CN102919543B - Method for preparing multifunctional ready-to-use fermented feed for animals by composite fermentation bacteria wet process - Google Patents
Method for preparing multifunctional ready-to-use fermented feed for animals by composite fermentation bacteria wet process Download PDFInfo
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- CN102919543B CN102919543B CN2012104527490A CN201210452749A CN102919543B CN 102919543 B CN102919543 B CN 102919543B CN 2012104527490 A CN2012104527490 A CN 2012104527490A CN 201210452749 A CN201210452749 A CN 201210452749A CN 102919543 B CN102919543 B CN 102919543B
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Abstract
The invention discloses a method for preparing multifunctional ready-to-use fermented feed for animals by a composite fermentation bacteria wet process, comprising the following steps of: 1, activating bacteria, and culturing in a large scale, activating frozen lactobacillus plantarum, lactobacillus acidophilus, bacillus subtilis, streptococcus faecalis,and candida utilis, and culturing in a large scale to respectively obtain 108-109cfu/mL of lactobacillus plantarum suspension liquid, 10<8>-10<9>cfu/mL of lactobacillus acidophilus suspension liquid, 10<8>-10<9>cfu/mL of bacillus subtilis suspension liquid, 10<8>-10<9>cfu/mL of streptococcus faecalis suspension liquid and 10<8>-10<9>cfu/mL of candida utilis suspension liquid; 2, preparing a composite probiotic microecological preparation for the animals; and 3, preparing multifunctional fermented feed for the animals. The multifunctional ready-to-use fermented feed for the animals, prepared by the composite fermentation bacteria wet process, is simple in process step, low in material cost, and suitable for commercial production and has a wide application range.
Description
Technical field
The present invention relates to the animal feed technology, in particular a kind of composite zymocyte wet method prepares the method for the instant feed of animal Multifunctional fermentation.
Background technology
To the improving constantly of food, livestock products safety, quality requirements, obtain the high-quality livestock products along with people, at first high-quality feed should be arranged.Therefore, researcher, enterpriser, raiser are finding approach efficient from the safe feed to the healthy aquaculture, sustainable development.In recent years; people constantly strengthen environmental protection and feed safety consciousness; requirement is preserved, processes, is transported from cultivating to whole process and sells links and implement omnidistance quality control, and " nuisance free feed ", " organic feed ", " ecological feed ", " environment-protecting feed " etc. receive much attention.Especially microbiological feed has become at present or one of research direction in the future and focus.
When in animal intestinal, normal microbial population is disorderly, the microecosystem balance is destroyed, and animal normal stool function is obstructed, and causes disease to occur.And the traditional antibiotic animal feed that contains of the harmful microorganism in enteron aisle produces drug resistance, produce a series of thorny problems in disease treatment.Simultaneously, these feeds kill and wound the body profitable strain to a certain extent, have lost the containing to pathogenic bacteria, cause endogenous infection digestive function less loss, excite various disease of digestive tracts, to animal body immunity and organ damage, bring disaster to the large ecosystem and human health.And probio is nonspecific, can illly cure the disease, anosis diseases prevention, have no adverse reaction, especially in health care, improve best results aspect efficiency of feed utilization, help to set up and maintain the advantage state of probio at enteron aisle; Murder pathogenic microorganism by producing metabolite and physiological activator; Produce multiple digestive ferment, improve efficiency of feed utilization; This provides nutrition as animal thalline; Improve the animal immune system function, promote cub fowl gut development, reduced body eutrophication and guaranteed that animal body, environment good interaction, dynamic equilibrium, circulation continuous develop in a healthy way.It is low that some animal probiotics preparations of selling on market mainly exist viable count, and poor stability starts fermentation slowly if be used in fermented feed, and efficiency is low, and viable count is low, and under the effect of animal gastric juice, easy inactivation, limited to the beneficial effect that grows of animal.Some animal feed function singlenesses of selling on market, and have contain the hormone medicines such as antibiotic, can provide the required function of the growth of animal very limited.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of composite zymocyte wet method to prepare the method for the instant feed of animal Multifunctional fermentation for the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
A kind of composite zymocyte wet method prepares the method for animal Multifunctional fermentation feed, comprises the following steps:
Step 1, actication of culture and expansion are cultivated
After being activated respectively, enlarge cultivation, the Lactobacillus plantarum bacterial classification of refrigeration, lactobacillus acidophilus bacterial classification, bacillus subtilis, enterococcus faecalis bacterial classification and candida utili bacterial classification obtain respectively 10
