CN102872129B - Application of Houttuynoid E in drug for treating endometrial cancer - Google Patents

Application of Houttuynoid E in drug for treating endometrial cancer Download PDF

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CN102872129B
CN102872129B CN 201210419495 CN201210419495A CN102872129B CN 102872129 B CN102872129 B CN 102872129B CN 201210419495 CN201210419495 CN 201210419495 CN 201210419495 A CN201210419495 A CN 201210419495A CN 102872129 B CN102872129 B CN 102872129B
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houttuynoid
drug
endometrial cancer
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preparing
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CN102872129A (en
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冯怡
黄晨
吴俊华
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Nantong San Intellectual Property Service Co ltd
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Abstract

The invention discloses application of Houttuynoid E in preparing a drug for treating endometrial cancer, belonging to the technical field of the new use of the drug. Houttuynoid E is found to have obvious inhibition effect on the growth of human endometrial cancer cell lines ISK and RL95-2 through MTT (Methyl Thiazolyl Ternzolinm) anticancer activity evaluation in vitro. Therefore, Houttuynoid E can be used for preparing the anti-endometrial cancer drug and has excellent prospects in development and application. The use of Houttuynoid E in preparing the drug for treating the endometrial cancer involved in the invention is disclosed for the first time; the framework type of Houttuynoid E is a brand new framework type; and Houttuynoid E has an unexpectedly high inhibition activity for the endometrial cancer cells.

Description

The application of Houttuynoid E in preparation treatment endometrium cancer drug
Technical field
The present invention relates to the new purposes of compound H outtuynoid E, relate in particular to the application of Houttuynoid E in the anti-endometrium cancer drug of preparation.
Technical background
Cancer is to one of disease of human life's health hazard maximum, and annual have a large amount of people to die from cancer.The research and development of cancer therapy drug are the focuses of study of pharmacy always.Having 74% to be the natural product or derivatives thereof in the antitumor drug, is exactly the reasonable antitumor drug of effect clinically at present as paclitaxel and derivant thereof.Therefore, searching anticancer compound or lead compound have great importance from natural product.
The compound H outtuynoid E that the present invention relates to is one and delivered (Chen in 2012, S. D. et al., 2012. Houttuynoid E_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.) new framework compound, this chemical compound has brand-new framework types, present purposes only relates to anti-herpes simplex virus activity (Chen, S. D. et al., 2012. Houttuynoid E_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.), belong to open first for the purposes of the Houttuynoid E that the present invention relates in preparation treatment endometrium cancer drug, because framework types belongs to brand-new framework types, and its inhibition for endometrial carcinoma cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other chemical compounds, possess outstanding substantive distinguishing features, the control that is used for carcinoma of endometrium simultaneously obviously has obvious improvement.
Summary of the invention
The invention provides the application of compound H outtuynoid E in the preparation antitumor drug.
The present invention adopts following technical scheme: the application of Houttuynoid E in the anti-endometrium cancer drug of preparation, and the structural formula of Houttuynoid E is shown in formula I:
Figure BDA0000231841671
Formula I
The present invention finds that by external MTT anti-tumor activity evaluation the growth of the people's endometrial carcinoma cell of Houttuynoid E strain ISK and RL95-2 also has significant inhibitory effect, suppresses the IC of this 2 strain cell growth 50Value is respectively 0.45 ± 0.07 μ M and 0.11 ± 0.06 μ M.Therefore, Houttuynoid E can have the excellent development application prospect for the preparation of anti-endometrium cancer drug.
The purposes of the Houttuynoid E that the present invention relates in preparation treatment endometrium cancer drug belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for endometrial carcinoma cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other chemical compounds, possess outstanding substantive distinguishing features, the control that is used for carcinoma of endometrium simultaneously obviously has obvious improvement.
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subjected to any restriction of specific embodiment, but limited by claim.
The specific embodiment
The preparation method of compound H outtuynoid E involved in the present invention is referring to document (Chen, S. D. et al., 2012. Houttuynoid E_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.).
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subjected to any restriction of specific embodiment, but limited by claim.
Embodiment 1: the preparation of compound H outtuynoid E tablet involved in the present invention:
Get 20 and digest compound Houttuynoid E, add conventional adjuvant 180 grams of preparation tablet, mixing, conventional tablet machine are made 1000.
Embodiment 2: the preparation of compound H outtuynoid E capsule involved in the present invention:
Get 20 and digest compound Houttuynoid E, add conventional adjuvant such as starch 180 grams of preparation capsule, mixing is encapsulatedly made 1000.
Further specify its pharmaceutically active below by pharmacodynamic experiment.
Experimental example: adopt the growth inhibited effect of the people's endometrial carcinoma cell of mtt assay assessing compound Houttuynoid E strain
1. method: the cell that is in the growth logarithmic (log) phase: people's endometrial carcinoma cell strain ISK and RL95-2(buy from Chinese Academy of Sciences's cell bank) with 1.5 * 10 4The concentration kind is in 96 orifice plates.Cell culture 24 h inhale adherent back and go original culture medium.Test is divided into blank group, drug treating group.Blank group is changed 1640 culture medium that contain 10% hyclone; It is 100 μ M that the replacing of drug treating group contains concentration, 50 μ M, 10 μ M, 1 μ M, 0.1 μ M, the culture medium of the Houttuynoid E of 0.01 μ M and 0.001 μ M.After cultivating 48 h, add the MTT of concentration 5mg/mL, continue to be put in CO 2Incubator is cultivated 4 h, inhale along culture fluid top then and remove 100 μ L supernatants, add 100 μ L DMSO, 10 min are placed in the dark place, utilize microplate reader (Sunrise company product) to measure light absorption value (wavelength 570nm), and according to light absorption value calculating cell survival situation, 6 repeating holes are established in each processing.Cell survival rate (%)=Δ OD Drug treating/ Δ OD Blank* 100.
2. result: the growth of the people's endometrial carcinoma cell of Houttuynoid E strain ISK and RL95-2 has significant inhibitory effect.This chemical compound suppresses the IC of people endometrial carcinoma cell strain ISK and RL95-2 growth 50Value is respectively: 0.45 ± 0.07 μ M and 0.11 ± 0.06 μ M.
Shown that by above-described embodiment the growth of Houttuynoid E of the present invention people's endometrial carcinoma cell strain ISK and RL95-2 has the good restraining effect.Prove that thus Houttuynoid E of the present invention has anti-carcinoma of endometrium activity, can be for the preparation of anti-endometrium cancer drug.

