CN103004603A - Plant regeneration method for butterfly orchid - Google Patents
Plant regeneration method for butterfly orchid Download PDFInfo
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Abstract
The invention discloses a plant regeneration method for butterfly orchid, belonging to the field of butterfly orchid planting. The plant regeneration method comprises the following steps of: selecting pedicel axillary bud stems with length of 2-5 centimeters on butterfly orchid plants; carrying out induction culture on stem apexes for 2-3 weeks for gradually forming protocorms after 25-30 days; continuously splitting the protocorms to implement subculture acceleration reproduction; moving seedlings with height of one centimeter to a strong seedling culture medium to be cultured for 3-6 months; and inducing the seedlings to root for 20-25 days, and transplanting the strong seedlings with height of 8-10 centimeters, wherein the survive rate of the seedlings is higher than 95%, nursery-grown plants grow well after surviving. The plant regeneration method enables the excellent butterfly orchids to form sterile strains within a short time for implementing large-scale industrial production, can be produced yearly, has a high reproduction speed, and provides novel, fresh, fragrant and colorful flowers with various colors and consistent model. Moreover, a production technology with high technical content is provided to enterprises, so that the plants are consistent in size for facilitating control on flowering phases and uniform management of industrialization, the production costs of the enterprises are reduced, and giant economical benefits are created.
Description
Technical field
The invention belongs to Moth orchid plant quick propagating technology field, be specifically related to a kind of plant regeneration method of butterfly orchid variety.
Background technology
Moth orchid claims again phalaenopsis, belongs to butterfly section Cymbidium, is a kind of perennial single stem epiphytic orchid, originates in the Asia, mainly is distributed in Philippine, the Malay Peninsula and China Taiwan Province.Phalaenopsis is famous cut-flower kind, and its plant type stem attractive in appearance is short, and leaf is large, and scape one is to several pieces, and arch is spent greatly, and lasting its flower shape of florescence such as butterfly with different colors dance in the air, and be beautiful in colour, because flower likeness in form butterfly is gained the name.Its flower appearance is graceful, and color is magnificent, is the treasure in the tropical orchid, the good reputation that " queen in the orchid " arranged, one of extensive, the most universal kind of cultivation in the orchid. whole world initial species approximately has kind more than 70, have more than 530 through the kind of selection cross, but initial species is ornamental relatively poor.Commercial kind for large-scale production mostly is crossbreed, and it is wide in variety, and the florescence is long, and is very popular.The formal name used at school of Moth orchid is by former the meaning of Greek " as the orchid as the butterfly ".It admittedly can absorb airborne nutrient and survive, and is included into the aerial orchid category, can say a big nation in the tropical orchid.Its plant is very peculiar, both without stolon, also without the soccer fraud stem.Every only grows several like broad-leaved plump as the soupspoon, mutual folded being listed on the base portion.The thick aerial root of white then is exposed at around the blade, and what have seeks connections with outer wall at flowerpot, is rich in natural rustic charm.To the time in the new year, one reaches and is full of the bennet of chi and just extracts out from axil, then one to connect a ground open.Every spending all had 5 dusks, and lip is inlayed in the centre.
