CN102972300A - Oat regeneration system construction method - Google Patents
Oat regeneration system construction method Download PDFInfo
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- CN102972300A CN102972300A CN2012105290533A CN201210529053A CN102972300A CN 102972300 A CN102972300 A CN 102972300A CN 2012105290533 A CN2012105290533 A CN 2012105290533A CN 201210529053 A CN201210529053 A CN 201210529053A CN 102972300 A CN102972300 A CN 102972300A
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Abstract
The invention discloses an oat regeneration system construction method. According to the oat regeneration system construction method, leaf base section cultivation is adopted, and differentiation seedling is directly formed without callus; and the method comprises the following steps of: 1, sterilizing mature oat seeds, and culturing for 4-7 days by using an MS minimal medium; and 2, taking the leaf base section to culture to form seedlings through an MSB culture medium when the oat leaf grows to 3-5cm. The invention provides the oat regeneration system construction method which aims at optimizing an oat regeneration system, takes the operation of keeping the hereditary stability as a core, and overcomes the defects in the conventional culture mode on the base of improving the tissue culture planting percent.
Description
Technical field
The invention belongs to oat regenerating system construction method.
Background technology
The plant regeneration system construction is to utilize totipotency of plant cell, under aseptic condition, utilize synthetic medium to the cultivation of plant tissue, being that rareness species keeps, the important channel of breeding, also is the basis that detoxic seedling is produced in batch production, especially the necessary links of gene function checking.
The present regenerating system construction method of oat mainly will have callus cultivation, the tip of a root, anther culture etc.The main explant that callus is cultivated is immature embryo and mature embryo.Immature embryo culture and Mature embryo culture healing rate are high, but its same space-time that exists with anther culture, must etc. during the thin female born of the same parents' maturation of vegetalitas or rataria begin to break up and just can draw materials.And gramineous plants root tip culture and anther culture also will be induced the generation callus, seedling behind Calli Differentiation.And derive from the callus of different plants or same plant different parts, and all may there are differences in color, structure, habit of growth etc., therefore need to be according to the state of different experiment purpose control callus.This is to strict such as: preparation of temperature, illumination, pH value, medium etc. of explant, experiment condition, and the structure of every kind of cultivating system all needs to grope for a long time, expends a large amount of human and material resources.And in the callus incubation, because the vigorous as easy as rolling off a log generation of the division of cell becomes different, and cause the incident factor of change a lot, as: illumination, temperature, medicine, ultraviolet ray etc., this is unfavorable for the checking of gene function very much, also is unfavorable for the maintenance of plant genetic stability.
Summary of the invention
The present invention is to optimize the oat regenerating system as purpose; To keep genetic stability as core, cultivate planting percent from improving tissue, solve the drawback of above-mentioned existing training method, a kind of construction method of oat regenerating system is provided.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of oat regenerating system construction method, described oat regenerating system construction method adopt the phyllopodium section to cultivate, and without the direct seedling differentiation of callus, comprise the steps:
S1, with after the sterilization of ripe oat seed, cultivated 4-7 days with the MS minimal medium;
S2, when the oat leaf grows to 3-5cm, get the phyllopodium section with the MSB medium culture to seedling.
Preferably, it is to get its base segment behind the seed germination to cultivate that described phyllopodium section is cultivated, and directly differentiation is taken root downwards, upwards comes into leaves.
Preferably, described MSB medium is that MS combines with B5, adds IAA and caseinhydrolysate.
Adopted technique scheme, beneficial effect of the present invention is: the present invention without the callus stage, does not need subculture to cultivate because adopting the phyllopodium section to cultivate, and has avoided issuable gene mutation in the callus incubation; Do not need to monitor the state of callus, needn't grope the subculture condition of culture, reduced experiment link, save human and material resources, significantly reduce production costs, improve output benefit.Through experiment test, regenerating system method planting percent of the present invention reaches more than 98%, compares with existing callus culture method, saves time 3 to 6 months, and every liter of culture medium cost reduces by 0.11 to 4.68 yuan.
Description of drawings
Fig. 1 is the schematic diagram of seed sprouting;
Fig. 2 is the schematic diagram that the phyllopodium section is cultivated;
Fig. 3 is the schematic diagram of seedling differentiation;
Embodiment
The present invention is further described below in conjunction with executing example.
