CN102944550B - Detection kit for calcium peroxide in flour and detection method for calcium peroxide - Google Patents
Detection kit for calcium peroxide in flour and detection method for calcium peroxide Download PDFInfo
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- CN102944550B CN102944550B CN201210388832.6A CN201210388832A CN102944550B CN 102944550 B CN102944550 B CN 102944550B CN 201210388832 A CN201210388832 A CN 201210388832A CN 102944550 B CN102944550 B CN 102944550B
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- calcium peroxide
- calper calcium
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Abstract
The present invention provides a detection kit for calcium peroxide in flour, which is composed of plastic sample spoons, plastic droppers, test tubes, a measuring cylinder, a test tube rack, a standard colorimetric card, a chromogenic reagent, pH test strips, reaction reagents, and a wash bottle. Tests are carried out on the samples by observing the different colors being shown and contrasting with the standard colorimetric card to perform qualitative and semi-quantitative detections. The detection method for calcium peroxide in flour includes: adding dilute acid into flour to react, adjusting the pH value to neutral with an alkaline solution, adding a mixed solution including a peroxidase-containing reagent, developing color, comparing with the standard colorimetric card to obtain the qualitative and semi-quantitative result. The detection kit and the detection method of the invention have the advantages of easy usage, fast color developing, relatively loose detection conditions, high sensitivity and low cost, and are suitable for on-site rapid screening assays.
Description
Technical field
The present invention relates to detection kit and application thereof, especially relate to the kit of calper calcium peroxide in a kind of Fast Measurement flour and the assay method to admixture calper calcium peroxide quantitative and semi-quantitative artificial in flour.
Background technology
Calper calcium peroxide is light yellow crystalline powder, molecular weight 72.08, is widely used in the industries such as food, chemical industry, weaving.The fields such as the after-ripening, the flour that are usually used in improving wheat flour brighten, flour gluten fortifier, flour decolorant, bread improver, edible oil bleaching.In order to increase chewiness and the mouthfeel of wheat flour, flour industry generally adds benzoyl peroxide, calper calcium peroxide as wheat flour gluten fortifier, whitening agent.China, from 1 day May in 2011, forbids adding benzoyl peroxide, calper calcium peroxide [Ministry of Public Health etc. seven (office) issue " bulletin about cancelling food additives benzoyl peroxide, calper calcium peroxide " (2011 No. 4)] in Flour production.
Added to by calper calcium peroxide in flour, long-term eating may be carcinogenic, can produce very large harm to health.And some Flour production producer is in order to flour decolouring, the slaking accelerating flour, benzoyl peroxide or calper calcium peroxide may be added excessively in violation of rules and regulations.In existing national standard, industry standard and open source literature, also do not detect method and the quick measuring reagent box of calper calcium peroxide in flour.Therefore, set up one fast and accurately in flour calper calcium peroxide qualitative and semiquantitative determination method is significant.
Summary of the invention
The object of the invention is to provide a kind of kit detected fast calper calcium peroxide in flour, and applies this kit carries out quantitative and semi-quantitative assay method to calper calcium peroxide in flour.For this reason, the present invention adopts following technical scheme:
In flour, calper calcium peroxide quick measuring reagent box, is characterized in that
(1) plastic sample spoon, fills one flat spoonful of flour quality and is about 2g;
(2) graduated disposable plastic dropper 3mL is with;
(3) 50mL tool plug glass color comparison tube;
(4) 50mL glass or plastic cylinder;
(5) test tube rack, the diameter in hole is applicable to 50mL tool plug glass color comparison tube;
(6) standard color comparison card, standard color comparison card is the serial colour atla that pale red to peony changes in gradient, the content of the calper calcium peroxide of its representative all corresponding below every bar;
(7) peroxidase chromogenic reagent;
(8) wide pH value test paper (1-14) and accurate pH test paper (6.4-8.0);
(9) reaction reagent: the reagent of three plastic bottles, every bottle amasss 500-1000mL, is respectively 1% dilute sulfuric acid, 20% dilute sodium hydroxide, 0.1% NaOH;
(10) 500mL plastic squeeze bottle.
