CN102948475A - Preparation method for probiotics milk powder - Google Patents
Preparation method for probiotics milk powder Download PDFInfo
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- CN102948475A CN102948475A CN2012105071748A CN201210507174A CN102948475A CN 102948475 A CN102948475 A CN 102948475A CN 2012105071748 A CN2012105071748 A CN 2012105071748A CN 201210507174 A CN201210507174 A CN 201210507174A CN 102948475 A CN102948475 A CN 102948475A
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Abstract
The invention discloses a preparation method of probiotics milk powder. The preparation method comprises the following steps: 1), raw milk treatment: carrying out an ultra-temperature instant sterilization treatment to on the raw milk and then carrying out vacuum depression concentration to obtain concentrated milk; 2), bacteria cultivation: inoculating probiotics to sterilized fresh milk or enrichment medium to obtain a bacteria nutrient solution, wherein the volume of the inoculated probiotics is 1-3% of the volume of the bacteria nutrient solution; 3), homogenization: adding 1-10% of bacteria culture in percentage by weightmass fraction to the concentrated milk in the step 1) for homogenizing to obtain a concentrated milk mixture containing probiotics; and 4), spraying and drying the concentrated milk mixture containing the probiotics to obtain the milk powder containing probiotics. The preparation method disclosed by the invention has a simple operation process, low device requirements, saves cost and is suitable for large-scale production. The probiotics milk powder prepared from by the preparation method provided by the invention has all nutrition of common milk powder, and further has health benefits of various probiotics.
Description
Technical field
The invention belongs to food processing field, be specifically related to a kind of preparation method of probio milk powder.
Background technology
At present, the health efficacy of probio is confirmed the approval with the consumer fully, but also is constantly disclosing its new effect to the human body beneficial.The application share of probio in dairy products is maximum, various in style, is mainly the liquid diary products such as probio yogurt and sour milk beverage, liked by the consumer.But viable bacteria maintenance phase in the liquid diary product is short, under 0 ℃～8 ℃, and one week of yogurt, sour milk beverage 1～3 month, and the stage such as preserving, transport, sell, drink, potential safety hazard happens occasionally, and causes the health efficacy of probio to can not get real performance.Keep the phase in order to prolong viable bacteria, the method of mainly taking at present mainly is to add the additives such as high-concentration bacterial powder, anticorrisive agent, perhaps use extra package, technique carried out the measures such as specially treated, so that original local flavor reduction of probio, health effect can not get fully playing and embodying, and cost significantly increases.In addition, in the market, external import bacterial classification has occupied leading position for a long time, domestic probio dairy products mouthfeel, local flavor and performance all are affected, cause selling point limited, the market competitiveness can't ensure, makes the development of China's yogurt and active probiotic dairy products industry thereof be in bottleneck.
Milk powder is the desirable carrier of probio, but bacterium is impaired larger during drying of milk powder, and therefore, research focus still concentrates on and improves on the thalline vigor at present.Existing probio milk powder nearly all is imported product on the market, makes by adding the freeze-dried vaccine powder, and cost is high, technology dependence is strong, lack competitiveness, and the bacterium powder is in product during secondary operations, and microbial activity reduces significantly.
Summary of the invention
The problem that the present invention solves is to provide a kind of preparation method of probio milk powder, and it is low to solve in the prior art in the probiotic bacteria milk product viable count, and the bacterium survival period is short, uses additive to change the original local flavor of probio, is not easy to the problems such as transportation, storage.
