CN103026876B - Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus - Google Patents
Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus Download PDFInfo
- Publication number
- CN103026876B CN103026876B CN201210561912.7A CN201210561912A CN103026876B CN 103026876 B CN103026876 B CN 103026876B CN 201210561912 A CN201210561912 A CN 201210561912A CN 103026876 B CN103026876 B CN 103026876B
- Authority
- CN
- China
- Prior art keywords
- gram
- ton
- plant
- sulphate
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y02P60/216—
Landscapes
- Fertilizers (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus. The method comprises the following steps of: introduction, tissue cilture, pot culture management, factory management, finished products outplanting and the like. According to the method, the potting media, nutrient solution, leaf fertilizer and the like are optimized; through the method for domesticating the Agastache rugosus, the survival rate can be up to 95%; and delectable pot culture can be cultivated.
Description
Technical field
The invention belongs to a kind of plant cultivation technology, specifically the potted plant domesticating cultivation method of a kind of traditional Chinese medicine wrinkled giant hyssop.
Background technology
Wrinkled giant hyssop is perennial herb, high 30～100 centimetres; That rubs has fragrance.Stem is upright, sturdy, and four prismatics are close by the long pubescence of lark, top multi-branched.Leaf is to life, and wealthy avette, avette or ovum shape ellipse, grows 5～10 centimetres, and wide 1.5～4 centimetres, the leaf of bottom is less, the blunt point of tip, and the wealthy wedge shape of base portion or closely heart-shaped, the irregular crenation of edge tool, two sides is all close by canescence pubescence, and along vein, place is maximum; Long 2～3 centimetres of petiole, close by lark pubescence.The intensive one-tenth spike of wheel umbrella inflorescence, top is raw or armpit is raw, and long 2～8 centimetres, 1～2 centimetre of diameter; Calyx 5 splits, and long 6～8 millimeters of calyx tube is length compared with bract: corolla lip, and lilac red, is about 8 millimeters, and sliver 4 (lower lip 3 splits) is closely isometric, and tip is blunt, full edge; Stamen 4, outstanding, filigree has beard; Ovary is upper, and column cap 2 splits.Nutlet is oval, level and smooth.1～February of florescence.But seldom bloom.Wrinkled giant hyssop, as a kind of medicinal material, adopts seminal propagation (new wrinkled giant hyssop) and perennial root to transplant (old wrinkled giant hyssop) two kinds of propagation methods, plants in large Tanaka, still wrinkled giant hyssop is not tamed for potted plant at present.
Summary of the invention
The potted plant domesticating cultivation method that the object of this invention is to provide a kind of traditional Chinese medicine wrinkled giant hyssop, tames into the potted plant people of confession by wrinkled giant hyssop and views and admires.
Technical scheme of the present invention is as follows:
A potted plant domesticating cultivation method for traditional Chinese medicine wrinkled giant hyssop, is characterized in that comprising the following steps:
One, introduce a fine variety, organize cultivation:
(1), get wrinkled giant hyssop test-tube plantlet blade, band joint stem, not to be with joint stem and the tip of a root be explant, ethanol surface sterilization 1min with 75%, afterwards with 0.1% mercuric chloride sterilization 3min, finally use aseptic water washing 5 times, during sterilization, ceaselessly shake, the modes of emplacement of explant in indefinite bud medium is: blade and the tip of a root keep flat, and band saves stem and is not with joint stem is to insert under morphology lower end; Under aseptic condition, they are inoculated in adventitious bud induction culture base to rear acquisition peak green indefinite bud clump; Adventitious bud induction culture base is: MS+0.5-8.0mg/L 6-BA or 0.3-1 gram/L of 0.1-3.0mg/L TDZ+0.0-1.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(2), peak green indefinite bud clump is moved in corresponding subculture inducing culture and carries out subculture cultivation, grow the healthy and strong seedling that 3-4cm is high; Subculture inducing culture is: 0.3-1 gram/L of MS+0.05-3.0 mg/L TDZ+0.0-0.5mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(3), seedling is transferred in root media, carry out culture of rootage again, the healthy and strong test tube plantlet that grows up to well developed root system, after the hardening of finally uncapping, transplant, root media is: 0.3-1 gram/L of 1/2MS+0.5-2.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0/.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
