CN102998288A - Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water - Google Patents
Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water Download PDFInfo
- Publication number
- CN102998288A CN102998288A CN2012103643066A CN201210364306A CN102998288A CN 102998288 A CN102998288 A CN 102998288A CN 2012103643066 A CN2012103643066 A CN 2012103643066A CN 201210364306 A CN201210364306 A CN 201210364306A CN 102998288 A CN102998288 A CN 102998288A
- Authority
- CN
- China
- Prior art keywords
- iii
- rayleigh scattering
- solution
- aussdna
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water. The aptamer-nanometer gold syntony Rayleigh scattering spectra method mainly utilizes AussDNA and nanometer gold to mutually combine so as to form a stable AussDNA probe which can protect nanometer granules from being gathered by the higher concentration salt; when a solution exists As (III) in an HEPES (2-hydroxyethyl) buffer solution with pH of 8.2, As (III) and ssDNA in the AussDNA probe form a stable As-ssDNA compound, the released nanometer gold is gathered to form congeries with the larger particle diameter under the action of NaCl, and so that the syntony Rayleigh scattering peak intensity is strengthened; and more nanometer gold along with increase of concentration of the As(III) the AussDNA probe releases, the larger the aggregation of the nanometer gold is, and the syntony Rayleigh scattering peak intensity is strengthened. Therefore, the aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As(III) in water is established. A measuring method has the advantages that an instrument is simple, the operation is simple and rapid, the sensitivity is high, and the selectivity is good.
Description
Technical field
The present invention relates to analytical chemistry, specifically measure the aptamers of As in the water (III)-nm of gold Resonance Rayleigh Scattering Spectral Method.
Background technology
Arsenic is one of important environmental contaminants, spills in the surrounding environment with the form of raw ore or oxide, and atmosphere, water body, crops etc. are polluted.Arsenic can enter human body from respiratory tract, alimentary canal and skin, and the arsenic that enters in the body is discharged slowly, can accumulate for a long time, and human health has been caused very large threat.In environmental and biological samples, arsenic mainly exists with the form of As (III) and As (V), and arsenious toxicity is greater than pentavalent arsenic, and world health organisation recommendations 10 ng/mL are as the newest standards of potable water.Therefore, set up the method that a kind of high sensitivity, high selectivity detect arsenic ion, for environmental protection and human health all tool have very important significance.At present, the analytical approach of arsenic mainly contains colourimetry, electrochemical process, fluorescence method, chemoluminescence method, atomic absorption spectrography (AAS), inductively coupled plasma mass spectrometry etc.But the needs that these methods have are expensive and complicated instrument and equipment, and the method that has is complicated, and the sensitivity that has is not good enough, and the selectivity that has is not good enough, so that the use of these methods has been subject to certain restriction.
Aptamer be one section by tens or tens single strain oligonucleotides that nucleotide forms.Because aptamer has accurate identification, easily external synthetic and the characteristics such as modification, good stability, can specific binding target material, be used for analyzing and clinical examination.Resonance Rayleigh Scattering Spectral Method is a kind of easy, quick, sensitive spectral analysis new technology, has obtained in fields such as nucleic acid, protein, small molecule analysis to use preferably.It and aptamers reaction bonded have been set up the Resonance Rayleigh Scattering Spectral Method of some highly sensitive high selectivities.Nm of gold has good stability, good biocompatibility and Resonance Rayleigh Scattering effect, in aptamers analysis and the existing application of Resonance Rayleigh Scattering Spectra analysis.As far as we know, relevant aptamers modified nano gold Resonance Rayleigh Scattering Spectral Method detects arsenic ion and has no report.
Summary of the invention
The objective of the invention is for overcoming the deficiencies in the prior art, and the aptamers that a kind of Simple fast measures As (III) in the water-nm of gold Resonance Rayleigh Scattering Spectral Method is provided.
