CN102994369A - Chip structure for PCR (polymerase chain reaction) rapid reaction - Google Patents
Chip structure for PCR (polymerase chain reaction) rapid reaction Download PDFInfo
- Publication number
- CN102994369A CN102994369A CN2012105421953A CN201210542195A CN102994369A CN 102994369 A CN102994369 A CN 102994369A CN 2012105421953 A CN2012105421953 A CN 2012105421953A CN 201210542195 A CN201210542195 A CN 201210542195A CN 102994369 A CN102994369 A CN 102994369A
- Authority
- CN
- China
- Prior art keywords
- pcr
- chip
- cover plate
- quick response
- chip structure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a chip structure for PCR (polymerase chain reaction) rapid reaction. The chip structure for PCR rapid reaction comprises a cover plate, a medium layer, a PCR chip and an external heating structure; through holes are machined on the cover plate; micro cavity holes are arranged on the PCR chip; the medium layer is covered on the PCR chip, and absorbs the cover plate in a negative pressure mode through the through holes on the cover plate; the lower bottom surface of the PCR chip are in tight contact with the external heating structure; the PCR chip can be a single-layer structure, and the micro cavity holes are directly machined; or the PCR chip can be a multi-layer structure, and micro cavity holes are machined on at least one layer, and are then combined with the other base material. PCR amplification is easy to achieve rapidly, and the biochemical reaction speed is improved obviously; the heat capacity of a micro environment of a chip is small, and temperature rising and reduction can be controlled at high speed, and the regional temperature can be detected and controlled accurately; the temperature cycle system volume is reduced, the heat capacity is reduced, and the reaction time is shortened greatly; the reaction liquid volume is reduced, the amplification specificity is intensified, and not only is cost saved, but also the efficiency is improved.
Description
Technical field
The present invention relates to a kind ofly for PCR quick response chip structure, belong to the round pcr field.
Background technology
Polymerase chain reaction (PCR) is a kind of Protocols in Molecular Biology, is used for amplifying specific dna fragmentation, can regard the special dna replication dna of in vitro as.The round pcr principle is similar to the natural reproduction process of DNA, and its specificity depends on the Oligonucleolide primers with the complementation of target sequence two ends.PCR is made of sex change-annealing-extension three basic reactions steps: the 1. sex change of template DNA: template DNA is after being heated to 94 ℃ of left and right sides certain hours, template DNA double-stranded DNA double-stranded or that form through pcr amplification is dissociated, make it to become strand, in order to be combined with primer, for the lower whorl reaction is prepared; 2. the annealing (renaturation) of template DNA and primer: template DNA is after heat denatured becomes strand, and temperature is down to about 50-65 ℃, the complementary sequence pairing combination of primer and template DNA strand; 3. the extension of primer: dna profiling-primer binding substances is under the effect of Taq archaeal dna polymerase, take dNTP as reaction raw materials, target sequence is template, press base complementrity pairing and semiconservative replication principle, synthetic new and the semiconservative replication chain complementation of template DNA chain, recirculation sex change-annealing-extension three processes just can obtain more " semiconservative replication chain ", and this new chain can become again the template of circulation next time.Round pcr has been important development direction in life science and the medical field development, becomes a kind of detection means and technological method of widespread use, has wide using value at aspects such as life science, engineering in medicine, genetics, forensic identifications.
The key of pcr amplification is to control fast and accurately temperature cycle, there is the shortest effective reaction time in each step in the pcr amplification circulation, temperature changing process is oversize not only loses time, and descend gradually along with the activity of the prolongation Taq archaeal dna polymerase of time, therefore long reaction process is very disadvantageous to PCR.The thinking that therefore the PCR reaction efficiency directly is provided is exactly to reduce the volume of PCR mixed solution, can shorten cycling time, improves the content of amplified production.Static cavate pcr chip chip has following advantage:
1) the temperature circulatory system volume reduces, and thermal capacitance reduces, can reach very high lifting/lowering temperature speed (even can be up to 60-90 ℃/s), the reaction times shortens at double;
2) the reaction solution volume reduces, and the consumption of reaction reagent reduces, and the homogeneity of reacting liquid temperature improves, and the specificity of amplification strengthens, and both provides cost savings, and has improved again efficient;
3) can select the high material of thermal conductivity, promote heat transfer rate, greatly reduce time and required the expending time in of circulation of reacting liquid temperature balance, temperature can be stablized and detect faster.
