CN102987159B - Semi-artificial diet for plutella xylostella larvae, and preparation method and application of diet - Google Patents
Semi-artificial diet for plutella xylostella larvae, and preparation method and application of diet Download PDFInfo
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Abstract
The invention relates to semi-artificial diet for plutella xylostella larvae, and a preparation method and application of the diet. The semi-artificial diet for the plutella xylostella larvae is composed of the following components according to weight percentage: 2.67%-3.19% of malt flour, 1.07%-1.82% of cabbage powder, 3.21%-3.65% of cheese powder, 3.21%-3.65% of cane sugar powder, 0.86%-1.19% of Webster salt, 0.03%-0.05% of choline chloride, 0.09%-0.18% of sorbic acid, 0.32%-0.46% of ascorbic acid, 0.02%-0.05% of compound vitamin, 0.32%-0.46% of cholesterol, 2.14%-2.28% of agar, 0.49%-0.91% of glycerol, and the balance of water. The semi-artificial diet provided by the invention needs few raw materials which are easily available and is low in cost; the preparation method is simple and only comprises the step of boiling the materials to be dissolved; a feeding process with the diet is simple and is easy to operate; and the unit feeding quantity of the larvae is high, the development of the larvae is uniform, and thus the larvae is convenient for biological experiments such as bioassay.
Description
Technical field
The present invention relates to the man-made feeds of a kind of insect, refer to especially a kind of diamondback moth larvae meridic diet and its preparation method and application.
Background technology
Diamondback moth is the important pests of brassicaceous vegetable and crop.Raise the basis that neat diamondback moth larvae is the researchs such as diamondback moth ecology, toxicity, behavior, raw survey of growing in enormous quantities, at present domestic about being mainly to raise diamondback moth with wild cabbage or other natural hosts in the research of diamondback moth, natural host raising cost of labor is high; A small amount of research is to raise with man-made feeds, wherein import diamondback moth feed price costliness, and the arrival time is very long; And domestic known diamondback moth man-made feeds batching is many, process for preparation is still aobvious loaded down with trivial details, is difficult for commercialization, applies not extensive.Therefore still need diamondback moth artificial feeding further to research and develop.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of diamondback moth larvae meridic diet and its preparation method and application.
For achieving the above object, the invention provides a kind of diamondback moth larvae meridic diet, the each component of described diamondback moth larvae meridic diet and percentage by weight thereof are:
Malt flour: 2.67-3.19;
Wild cabbage powder: 1.07-1.82;
Cheese powder: 3.21-3.65;
Cane sugar powder: 3.21-3.65;
Webster salt: 0.86-1.19;
Choline Chloride: 0.03-0.05;
Sorbic acid: 0.09-0.18;
Ascorbic acid: 0.32-0.46;
B B-complex: 0.02-0.05;
Cholesterol: 0.32-0.46;
Agar: 2.14-2.28;
Glycerine: 0.49-0.91;
Water surplus.
The present invention also provides the preparation method of above-mentioned diamondback moth larvae meridic diet, described diamondback moth larvae meridic diet follows these steps to preparation: 1) weighed malt flour, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid, B B-complex, cholesterol, agar (each component effect: malt flour, wild cabbage powder, cheese powder, cane sugar powder are main nutrient composition, and carbohydrate, protein and dietary fiber are provided according to formula; Webster salt, Choline Chloride, ascorbic acid, B B-complex, cholesterol are auxiliary element, and it regulates the effect of metabolism, pre-disease prevention; Sorbic acid plays antisepsis; Agar plays freezing action), be evenly mixed into former powder; 2) hot water of then getting formula ratio adds wherein, add again the glycerine (glycerine plays moisture-keeping function) of formula ratio to stir, on electromagnetic oven, heat to 100 DEG C and boil, be distributed in multiple crispers, in each crisper, the thickness of the feed of pouring into is 5mm-10mm; 3) cover crisper mouth with gauze, and use the cardboard lid consistent with box open area to cover, after being condensed into bulk, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
As preferred version, wherein said wild cabbage powder is 60 DEG C of oven dry of cabbage leaves, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said malt flour be commercially available wheat berry vernalization to wheat self 1/2 length, after 60 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
As preferred version, the granularity of wherein said Choline Chloride was the particle of 16 mesh sieves.
