CN102863549A - Method of using by-product fermentation broth generated in production of posaverptidum to produce extracellular posaverptidum - Google Patents
Method of using by-product fermentation broth generated in production of posaverptidum to produce extracellular posaverptidum Download PDFInfo
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Abstract
The invention belongs to the field of microbial pharmacy and particularly relates to a method of using by-product fermentation broth generated in production of posaverptidum to produce extracellular posaverptidum. The by-product fermentation broth generated in liquid fermentation production of coriolus versicolor mycelia is decompressed and condensed and then refrigerated after ethanol solution is added, and precipitates are taken and dried to obtain the extracellular posaverptidum. The method includes the steps: decompressing and condensing the by-product fermentation broth until the specific gravity is 1.02-1.15, adding 80wt%-100wt% ethanol solution into the condensed fermentation broth to enable ethanol content in the mixed solution to reach 70wt%-75wt%, refrigerating the mixed solution for 4-24h at 0-8 DEG C, filtering and subjecting the precipitates to vacuum drying at 60-80 DEG C. The extracellular posaverptidum with immunocompetence is extracted from the by-product fermentation broth generated in production of posaverptidum by means of the ethanol precipitation method, resources are saved, and economic benefits are obtained for enterprises while pollution discharge pressure is relieved.
Description
Technical field
The invention belongs to the microbiological pharmacy field, be specifically related to a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide.
Background technology
According to national standard WS3-228(Z-047)-2003(Z) in the regulation; the polysaccharide-peptide bulk drug is to extract a kind of Chinese medicine that obtains from the liquid fermenting mycelium of Basidiomycetes On Polyporaceae rainbow conk Cov-1 bacterial strain; the energy nourishing the essence and strengthening QI; invigorating spleen and nourishing heart; cellular immune function and blood picture there are certain provide protection, demonstrate,prove for deficiency of both qi and yin, deficiency of both the heart and spleen due to the esophageal carcinoma, cancer of the stomach and the Patients With Primary Lung Cancer Radiotherapy chemotherapy.The activeconstituents of polysaccharide-peptide is in conjunction with protein-polysaccharide, and sugared content is more than or equal to 38.0% in the national Specification polysaccharide-peptide, and protein content is more than or equal to 11.5%, weight-average molecular weight more than or equal to the polysaccharide peptide content of 40kd more than or equal to 50%.After deliberation, the outer glycopeptide of rainbow conk born of the same parents has too prolongs life and strengthens the effect of immunity, and the outer extract 5g/L of rainbow conk born of the same parents has prolonged 36.38% to the mean lifetime of fruit bat.
At present, the production technique of polysaccharide-peptide is solution fermentation, and rainbow conk Cov-1 bacterial strain through the shaking flask shaking culture, is obtained zymocyte liquid behind the aerated culture of seeding tank, breeding tank and fermentor tank, zymocyte liquid is filtered obtain prepared from coriolus versicolor mycelium and fermented liquid again.But the production technique of polysaccharide-peptide has only been utilized prepared from coriolus versicolor mycelium, therefrom extract the Chinese medicine polysaccharide-peptide, and have equally in a large number immunocompetent fermented liquid and be discharged after treatment as waste water containing, in fact contain the compounds such as polysaccharide peptide, phenols, the solid class of plant courage in the fermented liquid, the amino acid of 18 kinds of needed by human body and vitamin V B
1, VB
2, VB
6Reach the active trace element of more than the 10 kind of needed by human body such as copper, iron, potassium, zinc, if discharged like this, both caused huge environmental protection pressure, caused again the unnecessary wasting of resources and financial loss.According to patent of invention 200710042036.6 disclosed methods, in order to reduce heavy metal content, utilize deionized water to substitute tap water fermentative production prepared from coriolus versicolor mycelium and extract polysaccharide-peptide, be lower than the international food hygienic standard with the preparation heavy metal content and limit the quantity of that (British Commonwealth of Nations's food hygienic standard is limited the quantity of: lead is lower than 6.0ppm, and mercury is lower than 0.5ppm, cadmium is lower than 0.1ppm, chromium is lower than 1.0ppm, and antimony is lower than 1.0ppm, and copper is lower than 150ppm, tin is lower than 230ppm, and arsenic is lower than 1.4ppm.) high-quality polysaccharide-peptide, but deionized water is more expensive than tap water, has so just increased the production cost of polysaccharide-peptide.And present polysaccharide-peptide fermentation manufacturing technique also exists the drawback that yield is lower, cost is higher, and this has also caused holding at high price of polysaccharide-peptide bulk drug on the market and polysaccharide-peptide finished product, has increased user's economical load.