The technical scheme that realizes the object of the invention is:
Use the method for aptamers-nm of gold Resonance Rayleigh Scattering Spectral Method for Determination As (III), comprise the steps:
(1) preparation nm of gold aptamers probe (AussDNA): get the single stranded DNA that sequence is 5 '-TTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCG-3 ' (ssDNA), being dissolved to concentration with redistilled water is 390 nmol/L.Pipette 57.9 μ g/mL nm of gold, 6.0 mL in conical flask, under stirring condition, slowly add 1.8mL 390 nmol/L ssDNA solution, add rear continuation and stir 15min, seal 4 ℃ of preservations.Calculate with ssDNA, this AussDNA concentration is 90 nmol/L;
(2) preparation As (III) standard solution system: get 5 scale test tubes, pipette successively 50～300 μ L, 90 nmol/L AussDNA, the 4-hydroxyethyl piperazine ethanesulfonic acid sodium buffer solution (HEPES of 100～250 μ L, 50 mmol/L pH 8.2, contain 150 mmol/L NaCl), 5760 ng/mL As (III) standard solution that add respectively again 1～60 μ L, mixing, place ultrasonic 15 min on the ultrasonoscope, add again 20～80 μ L, 2.0 mol/L NaCl solution, be settled to 1.5 mL, mixing with redistilled water;
(3) prepare blank solution: do not add As (III) titer with the method for step (2) and prepare blank solution;
(4) get respectively by As (III) standard solution and the blank solution of step (2), (3) preparation in right amount, place cuvette, on fluorospectrophotometer, each solution of synchronous scanning obtains Resonance Rayleigh Scattering Spectra.278nm wavelength place measures the Resonance Rayleigh Scattering peak intensity of As (III) standard solution
I, and measure its blank solution Resonance Rayleigh Scattering peak intensity value (
I 0), calculate Δ
I=
I-
I 0
(5) with Δ
ITo the concentration relationship of As (III) curve of working;
(6) measured object sample determination: get the measured object water sample that contains As (III); Then pipette a certain amount of replacement As (III) standard solution, by step (2)～(4) operation.Calculate the Δ of measured object
I Sample=
I Sample-
I 0
(7) Δ that records per sample
I Sample, look into the working curve of step (5), calculate the concentration of As in the measured object water sample (III).
Realize that principle of the present invention is: ssDNA and nm of gold can by electric charge gravitation, Van der Waals force, and intermolecular force mutually combine and form more stable A
uThe ssDNA probe, the protection golden nanometer particle is not assembled by the salt of higher concentration.In pH 8.2 HEPES buffer solution, when having As (III) in the solution, ssDNA in As (III) and the AussDNA probe forms stable As-ssDNA compound, the nm of gold that discharges is assembled the larger aggregation of formation particle diameter under the NaCl effect, cause the Resonance Rayleigh Scattering peak intensity to strengthen.Along with As (III) concentration increases, the nm of gold that the AussDNA probe discharges is more, and the nm of gold aggregation is more, and the Resonance Rayleigh Scattering peak intensity strengthens.Can set up accordingly the aptamers nm of gold Resonance Rayleigh Scattering Spectral Method that detects As (III).
Advantage of the present invention is: compare with existing method, the instrument of this assay method is simple, and is easy and simple to handle, fast, highly sensitive, selectivity good.
Description of drawings
Fig. 1 is the partial resonance Rayleigh scattering light spectrogram that the embodiment of the invention is measured As (III).
