CN102911258A - Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules - Google Patents
Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules Download PDFInfo
- Publication number
- CN102911258A CN102911258A CN2012102346293A CN201210234629A CN102911258A CN 102911258 A CN102911258 A CN 102911258A CN 2012102346293 A CN2012102346293 A CN 2012102346293A CN 201210234629 A CN201210234629 A CN 201210234629A CN 102911258 A CN102911258 A CN 102911258A
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- molecule
- conditioning agent
- cooh
- preparing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention relates to a method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules, belonging to the technical field of biological materials. The method comprises the following steps: (1) selecting a molecule self-assembly polypeptide, and selecting an organic micromolecule regulator; (2) thoroughly mixing the polypeptide molecule and the regulator molecule to form a mixed solution; and incubating at 37 DEG C for 7 days. The molecule regulator is introduced on the molecular level to regulate the polypeptides to form a two-dimensional self-assembly sheet structure. The scanning tunnel microtechnique on the molecular level observes that the regulator molecule can regulate the surfactant-like polypeptide assembly structure. The invention provides the interaction site between the regulator molecule and polypeptide on the molecular level, so that people can clearly understand the polypeptide assembly mechanism under the regulation of the regulator molecule.
Description
Technical field
The invention belongs to technical field of biological materials, specifically, the present invention relates to a kind of method of introducing the assembling of conditioning agent molecular regulation polypeptide and then forming two-dimentional polypeptide nano thin slice.
Background technology
As everyone knows, molecular self-assembling has become a kind of very effective approach of nanostructure for preparing, and it can be in the situation without external interference, by intermolecular each species specific interaction, the nanostructure that the inducing molecule height of formation is orderly.In biological and relevant with biology field, this technology is widely used especially.Some biomolecules or class biomolecules, peptide and protein for example, they can utilize intermolecular hydrogen bonding to interact, and the self-assemblies such as hydrophobic interaction form one dimension or zero-dimension nano structure, 1-dimention nano fiber for example, nanotube; The zero-dimension nano ball.People can be applied in every field widely with these nanostructures.For example, the fibroplastic scaffolding structure of 1-dimention nano can be applied in organizational project, and the zero-dimension nano spherical structure can be applied in the field of drug delivery.Yet so far, almost research is not reported, utilizes the nanostructure of the assembling formation sequential 2 D of polypeptide.Two-dirnentional structure is a kind of very important geometry equally, and it can possess unique surface properties, unique electrical properties, and can be used widely in all many-sides such as processing preparation of device.Between 2010-2011, professor Zuckermann of U.S. Lorentz Berkeley National Laboratory utilizes a kind of class polypeptide polymer of specially sequence, self-assembly has prepared a large amount of ultra-thin two-dimension nanocrystals, this research can help people to understand the mechanism of peptide and protein assembling by a kind of assembling process of non-natural polymeric system, and for people further design the preparation two-dimentional protein nano structure established solid basis (can be with reference to M.Reches, E.Gazit, Science 2003,300,625; E.Gazit, G.Rosenman, Journal of the American Chemical Society 2010,132,15632; R.N.Zuckermann, et al, Nature Materials 2010,9,454; R.N.Zuckermann, Journal of the American Chemical Society 2011,133,20808.).
Summary of the invention
Based on above-mentioned research as can be known, prepare a kind of two-dimentional protein nano structure very important theory significance and practical significance are arranged.The present invention has developed a kind of novel method of utilizing the terminal regulation and control of organic molecule polypeptide assembling and then forming two-dimentional polypeptide lamellar structure.
The object of the invention is to, a kind of method for preparing the polypeptide two-dimensional nanostructure is provided, carry out according to following step:
(1) select a kind of molecular self-assembling polypeptide, and select a kind of small organic molecule regulators,
(2) described peptide molecule is fully mixed with the conditioning agent molecule, form mixed solution; Under 37 ℃ of conditions, hatched 7 days.