Summary of the invention
Extract glycopeptide outside the rainbow conk born of the same parents in the byproduct fermentation liquid that the objective of the invention is from produce the polysaccharide-peptide process, to produce, avoided the wasting of resources and financial loss, and the production cost of the polysaccharide-peptide of employing this law production is low, yield is high.
Technical scheme of the present invention is as follows:
Add ethanolic soln and refrigeration after liquid fermenting being produced the byproduct fermentation liquid concentrating under reduced pressure that produces in the prepared from coriolus versicolor mycelium process, get its drying precipitate.
It is 1.02-1.15 that described byproduct fermentation liquid is evaporated to proportion.
In concentrated broth, add the ethanolic soln of 80wt%-100wt%, and make the ethanol content in the mixed solution reach 70wt%-75wt%.
Described refrigerating temperature is 0 ℃-8 ℃, and cold preservation time is 4-24 hour.
Be vacuum-drying under 60 ℃ of-80 ℃ of conditions with throw out in temperature.
Rainbow conk Cov-1 bacterial strain with activation, process slant culture, shake-flask culture, level liquid cultivation and secondary liquid culture to the step by step enlarged culturing method of fermentation culture obtains zymocyte liquid, will obtain prepared from coriolus versicolor mycelium and byproduct fermentation liquid after the zymocyte liquid filtration again.Wherein the yield of prepared from coriolus versicolor mycelium is more than every liter of zymocyte liquid 20g.Described level liquid substratum, secondary liquid nutrient medium and the employed carbon source of fermention medium are one or more in glucose, sucrose, Semen Maydis powder, wheat-flour, starch or the dextrin; Nitrogenous source is one or more in soybean cake powder, groundnut meal or the yeast powder; One or more in dipotassium hydrogen phosphate, calcium carbonate or the sal epsom of its inorganic salt; The carbon nitrogen source total amount of described level liquid substratum is the 3.5%-5% of this level liquid substratum, the carbon nitrogen source total amount of described secondary liquid nutrient medium is the 3.5%-5% of this secondary liquid nutrient medium, and the carbon nitrogen source total amount of described fermention medium is the 6%-8% of this fermention medium.The inoculum size of described every grade of liquid fermenting is the 5%-10% of this grade liquid fermentation medium, and culture temperature is 25 ℃-30 ℃, and air flow is 0.5v/(vmin)-1.5v/(vmin), fermentation time is 48 hours-72 hours.The fermentation water that uses in the above prepared from coriolus versicolor mycelium fermenting process all adopts tap water.
Described prepared from coriolus versicolor mycelium obtains polysaccharide-peptide through water extraction and alcohol precipitation method, comprises the steps,
(1) be that the pure water that prepared from coriolus versicolor mycelium 2-4 doubly measures cleans prepared from coriolus versicolor mycelium and filtration with weight; Be that prepared from coriolus versicolor mycelium 3-5 pure water doubly extracted 2 hours-6 hours under 90 ℃ of-98 ℃ of temperature with weight; Separate and obtain extracting solution.
(2) behind concentrating under reduced pressure, adopt alcohol deposition method to be precipitated thing the extracting solution in the step (1), and get its drying.The yield of this polysaccharide-peptide is more than every liter of zymocyte liquid 2g.
Alcohol deposition method in the described step (2) is: add the 80wt%-95wt% ethanolic soln in the concentrated solution of extracting solution, and make the ethanol content in the mixed solution reach 70wt%-75wt%, mixed solution is placed refrigeration 4 hours-24 hours and filtration under 0 ℃ of-8 ℃ of condition, with the polysaccharide-peptide throw out 60 ℃ of-80 ℃ of vacuum-dryings, crushing screening namely gets polysaccharide-peptide.