Among the figure: a:12 nmol/L AussDNA – 200 μ L pH 8.2 HEPES-0.05 mol/L NaCl;
b:?a+76.8?ng/mL?As(III)；
c:?a+230.4?ng/mL?As(III)
Embodiment
Embodiment:
The aptamers of As (III)-nm of gold Resonance Rayleigh Scattering Spectral Method comprises the steps: in the mensuration water
(1) preparation nm of gold aptamers probe (AussDNA): get the single stranded DNA that sequence is 5 '-TTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCG-3 ' (ssDNA), being dissolved to concentration with redistilled water is 390 nmol/L.Pipette 57.9 μ g/mL nm of gold, 6.0 mL in conical flask, under stirring condition, slowly add 1.8mL 390 nmol/L ssDNA solution, add rear continuation and stir 15min, seal 4 ℃ of preservations.Calculate with ssDNA, this AussDNA concentration is 90 nmol/L;
(2) preparation As (III) standard solution system: get 5 scale test tubes, pipette successively 200 μ L, 90 nmol/L AussDNA, the 4-hydroxyethyl piperazine ethanesulfonic acid sodium buffer solution (HEPES of 200 μ L, 50 mmol/L pH 8.2, contain 150 mmol/L NaCl), 5760 ng/mL As (III) standard solution that add respectively again 1,10,20,30,60 μ L, mixing, place ultrasonic 15 min on the ultrasonoscope, add again 40 μ L, 2.0 mol/L NaCl solution, be settled to 1.5 mL, mixing with redistilled water;
(3) prepare blank solution: do not add As (III) standard solution with the method for step (2) and prepare blank solution;
(4) get respectively by As (III) standard solution and the blank solution of step (2), (3) preparation an amount of, place cuvette, on the F-7000 of Hitachi type fluorospectrophotometer, setting parameter PMT voltage is 450v, excite slit and emission slit to be 5nm, excitation wavelength equals that each solution of synchronous scanning obtains Resonance Rayleigh Scattering Spectra under the condition of emission wavelength.278nm wavelength place measures the Resonance Rayleigh Scattering peak intensity of As (III) standard solution
I, and measure its blank solution Resonance Rayleigh Scattering peak intensity value (
I 0), calculate Δ
I=
I-
I 0
(5) with Δ
IConcentration (unit is ng/mL) to As (III) concerns the curve of working, and its equation of linear regression is Δ
I=8.1
C+ 21;
(6) measured object sample determination: 3 in the measured object waste water sample that contains As (III) that the source of fetching is different; Then respectively pipette 1.0 mL and replace As (III) standard solution, 3 parts of replicate determinations are by step (2)～(4) operation.Calculate the Δ of measured object
I Sample=
I Sample-
I 0, the Δ of each water sample
I SampleMean value is respectively: 75,96,102;
(7) Δ that records per sample
I Sample, look into the working curve of step (5), calculate the concentration of As in the measured object water sample (III).
The embodiment of the invention is measured 3 of water samples, and As (III) content is respectively 6.7 ng/mL, 9.3 ng/mL, 10.0 ng/mL.
The range of linearity that the embodiment of the invention is measured As (III) is 3.8～230.4 ng/mL, and detection limit (3 α) is 1.9 ng/mL.
The checking of detection method of the present invention:
Get the water sample of known As (III), add As (III) standard solution of close concentration, operation steps is as follows:
(1) preparation nm of gold aptamers probe (AussDNA): get the single stranded DNA that sequence is 5 '-TTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCG-3 ' (ssDNA), being dissolved to concentration with redistilled water is 390 nmol/L.Pipette 57.9 μ g/mL nm of gold, 6.0 mL in conical flask, under stirring condition, slowly add 1.8mL 390 nmol/L ssDNA solution, add rear continuation and stir 15min, seal 4 ℃ of preservations.Calculate with ssDNA, this AussDNA concentration is 90 nmol/L;