Static micro chamber type pcr chip is relatively simple for structure, and the normal operation micro-processing technology processes the micro chamber (micro chamber has the fluid inlet and outlet that can seal) of reaction, is used for electrode and cooling and the heat abstractor of heating and temperature sensing.Its ultimate principle is close with the normal PCR instrument, is the PCR reaction mixture that injects in the reaction chamber is carried out direct heating and cooling control, realizes temperature cycle.Although simple in structure, because the micro-scale effect, thermal inertia is little, the PCR reaction solution can carry out heating and cooling work soon in micro chamber, and thermal sensor also can in time feed back and temperature be controlled.Because the micro chamber chip structure is simple, also means and at a plurality of reaction tanks of chip piece processing, to carry out simultaneously a plurality of PCR reactions.Micro chamber is made by the method for micromachined and is obtained.These characteristics all have very large advantage in actual use.Static micro chamber type pcr chip has reduced the consumption of mixed solution significantly, but temperature control system and structure design directly determine heating and cooling speed, still to temperature cycle and the stable decisive role that plays, in general with desirable heating and cooling speed of response old certain gap still.General heating needs integrated processing on chip with heat abstractor, this is so that also to some extent rising of tooling cost, and the reagent of traditional static chamber pcr chip is prepared, the rear reagent of reaction extracts still need to have outside transfer process, may cause the waste of reagent and the appearance of transferring the pollution.Therefore, the many warm areas circulations of the independence of realization PCR become one of direction of static chamber pcr chip development.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists, provide a kind of for PCR quick response chip structure.
Purpose of the present invention is achieved through the following technical solutions:
Be used for PCR quick response chip structure, characteristics are: comprise the cover plate, medium layer, pcr chip and the indirect heating structure that set gradually from top to down, be processed with through hole on the described cover plate, described pcr chip is provided with microcavity aperture, cover medium layer on the pcr chip, medium layer presents the negative-pressure adsorption cover plate by the through hole on the cover plate, and the bottom surface of pcr chip contacts with the indirect heating close structure.
Further, the above-mentioned PCR quick response chip structure that is used for, wherein, the thickness of described pcr chip is 100 μ m～10mm, the volume of microcavity aperture is 0.01 μ L ~ 10mL on the pcr chip.
Further, the above-mentioned PCR quick response chip structure that is used for, wherein, described pcr chip is any one material or any bi-material compound at least in silica glass, aluminium sesquioxide, quartz, glass, silicon, polycarbonate, tetrafluoroethylene, polyethylene terephthalate, polysulfones, the polyvinylidene difluoride (PVDF); Described arbitrarily at least bi-material join compound by bonding, bonding or ultrasonic welding mode.
Further, the above-mentioned PCR quick response chip structure that is used for, wherein, described cover plate is any one material or any bi-material compound at least in polycarbonate, polymethyl methacrylate, glass, silica glass, polydimethylsiloxane, the silica gel material.
Again further, the above-mentioned PCR quick response chip structure that is used for, wherein, described medium layer is any one material or any bi-material compound at least in Resins, epoxy, polymethyl methacrylate, polydimethylsiloxane, polycarbonate, cyclic olefine copolymer, polyphenylene ethyl, polyimide, silicon rubber, polypropylene, the polyethylene terephthalate; The thickness of described medium layer is 10nm ~ 10mm.
Again further, the above-mentioned PCR quick response chip structure that is used for, wherein, described indirect heating structure comprises film heating sheet and the thermally conductive material bonding with it; Described thermally conductive material is any one material or any bi-material compound at least in metal, metal oxide, silicon, silicon carbide, graphite, thermal conductive silicon ketone resin, the heat-conducting plastic.