Wrinkled giant hyssop conditions of tissue culture: temperature keeps (26 ± 1) ℃, illumination every day 12h, intensity of illumination is 2 500～3 000Lx;
Two, potted plant management:
Pogostemon cablin seedling is moved into and is equipped with in the insemination pot of matrix, described matrix is mixed by the raw material of following weight portion: leaf mould 8-10, coarse sand 5-8, Chinese medicine slag 20-30, perlite 10-15, vermiculite 5-8, peat composed of rotten mosses 40-50, after transplanting, in front ten days, within every two days, in matrix, spray one time of nutrition liquid, after ten days, every 3-5 days, spray one time of nutrition liquid, described approval nutrient solution is the aqueous solution, wherein the content of each nutrient component is: nitrate of lime 500-600 gram/ton, potassium nitrate 800-900 gram/ton, ammonium nitrate 50-60 gram/ton, magnesium sulfate 150-200 gram/ton, potassium sulfate 50-60 gram/ton, phosphoric acid 200-250 milliliter/ton, NaFeEDTA 10-20 gram/ton, boric acid 1-3 gram/ton, manganese sulphate 1-3 gram/ton, zinc sulphate 0.1-0.3 gram/ton, copper sulphate 0.05-0.1 gram/ton, ammonium molybdate 0.3-1 gram/ton,
Wrinkled giant hyssop vegetative period, every a foliage fertilizer of spray in 15-20 days, described foliage fertilizer adopts the natural pond liquid after filtered through gauze, every 10 kilograms of natural pond liquid add water 80-120 kilogram, iron ammonium sulfate 3-5 gram and spray, i.e. use after joining, in order to avoid nitrogen volatilization, Radix agastaches obverse and reverse all will spray.
Three, batch production production and administration
The potted plant green house that is placed in of wrinkled giant hyssop is grown;
(1), plant dwarfing technology:
By pinching, pinch, control water, cut root, thereby control plant height, reach the potted plant object of viewing and admiring:
Pinching is mainly to extract the bud point most with apical dominance, and growing tips of the plant is changed, and impels and brings out lateral bud, reaches the object that plant is plentiful, downgrade;
Pinch and refer to that easy spindling plant is too fast because growing, by wiping out a part of top, organize to impel plant nutrition to disperse to supply with;
Control aquatic length when vigorous, control the water yield artificial make plant in semiarid state, reach that plant is short and small, plentiful, side shoot is flourishing;
Cut root by artificially cutting off main root with instrument, control growth;
Four, finished product goes out garden.
The present invention has groped the potted plant domesticating cultivation method of a whole set of traditional Chinese medicine wrinkled giant hyssop, make wrinkled giant hyssop be plant traditional Chinese medicine and transformed potted plant flower, for people, appreciate.
Embodiment
A potted plant domesticating cultivation method for traditional Chinese medicine wrinkled giant hyssop, comprises the following steps:
One, introduce a fine variety, organize cultivation:
(1), get wrinkled giant hyssop test-tube plantlet blade, band joint stem, not to be with joint stem and the tip of a root be explant, ethanol surface sterilization 1min with 75%, afterwards with 0.1% mercuric chloride sterilization 3min, finally use aseptic water washing 5 times, during sterilization, ceaselessly shake, the modes of emplacement of explant in indefinite bud medium is: blade and the tip of a root keep flat, and band saves stem and is not with joint stem is to insert under morphology lower end; Under aseptic condition, they are inoculated in adventitious bud induction culture base to rear acquisition peak green indefinite bud clump; Adventitious bud induction culture base is: MS+0.5-8.0mg/L 6-BA or 0.3-1 gram/L of 0.1-3.0mg/L TDZ+0.0-1.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(2), peak green indefinite bud clump is moved in corresponding subculture inducing culture and carries out subculture cultivation, grow the healthy and strong seedling that 3-4cm is high; Subculture inducing culture is: 0.3-1 gram/L of MS+0.05-3.0 mg/L TDZ+0.0-0.5mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(3), seedling is transferred in root media, carry out culture of rootage again, the healthy and strong test tube plantlet that grows up to well developed root system, after the hardening of finally uncapping, transplant, root media is: 0.3-1 gram/L of 1/2MS+0.5-2.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0/.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
