CN103039713A - Feed additive for improving immunity - Google Patents
Feed additive for improving immunity Download PDFInfo
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- CN103039713A CN103039713A CN2012105018731A CN201210501873A CN103039713A CN 103039713 A CN103039713 A CN 103039713A CN 2012105018731 A CN2012105018731 A CN 2012105018731A CN 201210501873 A CN201210501873 A CN 201210501873A CN 103039713 A CN103039713 A CN 103039713A
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- crude drug
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- feed addictive
- honeysuckle
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Abstract
The invention discloses a feed additive for improving immunity. The feed additive is characterized by comprising the following raw materials: crude drug consisting of pumpkin seed powder, semen plantaginis and honeysuckle, and soil bacteria consisting of bacillus subtilis and thermophilic lactic streptococcus. The feed additive disclosed by the invention can improve the immunity of domestic animal, and can keep the domestic animal healthy without depending on medicines, so as to provide safe edible meat. Compared with the prior art, the feed additive has the advantages that the immunity of the domestic animal can be greatly improved by a small amount of the feed disclosed by the invention, and an excellent effect is achieved.
Description
Technical field
The present invention relates to feed addictive, relate to particularly a kind of feed addictive that improves domestic animal, poultry immunity power.
Background technology
In animal husbandry, all enhance productivity in pursuit all the time.One of them link is taked intensive farm exactly, but along with the increase of stocking density, the deterioration of feeding environment, the health status of domestic animal also descend thereupon.In order to prevent the disease of domestic animals, usually in feed, to add the medicine such as antibiotic.But because the frequent animal pharmaceuticals that use, the drug residue problem of resistance to the action of a drug pathogen and edible meat is also followed generation.So wish by activating the original physiological function of domestic animal, not relying on medicine and just can keep the health of domestic animal, provide the requirement of safe edible meat more and more stronger.
Summary of the invention
The present invention is purpose in line with the feed addictive that efficient, safety is provided and improves immunity.
Technical scheme provided by the invention is as follows:
The inventor finds after by research in order to solve above-mentioned problem, after effectively the enzyme of soil bacteria merges mutually with the crude drug that contains physiologically active, obtains than the achievement of more efficient, safety and raising immunity in the past, thereby finished the present invention.
Technical scheme of the present invention is as follows:
A kind of feed addictive that improves immunity is characterized in that, its raw material comprises:
Crude drug: it comprises the pumpkin powder, plantain seed and honeysuckle;
Soil bacteria: it comprises hay bacillus and Streptomyces thermophilus.
In preferred embodiment of the present invention, after hay bacillus and Streptomyces thermophilus were cultivated in nutrient solution respectively, medium centrifugal was got its supernatant, and this supernatant and crude drug are mixed.
In preferred embodiment of the present invention, in mass ratio, crude drug: pumpkin powder 30-35 part, plantain seed 10-12 part; Honeysuckle 5-6 part.
In preferred embodiment of the present invention, also comprise carrier 55-69 part, described carrier is at least a in rice bran, skin of beancurd and the soybean residue.
In preferred embodiment of the present invention, also comprise at least a in safflower, mineral matter, the vitamin.
A kind of feed addictive that improves immunity is characterized in that, comprising:
Crude drug: pumpkin powder 30-35 part, plantain seed 10-12 part and honeysuckle 5-6 part;
Carrier: rice bran, dregs of beans, soybean residue at least a, 55-69 part;
Soil enzyme: hay bacillus, Streptomyces thermophilus, after the 30-37 degree is cultivated 48-96h, get supernatant dilution 100-300 doubly, then the ratio of 10%-30% is added in crude drug and the carrier in mass ratio.
