CN102898539A - Method for subcritical water extraction of ganoderma lucidum polysaccharides - Google Patents
Method for subcritical water extraction of ganoderma lucidum polysaccharides Download PDFInfo
- Publication number
- CN102898539A CN102898539A CN 201210353836 CN201210353836A CN102898539A CN 102898539 A CN102898539 A CN 102898539A CN 201210353836 CN201210353836 CN 201210353836 CN 201210353836 A CN201210353836 A CN 201210353836A CN 102898539 A CN102898539 A CN 102898539A
- Authority
- CN
- China
- Prior art keywords
- ganoderma lucidum
- ganoderan
- extraction
- subcritical
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for subcritical water extraction of ganoderma lucidum polysaccharides. The method includes: crushing a ganoderma lucidum raw material, loading it into a subcritical extraction kettle, introducing deionized water, conducting extraction under a temperature of 100-200DEG C and a pressure of 1-20MPa for 10-80min, filtering the obtained extracted solution, carrying out ultrafiltration with an ultrafiltration membrane, collecting the concentrated solution, adding trichloroacetic acid with a concentration of 3%-10% to remove protein, performing centrifugation, adding an ethanol solution into the centrifugate to conduct precipitation, collecting the precipitates, and performing vacuum drying so as to obtain the ganoderma lucidum polysaccharides. The method for production of ganoderma lucidum polysaccharides has the advantages of high extraction efficiency, fewer solvent dosage, and high product yield, thus being easy for industrialized production.
Description
Technical field
The invention belongs to the active skull cap components separation field, relate to a kind of method of utilizing subcritical water to extract ganoderan.
Background technology
Glossy ganoderma is the treasure in motherland's pools of traditional Chinese medicine, have the reputation of " celestial grass ".At all times pharmacology and clinical study all proves, the effect that glossy ganoderma truly has and prevents and cures diseases, promotes longevity.The Compendium of Material Medica of the Shennong's Herbal of the Eastern Han Dynasty, the famous medical scholar's LI Shi-Zhen of the Ming Dynasty all has detailed very sure record to the effect of glossy ganoderma.Glossy ganoderma is formally recorded by state-promulgated pharmacopoeia as medicine, and it is again the new resource food of state approval simultaneously, has no side effect, can medicine-food two-purpose.Modern scientific research shows, the pharmaceutical component of glossy ganoderma is very abundant, wherein effective ingredient can be divided into ten large classes, comprise ganoderan, glossy ganoderma polypeptide, triterpenes, 16 seed amino acids (wherein containing seven kinds of essential amino acids), protein, N.F,USP MANNITOL, organic acid, and micro-Ge, P, Fe, Ca, Mn, Zn, etc.Glossy ganoderma has dual regulation to human body, and the kind of curing the disease relates to each systems such as cardiovascular and cerebrovascular, digestion, nerve, internal secretion, breathing, motion, and is especially very remarkable to the preventive and therapeutic effect of tumour, hepatic disease, insomnia and aging.Modern pharmacology and clinical practice have further confirmed the pharmacological action of glossy ganoderma, and confirm that ganoderan is that glossy ganoderma is strengthened the body resistance to consolidate the constitution, strengthening by means of tonics, the main component of promoting longevity.Now, glossy ganoderma is formally recorded by state-promulgated pharmacopoeia as medicine, and it is again the new resource food of state approval simultaneously, has no side effect, can medicine-food two-purpose.
Mostly the existing method of extracting ganoderan from glossy ganoderma is to adopt decocting to boil, or with in addition enzymolysis, microwave, ultrasonic assisted extraction.(application number: 200510014592.3) (application number: 200410067301.2) " a kind of preparation method of glossy ganoderma macromolecular polysaccharide " all adopts decocting to boil for " preparation method of ganoderan " and patent such as patent.For another example (application number: 201010145080.1) " a kind of preparation method of ganoderan of high yield " disclosed method is to adopt microwave-assisted to extract, and patent (application number: what 200910055092.2) " a kind of preparation method of high purity ganoderan " adopted is that enzymolysis process extracts ganoderan.Because glossy ganoderma belongs to the polyporaceae mushroom, wetting ability is relatively poor, and these method extraction efficiencies are low, and extracting cycle is long, and the product yield is lower.
