in the process of metabolism, promote the absorption of calcium, phosphorus, thereby improve quality of egg.
Beneficial effect of the present invention is:
The invention provides a kind of mixed feed that adds bacillus subtilis formulation, be the novel green feed that a kind of safety has no side effect, described feed can improve performance in layers, improves laying rate and egg production, can improve egg quality, improve an egg size, increase shell thickness, reduce broken rate of eggshell, also can promote to digest and assimilate after laying hen is edible, improve food conversion ratio, improve feedstuff-egg ratio, increase the economic benefit of breeding layer chicken.
The specific embodiment
Below with specific embodiment technical scheme of the present invention is described, but protection scope of the present invention is not limited to this:
Embodiment 1
A kind of mixed feed that improves performance in layers and egg quality, parts by weight of raw materials is composed as follows:
60.5 parts of corns, 0.55 part of lard, 13.5 parts of dregs of beans, 2.8 parts of cotton dregs, 3.2 parts of rape cakes, 3 parts of peanut meals, 5.5 parts in whitewashing wheat bran, 9.3 parts of stone flours, 0.5 part of calcium monohydrogen phosphate, 0.04 part of lysine, 0.06 part of methionine, 0.02 part of liquid phytase, 0.03 part of liquid xylanase, 1 part of premix.Wherein premix is comprised of following component: 0.02 part of B B-complex, 0.08 part of composite trace element, 0.04 part of bacillus subtilis formulation, 0.015 part of solid-state phytase, 0.033 part of methionine, 0.3 part of salt, 0.005 part of antioxidant, 0.15 part in sodium bicarbonate, 0.15 part of glauber salt, 0.016 part of betaine, 0.191 part in rice bran.
Using method: be used for feeding the laying hen of laying period, free choice feeding and drinking-water are fed 3 every day, illumination 16 hours.
Embodiment 2
A kind of mixed feed that improves performance in layers and egg quality, parts by weight of raw materials is composed as follows:
59.5 parts of corns, 0.6 part of lard, 13 parts of dregs of beans, 1.8 parts of cotton dregs, 2.5 parts of rape cakes, 4 parts of peanut meals, 7.5 parts in whitewashing wheat bran, 9.2 parts of stone flours, 0.7 part of calcium monohydrogen phosphate, 0.065 part of lysine, 0.055 part of methionine, 0.035 part of liquid phytase, 0.045 part of liquid xylanase, 1 part of premix.Wherein premix is comprised of following component: 0.025 part of B B-complex, 0.085 part of composite trace element, 0.05 part of bacillus subtilis formulation, 0.02 part of solid-state phytase, 0.035 part of methionine, 0.2 part of salt, 0.006 part of antioxidant, 0.1 part in sodium bicarbonate, 0.1 part of glauber salt, 0.017 part of betaine, 0.362 part in rice bran.
Using method is with embodiment 1.
Embodiment 3
A kind of mixed feed that improves performance in layers and egg quality, parts by weight of raw materials is composed as follows:
61 parts of corns, 0.46 part of lard, 16 parts of dregs of beans, 1.6 parts of cotton dregs, 1.8 parts of rape cakes, 2 parts of peanut meals, 6 parts in whitewashing wheat bran, 9.1 parts of stone flours, 0.9 part of calcium monohydrogen phosphate, 0.02 part of lysine, 0.05 part of methionine, 0.03 part of liquid phytase, 0.04 part of liquid xylanase, 1 part of premix.Wherein premix is comprised of following component: 0.015 part of B B-complex, 0.075 part of composite trace element, 0.03 part of bacillus subtilis formulation, 0.01 part of solid-state phytase, 0.03 part of methionine, 0.4 part of salt, 0.004 part of antioxidant, 0.2 part in sodium bicarbonate, 0.2 part of glauber salt, 0.015 part of betaine, 0.021 part in rice bran.
Using method is with embodiment 1.
The effect example
In order to verify mixed feed of the present invention to the feeding effect of laying hen, carry out by a definite date 45 days feeding experiment in raiser laying hen field.