A kind of preparation method who improves the feed addictive of immunity comprises the steps:
(1) thalline hay bacillus, Streptomyces thermophilus, separate through 40000 centrifuges that turn after cultivating 72 hours at low temperatures respectively 37 ℃ of cultivations, get supernatant; The ratio of the supernatant of two kinds of thalline by volume 1: 0.5-2 is mixed, and with distilled water diluting to 100-200 times of volume, become dilution;
(2) crude drug that uses: pumpkin seeds, plantain seed, honeysuckle are crushed to the 180-200 order; Again and carrier: at least a mixing in defatted rice bran, dregs of beans, the soybean residue; Wherein in mass ratio, pumpkin powder 30-35 part, plantain seed 10-12 part and honeysuckle 5-6 part, carrier 55-69 part; Make compound;
(3) below 50 degree with the compound low temperature drying to moisture below 15%, be ground into after the 3mm granularity for subsequent use.
Pumpkin is the seed of pumpkin, and the normal operation dry product is in order to preserve.It contains multivitamin and pumpkin element.Pumpkin seeds, plantain seed, honeysuckle all can strengthen the effect of interferon (IFN) in the organism-γ.
Streptomyces thermophilus, hay bacillus can be activated the physiological function of domestic animal, excite stomach and intestine physiological regulation function in the carcass,
Carrier itself is the certain trophism of tool also.
Feed addictive through the present invention's exploitation can improve the immunity of domestic animal, does not rely on medicament and just can keep the health of domestic animal, thereby provide safe edible meat.Feed addictive of the present invention only needs to add 0.05%-2% and gets final product in feed, in one embodiment of this invention, only adding 0.1% namely has significant effect.Usefulness compared with the past just can significantly improve the immunity of domestic animal on a small quantity, reaches good effect.The hay bacillus of using and pumpkin seeds etc. also are that the people is edible, are free from side effects fully, can be used as safe feed and use.
Description of drawings
Fig. 1 has shown the measurement result of embodiment three small mouse NK cytoactives, and wherein ordinate is the active ratio of NK cells in mice;
Fig. 2 has shown the measurement result of embodiment three small mouse granulocyte phagocytic functions, and wherein ordinate is the measurement result of granulocyte phagocytic function;
Fig. 3 has shown result's contrast of embodiment four, and wherein ordinate is the death rate (%).
The specific embodiment
The preparation of embodiment one feed addictive
At first, hay bacillus of the present invention, Streptomyces thermophilus are the nonspecific bacterial strains that institute of microbiology preserves, and can buy by commerce to obtain.Pumpkin, plantain seed, honeysuckle also are the article that the non-designated place of production is easy to buy in addition.
Hay bacillus was cultivated through 37 ℃, 72 hours in brain heart leachate broth bouillon (BHI).Under 4 ℃ of conditions, separate through 40000 centrifuges that turn, get supernatant.
Streptomyces thermophilus was cultivated through 37 ℃, 72 hours in Colombia's blood agar culture-medium.Under 4 ℃ of conditions, separate through 40000 centrifuges that turn, get supernatant.
More than two kinds of supernatant equal-volumes mix, with 200 times of distilled water dilutings, become dilution after mixing.
Pumpkin seeds, plantain seed, the honeysuckle of using is the dry thing of moisture content below 15% basically, and mixed proportion is got 30 parts of pumpkin seeds in mass ratio, 10 parts of plantain seeds, 5 parts of honeysuckles, granularity is to be ground into about 200 orders as well, and meticulous words can cause the difficulty that feed mixes.
Pumpkin seeds, plantain seed, honeysuckle mix with 65 portions of defatted rice brans after mixing again, become compound.
Add the dilution of 20% quality in the compound, stir, afterwards in the following low temperature drying of 50 degree to moisture below 15%, save backup as additive after being ground into the granularity about 3mm.
Embodiment two
The reinforcement of domestic animal immunity, the anti-infection property of illness reaches the minimizing of the bacterial load that usually is present in animal.Verified its replace take antibiotics as growth catalyst the function of feed stripped additive.