Its habit of growth happiness high temperature, high humidity, half cloudy environment, the temperature of surviving the winter is not less than 18 degree.Because Moth orchid originates in the tropical rain forest area, the warm chilly of person's character happiness.The growth thermophilic is 15～20 ℃, and will stop growing below the 10C winter, is lower than 5 ℃ of easily death.As producing in batches, cold-proof installation must be arranged in various places, the south of the Five Ridges, carry out the protectiveness cultivation.If family plants in a small amount, just when chance is cold, move into immediately indoor can passing the winter safely.Mostly adopt cellular tissure to cultivate to its breeding, breed seedling replanting through test tube, approximately just can bloom through about 2 years.Some maternal plant is after the florescence finishes, and the axillalry bud on the bennet also can grow sometimes becomes sub-strain, can downcut from bennet when it sends out roots and carry out division propagation.It is potted plant plants material and should not use earth, and will adopt sphagna, float stone, spinulose tree fern bits, charcoal broken etc., perhaps directly seedling is fixed on the vast stupefied plate (claiming again letterwood), allows its voluntarily apposition growth.This cultivation method is to be to copy its ecotope when original.When it is bloomed, the monoblock plank hung on the wall and view and admire, really have a unique lingering charm.The aerial root of Moth orchid is a lot of, and its tip of a root is emerald green, and is quite responsive, carefulness protected, and can't touch damage, otherwise, just look like the face that has broken the people, normally take food just difficult.Moth orchid is liked moist and half cloudy environment, requires the space often to keep humidity 50%～70%, and is can not trickle in the basin too much.If plant in household balcony, preferably spill the wetland face every day three or four times on fine, allow its plant can absorb the moisture that evaporates.Can not allow direct sunlight in summer and autumn.But the Chaoyang in morning is best to its growth, should fully be used.If spring is overcast and rainy too much, will increase illumination to it evening with light pipe, in order to bloom in the future.Moth orchid to the resistance of damage by disease and insect a little less than, often leaf spot and root rot can occur, can adopt Chlorothalonil or reach 1000 times of solution of celestial punching and spray, every spray in seven or eight days 1 time, connect and spray 3 times.These liquids stick in and leave white vestige on the leaf, can erase, and are beneficial to continue the performance bactericidal action.
The pattern of Moth orchid is dazzlingly beautiful, and is existing pure white, light yellow.Stumble red, also have pale purple, the orange red and azure.Have many kinds to have both double-colored or three looks, have as embroidering the striped of pattern, have again just like having sprayed uniform color point, bloom seven or eight for every, many 12, can view and admire continuously six or seven ten days.When all in full bloom, a group butterfly of lining up circles in the air gently seemingly, its sort of elegant not busy feelings, really make us producing a kind of as poem as drawing, like dream unreal sensation seemingly.Wherein to open the comparatively famous and precious of chrysanthemum, a chrysanthemum kind that is called " Mikado " is arranged, can be rated as " superstar ", ask a price very high.Also comparatively rare as for blue flower variety, in the international cattleya fair of nineteen fifty-two and nineteen fifty-three, continuous 2 years trophies of winning the gold medal of the Moth orchid of Taiwan putting on display.The 14th the orchid exhibition that hold in Hong Kong in 1989, the Moth orchid of a red lip of white lobe of Mr.'s HuBing Chi putting on display obtains the main champion prize of the whole audience.Solid foundation has been established in the developing that these outstanding special honours are Moth orchid.Nowadays, European and American countries personage constantly increases the consumption figure of Moth orchid, and senior dinner party all be can't do without Moth orchid and furnished such as.The implication of the Moth orchid representative of various patterns is also different, sulphur butterfly is blue to represent that love is pure, friendship is precious, the red heart Moth orchid represents lucky strike, forever cares, it is smooth and easy, happy happy that red Moth orchid represents official career, bar point Moth orchid representative everything is satisfactory, May all your wish come true, the blue representative cause of the pride of Barbados is flourishing, boom, and mini Moth orchid represents happy angel, at life's full flowering.
Orchid just with plum, bamboo, chrysanthemum and be called four large famous flowers, is likened to " four gentleman " in Ancient Times in China.Queen in the orchid---Moth orchid with the graceful bearing of brilliance, has been confused very the elegant scholars of countless poets especially.For household increases unlimited color and life, improved again the subenvironment air quality simultaneously, absorb poisonous fume, discharge pleasant fresh air, add unlimited dynamic vigor.By blue exhibition, moulded again many people's disposition, make the people refuse between unconsciously dirty dislike dirty, refuse to be contaminated by evil influence, realize again to grow thickly consolidating from its fine bearing, unite to improve oneself, present with all respect unselfishly thereby reach, broadcast fragrance in the well atmosphere in the human world.Gu also often become doting on of great writer great master, be common in writing, spread all generations.Moth orchid also is a kind of " three high product " that has commercial value simultaneously, it has widely medicinal and edibility, but because present people are to the understanding of its research and cultivation, the mystery of orchid plant and value are far from being approved by people, Given this, the present invention passes through the research to plant regeneration and the industrialization development key technology of various pattern butterfly orchid varieties, for the blue industry development of China provides technical support.