(2) preparation As (III) standard solution system: get 5 scale test tubes, pipette successively 200 μ L, 90 nmol/L AussDNA, the 4-hydroxyethyl piperazine ethanesulfonic acid sodium buffer solution (HEPES of 200 μ L, 50 mmol/L pH 8.2, contain 150 mmol/L NaCl), 5760 ng/mL As (III) standard solution that add respectively again 1,10,20,30,60 μ L, mixing, place ultrasonic 15 min on the ultrasonoscope, add again 40 μ L, 2.0 mol/L NaCl solution, be settled to 1.5 mL, mixing with redistilled water;
(3) prepare blank solution: do not add As (III) standard solution with the method for step (2) and prepare blank solution;
(4) get respectively by As (III) standard solution and the blank solution of step (2), (3) preparation an amount of, place cuvette, on the F-7000 of Hitachi type fluorospectrophotometer, setting parameter PMT voltage is 450v, excite slit and emission slit to be 5nm, excitation wavelength equals that each solution of synchronous scanning obtains Resonance Rayleigh Scattering Spectra under the condition of emission wavelength.278nm wavelength place measures the Resonance Rayleigh Scattering peak intensity of As (III) standard solution
I, and measure its blank solution Resonance Rayleigh Scattering peak intensity value (
I 0), calculate Δ
I=
I-
I 0
(5) with Δ
IConcentration (unit is ng/mL) to As (III) concerns the curve of working, and its equation of linear regression is Δ
I=8.1
C+ 21;
(6) determination of recovery rates: in the scale test tube, accurately pipetting known As (III) concentration is each 0.5 mL of water sample of 6.7 ng/mL, 9.3 ng/mL, 10.0 ng/mL, three parts of replicate determinations, add respectively again 100 ng/mL As (III) standard solution, 50 μ L, mixing, by step (2), (4) operation, measure Δ
IMean value is respectively 66.0,72.9,74.5, the concentration of calculating measured As (III) is 5.56 ng/mL, 6.41 ng/mL, 6.60 ng/mL, the recovery is respectively 99.8%, 99.7%, 99.0%, relative standard deviation is respectively 2.9%, 3.2%, 2.6%, meet the requirement of analytical error, illustrate that the method accurately and reliably.
Claims (1)
1. the aptamers of measuring As (III) in the water-nm of gold Resonance Rayleigh Scattering Spectral Method is characterized in that: comprise the steps:
(1) preparation nm of gold aptamers probe (AussDNA): get the single stranded DNA that sequence is 5 '-TTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCG-3 ' (ssDNA), being dissolved to concentration with redistilled water is 390 nmol/L; Pipette 57.9 μ g/mL nm of gold, 6.0 mL in conical flask, under stirring condition, slowly add 1.8mL 390 nmol/L ssDNA solution, add rear continuation and stir 15min, seal 4 ℃ of preservations; Calculate with ssDNA, this AussDNA concentration is 90 nmol/L;
(2) preparation As (III) standard solution: get 5 scale test tubes, pipette successively 200 μ L, 90 nmol/L AussDNA, the 4-hydroxyethyl piperazine ethanesulfonic acid sodium buffer solution (HEPES of 200 μ L, 50 mmol/L pH 8.2, contain 150 mmol/L NaCl), 5760 ng/mL As (III) standard solution that add respectively again 1,10,20,30,60 μ L, mixing, place ultrasonic 15 min on the ultrasonoscope, add again 40 μ L, 2.0 mol/L NaCl solution, be settled to 1.5 mL, mixing with redistilled water;
(3) prepare blank solution: do not add As (III) standard solution with the method for step (2) and prepare blank solution;
(4) get respectively by As (III) standard solution and the blank solution of step (2), (3) preparation an amount of, place cuvette, on fluorospectrophotometer, set that each solution of synchronous scanning obtains Resonance Rayleigh Scattering Spectra under instrument parameter equals emission wavelength in excitation wavelength the condition; 278nm wavelength place measures the Resonance Rayleigh Scattering peak intensity of As (III) standard solution
I, and measure its blank solution Resonance Rayleigh Scattering peak intensity value (
I 0), calculate Δ
I=
I-
I 0
(5) with Δ
ITo the concentration relationship of As (III) curve of working;
(6) measured object sample determination: get the measured object water sample that contains As (III); Then pipette a certain amount of replacement As (III) standard solution, by step (2)～(4) operation, calculate the Δ of measured object