8-10
9Cfu/mL Lactobacillus plantarum bacteria suspension, 10
8-10
9Cfu/mL lactobacillus acidophilus bacteria suspension, 10
8-10
9Cfu/mL bacillus subtilis bacteria suspension, 10
8-10
9Cfu/mL enterococcus faecalis bacteria suspension and 10
8-10
9Cfu/mL candida utili bacteria suspension;
The preparation of step 2, composite type animal probiotics micro-ecological formulation
Under aseptic technique, take the porous-starch of 6g-10g and 6g-10g shitosan in beaker, the sterilized water that adds 100mL, the acetic acid that adds 0.5wt%-0.8 wt %, adopt 1200 rev/mins of electric mixers to stir after 20-40 minute, shitosan and porous-starch are dissolved fully, and adding concentration is 10
8-10
9Cfu/mL compound bacteria suspension 50ml, the compound bacteria suspension is: by above-mentioned bacteria suspension according to the contained viable count of Lactobacillus plantarum bacteria suspension: the contained viable count of lactobacillus acidophilus bacteria suspension: the contained viable count of bacillus subtilis bacteria suspension: the contained viable count of streptococcus fecalis bacteria suspension, the contained viable count of candida utili bacterium bacteria suspension=3: 2: 1.5: 1.5: 1 to 1: 1.5: 1.5: what the ratio of 2: 3 was mixed arrives; At room temperature adopt again supersonic generator concussion absorption 30-50 minute, through low temperature spray drying, 90 ℃～105 ℃ of inlet temperatures, 60 ℃～65 ℃ of outlet temperatures, after spray-drying, collect and obtain the composite type animal probiotics micro-ecological formulation;
The preparation of step 3, animal Multifunctional fermentation feed
The Lactobacillus plantarum bacteria suspension that step 1 is made, lactobacillus acidophilus bacteria suspension, bacillus subtilis bacteria suspension, enterococcus faecalis bacteria suspension and candida utili bacteria suspension, by 1: 1: 1: 1: 1-1: 1.5: 1.5: 1.5: 2 part by weight are mixed, get the compound micro-ecological preparation 150g-230g that 1000mL compound bacteria suspension, sterilized water 100-150mL, step 2 make, with the basic feed of 1500g microwave bacteria degermer sterilizing animal, evenly mix, adopt 900 rev/mins of electric mixers to stir after 20-40 minute, mix, through 800w, 450H
ZMicrowave bacteria degermer sterilizing 5-8min, obtain the instant feed of animal Multifunctional fermentation.
Experimental result shows, this mixed feed can obviously promote the growth of animal class, after raising young pig 45d, mass penalty has improved 13.5% than control group respectively, protein efficiency ratio, PER improves 24.5%, the incidence of disease reduces 30%-40%, this composite zymocyte wet method prepares the metabolism that the instant feed of animal Multifunctional fermentation can improve body, supplement the nutritional labeling of body, beneficial bacterium can further be bred after entering alimentary canal, produce necessary nutriment in the growth of animal process, improve the conversion ratio of feed, and the instant feed of Multifunctional fermentation prepared by a kind of wet method of the composite zymocyte for animal provided by the present invention, processing step is simple, the material therefor cost is low, be suitable for suitability for industrialized production, range of application is wide.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
It is function bacterium that the required common microorganism fungus kind of animal intestinal is take in the present invention, mainly comprise Lactobacillus plantarum (Lactobacillus plantarum, be purchased from Chinese industrial microorganism fungus kind preservation administrative center, numbering: CICC 6009), lactobacillus acidophilus (Lactobacillus acidophilus, be purchased from Chinese industrial microorganism fungus kind preservation administrative center, numbering: CICC 6075), bacillus subtilis (Bacillus subtilis, be purchased from Chinese industrial microorganism fungus kind preservation administrative center, numbering: CICC10153), enterococcus faecalis (Streptococcus faecalis, be purchased from Chinese industrial microorganism fungus kind preservation administrative center, numbering: CICC23658), candida utili (Candida utilis, be purchased from Chinese industrial microorganism fungus kind preservation administrative center, numbering: CICC1314), adopt High Density Cultivation, and adopt the moisturizing technology evenly to be mixed in animal feed, employing is fermented in feed storage and transportation, get final product the instant feed of fermented type multi-function animal of instant bagged to destination.The preparation method that the composite zymocyte wet method prepares animal Multifunctional fermentation feed is:
Step 1, actication of culture and expansion are cultivated
After being activated respectively, enlarge cultivation, the Lactobacillus plantarum bacterial classification of refrigeration, lactobacillus acidophilus bacterial classification, bacillus subtilis, enterococcus faecalis bacterial classification and candida utili bacterial classification obtain respectively 10
8-10
9Cfu/mL Lactobacillus plantarum bacteria suspension, 10
8-10
9Cfu/mL lactobacillus acidophilus bacteria suspension, 10
8-10
9Cfu/mL bacillus subtilis bacteria suspension, 10
8-10
9Cfu/mL enterococcus faecalis bacteria suspension and 10
8-10
9Cfu/mL candida utili bacteria suspension.
