CN102993322A - Method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes - Google Patents
Method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes Download PDFInfo
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- CN102993322A CN102993322A CN2012104727548A CN201210472754A CN102993322A CN 102993322 A CN102993322 A CN 102993322A CN 2012104727548 A CN2012104727548 A CN 2012104727548A CN 201210472754 A CN201210472754 A CN 201210472754A CN 102993322 A CN102993322 A CN 102993322A
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Abstract
The invention relates to a method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes. The method comprises the following technical steps of: (1) drying and cold-pressing the tea cakes obtained after oil making; (2) crushing the cold-pressed tea cakes, and sieving by a sieve with 300 meshes; (3) extracting and centrifuging by adopting a supersonic wave-sodium hydroxide method to prepare the alkaline proteins and polysaccharides; (4) by taking residues of the extracted alkaline proteins and polysaccharides as raw materials, extracting and centrifuging to prepare acidic polysaccharose by adopting a supersonic wave-citric acid method; (5). centrifuging: to be specific, centrifuging at a high speed, wherein precipitations are the proteins, and a liquid supernatant is the polysaccharose; (6) purifying and drying; (7) detecting; and (8) packaging. The method has the advantages that 1. the synchronous extraction of the protein and the polysaccharide from the cold-pressed tea cakes is realized; 2. the yield of the proteins is increased to the maximum limit through the application of an alkaline process and an acid process; 3. the high additional value processing of the cold-pressed tea cakes is realized; and 4. the method is simple in productive technology and has wide application prospect.
Description
Technical field
The present invention relates to the method for a kind of tea cake protein, polysaccharide extraction, relate in particular to the method for a kind of cold press tea cake protein, polysaccharide simultaneous extraction.
Background technology
Tea oil has the laudatory title of " east sweet oil ", along with the attention of national forestry section and the human consumer increase in demand to product, in recent years, China's tea oil processing has obtained unprecedented development, wherein because the cold pressing oil technology has the processing conditions gentleness, form part and obtain maximum saving from damage, sex change does not occur in grouts, can be to again deep processing of grouts, so that this technology obtains the recognition and acceptance of processing enterprise.Therefore, the output of cold press tea cake is larger.The at present processing of cold press tea cake mainly contains direct extraction tea saponin, extracts polysaccharide, and wherein content all is not carried out utilization up to about 23% protein all the time, and cold press tea cake secondary industry benefit is not obvious, remains further technical research and process modification.
Summary of the invention
The object of the present invention is to provide the method for a kind of cold press tea cake protein, polysaccharide simultaneous extraction, successively take alkali lye, acid solution as extraction agent, ultrasonic-assisted extraction alkaline polysaccharide, protein, acidic polysaccharose are being realized protein, polysaccharide simultaneous extraction, high-valued, dark for cold press tea cake
New channel has been opened up in processing.
The present invention is achieved like this, and its processing step is:
1) drying: new cold press tea cake is regulated temperature 60 C-70 ℃ in loft drier, oven dry 4h;
2) pulverize: dried tea cake, adopt pulverizer and pulverize, cross 300 mesh sieves;
3) basic protein and polysaccharide extract: employing concentration is that the sodium hydroxide solution of 0.15mol/L-0.2 mol/L is extraction agent, and regulating pH is 8.5, solid-liquid ratio 1:20, and 80 ℃ of temperature, ultrasonic power is 80W, extracts 30min.
4) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min-3000r/min, and centrifugal 10min realizes separating of basic protein and polysaccharide soln;
5) acidic polysaccharose extracts: take the residue that extracts alkaline polysaccharide and protein as raw material, adopting concentration is that the citric acid solution of 0.5 mol/L-1 mol/L is extraction agent, and regulating pH is 3.0, solid-liquid ratio 1:20,80 ℃ of temperature, ultrasonic power 80w, extraction time 40min.
6) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min-3000r/min, and centrifugal 10min realizes that acidic polysaccharose solution separates with residue;
7) purifying: select respectively the film of molecular weight cut-off 10000Da to carry out separation and purification alkaline polysaccharide, acidic extraction liquid, trapped fluid is the polysaccharide extraction liquid of purifying;
8) drying: the protein after the centrifugation is regulated temperature 70 C-80 ℃ drying in loft drier, dry matter moisture is below 12%; Polysaccharide liquid behind the purifying is concentrated, and adjustable spraying tower inlet temperature is 190 ℃, and 80 ℃ of air outlet temperatures, flow velocity are 50ml/min, carry out drying.