Single factor contrast test design is taked in test, chooses a healthy laying hen hen house in 1000 48 ages in week, is divided at random test group and control group, and every group comprises 3 repetitions.The test group daily ration adopts embodiment 1 product that has added bacillus subtilis formulation, and the control group daily ration adopts commercially available common mixed feed.Embodiment 1 prescription forms and trophic level sees Table 1.Because two groups of production performances of chicken group own are not in full accord, there is some difference for possibility, accuracy for the guarantee test result, this is tested in earlier stage having carried out first by a definite date 7 days basic data record, afterwards again transition 6 days, data after the transition are the formal data of test, and finally the difference by test data and basic data compares two groups.Duration of test, test chicken adopts three layers of staged to raise in cages, gravity-flow ventilation, free choice feeding and drinking-water, feed every day 3 times, two groups of chicken groups' daily ingestion amount, total egg number, total egg size and contained broken soft egg number are added up in illumination 16 hours, testing crew every day, and observe the searching for food of chicken group, eggshell color, ight soil and other abnormal conditions.
Table 1 test daily ration forms and trophic level
| Daily ration forms (%) | Mass fraction | Trophic level | Mass fraction |
| Corn | 60.5 | Metabolizable energy (MJ/kg) | 2592.85 |
| Lard | 0.55 | Crude protein/% | 16.65 |
| Dregs of beans | 13.5 | Calcium/% | 3.49 |
| Cotton dregs | 2.8 | Total phosphorus/% | 0.46 |
| The dish cake | 3.2 | Available phosphorus/% | 0.24 |
| Peanut meal | 3 | Lysine/% | 0.745 |
| The whitewashing wheat bran | 5.5 | Methionine/% | 0.352 |
| Stone flour | 9.3 | Egg+Guang/% | 0.65 |
| Calcium monohydrogen phosphate | 0.5 | ? | ? |
| Lysine | 0.04 | ? | ? |
| Methionine | 0.06 | ? | ? |
| Liquid phytase | 0.02 | ? | ? |
| Liquid xylanase | 0.03 | ? | ? |
| Premix | 1 | ? | ? |
Result of the test
1) adds bacillus subtilis formulation in the mixed feed to the impact of performance in layers
Can be found out that by table 2 result aspect daily ingestion amount, the amplification contrast group of test group is high by 0.336%; Aspect laying rate, the amplification contrast group of test group is high by 0.707%; Aspect feedstuff-egg ratio, the range of decrease contrast group of test group is high by 4.61%; Aspect death rate, the amplification contrast group of test group is low by 0.22%, illustrates that bacillus subtilis obviously improved performance in layers.
Add bacillus subtilis formulation in table 2 mixed feed to the impact of performance in layers
2) add bacillus subtilis formulation in the mixed feed to the impact of Egg Quality
Can be found out that by table 3 result aspect individual egg size, the amplification contrast group of test group is high by 0.349%; Aspect breakage rate, the range of decrease contrast group of test group is high by 3.6%, illustrates that bacillus subtilis has played certain improvement effect to egg quality.
Add bacillus subtilis formulation in table 3 mixed feed to the impact of Egg Quality
Claims (5)
1. mixed feed that improves performance in layers and egg quality, it is characterized in that, described mixed feed is comprised of following parts by weight of component: corn 59-61 part, lard 0.4-0.6 part, dregs of beans 13-16 part, cotton dregs 1.5-3 part, rape cake 1.5-3.5 part, peanut meal 2-4 part, whitewashing wheat bran 5.5-7.5 part, stone flour 9.1-9.3 part, calcium monohydrogen phosphate 0.5-0.9 part, lysine 0.02-0.07 part, methionine 0.05-0.06 part, liquid phytase 0.02-0.04 part, liquid xylanase 0.03-0.05 part, premix 1-1.5 part.
2. the mixed feed of raising performance in layers as claimed in claim 1 and egg quality, it is characterized in that, the weight portion of described premix is composed as follows: B B-complex 0.015-0.025 part, composite trace element 0.075-0.085 part, bacillus subtilis formulation 0.03-0.05 part, solid-state phytase 0.01-0.02 part, methionine 0.03-0.035 part, salt 0.2-0.4 part, antioxidant 0.004-0.006 part, sodium bicarbonate 0.1-0.2 part, glauber salt 0.1-0.2 part, betaine 0.015-0.017 part, rice bran 0.02-0.4 part.
3. the mixed feed of raising performance in layers as claimed in claim 1 or 2 and egg quality is characterized in that, the weight portion of described mixed feed is composed as follows: 60.5 parts of corns, 0.55 part of lard, 13.5 parts of dregs of beans, 2.8 parts of cotton dregs, 3.2 parts of rape cakes, 3 parts of peanut meals, 5.5 parts in whitewashing wheat bran, 9.3 parts of stone flours, 0.5 part of calcium monohydrogen phosphate, 0.04 part of lysine, 0.06 part of methionine, 0.02 part of liquid phytase, 0.03 part of liquid xylanase, 1 part of premix.