The present invention is achieved through the following technical solutions:
A kind of preparation method of probio milk powder may further comprise the steps:
(1) will carry out vacuum-concentrcted through the raw milk that ultra high temperature short time sterilization is processed and obtain concentrated breast;
(2) probio is inoculated in the sweet milk or enriched medium after the sterilization, then 37～45 ℃ of lower constant temperature culture, obtains bacterial culture fluid, again 4 ℃ lower centrifugal, collecting precipitation obtains the probio thalline;
In mass fraction, described enriched medium consist of 8～10 parts of whey powders, yeast soaks 3～5 parts in powder, 20～25 parts of cornstarch, 100～300 parts of nutritional extracts, 1000 parts of distilled water;
Described nutritional extract is one or more of cucumber, ternip, carrot or Chinese yam medicinal extract;
(3) the probio thalline is made an addition to concentrated Ruzhong by mass fraction 1～10%, and add protective agent, obtain containing the concentrated newborn mixture of profitable probliotics behind the homogeneous;
(4) will contain the concentrated newborn mixture of profitable probliotics, the spray-dried milk powder that obtains containing profitable probliotics.
Described ultra high temperature short time sterilization is to carry out under 120～150 ℃ 1～2 second;
Described vacuum-concentrcted pressure process is 15.33～16.00 * 10
3Pa, temperature is 51～56 ℃.
In the described enriched medium, the volume of inoculation probio accounts for 1～3% of bacterial culture fluid volume.
The preparation process of described cucumber, ternip, carrot or Chinese yam medicinal extract is:
With fresh cucumber, ternip, carrot or Chinese yam, add the 5～10min that pulls an oar in the mixer with water by the mass ratio of 1:1～2, after boiling 5～10min, cool off in the slurries that making beating is obtained, filter, obtain nutritional extract after collecting the filtrate drying.
The sterilization process of described sweet milk or enriched medium: sweet milk is at 115 ℃ of sterilization 10min, and enriched medium is at 115 ℃ of sterilization 20min.
The time of described constant temperature culture is: need be cultured to curdled milk in sweet milk, cultivate 12h～48h in enriched medium.
Described protective agent comprises leucine, Cobastab
12And glutamic acid, its mass concentration at the concentrated newborn mixture that contains profitable probliotics is respectively: leucine 0.01～0.02%, Cobastab
120.005～0.01% and glutamic acid 0.02～0.04%.
Described homogenizing process temperature is 45～50 ℃, and pressure remains on 20～25MPa.
The described EAT that spray dryer is set is 130～150 ℃, 70～85 ℃ of tower temperature, 70～85 ℃ of leaving air temps.
Compared with prior art, the present invention has following useful technique effect:
The preparation method of the probio milk powder that this method provides; probio is inoculated in carries out constant temperature culture in the culture medium; in order to guarantee that probio has higher viable count; and according to its growth metabolism characteristic and nutritional need add nutritional extract carry out High Density Cultivation, cultivate finish after the interpolation protective agent thalline is protected; behind the thalline culture and the concentrated newborn homogeneous of raw material that obtain, adopt spray drying process to prepare probio milk powder.
The preparation method of the probio milk powder that this method provides, step is simple, and is easy and simple to handle, low for equipment requirements, can effectively reduce cost, is fit to extensive the amplification and produces.
The preparation method of the probio milk powder that this method provides, the bacterium of probio activity is high in the prepared probio milk powder, survival period is long, and this milk powder also has the health efficacy of multiple probio except the full nutrition with ordinary powdered milk.Can directly reconstitute and drink, can also add the auxiliary materials such as fruits and vegetables cereal according to the personal like, now lead to yogurt and the sour milk beverage of different mouthfeels and local flavor.Have nutrition, health, safety, advantage easily.
The specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment, and the explanation of the invention is not limited.
The probio bacterial classification that this method adopts is the probio that can be used for health food through the health ministry approval.
Embodiment 1
The preparation method of probio milk powder may further comprise the steps:
(1) processing of raw milk: raw milk (cow's milk) was carried out ultra high temperature short time sterilization 1～2 second under 135 ℃ of conditions; Then, reduced pressure concentration in vacuum concentration pot, vacuum keep is 15.67 * 10
3Pa, temperature maintains 51 ℃, obtains concentrated breast after concentrating.
(2) cultivation of thalline: 3ml probio (streptococcus thermophilus) is inoculated under 115 ℃ of conditions in the 300ml sweet milk behind the sterilization 10min, and 37 ℃ of constant temperature culture are to curdled milk.
