CN102925356A - Cell culture insert - Google Patents
Cell culture insert Download PDFInfo
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- CN102925356A CN102925356A CN2011102272628A CN201110227262A CN102925356A CN 102925356 A CN102925356 A CN 102925356A CN 2011102272628 A CN2011102272628 A CN 2011102272628A CN 201110227262 A CN201110227262 A CN 201110227262A CN 102925356 A CN102925356 A CN 102925356A
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Abstract
The invention relates to a cell culture insert, wherein two different types of cells can separately and independently grow into cell populations in the same container, and then the two cell populations grow to contact each other to form a cell junction area through the cell culture insert. The cell culture insert is a transparent container, and comprises a side wall (1) and a bottom wall (2), wherein the top end of the side wall (1) is distributed with a plurality of outwardly extending side wings (3), and the bottom wall (2) comprises a platform portion (4) positioned on the middle of the bottom wall (2), arc surfaces (5) positioned on both sides of the platform portion (4), and slope-like planes (6). Two types of cells are respectively inoculated onto both sides of the platform portion (4) by a researcher, a liquid level of a culture medium is lower than the platform portion (4), the two types of the cells can grow in an isolated manner, after the cells adhere to the wall to form cell populations, the culture medium is added to the liquid level of higher than the platform portion (4), the two types of the cells grow and extend toward the dome plane, when the two types of the cells contact each other, biological behavior conditions of the two types of the cells on the dome plane cell junction area can be observed through a microscope when the two types of the cells interact.
Description
Technical field
The invention belongs to biological technical field, be specifically related to be used in the bio-science research culture vessel of culturing cell, especially in same container, can make its respectively adherent growth formation cell mass by two kinds of different cells of first isolate forster, then make two cell masses continue elongation growth until be in contact with one another and form the cell culture insert of obvious cell junction region.
Background technology
At present, the culturing bottle that is used for culturing cell in the bio-science research, culture plate, cultivation pool, the bottom of the cell culture containers such as culture dish is the plane, when the researchist inoculates certain cell in culture vessel the time, because being the substratum with liquid state, the cell that inoculation enters together injects culture vessel, cell is in suspended state in substratum, so inoculation enter the cell of culture vessel will be equably along with the distribution of culture medium of liquid state in whole container scope, leave standstill the difference of the cell category that enters according to inoculation after for some time, the cell of the kind that has is with adherent growth, the cell of the kind that has is with suspension growth, the cell of adherent growth will evenly distribute and be attached at container bottom, and the cell of suspension growth will be uniformly distributed in the substratum in the container.When only needing the characteristic of a kind of cell of research, only need in the culture vessel to cultivate a kind of cell to be studied, the researchist uses platybasic type cell culture container commonly used just can reach research purpose.But when the interaction between two kinds of different types of cells of needs research, if direct inoculation enters this two kinds of cells to be studied in platybasic type cell culture container commonly used, two kinds of cells also are distributed in the culture vessel mutual mixing equably so, so that the situation of investigator only might obtain the mutual mixings growth of two kinds of different types of cells the time, and can't obtain two kinds of cells independently be grown to respectively first cell mass again continued growth until the situation of an obvious cell mass junction region that is in contact with one another and forms.That is to say that use platybasic type cell culture container commonly used can't be studied the biological behaviour situation when being in contact with one another between the two cell mass junction region place cells, has limited to RESEARCH ON CELL-BIOLOGY.
Summary of the invention
Make two kinds of different types of cells independently be grown to respectively first cell mass in order to reach, then continued growth is until an obvious cell mass junction region that is in contact with one another and forms, and be convenient to further observe the purpose of the cell behaviors situation of two kinds of cell mass intersections, the invention provides a kind of cell culture insert, can in same container, realize making two kinds of different sorts cells independently to be grown to respectively then continued growth of cell mass to the requirement that is in contact with one another in primary stage of inoculation, and can obtain obvious cell mass junction region, the biological behaviour situation when being convenient to the investigator and observing two kinds of cell mass junction region cell interactions.
The technical solution adopted for the present invention to solve the technical problems is: this cultivation pool is designed to a container-like structure that is made of sidewall and diapire.Be distributed with the flank that several outwards stretch on the top of sidewall, be used for putting the edge in six each aperture of orifice plate, so that pick and place.
Diapire is made of the platform part that is positioned at the diapire middle part and the cambered surface and the ramped shaped plane that are positioned at these platform part both sides.Being positioned at the platform part at diapire middle part for form the zone of cell intersection for two cell masses, it is designed to the plane, is directly in the cell behaviors of microscopically observation of cell junction region for the ease of the investigator.
Be provided with cambered surface between platform part and the ramped shaped plane, namely with cambered surface platform part in smoothing junction and ramped shaped plane.Connecting with cambered surface is to creep elongation growth to platform part in order to be beneficial to cell from the ramped shaped plane on platform part both sides.
The ramped shaped plane of platform part both sides extends to the sidewall bottom and is connected with sidewall to the outer lower side.The ramped shaped plane of both sides is respectively two kinds of cell attachment growths and forms the zone of cell mass.