Again further, the above-mentioned PCR quick response chip structure that is used for, wherein, being shaped as of described microcavity aperture is cylindric or round table-like.
The substantive distinguishing features that technical solution of the present invention is outstanding and significant progressive being mainly reflected in:
The present invention is easy to realize fast pcr amplification, significantly improves the speed of biochemical reaction; The thermal capacity of the microenvironment of chip is little, can carry out heating and cooling control at a high speed, and the temperature in the accurate Detection ﹠ Controling zone of energy; The temperature circulatory system volume reduces, and thermal capacitance reduces, and can reach very high lifting/lowering temperature speed, and the reaction times shortens greatly; The reaction solution volume reduces, and the homogeneity of reacting liquid temperature improves, and the specificity of amplification strengthens, and has not only provided cost savings but also has improved efficient; Chip is easy to integrated and functionalization, realizes quickly and easily amplification, is convenient to the mass low cost production.
Description of drawings
Below in conjunction with accompanying drawing technical solution of the present invention is described further:
Fig. 1: cross section structure synoptic diagram of the present invention;
Fig. 2: the schematic top plan view of cover plate;
Fig. 3: the schematic top plan view of pcr chip.
Embodiment
As shown in Figure 1, be used for PCR quick response chip structure, comprise the cover plate 1, medium layer 2, pcr chip 3 and the indirect heating structure 4 that set gradually from top to down, such as Fig. 2, be processed with through hole 101 on the cover plate 1, such as Fig. 3, pcr chip 3 is provided with microcavity aperture 301, and the degree of depth of microcavity aperture 301 is covered medium layer less than the thickness of chip on the pcr chip, medium layer presents the negative-pressure adsorption cover plate by the through hole on the cover plate, and the bottom surface of pcr chip contacts with the indirect heating close structure.
Cover plate 1 joins by medium layer 2 and pcr chip 3, and the thickness of pcr chip 3 is 100 μ m～10mm, and the volume of microcavity aperture 301 is 0.01 μ L ~ 10mL on the pcr chip, and it is cylindric or round table-like that the shape of microcavity aperture is.
Cover plate 1 is any one material or any bi-material compound at least in polycarbonate (PC), polymethyl methacrylate (PMMA), glass, silica glass, polydimethylsiloxane (PDMS), the silica gel material.
Indirect heating structure 4 comprises film heating sheet and the thermally conductive material bonding with it; Thermally conductive material is any one material or any bi-material compound at least in metal, metal oxide, silicon, silicon carbide, graphite, thermal conductive silicon ketone resin, the heat-conducting plastic.
Put into PCR solution in the microcavity aperture 301 on the pcr chip 3, then by cover plate 1 sealing, utilize outside heating arrangement to carry out pcr amplification.Be provided with one deck medium layer 2 between cover plate 1 and the pcr chip 3, each replacing deielectric-coating that uses gets final product.Medium layer 2 presents the negative-pressure adsorption cover plate by the through hole of cover plate top, and pcr amplification returns to malleation when finishing, to be convenient for changing deielectric-coating.Indirect heating structure 4 is by heat conduction good material and directly contacting fully that the lower surface of pcr chip 3 is tried one's best, to reach the effect of rapid temperature rise and drop.Heating temperature continuous transformation between three temperature, temperature-fall period are the nature coolings, to realize pcr amplification.The detailed process of a pcr amplification circulation is: put into PCR solution in the microcavity aperture 301 on pcr chip 3, cover plate 1 absorption one deck medium layer 2, then apply certain pressure and microcavity aperture face seal (maintenance adsorbed state) at cover plate 1, then indirect heating structure 4 heats, and at three temperature among self-sustaining certain hours, lowering the temperature is naturally cooling, realize pcr amplification, remove cover plate 1, the deielectric-coating of absorption is removed, and takes out PCR solution and carries out subsequent experimental.