The application of calper calcium peroxide quick detection kit in flour, its concrete steps are:
(1) get one spoonful of sample, add 1% dilute sulfuric acid 20mL, vortex mixed is even, fully reacts 10min;
(2) adding 20% sodium hydroxide solution wide pH value test paper regulates between pH to 5-6, then regulates solution ph to 7.0 with 0.1% sodium hydroxide solution.
(3) add peroxidase chromogenic reagent solution 2mL, constant volume, to 50mL, after the 5-10min that develops the color, observes color under room temperature.
(4) gained color is contrasted with standard color comparison card.
Step (3) peroxidase reagent mixed solution is made up of composite reagent, matching while using.A bottle is containing horseradish peroxidase 2000U (unit of activity); B bottle is containing phosphate buffer (pH=7.6) 200mL of the 0.2mol/L of 4-AA 0.00154mol/L; C bottle is containing 0.022mol/L phenol solution 200mL.During use, first B and C solution are fully mixed, then the enzyme in A is dissolved in mixed liquor, shakes gently, enzyme is dissolved completely, be peroxidase reagent solution, enzyme activity 10U (unit of activity)/mL.
Result judges, if sample solution nondiscolouring, does not then have admixture calper calcium peroxide, belongs to qualified flour; If solution has color to change, then for being mixed with calper calcium peroxide.Although benzoyl peroxide is also the whitening agent that flour processing is conventional, belong to strong oxidizer, under the condition of the method, under containing benzoyl peroxide 1 000mg/kg and following content condition, do not affect testing result.
The method for making of calper calcium peroxide examination criteria colorimetric card in flour:
(1) accurately take 10g and do not contain the flour of calper calcium peroxide as bare substrate, add the calper calcium peroxide standard items of precise, be mixed with the serial mark-on content of calper calcium peroxide, adding 50mL mass concentration is respectively 1% dilute sulfuric acid, vortex mixes, and fully reacts 10min.
(2) with 20% and 0.1% sodium hydroxide solution adjusted to ph to 7.0.
(3) add peroxidase composite reagent solution, in 37 DEG C of water-baths, constant temperature colour developing 20min, is cooled to room temperature, is settled to 50mL.
(4) standard solution of colour developing is gathered photo, one of graded group of photochrome is made standard color comparison card.The depth according to color carrys out quantitative and semi-quantitative.
The bare substrate mark-on concentration gradients scope prepared in method for making (1) preferably between 20mg/kg-1 000mg/kg, as 20mg/kg, 50mg/kg, 100mg/kg, 200mg/kg, 500mg/kg, 1000mg/kg.
Beneficial effect:
1) method of calper calcium peroxide in RNA isolation kit fast qualitative and half-quantitative detection flour, method is simple, quick, highly sensitive, result is distinct.
2) utilize the detection kit of calper calcium peroxide in flour of the present invention, be convenient to estimate qualitative and on-the-spot rapid screening and measure.
3) detection kit of calper calcium peroxide and detection method thereof in flour of the present invention, do not need other annex instruments, is easy to operation, practical.
Accompanying drawing explanation
Calper calcium peroxide standard color comparison card in Fig. 1 flour
Embodiment
Below the present invention is described in further details.