Add field test effect behind the crude drug in the growth of family chicken as example, (15 farms contrast of 15 farms of added with antibiotic Virginia mycin 20g/ ton and the crude drug goods that add 0.1% pumpkin seeds, plantain seed, honeysuckle in the chicken feed) by 30 farms totally 12 ten thousand chickens test, through 6 time-of-weeks, be that the death rate, body weight, refuse handling rate of chicken is as evaluation criterion with table 1.In the experimental result that table 1 shows, can obviously find out by fed respectively chicken growth parameter(s), raise a family chicken and be can not add antibiotic.
Table 1 is for the effect of family's chicken growing plants property composite enzyme (pumpkin seeds, plantain seed, honeysuckle)
| ? | Add antibiotic feed | Add the feed of crude drug |
| The death rate | 4.5 | 4.3 |
| Age in days | 46.0 | 45.0 |
| Body weight | 5.10 | 5.20 |
| The refuse handling rate | 1.50 | 1.10 |
Embodiment three
Test with the 6 week ages male BALB/c mouse of mouse for buying in from SLC company, raise all rear experiments that is used for.
Each trial flock Classification And Testing size of animal of table 2
| Trial flock | The feed kind | The |
| 1 | |
5 |
| 2 | Dilution+crude drug 0.1% | 5 |
| 3 | Dilution+crude drug 0.5% | 5 |
| 4 | Dilution 0.5% | 5 |
| 5 | Crude drug 0.5% | 5 |
Wherein, the 1st group is the basic feed of nursing, and feeding volume is normal amount.The 2nd group is the additive that adds 0.1% embodiment, 1 preparation on the 1st group basis, the 3rd group is the additive that adds 0.5% embodiment, 1 preparation on the 1st group basis, the 4th group for only adding the dilution among the embodiment one on the 1st group basis, it is only to add crude drug (pumpkin seeds, plantain seed, honeysuckle) on the 1st group basis that addition is 0.5%, the 5 group.
The NK cytoactive is measured the splenocyte effect by observing the target cell death of respectively organizing mouse.As target cell, from the YAC-1 human thymus tumor cells of mouse.For the YAC-1 cell, use in advance red fluorescence dyestuff PKH26 RedFluorescent Cell Linker Kit (Sigma's production) to carry out fluorescence labeling, cell culture fluid (AIM-V, Gibco company makes) is modulated into 2 * 10
5The cell suspension of cells/ml.
At first, the mouse spleen of extraction is made 1 * 10 behind the cell culture fluid washing and filtering
7The cells/ml splenocyte suspension.The YAC-1 cell suspension of this splenocyte suspension and PKH26 mark was mixed into mixed cell suspension by 1: 50 and, was cultivated 4 hours under the 5%CO2 condition at 37 ℃ by the flat culture plate of 96 porocytes (by the manufacturing of Sumitomo Bakelite Co., Ltd).Be convenient control, the YAC-1 cell suspension of red fluorescence dyestuff PKH26 mark is also cultivated by the flat culture plate of 96 porocytes.
Dead cell is after fluorescein TOPRO-3 dyeing in this mixed cell suspension, and we utilize FACSCanto (being produced by BectonDickinson company) flow cytometer to measure the cell mortality of the YAC-1 cell of PKH26 mark.NK cytoactive (percentage) is that the nature cell death rate of deduction YAC-1 cell draws in total cell mortality (%) of the YAC-1 cell that passes through.In addition, total cell mortality of YAC-1 cell is that the cell mortality of this mixed cell suspension, the natural mortality rate of YAC-1 cell are the cell mortalities of being cultivated by the YAC-1 cell suspension of PKH26 mark.