(3) with the curdled milk that obtains at 4 ℃, centrifugal 10min under the condition of 6000r/min collects the probio thalline;
The probio thalline culture that obtains is made an addition to concentrated Ruzhong by the amount of mass fraction 4%, add protective agent, make to comprise leucine, Cobastab
12Be respectively with the mass concentration of glutamic acid: leucine 0.01%, Cobastab
120.005% and glutamic acid 0.04%; At 50 ℃, carry out homogeneous under the pressure 22MPa condition, obtain containing the concentrated newborn mixture of profitable probliotics.
(4) spray-drying: the concentrated newborn mixture that contains profitable probliotics is carried out spray-drying, and the EAT that spray dryer is set is 140 ℃, 70 ℃ of tower temperature, 75 ℃ of leaving air temps.
Can obtain to contain the milk powder of profitable probliotics after the spray-drying.
Embodiment 2
The preparation method of probio milk powder may further comprise the steps:
(1) processing of raw milk: raw milk was carried out ultra high temperature short time sterilization 1～2 second under 120 ℃ of conditions; Then, reduced pressure concentration in vacuum concentration pot immediately, vacuum keep is 16.00 * 10
3Pa, temperature maintains 53 ℃, namely gets concentrated breast after concentrating, and milk solids content is 40～50%.
(2) cultivation of thalline: prepare first enriched medium, with whey powder 10g, yeast soaks powder 5g, cornstarch 25g, and cucumber medicinal extract 180g is dissolved in the 1000ml distilled water, abundant mixing, 20min sterilizes under 115 ℃ of conditions;
With 10ml probio bacterial classification (streptococcus thermophilus and lactobacillus bulgaricus, 1:1 mixes by volume) be inoculated in the above-mentioned enriched medium, 38 ℃ of constant temperature culture 32h, after obtaining bacterial culture fluid, with this bacterial culture fluid at 4 ℃, centrifugal 10min under the 6000r/min condition collects and obtains the probio thalline.
(3) homogeneous: the probio thalline that obtains is made an addition to concentrated Ruzhong by mass fraction 7%, add protective agent, the mass concentration that comprises leucine, cobalamin and glutamic acid is respectively: leucine 0.02%, cobalamin 0.006% and glutamic acid 0.02%; At 48 ℃, carry out homogeneous under the pressure 20MPa condition, obtain containing the concentrated newborn mixture of profitable probliotics.
(4) spray-drying: behind the homogeneous, the concentrated newborn mixture that contains profitable probliotics is carried out spray-drying, the EAT that spray dryer is set is 130 ℃, 70 ℃ of tower temperature, 70 ℃ of leaving air temps.
Can obtain to contain the milk powder of profitable probliotics after the spray-drying.
Embodiment 3
The preparation method of probio milk powder may further comprise the steps:
(1) processing of raw milk: raw milk was carried out ultra high temperature short time sterilization 1～2 second under 150 ℃ of conditions; Then, reduced pressure concentration in vacuum concentration pot immediately, vacuum keep is 15.45 * 10
3Pa, temperature maintains 55 ℃, namely gets concentrated breast after concentrating, and milk solids content is 40～50%.
(2) cultivation of thalline: prepare first enriched medium, with whey powder 10g, yeast soaks powder 5g, cornstarch 25g, and the mixture 100g of ternip medicinal extract and carrot medicinal extract is dissolved in the 1000ml distilled water, abundant mixing, 20min sterilizes under 115 ℃ of conditions;
10ml probio bacterial classification (lactobacillus bulgaricus) is inoculated in the above-mentioned enriched medium, 37 ℃ of constant temperature culture 48h, obtain bacterial culture fluid after, at 4 ℃, centrifugal 10min under the 6000r/min condition collects and to obtain the probio thalline with the thalline culture.