The angular range that the ramped shaped plane becomes with sidewall is that 30 degree are to 75 degree.The design of this angular range is beneficial to the isolation growth of primary stage of inoculation cell and later stage cell mass to the elongation growth of platform part.
The cambered surface and the ramped shaped plane that are positioned at the platform part both sides in the above structure design are all symmetrical about the major axis of platform part.Tight joint or whole cell culture insert integrated molding are water-tight to guarantee between cell culture insert sidewall and the diapire.
When inoculation entered two kinds of different types of cells, a kind of cell was inoculated in a side of platform part, and another kind of cell is inoculated in the opposite side of platform part, and the liquid level of liquid nutrient medium is no more than platform part, and such two kinds of cells can be isolated growth.Cultivation pool is inserted in the six porocyte culture plates to keep aseptic, and put into cell culture incubator and cultivate.After two kinds of equal adherent growth of cell are cell mass, increase the amount of substratum, the substratum liquid level is risen to a little more than platform part, then two kinds of cell masses all can extend to the platform part growth, when two cell masses grow to when platform part is in contact with one another, can be by the immunocytochemical stain technical finesse and the biological behaviour situation when examining under a microscope the two kinds of cell interactions in its junction region place, and compare with the cell of non-junction region.
The invention has the beneficial effects as follows: can in same container, realize making two kinds of different sorts cells independently to be grown to respectively adherent cell mass in primary stage of inoculation, then continued growth is until two groups of requirements that cell is in contact with one another, and can obtain obvious cell mass junction region, the biological behaviour when being convenient to the investigator and observing two kinds of cell mass junction region place cell interactions.Be particularly suitable for the foundation of the cytobiology model of the cytobiology model of tumor cell invasion normal tissue cell and immunocyte killing tumor cell.Simple in structure, be convenient to batch production.
Description of drawings:
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is that the one-piece construction figure of first embodiment of cell culture insert is (according to Mechanical Drawing Criterion, among the figure
It is curved surface that line represents institute's sign face, does not represent any entity structure).
Fig. 2 is that first embodiment of cell culture insert is perpendicular to the vertical section structure figure of platform part 4 major axis.
Fig. 3 is the A-A ' sectional view of Fig. 2.
Fig. 4 is the vertical section structure figure that first embodiment of cell culture insert crosses platform part 4 major axis.
Fig. 5 is the B-B ' sectional view of Fig. 4.
Fig. 6 is the one-piece construction figure of second embodiment of cell culture insert.
Fig. 7 is that second embodiment of cell culture insert is perpendicular to the vertical section structure figure of platform part 4 major axis.
Fig. 8 is the C-C ' sectional view of Fig. 7.
Fig. 9 is the vertical section structure figure that second embodiment of cell culture insert crosses platform part (4) major axis.
Figure 10 is the D-D ' sectional view of Fig. 9.
Embodiment:
In embodiment illustrated in fig. 11, this cell culture insert is designed to a cylindrical container, and material is colourless high-clarity crystal polystyrene.Inside diameter of vessel 30mm, sidewall 1 height 16mm, sidewall 1 thickness 1mm can insert in the common six porocyte culture plates.Sidewall 1 top is evenly distributed with the flank 3 of 3 outside extends parallel, the long 2mm of flank, wide 2mm, thick 1mm.Vessel bottom wall 2 thickness are 1mm, and diapire 2 is made of the platform part 4 that is positioned at diapire 2 middle parts and the cambered surface 5 and the ramped shaped plane 6 that are positioned at these platform part 4 both sides.Platform part 4 is a horizontal plane, wide 3mm, and its long axis direction is connected with sidewall 1.Be provided with cambered surface 5 between platform part 4 and the ramped shaped plane 6, namely with cambered surface 5 platform part 4 in smoothing junction and ramped shaped planes 6, these cambered surface 5 radians are 0.394.The ramped shaped plane 5 of platform part 4 both sides extends to the outer lower side with sidewall (1) and is connected, and ramped shaped plane 5 is 67.4 degree with the angle of 1 one-tenth of sidewall.Whole cell culture insert integrated molding.
The using method of present embodiment is: when inoculation enters two kinds of different types of cells, a kind of cell is inoculated in a side of platform part 4, another kind of cell is inoculated in the opposite side of platform part 4, and the liquid level of liquid nutrient medium is no more than platform part 4, and such two kinds of cells can be isolated growth.Cultivation pool is inserted in the six porocyte culture plates to keep aseptic, and put into cell culture incubator and cultivate.After two kinds of equal adherent growth of cell are cell mass, increase the amount of substratum, the substratum liquid level is risen to a little more than platform part (4), then two kinds of cell masses all can extend to platform part (4) growth, when two cell masses grow in platform part (4) when being in contact with one another, can be by the immunocytochemical stain technical finesse and the biological behaviour situation when examining under a microscope the two kinds of cell interactions in its junction region place, and compare with the cell of non-junction region.