Embodiment 1
Pcr chip: adopting quartz glass is as base material, be of a size of 80mm * 20mm, thickness is 2mm, use laser boring technique, in quartz glass plate processing two row's microcavity aperture, every row is the microcavity aperture of eight same apertures, in two row's microcavity aperture, the diameter of one row's microcavity aperture is 1.6mm, the diameter of another row's microcavity aperture is 3.0mm, and the degree of depth is 1.5mm, and the spacing between the microcavity aperture center is 9mm, distance between microcavity aperture center and the long limit is 8.5mm, and the distance between microcavity aperture center and the minor face is 5.5mm.Silica glass chip after the processing need to pass through ultrasonic cleaning, and technique is: in acetone soln, clean 5min; Take out and directly put into ethanolic soln, clean 5min, directly put into deionized water after the taking-up, clean 5min, nitrogen dries up.
Cover plate: adopt PC sheet material matter as mechanically resistant material, be processed into 80mm * 20mm size, thickness is 1.5mm, the silica gel piece that 0.5mm is thick also is processed into 80mm * 20mm size, then use structure glue that PC plate and silica gel piece are bonded together, and on top process the through hole that diameter is 2mm, utilize through hole adsorption medium layer.
Medium layer: adopt the PET film as medium layer, the length and width size is respectively 80mm and 20mm.
The indirect heating structure: adopt the film heating sheet as thermal source, silicon chip is as heat-conduction medium.The film heating sheet is made into the length and width size is respectively 80mm and 20mm, thickness is that the silicon chip of 500 μ m also is processed into the length and width size and is respectively 80mm and 20mm, then uses heat-conducting glue that film heating sheet and silicon chip are bonded together and consists of the indirect heating structure.
When being used for pcr amplification, its processing step is:
1) bottom surface and the indirect heating close structure with pcr chip contacts;
2) on pcr chip, diameter is the PCR solution that injects respectively 3 μ L in eight microcavity aperture of 1.6mm, and diameter is the formamide soln that injects respectively 10 μ L in eight microcavity aperture of 3.0mm;
3) the mechanically resistant material upper surface with cover plate links to each other with outside vacuum extractor, makes it have suction, absorption one deck PET film, the sealing of then will joining with the upper surface of the cover plate of film and pcr chip;
4) the indirect heating structure heats, and three temperature change value are respectively 95 ℃-59 ℃-72 ℃ with order, and temperature-fall period is the nature cooling, and iterative cycles is finished pcr amplification 30 times.
Pcr chip: adopt 7740 glass and silicon chip as chip substrates, 7740 glass diameters are of a size of 100mm, and thickness is 1.5mm, mark the unit of two 80mm * 20mm size at sheet glass.Use laser boring technique, it is through hole that there are two row's microcavity aperture each unit, every row is contained the microcavity aperture of eight same apertures, in the same unit two row's microcavity aperture diameter is 1.6mm and 3.0mm respectively, spacing between the microcavity aperture center is 9mm, distance between the long limit of microcavity aperture center and unit is 8.5mm, and the distance between through hole center and the unit minor face is 5.5mm.The silicon chip diameter dimension is 100mm, and thickness is 500 μ m.Utilize the anode linkage technology, the sheet glass that contains through hole and silicon chip after cleaning are bonded together, form pcr chip.Sheet glass after the processing and silicon chip all need through ultrasonic cleaning, and technique is: in acetone soln, clean 5min; Take out and directly put into ethanolic soln, clean 5min, directly put into deionized water after the taking-up, clean 5min, nitrogen dries up.
The working method of cover plate, medium layer and indirect heating structure is identical with embodiment 1.
Can find out that pcr chip can be one deck structure, directly on top process microcavity aperture; Or multilayered structure, process microcavity aperture at one deck at least, compound by bonding, mode and another base material bonding or welding again, during multilayered structure, the material of layer can difference; So that realization fast PCR.