The invention has the beneficial effects as follows: the present invention adopts alcohol deposition method extraction from the byproduct fermentation liquid of producing polysaccharide-peptide to have glycopeptide outside the immunocompetent rainbow conk born of the same parents, has saved resource, for enterprise has obtained economic return; When adopting solution fermentation to produce prepared from coriolus versicolor mycelium, by the carbon nitrogen total amount in the increase substratum and the regulation and control of carbon-nitrogen ratio thereof, significantly improved the yield of the outer glycopeptide of polysaccharide-peptide and rainbow conk born of the same parents, wherein, the yield of polysaccharide-peptide is increased to more than the 2g/L from original 1g/L; The present invention only adopts pure water in the extraction stage of polysaccharide-peptide, the water source in other stages all adopts tap water, produce thus the polysaccharide-peptide that obtains and meet the international food hygienic standard fully, all use deionized water more economical, economical, convenient than prior art is disclosed in fermentation and extraction whole process, reduced the production cost of enterprise.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
1) production process of polysaccharide-peptide
Get the rainbow conk Cov-1 inoculation of activation in PDA solid slant culture base, cultivated 6 days under 28 ℃ of conditions, obtain the rainbow conk slant strains; With the rainbow conk slant strains, be inoculated in the shaking flask liquid nutrient medium that is formed by 2% glucose, 4% Semen Maydis powder, under 28 ℃ of conditions, to cultivate 6 days, the shaking flask rotating speed is 200 rev/mins, obtains rainbow conk shaking flask bacterial classification; Get rainbow conk shaking flask bacterial classification 2L, be inoculated in the 50L seeding tank that 40L level liquid substratum is housed, wherein, the level liquid bacterium culture medium contains 1% glucose, 2% Semen Maydis powder, 1% dextrin, 1% soybean cake powder, 0.075% sal epsom and 0.1% dipotassium hydrogen phosphate, air flow is 0.5v/(vmin), cultivated 48 hours under 28 ℃ of conditions, get rainbow conk level liquid bacterial classification; Get rainbow conk level liquid bacterial classification 40L, be inoculated in 1 ton of seeding tank that 400L secondary liquid nutrient medium is housed, wherein the secondary liquid nutrient medium is comprised of 1% glucose, 2% Semen Maydis powder, 1% dextrin, 1% soybean cake powder, 0.075% sal epsom and 0.1% dipotassium hydrogen phosphate, air flow is 0.5v/(vmin), cultivated 48 hours under 28 ℃ of conditions, get the rainbow conk second-class liquid isolate; Get rainbow conk second-class liquid isolate 400L, be inoculated in 5 tons of fermentor tanks that 4 tons of fermention mediums are housed, wherein fermention medium contains 2% glucose, 2% Semen Maydis powder, 3% dextrin, 1% soybean cake powder, 0.05% sal epsom and 0.1% dipotassium hydrogen phosphate, 0-36 hour air flow is 0.5v/(vmin), 36-72 hour air flow is 1.5v/(vmi n), cultivated 72 hours under 28 ℃ of conditions, the press filtration of plate basket gets prepared from coriolus versicolor mycelium; Filtrate is byproduct fermentation liquid.Be tap water with the top fermentation water.
Be that the pure water of 3 times of amounts of prepared from coriolus versicolor mycelium cleans prepared from coriolus versicolor mycelium once with weight, the press filtration of plate basket is the pure water of 4 times of prepared from coriolus versicolor mycelium with weight, and extraction is 4 hours under 98 ℃ of conditions.The press filtration of plate basket, the extracting solution that is separated to is evaporated to proportion under 70 ℃ of conditions be 1.10, slowly add 100wt% ethanol, and make the ethanol content in the mixed solution reach 75wt%, with refrigeration under 4 ℃ of conditions of mixture 12 hours, then filter and obtain polysaccharide-peptide precipitation, 70 ℃ of vacuum-dryings, crushing screening namely gets polysaccharide-peptide.
2) production process of the outer glycopeptide of rainbow conk born of the same parents
It is 1.02 that the byproduct fermentation liquid that produces in the liquid fermenting prepared from coriolus versicolor mycelium process is evaporated to proportion in 60 ℃, slowly add 100wt% ethanol, and make the ethanol content in the mixed solution reach 75wt%, and mixed solution is refrigerated 4 hours under 0 ℃ of condition, filter to get the outer glycopeptide precipitation of rainbow conk born of the same parents.60 ℃ of vacuum-dryings, crushing screening namely gets the outer glycopeptide of rainbow conk born of the same parents.