Polypeptide for the preparation of the two-dimensional slices structure in the wherein said step (1) is a kind tensio-active agent polypeptide, and concrete structure is NH
2-KKKFAFAFAFAKKK-COOH, NH
2-KKKFFFFFFFFKKK-COOH, NH
2-AAAAAAAAKKK-COOH, NH
2-DDDFFFFFFFFDDD-COOH, NH
2-DDDAAAAAAAAKKK-COOH, NH
2-DDDFAFAFAFADDD-COOH etc., preferably class tensio-active agent peptide molecule is NH
2-KKKFAFAFAFAKKK-COOH.
Conditioning agent molecule described in the wherein said step (2) is pyrimidine, pyrazine, imidazoles, pyroles nitrogen heterocyclic molecule and derivative thereof, for example 4 ' 4-dipyridyl, vinylpyridine, and three pyridines, pyrimidine etc., preferred described tagged molecule is 4,4'-Bipyridine.
The mol ratio of wherein said peptide molecule and conditioning agent molecule is 1:1
In sum, the object of the invention is to develop a kind of novel method for preparing two-dimentional polypeptide nano structure, by adding conditioning agent in the packaging assembly of molecular level regulation and control polypeptide, impel polypeptide to form the assembling primitive of two dimension, finally form complete polypeptide two dimension assembling lamellar structure.
The present invention has following beneficial effect at least:
The present invention introduces the molecular regulation agent molecular level utilization, and the regulation and control polypeptide has formed a kind of two-dimentional self-assembly lamellar structure.The present invention utilizes technique of scan tunnel microscope to observe the regulation and control of conditioning agent molecule to class tensio-active agent polypeptide packaging assembly at molecular level.Provide conditioning agent molecule and the interactional site of polypeptide at molecular level, can make people be well understood to the mechanism of conditioning agent molecular regulation polypeptide assembling.
The present invention utilizes atomic force microscopy and transmission electron microscopy to disclose polypeptide and the assembling of conditioning agent molecule has formed the two-dimensional slices structure.Further proved the validity of regulating and control on the molecular level.
The Young's modulus of the two dimension assembling lamellar structure that the present invention utilizes quantitative nano mechanics imaging technique to study to be formed by polypeptide and conditioning agent molecule has further been surveyed the mechanical property of this two-dirnentional structure.New approaches are provided for developing a kind of novel Bio-Nano-Materials.
Description of drawings
Fig. 1 be the schematic arrangement of class tensio-active agent polypeptide and Molecular regulator and polypeptide when the conditioning agent molecule is combined by different way in conjunction with energy; Wherein a is molecule and conditioning agent molecule 4,4'-Bipyridine molecular structures synoptic diagram and the cartoon synoptic diagram of class tensio-active agent polypeptide, b, for peptide molecule when the conditioning agent molecule is combined with two kinds of different modes, simplify the cohesive energy calculation value.
Fig. 2 is the scanning tunnel microscope image that peptide molecule and conditioning agent molecule form common packaging assembly, wherein, a is peptide molecule and conditioning agent molecule 4, the scanning tunnel microscope image of the assembly that 4 '-dipyridyl forms, b are the structural representation of the assembly of peptide molecule and the formation of conditioning agent molecule;
Fig. 3 is the atomic force microscope images that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether;
Fig. 4 is the transmission electron microscope image that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether;
Fig. 5 is atomic force microscope images and the Young's modulus measurement image of peptide molecule and the conditioning agent molecule aggregate of assembling altogether rear formation; A is the height image that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether, b is the Young's modulus measurement image that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether, c is the statistics height measurements that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether, and d is the statistics Young's modulus value of peptide molecule and the conditioning agent molecule aggregate of assembling altogether rear formation.
Fig. 6 is the research that peptide molecule and conditioning agent molecule are assembled the aggregate mechanical property of rear formation altogether, a is the pattern that peptide molecule and conditioning agent molecule are assembled the aggregate of rear formation altogether, b is after the power of 2nN is sheared, peptide molecule and conditioning agent molecule are assembled the pattern of the aggregate of rear formation altogether, c and d are for before and after shearing, and peptide molecule and conditioning agent molecule are assembled the high resolution pattern of the aggregate of rear formation altogether.