Lactobacillus plantarum culture medium prescription: yeast extract 5.5-7.5g, peptone 6-7.5g, whey powder 6-7g, beef extract 10-13g, glucose 10-13g, sodium chloride 3-6g, KH
2PO
42-3.5g, the 80-100ml of Tomato juice, Tween-80 0.5-0.8ml, distilled water 1000ml, pH6.9-7.1.
Lactobacillus acidophilus culture medium prescription: peptone 7-9g, yeast extract 6-8g, whey powder 7-8g, beef extract 13-15 g, glucose 13-15 g, sodium acetate 4-6 g, sodium chloride 3-5g, ammonium citrate 1.8-2.4g, Tween-80 0.8-1.2ml, MgSO
47H
2O 0.5-0.63g, MnSO
44H
2O 0.05-0.08 g, K
2HPO
41-4 g, water 1000 mL.
Bacillus subtilis culture medium prescription: glucose 3-5g, peptone 2-4g, yeast extract 3-5g, potassium dihydrogen phosphate 0.3-0.5g, calcium carbonate 0.2-0.4g, sodium chloride 4-5g, water 1000 mL.
Enterococcus faecalis culture medium prescription: peptone 6-9g, maltose 16-19g, yeast extract 5-7g, sodium glycero-phosphate 6-8g, sodium chloride 4-6g, lactose 0.7-1.2g, bromocresol purple 0.01g, Sodium azide 0.35g, TTC 0.1g, distilled water 1000ml, pH value 7.1-7.3.
Candida utili culture medium prescription: glucose 35-40 g, peptone 10-15 g, potassium dihydrogen phosphate 2-5g, MgSO
47H
2O 0.2-0.5g, sodium chloride 1-2g, distilled water 1000ml.
The preparation of step 2, composite type animal probiotics micro-ecological formulation
Under aseptic technique, take the porous-starch of 6g-10g and 6g-10g shitosan in beaker, the sterilized water that adds 100mL, the acetic acid that adds 0.5wt%-0.8 wt %, adopt 1200 rev/mins of electric mixers to stir after 20-40 minute, shitosan and porous-starch are dissolved fully, and adding concentration is 10
8-10
9cfu/mL compound bacteria suspension 50ml(by above-mentioned bacteria suspension according to the contained viable count of Lactobacillus plantarum bacteria suspension: the contained viable count of lactobacillus acidophilus bacteria suspension: the contained viable count of bacillus subtilis bacteria suspension: the contained viable count of streptococcus fecalis bacteria suspension, the contained viable count of candida utili bacterium bacteria suspension=3: 2: 1.5: 1.5: 1 to 1: 1.5: 1.5: the ratio of 2: 3 mix to the compound bacteria suspension), at room temperature adopt again supersonic generator concussion absorption 30-50 minute, through low temperature spray drying, 90 ℃～105 ℃ of inlet temperatures, 60 ℃～65 ℃ of outlet temperatures, after spray-drying, collection obtains the composite type animal probiotics micro-ecological formulation.