Claims (1)

1.Houttuynoid the application of E in preparation treatment endometrium cancer drug, described compound H outtuynoid E structure as Formula IShown in:
Formula I.
CN 201210419495 2012-10-27 2012-10-27 Application of Houttuynoid E in drug for treating endometrial cancer Active CN102872129B (en)

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Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Houttuynoids A-E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata;Shao-Dan Chen等;《Organic Letters》;20120313;第14卷(第7期);1772-1775 *
Shao-DanChen等.HouttuynoidsA-E Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata.《Organic Letters》.2012

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Contract record no.: X2023980049196

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Nantong Qiaojiang packaging materials Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980049191

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Nantong Bairun Catering Management Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980049185

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Nantong Nongchaoer Food Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048976

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Xingren Tank Farm of Nantong Xinhai Liquefied Gas Service Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048969

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: NANTONG SHUANGHE FOOD Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048967

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Jiangsu guanan electromechanical Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048961

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Nantong Runyuan structural parts Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048952

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231203

Application publication date: 20130116

Assignee: Nantong Jinbao Machinery Manufacturing Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048947

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

Application publication date: 20130116

Assignee: Nantong Juntai motor vehicle testing Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048942

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

Application publication date: 20130116

Assignee: Honglei mechanical equipment (Nantong) Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048782

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

Application publication date: 20130116

Assignee: American Antelope Home Textile (Nantong) Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048774

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

Application publication date: 20130116

Assignee: Nantong Ansheng Textile Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048765

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231130

Application publication date: 20130116

Assignee: NANTONG YA TAI CANDLE ARTS & CRAFTS Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048764

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231130

Application publication date: 20130116

Assignee: Nantong Jianwei Metal Technology Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048759

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

Application publication date: 20130116

Assignee: JIANGSU YATAI FITNESS CO.,LTD.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980048748

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231202

EE01 Entry into force of recordation of patent licensing contract
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20130116

Assignee: Nantong Zhili Machinery Technology Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2023980049698

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20231205

OL01 Intention to license declared
OL01 Intention to license declared
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20130116

Assignee: JIANGSU HAOHAN INFORMATION TECHNOLOGY Co.,Ltd.

Assignor: Nantong San Intellectual Property Service Co.,Ltd.

Contract record no.: X2024980003183

Denomination of invention: Application of Houttuynoid E in the preparation of drugs for the treatment of endometrial cancer

Granted publication date: 20130717

License type: Common License

Record date: 20240322

EE01 Entry into force of recordation of patent licensing contract