Wrinkled giant hyssop conditions of tissue culture: temperature keeps (26 ± 1) ℃, illumination every day 12h, intensity of illumination is 2 500～3 000Lx;
Two, potted plant management:
Pogostemon cablin seedling is moved into and is equipped with in the insemination pot of matrix, described matrix is mixed by the raw material of following weight portion: leaf mould 8-10, coarse sand 5-8, Chinese medicine slag 20-30, perlite 10-15, vermiculite 5-8, peat composed of rotten mosses 40-50, after transplanting, in front ten days, within every two days, in matrix, spray one time of nutrition liquid, after ten days, every 3-5 days, spray one time of nutrition liquid, described approval nutrient solution is the aqueous solution, wherein the content of each nutrient component is: nitrate of lime 500-600 gram/ton, potassium nitrate 800-900 gram/ton, ammonium nitrate 50-60 gram/ton, magnesium sulfate 150-200 gram/ton, potassium sulfate 50-60 gram/ton, phosphoric acid 200-250 milliliter/ton, NaFeEDTA 10-20 gram/ton, boric acid 1-3 gram/ton, manganese sulphate 1-3 gram/ton, zinc sulphate 0.1-0.3 gram/ton, copper sulphate 0.05-0.1 gram/ton, ammonium molybdate 0.3-1 gram/ton,
Wrinkled giant hyssop vegetative period, every a foliage fertilizer of spray in 15-20 days, described foliage fertilizer adopts the natural pond liquid after filtered through gauze, every 10 kilograms of natural pond liquid add water 80-120 kilogram, iron ammonium sulfate 3-5 gram and spray, i.e. use after joining, in order to avoid nitrogen volatilization, Radix agastaches obverse and reverse all will spray.
Three, batch production production and administration
The potted plant green house that is placed in of wrinkled giant hyssop is grown;
(1), plant dwarfing technology:
By pinching, pinch, control water, cut root, thereby control plant height, reach the potted plant object of viewing and admiring:
Pinching is mainly to extract the bud point most with apical dominance, and growing tips of the plant is changed, and impels and brings out lateral bud, reaches the object that plant is plentiful, downgrade;
Pinch and refer to that easy spindling plant is too fast because growing, by wiping out a part of top, organize to impel plant nutrition to disperse to supply with;
Control aquatic length when vigorous, control the water yield artificial make plant in semiarid state, reach that plant is short and small, plentiful, side shoot is flourishing;
Cut root by artificially cutting off main root with instrument, control growth;
Four, finished product goes out garden.
By above method domestication wrinkled giant hyssop, survival rate reaches 95%, can cultivate and also allow people eyeable potted plant.
Claims (1)
1. a potted plant domesticating cultivation method for traditional Chinese medicine wrinkled giant hyssop, is characterized in that comprising the following steps:
One, introduce a fine variety, organize cultivation:
(1), get wrinkled giant hyssop test-tube plantlet blade, band joint stem, not to be with joint stem and the tip of a root be explant, ethanol surface sterilization 1min with 75%, afterwards with 0.1% mercuric chloride sterilization 3min, finally use aseptic water washing 5 times, during sterilization, ceaselessly shake, the modes of emplacement of explant in indefinite bud medium is: blade and the tip of a root keep flat, and band saves stem and is not with joint stem is to insert under morphology lower end; Under aseptic condition, they are inoculated in adventitious bud induction culture base to rear acquisition peak green indefinite bud clump; Adventitious bud induction culture base is: MS+0.5-8.0mg/L 6-BA or 0.3-1 gram/L of 0.1-3.0mg/L TDZ+0.0-1.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(2), peak green indefinite bud clump is moved in corresponding subculture inducing culture and carries out subculture cultivation, grow the healthy and strong seedling that 3-4cm is high; Subculture inducing culture is: 0.3-1 gram/L of MS+0.05-3.0 mg/L TDZ+0.0-0.5mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