4. the mixed feed of raising performance in layers as claimed in claim 3 and egg quality is characterized in that premix is composed as follows: 0.02 part of B B-complex, 0.08 part of composite trace element, 0.04 part of bacillus subtilis formulation, 0.015 part of solid-state phytase, 0.033 part of methionine, 0.3 part of salt, 0.005 part of antioxidant, 0.15 part in sodium bicarbonate, 0.15 part of glauber salt, 0.016 part of betaine, 0.191 part in rice bran.
5. the mixed feed of raising performance in layers as claimed in claim 3 and egg quality, it is characterized in that, the crude protein content of described dregs of beans 〉=46%, the crude protein content of described cotton dregs 〉=42%, described rape cake crude protein content 〉=36%, described peanut meal crude protein content 〉=48%, described whitewashing Wheat Brag Protein content 〉=28%, described stone flour calcium content 〉=34.5%, the purity of methionine described in the mixed feed is 99%, the purity of methionine described in the premix is not less than 84%, described glauber salt sodium sulphate content 〉=99%, and its phytase content of solid-state phytase described in the premix is 5000U/g.
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| CN103431170A (en) * | 2013-08-19 | 2013-12-11 | 翼城县大众饲料有限公司 | Pollution-free laying hen breeding feed |
| CN103875944A (en) * | 2014-02-24 | 2014-06-25 | 安徽鲲鹏农业科技有限公司 | Formula of feed of laying hens in middle of egg laying period |
| CN104171667B (en) * | 2014-07-30 | 2016-06-29 | 樊继绪 | For the compound chicken feed that laying period laying hen is special |
| CN104171667A (en) * | 2014-07-30 | 2014-12-03 | 樊继绪 | Special composite chicken feed for laying hens in egg producing period |
| CN104304743B (en) * | 2014-09-30 | 2016-10-26 | 柳州市宏华禽蛋养殖有限公司 | A kind of laying hen chicken feed |
| CN104304743A (en) * | 2014-09-30 | 2015-01-28 | 柳州市宏华禽蛋养殖有限公司 | Feed for laying hens |
| CN104304831A (en) * | 2014-11-19 | 2015-01-28 | 浠水县四方饲料有限公司 | Low-phosphorous grain-saving environmentally friendly type laying hen feedstuff |
| CN104905081A (en) * | 2015-07-12 | 2015-09-16 | 宿州市笑果饲料科技有限责任公司 | Egg compound feed |
| CN106472849A (en) * | 2015-08-26 | 2017-03-08 | 河北乐寿农牧集团股份有限公司 | Granular pattern layer chicken in summer special feed and preparation method thereof |
| CN105076898A (en) * | 2015-10-10 | 2015-11-25 | 江苏康威科技饲料有限公司 | Eco-nutritional compound feed for laying hens at egg-laying peak and preparation method of eco-nutritional compound feed |
| CN106666181A (en) * | 2016-03-01 | 2017-05-17 | 青岛普兴生物科技有限公司 | Antibiotic-free, environment-friendly and high-energy layer feed and production technology thereof |
| CN106165783A (en) * | 2016-08-11 | 2016-11-30 | 宿松县春润食品有限公司 | A kind of mixed feed improving Egg Production of Laying Hens quality |
| CN106983039A (en) * | 2017-05-18 | 2017-07-28 | 石家庄广威农牧有限公司 | One breeding hen feed |
| CN106974126A (en) * | 2017-05-19 | 2017-07-25 | 山东省农业科学院家禽研究所 | It is a kind of to reduce egg blackening rate, the feed for improving Egg Quality and preparation method thereof |
| CN107912637A (en) * | 2017-11-27 | 2018-04-17 | 河南世农生物制药有限公司 | A kind of feed addictive for improving laying rate of laying hen and preparation method thereof |
| CN108651758A (en) * | 2018-07-25 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | One kind chicken feed containing phytase and preparation method thereof |
| CN109463558A (en) * | 2018-09-30 | 2019-03-15 | 宁国市农诚农业发展有限公司 | A kind of feed and preparation method thereof of breeding layer chicken prevention disease |
| CN110236019A (en) * | 2019-07-10 | 2019-09-17 | 四川圣迪乐村生态食品股份有限公司 | It is a kind of for producing the egg feedstuff and preparation method thereof of anti-oxidant egg |
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