CN102921040A - Growth induction form absorbable patch - Google Patents
Growth induction form absorbable patch Download PDFInfo
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- CN102921040A CN102921040A CN2012104449398A CN201210444939A CN102921040A CN 102921040 A CN102921040 A CN 102921040A CN 2012104449398 A CN2012104449398 A CN 2012104449398A CN 201210444939 A CN201210444939 A CN 201210444939A CN 102921040 A CN102921040 A CN 102921040A
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- sticking patch
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Abstract
The invention relates to a growth induction form absorbable patch. Polycaprolactone (PCL) is selected as a main body material, a small amount of poly lactic-co-glycolic acid (PLGA) is added to adjust the degradation rate, and a small amount of gelatin or collagen are added to promote cell growth and proliferation. The growth induction form absorbable patch has excellent mechanical strength and flexibility of the main body material (PCL), has good biocompatibility and histocompatibility in an implantation period, can well promote regeneration and remodeling of tissues, can be completely degraded and absorbed in a human body and can be widely utilized as an internal implantation support material.
Description
Technical field:
The present invention relates to degradable high polymer material and field of compound material, be specifically related to a kind of induced growth type and can absorb sticking patch.
Background technology:
At present for the demand of organ transplantation and tissue transplantation considerably beyond quantity delivered, and this gap is also widening gradually.Organizational project is a kind of new method of a kind of reconstruction and repair deficiency or damaged tissues, and this is a kind of multidisciplinary interdisciplinary science that life science engineering is combined with artificial natural materials and environmental condition.Three very important parameters are arranged in organizational project: the condition of cell, support and cell three dimensional growth on support, in life sciences, support provides a three-dimensional template framing structure for the new organization of growing into, breed and form of cell.Support Design desirable in the organizational project need to satisfy several conditions: 1. high porosity and suitable aperture, the space of support plays an important role for the propagation of cell, cell adhesion is at rack surface, absorb nutrient substance, then by the space metabolite is transported away, the diameter of cell has determined the size of minimum-value aperture, the diameter of cell is different along with the difference of cell, so the size in aperture also must strictly be controlled, if hole too minicell just can't be passed through, too maxicell just can't adhere to; 2. high specific surface area is so that cell adhesion, growth, transfer and differentiation; 3. support must degradable, and the speed that degradation rate needs to increase with tissue be complementary; 4. biocompatibility, no matter support does not still all have toxicity to cell in the degradation process again in bulk state; 5. support must have enough mechanical property support cambiums.Develop a kind of material that meets above-mentioned requirements and become people's urgent problem.
Summary of the invention:
The object of the present invention is to provide a kind of induced growth type can absorb sticking patch.
To achieve these goals, the present invention proposes following technical scheme realization:
The induced growth type can absorb sticking patch, it is characterized in that: this sticking patch is take first group of degradable high polymer material as material of main part, and adds second group of degradable high polymer material, gelatin, adopts electrostatic spinning technique to make.
Described induced growth type can absorb sticking patch thickness 60 μ m～500 μ m.
Described first group of degradable high polymer material is PCL, number-average molecular weight 10,000～400,000.
Described second group of degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, segment mol ratio L: G=90: 10～40: 60.
Described gelatin or collagen protein are selected Type A low viscosity gelatin or low viscosity collagen protein.
The induced growth type can absorb the preparation method of sticking patch, comprises the steps:
(1) preparation macromolecular solution: first group of degradable high polymer material is dissolved in the corresponding cosolvent, is mixed with the solution that concentration is 10～60wt%; Second group of degradable high polymer material and gelatin are dissolved in the corresponding cosolvent, are mixed with the solution of concentration 5～50wt%, wherein second group of degradable high polymer material accounts for 80%～99% of gross mass, and gelatin accounts for 1%～20% of gross mass;
Or first group of degradable high polymer material, second group of degradable high polymer material, gelatin be dissolved in its cosolvent, be mixed with the solution of 10～60wt%, wherein second group of degradable high polymer material accounts for 10～90% of first group of degradable high polymer material mass fraction, and gelatin accounts for the 1-20% of first group of degradable high polymer material mass fraction;
(2) electrostatic spinning: control spinning ambient temperature is 20-30 ℃, the solution of the solution of first group of degradable high polymer material that (1) is obtained, second group of degradable high polymer material and gelatin places respectively in two feed injectors of electrospinning device, the quantitative proportion that spinning syringe needle between two kinds of solution is set is 10: 9～7: 3, on spinning to the roller receiving system; Or the mixed solution of first group of degradable high polymer material obtaining in (1), second group of degradable high polymer material, gelatin placed in the feed injector of electrospinning device, spinning is to the roller receiving system; The distance of regulating between spinning head and the roller is 7～15cm, and the air velocity in the environment is controlled at 0.5～0.8m
3/ hr opens high voltage power supply and feed injector pump, regulation voltage to 10～35KV, and the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane at swing roller.
Described cosolvent is one or more in DMF, acetone, trifluoroethanol, hexafluoroisopropanol, THF, dimethyl acetylamide, the dichloromethane.
Described electrospinning device is many spinning heads electrostatic spinning machine.
Beneficial effect of the present invention: mechanical strength and compliance with material of main part PCL excellence, the degradation rate that adds a small amount of PLGA adjusting material, and add a small amount of gelatin Promote cell's growth and propagation, within the implantation phase, have good biocompatibility and histocompatibility, and can well promote tissue regeneration and reinvent; Can degradablely absorb in vivo; Can be widely used as body and be implanted into timbering material.