(3) homogeneous: the probio thalline that obtains is made an addition to concentrated Ruzhong by mass fraction 1%, add protective agent, the mass concentration that comprises leucine, cobalamin and glutamic acid is respectively: leucine 0.03%, cobalamin 0.008% and glutamic acid 0.025%; At 47 ℃, carry out homogeneous under the pressure 25MPa condition, obtain containing the concentrated newborn mixture of profitable probliotics.
(4) spray-drying: behind the homogeneous, the concentrated newborn mixture that contains profitable probliotics is carried out spray-drying, the EAT that spray dryer is set is 150 ℃, 79 ℃ of tower temperature, 80 ℃ of leaving air temps.Can obtain to contain the milk powder of profitable probliotics after the spray-drying.
Embodiment 4
The preparation method of probio milk powder may further comprise the steps:
(1) processing of raw milk: raw milk is carried out ultra high temperature short time sterilization, 1～2 second under 140 ℃ of conditions; Then, reduced pressure concentration in vacuum concentration pot immediately, vacuum keep is 15.33 * 10
3Pa, temperature maintains 56 ℃, namely gets concentrated breast after concentrating, and milk solids content is 40～50%.
(2) cultivation of thalline: prepare first enriched medium, with whey powder 10g, yeast soaks powder 5g, cornstarch 25g, and the mixture 300g of Chinese yam medicinal extract and cucumber medicinal extract is dissolved in the 1000ml distilled water, abundant mixing, 20min sterilizes under 115 ℃ of conditions;
With 10ml probio bacterial classification (streptococcus thermophilus and lactobacillus bulgaricus, 1:1.2 mixes by volume) be inoculated in the above-mentioned enriched medium, 45 ℃ of constant temperature culture 12h, after obtaining bacterial culture fluid, with the thalline culture at 4 ℃, centrifugal 10min under the 6000r/min condition collects and obtains the probio thalline.
(3) homogeneous: the probio thalline that obtains that will obtain makes an addition to concentrated Ruzhong by mass fraction 10%, add protective agent, the mass concentration that comprises leucine, cobalamin and glutamic acid is respectively: leucine 0.025%, cobalamin 0.01% and glutamic acid 0.03%; At 45 ℃, carry out homogeneous under the pressure 23MPa condition, obtain containing the concentrated newborn mixture of profitable probliotics.
(4) spray-drying: behind the homogeneous, the concentrated newborn mixture that contains profitable probliotics is carried out spray-drying, the EAT that spray dryer is set is 145 ℃, 85 ℃ of tower temperature, 85 ℃ of leaving air temps.Can obtain to contain the milk powder of profitable probliotics after the spray-drying.
Claims (9)
1. the preparation method of a probio milk powder is characterized in that, may further comprise the steps:
(1) will carry out vacuum-concentrcted through the raw milk that ultra high temperature short time sterilization is processed and obtain concentrated breast;
(2) probio is inoculated in the sweet milk or enriched medium after the sterilization, obtains bacterial culture fluid; After 37～45 ℃ of lower constant temperature culture, centrifugal under 4 ℃, collecting precipitation obtains the probio thalline with bacterial culture fluid;
In mass fraction, described enriched medium consist of 8～10 parts of whey powders, yeast soaks 3～5 parts in powder, 20～25 parts of cornstarch, 100～300 parts of nutritional extracts, 1000 parts of distilled water;
Described nutritional extract is one or more of cucumber, ternip, carrot or Chinese yam medicinal extract;
(3) the probio thalline is made an addition to concentrated Ruzhong by mass fraction 1～10%, and add protective agent, obtain containing the concentrated newborn mixture of profitable probliotics behind the homogeneous;
(4) will contain the concentrated newborn mixture of profitable probliotics, the spray-dried milk powder that obtains containing profitable probliotics.
2. the preparation method of a kind of probio milk powder according to claim 1, it is characterized in that: the described ultra high temperature short time sterilization of step 1) is to carry out under 120～150 ℃ 1～2 second;
Described vacuum-concentrcted pressure process is 15.33～16.00 * 10
3Pa, temperature is 51～56 ℃.