The present invention compares with existing pcr chip structure, highlights following characteristics
(1) static cavate pcr chip can improve the speed of biochemical reaction, is convenient to mass and produces cheaply;
(2) thermal capacity of the microenvironment of chip is little, can carry out heating and cooling control at a high speed, and the temperature in the accurate Detection ﹠ Controling zone of energy;
(3) the temperature circulatory system volume reduces, and thermal capacitance reduces, can reach very high lifting/lowering temperature speed (even can be up to 60-90 ℃/s), the reaction times significantly shortens;
(4) the reaction solution volume reduces, and the homogeneity of reacting liquid temperature improves, and the specificity of amplification strengthens, and has not only provided cost savings but also has improved efficient;
(5) chip is easy to integrated and functionalization, can realize quickly and easily amplification.
What need to understand is: the above only is preferred implementation of the present invention; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. be used for PCR quick response chip structure, it is characterized in that: comprise the cover plate, medium layer, pcr chip and the indirect heating structure that set gradually from top to down, be processed with through hole on the described cover plate, described pcr chip is provided with microcavity aperture, cover medium layer on the pcr chip, medium layer presents the negative-pressure adsorption cover plate by the through hole on the cover plate, and the bottom surface of pcr chip contacts with the indirect heating close structure.
2. according to claim 1 for PCR quick response chip structure, it is characterized in that: the thickness of described pcr chip is 100 μ m～10mm, and the volume of microcavity aperture is 0.01 μ L ~ 10mL on the pcr chip.
3. according to claim 1 and 2 for PCR quick response chip structure, it is characterized in that: described pcr chip is any one material or any bi-material compound at least in silica glass, aluminium sesquioxide, quartz, glass, silicon, polycarbonate, tetrafluoroethylene, polyethylene terephthalate, polysulfones, the polyvinylidene difluoride (PVDF).
4. according to claim 3 for PCR quick response chip structure, it is characterized in that: described arbitrarily at least bi-material join compound by bonding, bonding or ultrasonic welding mode.
5. according to claim 1 for PCR quick response chip structure, it is characterized in that: described cover plate is any one material or any bi-material compound at least in polycarbonate, polymethyl methacrylate, glass, silica glass, polydimethylsiloxane, the silica gel material.
6. according to claim 1 for PCR quick response chip structure, it is characterized in that: described medium layer is any one material or any bi-material compound at least in Resins, epoxy, polymethyl methacrylate, polydimethylsiloxane, polycarbonate, cyclic olefine copolymer, polyphenylene ethyl, polyimide, silicon rubber, polypropylene, the polyethylene terephthalate.
7. according to claim 1 or 6 described for PCR quick response chip structure, it is characterized in that: the thickness of described medium layer is 10nm ~ 10mm.
8. according to claim 1 for PCR quick response chip structure, it is characterized in that: described indirect heating structure comprises film heating sheet and the thermally conductive material bonding with it.
9. according to claim 8 for PCR quick response chip structure, it is characterized in that: described thermally conductive material is any one material or any bi-material compound at least in metal, metal oxide, silicon, silicon carbide, graphite, thermal conductive silicon ketone resin, the heat-conducting plastic.