Yield according to weighting method statistics prepared from coriolus versicolor mycelium, polysaccharide-peptide and the outer glycopeptide of rainbow conk born of the same parents, according to national standard WS3-228(Z-047)-2003(Z) carry out main Quality Index Analysis, measure heavy metal content according to British Commonwealth of Nations's food hygienic standard, the yield of prepared from coriolus versicolor mycelium is every liter of zymocyte liquid 24.6g as a result, and the yield of polysaccharide-peptide is every liter of zymocyte liquid 2.4g.Sugared content is 39.8% in the polysaccharide-peptide, protein content is 24.6%, weight-average molecular weight accounts for 56.7% more than or equal to 40Kd's, and lead content is 0.222ppm, and cadmium content is 0.085ppm, chromium content is 0.760ppm, antimony content is 0.507ppm, and copper content is 35.217ppm, and arsenic content is 0.304ppm, mercury does not detect, and tin does not detect.The yield of the outer glycopeptide of rainbow conk born of the same parents is every liter of zymocyte liquid 12.6g.Sugared content is 46.8% in the outer glycopeptide of rainbow conk born of the same parents, and protein content is 18.4%.
Embodiment 2
1) production process of polysaccharide-peptide
Get the rainbow conk Cov-1 inoculation of activation in PDA solid slant culture base, cultivated 7 days under 28 ℃ of conditions, get the rainbow conk slant strains; With the rainbow conk slant strains, be inoculated in the shaking flask liquid nutrient medium that is formed by 1.5% glucose, 4% Semen Maydis powder, under 25 ℃ of conditions, to cultivate 7 days, the shaking flask rotating speed is 180 rev/mins, obtains rainbow conk shaking flask bacterial classification; With rainbow conk shaking flask bacterial classification 2L, be inoculated in the 50L seeding tank that 40L level liquid substratum is housed, the level liquid substratum contains 1% glucose, 2% wheat-flour, 0.5% yeast powder, 0.05% sal epsom and 0.05% dipotassium hydrogen phosphate, air flow is 0.5v/(vmin), cultivated 36 hours under 25 ℃ of conditions, get rainbow conk level liquid bacterial classification; Get rainbow conk level liquid bacterial classification 40L, be inoculated in 1 ton of seeding tank that 400L secondary liquid nutrient medium is housed, the secondary liquid nutrient medium contains 2% glucose, 2% wheat-flour, 0.5% yeast powder, 0.05% sal epsom and 0.05% dipotassium hydrogen phosphate, air flow is 0.5v/(vmin), cultivated 36 hours under 25 ℃ of conditions, get the rainbow conk second-class liquid isolate; Get rainbow conk second-class liquid isolate 400L, be inoculated in 5 tons of fermentor tanks that 4 tons of fermention mediums are housed, fermention medium contains 3% glucose, 2% Semen Maydis powder, 2% soybean cake powder, 0.05% sal epsom and 0.1% dipotassium hydrogen phosphate, air flow is 1.0v/(vmin), cultivated 60 hours under 25 ℃ of conditions, the press filtration of plate basket gets prepared from coriolus versicolor mycelium; Filtrate is byproduct fermentation liquid.Be tap water with the top fermentation water.
Be that the pure water of 4 times of amounts of prepared from coriolus versicolor mycelium cleans prepared from coriolus versicolor mycelium once with weight, the press filtration of plate basket is the pure water of 3 times of prepared from coriolus versicolor mycelium with weight, and extraction is 2 hours under 94 ℃ of conditions.The press filtration of plate basket, the extracting solution that is separated to is evaporated to proportion under 60 ℃ of conditions be 1.02, slowly add 90wt% ethanol, and make the ethanol content in the mixed solution reach 73wt%, with refrigeration under 0 ℃ of condition of mixture 4 hours, then filter and obtain polysaccharide-peptide precipitation, 60 ℃ of vacuum-dryings, crushing screening namely gets polysaccharide-peptide.
2) production process of the outer glycopeptide of rainbow conk born of the same parents
It is 1.10 that the byproduct fermentation liquid that produces in the liquid fermenting prepared from coriolus versicolor mycelium process is evaporated to proportion in 70 ℃, slowly add 90wt% ethanol, and make the ethanol content in the mixed solution reach 72wt%, and mixed solution is refrigerated 12 hours under 4 ℃ of conditions, filter to get the outer glycopeptide precipitation of rainbow conk born of the same parents.70 ℃ of vacuum-dryings, crushing screening namely gets the outer glycopeptide of rainbow conk born of the same parents.