The specific embodiment:
Below describe preferred implementation of the present invention, but be not to limit the present invention.
Embodiment 1:
Be prepared as follows the induced growth type and can absorb sticking patch:
(1) preparation of solution: PCL (molecular weight 150000), PLGA (molecular weight 60000) and the mixing of Type A low viscosity gelatin are dissolved in the hexafluoroisopropanol, being mixed with concentration is the electrospinning solution of 25wt%, wherein PCL: PLGA: Type A low viscosity gelatin=90: 10: 1;
(2) electrostatic spinning: the mixed solution that (1) is obtained places in the feed injector of many spinning heads electrostatic spinning machine, control spinning ambient temperature is 25 ℃, the distance of regulating between spinning head and the roller is 7～15cm, and the air velocity in the environment is controlled at 0.5～0.8m
3/ hr; Open high voltage power supply and feed injector pump, regulation voltage to 10～35KV, the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane at swing roller.
Embodiment 2:
Be prepared as follows the induced growth type and can absorb sticking patch:
(1) preparation of solution: PCL (molecular weight 100000) is dissolved in the trifluoroethanol, and being mixed with PCL concentration is the electrospinning solution of 20wt%; PLGA (molecular weight 60000) mixed with Type A low viscosity gelatin be dissolved in the hexafluoroisopropanol, being mixed with concentration is the electrospinning solution of 40wt%, wherein PLGA: Type A low viscosity gelatin=95: 5;
(2) electrostatic spinning: PCL solution and PLGA, Type A low viscosity gelatin mixed solution that (1) is obtained place respectively in two feed injectors of many spinning heads electrostatic spinning machine, the quantitative proportion that PCL solution and PLGA, Type A low viscosity gelatin mixed solution spinning syringe needle are set is 8: 2, on spinning to the roller receiving system, control spinning ambient temperature is 25 ℃, the distance of regulating between spinning head and the roller is 7～15cm, and the air velocity in the environment is controlled at 0.5～0.8m
3/ hr; Open high voltage power supply and feed injector pump, regulation voltage to 10～35KV, the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane at rotating drum.
Claims (5)
1. the induced growth type can absorb sticking patch, it is characterized in that: this sticking patch is take first group of degradable high polymer material as material of main part, and adds second group of degradable high polymer material, gelatin, adopts electrostatic spinning technique to make.
2. induced growth type according to claim 1 can absorb sticking patch, it is characterized in that: described induced growth type can absorb sticking patch thickness 60 μ m～500 μ m.
3. induced growth type according to claim 1 can absorb sticking patch, it is characterized in that: described first group of degradable high polymer material is PCL, number-average molecular weight 10,000～400,000.
4. induced growth type according to claim 1 can absorb sticking patch, it is characterized in that: described second group of degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, segment mol ratio L: G=90: 10～40: 60.
5. induced growth type according to claim 1 can absorb sticking patch, it is characterized in that: described gelatin or collagen protein are selected Type A low viscosity gelatin or low viscosity collagen protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104449398A CN102921040A (en) | 2012-11-09 | 2012-11-09 | Growth induction form absorbable patch |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104449398A CN102921040A (en) | 2012-11-09 | 2012-11-09 | Growth induction form absorbable patch |
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| CN102921040A true CN102921040A (en) | 2013-02-13 |
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| CN2012104449398A Pending CN102921040A (en) | 2012-11-09 | 2012-11-09 | Growth induction form absorbable patch |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
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| CN1961974A (en) * | 2005-11-09 | 2007-05-16 | 中国科学院化学研究所 | Nano copolymer fibrous membrane material capable of being biodegraded and absorbed and preparation process and use thereof |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| CN101530353A (en) * | 2008-04-11 | 2009-09-16 | 北京天助畅运医疗技术有限公司 | Anti-adhesion hernia repair patch |
| CN101773689A (en) * | 2010-03-29 | 2010-07-14 | 苑国忠 | Surgical repairing patch |
| KR20120097948A (en) * | 2011-02-28 | 2012-09-05 | 금오공과대학교 산학협력단 | Nano/micro hybrid fiber non-woven fabric using biodegradable polymers and method for preparing the same |
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2012
- 2012-11-09 CN CN2012104449398A patent/CN102921040A/en active Pending
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| CN1961974A (en) * | 2005-11-09 | 2007-05-16 | 中国科学院化学研究所 | Nano copolymer fibrous membrane material capable of being biodegraded and absorbed and preparation process and use thereof |
| CN101530353A (en) * | 2008-04-11 | 2009-09-16 | 北京天助畅运医疗技术有限公司 | Anti-adhesion hernia repair patch |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| CN101773689A (en) * | 2010-03-29 | 2010-07-14 | 苑国忠 | Surgical repairing patch |
| KR20120097948A (en) * | 2011-02-28 | 2012-09-05 | 금오공과대학교 산학협력단 | Nano/micro hybrid fiber non-woven fabric using biodegradable polymers and method for preparing the same |
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| 韩冰等: ""聚丙交酯/乙交酯胆道支架的体外降解规律及力学特性"", 《中国组织工程研究与临床康复》, vol. 12, no. 45, 4 November 2008 (2008-11-04), pages 8942 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
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Application publication date: 20130213 |