Embodiment
The present invention is described in further detail below in conjunction with the drawings and specific embodiments.
1, the chemical structure of the material that uses
Peptide molecule (NH
2-KKKFAFAFAFAKKK-COOH), the chemical structure of 4,4'-Bipyridine quasi-molecule (4Bpy), as follows:
Class tensio-active agent polypeptide
4 ' 4-dipyridyl (4 ' 4-bipyridyl, 4Bpy)
2, concrete grammar
1) a kind of class tensio-active agent polypeptide that contains 8 hydrophobic groupings of design, wherein 8 amino acid are respectively FAFAFAFA, 4 phenylalanines (F) and 4 L-Ala (A), in order to regulate the water-soluble of polypeptide, respectively at 3 Methionins of two tip designs (K) of polypeptide, final polypeptide structure is NH2-KKKFAFAFAFAKKK-COOH, and the hydrophobic amino acid in this polypeptide structure will play an important role in the polypeptide assembling process, impels polypeptide to form bar Gansu Province structure.In order to form two-dimentional packaging assembly, we introduce a kind of terminal conditioning agent molecule 4,4, and ' dipyridyl molecule (4Bpy), it is terminal to be connected in each polypeptide, impels polypeptide to form the laminated structure that two dimension connects.Polypeptide structure and 4Bpy molecule are optimized, utilize molecular simulation to calculate, specifically adopt density functional theory, energy is combined in the effect that calculates respectively 4Bpy molecule and polypeptide end-COO group and-NH2 group.The theory support (as shown in Figure 1) that adjusted agent molecule is combined with polypeptide;
Fig. 1 be the schematic arrangement of class tensio-active agent polypeptide and Molecular regulator and polypeptide when the conditioning agent molecule is combined by different way in conjunction with energy; Wherein a is molecule and conditioning agent molecule 4,4'-Bipyridine molecular structures synoptic diagram and the cartoon synoptic diagram of class tensio-active agent polypeptide, b, for peptide molecule when the conditioning agent molecule is combined with two kinds of different modes, simplify the cohesive energy calculation value.
2) first peptide molecule and Pyridine Molecules are mixed in the aqueous solution, ultrasonic 1 minute, after fully mixing, under the 37oC temperature, hatched 7 days, and after this, took out 15 microlitre solution, drip to the graphite surface of new cleavage, left standstill 10 minutes, and made the mixed molecules system after graphite forms assembly and deposits from the teeth outwards, dry up with high pure nitrogen again.
Utilize commercial multiple mode scanning probe microscope (SPM, Nanoscope IIIa type, Veeco company, the U.S.), experiment condition is constant current mode under the atmosphere, and polypeptide and 4 ' 4-dipyridyl system are scanned, and obtains STM high resolution image (as shown in Figure 2);
Fig. 2 (a) is that peptide molecule and conditioning agent molecule are at the STM image of HOPG surface-assembled structure.We find that the assembly of polypeptide/4Bpy forms laminated structure, and polypeptide exists with bar Gansu Province structure, and the 4Bpy molecule is followed between Gansu Province of polypeptide, interacts with polypeptide.The conditioning agent molecule links together peptide molecule as tackiness agent at two-dimentional yardstick.The model synoptic diagram of polypeptide and 4Bpy interaction of molecules is shown in Fig. 2 (b) below.By the STM test-results that obtains, we can learn that the intermolecular hydrogen bonding that the N atom forms in the COOH group of polypeptide carboxyl terminal and the 4Bpy molecule has driven the formation of polypeptide/4Bpy assembly.In the test, the polypeptide length that we measure is 4.5nm, considers carboxyl and the amido size of end group, and the basic and theoretical length of the length that this polypeptide is measured matches.