Engulf fluorescently-labeled latex particle by each mouse peripheral blood granulocyte and measure its phagocytosis.At first, in the 1.5ml centrifuge tube, add 1 * 10 of 5 μ L
9The fresh mouse peripheral blood that the fluorescence latex bead solution of beads/mL (Polysciences company product) and 45 μ L take from eye socket.After stirring gently, through 30 minutes 37 ℃ hatch.In addition, in order to destroy red blood cell, the 450 μ L red blood cell solubility solution (ammonium chloride: after 8.29 gram/L, saleratus: 1.0g/L, EDTA2Na:0.037 gram/L) stirs gently, use the granulocytic phagocytic function of FACSCanto cells were tested by flow cytometry peripheral blood that adds ice.
Phagocytic activity is to engulf to draw after the granulocytic survival rate of fluorescence latex bead multiply by the pearl number (beads) that each granulocyte engulfs.
The measurement result of the NK cytoactive that Fig. 1 shows, Fig. 2 has shown the measurement result of granulocyte phagocytic function, among the figure 1～5th of abscissa, is shown in the trial flock of table 2.
From Fig. 1, Fig. 2 can find out clearly that with dilution+pumpkin seeds, plantain seed, the mouse that the mouse that honeysuckle feeds and normal diet are fed relatively can be seen the NK cytoactive, granulocytic phagocytic increasing.Particularly, granulocytic phagocytic activity obviously improves.Therefore, add the pumpkin of dilution, plantain seed, the feed addictive of honeysuckle can effectively improve immunity, Worth Expecting on safety precautions animal infection disease.And only adding 0.1% low concentration amount, on the cytoactive and granulocyte activate the phagocytic capacity that can affect and improve the NK cell, also is very economical.
Can be above-mentioned mixing ferment pumpkin, plantain seed, add again the materials such as safflower, mineral matter, vitamin in honeysuckle and improve effect, also can not produce what side effect.
Embodiment four
1. crude drug: (contain pumpkin, plantain seed, honeysuckle, the preparation method is with embodiment one)
2. dilution: (by hay bacillus, Streptomyces thermophilus preparation, the preparation method is with embodiment one)
3. crude drug+dilution+carrier: contain crude drug (pumpkin, plantain seed, honeysuckle) and soil bacteria (hay bacillus, Streptomyces thermophilus)
Get top Three feed and come raising chicken, chicken death rate in the contrast feeding process
* the kind of chicken: LEGHONG chicken
* quantity: each 100 chicken
The adding rate of feed is 0.3%
Test period: 4 months
As shown in Figure 3, the death rate of the chicken of raising with dilution merely after month is the twice of crude drug+dilution+carrier death rate of supporting.
After four months, the chicken group death rate that crude drug+dilution+vehicle group feed is raised 7.1% during by first month before drops to 3.5%.(dilution group 7.3%, crude drug group 4.2%)
Experiment shows that crude drug+dilution group feed can significantly promote immunity for chickens power, reduces chicken group's the death rate and the incidence of disease that reduces the chicken group.
Claims (8)
1. a feed addictive that improves immunity is characterized in that, its raw material comprises:
Crude drug: it comprises the pumpkin powder, plantain seed and honeysuckle;
Soil bacteria: it comprises hay bacillus and Streptomyces thermophilus.
2. a kind of feed addictive that improves immunity as claimed in claim 1 is characterized in that: after hay bacillus and Streptomyces thermophilus were cultivated in nutrient solution respectively, medium centrifugal was got its supernatant, and this supernatant and crude drug are mixed.
3. a kind of feed addictive that improves immunity as claimed in claim 1 is characterized in that:
In mass ratio, crude drug is composed as follows: pumpkin powder 30-35 part, plantain seed 10-12 part; Honeysuckle 5-6 part.
4. a kind of feed addictive that improves immunity as claimed in claim 3 is characterized in that: also comprise carrier 55-69 part.
5. a kind of feed addictive that improves immunity as claimed in claim 1 is characterized in that: also comprise at least a in safflower, mineral matter, the vitamin.