Summary of the invention
The object of the present invention is to provide a kind of plant regeneration method of butterfly orchid variety, plant breeding speed is slow in the solution prior art, percentage of seedgermination is low, virus disease is serious, the plant strain growth cycle is inconsistent, flowering time is uneven, in enormous quantities blooming control, directly affect orchid quality and sales volume and price, simultaneously also impact storage and transportation.Institute of the present invention Adopts measure is can make the high-quality butterfly orchid variety set up at short notice aseptic strain to carry out a large amount of industrialization productions, and can produce in the anniversary, reproduction speed is fast, and shades of colour is new, bright, fragrant, the consistent product of gorgeous flower moulding for the consumer provides; For enterprise provides production technology with high content of technology, make its plant in the same size, be convenient to florescence control and industrialization unified management, for reducing enterprise's production cost and providing the product of fine qualities market sales volume of standing firm to bring huge economic benefit.
For achieving the above object, the present invention adopts following technical scheme:
A kind of plant regeneration method of butterfly orchid variety, choose the plant of butterfly orchid variety, cutting length from healthy and strong maternal plant is that 2 ~ 5 centimetres bennet axillary bud stem section is explant material, the cultivation of inducing through 2 ~ 3 weeks of stem apex, it is green that growing point begins to be, cauline leaf begins first growth, then the base portion of growing point begins hypertrophy, formed gradually protocorm in 25 ~ 30 days, the protocorm that forms is by constantly cutting apart, carry out the subculture accelerates multiplication until obtain requirement, cultivated 3 ~ 6 months on the culture vessel strong seedling culture base that the seedling immigration of 1 centimetre of high new formation is larger, length can be transplanted to 8 ~ 10 centimetres high after the root induction in 20～25 days.Specifically may further comprise the steps:
(1) method of drawing material: choose the plant without some pattern butterfly orchid varieties of damage by disease and insect of introducing from Taiwan, cutting length from healthy and strong maternal plant is that 2 ~ 5 centimetres bennet axillary bud stem section is explant material;
(2) preparation of medium:
Bud inducing culture: MS+1.0mg/L BA
+1.0mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+50 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Clump bud inducing culture: 1/2MS+3.0mg/L BA
+0.5mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+150 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Bud proliferated culture medium: 1/2MS+2.0mg/L BA
+0.3g/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+100 g/L bananas juice+1-3 g/L Hyponex1+1-3 g/L Peptone, the pH value is 5.6;
Root media: 1/2 MS+0.5mg/L IBA+0.3mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac, the pH value is 5.6;
(3) material processed: will with lateral bud be cut into the sections of 2 ~ 3cm without some pattern Moth orchid bennets of damage by disease and insect, with the running water flushing, in saturated bleaching powder supernatant, soak 15min again, to constantly stir during immersion; Bennet lateral bud after the immersion drains after 1 hour in lower punching of running water, in superclean bench, after swaying for 3 ~ 5 seconds, the alcohol disinfecting of mass concentration as 75% pour out alcohol, adding mass fraction and be 0.1% mercuric chloride solution processed 8 ~ 12 minutes, pour mercury solution into useless mercury bottle, after sterile water punching 3 ~ 4 times, blot surface moisture with sterilized filter paper;
(4) induce cultivation: the bennet after strict sterilization is cut into 1 section material of 1 bud with scalpel is seeded in the bud inducing culture;