10. according to claim 1 for PCR quick response chip structure, it is characterized in that: being shaped as of described microcavity aperture is cylindric or round table-like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210542195.3A CN102994369B (en) | 2012-12-14 | 2012-12-14 | Chip structure for PCR (polymerase chain reaction) rapid reaction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210542195.3A CN102994369B (en) | 2012-12-14 | 2012-12-14 | Chip structure for PCR (polymerase chain reaction) rapid reaction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102994369A true CN102994369A (en) | 2013-03-27 |
| CN102994369B CN102994369B (en) | 2014-12-31 |
Family
ID=47923485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210542195.3A Active CN102994369B (en) | 2012-12-14 | 2012-12-14 | Chip structure for PCR (polymerase chain reaction) rapid reaction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102994369B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451088A (en) * | 2013-08-23 | 2013-12-18 | 上海交通大学 | Micro-droplet type PCR (polymerase chain reaction) chip and manufacture method thereof |
| CN103589630A (en) * | 2013-11-18 | 2014-02-19 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
| CN104593256A (en) * | 2015-01-06 | 2015-05-06 | 上海交通大学 | PCR chip with repeatedly used electrode |
| CN105296351A (en) * | 2015-12-02 | 2016-02-03 | 北京大学 | Chip for polymerase chain reaction (PCR), and real-time detection device and system |
| CN108117983A (en) * | 2016-11-30 | 2018-06-05 | 希森美康株式会社 | Subject processing unit, subject processing method and subject processing chip |
| EP3420107A4 (en) * | 2016-02-22 | 2019-07-31 | BioFire Defense, LLC | Devices and methods for rapid pcr |
| CN110191759A (en) * | 2016-12-19 | 2019-08-30 | 碧佛尔酷瑞公司 | Micro-fluidic sample chip uses the analysis system of the chip and the PCR method for detecting DNA sequence dna |
| CN110452814A (en) * | 2019-07-12 | 2019-11-15 | 北京资和源医疗科技有限公司 | A kind of PCR fast reaction chip |
| WO2022246723A1 (en) * | 2021-05-27 | 2022-12-01 | 京东方科技集团股份有限公司 | Detection chip, and preparation method therefor and sample feeding method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020083686A1 (en) * | 2000-09-29 | 2002-07-04 | Audino Deborah C. | Heat sealing septum for storage plates |
| US20080293127A1 (en) * | 2007-05-22 | 2008-11-27 | Applera Corporation | Method to Render Pressure Sensitive Adhesive Compatible with Polymerase Chain Reaction Systems |
| CN203096061U (en) * | 2012-12-14 | 2013-07-31 | 凯晶生物科技(苏州)有限公司 | Chip structure for PCR (polymerase chain reaction) fast reaction |
-
2012
- 2012-12-14 CN CN201210542195.3A patent/CN102994369B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020083686A1 (en) * | 2000-09-29 | 2002-07-04 | Audino Deborah C. | Heat sealing septum for storage plates |
| US20080293127A1 (en) * | 2007-05-22 | 2008-11-27 | Applera Corporation | Method to Render Pressure Sensitive Adhesive Compatible with Polymerase Chain Reaction Systems |
| CN203096061U (en) * | 2012-12-14 | 2013-07-31 | 凯晶生物科技(苏州)有限公司 | Chip structure for PCR (polymerase chain reaction) fast reaction |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451088B (en) * | 2013-08-23 | 2015-01-21 | 上海交通大学 | Micro-droplet type PCR (polymerase chain reaction) chip and manufacture method thereof |
| CN103451088A (en) * | 2013-08-23 | 2013-12-18 | 上海交通大学 | Micro-droplet type PCR (polymerase chain reaction) chip and manufacture method thereof |
| CN103589630A (en) * | 2013-11-18 | 2014-02-19 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
| CN103589630B (en) * | 2013-11-18 | 2015-07-22 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
| CN104593256A (en) * | 2015-01-06 | 2015-05-06 | 上海交通大学 | PCR chip with repeatedly used electrode |
| CN105296351A (en) * | 2015-12-02 | 2016-02-03 | 北京大学 | Chip for polymerase chain reaction (PCR), and real-time detection device and system |
| US10875026B2 (en) | 2016-02-22 | 2020-12-29 | Biofire Defense, Llc | Devices and methods for rapid PCR |
| EP3420107A4 (en) * | 2016-02-22 | 2019-07-31 | BioFire Defense, LLC | Devices and methods for rapid pcr |
| US11969731B2 (en) | 2016-02-22 | 2024-04-30 | Biofire Defense, Llc | Devices and methods for rapid PCR |
| CN108117983A (en) * | 2016-11-30 | 2018-06-05 | 希森美康株式会社 | Subject processing unit, subject processing method and subject processing chip |