Embodiment 1:
The preparation of standard color comparison card
Analytical procedure: accurate weighing does not contain the wheat flour sample 10g (being accurate to 0.01g) of calper calcium peroxide whitening agent in 100mL color comparison tube, and then take high purity reagent calper calcium peroxide 0mg, 0.20mg, 0.50mg, 1.00mg, 2.00mg, 5.00mg, 10.00mg respectively and join in color comparison tube, and add 1% dilute sulfuric acid 50mL successively, vortex mixes, and at room temperature reacts 10min.Sodium hydroxide solution adjusted to ph with 20% and 0.1% is to 7.0, add 3mL enzyme reagent solution, with intermediate water constant volume to 100mL, constant temperature 10min in 37 DEG C of water-baths of band horizontal shaker, be cooled to room temperature, obtain blank sequence extraction standard solution, corresponding per kilogram flour is containing calper calcium peroxide 0mg, 20mg, 50mg, 100mg, 200mg, 500mg, 1000mg respectively.Take a picture to the solution of colour developing, gather color and be made into standard color comparison card (see Fig. 1).
Embodiment 2:
Calper calcium peroxide quick measuring reagent box
Kit is equipped with: plastic sample spoon, 2, fills one flat spoonful of flour quality and is about 2g; 3mL is with graduated disposable plastic dropper, 100; 50mL tool plug glass color comparison tube, 30; 50mL plastic cylinder, 1; Test tube rack, 1,20 holes, the diameter in hole is applicable to 50mL tool plug glass color comparison tube; Standard color comparison card, is the serial vitta changed in gradient to peony 6 containing blank and pale red, marks the content of the calper calcium peroxide of its representative below every bar by 1; Wide pH value test paper (1-14), 2 boxes; Accurate pH test paper (6.4-8.0), 2 boxes; Reaction reagent: the reagent of three kinds of plastic bottles, is denoted as 1% dilute sulfuric acid, 20% dilute sodium hydroxide, 0.1% NaOH, every bottled 500mL.500mL plastic squeeze bottle, in-built distilled water, 1.
Embodiment 3:
Analytical procedure: get certain brand dumpling flour one spoonful in 50mL color comparison tube, add 1% dilute sulfuric acid 20mL, at room temperature react 10min, after being cooled to room temperature, by 20% and 0.1% sodium hydroxide solution adjusted to ph to 7.0, add 2mL enzyme reagent solution, be settled to scale with intermediate water, shake up.Develop the color under room temperature after 5min, observe color.Sample is peach turbid solution, illustrates in this sample containing calper calcium peroxide.Through contrasting with standard color comparison card, the standard vitta color of test solution color and 100mg/kg is close, and illustrate that in this sample, calper calcium peroxide detects, content is about 100mg/kg.
Above-described embodiment is used for illustrative purposes only, and is not the restriction to patent of the present invention; It should be pointed out that for those of ordinary skill in the art, when not departing from concept of the present invention, can also make various change and modification, these all belong to protection scope of the present invention; Therefore, all equalizations done with the claims in the present invention scope change and modify, and all should belong to the coverage of the claims in the present invention.
Claims (3)
1. calper calcium peroxide quick measuring reagent box in flour, is characterized in that, the box be made up of plastic sample spoon, plastic dropper, test tube, graduated cylinder, test tube rack, standard color comparison card, peroxidase chromogenic reagent, pH test paper, reaction reagent is housed; Wherein reaction reagent comprises 1% dilute sulfuric acid, 20% dilute sodium hydroxide and 0.1% NaOH, and standard color comparison card is the serial colour atla that pale red to peony changes in gradient.
2. calper calcium peroxide quick measuring reagent box in flour according to claim 1, it is characterized in that, the pink of standard color comparison card is for be divided into 6 class from shallow to deep, and 20mg/kg is divided into the concentration gradient of the calper calcium peroxide representated by different colours correspondence mark, 50mg/kg, 100mg/kg, 200mg/kg, 500mg/kg and 1000mg/kg six class.
3. the application of calper calcium peroxide quick measuring reagent box in flour, is characterized in that, get one flat spoonful of sample, add 1% dilute sulfuric acid 20mL, and vortex mixed is even, fully reacts 10min; By sodium hydroxide solution adjust ph to 7.0; Add peroxidase chromogenic reagent 2mL, constant volume, develop the color under room temperature 5-10min, gained color contrasted with standard color comparison card, determines the content range of calper calcium peroxide in flour.