(5) condition of culture: culturing room's temperature is 25 ± 2 ℃, light application time 12h/d, intensity of illumination 1500～2000lx;
(6) the clump bud is induced cultivation: above-mentioned induced bud of inducing cultivation, robust growth is cut into the fritter of 0.5cm, is seeded in respectively from the bud inducing culture; Stem apex begins to expand after 13 ~ 15 days, is light green hemispherical, induces the protocorm caulome after 3 months;
(7) propagation is cultivated: the above-mentioned good protocorm caulome of incubation growth of inducing is cut into fritter, be seeded in respectively bud proliferated culture medium relaying culture; 50 ~ 60 days subcultures once, the propagation multiple is 10 ~ 13, protocorm is placed on the medium that contains the low concentration basic element of cell division to continue to cultivate after 60 days to grow successively budlet, becomes complete protocorm; After 95 days, major part grows up to the seedling of 2 ~ 3 leaves; Bennet sprouting of lateral bud forms seedling, and the blade of recycling seedling is induced the protocorm shoot proliferation, and test-tube plantlet Planting out of test-tube growth traits is stable, and the method by Protocorm Multiplication greatly improves reproduction speed, realizes the industrialization production of Moth orchid;
(8) root induction: when Multiple Buds grows to 2cm when high, bud is divided into individual plant, transfers in the different root medias;
(9) little transplantation of seedlings: to grow to 3～4cm high when test-tube plantlet, 3～5 roots arranged, 5～7 blades, during length 2～3cm, just can carry out hardening, test-tube plantlet was placed on the natural daylight lower refining seedling 5～7 days, open bottle cap and carried out first hardening 2～3 days, to strengthen test-tube plantlet to the adaptive capacity of outdoor environment; Then take out seedling from blake bottle, clean the root medium, plant in the dish of cave with clean water tongue parcel root, keep 25 ~ 30 ℃ of temperature, air humidity is 85%, 25 ~ 30 days, when young leaves, when newly root grows, sprays mass fraction and be 0.3% ~ 0.5% KH
2PO
4Solution, once survival rate reached 95% in per 7 days.
Beneficial effect of the present invention is: the present invention can make the high-quality butterfly orchid variety set up at short notice aseptic strain to carry out a large amount of industrialization productions, and can produce in the anniversary, reproduction speed is fast, and shades of colour is new, bright, fragrant, the consistent product of gorgeous flower moulding for the consumer provides; For enterprise provides production technology with high content of technology, make its plant in the same size, be convenient to florescence control and industrialization unified management, for reducing enterprise's production cost and providing the product of fine qualities market sales volume of standing firm to bring huge economic benefit.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
(1) method of drawing material: choose the plant without some pattern butterfly orchid varieties of damage by disease and insect of introducing from Taiwan, cutting length from healthy and strong maternal plant is that 2 ~ 5 centimetres bennet axillary bud stem section is explant material;
(2) preparation of medium:
Bud inducing culture: MS+1.0mg/L BA
+1.0mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+50 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Clump bud inducing culture: 1/2MS+3.0mg/L BA
+0.5mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+150 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Bud proliferated culture medium: 1/2MS+2.0mg/L BA
+0.3g/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+100 g/L bananas juice+1-3 g/L Hyponex1+1-3 g/L Peptone, the pH value is 5.6;
Root media: 1/2 MS+0.5mg/L IBA+0.3mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac, the pH value is 5.6;