(3), seedling is transferred in root media, carry out culture of rootage again, the healthy and strong test tube plantlet that grows up to well developed root system, after the hardening of finally uncapping, transplant, root media is: 0.3-1 gram/L of 1/2MS+0.5-2.0mg/L IBA+30g/L sucrose+7.5g/L agar+manganese sulphate 1-3 gram/L+ zinc sulphate 0.1-0.3 gram/L+ copper sulphate 0.05-0.1 gram/L+ ammonium molybdate;
Wrinkled giant hyssop conditions of tissue culture: temperature keeps (26 ± 1) ℃, illumination every day 12h, intensity of illumination is 2500～3000Lx;
Two, potted plant management:
Pogostemon cablin seedling is moved into and is equipped with in the insemination pot of matrix, described matrix is mixed by the raw material of following weight portion: leaf mould 8-10, coarse sand 5-8, Chinese medicine slag 20-30, perlite 10-15, vermiculite 5-8, peat composed of rotten mosses 40-50, after transplanting, in front ten days, within every two days, in matrix, spray one time of nutrition liquid, after ten days, every 3-5 days, spray one time of nutrition liquid, described nutrient solution is the aqueous solution, wherein the content of each nutrient component is: nitrate of lime 500-600 gram/ton, potassium nitrate 800-900 gram/ton, ammonium nitrate 50-60 gram/ton, magnesium sulfate 150-200 gram/ton, potassium sulfate 50-60 gram/ton, phosphoric acid 200-250 milliliter/ton, NaFeEDTA 10-20 gram/ton, boric acid 1-3 gram/ton, manganese sulphate 1-3 gram/ton, zinc sulphate 0.1-0.3 gram/ton, copper sulphate 0.05-0.1 gram/ton, ammonium molybdate 0.3-1 gram/ton,
Wrinkled giant hyssop vegetative period, every a foliage fertilizer of spray in 15-20 days, described foliage fertilizer adopts the natural pond liquid after filtered through gauze, every 10 kilograms of natural pond liquid add water 80-120 kilogram, iron ammonium sulfate 3-5 gram and spray, i.e. use after joining, in order to avoid nitrogen volatilization, Radix agastaches obverse and reverse all will spray;
Three, batch production production and administration
The potted plant green house that is placed in of wrinkled giant hyssop is grown;
(1), plant dwarfing technology:
By pinching, pinch, control water, cut root, thereby control plant height, reach the potted plant object of viewing and admiring:
Pinching is mainly to extract the bud point most with apical dominance, and growing tips of the plant is changed, and impels and brings out lateral bud, reaches the object that plant is plentiful, downgrade;
Pinch and refer to that easy spindling plant is too fast because growing, by wiping out a part of top, organize to impel plant nutrition to disperse to supply with;
Control aquatic length when vigorous, control the water yield artificial make plant in semiarid state, reach that plant is short and small, plentiful, side shoot is flourishing;
Cut root by artificially cutting off main root with instrument, control growth;
Four, finished product goes out garden.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210561912.7A CN103026876B (en) | 2012-12-22 | 2012-12-22 | Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210561912.7A CN103026876B (en) | 2012-12-22 | 2012-12-22 | Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103026876A CN103026876A (en) | 2013-04-10 |
| CN103026876B true CN103026876B (en) | 2014-03-26 |
Family
ID=48014706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210561912.7A Active CN103026876B (en) | 2012-12-22 | 2012-12-22 | Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103026876B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041273B (en) * | 2014-06-04 | 2016-08-24 | 李辉 | A kind of potted plant acclimation method of Chinese crude drug Folium Stevlae Rebaudianae |
| CN104094757B (en) * | 2014-07-10 | 2016-03-02 | 李辉 | The potted plant domesticating method of a kind of traditional Chinese medicine leaflet red sandalwood |
| CN106069027B (en) * | 2016-06-16 | 2019-01-29 | 南京中医药大学 | A kind of annual high-efficient method for cultivating of wrinkled giant hyssop potting |