3) will hatch peptide molecule and Pyridine Molecules mixing solutions after 7 days, take out 15 microlitres, drip to the mica surface of new cleavage, leave standstill 10 minutes, the aggregate of mixed molecules system fully is adsorbed at mica surface, dry up with high pure nitrogen again.
Utilize commercial multiple mode scanning probe microscope (SPM, Nanoscope V-type, Bruker company, the U.S.), experiment condition is to rap pattern under the atmosphere, and the aggregate system that polypeptide and 4Bpy are formed scans, and obtains afm image (as shown in Figure 3);
Fig. 3 is the two-dimensional nano thin slice pattern that peptide molecule and 4Bpy molecular self-assembling form.
4) will hatch peptide molecule and Pyridine Molecules mixing solutions after 7 days, take out 2 microlitres, drip on the copper mesh with the carbon supporting film, leave standstill 2 minutes, the aggregate of mixed molecules system fully is adsorbed on the copper mesh, then remove surplus solution.
The aggregate pattern that utilizes Philips CM20 LaB6 transmission electron microscope observation polypeptide and 4Bpy molecule to form.(as shown in Figure 4);
The transmission electron microscope image that Fig. 4 is peptide molecule and conditioning agent molecule 4Bpy assembles the aggregate of formation altogether.
5) will hatch peptide molecule and Pyridine Molecules mixing solutions after 7 days, take out 15 microlitres, drip to the mica surface of new cleavage, leave standstill 10 minutes, the aggregate of mixed molecules system fully is adsorbed at mica surface, dry up with high pure nitrogen again.
Utilize commercial multiple mode scanning probe microscope (SPM, Nanoscope V-type, Bruker company, the U.S.), experiment condition is quantitative nano mechanics imaging pattern under the atmosphere, measures the mechanical property of the two-dimensional nano thin slice of polypeptide and 4Bpy molecule formation.Specifically as illustrated in Figures 5 and 6;
Fig. 5 (a) is the height image that peptide molecule and conditioning agent molecule 4Bpy assemble the two-dimensional nano thin slice of formation altogether, its altitude distribution is 4nm, concrete the height of substrate is 0nm shown in Fig. 5 (c), and the height of the two-dimensional slices that the polypeptide assembling forms obviously is different from substrate level.Fig. 5 (b) is the Young's modulus image that peptide molecule and conditioning agent molecule 4Bpy assemble the two-dimensional nano thin slice of formation altogether.By measuring, the Young's modulus that we obtain the two-dimensional nano thin slice is 200MPa, and shown in Fig. 5 (d), this result shows that polypeptide and conditioning agent molecule form a kind of biological nano soft material.By its surperficial Young's modulus measurement is learnt, the Young's modulus on surface is 230MPa, shown in Fig. 5 (d), this does not conform to the Young's modulus of mica, this result proves, mica surface has adsorbed the assembly structure that one deck polypeptide and 4Bp form, this structure influence the measurement of Young's modulus of mica.
The present invention utilizes technique of scan tunnel microscope and atomic force microscopy and transmission electron microscopy to observe respectively, introduce the packaging assembly of conditioning agent molecular regulation amyloid polypeptide, and utilize quantitative nano mechanics imaging system, studied the mechanical property of polypeptide assembling thin slice, specifically comprised the steps:
1) preparation peptide molecule solution, ultrasonic 30 seconds;
2) in the solution of peptide molecule, add the conditioning agent molecule, it is fully mixed, hatched 7 days;
3) hatch 7 days after, with the solution of polypeptide solution and polypeptide and the blend of conditioning agent molecule, respectively get 15 microlitres and drop in respectively the high oriented graphite surface of cleavage and the mica surface of cleavage, left standstill 5 minutes;
4) with the solution of polypeptide solution and polypeptide and the blend of conditioning agent molecule, get 2 microlitres and drop on the copper mesh with the carbon supporting film, left standstill 2 minutes;
5) with high pure nitrogen the solution that remains in the surface is blown away;
6) observe with scanning tunnel microscope, observe the common packaging assembly of polypeptide and conditioning agent molecule.