9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
10) packing.
Advantage of the present invention is: the simultaneous extraction that 1, has realized cold press tea cake protein, polysaccharide; 2, the application of alkaline process, acid system has improved the yield of protein to greatest extent; 3, realized the processing of the high added value of cold press tea cake; 4, the production technique of present method is simple, application prospect is wide.
Embodiment
Embodiment 1
The present invention is achieved like this, and its processing step is:
1) drying: new cold press tea cake is regulated temperature 60 C in loft drier, oven dry 4h;
2) pulverize: dried tea cake, adopt pulverizer and pulverize, cross 300 mesh sieves;
3) basic protein and polysaccharide extract: employing concentration is that the sodium hydroxide solution of 0.15mol/L is extraction agent, and regulating pH is 8.5, solid-liquid ratio 1:20, and 80 ℃ of temperature, ultrasonic power is 80W, extracts 30min.
4) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min, and centrifugal 10min realizes separating of basic protein and polysaccharide soln;
5) acidic polysaccharose extracts: take the residue that extracts alkaline polysaccharide and protein as raw material, adopting concentration is that the citric acid solution of 0.5 mol/L is extraction agent, and regulating pH is 3.0, solid-liquid ratio 1:20,80 ℃ of temperature, ultrasonic power 80w, extraction time 40min.
6) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min, and centrifugal 10min realizes that acidic polysaccharose solution separates with residue;
7) purifying: select respectively the film of molecular weight cut-off 10000Da to carry out separation and purification alkaline polysaccharide, acidic extraction liquid, trapped fluid is the polysaccharide extraction liquid of purifying;
8) drying: the protein after the centrifugation is regulated temperature 70 C in loft drier dry, and dry matter moisture is below 12%; Polysaccharide liquid behind the purifying is concentrated, and adjustable spraying tower inlet temperature is 190 ℃, and 80 ℃ of air outlet temperatures, flow velocity are 50ml/min, carry out drying.
9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
10) packing.
Embodiment 2
The present invention is achieved like this, and its processing step is:
1) drying: new cold press tea cake is regulated temperature 70 C in loft drier, oven dry 4h;
2) pulverize: dried tea cake, adopt pulverizer and pulverize, cross 300 mesh sieves;
3) basic protein and polysaccharide extract: employing concentration is that the sodium hydroxide solution of 0.2 mol/L is extraction agent, and regulating pH is 8.5, solid-liquid ratio 1:20, and 80 ℃ of temperature, ultrasonic power is 80W, extracts 30min.
4) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 3000r/min, and centrifugal 10min realizes separating of basic protein and polysaccharide soln;
5) acidic polysaccharose extracts: take the residue that extracts alkaline polysaccharide and protein as raw material, adopting concentration is that the citric acid solution of 1 mol/L is extraction agent, and regulating pH is 3.0, solid-liquid ratio 1:20,80 ℃ of temperature, ultrasonic power 80w, extraction time 40min.
6) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 3000r/min, and centrifugal 10min realizes that acidic polysaccharose solution separates with residue;
7) purifying: select respectively the film of molecular weight cut-off 10000Da to carry out separation and purification alkaline polysaccharide, acidic extraction liquid, trapped fluid is the polysaccharide extraction liquid of purifying;
8) drying: the protein after the centrifugation is regulated 80 ℃ of dryings of temperature in loft drier, dry matter moisture is below 12%; Polysaccharide liquid behind the purifying is concentrated, and adjustable spraying tower inlet temperature is 190 ℃, and 80 ℃ of air outlet temperatures, flow velocity are 50ml/min, carry out drying.
9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
10) packing.
Embodiment 3
The present invention is achieved like this, and its processing step is:
1) drying: new cold press tea cake is regulated 65 ℃ of temperature in loft drier, oven dry 4h;
2) pulverize: dried tea cake, adopt pulverizer and pulverize, cross 300 mesh sieves;
3) basic protein and polysaccharide extract: employing concentration is that the sodium hydroxide solution of 0.18mol/L is extraction agent, and regulating pH is 8.5, solid-liquid ratio 1:20, and 80 ℃ of temperature, ultrasonic power is 80W, extracts 30min.
4) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2500r/min, and centrifugal 10min realizes separating of basic protein and polysaccharide soln;
5) acidic polysaccharose extracts: take the residue that extracts alkaline polysaccharide and protein as raw material, adopting concentration is that the citric acid solution of 0.6 mol/L is extraction agent, and regulating pH is 3.0, solid-liquid ratio 1:20,80 ℃ of temperature, ultrasonic power 80w, extraction time 40min.
6) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2500r/min, and centrifugal 10min realizes that acidic polysaccharose solution separates with residue;
7) purifying: select respectively the film of molecular weight cut-off 10000Da to carry out separation and purification alkaline polysaccharide, acidic extraction liquid, trapped fluid is the polysaccharide extraction liquid of purifying;
8) drying: the protein after the centrifugation is regulated 75 ℃ of dryings of temperature in loft drier, dry matter moisture is below 12%; Polysaccharide liquid behind the purifying is concentrated, and adjustable spraying tower inlet temperature is 190 ℃, and 80 ℃ of air outlet temperatures, flow velocity are 50ml/min, carry out drying.
9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
10) packing.
Claims (1)
1. the method for a cold press tea cake protein, polysaccharide simultaneous extraction is characterized in that processing step is:
1) drying: new cold press tea cake is regulated temperature 60 C-70 ℃ in loft drier, oven dry 4h;
2) pulverize: dried tea cake, adopt pulverizer and pulverize, cross 300 mesh sieves;
3) basic protein and polysaccharide extract: employing concentration is that the sodium hydroxide solution of 0.15mol/L-0.2 mol/L is extraction agent, and regulating pH is 8.5, solid-liquid ratio 1:20, and 80 ℃ of temperature, ultrasonic power is 80W, extracts 30min;
4) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min-3000r/min, and centrifugal 10min realizes separating of basic protein and polysaccharide soln;
5) acidic polysaccharose extracts: take the residue that extracts alkaline polysaccharide and protein as raw material, adopting concentration is that the citric acid solution of 0.5 mol/L is extraction agent, and regulating pH is 3.0, solid-liquid ratio 1:20,80 ℃ of temperature, ultrasonic power 80w, extraction time 40min;
6) centrifugal: with ultrasonic extraction liquid, rotating speed is controlled at 2000r/min-3000r/min, and centrifugal 10min realizes that acidic polysaccharose solution separates with residue;
7) purifying: select respectively the film of molecular weight cut-off 10000Da to carry out separation and purification alkaline polysaccharide, acidic extraction liquid, trapped fluid is the polysaccharide extraction liquid of purifying;
8) drying: the protein after the centrifugation is regulated temperature 70 C-80 ℃ drying in loft drier, dry matter moisture is below 12%; Polysaccharide liquid behind the purifying is concentrated, and adjustable spraying tower inlet temperature is 190 ℃, and 80 ℃ of air outlet temperatures, flow velocity are 50ml/min, carry out drying;
9) detect: the index of detection comprises physical and chemical index and biochemical indicator;
10) packing.
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| CN201210472754.8A CN102993322B (en) | 2012-11-21 | 2012-11-21 | Method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes |
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| CN201210472754.8A CN102993322B (en) | 2012-11-21 | 2012-11-21 | Method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes |
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| CN102993322A true CN102993322A (en) | 2013-03-27 |
| CN102993322B CN102993322B (en) | 2014-10-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107936131A (en) * | 2017-12-29 | 2018-04-20 | 贺州市星辉科技有限公司 | A kind of technique of acid-base method extraction Dendrobium officinale polysaccharide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817865A (en) * | 2010-04-20 | 2010-09-01 | 浙江大学 | Method for extracting and separating tea saponin and feeding proteoglycan by using residue-removed oil-tea camellia cake |
| CN101979400A (en) * | 2010-08-20 | 2011-02-23 | 上海师范大学 | Method for comprehensively extracting tea saponin and tea polysaccharide from camellia oil seed cake or tea seed cake |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817865A (en) * | 2010-04-20 | 2010-09-01 | 浙江大学 | Method for extracting and separating tea saponin and feeding proteoglycan by using residue-removed oil-tea camellia cake |
| CN101979400A (en) * | 2010-08-20 | 2011-02-23 | 上海师范大学 | Method for comprehensively extracting tea saponin and tea polysaccharide from camellia oil seed cake or tea seed cake |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107936131A (en) * | 2017-12-29 | 2018-04-20 | 贺州市星辉科技有限公司 | A kind of technique of acid-base method extraction Dendrobium officinale polysaccharide |
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