CN102934632B - Application of yunaconitine in pesticides - Google Patents
Application of yunaconitine in pesticides Download PDFInfo
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- CN102934632B CN102934632B CN201210471272.0A CN201210471272A CN102934632B CN 102934632 B CN102934632 B CN 102934632B CN 201210471272 A CN201210471272 A CN 201210471272A CN 102934632 B CN102934632 B CN 102934632B
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- yanaconitine
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- prevention
- yunaconitine
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- LLEMSCWAKNQHHA-IWXJPZPUSA-N yunaconitine Chemical compound O([C@H]1[C@]2(O)C[C@H]3[C@@]45[C@H]6[C@@H]([C@]([C@H]31)(C[C@@H]2OC)OC(C)=O)[C@H](OC)[C@@H]4[C@]([C@@H](C[C@@H]5OC)O)(COC)CN6CC)C(=O)C1=CC=C(OC)C=C1 LLEMSCWAKNQHHA-IWXJPZPUSA-N 0.000 title abstract 4
- UUHZKVIQJFNQRQ-UHFFFAOYSA-N yunaconitine Natural products CCN1CC2(COC)C(O)CC(OC)C34C5CC6(O)C(O)C5C(CC6OC)(OC(=O)C)C(CC23)C14 UUHZKVIQJFNQRQ-UHFFFAOYSA-N 0.000 title abstract 4
- 239000000575 pesticide Substances 0.000 title abstract 2
- 241000223194 Fusarium culmorum Species 0.000 claims abstract description 7
- 241000233679 Peronosporaceae Species 0.000 claims abstract description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000012452 mother liquor Substances 0.000 claims description 11
- 241001149504 Gaeumannomyces Species 0.000 claims description 7
- 239000003905 agrochemical Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 239000003242 anti bacterial agent Substances 0.000 abstract description 5
- 241001149475 Gaeumannomyces graminis Species 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 4
- 241000209140 Triticum Species 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 description 11
- 244000052769 pathogen Species 0.000 description 8
- 230000001717 pathogenic effect Effects 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 239000012085 test solution Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- ZMYFCFLJBGAQRS-IRXDYDNUSA-N (2R,3S)-epoxiconazole Chemical compound C1=CC(F)=CC=C1[C@@]1(CN2N=CN=C2)[C@H](C=2C(=CC=CC=2)Cl)O1 ZMYFCFLJBGAQRS-IRXDYDNUSA-N 0.000 description 3
- 239000005767 Epoxiconazole Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 241000553739 Aconitum carmichaelii var. truppelianum Species 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 241001586434 Aconitum hemsleyanum Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241001281803 Plasmopara viticola Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000520648 Pyrenophora teres Species 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 238000005162 X-ray Laue diffraction Methods 0.000 description 1
- 241001360088 Zymoseptoria tritici Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- -1 terpenoid alkaloid Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of yunaconitine in pesticides, in particular to application of yunaconitine in aspects of plant downy mildew prevention, plant netblotch germ prevention, plant wheat leaf spot disease prevention, plant gaeumannomyces graminis prevention and plant fusarium culmorum prevention. The yunaconitine can effectively restrain various phytopathogens and enlarges the selection range of antibacterial agents.
Description
Technical field
The present invention relates to a kind of new purposes of yanaconitine, be specifically related to the application of yanaconitine aspect agricultural chemicals.
Background technology
Epoxiconazole is a kind of interior absorption triazole bactericidal agent, its active component epoxiconazole suppresses the synthetic of germ ergosterol, hinder the formation of germ cell wall, can be used as spectrum antibacterial agent, be widely used on agricultural chemicals, a series of cereal crop diseases are had to good preventive and therapeutic effect as ten multiple diseases such as damping off, powdery mildew, eyeprint diseases.But because synthesis technique is complicated, domestic most dependence import.Therefore need to find a kind of wide material sources, be easy to get, and can effectively substitute the bactericide of epoxiconazole.
Yanaconitine is as a kind of terpenoid alkaloid, and Main Function has anti-inflammatory, analgesia, antipyretic and adjusting immunization.Extensively be present in the western regions of the Yunnan Province rhizome of Chinese monkshood (
aconitum hemsleyanum Pritz.var.circinatum W.T.Wang) and Dongchuan rhizome of Chinese monkshood (A
conitum geniculatunflet.et Laue.var.unguiculatum W.T.Wang) etc. in plant, wide material sources, but not yet it is carried out to sterilization, antibacterial research.
Summary of the invention
The object of the invention is the defect existing in prior art in order to solve, the antibacterial agent that provide a kind of wide material sources, is easy to get.
In order to achieve the above object, the invention provides the application of yanaconitine aspect agricultural chemicals.
