CN102872085B - Application of Houttuynoid A in medicines for treating breast cancer - Google Patents
Application of Houttuynoid A in medicines for treating breast cancer Download PDFInfo
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- CN102872085B CN102872085B CN201210418420.2A CN201210418420A CN102872085B CN 102872085 B CN102872085 B CN 102872085B CN 201210418420 A CN201210418420 A CN 201210418420A CN 102872085 B CN102872085 B CN 102872085B
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Abstract
The invention discloses an application of Houttuynoid A in preparing medicines for treating breast cancer, and belongs to the technical field of new applications of medicines. In vitro MTT (Methyl Thiazolyl Tetrazolium) antineoplastic activity evaluation finds that Houttuynoid A has remarkable inhibition effect on the growth of human breast cancer cell lines 4T1, MCF-7, MDA-MB-231 and MCF-7B, so that Houttuynoid A can be used for preparing medicines for inhibiting breast cancer and has good development application prospect. The application of Houttuynoid A in medicines for treating breast cancer is disclosed for the first time, and has extremely high inhibitory activity to breast caner cells as the skeleton type belongs to a completely novel skeleton type.
Description
Technical field
The present invention relates to the new purposes of compound H outtuynoid A, relate in particular to the application of Houttuynoid A in preparing anti-breast cancer medicines.
Technical background
Cancer is to one of disease of human life's health hazard maximum, has a large amount of people to die from cancer every year.The research and development of cancer therapy drug are the focuses of study of pharmacy always.In antitumor drug, have 74% to be natural product or derivatives thereof, if paclitaxel and derivant thereof are exactly the reasonable antitumor drug of effect clinically at present.Therefore, from natural product, find anticancer compound or lead compound has great importance.
The compound H outtuynoid A the present invention relates to is one and within 2012, delivers (Chen, S. D. et al., 2012. Houttuynoid A_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.) New skeleton compound, this compound has brand-new framework types, current purposes only relates to anti-herpes simplex virus activity (Chen, S. D. et al., 2012. Houttuynoid A_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.), purposes for the Houttuynoid A the present invention relates in preparation treatment breast cancer medicines belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for breast cancer cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possesses outstanding substantive distinguishing features, control for breast carcinoma simultaneously obviously has significant progress.
Summary of the invention
The invention provides the application of compound H outtuynoid A in preparing antitumor drug.
The present invention adopts following technical scheme: the application of Houttuynoid A in preparing anti-breast cancer medicines, and the structural formula of Houttuynoid A is as shown in formula I:
Formula I
The present invention finds by the evaluation of external MTT anti-tumor activity, and Houttuynoid A also has significant inhibitory action to the growth of human breast cancer cell strain 4T1, MCF-7, MDA-MB-231 and MCF-7B, suppresses the IC of this 4 strain Growth of Cells
50value is respectively 0.12 ± 0.09 μ M, 0.35 ± 0.06 μ M, 0.23 ± 0.05 μ M and 0.19 ± 0.06 μ M.Therefore, Houttuynoid A can, for the preparation of anti-breast cancer medicines, have good development prospect.
The purposes of the Houttuynoid A the present invention relates in preparation treatment breast cancer medicines belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for breast cancer cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possess outstanding substantive distinguishing features, the control for breast carcinoma simultaneously obviously has significant progress.
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
The specific embodiment
The preparation method of compound H outtuynoid A involved in the present invention is referring to document (Chen, S. D. et al., 2012. Houttuynoid A_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.).
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
Embodiment 1: the preparation of compound H outtuynoid A tablet involved in the present invention:
Get 20 and digest compound Houttuynoid A, add 180 grams of conventional adjuvants preparing tablet, mix, conventional tablet machine is made 1000.
Embodiment 2: the preparation of compound H outtuynoid A capsule involved in the present invention:
Get 20 and digest compound Houttuynoid A, add the conventional adjuvant of preparing capsule as 180 grams of starch, mix, encapsulatedly make 1000.
Below by pharmacodynamic experiment, further illustrate its pharmaceutically active.
Experimental example: adopt the growth inhibited effect of mtt assay assessing compound Houttuynoid A to human breast cancer cell strain
1. method: the cell in growth logarithmic (log) phase: human breast cancer cell strain 4T1, MCF-7, MDA-MB-231 and MCF-7B(buy from Chinese Academy of Sciences's cell bank) with 1.5 * 10
4concentration kind is in 96 orifice plates.After cell culture 24 h are adherent, suck original culture medium.Test is divided into blank group, drug treating group.Blank group is changed 1640 culture medium containing 10% hyclone; It is 100 μ M that drug treating group is changed containing concentration, 50 μ M, 10 μ M, 1 μ M, 0.1 μ M, the culture medium of the Houttuynoid A of 0.01 μ M and 0.001 μ M.Cultivate after 48 h, add the MTT of concentration 5 mg/mL, continue to be put in CO
2incubator is cultivated 4 h, then along culture fluid top, suck 100 μ L supernatants, add 100 μ L DMSO, 10 min are placed in dark place, utilize microplate reader (Sunrise company product) to measure light absorption value (wavelength 570nm), and calculating cell survival situation according to light absorption value, 6 repeating holes are established in each processing.Cell survival rate (%)=Δ OD
drug treating/ Δ OD
blank* 100.
2. result: Houttuynoid A has significant inhibitory action to the growth of human breast cancer cell strain 4T1, MCF-7, MDA-MB-231 and MCF-7B.This compound suppresses the IC of human breast cancer cell strain 4T1, MCF-7, MDA-MB-231 and MCF-7B growth
50value is respectively: 0.12 ± 0.09 μ M, 0.35 ± 0.06 μ M, 0.23 ± 0.05 μ M and 0.19 ± 0.06 μ M.
By above-described embodiment, shown, Houttuynoid A of the present invention has good inhibitory action to the growth of human breast cancer cell strain 4T1, MCF-7, MDA-MB-231 and MCF-7B.Prove thus, Houttuynoid A of the present invention has anti-breast cancer activity, can be for the preparation of anti-breast cancer medicines.
Claims (1)
- The application of 1.Houttuynoid A in preparation treatment breast cancer medicines, described compound H outtuynoid A structure as formula Ishown in:
formula I.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210418420.2A CN102872085B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid A in medicines for treating breast cancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210418420.2A CN102872085B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid A in medicines for treating breast cancer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102872085A CN102872085A (en) | 2013-01-16 |
| CN102872085B true CN102872085B (en) | 2014-04-16 |
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| CN201210418420.2A Active CN102872085B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid A in medicines for treating breast cancer |
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| CN (1) | CN102872085B (en) |
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2012
- 2012-10-27 CN CN201210418420.2A patent/CN102872085B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| Houttuynoids A-E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata;Shao-Dan Chen, et al.;《ORGANIC LETTERS》;20120313;第14卷(第7期);1772-1775 * |
| Shao-DanChen et al..Houttuynoids A-E |
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| CN102872085A (en) | 2013-01-16 |
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