CN102887960A

CN102887960A - Lycium barbarum polysaccharide extraction technology

Info

Publication number: CN102887960A
Application number: CN 201110205829
Authority: CN
Inventors: 胡梦媛
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-07-22
Filing date: 2011-07-22
Publication date: 2013-01-23

Abstract

The invention discloses a lycium barbarum polysaccharide extraction technology, belonging to lycium barbarum polysaccharide extraction technologies. The invention provides a lycium barbarum polysaccharide extraction technology. The technology comprises the following steps of: purifying, drying and grinding lycium barbarum; degreasing and decolorizing in petroleum ether with a functional extractor; filtering out the petroleum ether; performing microwave extraction, and filtering the solution; adding anhydrous ethanol into the filtrate for alcohol precipitation; centrifuging the solution at a speed of 8,000r/min for 10min; discarding the supernatant to obtain the precipitate; dissolving the precipitate with distilled water; accurately absorbing the solution; developing and measuring the light absorption value; and calculating the polysaccharide content according to the obtained regression line equation.

Description

A kind of technique of extracting lycium barbarum polysaccharide

Technical field

The invention belongs to the extraction process of lycium barbarum polysaccharide.

Background technology

Wolfberry fruit is traditional conventional Chinese medicine simply, begins to be stated from Shennong's Herbal, classifies as top grade.Compendium of Material Medica is called its kidney tonifying, moistening lung, production of sperm, beneficial gas.Holy Benevolent Prescriptions carries wolfberry fruit wine tonifying deficiency, strengthening the bones and muscles, pleases color, body-building body.Wolfberry fruit is flat, flavor is sweet, have nourishing liver and kidney, the effect of replenishing vital essence to improve eyesight.Modern clinic be widely used in regulate immunologic function, reducing blood-fat, hypoglycemic, protect the liver, antitumor and do old and feeble etc., lycium barbarum polysaccharide all has certain restraining effect to bacterium and mould, to the factor that the above-mentioned functions of wolfberry fruit plays an important role, it is generally acknowledged with wolfberry fruit in the carotenoid, the lycium barbarum polysaccharide (LBP) that exist relevant with trimethyl-glycine isoreactivity material.

The extraction process of existing lycium barbarum polysaccharide mostly is traditional hot water extraction or organic solvent (ether, ethanol, sherwood oil etc.) degreasing after heat water extract method, large, the consuming time length of solvent load and extraction yield are low in these ordinary methods, for improving the economic benefit of wolfberry fruit, improve the extraction yield that effectively becomes to send out in the wolfberry fruit, seeking fast and effectively, the lycium barbarum polysaccharide extracting method is an important subject of matrimony vine industrialized development.

Summary of the invention

The present invention is exactly for the problems referred to above, and a kind of technique of extracting lycium barbarum polysaccharide is provided.

For above-mentioned purpose, technical process of the present invention is:

After the removal of impurities of matrimony vine process, drying, the pulverizing, decolour the elimination sherwood oil at petroleum ether degreasing with the function extractor, again through microwave extraction, solution filter is got filtrate and is added the dehydrated alcohol alcohol precipitation, and solution is centrifugal 10min under the 8000r/min rotating speed, discarding supernatant liquid is precipitated, with the dissolved in distilled water precipitation, accurately draw this solution, develop the color, measure its light absorption value, calculate polysaccharide content according to the gained regression line equation.

Optimal processing parameter is final feed liquid mass volume ratio 1: 10, extraction power 2200W, the extraction time 25min of selecting, and polysaccharide yield is 6.11% under this optimal conditions.

Beneficial effect of the present invention:

The present invention adopts microwave auxiliary extraction technology to extract lycium barbarum polysaccharide, uses the quadrature analysis method to draw the optimum process condition that extracts lycium barbarum polysaccharide; Microwave auxiliary extraction technology is used in the extraction of lycium barbarum polysaccharide, has the advantages such as quick, energy-conservation, safe, high yield and easy amplification easy to control.

Description of drawings

Fig. 1 extraction time is on the impact of microwave extraction polysaccharide.

Fig. 2 is that the feed liquid mass volume ratio is on the impact of microwave extraction polysaccharide yield.

Fig. 3 extracts power to the impact of microwave extraction polysaccharide.

Embodiment

After the removal of impurities of matrimony vine process, drying, the pulverizing, decolour the elimination sherwood oil at petroleum ether degreasing with the function extractor, through microwave extraction, solution filter is got filtrate and is added the dehydrated alcohol alcohol precipitation again, solution is centrifugal 10min under the 8000r/min rotating speed, discard supernatant liquid and be precipitated, with the dissolved in distilled water precipitation, accurately draw this solution, develop the color, measure its light absorption value, calculate polysaccharide content according to the gained regression line equation, calculate as follows polysaccharide yield:

Lycium barbarum polysaccharide yield (%)=(quality of polysaccharide in the filtrate)/(matrimony vine quality) * 100.