| US11607684B2 (en) | 2016-12-19 | 2023-03-21 | Bforcure | Microfluidic sample chip, assay system using such a chip, and PCR method for detecting DNA sequences |
| CN110191759A (en) * | 2016-12-19 | 2019-08-30 | 碧佛尔酷瑞公司 | Micro-fluidic sample chip uses the analysis system of the chip and the PCR method for detecting DNA sequence dna |
| CN110452814A (en) * | 2019-07-12 | 2019-11-15 | 北京资和源医疗科技有限公司 | A kind of PCR fast reaction chip |
| WO2022246723A1 (en) * | 2021-05-27 | 2022-12-01 | 京东方科技集团股份有限公司 | Detection chip, and preparation method therefor and sample feeding method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102994369B (en) | 2014-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102994369B (en) | Chip structure for PCR (polymerase chain reaction) rapid reaction | |
| US11739383B2 (en) | Honeycomb tube | |
| EP2562247B1 (en) | Pcr device including two heating blocks | |
| US10413900B2 (en) | Microfluidic devices, systems and methods for sample preparation and analysis | |
| Liu et al. | DNA amplification and hybridization assays in integrated plastic monolithic devices | |
| CN203096061U (en) | Chip structure for PCR (polymerase chain reaction) fast reaction | |
| US9512421B1 (en) | Miniature acoustic wave lysis system and uses thereof | |
| CN107051598B (en) | PCR microfluidic chip, preparation and use methods thereof and PCR equipment | |
| Liang et al. | Fabrication of a microfluidic chip based on the pure polypropylene material | |
| CN111944682A (en) | Nucleic acid detection chip, preparation method and nucleic acid detection method | |
| Li et al. | PMMA microfluidic devices with three-dimensional features for blood cell filtration | |
| CN102899245A (en) | Micro-chamber static-state PCR (Polymerase Chain Reaction) and CE (Capillary Electrophoresis) function integrated micro-fluidic chip | |
| CN108277154B (en) | Spiral variable cross-section microfluidic PCR chip and manufacturing method thereof | |
| CN102033135A (en) | Integrated microfluidic chip interface, interface mould, and interface manufacturing and using methods | |
| Tai et al. | A novel integrated microfluidic platform to perform fluorescence in situ hybridization for chromosomal analysis | |
| CN202951486U (en) | Micro-fluidic chip integrating functions of micro-cavity dynamic polymerase chain reactions (PCRs) and capillary electrophoresis (CE) | |
| CN102899246A (en) | Dynamic PCR (Polymerase Chain Reaction) and CE (capillary electrophoresis) functional integrated micro-fluidic chip of microcavity | |
| CN218554115U (en) | Digital PCR chip and detection device based on nanogold heating | |
| US7398015B2 (en) | Apparatus for controlling fluid-heating using polymer disk | |
| CN110541023A (en) | digital PCR chip | |
| Zeng et al. | Thermal simulation for continuous-flow PCR system | |
| CN215196999U (en) | Film aluminum foil combined type micro-fluidic chip | |
| CN219507899U (en) | Multiple nest type nucleic acid amplification detector | |
| CN214120387U (en) | Enzyme reaction device with self-heating water supply | |
| US20240139746A1 (en) | Method and system for localized heating by illumination of patterned thin films |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice |
Addressee: Kay crystal Biotechnology (Suzhou) Co., Ltd. patent contacts Document name: Notification that Application Deemed not to be Proposed |
|
| ASS | Succession or assignment of patent right |
Owner name: ZHANG YINGPIN Free format text: FORMER OWNER: KAIJING BIOLOGICAL TECHNOLOGY (SUZHOU) CO., LTD. Effective date: 20140115 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 215123 SUZHOU, JIANGSU PROVINCE TO: 650200 KUNMING, YUNNAN PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20140115 Address after: 650200, No. 2, unit 5, 31 Tung Wan, Guandu District, Yunnan, Kunming Applicant after: Zhang Yingpin Address before: Suzhou City, Jiangsu province 215123 Park Linquan Street No. 399 Applicant before: Kaijing Biological Technology (Suzhou) Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160510 Address after: 230088 Customs Road, 669 hi tech Zone, Changjiang West Road, Anhui, Hefei K1 Patentee after: Anhui Anke Biotechnology (Group) Co., Ltd. Address before: 650200, No. 2, unit 5, 31 Tung Wan, Guandu District, Yunnan, Kunming Patentee before: Zhang Yingpin |