Analytical results is as follows: the yield of prepared from coriolus versicolor mycelium is every liter of zymocyte liquid 20.5g, and the yield of polysaccharide-peptide is every liter of zymocyte liquid 2.1g.Sugared content is 41.4% in the polysaccharide-peptide, protein content is 24.3%, weight-average molecular weight accounts for 53.1% more than or equal to 40Kd's, and lead content is 0.690ppm, and cadmium content is 0.180ppm, chromium content is 0.391ppm, antimony content is 0.156ppm, and copper content is 5.343ppm, and arsenic content is 1.030ppm, mercury does not detect, and tin does not detect.The yield of the outer glycopeptide of rainbow conk born of the same parents is every liter of zymocyte liquid 11.2g.The sugared content of the outer glycopeptide of rainbow conk born of the same parents is 51.9%, and protein content is 19.5%.
Embodiment 3
Get the rainbow conk Cov-1 inoculation of activation in PDA solid slant culture base, cultivated 5 days under 30 ℃ of conditions, get the rainbow conk slant strains; With the rainbow conk slant strains, be inoculated in the shaking flask liquid nutrient medium that is formed by 2% glucose, 3% Semen Maydis powder, under 30 ℃ of conditions, to cultivate 5 days, the shaking flask rotating speed is 220 rev/mins, obtains rainbow conk shaking flask bacterial classification; With rainbow conk shaking flask bacterial classification 2L, be inoculated in the 50L seeding tank that 40L level liquid substratum is housed, the level liquid substratum contains 1% sucrose, 1% Semen Maydis powder, 1.5% groundnut meal, 0.5% yeast powder, 0.1% sal epsom and 0.075% dipotassium hydrogen phosphate, air flow is 1.0(v/vmin), cultivated 42 hours under 30 ℃ of conditions, get rainbow conk level liquid bacterial classification; Get rainbow conk level liquid bacterial classification 40L, be inoculated in 1 ton of seeding tank that 400L secondary liquid nutrient medium is housed, the secondary liquid nutrient medium contains 1% sucrose, 1% starch, 1% soybean cake powder, 0.5% groundnut meal, 0.1% calcium carbonate and 0.05% dipotassium hydrogen phosphate, air flow is 1.0v/(vmin), cultivated 42 hours under 30 ℃ of conditions, get the rainbow conk second-class liquid isolate; Get rainbow conk second-class liquid isolate 400L, be inoculated in 5 tons of fermentor tanks that 4 tons of fermention mediums are housed, fermention medium is comprised of 1.5% Semen Maydis powder, 1% sucrose, 2% starch, 1.5% groundnut meal, 0.05% sal epsom and 0.1% dipotassium hydrogen phosphate, 0-24 hour air flow is 0.5v/(vmin), 24-48 hour air flow is 1.0v/(vmin), cultivated 48 hours under 30 ℃ of conditions, the press filtration of plate basket gets prepared from coriolus versicolor mycelium.Be tap water with the top fermentation water.
Be that the pure water of 2 times of amounts of prepared from coriolus versicolor mycelium cleans prepared from coriolus versicolor mycelium once with weight, the press filtration of plate basket is the pure water of 5 times of prepared from coriolus versicolor mycelium with weight, and extraction is 6 hours under 90 ℃ of conditions.The press filtration of plate basket, the extracting solution that is separated to is evaporated to proportion under 80 ℃ of conditions be 1.15, slowly add 80wt% ethanol, and make the ethanol content in the mixed solution reach 70wt%, with refrigeration under 8 ℃ of conditions of mixture 24 hours, then filter and obtain polysaccharide-peptide precipitation, 80 ℃ of vacuum-dryings, crushing screening namely gets polysaccharide-peptide.
2) production process of the outer glycopeptide of rainbow conk born of the same parents
It is 1.15 that the byproduct fermentation liquid that produces in the liquid fermenting prepared from coriolus versicolor mycelium process is evaporated to proportion in 80 ℃, slowly add 80wt% ethanol, and make the ethanol content in the mixed solution reach 70wt%, mixed solution is refrigerated 24 hours under 8 ℃ of conditions, filter to get the outer glycopeptide precipitation of rainbow conk born of the same parents, 80 ℃ of vacuum-dryings, crushing screening namely gets the outer glycopeptide of rainbow conk born of the same parents.