Taking by weighing wolfberry fruit powder, is 1: 10 by solid-liquid ratio (g/ml), and extracting power is that 700W carries out microwave extraction, takes a sample every 5min, measures the polysaccharide mass concentration behind the filtration of extracting solution process, the alcohol precipitation, calculates and extracts yield.

The yield of polysaccharide improves along with the increase of the time of microwave exposure as shown in Figure 1, and the trend that extraction time increases in 5～25min the time is obvious, and after the 25min, increase trend is not obvious.When 20～25min, microwave increases the large polysaccharide stripping of destruction of cell walls, extracting yield also increases accordingly, after surpassing 25min, the undue fragmentation of cell walls makes that dissolved impurity is corresponding to be increased, and solvent is almost saturated, and dissolving effectively very much almost stops, yield gets final product because having adopted 25min without obvious increase.

Getting the 20g wolfberry fruit powder, is 700W by extracting power, and extraction time 30min, solid-liquid ratio are respectively and extracted in 1: 8,1: 10,1: 12,1: 15,1: 20, measures the polysaccharide mass concentration behind the filtration of extracting solution process, the alcohol precipitation, calculates and extracts yield.

As can be seen from Figure 2 solid-liquid ratio increases, and polysaccharide extract rate increases, when solid-liquid ratio during at 1: 10 the extraction yield increasing degree large, solid-liquid ratio is after 1: 10, increase trend is not obvious.Material is pure when less than normal, and the concentration difference of effective constituent is on the low side in the solid liquid phase; Along with the increase of solid-liquid ratio, the two-phase concentration difference increases in the leaching process, and mass transfer velocity is accelerated, and the amount of polysaccharide in solvent constantly increases.When solid-liquid ratio was increased to a certain degree, although the concentration difference of effective constituent still exists in the two-phase, mass transfer power increased little, so the extraction yield amplification of polysaccharide is not obvious.Consider that from the effective handy of resource and reduction energy consumption two aspects microwave extraction lycium barbarum polysaccharide solid-liquid ratio should not be too little, also unsuitable excessive, therefore adopt 1: 10 solid-liquid ratio more suitable.

Taking by weighing wolfberry fruit powder, is 1: 10 according to solid-liquid ratio, and extraction time is 30min, and extraction power is respectively 700,1200,1700,2200 and extracts, and measures the polysaccharide mass concentration behind the filtration of extracting solution process, the alcohol precipitation, calculates the extraction yield.

As shown in Figure 3, along with the increasing of microwave extraction power, polysaccharide extracts yield and also increases.Within identical extraction time, extract power and strengthen, the micro-wave energy of material absorbing is more, and the cytoclasis degree is just larger, has in the stripping of active substance, so adopt the extraction power of 2200W.

Taking by weighing wolfberry fruit powder, is 700W by extracting power, and extraction time 30min, solid-liquid ratio extract at 1: 10, repeat to extract 3 times, measure the dense degree of polysaccharide quality, and extracting solution calculates and extracts yield through filtering, measuring the polysaccharide mass concentration behind the alcohol precipitation.

Can know from table 1, increase and extract progression that extract yield and descend rapidly, when extracting 3 times, it is lower that it extracts yield, and the operations such as concentrating and separating in later stage are very difficult, so adopt generally speaking secondary to extract comparatively economical rationality.

On microwave single factor experiment result's basis, Three factors-levels L9 (34) orthogonal test, its level of factor sees Table 2, and each measures the polysaccharide mass concentration after organizing the filtration of extracting solution process, alcohol precipitation, calculate and extract yield, select the processing parameter of microwave extraction lycium barbarum polysaccharide optimum.

Table 1 four factors three horizontal quadratures test L ₉(3 ⁴) result

From orthogonal experiments table 3, can draw the relation of factors and polysaccharide yield: affect order (main → inferior): feed liquid mass volume ratio, extraction power, extraction time.The analysis-by-synthesis various factors is finally selected feed liquid mass volume ratio 1: 10, is extracted power 2200W, extraction time 25min problems such as the impact of polysaccharide yield and production costs, and polysaccharide yield is 6.11% under this optimal conditions.

Claims

1. a technique of extracting lycium barbarum polysaccharide is characterized in that technical process is: after the removal of impurities of matrimony vine process, drying, the pulverizing, decolour at petroleum ether degreasing with the function extractor, the elimination sherwood oil, again through microwave extraction, solution filter, get filtrate and add the dehydrated alcohol alcohol precipitation, solution is centrifugal 10min under the 8000r/min rotating speed, discards supernatant liquid and is precipitated, and precipitates with dissolved in distilled water, accurately draw this solution, develop the color, measure its light absorption value, calculate polysaccharide content according to the gained regression line equation.

2. a kind of technique of extracting lycium barbarum polysaccharide according to claim 1 is characterized in that optimal processing parameter for finally selecting feed liquid mass volume ratio 1: 10, extraction power 2200W, extraction time 25min, and polysaccharide yield is 6.11% under this optimal conditions.