As preferred version, the granularity of wherein said B B-complex is 60 orders (multivitamin and minerals tablet grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve).
As preferred version, the temperature of wherein said hot water is 90-100 DEG C.
As preferred version, the volume of wherein said crisper is 470 ~ 500cm
3.
The present invention further provides a kind of above-mentioned diamondback moth larvae meridic diet in the application of raising in diamondback moth larvae.
Diamondback moth larvae meridic diet composition must comprise the growth course desired nutritional material of diamondback moth, and meets the Basic Biological Characters such as the feeding habit of diamondback moth larvae.The feed of semi-artificial raising diamondback moth larvae reasonable in design should possess, necessary nutrition and reasonably nutritive proportion; There is stimulating appetite and be conducive to the composition that diamondback moth larvae is grown; There is the effective ingredient of preventing and curing diseases; There is good antiseptic effect.Nutrient balance is the key problem of development feed, and the imbalance of feed nutrition composition is grown the normal growth that affects diamondback moth larvae.
The growth course of diamondback moth larvae and life habit have uniqueness and the nutritional need of self, have significant difference with the insect of other artificial feeding in nutriment kind and ratio.
The composition the present invention is directed in feed has been researched and developed specific preparation method, and the physical behavior of the effective ingredient in feed and chemical analysis are protected to greatest extent.
The present invention has following beneficial effect:
1, meridic diet raw material is few, and source is easy to get, with low cost;
2, meridic diet preparation procedure is simple, only need get raw material and boil molten;
3, feeding process is simple, easy to operate;
4, unit raising worm amount is large, grows neatly, is convenient to raw survey and waits other biological test.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Diamondback moth larvae meridic diet is filled a prescription as shown in table 1, table 2.
Table 1: diamondback moth larvae meridic diet formula 1
Table 2: diamondback moth larvae meridic diet formula 2
Described diamondback moth larvae meridic diet follows these steps to preparation: 1) weighed malt flour, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid (also claiming vitamin C), B B-complex, cholesterol, agar according to formula, be evenly mixed into former powder; 2) hot water of then getting formula ratio adds wherein, add again the glycerine of formula ratio, stir, on electromagnetic oven, heat to 100 DEG C and boil, be distributed in crisper (its shape can be cylinder, square or cuboid etc.), in each crisper, the thickness of the feed of pouring into is 5mm-10mm; 3) cover crisper mouth with gauze, and use the cardboard lid consistent with box open area to cover, after being condensed into bulk, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
Described wild cabbage powder is wild cabbage (rich No. one of capital) 60 DEG C of oven dry of blade, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described malt flour is that commercially available wheat berry (kind is Zheng wheat 9023) vernalization, to wheat self 1/2 length, grinds after 60 DEG C of oven dry, crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
The granularity of described Choline Chloride was the particle (solid) of 16 mesh sieves.
The granularity of described B B-complex is that multivitamin and minerals tablet (also claiming Gold Theragran, Shanghai Gui Bao pharmaceutical Co. Ltd) grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
The temperature of described hot water is 90-100 DEG C.
The volume of described crisper is 470 ~ 500cm
3.
Table 3: diamondback moth larvae meridic diet formula and wild cabbage feeding effect contrast table (25 DEG C)
| Test feed combination | Survival rate | Percentage of pupation | Eclosion rate | Egg laying amount (grain/head) |
| Formula 1 | 83% | 85% | 92% | 180-210 |
| Formula 2 | 85% | 87% | 90% | 190-220 |
| Natural feed (wild cabbage) | 82% | 90% | 95% | 160-230 |
As can be seen from Table 3, under for examination borer population same case, raise diamondback moth larvae survival rates higher than natural feed (wild cabbage) 1-3% with diamondback moth larvae meridic diet formula 1 and formula 2, although percentage of pupation, eclosion rate are a little less than natural feed (wild cabbage) 3-5%, but all exceed 85%, its feeding effect and natural feed (wild cabbage) are raised quite.But diamondback moth larvae meridic diet formula is simple to operate, save cultured space, easily control, for the large-scale breeding of diamondback moth larvae provides indispensable condition.