6. a feed addictive that improves immunity is characterized in that, comprising:
Crude drug: pumpkin powder 30-35 part, plantain seed 10-12 part and honeysuckle 5-6 part;
Carrier: at least a in rice bran, dregs of beans, the soybean residue, 55-69 part;
Soil bacteria: hay bacillus, Streptomyces thermophilus, after the 30-37 degree is cultivated 48-96h, get supernatant dilution 100-300 doubly, then the ratio of 10%-30% is added in crude drug and the carrier in mass ratio.
7. a preparation method who improves the feed addictive of immunity comprises the steps:
(1) thalline hay bacillus, Streptomyces thermophilus, separate through 40000 centrifuges that turn after cultivating 72 hours at low temperatures respectively 37 ℃ of cultivations, get supernatant; The ratio of the supernatant of two kinds of thalline by volume 1: 0.5-2 is mixed, and with distilled water diluting to 100-200 times of volume, become dilution;
(2) crude drug that uses: pumpkin seeds, plantain seed, honeysuckle are crushed to the 180-200 order; Mix with carrier again; Wherein in mass ratio, pumpkin powder 30-35 part, plantain seed 10-12 part and honeysuckle 5-6 part, carrier 55-69 part; Make compound;
(3) below 50 degree with the compound low temperature drying to moisture below 15%, be ground into after the 3mm granularity for subsequent use.
8. a kind of preparation method who improves the feed addictive of immunity as claimed in claim 7 is characterized in that: described carrier is at least a in defatted rice bran, dregs of beans, the soybean residue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105018731A CN103039713A (en) | 2012-11-29 | 2012-11-29 | Feed additive for improving immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105018731A CN103039713A (en) | 2012-11-29 | 2012-11-29 | Feed additive for improving immunity |
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| Publication Number | Publication Date |
|---|---|
| CN103039713A true CN103039713A (en) | 2013-04-17 |
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ID=48052759
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105018731A Pending CN103039713A (en) | 2012-11-29 | 2012-11-29 | Feed additive for improving immunity |
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| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106173728A (en) * | 2016-07-07 | 2016-12-07 | 武汉御花堂绿色科技有限公司 | A kind of enzyme beverage with Fructus Cucurbitae moschatae as main material and preparation method thereof |
| CN109007319A (en) * | 2018-10-13 | 2018-12-18 | 淮北美农生物科技有限公司 | A kind of feed addictive and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150382A (en) * | 1994-06-16 | 1997-05-21 | 山之内制药株式会社 | Feed containing crude drug |
| CN1671404A (en) * | 2002-04-22 | 2005-09-21 | 松浦药业株式会社 | Compositions for preventing or treating pollenosis, allergic nephritis, atopic dermatitits, asthma or urticaria |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
| CN102067940A (en) * | 2010-11-12 | 2011-05-25 | 薛晓宁 | Fish soluble feed protein and processing method thereof |
-
2012
- 2012-11-29 CN CN2012105018731A patent/CN103039713A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150382A (en) * | 1994-06-16 | 1997-05-21 | 山之内制药株式会社 | Feed containing crude drug |
| CN1671404A (en) * | 2002-04-22 | 2005-09-21 | 松浦药业株式会社 | Compositions for preventing or treating pollenosis, allergic nephritis, atopic dermatitits, asthma or urticaria |
| CN101530165A (en) * | 2009-04-23 | 2009-09-16 | 上海谱莱生物技术有限公司 | Animal feed composite microecologic agent |
| CN102067940A (en) * | 2010-11-12 | 2011-05-25 | 薛晓宁 | Fish soluble feed protein and processing method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106173728A (en) * | 2016-07-07 | 2016-12-07 | 武汉御花堂绿色科技有限公司 | A kind of enzyme beverage with Fructus Cucurbitae moschatae as main material and preparation method thereof |
| CN109007319A (en) * | 2018-10-13 | 2018-12-18 | 淮北美农生物科技有限公司 | A kind of feed addictive and preparation method thereof |
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Application publication date: 20130417 |