The preparation of step 3, animal Multifunctional fermentation feed
The Lactobacillus plantarum bacteria suspension that step 1 is made, lactobacillus acidophilus bacteria suspension, bacillus subtilis bacteria suspension, enterococcus faecalis bacteria suspension and candida utili bacteria suspension, by 1: 1: 1: 1: 1-1: 1.5: 1.5: 1.5: 2 part by weight are mixed, get the compound micro-ecological preparation 150g-230g that 1000mL compound bacteria suspension, sterilized water 100-150mL, step 2 make, with the basic feed of 1500g microwave bacteria degermer sterilizing animal, evenly mix, adopt 900 rev/mins of electric mixers to stir after 20-40 minute, mix, through 800w, 450H
ZMicrowave bacteria degermer sterilizing 5-8min, obtain the composite zymocyte wet method and prepare the instant feed of animal Multifunctional fermentation; The basic feed formula of animal: wheat bran 1000g, bean dregs 7-11g, groundnut kernel powder 5-8g, WPC matter 3-5g, soybean protein isolates 8-10g, starch 13-15g, soy meal 10-13g, sodium chloride 5-8g.
This animal Multifunctional fermentation feed 8-12 hour after preparation, can start fermentation, in storage or transportation, within 3-5 days, initial fermentation completes, and obtains the instant feed of animal Multifunctional fermentation prepared by the composite zymocyte wet method, in addition, compound micro-ecological preparation in feed is under the effect of animal gastric juice, and the wall material is partly dissolved, and after enteron aisle, adheres to animal intestinal, a large amount of propagation, the physiological function of performance composite animal probio.This composite zymocyte wet method prepares the instant feed of animal Multifunctional fermentation, the cultivation pig, ox, in the animals such as sheep, growing animal is raised 3-5 time every day, adults is raised 5-7 time, each 4kg-6kg, also can be adjusted according to actual conditions, experimental result shows, this mixed feed can obviously promote the growth of animal class, after raising young pig 45d, mass penalty has improved 13.5% than control group respectively, protein efficiency ratio, PER improves 24.5%, the incidence of disease reduces 30%-40%, this composite zymocyte wet method prepares the metabolism that the instant feed of animal Multifunctional fermentation can improve body, supplement the nutritional labeling of body, beneficial bacterium can further be bred after entering alimentary canal, produce necessary nutriment in the growth of animal process, improve the conversion ratio of feed, and the instant feed of Multifunctional fermentation prepared by a kind of wet method of the composite zymocyte for animal provided by the present invention, processing step is simple, the material therefor cost is low, be suitable for suitability for industrialized production, range of application is wide.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. a composite zymocyte wet method prepares the method for animal Multifunctional fermentation feed, it is characterized in that, comprises the following steps:
Step 1, actication of culture and expansion are cultivated
After being activated respectively, enlarge cultivation, the Lactobacillus plantarum bacterial classification of refrigeration, lactobacillus acidophilus bacterial classification, bacillus subtilis, enterococcus faecalis bacterial classification and candida utili bacterial classification obtain respectively 10
8-10
9Cfu/mL Lactobacillus plantarum bacteria suspension, 10
8-10
9Cfu/mL lactobacillus acidophilus bacteria suspension, 10
8-10
9Cfu/mL bacillus subtilis bacteria suspension, 10
8-10
9Cfu/mL enterococcus faecalis bacteria suspension and 10
8-10
9Cfu/mL candida utili bacteria suspension;
The preparation of step 2, composite type animal probiotics micro-ecological formulation
Under aseptic technique, take the porous-starch of 6g-10g and 6g-10g shitosan in beaker, the sterilized water that adds 100mL, the acetic acid that adds 0.5wt%-0.8wt%, adopt 1200 rev/mins of electric mixers to stir after 20-40 minute, shitosan and porous-starch are dissolved fully, and adding concentration is 10
8-10
