CN102911880A - Monascus and aspergillus oryzae composite fermented inoculant for improving color of Pi County bean paste and preparation method thereof - Google Patents

Abstract

The invention discloses a monascus and aspergillus oryzae composite fermented inoculant for improving the color of Pi County bean paste and a preparation method thereof. The preparation method includes the following steps: preparing first bacterial powder, inoculating 10L of a monascus strain culture solution at the middle logarithmic growth phase into a fermentation tank of 1000L for culture to obtain monascus powder with the variable count of about 109-1010CFU/g; preparing second bacterial powder, respectively inoculating 5L of an aspergillus oryzae culture solution at the middle logarithmic growth phase into the fermentation tank of 1000L for culture to obtain aspergillus oryzae powder with the variable count of about 1010-1011 CFU/g; and mixing the first bacterial powder and the second powder according to the ratio of 1: 1-1: 3, and performing vacuum packing to obtain the monascus and aspergillus oryzae composite fermentation inoculant for improving the color of the Pi County bean paste. According to the fermentation inoculant and the preparation method thereof, sensory quality is improved, flavor, unique features and nutritive values of the traditional Pi County bean paste products are maintained, and the specific health care effect of the aspergillus oryzae in the fermentation process is provided simultaneously.

Description

A kind of monascus that improves the bean paste color and luster and aspergillus oryzae complex ferment microbial inoculum and preparation method thereof

Technical field

What the present invention relates to is a kind of monascus that improves the bean paste color and luster and aspergillus oryzae complex ferment microbial inoculum and preparation method thereof.

Background technology

Bean paste is one of Sichuan three your name's lobes.Having that pungent is heavy, scarlet glossy, the capsicum piece is large, aftertaste is fragrant and sweet characteristics, is seasoning good merchantable brand commonly used in the flavor recipe of river, and the title of " Sichuan cuisine soul " is arranged.The desirable color of bean paste should be and is sorrel, and gloss is glossy, the lobe grain is neat, sticky appropriateness.But in actual production, it is immature to ferment not exclusively, individually because Exposure to Sunlight time, rainy day leak, ferment, and causes the color and luster of bean paste as one wishes not to the utmost, affects to a certain extent organoleptic quality and the marketable value of bean paste.The fast development of the bean paste industry that this problem perplexs always.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of monascus that improves the bean paste color and luster and aspergillus oryzae complex ferment microbial inoculum and preparation method thereof for the deficiencies in the prior art.

Technical scheme of the present invention is as follows:

A kind of preparation method who improves monascus and the aspergillus oryzae complex ferment microbial inoculum of bean paste color and luster comprises the following steps:

Make bacterium powder 1: the monascus strain nutrient solution 10L in logarithmic growth mid-term is linked in the 1000L fermentor tank cultivates, culture condition: 4.25% sweet potato powder, 1.83% Semen Maydis powder, 0.2% glycerine, 2% peptone, 0.2% ammonium nitrate, 0.20% ammonium chloride, 0.4% sal epsom, 0.20% potassium primary phosphate, after 30 ℃ of initial pH5.0 leavening temperatures, fermentation 96h in nutrient solution cell density can reach 1.89～2.52 * 10 ⁹CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, freezing in conjunction with vacuum drying technique through low temperature spray drying or carbonic acid gas dry ice high reactivity, colony counting method detects the bacterium number, obtains viable count and is about 10 ⁹～10 ¹⁰The monascus ruber pulvis of CFU/g;

Make bacterium powder 2: respectively the nutrient solution 5L of the aspergillus oryzae in logarithmic growth mid-term is linked in the fermentor tank of 1000L and cultivates, culture condition: 5% soybean protein isolate, 2% Semen Maydis powder, 1% peptone, 0.7% saltpetre, 0.08% sal epsom, 0.15% Sodium phosphate dibasic, after 32 ℃ of initial pH6.5 leavening temperatures, fermentation 48h, in nutrient solution, cell density maintains 1.78～2.36 * 10 ¹⁰CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, freezing in conjunction with vacuum drying technique through low temperature spray drying or carbonic acid gas dry ice high reactivity, colony counting method detects the bacterium number, obtains viable count and is about 10 ¹⁰～10 ¹¹The aspergillus oryzae pulvis of CFU/g;

With above-mentioned bacterium powder 1 and bacterium powder 2 according to 1:1～1:3(weight) mix, and namely become a kind of monascus and aspergillus oryzae complex ferment microbial inoculum that improves the bean paste color and luster through vacuum packaging.

The present invention is take monascus and aspergillus oryzae as main fermented bacterium, adopt modern biological culture technigue and low temperature spray drying technology or dry ice high reactivity dry technology to prepare a kind of complex ferment microbial inoculum that improves the bean paste color and luster, this fermenting agent not only can improve the color and luster of traditional bean paste product more than 30%, promote organoleptic quality, but also traditional bean paste product special flavour, characteristic and nutritive value have been kept, simultaneously, also increased distinctive health-care effect in the aspergillus oryzae fermenting process.

Embodiment

Below in conjunction with specific embodiment, the present invention is described in detail.