7) observe the aggregate structure that aforementioned polypeptides and conditioning agent molecule form with atomic force microscope.
8) aggregate structure that forms with transmission electron microscope observation aforementioned polypeptides and conditioning agent molecule.
9) Young's modulus of the aggregate structure of usefulness quantitative nano mechanics imaging system research polypeptide and conditioning agent molecule formation, and other mechanical property of this structure.
Fig. 6 (a) is for before atomic force probe shears, the feature image of the two-dimensional slices structure that peptide molecule and 4Bpy molecule form, the A-D mark lamellar structure of formation, selection D is research object, other three thin slices thing that serves as a mark proves the research of original position.When the power of using 2nN is handled nano flake, found that after the scan operation, nano flake is sheared into two sections, shown in Fig. 6 (b).This result proves that the nano flake structure that polypeptide and 4Bpy form is a kind of soft structure, is sheared easily.Fig. 6 (c and d) is the high resolution feature image of the nano flake before and after shearing.Image shows that obviously after the shearing, lamellar structure dwindles, and proves in operating process, and this kind soft structure is out of shape extremely easily, is more prone to be pruned.
By above-mentioned five steps, our preparation has also characterized and has a kind ofly assembled altogether the two-dimensional nano lamellar structure that obtains by polypeptide and conditioning agent molecule, and this research provides a novel method for the Bio-Nano-Materials for preparing new function.
Claims (6)
1. method for preparing the polypeptide two-dimensional nanostructure is characterized in that carrying out according to following step:
(1) select a kind of molecular self-assembling polypeptide, and select a kind of small organic molecule regulators,
(2) described peptide molecule is fully mixed with the conditioning agent molecule, form mixed solution; Under 37 ℃ of conditions, hatched 7 days.
2. a kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 1 is characterized in that wherein the described molecular self-assembling polypeptide of step (1) is a kind tensio-active agent polypeptide, and concrete structure is NH
2-KKKFAFAFAFAKKK-COOH, NH
2-KKKFFFFFFFFKKK-COOH, NH
2-AAAAAAAAKKK-COOH, NH
2-DDDFFFFFFFFDDD-COOH, NH
2-DDDAAAAAAAAKKK-COOH or NH
2-DDDFAFAFAFADDD-COOH.
3. a kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 1 is characterized in that wherein conditioning agent described in the step (2) is pyrimidine, pyrazine, imidazoles, pyroles nitrogen heterocyclic molecule and derivative thereof.
4. a kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 1, the mol ratio that it is characterized in that wherein said peptide molecule and conditioning agent molecule is 1:1
A kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 2 is characterized in that wherein the described molecular self-assembling polypeptide of step (1) is NH
2-KKKFAFAFAFAKKK-COOH.
5. a kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 3 is characterized in that wherein the described conditioning agent of step (2) is 4 ' 4-dipyridyl, vinylpyridine, three pyridines, pyrimidine.