Best applications anti-plant downy mildew (
plasmopara viticola), anti-plant net blotch (
pyrenophora teres), anti-plant speckled leaf blotch (
septoria tritici), anti-plant gaeumannomyce (
gaeumannomyces graminis) and anti-plant fusarium culmorum (
fusarium culmorum) aspect.
Yanaconitine, when application, first adopts DMSO to dissolve and is prepared into mother liquor.
When being applied in anti-plant downy mildew aspect, by above-mentioned mother liquor, by the concentration that 0.025% polysorbas20 is diluted to yanaconitine, be 20～200PPM(mg/L) (preferred concentration is 200PPM).
When being applied in anti-plant net blotch aspect, by above-mentioned mother liquor, by the concentration that 0.025% polysorbas20 is diluted to yanaconitine, be 200PPM.
When being applied in anti-plant speckled leaf blotch aspect, anti-plant gaeumannomyce aspect and anti-plant fusarium culmorum, by above-mentioned mother liquor, by the concentration that 0.025% polysorbas20 is diluted to yanaconitine, be 20PPM.
The present invention has the following advantages compared to existing technology: utilize yanaconitine as sterilization, antibacterial agent, can effectively suppress various plants pathogen.Yanaconitine wide material sources of the present invention, natural in synthetic, can effectively expand the scope of antibacterial agent, realize the variation of resource.
Embodiment
Below in conjunction with specific embodiment, yanaconitine of the present invention is elaborated in the application aspect agricultural chemicals.
Mother liquor configuration: get yanaconitine and dissolve with DMSO, be prepared into the mother liquor that concentration is 10mg/ml;
The configuration of test solution: during use, mother liquor is diluted to debita spissitudo with 0.025%Tween20.
Embodiment mono-
Get blade and the leaf section of plant grape in greenhouse, be placed in the porous plate that water agar cultivates (24 orifice plate), blade or leaf section are sprayed the test solution of variable concentrations in advance.After spraying, inoculation pathogen downy mildew, cultivated after 9 days, according to blade infection pathogen area calculating inhibiting rate not.Concentration is that the yanaconitine of 20PPM or 60PPM is 20% to the inhibiting rate of downy mildew, and the yanaconitine inhibiting rate that concentration is 200PPM is 50%.
Embodiment bis-
Get blade and the leaf section of barley in greenhouse, be placed in the porous plate that water agar cultivates (24 orifice plate) blade or the leaf section test solution that spray concentration is 200PPM in advance.After spraying, inoculation pathogen net blotch, cultivated after 9 days, according to blade infection pathogen area calculating inhibiting rate not.Concentration is that the yanaconitine of 20PPM is 20% to the inhibiting rate of net blotch.
Embodiment tri-
Get the mycelium of speckled leaf blotch pathogen or spore suspension (by the freezing liquid of fresh liquid medium or preservation, directly mixing nutrient broth makes), get in test solution to 96 orifice plate that 20 μ L concentration are 20PPM, then add the suspension with pathogen, 24
ounder C, 96% humidity, carry out dark culturing, after 7 days, by measuring optical density, calculate fungi inhibiting rate.Concentration is that the yanaconitine of 20PPM is 100% to the inhibiting rate of speckled leaf blotch.
Embodiment tetra-
Get the spore (suspension directly mixes nutrient broth by the freezing liquid of fresh liquid medium or preservation and makes) of gaeumannomyce, get in test solution to 96 orifice plate that 20 μ L concentration are 20PPM, then add the suspension with gaeumannomyce spore, 24
ounder C, 96% humidity, carry out dark culturing, after 7 days, by measuring optical density, calculate fungi inhibiting rate.Concentration is that the yanaconitine of 20PPM is 50% to the inhibiting rate of gaeumannomyce.
Embodiment five
The mycelium suspended liquid of extracting yellow sickle-like bacteria (directly mixing nutrient broth by the freezing liquid of fresh liquid medium or preservation makes), gets in test solution to 96 orifice plate that 20 μ L concentration are 20PPM, then adds the suspension with pathogen, 24
ounder C, 96% humidity, carry out dark culturing, after 7 days, by measuring optical density, calculate fungi inhibiting rate.Concentration is that the yanaconitine of 20PPM is 20% to the inhibiting rate of fusarium culmorum.
Claims (3)
1. the application of yanaconitine aspect agricultural chemicals, is characterized in that: the application of described yanaconitine aspect anti-plant downy mildew, anti-plant net blotch, anti-plant speckled leaf blotch, anti-plant gaeumannomyce, anti-plant fusarium culmorum.