(3) material processed: will with lateral bud be cut into the sections of 2 ~ 3cm without some pattern Moth orchid bennets of damage by disease and insect, with the running water flushing, in saturated bleaching powder supernatant, soak 15min again, to constantly stir during immersion; Bennet lateral bud after the immersion drains after 1 hour in lower punching of running water, in superclean bench, after swaying for 3 ~ 5 seconds, the alcohol disinfecting of mass concentration as 75% pour out alcohol, adding mass fraction and be 0.1% mercuric chloride solution processed 8 ~ 12 minutes, pour mercury solution into useless mercury bottle, after sterile water punching 3 ~ 4 times, blot surface moisture with sterilized filter paper;
(4) induce cultivation: the bennet after strict sterilization is cut into 1 section material of 1 bud with scalpel is seeded in the bud inducing culture;
(5) condition of culture: culturing room's temperature is 25 ± 2 ℃, light application time 12h/d, intensity of illumination 1500～2000lx;
(6) the clump bud is induced cultivation: above-mentioned induced bud of inducing cultivation, robust growth is cut into the fritter of 0.5cm, is seeded in respectively from the bud inducing culture; Stem apex begins to expand after 13 ~ 15 days, is light green hemispherical, induces the protocorm caulome after 3 months;
(7) propagation is cultivated: the above-mentioned good protocorm caulome of incubation growth of inducing is cut into fritter, be seeded in respectively bud proliferated culture medium relaying culture; 50 ~ 60 days subcultures once, the propagation multiple is 10 ~ 13, protocorm is placed on the medium that contains the low concentration basic element of cell division to continue to cultivate after 60 days to grow successively budlet, becomes complete protocorm; After 95 days, major part grows up to the seedling of 2 ~ 3 leaves; Bennet sprouting of lateral bud forms seedling, and the blade of recycling seedling is induced the protocorm shoot proliferation, and test-tube plantlet Planting out of test-tube growth traits is stable, and the method by Protocorm Multiplication greatly improves reproduction speed, realizes the industrialization production of Moth orchid;
(8) root induction: when Multiple Buds grows to 2cm when high, bud is divided into individual plant, transfers in the different root medias;
(9) little transplantation of seedlings: to grow to 3～4cm high when test-tube plantlet, 3～5 roots arranged, 5～7 blades, during length 2～3cm, just can carry out hardening, test-tube plantlet was placed on the natural daylight lower refining seedling 5～7 days, open bottle cap and carried out first hardening 2～3 days, to strengthen test-tube plantlet to the adaptive capacity of outdoor environment; Then take out seedling from blake bottle, clean the root medium, plant in the dish of cave with clean water tongue parcel root, keep 25 ~ 30 ℃ of temperature, air humidity is 85%, 25 ~ 30 days, when young leaves, when newly root grows, sprays mass fraction and be 0.3% ~ 0.5% KH
2PO
4Solution, once survival rate reached 95% in per 7 days.
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. the plant regeneration method of a butterfly orchid variety, it is characterized in that: the plant of choosing butterfly orchid variety, cutting length from healthy and strong maternal plant is that 2 ~ 5 centimetres bennet axillary bud stem section is explant material, the cultivation of inducing through 2 ~ 3 weeks of stem apex, it is green that growing point begins to be, cauline leaf begins first growth, then the base portion of growing point begins hypertrophy, formed gradually protocorm in 25 ~ 30 days, the protocorm that forms is by constantly cutting apart, carry out the subculture accelerates multiplication until obtain requirement, cultivated 3 ~ 6 months on the culture vessel strong seedling culture base that the seedling immigration of 1 centimetre of high new formation is larger, length can be transplanted to 8 ~ 10 centimetres high after the root induction in 20～25 days.
2. the plant regeneration method of butterfly orchid variety according to claim 1 is characterized in that: may further comprise the steps:
(1) method of drawing material: choose the plant without some pattern butterfly orchid varieties of damage by disease and insect of introducing from Taiwan, cutting length from healthy and strong maternal plant is that 2 ~ 5 centimetres bennet axillary bud stem section is explant material;
(2) preparation of medium:
Bud inducing culture: MS+1.0mg/L BA
+1.0mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+50 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Clump bud inducing culture: 1/2MS+3.0mg/L BA
+0.5mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+150 g/L coconut milk+1-3 g/L Peptone, the pH value is 5.6;
Bud proliferated culture medium: 1/2MS+2.0mg/L BA
+0.3g/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac+100 g/L bananas juice+1-3 g/L Hyponex1+1-3 g/L Peptone, the pH value is 5.6;
Root media: 1/2 MS+0.5mg/L IBA+0.3mg/L NAA+6.5 g/L Ag+30 g/L Su+2-2.5 g/L Ac, the pH value is 5.6;
(3) material processed: will with lateral bud be cut into the sections of 2 ~ 3cm without some pattern Moth orchid bennets of damage by disease and insect, with the running water flushing, in saturated bleaching powder supernatant, soak 15min again, to constantly stir during immersion; Bennet lateral bud after the immersion drains after 1 hour in lower punching of running water, in superclean bench, after swaying for 3 ~ 5 seconds, the alcohol disinfecting of mass concentration as 75% pour out alcohol, adding mass fraction and be 0.1% mercuric chloride solution processed 8 ~ 12 minutes, pour mercury solution into useless mercury bottle, after sterile water punching 3 ~ 4 times, blot surface moisture with sterilized filter paper;
(4) induce cultivation: the bennet after strict sterilization is cut into 1 section material of 1 bud with scalpel is seeded in the bud inducing culture;
(5) condition of culture: culturing room's temperature is 25 ± 2 ℃, light application time 12h/d, intensity of illumination 1500～2000lx;
(6) the clump bud is induced cultivation: above-mentioned induced bud of inducing cultivation, robust growth is cut into the fritter of 0.5cm, is seeded in respectively from the bud inducing culture; Stem apex begins to expand after 13 ~ 15 days, is light green hemispherical, induces the protocorm caulome after 3 months;
(7) propagation is cultivated: the above-mentioned good protocorm caulome of incubation growth of inducing is cut into fritter, be seeded in respectively bud proliferated culture medium relaying culture; 50 ~ 60 days subcultures once, the propagation multiple is 10 ~ 13, protocorm is placed on the medium that contains the low concentration basic element of cell division to continue to cultivate after 60 days to grow successively budlet, becomes complete protocorm; After 95 days, major part grows up to the seedling of 2 ~ 3 leaves; Bennet sprouting of lateral bud forms seedling, and the blade of recycling seedling is induced the protocorm shoot proliferation, and test-tube plantlet Planting out of test-tube growth traits is stable, and the method by Protocorm Multiplication greatly improves reproduction speed, realizes the industrialization production of Moth orchid;
(8) root induction: when Multiple Buds grows to 2cm when high, bud is divided into individual plant, transfers in the different root medias;
(9) little transplantation of seedlings: to grow to 3～4cm high when test-tube plantlet, 3～5 roots arranged, 5～7 blades, during length 2～3cm, just can carry out hardening, test-tube plantlet was placed on the natural daylight lower refining seedling 5～7 days, open bottle cap and carried out first hardening 2～3 days, to strengthen test-tube plantlet to the adaptive capacity of outdoor environment; Then take out seedling from blake bottle, clean the root medium, plant in the dish of cave with clean water tongue parcel root, keep 25 ~ 30 ℃ of temperature, air humidity is 85%, 25 ~ 30 days, when young leaves, when newly root grows, sprays mass fraction and be 0.3% ~ 0.5% KH
2PO
4Solution, once survival rate reached 95% in per 7 days.
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| CN103782911A (en) * | 2014-02-07 | 2014-05-14 | 福建省亚热带植物研究所 | Regulating and controlling method for synchronization of somatic embryo of butterfly orchid |
| CN103798136A (en) * | 2013-12-11 | 2014-05-21 | 柳州赛特生物科技研发中心 | Special phalaenopsis tissue culture medium |
| CN103843567A (en) * | 2014-03-10 | 2014-06-11 | 上海市农业科学院 | Method for rapidly propagating Chinese cymbidium by using root induced protocorm and culture medium thereof |
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| CN104082148A (en) * | 2014-07-18 | 2014-10-08 | 内蒙古农业大学 | Method for performing regeneration propagation on sterile stalks of butterfly orchids |
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| CN114287344A (en) * | 2022-01-07 | 2022-04-08 | 赵英 | Tissue culture medium of phalaenopsis and propagation method thereof |
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