I Sample=
I Sample-
I 0
(7) Δ that records per sample
I Sample, look into the working curve of step (5), calculate the concentration of As in the measured object water sample (III).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103643066A CN102998288A (en) | 2012-09-26 | 2012-09-26 | Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103643066A CN102998288A (en) | 2012-09-26 | 2012-09-26 | Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102998288A true CN102998288A (en) | 2013-03-27 |
Family
ID=47927131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103643066A Pending CN102998288A (en) | 2012-09-26 | 2012-09-26 | Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102998288A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103364353A (en) * | 2013-07-19 | 2013-10-23 | 广西师范大学 | Aptamer nanogold resonance Rayleigh scattering spectrum method for determination of lysozyme |
| CN106885805A (en) * | 2017-03-31 | 2017-06-23 | 安徽师范大学 | Chemical biosensor, preparation method and application that coagulation based on gold nano under high concentration salt solutions builds |
| CN107044963A (en) * | 2017-03-31 | 2017-08-15 | 上海理工大学 | A kind of new arsenic aptamers nucleotide sequence and the application for detecting arsenic ion |
| CN110646419A (en) * | 2019-10-28 | 2020-01-03 | 上海交通大学 | Method for detecting trivalent arsenic in water body by using short single-stranded DNA |
| CN110987591A (en) * | 2019-12-16 | 2020-04-10 | 苏州市纤维检验院 | Method for simultaneously determining arsenic and mercury in textile through microwave digestion |
| CN111007038A (en) * | 2019-11-29 | 2020-04-14 | 太原理工大学 | Device and method for quantitatively detecting arsenic ions in water based on laser photo-thermal interference |
| CN111344551A (en) * | 2017-10-23 | 2020-06-26 | 美国政府(由卫生和人类服务部的部长所代表) | Optical configuration method of spectrum scattering flow cytometer |
| US11536719B2 (en) | 2016-10-21 | 2022-12-27 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Molecular nanotags |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003066025A (en) * | 2001-08-28 | 2003-03-05 | Tohoku Techno Arch Co Ltd | Simple detection method of trace quantity of arsenic in water |
| CN1475794A (en) * | 2003-07-16 | 2004-02-18 | 济南市疾病预防控制中心 | Measuring method of arsenic in food, health care product and biological sample |
| JP2005172755A (en) * | 2003-12-15 | 2005-06-30 | Tohoku Techno Arch Co Ltd | Method for measuring arsenic |
| CN1963471A (en) * | 2006-11-30 | 2007-05-16 | 云南出入境检验检疫局检验检疫技术中心 | Testing method for harmful element arsenic of cigarette paper |
| CN101140226A (en) * | 2007-09-04 | 2008-03-12 | 山东中烟工业公司 | Method for detecting arsenic in scenting agent with AOTF near-infrared spectrometer |
| US20090200486A1 (en) * | 2008-02-13 | 2009-08-13 | Nianqiang Wu | Quantum dot-DNA-metallic nanoparticle ensemble as fluorescent nanosensor system for multiplexed detection of heavy metals |
| CN101620186A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院上海硅酸盐研究所 | Method for analyzing arsenic content in glass refining agent |
| CN101650302A (en) * | 2009-09-14 | 2010-02-17 | 中国一拖集团有限公司 | Test method of micro amount of arsenic or antimony in steel |
| CN101726473A (en) * | 2009-10-29 | 2010-06-09 | 广西师范大学 | Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold |
| CN101776608A (en) * | 2009-10-29 | 2010-07-14 | 广西师范大学 | Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum |
| CN102288568A (en) * | 2011-07-29 | 2011-12-21 | 广西师范大学 | Method for rapidly measuring nanogold catalysis-silver nitrate reduction luminosity of UO2<2+> in water |
| CN102435587A (en) * | 2011-12-06 | 2012-05-02 | 广西师范大学 | Method for rapidly determining nitrite in water through nanogold resonance scattering spectrometry |
| CN102661925A (en) * | 2012-05-15 | 2012-09-12 | 力合科技（湖南）股份有限公司 | Method for detecting arsenic content in water body |
