CN102876730A

CN102876730A - Method for promoting fermentation of alcohol in yeasts

Info

Publication number: CN102876730A
Application number: CN2012103043391A
Authority: CN
Inventors: 周雪琴
Original assignee: TAICANG ZHOUSHI CHEMICAL PRODUCT CO Ltd
Current assignee: TAICANG ZHOUSHI CHEMICAL PRODUCT CO Ltd
Priority date: 2012-08-24
Filing date: 2012-08-24
Publication date: 2013-01-16

Abstract

The invention provides a novel method for promoting fermentation of alcohol in yeasts. Bread yeasts are taken as initial strains, and solid matrixes are added in a fermentation medium to research the promoting effects of different solid matrixes on the fermentation of the alcohol in the yeasts.

Description

A kind of promotion yeast alcohol fermentation method

Technical field

The invention belongs to fermentation technical field, be specifically related to a kind of promotion yeast ethanol fermentation novel method.

Background technology

In chemical field, ethanol (being commonly called as alcohol) is a kind of important industrial raw material, be widely used in the fields such as chemical industry, food, drink industry, military project, daily-use chemical industry and medical and health, and ethanol can improve the fuel qualities mouth as a kind of good fuel (its fuel value reaches 26900 kJ/kg).Nowadays, in fermentation industry, mainly adopt the yeast saccharomyces cerevisiae bio-transformation to produce ethanol.

Take biomass resource as raw material, the alcohol fuel of fermentative Production is of great significance for the Double jeopardy tool of alleviating current petroleum resources critical shortage and the deterioration of the ecological environment as renewable and clean energy resource.Yet in the existing ethanol fermentation production technology, the transformation efficiency of raw material and the yield of product are all lower, and high production cost has seriously restricted the alcohol fuel industrial expansion.Therefore, traditional ethanol fermentation technique is transformed, developing low-cost alcohol production new technology seems particularly urgent with the needs that adapt to the fuel ethanol industrial development.

In recent years, high conversion, the ethanol fermentation new technology has received relevant investigator's concern both at home and abroad cheaply.Lipoprotein, chitin, inositol, nutritive salt, nitrogen class nutrition, osmoprotectant, tween-80 and ergosterol have now been confirmed in substratum, to add; adopt the methods such as engineering strain, immobilized cell; raising feed stock conversion that can be in various degree and the yield of product, but these methods have increased again production cost to a certain extent.Found through experiments in the additive that the output that can improve ethanol can reduce production costs again, solid state substrate has feasibility more.

Summary of the invention

The invention provides a kind of promotion yeast ethanol fermentation novel method, the present invention adds solid state substrate take yeast saccharomyces cerevisiae as starting strain in fermention medium, study different solid state substrates to the promoter action of yeast ethanol fermentation.

1. one kind promotes yeast ethanol fermentation novel method, the step that comprises seed culture and fermentation producing and ethanol, when it is characterized in that fermenting, by volume the inoculum size of mark 10% accesses (liquid amount is 100 mL) in the fermention medium with seed liquor, places incubator certain temperature bottom fermentation 42 h.

2. the preparation process of the described seed liquor of step 1: picking one ring bacterial classification from the good fresh inclined-plane of firm switching, access in the 50 mL seed culture mediums, place on the shaking table at 28 ℃, 160 r/min shaking culture 24 h.

3. the described fermention medium of step 1 (g/100 mL): glucose 3-15, yeast powder 0.3, peptone 0.5 and the wheat straw bar, Pericarppium arachidis hypogaeae, rice husk and the sawdust that add respectively 3 g/100 mL are as solid state substrate.

4. the described seed culture medium of step 2 (g/100 mL): glucose 2, yeast powder 0.3, peptone 0.5.

5. the described Wheat Straw of step 3 is optimum additive.

6. the suitableeest addition of the described Wheat Straw of step 3 is 2.0 g/100 mL.

7. the first Sugar concentration of the described the best of step 3 is 12 g/100 mL.

8. the described optimum fermentation temp of step 1 is 32 ℃.

The present invention is with the agriculture and forestry organic waste material of the cheapness solid state substrate as the yeast ethanol fermentation, inquire into different solid state substrates to the impact of yeast ethanol fermentation, set up a kind of high conversion, ethanol fermentation novel method cheaply, for promoting the fast development of fuel ethanol industrial, alleviating current petroleum resources critical shortage provides reference.

Description of drawings

Solid state substrate is on the impact of yeast ethanol fermentation under Fig. 1 condition of different temperatures.

Solid state substrate is on the impact of yeast ethanol fermentation under Fig. 2 condition of different temperatures.

Embodiment

The following examples elaborate to the present invention, but to the present invention without limits.