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| CN201210388832.6A CN102944550B (en) | 2012-10-15 | 2012-10-15 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
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| CN201210388832.6A CN102944550B (en) | 2012-10-15 | 2012-10-15 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424402B (en) * | 2013-07-02 | 2017-03-29 | 福建省明溪海天蓝波生物技术有限公司 | A kind of body fluid sulfydryl detection kit |
| CN103983643A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
| CN103983591B (en) * | 2014-06-03 | 2018-04-06 | 河南工业大学 | The quick determination method of calper calcium peroxide content in a kind of wheat flour |
| CN106596526B (en) * | 2015-10-20 | 2020-01-07 | 谱尼测试集团江苏有限公司 | Method for detecting content of calcium peroxide in flour |
| CN109444121A (en) * | 2018-10-16 | 2019-03-08 | 河南工业大学 | The detection kit and detection method of calper calcium peroxide in a kind of flour |
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| US4251629A (en) * | 1977-07-29 | 1981-02-17 | Wako Pure Chemical Industries, Ltd. | Determination of hydrogen peroxide |
| US4478942A (en) * | 1981-02-27 | 1984-10-23 | Fuji Photo Film Co., Ltd. | Quantitative analysis film and a method for colorimetric analysis using the same |
| CN1502990A (en) * | 2002-10-31 | 2004-06-09 | 世诺临床诊断制品株式会社 | Reagent for testing calcium in sample and test method thereof |
| CN1821754A (en) * | 2006-03-27 | 2006-08-23 | 华南农业大学 | Enzyme test peper for detecting hydrogen peroxide concentration |
| CN101034065A (en) * | 2006-03-07 | 2007-09-12 | 桑华春 | Reagent kit for quickly testing protein content of milk powder and method for making same |
| CN101393158A (en) * | 2008-10-23 | 2009-03-25 | 上海交通大学 | Biology sensing enzyme detection electrode for hydrogen peroxide and detection method thereof |
| CN101639445A (en) * | 2009-09-07 | 2010-02-03 | 福建省洪诚生物药业有限公司 | Method for detecting blood pyruvate in vitro by using chemiluminescence method |
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2012
- 2012-10-15 CN CN201210388832.6A patent/CN102944550B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4251629A (en) * | 1977-07-29 | 1981-02-17 | Wako Pure Chemical Industries, Ltd. | Determination of hydrogen peroxide |
| US4478942A (en) * | 1981-02-27 | 1984-10-23 | Fuji Photo Film Co., Ltd. | Quantitative analysis film and a method for colorimetric analysis using the same |
| CN1502990A (en) * | 2002-10-31 | 2004-06-09 | 世诺临床诊断制品株式会社 | Reagent for testing calcium in sample and test method thereof |
| CN101034065A (en) * | 2006-03-07 | 2007-09-12 | 桑华春 | Reagent kit for quickly testing protein content of milk powder and method for making same |
| CN1821754A (en) * | 2006-03-27 | 2006-08-23 | 华南农业大学 | Enzyme test peper for detecting hydrogen peroxide concentration |
| CN101393158A (en) * | 2008-10-23 | 2009-03-25 | 上海交通大学 | Biology sensing enzyme detection electrode for hydrogen peroxide and detection method thereof |
| CN101639445A (en) * | 2009-09-07 | 2010-02-03 | 福建省洪诚生物药业有限公司 | Method for detecting blood pyruvate in vitro by using chemiluminescence method |
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Address after: 201613 2nd Floor, Building 7, 99 Wenxiang East Road, Songjiang District, Shanghai Patentee after: PONY TESTING INTERNATIONAL GROUP SHANGHAI CO., LTD. Address before: 2004-233 4th Floor, Building 35, 680 Guiping Road, Xuhui District, Shanghai Patentee before: Shanghai Pony Testing Technology Co., Ltd. |
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