9Cfu/mL compound bacteria suspension 50ml, the compound bacteria suspension is: by above-mentioned bacteria suspension according to the contained viable count of Lactobacillus plantarum bacteria suspension: the contained viable count of lactobacillus acidophilus bacteria suspension: the contained viable count of bacillus subtilis bacteria suspension: the contained viable count of streptococcus fecalis bacteria suspension: the contained viable count of candida utili bacterium bacteria suspension=3: 2: 1.5: 1.5: 1 to 1: 1.5: 1.5: the ratio of 2: 3 was mixed to get; At room temperature adopt again supersonic generator concussion absorption 30-50 minute, through low temperature spray drying, 90 ℃～105 ℃ of inlet temperatures, 60 ℃～65 ℃ of outlet temperatures, after spray-drying, collect and obtain the composite type animal probiotics micro-ecological formulation;
The preparation of step 3, animal Multifunctional fermentation feed
The Lactobacillus plantarum bacteria suspension that step 1 is made, the lactobacillus acidophilus bacteria suspension, the bacillus subtilis bacteria suspension, enterococcus faecalis bacteria suspension and candida utili bacteria suspension, by 1: 1: 1: 1: 1-1: 1.5: 1.5: 1.5: 2 part by weight are mixed, get 1000mL compound bacteria suspension, sterilized water 100-150mL, the compound micro-ecological preparation 150g-230g that step 2 makes, with the basic feed of 1500g microwave bacteria degermer sterilizing animal, evenly mix, adopt 900 rev/mins of electric mixers to stir after 20-40 minute, mix, through 800w, 450HZ microwave bacteria degermer sterilizing 5-8min, obtain the instant feed of animal Multifunctional fermentation, the basic feed formula of animal: wheat bran 1000g, bean dregs 7-11g, groundnut kernel powder 5-8g, WPC matter 3-5g, soybean protein isolates 8-10g, starch 13-15g, soy meal 10-13g, sodium chloride 5-8g.
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| CN104041706A (en) * | 2014-06-05 | 2014-09-17 | 四川农业大学 | Isoacids additive for promoting synthesis of rumen microorganism protein of ruminants |
| CN106615845A (en) * | 2017-01-11 | 2017-05-10 | 郑树彬 | Biologically fermented complete feed for pigs |
| CN110643523A (en) * | 2018-06-27 | 2020-01-03 | 无锡三智生物科技有限公司 | Formula and preparation method of fermentation microbial inoculum for fermented feed |
| CN111053148A (en) * | 2019-12-31 | 2020-04-24 | 湖南合鸣生态农业科技控股有限公司 | Micro-storage feed leaven, micro-storage feed fermented by micro-storage feed leaven and production device |
| CN111903455B (en) * | 2020-08-07 | 2022-03-25 | 江西天韵农业开发股份有限公司 | Comprehensive rice and duck planting and breeding method |
| CN113462620A (en) * | 2021-08-26 | 2021-10-01 | 福建傲农生物科技集团股份有限公司 | Preparation method and application of composite microbial agent for feed |
| CN116058432B (en) * | 2023-03-02 | 2023-06-16 | 莱芜泰禾生化有限公司 | Citric acid mycelium residue microbial feed additive and preparation method and application thereof |
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| CN101411393B (en) * | 2008-11-28 | 2011-12-14 | 李绍章 | Method for preparing active wet mixing feed fermented from liquid state to solid-state containing probiotics |
| CN102415480B (en) * | 2010-09-28 | 2014-08-27 | 张海员 | Microbial liquid premix and preparation method thereof |
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Inventor after: Tang Jie Inventor after: Lu Jing Inventor after: Zhao Huiping Inventor after: Che Zhenming Inventor after: Xing Yage Inventor after: Xiang Wenliang Inventor after: Liu Ping Inventor after: Guan Tongwei Inventor after: Zeng Liang Inventor after: Gong Li Inventor after: Zhang Lizhu Inventor before: Che Zhenming Inventor before: Zhao Huiping Inventor before: Xing Yage Inventor before: Xiang Wenliang Inventor before: Liu Ping Inventor before: Guan Tongwei Inventor before: Zeng Liang Inventor before: Gong Li Inventor before: Zhang Lizhu Inventor before: Lu Jing |
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Free format text: CORRECT: INVENTOR; FROM: CHE ZHENMING XING YAGE XIANG WENLIANG LIU PING GUAN TONGWEI CENG LIANG GONG LI ZHANG LIZHU LU JING ZHAO HUIPING TO: TANG JIE CHE ZHENMING XING YAGE XIANG WENLIANG LIU PING GUAN TONGWEI CENG LIANG GONG LI ZHANG LIZHU LU JING ZHAO HUIPING |
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