The used bacterial strain of the present invention is yeast saccharomyces cerevisiae, buys in ACCC, is numbered: ACCC20039

Embodiment 1

The implementation case illustrates different solid state substrates to the impact of yeast ethanol fermentation, adds respectively wheat straw bar, Pericarppium arachidis hypogaeae, rice husk and the sawdust of 3 g/100 mL in fermention medium, behind 42 h that ferment, measures ethanol mass concentration in the fermented liquid, the results are shown in Table 1.

As shown in Table 1, add respectively 4 kinds of different solid state substrates in substratum, the ethanol mass concentration has improved 14.0%～28.7% in the fermented liquid.It is the highest wherein to add in the fermented liquid of Wheat Straw the ethanol mass concentration, has improved 28.7% than the fermented liquid that does not add solid substrate.Different solid state substrates is added in this explanation in substratum all have obvious promoter action to the yeast ethanol fermentation.In 4 kinds of different solid state substrates, Wheat Straw is optimum additive.

Table 1Different solid state substrates are on the impact of ethanol fermentation

Solid substrate	Wheat Straw	Peanut skin	Rice husk	Sawdust	Contrast
						Alcohol concn (g/L)	41.3	36.6	38.2	39.4	32.1

Embodiment 2

The implementation case explanation solid state substrate Different adding amount take Wheat Straw as solid state substrate, is studied its Different adding amount to the impact of yeast ethanol fermentation on the impact of yeast ethanol fermentation, and the result is as shown in table 2.

As can be seen from Table 2, in substratum, add respectively the Wheat Straw of 1～3 g/100 mL, the ethanol mass concentration all has in various degree and to improve in the fermented liquid, wherein adds the fermented liquid that ethanol mass concentration ratio in the fermented liquid of 2.0 g/100 mL Wheat Straws do not add Wheat Straw and has increased by 31.3%.The suitableeest addition that can determine thus Wheat Straw is 2.0 g/100 mL.

Table 2 Wheat Straw Different adding amount is on the impact of ethanol fermentation

Wheat Straw addition (g/L)	1.0	1.5	2.0	2.5	3.0	Contrast
							Alcohol concn (g/L)	40.5	38.6	42.4	39.2	41.3	32.3

Embodiment 3

Solid state substrate is on the impact of yeast ethanol fermentation under the different just Sugar concentration conditions of the implementation case explanation, for studying under the different just Sugar concentration conditions solid substrate to the impact of yeast ethanol fermentation, add respectively 2.0 g/100 mL Wheat Straws in the substratum that contains the first Sugar concentration of 3～15 g/100 mL, the result is shown in Fig. 1.

By Fig. 1 as can be known, in substratum, add solid state substrate and all can improve the ethanol mass concentration.When just Sugar concentration was 12 g/100 mL in the substratum, the ethanol mass concentration had reached maximum value in the fermented liquid, and contrast has improved 24.9%.

Embodiment 4

Solid state substrate is on the impact of yeast ethanol fermentation under the implementation case explanation condition of different temperatures, add 2.0 g/100 mL Wheat Straws at fermention medium, first Sugar concentration is transferred to 12 g/100 mL, solid state substrate is on the impact of yeast ethanol fermentation under the research condition of different temperatures, and experimental result is seen Fig. 2.

Known by Fig. 2, in 30～38 ℃ of scopes, in substratum, add solid state substrate and all can improve the ethanol mass concentration.When leavening temperature was elevated to 32 ℃, the ethanol mass concentration reached maximum value, and contrast has improved 28.8%.

Claims

1. one kind promotes the yeast alcohol fermentation method, comprises the step of seed culture and fermentation producing and ethanol, and when it is characterized in that fermenting, by volume the inoculum size of mark 10% accesses seed liquor in the fermention medium, places incubator certain temperature bottom fermentation 42 h.

2. method according to claim 1, it is characterized in that the preparation process of the seed liquor of seed culture: picking one ring bacterial classification from the good fresh inclined-plane of firm switching, access in the 50 mL seed culture mediums, place on the shaking table at 28 ℃, 160 r/min shaking culture 24 h.

3. method according to claim 1, it is characterized in that fermention medium: glucose 3-15 g/100 mL, yeast powder 0.3 g/100 mL, peptone 0.5 g/100 mL and add respectively 3 g/100 mL be selected from bar, Pericarppium arachidis hypogaeae, rice husk or sawdust as the solid state substrate of additive.

4. method according to claim 2 is characterized in that, seed culture medium: glucose 2 g/100 mL, yeast powder 0.3 g/100 mL, peptone 0.5 g/100 mL.

5. method according to claim 3 is characterized in that, additive is Wheat Straw.

6. method according to claim 5 is characterized in that, the addition of Wheat Straw is 2.0 g/100 mL.

7. method according to claim 3 is characterized in that, first Sugar concentration is 12 g/100 mL.

8. method according to claim 1 is characterized in that, leavening temperature is 32 ℃.