Implementation condition of the present invention: aseptic condition, Culture Medium Laboratory, clean workbench is grasped by temperature, illumination, the controlled culturing room of humidity.
Essence of the present invention is: the phyllopodium section is without callus but directly produce indefinite bud, Germination And Seedling.
A kind of oat regenerating system construction method, described oat regenerating system construction method adopt the phyllopodium section to cultivate, and without the direct seedling differentiation of callus, comprise the steps:
S1, with after the sterilization of ripe oat seed, cultivated 4-7 days with the MS minimal medium;
S2, when the oat leaf grows to 3-5cm, get the phyllopodium section with the MSB medium culture to seedling.
It is to get its base segment behind the seed germination to cultivate that the phyllopodium section is cultivated, and directly differentiation is taken root downwards, upwards comes into leaves.
The MSB medium is that MS combines with B5, adds IAA and caseinhydrolysate.
The present invention is not limited to above-mentioned concrete embodiment, and those of ordinary skill in the art is from above-mentioned design, and without performing creative labour, all conversion of having done all drop within protection scope of the present invention.
Claims (3)
1. an oat regenerating system construction method is characterized in that, described oat regenerating system construction method adopts the phyllopodium section to cultivate, and without the direct seedling differentiation of callus, comprises the steps:
S1, with after the sterilization of ripe oat seed, cultivated 4-7 days with the MS minimal medium;
S2, when the oat leaf grows to 3-5cm, get the phyllopodium section with the MSB medium culture to seedling.
2. a kind of oat regenerating system construction method as claimed in claim 1 is characterized in that, it is to get its base segment behind the seed germination to cultivate that described phyllopodium section is cultivated, and directly differentiation is taken root downwards, upwards comes into leaves.
3. such as right a kind of oat regenerating system construction method as claimed in claim 1 or 2, it is characterized in that described MSB medium is that MS combines with B5, adds IAA and caseinhydrolysate.
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| CN2012105290533A CN102972300A (en) | 2012-12-02 | 2012-12-02 | Oat regeneration system construction method |
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| CN2012105290533A CN102972300A (en) | 2012-12-02 | 2012-12-02 | Oat regeneration system construction method |
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| CN102972300A true CN102972300A (en) | 2013-03-20 |
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| CN2012105290533A Pending CN102972300A (en) | 2012-12-02 | 2012-12-02 | Oat regeneration system construction method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027193A (en) * | 2021-11-15 | 2022-02-11 | 吉林省白城市农业科学院(吉林省向日葵研究所) | Culture medium for improving callus induction rate of oat anther culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999015003A1 (en) * | 1997-09-24 | 1999-04-01 | The Regents Of The University Of California | Methods and compositions for transformation of cereals using cultured shoot meristematic tissue |
| CN1580257A (en) * | 2003-08-13 | 2005-02-16 | 中国科学院植物研究所 | Method for breeding transgenic wheat and its use |
| CN1596617A (en) * | 2004-08-06 | 2005-03-23 | 山东大学 | Method of establishing early-maturing ripe hereditary transform system and application |
| CN102352378A (en) * | 2011-10-23 | 2012-02-15 | 成都大学 | Genetic transformation method of tartary buckwheat lamina |
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2012
- 2012-12-02 CN CN2012105290533A patent/CN102972300A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999015003A1 (en) * | 1997-09-24 | 1999-04-01 | The Regents Of The University Of California | Methods and compositions for transformation of cereals using cultured shoot meristematic tissue |
| CN1580257A (en) * | 2003-08-13 | 2005-02-16 | 中国科学院植物研究所 | Method for breeding transgenic wheat and its use |
| CN1596617A (en) * | 2004-08-06 | 2005-03-23 | 山东大学 | Method of establishing early-maturing ripe hereditary transform system and application |
| CN102352378A (en) * | 2011-10-23 | 2012-02-15 | 成都大学 | Genetic transformation method of tartary buckwheat lamina |
Non-Patent Citations (1)
| Title |
|---|
| 杨才等: "用未成熟胚离体培养法获得四倍体大燕麦与六倍体裸燕麦杂种植株的研究初报", 《麦类作物》, vol. 18, no. 5, 30 September 1998 (1998-09-30) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027193A (en) * | 2021-11-15 | 2022-02-11 | 吉林省白城市农业科学院(吉林省向日葵研究所) | Culture medium for improving callus induction rate of oat anther culture |
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Application publication date: 20130320 |