The beneficial effect of present embodiment is: can realize making two kinds of different sorts cells independently to be grown to respectively adherent cell mass in primary stage of inoculation in same container, then continued growth is until two groups of requirements that cell is in contact with one another, and can obtain obvious cell mass junction region, the biological behaviour when being convenient to the investigator and observing two kinds of cell mass junction region place cell interactions.Be particularly suitable for the foundation of the cytobiology model of the cytobiology model of tumor cell invasion normal tissue cell and immunocyte killing tumor cell.Simple in structure, be convenient to batch production.
In embodiment illustrated in fig. 62, this cell culture insert is designed to a four-prism shape container, and material is water white glass.Quadrangular shape sidewall 1 length of side 20mm, sidewall 1 height 10mm, sidewall 1 thickness 1mm can insert in the common six porocyte culture plates.There are respectively the flank 3 of 4 outside extends parallel, flank 3 long 2mm, wide 2mm, thick 1mm in the place, 4 angles on sidewall 1 top.Vessel bottom wall 2 thickness are 1mm, and diapire 2 is made of the platform part 4 that is positioned at diapire 2 middle parts and the cambered surface 5 and the ramped shaped plane 6 that are positioned at these platform part 4 both sides.Platform part 4 is a rectangle horizontal plane, the long 20mm of wide 3mm.Be provided with cambered surface 5 between platform part 4 and the ramped shaped plane 6, namely with cambered surface 5 platform part 4 in smoothing junction and ramped shaped planes 6, these cambered surface 5 radians are 0.358.The ramped shaped plane 5 of platform part 4 both sides extends to the outer lower side with sidewall 1 and is connected, and ramped shaped plane 5 is 69.5 degree with the angle of 1 one-tenth of sidewall.Tightly engage between the cell culture insert each several part.
The using method of present embodiment is identical with embodiment 1 with beneficial effect.
Claims (5)
1. cell culture insert, container-like structure by sidewall (1) and diapire (2) formation, be distributed with the flank (3) of outside stretching, extension on the top of sidewall (1), diapire (2) is by the platform part (4) that is positioned at diapire (2) middle part and be positioned at cambered surface (5) and ramped shaped plane (6) formation of this platform part (4) both sides.
2. cell culture insert according to claim 1 and 2, it is characterized in that, the platform part (4) at diapire (2) middle part is a horizontal plane, be provided with cambered surface (5) between platform part (4) and ramped shaped plane (6), namely with cambered surface (5) platform part in smoothing junction (4) and ramped shaped plane (6).
3. cell culture insert according to claim 1 is characterized in that, the ramped shaped plane (5) that is positioned at platform part (4) both sides at diapire (2) middle part extends to the outer lower side to sidewall (1) bottom and with sidewall (1) and is connected.
4. cell culture insert according to claim 1 and 2 is characterized in that, the angular range that ramped shaped plane (5) become with sidewall (1) is that 30 degree are to 75 degree.
5. cell culture insert according to claim 1 and 2 is characterized in that, this cell culture insert is made by water white plastics or glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102272628A CN102925356A (en) | 2011-08-09 | 2011-08-09 | Cell culture insert |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102272628A CN102925356A (en) | 2011-08-09 | 2011-08-09 | Cell culture insert |
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| Publication Number | Publication Date |
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| CN102925356A true CN102925356A (en) | 2013-02-13 |
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| CN2011102272628A Pending CN102925356A (en) | 2011-08-09 | 2011-08-09 | Cell culture insert |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109642200A (en) * | 2016-08-18 | 2019-04-16 | 普兰德有限两合公司 | Ware and the device for cultivating cell are inserted into cell culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2055136U (en) * | 1989-10-04 | 1990-03-28 | 蚌埠医学院 | Culture device for anaerobic bacteria |
| CN1398296A (en) * | 2000-12-07 | 2003-02-19 | 艾菲克特细胞研究所股份有限公司 | Well unit for detecting cell chemotaxis and separating chemotactic cells |
| CN1398295A (en) * | 2000-12-07 | 2003-02-19 | 艾菲克特细胞研究所股份有限公司 | Appts. for treating sample in microamount |
| EP1847592A1 (en) * | 2006-04-21 | 2007-10-24 | Samsung Electronics Co., Ltd. | Cell co-culture apparatus for researching cell interaction |
-
2011
- 2011-08-09 CN CN2011102272628A patent/CN102925356A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2055136U (en) * | 1989-10-04 | 1990-03-28 | 蚌埠医学院 | Culture device for anaerobic bacteria |
| CN1398296A (en) * | 2000-12-07 | 2003-02-19 | 艾菲克特细胞研究所股份有限公司 | Well unit for detecting cell chemotaxis and separating chemotactic cells |
| CN1398295A (en) * | 2000-12-07 | 2003-02-19 | 艾菲克特细胞研究所股份有限公司 | Appts. for treating sample in microamount |
| EP1847592A1 (en) * | 2006-04-21 | 2007-10-24 | Samsung Electronics Co., Ltd. | Cell co-culture apparatus for researching cell interaction |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109642200A (en) * | 2016-08-18 | 2019-04-16 | 普兰德有限两合公司 | Ware and the device for cultivating cell are inserted into cell culture |
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Application publication date: 20130213 |