| CN111820083A (en) * | 2019-04-23 | 2020-10-27 | 国药集团同济堂（贵州）制药有限公司 | Seedling growing method of epimedium |
| CN112772332B (en) * | 2021-01-15 | 2022-11-22 | 湖北省农业科学院中药材研究所 | Seedling growing and planting method for codonopsis pilosula in Banqiao mode |
| CN113455303A (en) * | 2021-07-12 | 2021-10-01 | 李辉 | Potting domestication method for traditional Chinese medicinal material chrysanthemum morifolium |
| CN114303945B (en) * | 2021-11-04 | 2023-03-17 | 上海辰山植物园 | In-vitro preparation method of Chinese rose fertile pollen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491531A (en) * | 2003-07-10 | 2004-04-28 | 王德礼 | Method for cultivating mountain edible wild herb and device for storaging and transporting the same |
| CN102703422A (en) * | 2012-06-04 | 2012-10-03 | 广东省中药研究所 | Culture method for new pogostemon cablin breed |
-
2012
- 2012-12-22 CN CN201210561912.7A patent/CN103026876B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491531A (en) * | 2003-07-10 | 2004-04-28 | 王德礼 | Method for cultivating mountain edible wild herb and device for storaging and transporting the same |
| CN102703422A (en) * | 2012-06-04 | 2012-10-03 | 广东省中药研究所 | Culture method for new pogostemon cablin breed |
Non-Patent Citations (8)
| Title |
|---|
| 《广藿香愈伤组织诱导和分化再生植株的研究》;林小桦;《广州中医药大学学报》;20090315;第26卷(第2期);全文 * |
| 《广藿香组培繁殖技术的研究》;刘玉安等;《安徽农业科学》;20091210;第35卷(第37期);全文 * |
| 《广藿香组培苗快繁与工厂化生产程序的研究》;潘超美等;《中药材》;20051125(第11期);全文 * |
| 《广藿香组织培养快繁技术的研究》;曹嵩晓等;《热带生物学报》;20110625;第2卷(第2期);全文 * |
| 刘玉安等.《广藿香组培繁殖技术的研究》.《安徽农业科学》.2009,第35卷(第37期),全文. |
| 曹嵩晓等.《广藿香组织培养快繁技术的研究》.《热带生物学报》.2011,第2卷(第2期),全文. |
| 林小桦.《广藿香愈伤组织诱导和分化再生植株的研究》.《广州中医药大学学报》.2009,第26卷(第2期),全文. |
| 潘超美等.《广藿香组培苗快繁与工厂化生产程序的研究》.《中药材》.2005,(第11期),全文. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103026876A (en) | 2013-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103026876B (en) | Method for domesticating and cultivating pot culture of Chinese herbal medicine Agastache rugosus | |
| CN103404339B (en) | Chinese rose bonsai cultivation method | |
| CN101695283B (en) | Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method | |
| CN104885773B (en) | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling | |
| CN103404336B (en) | Method for using cuttage for seedling raising of colored-leaf plants | |
| CN104041273A (en) | Potting domestication method for traditional Chinese medicine stevia rebaudiana | |
| CN102422810A (en) | In-vitro regeneration culture method for tea clones | |
| CN101491214B (en) | Pinellia tuber artificial seed stem production method | |
| CN107135950A (en) | A kind of breeding method of quick acquisition black fruit fructus lycii regrowth | |
| CN103583358A (en) | Method for in vitro culturing of regenerated plant of dendrobium officinale | |
| CN104705187A (en) | Centranthera grandiflora tissue culture method | |
| CN104041408B (en) | The potted plant domesticating method of a kind of traditional Chinese medicine peppermint | |
| CN104396529A (en) | Cottage reproduction method for shrub althea flower | |
| CN101485290A (en) | Method for establishing rubber tree internal integument regeneration system | |
| CN102017894A (en) | In vitro rapid propagation method of Stephania epigaea | |
| CN103535166A (en) | Lonicera japonica nursery stock rapid propagation method | |
| CN106973796A (en) | A kind of tissue cultivating and seedling method of Idesia polycarpa | |
| CN102090247B (en) | Rapid propagation method of unifying graft and cuttage of camellia | |
| CN103828716A (en) | Tissue culture method of dianthus deltoids | |
| CN105230483A (en) | Method for establishing in-vitro regeneration system of Osmunda vachellii | |
| CN106818476B (en) | The efficient quick propagation method of natural Triploid Lacquer Tree | |
| CN103548695B (en) | A kind of meadowrueleaf corydalis root quick breeding method for tissue culture | |
| CN105724252B (en) | One kind promotes E. dunnii tissue-cultured seedling rooting method | |
| CN105875410B (en) | A kind of rapid propagation method of the hybrid orchid seedling of Chinese cymbidium and Cymbidium hookerianum | |
| CN103858763B (en) | A kind of method that gloxinia tissue culture propagation and stem tuber are preserved and bred |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |