CN103004885B - A kind of brassinosteroid biosynthesis seed coat agent and its production and use - Google Patents
A kind of brassinosteroid biosynthesis seed coat agent and its production and use Download PDFInfo
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- CN103004885B CN103004885B CN201210435042.9A CN201210435042A CN103004885B CN 103004885 B CN103004885 B CN 103004885B CN 201210435042 A CN201210435042 A CN 201210435042A CN 103004885 B CN103004885 B CN 103004885B
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- seed coat
- brassinosteroid biosynthesis
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- coat agent
- biosynthesis
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- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 112
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 title claims abstract description 111
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 title claims abstract description 111
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 167
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 46
- 239000012530 fluid Substances 0.000 claims abstract description 36
- 239000003337 fertilizer Substances 0.000 claims abstract description 32
- 240000002791 Brassica napus Species 0.000 claims abstract description 30
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims abstract description 22
- 244000005700 microbiome Species 0.000 claims abstract description 22
- 239000000243 solution Substances 0.000 claims abstract description 20
- 230000000443 biocontrol Effects 0.000 claims abstract description 18
- 239000003755 preservative agent Substances 0.000 claims abstract description 13
- 230000002335 preservative effect Effects 0.000 claims abstract description 13
- 239000002562 thickening agent Substances 0.000 claims abstract description 13
- 238000004040 coloring Methods 0.000 claims abstract description 12
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 8
- 241000221662 Sclerotinia Species 0.000 claims abstract description 8
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 10
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 8
- 235000010241 potassium sorbate Nutrition 0.000 claims description 8
- 239000004302 potassium sorbate Substances 0.000 claims description 8
- 229940069338 potassium sorbate Drugs 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 7
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 7
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 6
- 229920001285 xanthan gum Polymers 0.000 claims description 6
- 244000215068 Acacia senegal Species 0.000 claims description 5
- 229920000084 Gum arabic Polymers 0.000 claims description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 5
- 239000000205 acacia gum Substances 0.000 claims description 5
- 235000010489 acacia gum Nutrition 0.000 claims description 5
- 229910052748 manganese Inorganic materials 0.000 claims description 5
- 239000011572 manganese Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical group [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 4
- 235000010234 sodium benzoate Nutrition 0.000 claims description 4
- 239000004299 sodium benzoate Substances 0.000 claims description 4
- 229960004025 sodium salicylate Drugs 0.000 claims description 4
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 235000006748 manganese carbonate Nutrition 0.000 claims description 3
- 239000011656 manganese carbonate Substances 0.000 claims description 3
- 229940093474 manganese carbonate Drugs 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 229940099607 manganese chloride Drugs 0.000 claims description 3
- 229910000016 manganese(II) carbonate Inorganic materials 0.000 claims description 3
- XMWCXZJXESXBBY-UHFFFAOYSA-L manganese(ii) carbonate Chemical compound [Mn+2].[O-]C([O-])=O XMWCXZJXESXBBY-UHFFFAOYSA-L 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- SWHAQEYMVUEVNF-UHFFFAOYSA-N magnesium potassium Chemical class [Mg].[K] SWHAQEYMVUEVNF-UHFFFAOYSA-N 0.000 claims description 2
- 229940072033 potash Drugs 0.000 claims description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 2
- 235000015320 potassium carbonate Nutrition 0.000 claims description 2
- 229960002816 potassium chloride Drugs 0.000 claims description 2
- 235000010333 potassium nitrate Nutrition 0.000 claims description 2
- 239000004323 potassium nitrate Substances 0.000 claims description 2
- 229940093914 potassium sulfate Drugs 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 229960003885 sodium benzoate Drugs 0.000 claims description 2
- 230000000857 drug effect Effects 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 239000011248 coating agent Substances 0.000 description 15
- 239000003638 chemical reducing agent Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000003899 bactericide agent Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000006187 pill Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 2
- 239000005906 Imidacloprid Substances 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 2
- 238000012505 colouration Methods 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 2
- 229940056881 imidacloprid Drugs 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 238000005453 pelletization Methods 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
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- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
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- 239000002270 dispersing agent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
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- NYPJDWWKZLNGGM-UHFFFAOYSA-N fenvalerate Chemical compound C=1C=C(Cl)C=CC=1C(C(C)C)C(=O)OC(C#N)C(C=1)=CC=CC=1OC1=CC=CC=C1 NYPJDWWKZLNGGM-UHFFFAOYSA-N 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
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- ATROHALUCMTWTB-OWBHPGMISA-N phoxim Chemical compound CCOP(=S)(OCC)O\N=C(\C#N)C1=CC=CC=C1 ATROHALUCMTWTB-OWBHPGMISA-N 0.000 description 1
- 229950001664 phoxim Drugs 0.000 description 1
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- 244000144977 poultry Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
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- 239000003223 protective agent Substances 0.000 description 1
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- 231100000004 severe toxicity Toxicity 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
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- STCOOQWBFONSKY-UHFFFAOYSA-N tributyl phosphate Chemical compound CCCCOP(=O)(OCCCC)OCCCC STCOOQWBFONSKY-UHFFFAOYSA-N 0.000 description 1
- YNWVFADWVLCOPU-MAUPQMMJSA-N uniconazole P Chemical compound C1=NC=NN1/C([C@@H](O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1 YNWVFADWVLCOPU-MAUPQMMJSA-N 0.000 description 1
- -1 warning colouration Substances 0.000 description 1
- 238000001238 wet grinding Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of brassinosteroid biosynthesis seed coat agent and its production and use.Brassinosteroid biosynthesis seed coat agent of the present invention, with biocontrol microorganisms zymotic fluid for active component, described biocontrol microorganisms is bacillus pumilus (Bacilluspumilus) GBSC66 CGMCC No.3379; Micro-fertilizer, film forming agent solution, thickener, preservative, antifreezing agent, colouring agent can also be comprised in described brassinosteroid biosynthesis seed coat agent.Brassinosteroid biosynthesis seed coat agent of the present invention has that drug effect is high, safety, noresidue, free of contamination advantage, after dressing being carried out to rape seed by brassinosteroid biosynthesis seed coat agent of the present invention, not only growing of rape can be significantly promoted, effectively sclerotinia rot of colza can also be prevented and treated.Meanwhile, brassinosteroid biosynthesis seed coat agent production method of the present invention is simple, with low cost, use conveniently, the storage life is long, is suitable for large-scale industrial production.
Description
Technical field
The present invention relates to a kind of biological pesticide preparation, specifically, is a kind of for brassinosteroid biosynthesis seed coat agent and its production and use.
Background technology
Rape is one of important oil crop of China, and its cultivated area accounts for more than 50% of national oil crop total cultivated area.Along with the increase of cultivated area, rape damage by disease and insect, in ascendant trend year by year, causes yield of rape to lose serious.The seed dressing of severe toxicity, persistent pesticide is the main method of preventing and treating rape seedling damage by disease and insect for many years always, but the Long-Time Service of single chemical pesticide, and damage by disease and insect resistance is increased, and preventive effect reduces.Cause environmental pollution simultaneously, residual serious, even there is person poultry poisoning's event.Efficiently, the research and development of low toxicity, noresidue seed coat agent become problem demanding prompt solution.
Kind of clothing technology is the seed processing technology that Application comparison is general in the world grown up on the basis of tradition seed soaking, seed dressing, is the combination of the standardization of seed quality, the modernization of seeds processing and field integrated pest prevention and control in seedling stage.Seed coat agent is made up of active component and non-active ingredient usually.Active component mainly comprises insecticide, bactericide, hormone, fertilizer or beneficial microbe and metabolite etc. thereof, is the active ingredient directly played a role in seed coat agent.Non-active ingredient refers to film forming agent in seed coat agent and corresponding additive, the physics and the chemical property that maintain seed coat agent, the important component of active component slowly-releasing in controlling diaphragm, comprises film forming agent, dispersant, suspending agent, antifreezing agent, warning colouration, defoamer, thickener, preservative etc.The composition of non-active ingredient directly affects quality and the coating effect of seed coat agent.
CN102197821A discloses a kind of suspension type rape seed coating agent, it is formulated through wet grinding by film forming agent, emulsifier, antifreezing agent, warning colorant and insecticide, bactericide, plant growth regulator, trace element fertilizer and water, is applicable to all kinds of rape seed Cotton seeds.This invention available mechanical method or manual method dressing, pharmacopoeia mass ratio is up to 1:200-300; Can form the clothing film with dense film duct at the surface of the seed during dressing, active component is by fenestra road slow releasing, and the lasting period reaches 55-70 days.
CN1537425A discloses a kind of rape pill pelletizing coating draught-resistant seed coating agent and preparation method thereof, is the white clay of the water-loss reducer of 0.5%-5%, the talcum powder of 1%-10% and 50%-80% is ground into the powder that fineness is more than 200 orders respectively; By 0.15%-0.40% insecticide, 0.35%-0.65% bactericide, 0.05%-0.15%KH
2pO
4, 5%-40% peat and 0.01%-0.03% root-inducing powder Homogeneous phase mixing be ground into the powder that fineness is more than 250 orders; Drop in agitator after water-loss reducer after processing and white clay mix with mixture and stir, obtain rape pill pelletizing coating draught-resistant seed coating agent.
CN 1475117A discloses a kind of coating agent for seed for rape seed.It by auxiliary agent, medicament and water by weight volumn concentration mixing and stirring form.Auxiliary agent is: film forming agent: shitosan, polyvinyl alcohol and gum Arabic; Surfactant: Triton X-100; Antifreezing agent: ethylene glycol; Bleeding agent: acetone; Colloid protective agent: phenol; Defoamer: tributyl phosphate; Warning colouration: red or other coloured pigment; Medicament is: insecticide: insecticides of one or both mixing in Imidacloprid or the same phoxim of Imidacloprid, sumicidin; Bactericide: carbendazim or carbendazim and tmtd; Plant growth regulator: uniconazole P; Trace element: boron fertilizer, manganese fertilizer and zinc fertilizer; Water.
CN 1552177A discloses a kind of rape seed drought-resisting coating pill and pelletized preparation technology thereof.The water-loss reducer solution of configuration 0.05%-1.0%, is sprayed onto water-loss reducer dissolution homogeneity on selected seed, carries out internal layer coating, add clay powder, forms single seed core; Add in seed by draught-resistant seed coating agent again, mixing, while spray into water-loss reducer solution, it is pelletized to carry out middle level.Add and cross 200 object talcum powder on a small quantity, spray into aposematic water-loss reducer solution, carry out outer dressing, after even kind, drying, packaging.
Biological seed coating agent is the important component part of biopesticide, its active component to control the natural products of phytopathogen, living microorganisms or its metabolite, mainly comprises microbiocidal (living microorganism and farm antibiotics etc.), plant source type, take microbial metabolic products as the formulation, genetic engineering bacterial strain etc. of guide's synthesis.Study verified, biological seed coating agent dressing can strengthen seed vitality, improves seedling quality, promotes plant strain growth, improves output, improves crop quality, improve crop resistance.In addition, relative to chemical seed coat agent, biological seed coating agent has safety, noresidue, the advantage such as pollution-free, pays close attention to widely obtaining various countries in recent years.But, have not yet to see the play-by-play about brassinosteroid biosynthesis seed coat agent.
Summary of the invention
One of the object of the invention is for the deficiencies in the prior art, and provide a kind of, low toxicity, safe brassinosteroid biosynthesis seed coat agent nuisanceless to environment, it not only has growth-promoting functions to rape, and effectively can prevent and treat sclerotinia rot of colza.Two of object of the present invention is the preparation method providing described brassinosteroid biosynthesis seed coat agent.Three of object of the present invention is the using method providing described brassinosteroid biosynthesis seed coat agent.Four of object of the present invention is the purposes providing described brassinosteroid biosynthesis seed coat agent.
For one of achieving the above object, the present invention adopts following technical scheme:
A kind of brassinosteroid biosynthesis seed coat agent, its active component is biocontrol microorganisms zymotic fluid, described biocontrol microorganisms is bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, Classification And Nomenclature is bacillus pumilus (Bacillus pumilus) GBSC66, be positioned at the center preservation of China Committee for Culture Collection of Microorganisms of Pekinese common micro-organisms on November 2nd, 2009, preserving number is CGMCC No.3379.
Described bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 encloses soil from the many grass roots of pine in work Bu Jiada town, Tibet in early-stage Study of the present invention to be separated the suitable raw bacterium of the low temperature obtained, be proved the effect having and significantly suppress Sclerotinia sclerotiorum and saccharomycete and remarkable promotion growth of rape, for sclerotinia rot of colza, there is good control efficiency.
Preferably, in described biocontrol microorganisms zymotic fluid, the concentration of biocontrol microorganisms is>=10
7cfu/ml can be such as 10
7cfu/ml, 10
8cfu/ml, 10
9cfu/ml, 10
10cfu/ml, 10
11cfu/ml; More preferably 10
7-10
9cfu/ml; Most preferably be 10
8cfu/ml.
Preferably, the mass percent (wt%) of described biocontrol microorganisms zymotic fluid in brassinosteroid biosynthesis seed coat agent of the present invention is 10wt%-40wt%, such as, can be 10wt%-17.5wt%, 13.6wt%-34wt%, 21.2wt%-37wt%, 29wt%-40wt%, 10wt%, 12wt%, 15wt%, 18.3wt%, 20wt%, 22.5wt%, 25wt%, 28.4wt%, 30wt%, 30.6wt%, 33wt%, 35wt%, 36.1wt%, 39wt%, 40wt%; More preferably 20wt%-30wt%; Most preferably be 25wt%.
Further, micro-fertilizer, film forming agent solution, thickener, antifreezing agent, preservative, colouring agent and water can also be comprised in brassinosteroid biosynthesis seed coat agent of the present invention.
Described micro-fertilizer can provide micro-fertilizer for plant, and such as conventional copper fertilizer, boron fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer and zinc fertilizer etc. can use in the present invention; Be preferably manganese fertilizer and potash fertilizer, such as, have manganese sulphate, manganese carbonate, manganese chloride, potassium chloride, potassium sulfate, potassium dihydrogen phosphate, potassium magnesium salts, potassium nitrate etc.; Most preferably be manganese sulphate and/or potassium chloride.
The mass percent (wt%) of described micro-fertilizer in brassinosteroid biosynthesis seed coat agent of the present invention is 2wt%-5wt%, such as, can be 2wt%-3.5wt%, 2.3wt%-4wt%, 3.2wt%-4.9wt%, 4.1wt%-5wt%, 2wt%, 2.1wt%, 2.5wt%, 2.8wt%, 3wt%, 3.4wt%, 3.6wt%, 3.9wt%, 4wt%, 4.2wt%, 4.5wt%, 4.6wt%, 4.81wt%, 5wt%; More preferably 3wt%-4wt%; Most preferably be 3.5wt%.
The polyvinyl alcohol water solution that described film forming agent solution is preferably the aqueous solution that mass concentration is the sodium carboxymethylcellulose of 3wt%, mass concentration is 4wt%, mass concentration are the mixture of any one or at least two kinds in the chitosan aqueous solution of 2wt%, most preferably are the aqueous solution that mass concentration is the sodium carboxymethylcellulose of 3wt%.
The mass percent (wt%) of described film forming agent solution in brassinosteroid biosynthesis seed coat agent of the present invention is 50wt%-70wt%, such as, can be 50wt%-53.8wt%, 57.3wt%-64wt%, 60.2wt%-65wt%, 59.1wt%-70wt%, 50wt%, 52.1wt%, 53wt%, 54.5wt%, 55wt%, 56wt%, 58.1wt%, 60wt%, 62.8wt%, 63.4wt%, 66wt%, 67.5wt%, 69wt%, 70wt%; More preferably 55wt%-65wt%; Most preferably be 60wt%.
Described thickener is preferably the mixture of any one or at least two kinds in xanthans, gum Arabic and Magnesiumaluminumsilicate, most preferably is xanthans.
The mass percent (wt%) of described thickener in brassinosteroid biosynthesis seed coat agent of the present invention is 0.1wt%-0.5wt%, such as, can be 0.1wt%-0.13wt%, 0.17wt%-0.24wt%, 0.32wt%-0.42wt%, 0.35wt%-0.5wt%, 0.1wt%, 0.14wt%, 0.15wt%, 0.2wt%, 0.21wt%, 0.26wt%, 0.28wt%, 0.3wt%, 0.33wt%, 0.35wt%, 0.37wt%, 0.4wt%, 0.44wt%, 0.45wt%, 0.49wt%, 0.5wt%; More preferably 0.2wt%-0.4wt%; Most preferably be 0.3wt%.
Described antifreezing agent is preferably ethylene glycol and/or propane diols, most preferably is propane diols.
The mass percent (wt%) of described antifreezing agent in brassinosteroid biosynthesis seed coat agent of the present invention is 3wt%-7wt%, such as, can be 3wt%-4.5wt%, 3.9wt%-6wt%, 4.7wt%-5.4wt%, 5.2wt%-7wt%, 3wt%, 3.1wt%, 3.5wt%, 3.92wt%, 4wt%, 4.2wt%, 4.5wt%, 4.83wt%, 5wt%, 5.37wt%, 5.5wt%, 5.8wt%, 6wt%, 6.05wt%, 6.5wt%, 7wt%; More preferably 4wt%-6wt%; Most preferably be 5wt%.
Described preservative is preferably the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate, sodium salicylate, most preferably is potassium sorbate.
The mass percent (wt%) of described preservative in brassinosteroid biosynthesis seed coat agent of the present invention is 0.3wt%-0.7wt%, such as, can be 0.3wt%-0.5wt%, 0.32wt%-0.43wt%, 0.4wt%-0.6wt%, 0.62wt%-0.7wt%, 0.3wt%, 0.33wt%, 0.35wt%, 0.37wt%, 0.4wt%, 0.42wt%, 0.45wt%, 0.48wt%, 0.5wt%, 0.53wt%, 0.55wt%, 0.58wt%, 0.6wt%, 0.65wt%, 0.7wt%; More preferably 0.4wt%-0.6wt%; Most preferably be 0.5wt%.
Described colouring agent is preferably sarranine and/or basic rhodamine, most preferably is basic rhodamine.
The mass percent (wt%) of described colouring agent in brassinosteroid biosynthesis seed coat agent of the present invention is 0.5wt%-1.5wt%, such as, can be 0.5wt%-0.75wt%, 0.62wt%-0.8wt%, 0.7wt%-1.3wt%, 0.92wt%-1.5wt%, 0.5wt%, 0.55wt%, 0.6wt%, 0.63wt%, 0.67wt%, 0.7wt%, 0.75wt%, 0.8wt%, 0.82wt%, 0.9wt%, 0.95wt%, 1wt%, 1.16wt%, 1.25wt%, 1.3wt%, 1.39wt%, 1.4wt%, 1.46wt%, 1.5wt%; More preferably 0.8wt%-1.2wt%; Most preferably be 1wt%.
In sum, the technical scheme after optimization of the present invention is:
A kind of brassinosteroid biosynthesis seed coat agent, comprises following component according to mass percent: zymotic fluid 10wt%-40wt%, micro-fertile 2wt%-5wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, film forming agent solution 50wt%-70wt%, thickener 0.1wt%-0.5wt%, antifreezing agent 3wt%-7wt%, preservative 0.3wt%-0.7wt%, colouring agent 0.5wt%-1.5wt%, all the other are water; In described zymotic fluid, the concentration of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is>=10
7cfu/ml.
Technical scheme after the present invention optimizes further is:
A kind of brassinosteroid biosynthesis seed coat agent, comprises following component according to mass percent: zymotic fluid 20wt%-30wt%, micro-fertile 3wt%-4wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, film forming agent solution 55wt%-65wt%, thickener 0.2wt%-0.4wt%, antifreezing agent 4wt%-6wt%, preservative 0.4wt%-0.6wt%, colouring agent 0.8wt%-1.2wt%, all the other are water; In described zymotic fluid, the concentration of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is 10
7-10
9cfu/ml.
The optimized technical scheme of the present invention is:
A kind of brassinosteroid biosynthesis seed coat agent, comprises following component according to mass percent: zymotic fluid 25wt%, micro-fertile 3.5wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, film forming agent solution 60wt%, thickener 0.3wt%, antifreezing agent 5wt%, preservative 0.5wt%, colouring agent 1wt%, all the other are water; In described zymotic fluid, the concentration of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379 is 10
8cfu/ml.
" comprising " of the present invention, mean it except described component, can also contain other components, these other components give described brassinosteroid biosynthesis seed coat agent with different characteristics.In addition, " comprising " of the present invention, can also replace with enclosed " being " or " by ... make ".No matter which kind of composition is brassinosteroid biosynthesis seed coat agent of the present invention comprise, and the percentage by weight sum of described brassinosteroid biosynthesis seed coat agent is 100%.
For achieve the above object two, the present invention adopts following technical scheme:
A preparation method for brassinosteroid biosynthesis seed coat agent, comprises the steps:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, obtain the satisfactory zymotic fluid of concentration for subsequent use;
(2) zymotic fluid of step (1) gained is mixed according to the ratio in filling a prescription with other component in formula, obtain brassinosteroid biosynthesis seed coat agent of the present invention.
For achieve the above object three, the present invention adopts following technical scheme:
A using method for brassinosteroid biosynthesis seed coat agent carries out dressing by described brassinosteroid biosynthesis seed coat agent to rape seed.
Preferably, the weight ratio of the brassinosteroid biosynthesis seed coat agent that described dressing uses and rape seed is 1:50-1:300, such as, can be 1:50-1:90,1:75-1:110,1:144-1:205,1:190-1:280,1:225-1:300,1:50,1:60,1:88,1:100,1:127,1:150,1:168,1:195,1:203,1:230,1:250,1:264,1:275,1:300; More preferably 1:100-1:200; Most preferably be 1:150.
For achieve the above object four, the present invention adopts following technical scheme:
The application of a kind of brassinosteroid biosynthesis seed coat agent in control sclerotinia rot of colza.
A kind of brassinosteroid biosynthesis seed coat agent is promoting the application in growth of rape.
The technology first Application of biological seed coating agent on rape seed, is had that drug effect is high by brassinosteroid biosynthesis seed coat agent provided by the invention, safety, noresidue, free of contamination advantage, to seed safety, nuisanceless to environment; Particularly physical property is good, and the good and nothing of imbibition comes off, and can play drug effect lastingly.After dressing being carried out to rape seed by brassinosteroid biosynthesis seed coat agent of the present invention, seed vitality can be strengthened, improve resistance, promote plant strain growth, improve output, not only can significantly promote growing of rape, effectively can also prevent and treat sclerotinia rot of colza.Meanwhile, brassinosteroid biosynthesis seed coat agent production method of the present invention is simple, with low cost, use conveniently, the storage life is long, is suitable for large-scale industrial production.