These are only the preferred embodiment that the present invention is cited, not in order to limit the scope of the invention, the those of ordinary skill in affiliated technical field uses the equivalence that the present invention does modify or change, all with belonging to scope of patent protection of the present invention.
Claims (9)
1. a diamondback moth larvae meridic diet, is characterized in that, the each component of described diamondback moth larvae meridic diet and percentage by weight thereof are:
Malt flour: 2.67-3.19;
Wild cabbage powder: 1.07-1.82;
Cheese powder: 3.21-3.65;
Cane sugar powder: 3.21-3.65;
Webster salt: 0.86-1.19;
Choline Chloride: 0.03-0.05;
Sorbic acid: 0.09-0.18;
Ascorbic acid: 0.32-0.46;
B B-complex: 0.02-0.05;
Cholesterol: 0.32-0.46;
Agar: 2.14-2.28;
Glycerine: 0.49-0.91;
Water surplus;
Follow these steps to preparation: 1) weighed malt flour, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid, B B-complex, cholesterol, agar according to formula, be evenly mixed into former powder; 2) hot water of then getting formula ratio adds wherein, then adds the glycerine of formula ratio, stirs, and heats to 100 DEG C and boil on electromagnetic oven, is distributed in multiple crispers, and the thickness of pouring in each crisper is 5mm-10mm; 3) cover crisper mouth with gauze, and use the cardboard lid consistent with box open area to cover, after being condensed into bulk, put into 4 DEG C of refrigerators, refrigerate for subsequent use.
2. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, described wild cabbage powder is 60 DEG C of oven dry of cabbage leaves, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
3. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, described malt flour be commercially available wheat berry vernalization to wheat self 1/2 length, after 60 DEG C of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
4. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, the granularity of described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
5. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve; Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
6. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, described Choline Chloride was the particle of 16 mesh sieves; Described B B-complex is that multivitamin and minerals tablet grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
7. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, the volume of described crisper is 470 ~ 500cm
3.
8. diamondback moth larvae meridic diet as claimed in claim 1, is characterized in that, the temperature of described hot water is 90-100 DEG C.
9. a diamondback moth larvae meridic diet claimed in claim 1 is in the application of raising in diamondback moth larvae.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103478486A (en) * | 2013-09-11 | 2014-01-01 | 华南农业大学 | Diamondback moth larva pure artificial feed and preparation method thereof |
| CN105010243B (en) * | 2015-07-28 | 2017-06-16 | 武汉科诺生物科技股份有限公司 | A kind of diamondback moth man-made feeds and diamondback moth indoors artificial method for breeding |
| CN105146229A (en) * | 2015-10-30 | 2015-12-16 | 湖北省农业科学院植保土肥研究所 | Rice stem borer larva artificial feed and preparation method and application thereof |
| CN116098248A (en) * | 2023-02-16 | 2023-05-12 | 福建省农业科学院植物保护研究所 | Compound vitamin feed additive for lepidopteran insect larvae |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3974171A (en) * | 1973-10-09 | 1976-08-10 | Bayer Aktiengesellschaft | O-[3-methyl-1,3,4-triazole-(2,3,-b)-thiazol(6)yl]-(thiono)-phosphoric(phosphonic) acid esters |
| CN101347184A (en) * | 2008-09-09 | 2009-01-21 | 云南省农业科学院农业环境资源研究所 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3974171A (en) * | 1973-10-09 | 1976-08-10 | Bayer Aktiengesellschaft | O-[3-methyl-1,3,4-triazole-(2,3,-b)-thiazol(6)yl]-(thiono)-phosphoric(phosphonic) acid esters |
| CN101347184A (en) * | 2008-09-09 | 2009-01-21 | 云南省农业科学院农业环境资源研究所 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
Non-Patent Citations (1)
| Title |
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| 小菜蛾群体繁殖技术;陈宗麒，等;《昆虫知识》;20011231;第38卷(第1期);第68-70页 * |
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