Analytical results is as follows: the yield of prepared from coriolus versicolor mycelium is every liter of zymocyte liquid 22.4g, and the yield of polysaccharide-peptide is every liter of zymocyte liquid 2.4g.Sugared content is 40.3% in the polysaccharide-peptide, protein content is 25.1%, weight-average molecular weight accounts for 64.9% more than or equal to 40Kd's, and lead content is 0.560ppm, and cadmium content is 0.120ppm, chromium content is 0.464ppm, antimony content is 0.115ppm, and copper content is 3.720ppm, and arsenic content is 0.870ppm, mercury does not detect, and tin does not detect.The yield of the outer glycopeptide of rainbow conk born of the same parents is every liter of zymocyte liquid 10.3g.Sugared content is 52.8% in the outer glycopeptide of rainbow conk born of the same parents, and protein content is 17.6%.
Claims (8)
1. method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide, it is characterized in that: add ethanolic soln and refrigeration after liquid fermenting being produced the byproduct fermentation liquid concentrating under reduced pressure that produces in the prepared from coriolus versicolor mycelium process, get its drying precipitate.
2. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 1, it is characterized in that: it is 1.02-1.15 that described byproduct fermentation liquid is evaporated to proportion.
3. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 1, it is characterized in that: in concentrated broth, add the ethanolic soln of 80wt%-100wt%, and make the ethanol content in the mixed solution reach 70wt%-75wt%.
4. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 1, it is characterized in that: described refrigerating temperature is 0 ℃-8 ℃, and cold preservation time is 4-24 hour.
5. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 1 is characterized in that: be vacuum-drying under 60 ℃ of-80 ℃ of conditions with throw out in temperature.
6. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 1, it is characterized in that: the acquisition process of described prepared from coriolus versicolor mycelium and fermented liquid is the rainbow conk Cov-1 bacterial strain with activation, obtain zymocyte liquid and filtration through slant culture, shake-flask culture, level liquid cultivation, secondary liquid culture to the step by step enlarged culturing method of fermentation culture, namely obtain prepared from coriolus versicolor mycelium and byproduct fermentation liquid.
7. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 6, it is characterized in that: described level liquid substratum, secondary liquid nutrient medium and the employed carbon source of fermention medium are one or more in glucose, sucrose, Semen Maydis powder, wheat-flour, starch or the dextrin, and nitrogenous source is one or more in soybean cake powder, groundnut meal or the yeast powder; The carbon nitrogen source total amount of described level liquid substratum is the 3.5%-5% of this level liquid substratum, the carbon nitrogen source total amount of described secondary liquid nutrient medium is the 3.5%-5% of this secondary liquid nutrient medium, and the carbon nitrogen source total amount of described fermention medium is the 6%-8% of this fermention medium.
8. a kind of method of utilizing the outer glycopeptide of byproduct fermentation liquid production rainbow conk born of the same parents that produces when producing polysaccharide-peptide according to claim 6, it is characterized in that: described prepared from coriolus versicolor mycelium obtains polysaccharide-peptide through water extraction and alcohol precipitation method, comprise the steps,
(1) be that the pure water that prepared from coriolus versicolor mycelium 2-4 doubly measures cleans prepared from coriolus versicolor mycelium and filtration with weight; Be that prepared from coriolus versicolor mycelium 3-5 pure water doubly extracted 2-6 hour under 90 ℃ of-98 ℃ of temperature with weight; Separate and obtain extracting solution.
(2) behind concentrating under reduced pressure, adopt alcohol deposition method to be precipitated thing the extracting solution in the step (1), and get its drying.
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| CN104262503A (en) * | 2014-10-23 | 2015-01-07 | 广东岭南职业技术学院 | Extraction and stepped purification process for polysaccharopeptide |
| CN112876533A (en) * | 2021-01-18 | 2021-06-01 | 江苏神华药业有限公司 | Preparation method of polysaccharopeptide |
| CN113150063A (en) * | 2021-01-18 | 2021-07-23 | 江苏神华药业有限公司 | Preparation method of novel polysaccharopeptide |
| CN113957109A (en) * | 2021-10-28 | 2022-01-21 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262503A (en) * | 2014-10-23 | 2015-01-07 | 广东岭南职业技术学院 | Extraction and stepped purification process for polysaccharopeptide |
| CN112876533A (en) * | 2021-01-18 | 2021-06-01 | 江苏神华药业有限公司 | Preparation method of polysaccharopeptide |
| CN113150063A (en) * | 2021-01-18 | 2021-07-23 | 江苏神华药业有限公司 | Preparation method of novel polysaccharopeptide |
| CN113957109A (en) * | 2021-10-28 | 2022-01-21 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
| CN113957109B (en) * | 2021-10-28 | 2022-09-06 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
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