A kind ofly improve the monascus of bean paste color and luster and the preparation method of aspergillus oryzae complex ferment microbial inoculum is:

Make bacterium powder 1: with the logarithmic growth monascus in mid-term (Monascus sp., be purchased from Chinese industrial microbial strains preservation administrative center, numbering: CICC5041) strain cultured solution 10L is linked in the 1000L fermentor tank and cultivates, culture condition: 4.25% sweet potato powder, 1.83% Semen Maydis powder, 0.2% glycerine, 2% peptone, 0.2% ammonium nitrate, 0.20% ammonium chloride, 0.4% sal epsom, 0.20% potassium primary phosphate, after 30 ℃ of initial pH5.0 leavening temperatures, fermentation 96h in nutrient solution cell density can reach 1.89～2.52 * 109CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, freezing in conjunction with vacuum drying technique through low temperature spray drying or carbonic acid gas dry ice high reactivity, colony counting method detects the bacterium number, obtains viable count and is about 10 ⁹～10 ¹⁰The monascus ruber pulvis of CFU/g;

Make bacterium powder 2: respectively with the logarithmic growth aspergillus oryzae in mid-term (Aspergillus oryzae, be purchased from Chinese industrial microbial strains preservation administrative center, numbering: nutrient solution 5L CICC2120) is linked in the fermentor tank of 1000L and cultivates, culture condition: 5% soybean protein isolate, 2% Semen Maydis powder, 1% peptone, 0.7% saltpetre, 0.08% sal epsom, 0.15% Sodium phosphate dibasic, after 32 ℃ of initial pH6.5 leavening temperatures, fermentation 48h, in nutrient solution, cell density maintains 1.78～2.36 * 10 ¹⁰CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, through low temperature spray drying or dry ice high reactivity freeze drying technology, colony counting method detects the bacterium number, obtains viable count and is about 10 ¹⁰～10 ¹¹The aspergillus oryzae pulvis of CFU/g;

With above-mentioned bacterium powder 1 and bacterium powder 2 according to 1:1～1:3(weight) mix, and namely become a kind of monascus and aspergillus oryzae complex ferment microbial inoculum that improves the bean paste color and luster through vacuum packaging.

this monascus and aspergillus oryzae complex ferment microbial inoculum, add in the koji operation of producing bean paste, adding proportion is the 3wt%～6wt% of bent material, add front with 150% of complex ferment microbial inoculum quality, temperature is the warm water activation of 35～37 ℃, then stir with the song material, other techniques are identical with traditional production technique, fermentation time shortens 10%～13% than original, the look valency of bean paste product improves 25%-30%, not only can improve the look valency of bean paste product, shorten fermentation time, guarantee fermentation bean cotyledon quality homogeneous, eliminate physical environment to the impact of fermentation, and can reproduce the distinctive local flavor of traditional bean paste.Therefore can be used as a kind of complex ferment microbial inoculum that improves the bean paste color and luster, solve the defective of bean paste production product color deficiency, the look valency that obviously improves the bean paste product improves 25%-30%, by the subjective appreciation group that is formed by 20 people, be divided into best result with 9, to not using this monascus and aspergillus oryzae complex ferment microbial inoculum and using the bean cotyledon product of complex ferment microbial inoculum to carry out sensory evaluation, estimate score and be respectively 7.53 and 8.24 minutes respectively.Greatly improved the organoleptic quality of product.

Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improve and conversion all should belong to the protection domain of claims of the present invention.

Claims (2)

1. a preparation method who improves monascus and the aspergillus oryzae complex ferment microbial inoculum of bean paste color and luster, is characterized in that, comprises the following steps:

Make bacterium powder 1: the monascus strain nutrient solution 10L in logarithmic growth mid-term is linked in the 1000L fermentor tank cultivates, culture condition: 4.25% sweet potato powder, 1.83% Semen Maydis powder, 0.2% glycerine, 2% peptone, 0.2% ammonium nitrate, 0.20% ammonium chloride, 0.4% sal epsom, 0.20% potassium primary phosphate, after 30 ℃ of initial pH5.0 leavening temperatures, fermentation 96h in nutrient solution cell density can reach 1.89～2.52 * 10 ⁹CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, freezing in conjunction with vacuum drying technique through low temperature spray drying or carbonic acid gas dry ice high reactivity, colony counting method detects the bacterium number, obtains viable count and is about 10 ⁹～10 ¹⁰The monascus ruber pulvis of CFU/g;

Make bacterium powder 2: respectively the nutrient solution 5L of the aspergillus oryzae in logarithmic growth mid-term is linked in the fermentor tank of 1000L and cultivates, culture condition: 5% soybean protein isolate, 2% Semen Maydis powder, 1% peptone, 0.7% saltpetre, 0.08% sal epsom, 0.15% Sodium phosphate dibasic, after 32 ℃ of initial pH6.5 leavening temperatures, fermentation 48h, in nutrient solution, cell density maintains 1.78～2.36 * 10 ¹⁰CFU/mL; Fermented liquid filters through 100 order strainers, the porous-starch of interpolation filtrate weight 6%, sucrose and the filtrate of filtrate weight 8% weigh 1% glycerine in filtrate, freezing in conjunction with vacuum drying technique through low temperature spray drying or carbonic acid gas dry ice high reactivity, colony counting method detects the bacterium number, obtains viable count and is about 10 ¹⁰～10 ¹¹The aspergillus oryzae pulvis of CFU/g;

With above-mentioned bacterium powder 1 and bacterium powder 2 according to 1:1～1:3(weight) mix, and namely become a kind of monascus and aspergillus oryzae complex ferment microbial inoculum that improves the bean paste color and luster through vacuum packaging.

2. the monascus that improves the bean paste color and luster and the aspergillus oryzae complex ferment microbial inoculum of method according to claim 1 preparation.