Below in conjunction with embodiment, the present invention is described in further detail.But following embodiment is only simple and easy example of the present invention, does not represent or limits the scope of the present invention, and interest field of the present invention is as the criterion with claims.
Embodiment
For better the present invention being described, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
Embodiment 1:
Be prepared as follows brassinosteroid biosynthesis seed coat agent:
(1) fermented by bacillus pumilus (Bacillus pumilus) GBSC66CGMCC No.3379, obtaining concentration is 10
8the zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following formula: zymotic fluid 30wt%, the manganese sulphate 2wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, potassium chloride 1wt%, mass concentration are aqueous solution 50wt%, the xanthans 0.2wt% of the sodium carboxymethylcellulose of 3wt%, propane diols 5wt%, potassium sorbate 0.3wt%, basic rhodamine 0.5wt%, all the other are water;
(3) other component in being filled a prescription with step (2) by the zymotic fluid of step (1) gained mixes, and obtains brassinosteroid biosynthesis seed coat agent.
During use, the seed coat agent prepared by the present embodiment carries out dressing with the weight ratio of 1:150 to rape seed.
Embodiment 2:
Be prepared as follows brassinosteroid biosynthesis seed coat agent:
(1) fermented by bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, obtaining concentration is 10
7the zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following formula: chitosan aqueous solution 30wt%, gum Arabic 0.4wt% that the polyvinyl alcohol water solution 30wt% that zymotic fluid 25wt%, the manganese carbonate 2wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, potassium sulfate 3wt%, mass concentration are 4wt%, mass concentration are 2wt%, ethylene glycol 3wt%, Sodium Benzoate 0.4wt%, sarranine 0.8wt%, all the other are water;
(3) other component in being filled a prescription with step (2) by the zymotic fluid of step (1) gained mixes, and obtains brassinosteroid biosynthesis seed coat agent.
During use, the seed coat agent prepared by the present embodiment carries out dressing with the weight ratio of 1:50 to rape seed.
Embodiment 3:
Be prepared as follows brassinosteroid biosynthesis seed coat agent:
(1) fermented by bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, obtaining concentration is 10
9the zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following formula: zymotic fluid 40wt%, the potassium dihydrogen phosphate 3.5wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, mass concentration are chitosan aqueous solution 55wt%, the Magnesiumaluminumsilicate 0.1wt% of 2wt%, ethylene glycol 2wt%, propane diols 2wt%, potassium sorbate 0.2wt%, sodium salicylate 0.3wt%, sarranine 0.5wt%, basic rhodamine 0.5wt%, all the other are water;
(3) other component in being filled a prescription with step (2) by the zymotic fluid of step (1) gained mixes, and obtains brassinosteroid biosynthesis seed coat agent.
During use, the seed coat agent prepared by the present embodiment carries out dressing with the weight ratio of 1:300 to rape seed.
Embodiment 4:
Be prepared as follows brassinosteroid biosynthesis seed coat agent:
(1) fermented by bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, obtaining concentration is 10
10the zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following formula: zymotic fluid 20wt%, the manganese chloride 2wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, mass concentration are polyvinyl alcohol water solution 65wt%, the Magnesiumaluminumsilicate 0.3wt% of 4wt%, gum Arabic 0.2wt%, ethylene glycol 6wt%, Sodium Benzoate 0.2wt%, sodium salicylate 0.2wt%, potassium sorbate 0.3wt%, sarranine 1.2wt%, all the other are water;
(3) other component in being filled a prescription with step (2) by the zymotic fluid of step (1) gained mixes, and obtains brassinosteroid biosynthesis seed coat agent.
During use, the seed coat agent prepared by the present embodiment carries out dressing with the weight ratio of 1:200 to rape seed.
Embodiment 5:
Be prepared as follows brassinosteroid biosynthesis seed coat agent:
(1) fermented by bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, obtaining concentration is 10
8the zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following formula: zymotic fluid 10wt%, the potassium chloride 4wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, mass concentration are aqueous solution 70wt%, the xanthans 0.3wt% of the sodium carboxymethylcellulose of 3wt%, propane diols 7wt%, potassium sorbate 0.6wt%, basic rhodamine 1.5wt%, all the other are water;
(3) other component in being filled a prescription with step (2) by the zymotic fluid of step (1) gained mixes, and obtains brassinosteroid biosynthesis seed coat agent.
During use, the seed coat agent prepared by the present embodiment carries out dressing with the weight ratio of 1:100 to rape seed.
Test example:
For investigating brassinosteroid biosynthesis seed coat agent of the present invention in actual applications for the growth-promoting effect of rape and the control efficiency to sclerotinia rot of colza, carry out greenhouse pot culture test as follows:
With the weight ratio of 1:150, dressing is carried out, as experimental group to rape seed by the brassinosteroid biosynthesis seed coat agent that embodiment 1 obtains;
Under the concentration of pesticide-seeds ratio 1:60, dressing is carried out to rape seed, as the first control group with chemical seed coat agent good fortune more than 10% suspension seed-coating agent (Shanghai Shuai Ke Chemical Co., Ltd. provides);
With the process rape seed of clear water as the second control group.
Sow in the usual way after being dried by rape seed after above-mentioned three groups of process and filling sterilized soil and be mixed with in the flowerpot of sclerotinite, after 40 days, observe the incidence of each group of rape and situation of growing, result is as shown in table 1 below:
Table 1
As can be seen from Table 1, brassinosteroid biosynthesis seed coat agent of the present invention does not almost affect for the germination rate of rape, only the germination rate of a little higher than two control groups.And two control groups are all obviously better than in the growth indexes such as plant height, fresh weight, root length, illustrate that brassinosteroid biosynthesis seed coat agent of the present invention effectively can promote growth of rape.The disease index of more each processed group, can find out that the rape incidence of experimental group is the lightest, the control efficiency for sclerotinia rot of colza reaches 61.68%, is obviously better than the first control group of chemical pesticide process.
It should be noted that and understand, when not departing from the spirit and scope of the present invention required by accompanying claim, various amendment and improvement can be made to the present invention of foregoing detailed description.Therefore, the scope of claimed technical scheme is not by the restriction of given any specific exemplary teachings.
Applicant states, above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (38)
1. a brassinosteroid biosynthesis seed coat agent, is characterized in that, described brassinosteroid biosynthesis seed coat agent is become to be grouped into by following according to mass percent:
Biocontrol microorganisms zymotic fluid 10wt% ~ 40wt%, micro-fertile 2wt%-5wt%, film forming agent solution 50wt%-70wt%, thickener 0.1wt%-0.5wt%, antifreezing agent 3wt%-7wt%, preservative 0.3wt%-0.7wt%, colouring agent 0.5wt%-1.5wt%, surplus is water;
Its active component is biocontrol microorganisms zymotic fluid, and described biocontrol microorganisms is bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379;
In described biocontrol microorganisms zymotic fluid, the concentration of biocontrol microorganisms is>=10
7cfu/ml.
2. brassinosteroid biosynthesis seed coat agent according to claim 1, is characterized in that, in described biocontrol microorganisms zymotic fluid, the concentration of biocontrol microorganisms is 10
7-10
9cfu/ml.
3. brassinosteroid biosynthesis seed coat agent according to claim 1, is characterized in that, in described biocontrol microorganisms zymotic fluid, the concentration of biocontrol microorganisms is 10
8cfu/ml.
4. brassinosteroid biosynthesis seed coat agent according to claim 1, is characterized in that, the mass percent of described biocontrol microorganisms zymotic fluid in described brassinosteroid biosynthesis seed coat agent is 20wt%-30wt%.
5. brassinosteroid biosynthesis seed coat agent according to claim 1, is characterized in that, the mass percent of described biocontrol microorganisms zymotic fluid in described brassinosteroid biosynthesis seed coat agent is 25wt%.
6. brassinosteroid biosynthesis seed coat agent according to claim 1, is characterized in that, described micro-fertilizer is copper fertilizer, boron fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer and zinc fertilizer.
7. brassinosteroid biosynthesis seed coat agent according to claim 6, is characterized in that, described micro-fertilizer is manganese fertilizer and potash fertilizer.
8. brassinosteroid biosynthesis seed coat agent according to claim 7, is characterized in that, described micro-fertilizer is manganese sulphate, manganese carbonate, manganese chloride, potassium chloride, potassium sulfate, potassium dihydrogen phosphate, potassium magnesium salts, potassium nitrate.
9. brassinosteroid biosynthesis seed coat agent according to claim 8, is characterized in that, described micro-fertilizer is manganese sulphate and/or potassium chloride.
10. brassinosteroid biosynthesis seed coat agent according to claim 1, it is characterized in that, the aqueous solution of described film forming agent solution to be mass concentration the be sodium carboxymethylcellulose of 3wt%, mass concentration are the polyvinyl alcohol water solution of 4wt%, mixture that mass concentration is any one or at least two kinds in the chitosan aqueous solution of 2wt%.
11. brassinosteroid biosynthesis seed coat agents according to claim 10, is characterized in that, the aqueous solution of described film forming agent solution to be mass concentration the be sodium carboxymethylcellulose of 3wt%.
12. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, described thickener is the mixture of any one or at least two kinds in xanthans, gum Arabic and Magnesiumaluminumsilicate.
13. brassinosteroid biosynthesis seed coat agents according to claim 12, is characterized in that, described thickener is xanthans.
14. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, described antifreezing agent is ethylene glycol and/or propane diols.
15. brassinosteroid biosynthesis seed coat agents according to claim 14, is characterized in that, described antifreezing agent is propane diols.
16. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, described preservative is the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate, sodium salicylate.
17. brassinosteroid biosynthesis seed coat agents according to claim 16, is characterized in that, described preservative is potassium sorbate.
18. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, described colouring agent is sarranine and/or basic rhodamine.
19. brassinosteroid biosynthesis seed coat agents according to claim 18, is characterized in that, described colouring agent is basic rhodamine.
20. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described micro-fertilizer in described brassinosteroid biosynthesis seed coat agent is 3wt%-4wt%.
21. brassinosteroid biosynthesis seed coat agents according to claim 20, is characterized in that, the mass percent of described micro-fertilizer in described brassinosteroid biosynthesis seed coat agent is 3.5wt%.
22. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described film forming agent solution in described brassinosteroid biosynthesis seed coat agent is 55wt%-65wt%.
23. brassinosteroid biosynthesis seed coat agents according to claim 22, is characterized in that, the mass percent of described film forming agent solution in described brassinosteroid biosynthesis seed coat agent is 60wt%.
24. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described thickener in described brassinosteroid biosynthesis seed coat agent is 0.2wt%-0.4wt%.
25. brassinosteroid biosynthesis seed coat agents according to claim 24, is characterized in that, the mass percent of described thickener in described brassinosteroid biosynthesis seed coat agent is 0.3wt%.
26. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described antifreezing agent in described brassinosteroid biosynthesis seed coat agent is 4wt%-6wt%.
27. brassinosteroid biosynthesis seed coat agents according to claim 26, is characterized in that, the mass percent of described antifreezing agent in described brassinosteroid biosynthesis seed coat agent is 5wt%.
28. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described preservative in described brassinosteroid biosynthesis seed coat agent is 0.4wt%-0.6wt%.
29. brassinosteroid biosynthesis seed coat agents according to claim 28, is characterized in that, the mass percent of described preservative in described brassinosteroid biosynthesis seed coat agent is 0.5wt%.
30. brassinosteroid biosynthesis seed coat agents according to claim 1, is characterized in that, the mass percent of described colouring agent in described brassinosteroid biosynthesis seed coat agent is 0.8wt%-1.2wt%.
31. brassinosteroid biosynthesis seed coat agents according to claim 30, is characterized in that, the mass percent of described colouring agent in described brassinosteroid biosynthesis seed coat agent is 1wt%.
32., according to the preparation method of the brassinosteroid biosynthesis seed coat agent one of claim 1-31 Suo Shu, is characterized in that, comprise the steps:
(1) bacillus pumilus (Bacillus pumilus) GBSC66CGMCC No.3379 is fermented, obtain the satisfactory zymotic fluid of concentration for subsequent use;
(2) zymotic fluid of step (1) gained is mixed according to the ratio in filling a prescription with other component in formula, obtain brassinosteroid biosynthesis seed coat agent of the present invention.
33. according to the using method of the brassinosteroid biosynthesis seed coat agent one of claim 1-31 Suo Shu, and it is characterized in that, described method carries out dressing by described brassinosteroid biosynthesis seed coat agent to rape seed.
The using method of 34. brassinosteroid biosynthesis seed coat agents according to claim 33, is characterized in that, the weight ratio of the brassinosteroid biosynthesis seed coat agent that described dressing uses and rape seed is 1:50-1:300.
The using method of 35. brassinosteroid biosynthesis seed coat agents according to claim 33, is characterized in that, the weight ratio of the brassinosteroid biosynthesis seed coat agent that described dressing uses and rape seed is 1:100-1:200.
The using method of 36. brassinosteroid biosynthesis seed coat agents according to claim 33, is characterized in that, the weight ratio of the brassinosteroid biosynthesis seed coat agent that described dressing uses and rape seed is 1:150.
37. according to the application of the brassinosteroid biosynthesis seed coat agent one of claim 1-31 Suo Shu in control sclerotinia rot of colza.
38. are promoting the application in growth of rape according to the brassinosteroid biosynthesis seed coat agent one of claim 1-31 Suo Shu.
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| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| AU2016279764B2 (en) * | 2015-06-16 | 2020-09-10 | Basf Agrochemical Products B.V. | Method for managing flea beetles of the family Chrysomelidae in Brassica crops |
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