2. application according to claim 1, it is characterized in that: when described yanaconitine is applied in anti-plant downy mildew, anti-plant net blotch, first adopt DMSO to dissolve and prepare mother liquor, before use, getting described mother liquor is 200mg/L by the concentration that 0.025% polysorbas20 is diluted to yanaconitine.
3. application according to claim 1, it is characterized in that: when described yanaconitine is applied in anti-plant speckled leaf blotch, anti-plant gaeumannomyce, anti-plant fusarium culmorum, first adopt DMSO to dissolve and prepare mother liquor, before use, getting described mother liquor is 20mg/L by the concentration that 0.025% polysorbas20 is diluted to yanaconitine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210471272.0A CN102934632B (en) | 2012-11-20 | 2012-11-20 | Application of yunaconitine in pesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210471272.0A CN102934632B (en) | 2012-11-20 | 2012-11-20 | Application of yunaconitine in pesticides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102934632A CN102934632A (en) | 2013-02-20 |
| CN102934632B true CN102934632B (en) | 2014-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210471272.0A Active CN102934632B (en) | 2012-11-20 | 2012-11-20 | Application of yunaconitine in pesticides |
Country Status (1)
| Country | Link |
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| CN (1) | CN102934632B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068045B (en) * | 2014-06-27 | 2016-06-08 | 何芳 | A kind of multi-usage pesticide composition |
| US20210100251A1 (en) * | 2017-12-19 | 2021-04-08 | Danstar Ferment Ag | Bca control of stb |
| CN115968790B (en) * | 2023-03-09 | 2023-05-16 | 西南林业大学 | Tissue culture and rapid propagation method and application of aconitum yunnanense |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543802A (en) * | 2003-11-20 | 2004-11-10 | 胡贵廷 | Biological pesticide insecticide |
| CN101502275A (en) * | 2009-03-12 | 2009-08-12 | 新疆大学 | Plant source pesticide |
| CN101731289A (en) * | 2009-12-17 | 2010-06-16 | 原春兰 | Aconitum sinomontanum water aqua pesticide and preparation method thereof |
| CN101744010A (en) * | 2008-12-19 | 2010-06-23 | 黑龙江省农业科学院植物脱毒苗木研究所 | Botanical fungicide and the preparation method thereof |
| CN102715200A (en) * | 2012-06-29 | 2012-10-10 | 陈士忠 | Pollution-free biological pesticide insecticide |
-
2012
- 2012-11-20 CN CN201210471272.0A patent/CN102934632B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543802A (en) * | 2003-11-20 | 2004-11-10 | 胡贵廷 | Biological pesticide insecticide |
| CN101744010A (en) * | 2008-12-19 | 2010-06-23 | 黑龙江省农业科学院植物脱毒苗木研究所 | Botanical fungicide and the preparation method thereof |
| CN101502275A (en) * | 2009-03-12 | 2009-08-12 | 新疆大学 | Plant source pesticide |
| CN101731289A (en) * | 2009-12-17 | 2010-06-16 | 原春兰 | Aconitum sinomontanum water aqua pesticide and preparation method thereof |
| CN102715200A (en) * | 2012-06-29 | 2012-10-10 | 陈士忠 | Pollution-free biological pesticide insecticide |
Non-Patent Citations (4)
| Title |
|---|
| 乌头的研究进展;高文韬;《北京大学学报》;20090410;第10卷(第2期);全文 * |
| 白喉乌头中新二萜生物碱;魏孝义等;《植物学报》;19961201;第38卷(第12期);全文 * |
| 白喉乌头中的二萜生物碱成分;魏孝义等;《中草药》;19950715(第07期);全文 * |
| 黔南州常见植物性土农药的种类及应用;王亚维等;《安徽农业科学》;20090210;第37卷(第5期);第2067页第2.9节 * |
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| Publication number | Publication date |
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| CN102934632A (en) | 2013-02-20 |
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Effective date of registration: 20220429 Address after: 414300 Room 303, floor 3, Linxiang Industrial Park Management Committee Office building, Ruxi community, Jiangnan Town, Linxiang City, Yueyang City, Hunan Province Patentee after: Hunan Weimo New Material Co.,Ltd. Address before: 210014, No. 1, village, Nanjing village, Xuanwu District, Zhongshan, Jiangsu Patentee before: INSTITUTE OF BOTANY,JIANGSU PROVINCE AND CHINESE ACADEMY OF SCIENCES |
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Application publication date: 20130220 Assignee: HUNAN GOFAR FINE CHEMICAL TECH. CO.,LTD. Assignor: Hunan Weimo New Material Co.,Ltd. Contract record no.: X2023980047359 Denomination of invention: The application of Dianwu alkaloid in pesticides Granted publication date: 20140409 License type: Common License Record date: 20231121 |
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