Subcritical abstraction is to utilize subcritical fluids as extraction agent, in the pressurized vessel of airtight, anaerobic, low pressure, according to the similar principle that mixes of organism, by extraction material and the molecular diffusion process of extraction agent in immersion process, the composition that reaches in the solid materials is transferred in the liquid extraction agent fully.Subcritical fluid extraction is compared other separation method many advantages: the biologically active prod that keeps extract does not destroy, not oxidation, but the large large-scale industrialization production of production capacity.
Summary of the invention
The objective of the invention is to solve shortcomings and deficiencies of the prior art, a kind of method of utilizing subcritical water to extract ganoderan is provided.
To achieve these goals, the present invention is by the following technical solutions:
A kind of method of utilizing subcritical water to extract ganoderan is characterized in that: glossy ganoderma raw material pulverizing, the subcritical abstraction still of packing into, pass into deionized water, in 100 ~ 200 ℃ of temperature, pressure 1 ~ 20MPa condition, extract 10 ~ 80min, the gained extracting solution filters, use the ultra-filtration membrane ultrafiltration, collect concentrated solution, add the trichoroacetic acid(TCA) of concentration 3% ~ 10% except Deproteinization, centrifugal, centrifugate adds the ethanolic soln precipitation, the collecting precipitation thing, and vacuum-drying namely gets ganoderan.
Described ultra-filtration membrane is the hollow cellulose ultra-filtration membrane of molecular weight cut-off 5000-3000.
Advantage of the present invention is: adopt subcritical water to extract, compare with the normality water extraction, extract the solvent consumption few, energy consumption is low, and extraction time is short, and effective constituent is not lost, and whole technological operation is simple, is easy to suitability for industrialized production.
Embodiment:
Embodiment 1:
The glossy ganoderma raw material pulverizing is got 5kg, and the subcritical abstraction still of packing into passes into deionized water 25L and extracts.At 120 ℃ of temperature, pressure 1MPa condition, dynamic extraction 40min.The extracting solution cooling with the hollow cellulose film ultrafiltration of molecular weight cut-off 5000, is collected concentrated solution, add the trichoroacetic acid(TCA) of concentration 5% except Deproteinization, centrifugal, repeat 2 times, centrifugate adds 5 times of volume 95% ethanolic solns, fully stir, left standstill the collecting precipitation thing 12 hours, vacuum-drying, get ganoderan, yield 1.1%, assay 90%.
Embodiment 2:
The glossy ganoderma raw material pulverizing is got 5kg, and the subcritical abstraction still of packing into passes into deionized water 20L and extracts.At 150 ℃ of temperature, pressure 3MPa condition, dynamic extraction 30min.The extracting solution cooling with the hollow cellulose film ultrafiltration of molecular weight cut-off 3000, is collected concentrated solution, add the trichoroacetic acid(TCA) of concentration 8% except Deproteinization, centrifugal, repeat 3 times, centrifugate adds 6 times of volume 95% ethanolic solns, fully stir, left standstill the collecting precipitation thing 24 hours, vacuum-drying, get ganoderan, yield 1.0%, assay 85%.
Embodiment 3:
The glossy ganoderma raw material pulverizing is got 5kg, and the subcritical abstraction still of packing into passes into deionized water 25L and extracts.At 170 ℃ of temperature, pressure 6MPa condition, dynamic extraction 20min.The extracting solution cooling with the hollow cellulose film ultrafiltration of molecular weight cut-off 5000, is collected concentrated solution, add the trichoroacetic acid(TCA) of concentration 10% except Deproteinization, centrifugal, repeat 2 times, centrifugate adds 5 times of volume 95% ethanolic solns, fully stir, left standstill the collecting precipitation thing 12 hours, vacuum-drying, get ganoderan, yield 1.2%, assay 85%.
Claims (2)
1. a method of utilizing subcritical water to extract ganoderan is characterized in that: glossy ganoderma raw material pulverizing, the subcritical abstraction still of packing into, pass into deionized water, in 100 ~ 200 ℃ of temperature, pressure 1 ~ 20MPa condition, extract 10 ~ 80min, the gained extracting solution filters, use the ultra-filtration membrane ultrafiltration, collect concentrated solution, add the trichoroacetic acid(TCA) of concentration 3% ~ 10% except Deproteinization, centrifugal, centrifugate adds the ethanolic soln precipitation, the collecting precipitation thing, and vacuum-drying namely gets ganoderan.
2. the method for utilizing subcritical water to extract ganoderan according to claim 1 is characterized in that described ultra-filtration membrane is the hollow cellulose ultra-filtration membrane of molecular weight cut-off 5000-3000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210353836 CN102898539A (en) | 2012-09-21 | 2012-09-21 | Method for subcritical water extraction of ganoderma lucidum polysaccharides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210353836 CN102898539A (en) | 2012-09-21 | 2012-09-21 | Method for subcritical water extraction of ganoderma lucidum polysaccharides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102898539A true CN102898539A (en) | 2013-01-30 |
Family
ID=47571053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210353836 Pending CN102898539A (en) | 2012-09-21 | 2012-09-21 | Method for subcritical water extraction of ganoderma lucidum polysaccharides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102898539A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103130911A (en) * | 2013-03-22 | 2013-06-05 | 中国林业科学研究院林产化学工业研究所 | Method for extracting pleurotus eryngii polysaccharide from pleurotus eryngii leftover by using subcritical water |
| CN103880972A (en) * | 2014-03-12 | 2014-06-25 | 江苏大学 | Method of synchronously extracting polysaccharides and proteins from subcritical water |
| CN104231108A (en) * | 2014-10-15 | 2014-12-24 | 中国林业科学研究院林产化学工业研究所 | Production method for extracting tremella polysaccharide by subcritical water extraction-hot water digestion two-step method |
| CN104961839A (en) * | 2015-06-12 | 2015-10-07 | 安徽华润金蟾药业股份有限公司 | Preparation method of specific pachyman formula granule |
| CN104448027B (en) * | 2014-12-31 | 2016-08-24 | 威海人生集团股份有限公司 | Fucoidan and the method for fucoxanthine is extracted from Thallus Laminariae (Thallus Eckloniae) |
| CN106478834A (en) * | 2016-11-14 | 2017-03-08 | 江苏江大源生态生物科技股份有限公司 | A kind of method of Subcritical water chromotagraphy grifolan |
| CN108324729A (en) * | 2018-02-01 | 2018-07-27 | 江苏大学 | A kind of arrowhead polysaccharide composition and preparation method thereof of enhancing immune function |
| CN108434171A (en) * | 2018-02-01 | 2018-08-24 | 江苏大学 | A kind of arrowhead polysaccharide composition and preparation method thereof preventing chemical damage |
| CN111777691A (en) * | 2020-08-04 | 2020-10-16 | 河南白云牧港生物科技有限公司 | Extraction method of magnolia flower polysaccharide |
| JP2021038186A (en) * | 2019-09-05 | 2021-03-11 | 日本メナード化粧品株式会社 | Immunostimulant |
| JP2021038169A (en) * | 2019-09-03 | 2021-03-11 | 日本メナード化粧品株式会社 | Nutrient transport promoter |
-
2012
- 2012-09-21 CN CN 201210353836 patent/CN102898539A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103130911A (en) * | 2013-03-22 | 2013-06-05 | 中国林业科学研究院林产化学工业研究所 | Method for extracting pleurotus eryngii polysaccharide from pleurotus eryngii leftover by using subcritical water |
| CN103880972A (en) * | 2014-03-12 | 2014-06-25 | 江苏大学 | Method of synchronously extracting polysaccharides and proteins from subcritical water |
| CN104231108A (en) * | 2014-10-15 | 2014-12-24 | 中国林业科学研究院林产化学工业研究所 | Production method for extracting tremella polysaccharide by subcritical water extraction-hot water digestion two-step method |
| CN104448027B (en) * | 2014-12-31 | 2016-08-24 | 威海人生集团股份有限公司 | Fucoidan and the method for fucoxanthine is extracted from Thallus Laminariae (Thallus Eckloniae) |
| CN104961839B (en) * | 2015-06-12 | 2017-10-17 | 安徽华润金蟾药业股份有限公司 | The preparation method of one species specificity pachymaran granule |
| CN104961839A (en) * | 2015-06-12 | 2015-10-07 | 安徽华润金蟾药业股份有限公司 | Preparation method of specific pachyman formula granule |
| CN106478834A (en) * | 2016-11-14 | 2017-03-08 | 江苏江大源生态生物科技股份有限公司 | A kind of method of Subcritical water chromotagraphy grifolan |
| CN108324729A (en) * | 2018-02-01 | 2018-07-27 | 江苏大学 | A kind of arrowhead polysaccharide composition and preparation method thereof of enhancing immune function |
| CN108434171A (en) * | 2018-02-01 | 2018-08-24 | 江苏大学 | A kind of arrowhead polysaccharide composition and preparation method thereof preventing chemical damage |
| JP2021038169A (en) * | 2019-09-03 | 2021-03-11 | 日本メナード化粧品株式会社 | Nutrient transport promoter |
| JP7372656B2 (en) | 2019-09-03 | 2023-11-01 | 日本メナード化粧品株式会社 | Nutrient transport enhancer |
| JP2021038186A (en) * | 2019-09-05 | 2021-03-11 | 日本メナード化粧品株式会社 | Immunostimulant |
| JP7372659B2 (en) | 2019-09-05 | 2023-11-01 | 日本メナード化粧品株式会社 | immunostimulant |
| CN111777691A (en) * | 2020-08-04 | 2020-10-16 | 河南白云牧港生物科技有限公司 | Extraction method of magnolia flower polysaccharide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102898539A (en) | Method for subcritical water extraction of ganoderma lucidum polysaccharides | |
| CN100420665C (en) | Method for extracting 'Danfen' phenolic acid-A | |
| CN102048885B (en) | Extraction process for lychee seed saponin | |
| CN101585885A (en) | Method for preparing polygonatum odoratum polysaccharide | |
| CN102127140A (en) | Method for combinedly extracting Morroniside, dogwood polysaccharide and ursolic acid from dogwood | |
| CN110272932A (en) | A kind of preparation method of lucid ganoderma spore powder polysaccharide peptide | |
| CN102850419A (en) | Method for extracting cordycepin and polysaccharide from cordyceps militaris | |
| CN102154428B (en) | Method for preparing ginsenoside Rh2 | |
| CN102030834A (en) | Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide | |
| CN111228317A (en) | Preparation method and application of inonotus obliquus extract | |
| CN106490608A (en) | A kind of Herba Dendrobii enhancing immunity oral liquid, electuary, capsule, tablet | |
| CN102827299B (en) | Efficient extraction and purification method for kumquat polysaccharide | |
| CN103191196A (en) | Integrated ultrasonic cyclic extracting and separating technology for cistanche functional ingredient | |
| CN103304576A (en) | Method for extracting artemisinin through enzymatic hydrolysis | |
| CN104292352A (en) | Method for extracting and separating eucommia ulmoides flavor powder, eucommia ulmoides oliv polysaccharides and eucommea rubber through coproduction | |
| CN101701041B (en) | Technology for extracting ganoderma active polysaccharide at low-temperature | |
| CN106084087A (en) | A kind of preparation method of Fructus Trichosanthis polysaccharide | |
| CN101921344A (en) | Method for extracting polysaccharides from Lilium Brownii | |
| CN108323663A (en) | A kind of Radix Et Caulis Acanthopanacis Senticosi polysaccharide health beverages and the method that Radix Et Caulis Acanthopanacis Senticosi polysaccharide is extracted in joining from slender acanthopanax | |
| CN107286264A (en) | The deep working method of Chinese date nutrient material separation | |
| CN102940688A (en) | Extraction method for polysaccharides and total flavonoids in fig leaves | |
| CN102091107B (en) | Method for extracting total triterpene of centella asiatica by enzymic process | |
| CN104189087B (en) | A kind of method extracting total flavonoid functional materials from Folium Mori | |
| CN103961388B (en) | A kind of preparation method of radix cynanchi bungei total saponin | |
| CN103550264A (en) | Method for extracting Ganoderma lucidum essences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130130 |