3. the preparation method of a kind of probio milk powder according to claim 1 is characterized in that: step 2) in the described enriched medium, the volume of inoculation probio accounts for 1～3% of bacterial culture fluid volume.
4. the preparation method of a kind of probio milk powder according to claim 1, it is characterized in that: the preparation process of described cucumber, ternip, carrot or Chinese yam medicinal extract is:
With fresh cucumber, ternip, carrot or Chinese yam, add the 5～10min that pulls an oar in the mixer with water by the mass ratio of 1:1～2, after boiling 5～10min, cool off in the slurries that making beating is obtained, filter, obtain nutritional extract after collecting the filtrate drying.
5. the preparation method of a kind of probio milk powder according to claim 1 is characterized in that: step 2) sterilization process of described sweet milk or enriched medium: sweet milk is at 115 ℃ of sterilization 10min, and enriched medium is at 115 ℃ of sterilization 20min.
6. the preparation method of a kind of probio milk powder according to claim 1 is characterized in that: step 2) time of described constant temperature culture is: in sweet milk, need be cultured to curdled milk, in enriched medium, cultivate 12h～48h.
7. the preparation method of a kind of probio milk powder according to claim 1, it is characterized in that: described protective agent comprises leucine, Cobastab
12And glutamic acid, its mass concentration at the concentrated newborn mixture that contains profitable probliotics is respectively: leucine 0.01～0.02%, Cobastab
120.005～0.01% and glutamic acid 0.02～0.04%.
8. the preparation method of a kind of probio milk powder according to claim 1, it is characterized in that: the described homogenizing process temperature of step 3) is 45～50 ℃, and pressure remains on 20～25MPa.
9. the preparation method of a kind of probio milk powder according to claim 1, it is characterized in that: the EAT that step 4) arranges spray dryer is 130～150 ℃, 70～85 ℃ of tower temperature, 70～85 ℃ of leaving air temps.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103478530A (en) * | 2013-09-02 | 2014-01-01 | 陕西科技大学 | Oligosaccharide probiotics composition and application thereof as well as method for preparing capsules by using composition |
| CN103493884A (en) * | 2013-09-02 | 2014-01-08 | 陕西科技大学 | Preparation method of instant-brew-yogurt milk powder |
| CN105852082A (en) * | 2016-05-04 | 2016-08-17 | 张树合 | Milk yeast and preparation method thereof |
| CN109463453A (en) * | 2018-10-29 | 2019-03-15 | 东阿阿胶股份有限公司 | A kind of preparation method of probiotics donkey milk powder |
| CN111990459A (en) * | 2020-08-21 | 2020-11-27 | 江苏省农业科学院 | Burdock probiotic milk powder for improving activity of probiotics and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478530A (en) * | 2013-09-02 | 2014-01-01 | 陕西科技大学 | Oligosaccharide probiotics composition and application thereof as well as method for preparing capsules by using composition |
| CN103493884A (en) * | 2013-09-02 | 2014-01-08 | 陕西科技大学 | Preparation method of instant-brew-yogurt milk powder |
| CN103493884B (en) * | 2013-09-02 | 2014-12-03 | 陕西科技大学 | Preparation method of instant-brew-yogurt milk powder |
| CN105852082A (en) * | 2016-05-04 | 2016-08-17 | 张树合 | Milk yeast and preparation method thereof |
| CN109463453A (en) * | 2018-10-29 | 2019-03-15 | 东阿阿胶股份有限公司 | A kind of preparation method of probiotics donkey milk powder |
| CN111990459A (en) * | 2020-08-21 | 2020-11-27 | 江苏省农业科学院 | Burdock probiotic milk powder for improving activity of probiotics and preparation method thereof |
| CN111990459B (en) * | 2020-08-21 | 2023-08-18 | 江苏省农业科学院 | Burdock probiotic milk powder for improving activity of probiotics and preparation method thereof |
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Application publication date: 20130306 |