6. a kind of method for preparing the polypeptide two-dimensional nanostructure according to claim 6 is characterized in that wherein the described conditioning agent of step (2) is 4 ' 4-dipyridyl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210234629.3A CN102911258B (en) | 2012-07-06 | 2012-07-06 | Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210234629.3A CN102911258B (en) | 2012-07-06 | 2012-07-06 | Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102911258A true CN102911258A (en) | 2013-02-06 |
| CN102911258B CN102911258B (en) | 2014-07-30 |
Family
ID=47609858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210234629.3A Expired - Fee Related CN102911258B (en) | 2012-07-06 | 2012-07-06 | Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102911258B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619487A (en) * | 2018-04-19 | 2018-10-09 | 江苏大学 | Can de-assembly cationic polypeptide nano-fiber material and preparation method and purposes |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514225A (en) * | 2008-10-13 | 2009-08-26 | 西安蓝晶生物科技有限公司 | Self-polymerization polypeptide and preparation method and application thereof |
| CN101939329A (en) * | 2007-08-30 | 2011-01-05 | 滑铁卢大学 | Amino acid pairing-based self assembling peptides and methods |
| CN102115498A (en) * | 2009-12-31 | 2011-07-06 | 华中科技大学同济医学院附属协和医院 | Connexin core sequence-containing amphiphilic polypeptide and application thereof |
-
2012
- 2012-07-06 CN CN201210234629.3A patent/CN102911258B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101939329A (en) * | 2007-08-30 | 2011-01-05 | 滑铁卢大学 | Amino acid pairing-based self assembling peptides and methods |
| CN101514225A (en) * | 2008-10-13 | 2009-08-26 | 西安蓝晶生物科技有限公司 | Self-polymerization polypeptide and preparation method and application thereof |
| CN102115498A (en) * | 2009-12-31 | 2011-07-06 | 华中科技大学同济医学院附属协和医院 | Connexin core sequence-containing amphiphilic polypeptide and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 许小丁等: "多肽分子组装", 《中国科学：化学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619487A (en) * | 2018-04-19 | 2018-10-09 | 江苏大学 | Can de-assembly cationic polypeptide nano-fiber material and preparation method and purposes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102911258B (en) | 2014-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rode et al. | True atomic-resolution imaging of (1014) calcite in aqueous solution by frequency modulation atomic force microscopy | |
| Xu et al. | Recent development of PeakForce Tapping mode atomic force microscopy and its applications on nanoscience | |
| Postek et al. | Development of the metrology and imaging of cellulose nanocrystals | |
| James et al. | Interpretation of contrast in tapping mode AFM and shear force microscopy. A study of nafion | |
| Li et al. | Sequence‐Designed Peptide Nanofibers Bridged Conjugation of Graphene Quantum Dots with Graphene Oxide for High Performance Electrochemical Hydrogen Peroxide Biosensor | |
| Cassell et al. | Assembly of DNA/fullerene hybrid materials | |
| Leung et al. | Bacterial nanowires: conductive as silicon, soft as polymer | |
| Ubbink et al. | Probing bacterial interactions: integrated approaches combining atomic force microscopy, electron microscopy and biophysical techniques | |
| Kada et al. | Atomic force microscopy in bionanotechnology | |
| Li et al. | Fibroin-like peptides self-assembling on two-dimensional materials as a molecular scaffold for potential biosensing | |
| Lillehei et al. | Scanning probe microscopy | |
| Chen et al. | Applications of atomic force microscopy in materials, semiconductors, polymers, and medicine: A minireview | |
| Tian et al. | Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions | |
| Ito et al. | Polypyrrole-modified tips for functional group recognition in scanning tunneling microscopy | |
| Zhong et al. | Recent progress in the application of atomic force microscopy for supported lipid bilayers | |
| Zhu et al. | Binary nanomaterials based on nanocarbons: A case for probing carbon nanohorns' biorecognition properties | |
| Du et al. | One step electrodeposition of dendritic gold nanostructures on β-lactoglobulin-functionalized reduced graphene oxide for glucose sensing | |
| Taskin et al. | Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies | |
| Li et al. | Atomic force microscopy study of the interaction of DNA and nanoparticles | |
| Dharmasena et al. | Ultimate decoupling between surface topography and material functionality in atomic force microscopy using an inner-paddled cantilever | |
| CN102911258B (en) | Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules | |
| Xie et al. | Peptide self-assembly on mica under ethanol-containing atmospheres: effects of ethanol on epitaxial growth of peptide nanofilaments | |
| Noguchi et al. | Self-assembled GA-Repeated Peptides as a Biomolecular Scaffold for Biosensing with MoS2 Electrochemical Transistors | |
| Hiasa et al. | Mercaptohexanol assembled on gold: FM-AFM imaging in water | |
| Sun et al. | Solvent-induced assembly of microbial protein nanowires into superstructured bundles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140730 Termination date: 20160706 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |