CN102895422B - Application of tetrastlgma hemsleyanum in preparing drug for treating livestock viral infectious diseases - Google Patents
Application of tetrastlgma hemsleyanum in preparing drug for treating livestock viral infectious diseases Download PDFInfo
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Abstract
The invention discloses an application of tetrastlgma hemsleyanum in preparing a drug for treating livestock viral infectious diseases. According to the application, tetrastlgma hemsleyanum is used for treating livestock viral infectious diseases, particularly Newcastle disease, infectious bursal disease virus, avian infectious broncheitis, porcine reproductive and respiratory syndrome and porcine circovirus mixed infection; and the drug has a high curative effect and small side effects, and a new choice is provided for clinically treating livestock viral infectious diseases.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to the application in the medicine of preparation treatment livestock and poultry virus sexually transmitted disease of Radix Tetrastigmatis Hemsleyani and extract thereof.
Background technology
Along with socioeconomic development, people are more and more higher to the prescription of animal products, and poultry infectious disease is very large to aquaculture harm.For viral infectious, antibiotic, sulfa drugs are without therapeutical effect, although the minority medicines such as amantadine, ribavirin, acyclovir have certain curative effect, antiviral spectrum is narrow, toxic and side effects is large, price is high and easily produce drug resistance strain.Therefore, seek novel antiviral medicine extremely urgent.
Radix Tetrastigmatis Hemsleyani is again Radix Apioris Fortunei (Radix Lespedezae Buergeri), is the tuber of vitaceae tetratigma hemsleyanum Tetrastigma hemsleyanum Diels et Gilg, the green herbaceous species of perennation.Originate in the ground such as Zhejiang, Jiangxi, Hunan, Hubei, Fujian, Guangdong, Guangxi and Yunnan, there is the effect of heat-clearing and toxic substances removing, expelling wind and activating blood circulation.Be usually used in the cards such as hyperpyrexia convulsion, pneumonia, cough with asthma, hepatitis, nephritis, rheumatic arthralgia, traumatic injury, carbuncle furuncle furuncle, be also improved the effect of Immune Function.
So far, there is not yet the report of Radix Tetrastigmatis Hemsleyani control livestock and poultry virus sexually transmitted disease.
Summary of the invention
Technical problem to be solved by this invention is to provide the application of Radix Tetrastigmatis Hemsleyani in the medicine of preparation treatment livestock and poultry virus sexually transmitted disease.
Technical problem of the present invention is implemented by the following technical programs:
Radix Tetrastigmatis Hemsleyani is applied in the medicine of preparation treatment livestock and poultry virus sexually transmitted disease, and described livestock and poultry virus sexually transmitted disease can be newcastle disease, chicken bursal disease, infectious bronchitis of chicken, pig blue-ear disease and porcine circovirus mixed infection.Aforementioned Radix Tetrastigmatis Hemsleyani not only refers to Radix Tetrastigmatis Hemsleyani raw medicinal herbs itself, also comprise Radix Tetrastigmatis Hemsleyani processed product, Radix Tetrastigmatis Hemsleyani extract, the preparation that the preparation that the Radix Tetrastigmatis Hemsleyani of take is active component or the Radix Tetrastigmatis Hemsleyani of take are one of active component, is preferably Radix Tetrastigmatis Hemsleyani extract or take the preparation that Radix Tetrastigmatis Hemsleyani is active component.
Radix Tetrastigmatis Hemsleyani extract of the present invention is Radix Tetrastigmatis Hemsleyani water or extractive with organic solvent, can adopt the routine techniquess such as carbon dioxide supercritical extraction method, percolation, decoction, reflux to extract the extract obtaining.Described organic solvent refers to the solvent of the opposed polarities such as ethanol, methanol, and preferably adopting mass percent concentration is 75%~85% ethanol or methanol.
Water extract is preferably prepared as follows and obtains:
Get Radix Tetrastigmatis Hemsleyani, after soaking, decoct 1 ~ 4 time by 5~20 times of medical material weight, each 1 ~ 3h, merges decoction liquor, and dehydration is concentrated into thick paste shape dries and obtain Radix Tetrastigmatis Hemsleyani extract.
Extractive with organic solvent is preferably prepared as follows and obtains:
Get Radix Tetrastigmatis Hemsleyani, pulverize, by 75%~85% ethanol of 5~20 times of weight of medical material weight or methanol (mass percent concentration), soak after 1 ~ 4 hour, percolation, reclaims ethanol or methanol, is concentrated into thick paste shape, and low-temperature reduced-pressure is dried, and obtains extract.
Radix Tetrastigmatis Hemsleyani raw medicinal herbs or its extract can directly be pulverized and make powder, or add pharmaceutically acceptable adjuvant to adopt conventional method to make oral formulations or injection.Described oral formulations is preferably mixture, tablet or oral liquid.
Medium domestic animal (as pig, sheep etc.) is 5~12g each serving amount to raw medicinal herbs with Radix Tetrastigmatis Hemsleyani, and day takes twice, and poultry consumption is 1/10~1/5 of medium domestic animal.
Beneficial effect of the present invention: inventor is with livestock and poultry virus sexually transmitted diseases such as Radix Tetrastigmatis Hemsleyani or its extract for treating newcastle disease, chicken bursal disease, infectious bronchitis of chicken, porcine pseudorabies, pig blue-ear disease and porcine circovirus mixed infections, and curative effect is really stable.The invention provides Radix Tetrastigmatis Hemsleyani for the preparation of the application in the medicine for the treatment of livestock and poultry virus sexually transmitted disease, for clinical treatment livestock and poultry virus sexually transmitted disease provides a kind of new selection.
The specific embodiment
By the specific embodiment of example forms, foregoing of the present invention is described in further detail again below.But this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example.All technology realizing based on foregoing of the present invention all belong to scope of the present invention.
With regard to clinical effectiveness of the present invention, further illustrate curative effect of the present invention below:
One, Radix Tetrastigmatis Hemsleyani egg inoculation anti-new castle disease virus and pseudorabies virus test
1, medicine:
(1) Radix Tetrastigmatis Hemsleyani sample 1: according to embodiment 1 method, prepare, with normal saline, be made into every mL medicinal liquid containing the concentration of 1g crude drug.
(2) Radix Tetrastigmatis Hemsleyani sample 2: according to embodiment 2 methods, prepare, with normal saline, be made into every mL medicinal liquid containing the concentration of 1g crude drug.
(3) Radix Tetrastigmatis Hemsleyani sample 3: according to embodiment 10 methods, prepare.
(4) Western medicine contrast medicine: 50mg/mL ribavirin solution.
2, newcastle disease strain is provided by Jiangsu Animal Husbandry & Veterinary College prevention veterinary teaching and research room, the HA of the chick embryo allantoic liquid 9log that tires
2, after measured, minimum lethal dose is ten thousand times of dilutions of 1:3000.
3, pseudorabies strain holds A strain AV25 pseudorabies strain, by Chinese veterinary supervise provide, minimum lethal dose is that 1:50 doubly dilutes.
4, SPF test Embryo Gallus domesticus is tested chicken house purchased from Shandong.
5, test grouping SPF Embryo Gallus domesticus is 144, the situation of specifically dividing into groups in Table 1, table 2.
6, test method
(1) Drug toxicity trails is used respectively normal saline dilution by Radix Tetrastigmatis Hemsleyani sample 1, Radix Tetrastigmatis Hemsleyani sample 2 and Radix Tetrastigmatis Hemsleyani sample 3, the medicinal liquid of stock solution and variable concentrations is inoculated in respectively in the allantoic cavity of 3 instar chicken embryos on the 10th, every 0.2mL, put 37.5 ℃ of incubation 72h, observe survival condition every day, the Embryo Gallus domesticus all the highest liquor strengths of survival be formally to test initial concentration, establishes normal saline matched group simultaneously, medicine to Embryo Gallus domesticus toxicity experimental result in Table 3.
Table 1 newcastle disease experimental group situation
Numbering | Group | Quantity (piece) | Process |
1 | Test I group | 12 | Radix Tetrastigmatis Hemsleyani sample 10.2mL, newcastle disease virus allantoic fluid 0.1mL |
2 | Test II group | 12 | Radix Tetrastigmatis Hemsleyani sample 20.2mL, newcastle disease virus allantoic fluid 0.1mL |
3 | Test III group | 12 | Radix Tetrastigmatis Hemsleyani sample 30.2mL, newcastle disease virus allantoic fluid 0.1mL |
4 | Test IV group | 12 | Ribavirin injection 0.2mL, newcastle disease virus allantoic fluid 0.1mL |
5 | Positive controls | 12 | Virus inoculation allantoic fluid, by tested number to normal saline |
6 | Blank group | 12 | Do not inoculate, not administration |
Table 2 pseudorabies experimental group situation
Numbering | Group | Quantity (piece) | Process |
1 | Test I group | 12 | Radix Tetrastigmatis Hemsleyani sample 10.2mL, Pseudorabies virus allantoic fluid 0.1mL |
2 | Test II group | 12 | Radix Tetrastigmatis Hemsleyani sample 20.2mL, pseudorabies epidemic disease poison allantoic fluid 0.1mL |
3 | Test III group | 12 | Radix Tetrastigmatis Hemsleyani sample 30.2mL, pseudorabies epidemic disease poison allantoic fluid 0.1mL |
4 | Test IV group | 12 | Ribavirin injection 0.2mL, Pseudorabies virus allantoic fluid 0.1mL |
5 | Positive controls | 12 | Virus inoculation allantoic fluid, by tested number to normal saline |
6 | Blank group | 12 | Do not inoculate, not administration |
Table 3 Chinese medicine is to Embryo Gallus domesticus toxicity experimental result (n=4)
* denominator is inoculation embryo number, and molecule is survival embryo number.
(2) concrete grammar:
1. newcastle experimental group
Test I organizes each chick embryo allantoic cavity and inoculates Radix Tetrastigmatis Hemsleyani sample 1 stock solution 0.2mL, Avian pneumo-encephalitis virus diluent 0.1mL simultaneously.
Each chick embryo allantoic cavity of test II group is inoculated Radix Tetrastigmatis Hemsleyani sample 2 stock solution 0.2mL, Avian pneumo-encephalitis virus diluent 0.1mL simultaneously.
Test III organizes each chick embryo allantoic cavity and inoculates Radix Tetrastigmatis Hemsleyani sample 3 stock solution 0.2mL, Avian pneumo-encephalitis virus diluent 0.1mL simultaneously.
Test IV organizes the ribavirin 0.2mL that each chick embryo allantoic cavity is inoculated 50mg/mL simultaneously, Avian pneumo-encephalitis virus diluent 0.1mL.
Each chick embryo allantoic cavity of positive controls is inoculated normal saline 0.2mL simultaneously, Avian pneumo-encephalitis virus diluent 0.1mL.
Blank group is virus inoculation not, not administration.
2. pseudorabies experimental group
Test I organizes each chick embryo allantoic cavity and inoculates Radix Tetrastigmatis Hemsleyani sample 1 stock solution 0.2mL, Pseudorabies virus diluent 0.1mL simultaneously.
Each chick embryo allantoic cavity of test II group is inoculated Radix Tetrastigmatis Hemsleyani sample 2 stock solution 0.2mL, Pseudorabies virus diluent 0.1mL simultaneously.
Test III organizes each chick embryo allantoic cavity and inoculates Radix Tetrastigmatis Hemsleyani sample 3 stock solution 0.2mL, Pseudorabies virus diluent 0.1mL simultaneously.
Test IV organizes the ribavirin 0.2mL that each chick embryo allantoic cavity is inoculated 50mg/mL simultaneously, Pseudorabies virus diluent 0.1mL.
Each chick embryo allantoic cavity of positive controls is inoculated normal saline 0.2mL simultaneously, Pseudorabies virus diluent 0.1mL.
Blank group is virus inoculation not, not administration.
Above-mentioned Embryo Gallus domesticus continues hatching in 37.5 ℃ of calorstats, and shine egg 2~3 every day, discards dead germ in 24h, collects the allantoic fluid of 24~72h dead germ and 72h embryo alive, and cryopreservation is standby.With blood clotting (HA) experiment, detect the virus titer of chick embryo allantoic liquid.
(3) impact of Radix Tetrastigmatis Hemsleyani on newcastle disease virus and pseudorabies virus propagation, the results are shown in Table 4, table 5.
The inhibitory action measurement result of table 4 Radix Tetrastigmatis Hemsleyani to newcastle disease virus propagation
Grouping | The dead embryo number of 24~72h | 72h embryo number alive | HA tires |
Radix Tetrastigmatis Hemsleyani sample 1 stock solution | 2** | 10** | 0** |
Radix Tetrastigmatis Hemsleyani sample 2 stock solutions | 1** | 11** | 0** |
Radix Tetrastigmatis Hemsleyani sample 3 stock solutions | 6* | 6* | 0** |
Ribavirin group | 10* | 2* | 5log 2* |
Positive controls | 12 | 0 | 9log 2 |
Blank group | 0 | 12 | 0 |
With positive controls comparison: * P<0.05, * * P<0.01
The inhibitory action measurement result of table 5 Radix Tetrastigmatis Hemsleyani to Pseudorabies virus propagation
Grouping | The dead embryo number of 24~72h | 72h embryo number alive | HA tires |
Radix Tetrastigmatis Hemsleyani sample 1 stock solution | 3** | 9** | 0** |
Radix Tetrastigmatis Hemsleyani sample 2 stock solutions | 2** | 10** | 0** |
Radix Tetrastigmatis Hemsleyani sample 3 stock solutions | 7* | 5* | 0** |
Ribavirin group | 9* | 3* | 4log 2* |
Positive controls | 12 | 0 | 6log 2 |
Blank group | 0 | 12 | 0 |
With positive controls comparison: * P<0.05, * * P<0.01
Two, the therapeutic test of the present invention to Chickens newcastle
1, experimental animal: 150 of 35 age in days Ai Wei mattress broiler for this test, be divided at random 10 groups, every group 15, be Radix Tetrastigmatis Hemsleyani sample 1 (by embodiment 3 method preparations) prevention group and treatment group, Radix Tetrastigmatis Hemsleyani sample 2 (pressing embodiment 4 method preparations) prevention group and treatment group, Radix Tetrastigmatis Hemsleyani sample 3 (pressing embodiment 10 method preparations) prevention group and treatment group, Western medicine prevention group and treatment group, infect matched group, blank group.
2, newcastle disease strain is provided by Jiangsu animal and veterinary teaching and research room, and the HA of chick embryo allantoic liquid tires as 9log
2.
3, test method:
(1) first measure allantoic fluid minimum lethal dose, the minimum lethal dose of allantoic fluid is 100 times of dilutions after measured, the allantoic fluid 0.2mL after every chicken collunarium and eye dripping dilution.
(2) the not medication of chicken of operating space matched group, does not inoculate allantoic fluid, all the other chickens of 9 groups at one time in allantoic fluid 0.2mL after the dilution of every collunarium eye dripping.
(3) administrated method and curative effect Radix Tetrastigmatis Hemsleyani sample 1 prevention group, in inoculation allantoic fluid, start to use Radix Tetrastigmatis Hemsleyani sample 1 oral, every each 1mL of chicken, and medication every day 2 times, is used in conjunction 4d.(the occurring condition of illness) medication after inoculation allantoic fluid 3d of Radix Tetrastigmatis Hemsleyani sample 1 treatment group, every each 1mL of chicken, medication every day 2 times, is used in conjunction 4d.Radix Tetrastigmatis Hemsleyani sample 2 prevention groups are in inoculation allantoic fluid, oral with Radix Tetrastigmatis Hemsleyani sample 2, every each 1mL of chicken, and medication every day 2 times, is used in conjunction 4d; (the occurring condition of illness) medication after inoculation allantoic fluid 3d of Radix Tetrastigmatis Hemsleyani sample 2 treatment groups, every each 1mL of chicken, medication every day 2 times, is used in conjunction 4d.Radix Tetrastigmatis Hemsleyani sample 3 prevention groups, in inoculation allantoic fluid, start to use Radix Tetrastigmatis Hemsleyani sample 3 oral, every each 1mL of chicken, and medication every day 2 times, is used in conjunction 4d.(the occurring condition of illness) medication after inoculation allantoic fluid 3d of Radix Tetrastigmatis Hemsleyani sample 3 treatment groups, every each 1mL of chicken, medication every day 2 times, is used in conjunction 4d.Western medicine prevention group, in inoculation allantoic fluid, starts to use the ribavirin of 50mg/mL oral, and every each 1mL of chicken, every day 2 times, is used in conjunction 4d.The ribavirin of 50mg/mL for western medicine group, (occurring symptom) medication after inoculation allantoic fluid 3d, every each 1mL of chicken is oral, and medication every day 2 times, is used in conjunction 4d.Infect the chicken inoculation allantoic fluid of matched group, need not any medicine, the results are shown in Table 6.
The prevention of table 6 newcastle disease and therapeutic outcome
Three, the clinical observation of the present invention to newcastle disease treatment
A kind of acute height contagious disease that newcastle disease is caused by Avian pneumo-encephalitis virus, the chicken of various ages in days all can fall ill, and is often septicemia process, principal character is dyspnea, dysentery, and nervous function is disorderly, mucosa and serous coat are hemorrhage, and mortality rate is high, can cause very large economic loss.With Radix Tetrastigmatis Hemsleyani sample 1(, press embodiment 3 methods preparations), Radix Tetrastigmatis Hemsleyani sample 2(is by embodiment 4 methods preparations), Radix Tetrastigmatis Hemsleyani sample 3(presses embodiment 10 methods and prepares), every each oral 1mL of chicken, every day, medication twice, is used in conjunction 4 days.In Suzhou City of Jiangsu Province, Changzhou, Nantong City, Yancheng City carry out Drug therapy test to morbidity chicken, cures the number number evaluation of surviving after by medication 3~4d, the results are shown in Table 7, table 8, table 9.
1 pair of newcastle disease clinical efficacy result unit of table 7 Radix Tetrastigmatis Hemsleyani sample a: %
2 pairs of newcastle disease clinical efficacy result units of table 8 Radix Tetrastigmatis Hemsleyani sample a: %
3 pairs of newcastle disease clinical efficacy result units of table 9 Radix Tetrastigmatis Hemsleyani sample a: %
Visible, Drug therapy newcastle disease more than 1,000 examples of made of the present invention, all obtain good therapeutic effect, after treatment 2d, stop death, after 3~4d, can cure, and after healing, generally do not recur, and show that the present invention treats newcastle disease and has very high curative effect.
Four, the clinical treatment result of the present invention to chicken bursal disease
On April 20th, 2012, Shihhung, Kiangsu Province chicken house, has 220 of 24 Japanese instar chicklings, occurs down in spirits, and feather pine is random, the minimizing of searching for food, fear of cold, normal poly-heap.There is diarrhoea in sick chicken subsequently, discharges white thickness or water sample loose stool.Cut open 4 chickens of inspection, at shank and chest muscle, have hemorrhage; Fabricius bursa edema and hemorrhage, volume increases.With agar-gel precipitation test, be diagnosed as bursal disease.With medication therapy groups 1(of the present invention, by embodiment 3 methods, prepare immediately), medication therapy groups 2(prepares by embodiment 4 methods), medication therapy groups 3(prepares according to embodiment 9 methods), medication therapy groups 4(prepares by embodiment 10 methods) Drug therapy, and be control drug with astragalus polysaccharides, treat altogether 3 days, every each oral 1mL of chickling, medication every day 2 times; Therapeutic outcome is in Table 10.
Table 10 chicken bursal disease clinical efficacy result unit a: %
Five, the clinical treatment result of the present invention to infectious bronchitis of chicken
On May 5th, 2012, Jiangsu Jingjiang chicken house, has 165 of 35 Japanese instar chicklings, occurs suddenly stretching neck, mouth breathing, cough, sneeze, has special rale, and lethargy, wing are sagging, dysentery.Cut open 3 chickens of inspection, bronchus has Catarrhal exudate, and air bag is muddy, and renomegaly, pale is in " the piebaldism kidney " of mottling.With hemagglutination inhibition test, be diagnosed as infectious bronchitis of chicken.With medication therapy groups 1(of the present invention, by embodiment 3 methods, prepare), medication therapy groups 2(prepares by embodiment 4 methods), medication therapy groups 3(prepares according to embodiment 9 methods), medication therapy groups 4(prepares according to embodiment 10 methods) Drug therapy, and be control drug with astragalus polysaccharides, treat altogether 4 days, every each oral 1mL of chickling, medication every day 2 times; Therapeutic outcome is in Table 11.
Table 11 infectious bronchitis of chicken clinical efficacy result unit a: %
Six, the clinical treatment result of the present invention to pig blue-ear disease and circovirus disease
On November 5th, 2011,, there are 77 of 60 age in days piglets on scale pig farm, Jiangsu, occurs that spirit is depressed, loss of appetite or absolutely useless.November 6, body temperature rise to 40.5 ℃ left and right, dyspnea, whole skin is rubescent, diarrhoea, inguinal lymph nodes enlargement.Cut open 5 of inspections, cut open inspection and change: the congested swelling of larynx, lung is point-like and speckle shape is hemorrhage, and tangent plane is marble sample to be changed, and kiney edema, hemorrhage, is khaki.There are a large amount of hydrops in pericardium and thoracic cavity, the heart speckle of bleeding profusely.Mesenteric lymph node, inguinal lymph nodes are hemorrhage.Get pathological material of disease and send academy of agricultural sciences, Jiangsu, use RT-PCR(inverse transcription polymerase chain reaction) detect, be diagnosed as PRRS virus and porcine circovirus mixed infection.With medication therapy groups 1(of the present invention, according to embodiment 5 methods, prepare immediately), medication therapy groups 2(of the present invention prepares according to embodiment 6 methods) and medication therapy groups 3(of the present invention according to embodiment 7 methods, prepare) treatment, and be control drug with astragalus polysaccharides, treat altogether 4 days, every each 5mL of piglet, medication every day 2 times, therapeutic outcome is in Table 12.
Table 12 pig blue-ear disease and porcine circovirus mixed infection clinical efficacy result unit: a %
By clinical practice result, draw, Chinese medicine injection of the present invention and oral liquid treatment chicken bursal disease, infectious bronchitis of chicken, pig blue-ear disease and porcine circovirus mixed infection, curative effect is than astragalus polysaccharides successful.Show Chinese medicine preparation of the present invention, treatment livestock and poultry virus sexually transmitted disease has significant curative effect.
Below in conjunction with specific embodiment, the invention will be further described:
The preparation of embodiment 1 extract 1 of the present invention
Radix Tetrastigmatis Hemsleyani 500g, by 10 extraordinarily after distilled water immersion 6h of medical material weight, puts Decocting pot and endures 1h, leaches medicinal liquid, 10 times of distillation decocting 1h that add again medical material weight, leach medicinal liquid, merge twice medicinal liquid, dehydration is concentrated into thick paste shape, and low-temperature reduced-pressure is dried, and is total to obtain 50g extract.
The preparation of embodiment 2 extract 2 of the present invention
Get Radix Tetrastigmatis Hemsleyani 500g, pulverize, by 10 times of amounts of Radix Tetrastigmatis Hemsleyani powder weight, mass percentage concentration is 80% soak with ethanol after 4 hours, and percolation, reclaims ethanol, is concentrated into thick paste shape, and low-temperature reduced-pressure is dried, and to obtain altogether 30g extract.
The preparation of embodiment 3 oral liquid 1 of the present invention
Radix Tetrastigmatis Hemsleyani 500g, soaks 6h by 8 times of medical material weight, puts Decocting pot and endures 2h, leaches medicinal liquid, then adds 8 times of decocting 2h of medical material weight, leaches medicinal liquid, merges twice medicinal liquid, is concentrated into 500mL, bottling, sealing, sterilizing.
The preparation of embodiment 4 oral liquid 2 of the present invention
Get Radix Tetrastigmatis Hemsleyani 500g, be dissolved in 10 times of Radix Tetrastigmatis Hemsleyani medical material weight amount concentration and be 75% soak with ethanol after 4 hours, percolation, reclaims ethanol, is concentrated into 500mL, bottling, sealing, sterilizing.
The preparation of embodiment 5 injection 1 of the present invention
Radix Tetrastigmatis Hemsleyani 1000g, the 12 distilled water immersion 6h extraordinarily by medical material weight, put Decocting pot and endure 1h, leach medicinal liquid, add again 10 times of decocting 1h, leach medicinal liquid, merge twice medicinal liquid, dehydration is concentrated into thick shape, adding mass percentage concentration is 95% ethanol, makes to reach 60% containing alcohol amount, places after 24h, sucking filtration, reclaims ethanol.It is 95% ethanol that filtrate adds mass percentage concentration again, makes to reach 70% containing alcohol amount, places after 24h, and sucking filtration, reclaims ethanol.By filtrate adding distil water to 800mL.The active carbon that adds 8 grams, boils constant temperature 30min, and filtrate adding distil water, to 1000mL, is heated to 80 ℃, adds 20mL benzyl alcohol, and sucking filtration, to clear and bright, is adjusted pH to 7.0 repeatedly, embedding, and sterilizing 30min, standby.