-
2012
- 2012-09-26 CN CN2012103643066A patent/CN102998288A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003066025A (en) * | 2001-08-28 | 2003-03-05 | Tohoku Techno Arch Co Ltd | Simple detection method of trace quantity of arsenic in water |
| CN1475794A (en) * | 2003-07-16 | 2004-02-18 | 济南市疾病预防控制中心 | Measuring method of arsenic in food, health care product and biological sample |
| JP2005172755A (en) * | 2003-12-15 | 2005-06-30 | Tohoku Techno Arch Co Ltd | Method for measuring arsenic |
| CN1963471A (en) * | 2006-11-30 | 2007-05-16 | 云南出入境检验检疫局检验检疫技术中心 | Testing method for harmful element arsenic of cigarette paper |
| CN101140226A (en) * | 2007-09-04 | 2008-03-12 | 山东中烟工业公司 | Method for detecting arsenic in scenting agent with AOTF near-infrared spectrometer |
| US20090200486A1 (en) * | 2008-02-13 | 2009-08-13 | Nianqiang Wu | Quantum dot-DNA-metallic nanoparticle ensemble as fluorescent nanosensor system for multiplexed detection of heavy metals |
| CN101620186A (en) * | 2009-07-27 | 2010-01-06 | 中国科学院上海硅酸盐研究所 | Method for analyzing arsenic content in glass refining agent |
| CN101650302A (en) * | 2009-09-14 | 2010-02-17 | 中国一拖集团有限公司 | Test method of micro amount of arsenic or antimony in steel |
| CN101726473A (en) * | 2009-10-29 | 2010-06-09 | 广西师范大学 | Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold |
| CN101776608A (en) * | 2009-10-29 | 2010-07-14 | 广西师范大学 | Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum |
| CN102288568A (en) * | 2011-07-29 | 2011-12-21 | 广西师范大学 | Method for rapidly measuring nanogold catalysis-silver nitrate reduction luminosity of UO2<2+> in water |
| CN102435587A (en) * | 2011-12-06 | 2012-05-02 | 广西师范大学 | Method for rapidly determining nitrite in water through nanogold resonance scattering spectrometry |
| CN102661925A (en) * | 2012-05-15 | 2012-09-12 | 力合科技（湖南）股份有限公司 | Method for detecting arsenic content in water body |
Non-Patent Citations (7)
| Title |
|---|
| JIANG ZHILIANG ET AL.: "Resonance Scattering Spectral Detection of Trace Hg2+ Using Aptamer-Modified Nanogold as Probe and Nanocatalyst", 《ANALYTICAL CHEMISTRY》 * |
| KIM MINA ET AL.: "Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
| YUANGEN WU ET AL.: "Cationic polymers and aptamers mediated aggregation of gold nanoparticles for the colorimetric detection of arsenic(III) in aqueous solution", 《CHEMICAL COMMUNICATION》 * |
| YUANGEN WU ET AL.: "Ultrasensitive aptamer biosensor for arsenic(Ⅲ) detection in aqueous solution based on surfactant-induced aggregation of gold nanoparticles", 《ANALYST》 * |
| 刘云富 等: "共振瑞利散射法测定环境水样中痕量砷（Ⅲ）", 《光谱实验室》 * |
| 曹静祥 等: "不同饮水砷测定方法的比对", 《中国地方病学杂志》 * |
| 蒋治良 等: "金纳米粒子的共振散射光谱", 《中国科学B辑》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103364353A (en) * | 2013-07-19 | 2013-10-23 | 广西师范大学 | Aptamer nanogold resonance Rayleigh scattering spectrum method for determination of lysozyme |
| CN103364353B (en) * | 2013-07-19 | 2015-08-05 | 广西师范大学 | A kind of aptamer nanogold Resonance Rayleigh Scattering Spectra method measuring lysozyme |
| US11536719B2 (en) | 2016-10-21 | 2022-12-27 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Molecular nanotags |
| CN106885805A (en) * | 2017-03-31 | 2017-06-23 | 安徽师范大学 | Chemical biosensor, preparation method and application that coagulation based on gold nano under high concentration salt solutions builds |
| CN107044963A (en) * | 2017-03-31 | 2017-08-15 | 上海理工大学 | A kind of new arsenic aptamers nucleotide sequence and the application for detecting arsenic ion |
| CN106885805B (en) * | 2017-03-31 | 2019-06-25 | 安徽师范大学 | Based on the chemical biosensor of the coagulation building of gold nano, preparation method and application under high concentration salt solutions |
| CN111344551B (en) * | 2017-10-23 | 2021-07-20 | 美国政府(由卫生和人类服务部的部长所代表) | Optical configuration method of spectrum scattering flow cytometer |
| CN111344551A (en) * | 2017-10-23 | 2020-06-26 | 美国政府(由卫生和人类服务部的部长所代表) | Optical configuration method of spectrum scattering flow cytometer |
| CN110646419A (en) * | 2019-10-28 | 2020-01-03 | 上海交通大学 | Method for detecting trivalent arsenic in water body by using short single-stranded DNA |
| CN111007038A (en) * | 2019-11-29 | 2020-04-14 | 太原理工大学 | Device and method for quantitatively detecting arsenic ions in water based on laser photo-thermal interference |
| CN111007038B (en) * | 2019-11-29 | 2022-07-05 | 太原理工大学 | Device and method for quantitatively detecting arsenic ions in water based on laser photo-thermal interference |
| CN110987591A (en) * | 2019-12-16 | 2020-04-10 | 苏州市纤维检验院 | Method for simultaneously determining arsenic and mercury in textile through microwave digestion |
| CN110987591B (en) * | 2019-12-16 | 2022-04-05 | 苏州市纤维检验院 | Method for simultaneously determining arsenic and mercury in textile through microwave digestion |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102998288A (en) | Aptamer-nanometer gold syntony Rayleigh scattering spectra method for measuring As (III) in water | |
| CN103364353B (en) | A kind of aptamer nanogold Resonance Rayleigh Scattering Spectra method measuring lysozyme | |
| US9983191B2 (en) | Nanopore detection of small molecules through competition assays | |
| US10837954B2 (en) | Nanopore detection of small molecules through competition assays | |
| Hu et al. | Double-strand DNA-templated synthesis of copper nanoclusters as novel fluorescence probe for label-free detection of biothiols | |
| CN102925136B (en) | Zn<2+> ratiometric fluorescent probe compound and preparation method and use thereof | |
| CN109142710B (en) | Method for rapidly and sensitively detecting tetrodotoxin TTX | |
| CN105510420A (en) | Method for determining ATP content on basis of magnetic bead separation and DNA marker gold nanoparticle probe | |
| CN104614355A (en) | Method for detecting concentration of Cu<2+> based on bovine serum albumin functionalized gold nanocluster light scattering probe | |
| CN105699349A (en) | Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof | |
| CN104151480B (en) | A kind of there is pH value and mercury ion ratio test function water dispersible fluorescent polymer nanoparticle, preparation method and application | |
| CN103604792A (en) | Resonance Rayleigh scattering (RRS) method for measuring bromide ion | |
| CN109777412A (en) | A kind of pair of transmitting fluorescent carbon point and its preparation method and application | |
| CN105131935A (en) | Rapid high-selectivity fluorine ion colorimetric probe and preparation method therefor | |
| Saleh | Detection: from electrochemistry to spectroscopy with chromatographic techniques, recent trends with nanotechnology | |
| CN105651749A (en) | Method for detecting water content in tetrahydrofuran through carbon nanoparticles | |
| CN105588823A (en) | Preparation and application of fluorescent switch type sensor for detecting biological thiols | |
| CN102435587B (en) | Method for rapidly determining nitrite in water through nanogold resonance scattering spectrometry | |
| CN103278487A (en) | Method for measuring bisphenol A in plastic product by means of aptamer-nanogold resonance rayleigh scattering spectrometry | |
| CN101706500A (en) | Method for analyzing quantum dot-enhanced high-sensitivity DNA adduct | |
| CN103575715B (en) | A kind of method detecting mitoxantrone based on luminescent gold nano-cluster | |
| CN102661943B (en) | Method for measuring cystine through surface-enhanced raman spectroscopy | |
| CN109187469A (en) | A method of with enzymatic oxidation TMB fluorescence spectrometry glucose | |
| CN108827921B (en) | Room-temperature phosphorescence detection method for lysozyme and application | |
| CN104237185B (en) | PH value measurement method based on N-acetyl-L-cysteine-gold nanocluster |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130327 |