The preparation of embodiment 6 injection 2 of the present invention
Get Radix Tetrastigmatis Hemsleyani 1000g, pulverize, be dissolved in 10 times of amounts of Radix Tetrastigmatis Hemsleyani medical material weight, concentration is in 85% ethanol, after soaking 4h, filter, residue filters with 85% soak with ethanol 4h again, merges twice filtrate, reclaim ethanol to without alcohol taste, medicinal liquid is concentrated into 800mL, add 8 grams of active carbons, boil constant temperature 30min, sucking filtration, filtrate adding distil water is to 1000mL, be heated to 80 ℃, add 20mL benzyl alcohol, sucking filtration is to clear and bright repeatedly, embedding, sterilizing 30min, standby.
The preparation of embodiment 7 injection 3 of the present invention
Get Radix Tetrastigmatis Hemsleyani 1000g, pulverize, be dissolved in 8 times of amounts of Radix Tetrastigmatis Hemsleyani medical material weight, concentration is in 75% methanol, after soaking 5h, filter, residue soaks 5h with 75% methanol again and filters, and merges twice filtrate, reclaim methanol to without alcohol taste, medicinal liquid is concentrated into 800mL, add 8 grams of active carbons, boil constant temperature 30min, sucking filtration, filtrate adding distil water is to 1000mL, be heated to 80 ℃, add 20mL benzyl alcohol, sucking filtration is to clear and bright repeatedly, embedding, sterilizing 30min, standby.
The preparation of embodiment 8 extract 3 of the present invention
Get Radix Tetrastigmatis Hemsleyani 500g, pulverize, by 10 times of amounts of Radix Tetrastigmatis Hemsleyani powder weight, mass percentage concentration is 75% soak with ethanol after 4 hours, and percolation, reclaims ethanol, is concentrated into thick paste shape, and low-temperature reduced-pressure is dried, and to obtain altogether 32g extract.
The preparation of embodiment 9 oral liquid 3 of the present invention
Get Radix Tetrastigmatis Hemsleyani 500g, be dissolved in 20 times of amount 85% methanol of Radix Tetrastigmatis Hemsleyani medical material weight and soak after 4 hours, percolation, reclaims methanol, is concentrated into 500mL, bottling, sealing, sterilizing.
Embodiment 10
Get Radix Tetrastigmatis Hemsleyani raw medicinal herbs, pulverized 200 orders, with distilled water, be made into every mL medicinal liquid containing the concentration of 1g crude drug.
The preparation of embodiment 11 tablets of the present invention
The Radix Tetrastigmatis Hemsleyani extract preparing according to embodiment 1 method is added to dextrin, mixed pelletization, 60 ℃ of oven dry, cross 20 mesh sieve granulate, tabletting.
Claims (6)
1. the application of Radix Tetrastigmatis Hemsleyani in the medicine of preparation treatment livestock and poultry virus sexually transmitted disease, described livestock and poultry virus sexually transmitted disease is chicken bursal disease, pig blue-ear disease and porcine circovirus mixed infection; Described Radix Tetrastigmatis Hemsleyani comprises the preparation that Radix Tetrastigmatis Hemsleyani raw medicinal herbs, Radix Tetrastigmatis Hemsleyani extract, Radix Tetrastigmatis Hemsleyani processed product or the Radix Tetrastigmatis Hemsleyani of take are active component.
2. application according to claim 1, is characterized in that described Radix Tetrastigmatis Hemsleyani extract is water or extractive with organic solvent.
3. application according to claim 2, is characterized in that described organic solvent is ethanol or methanol.
4. application according to claim 3, is characterized in that described organic solvent is that mass percent concentration is 75%~85% ethanol or methanol.
5. application according to claim 1, is characterized in that described preparation is that Radix Tetrastigmatis Hemsleyani raw medicinal herbs or its extract are directly pulverized and made powder or add pharmaceutically acceptable adjuvant to make oral formulations or injection.
6. application according to claim 5, is characterized in that described oral formulations is mixture, tablet or oral liquid.
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CN1799344A (en) * | 2006-01-06 | 2006-07-12 | 浙江省农业科学院 | Artificial quick propagation method for hemsley rockvine root |
CN101357194A (en) * | 2008-09-11 | 2009-02-04 | 吴国良 | Traditional Chinese medicine for treating Pafeng disease |
CN102258639A (en) * | 2011-09-08 | 2011-11-30 | 南京泽朗医药科技有限公司 | Supercritical carbon dioxide extraction method for tetrastlgma hemsleyanum total flavonoid |
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