CN102844121A - Centrifuge tube - Google Patents
Centrifuge tube Download PDFInfo
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- CN102844121A CN102844121A CN2010800554604A CN201080055460A CN102844121A CN 102844121 A CN102844121 A CN 102844121A CN 2010800554604 A CN2010800554604 A CN 2010800554604A CN 201080055460 A CN201080055460 A CN 201080055460A CN 102844121 A CN102844121 A CN 102844121A
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- tube
- intervalve
- centrifugal separating
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- separating tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
Abstract
The present invention relates to a centrifuge tube for use in the centrifugation of biological materials, wherein the diameter of the centrifuge tube is improved to enable minute centrifugation, and in which a thin, weak, and narrow portion of the centrifuge tube, to which a strong force is to be applied, is provided with a support piece to enable centrifugation to be performed without causing a deformation in the centrifuge tube. The centrifuge tube of the present invention enables the centrifugation of materials using differences in the specific gravities of the materials to be easily performed without using a reagent or the like. The centrifuge tube of the present invention has a closure made of a material such as a rubber, polyethylene, polypropylene, silicon or the like, such that a needle may penetrate through the closure and the centrifuge tube can be protected from contamination caused by bacteria, and centrifugation of biological materials can be performed in a closed space.
Description
Technical field
The present invention relates to a kind of centrifugal separating tube, relate in particular to the centrifugal separating tube that easy separation goes out specific composition.
Background technology
Not only for species analysis, also for the cultivation of cell and affirmation and the amplification of DNA, biological agents be separated in that all biological heredities are learned or medically, the operation of basic property of can saying so.For the regenerative medicine that utilizes stem cell, emulative research is all being done in the whole world in the present medical science.In order to obtain this type of stem cell, following stem cell is arranged just.The adult stem cell (adult stem cell) that promptly on the embryonic stem cell that obtains on the blastaea (Blstocyst) of early period of origination (embryonic stem cell) and adult after generating process finishes or placenta, obtains.
The method of wherein obtaining adult stem cell mainly contains 3 kinds.The 1st kind is to obtain through marrow (bone marrow), and the 2nd kind is to obtain through Cord blood (umbilical cord blood), and the 3rd kind is to obtain easily through peripheral blood (mesenchymal blood).And said all methods at first all will be passed through centrifugal separation processes, after isolating great achievement in the blood and dividing red blood cell, leucocyte, blood platelet blood plasma, carry out the operation of the stem cell of separating monocytic cell group again.We examine or check existing centrifugation rule for as follows in this process.
Fig. 1 is an exemplary plot of existing centrifugal separating tube.The blood that the solution that mixes according to various materials particularly extracts the mankind or animal centrifugalizes then; Red blood cell in the blood; Leucocyte; Blood platelet and other plasma fractions are because each intrinsic difference in specific gravity, are separated layer by layer by sinking to light order by weight to the upper strata from the lower floor of centrifugal separating tube 10.
At first move on to centrifugal separating tube to the peripheral blood that extracts through injection-tube.This process need: open the bottle stopper of centrifugal separating tube, injection needle be positioned at the rear flank of centrifugal separating tube, with the peripheral blood injection in the injection-tube in centrifugal separating tube.Thereafter the bottle stopper of centrifugal separating tube beyond the Great Wall after removing needle tubing from centrifugal separating tube.The bucket that centrifugal separating tube is placed on centrifugal separating tube goes up that needed time and rotary speed (rpm) are set in the back or acceleration of gravity (g) makes its running then.Usually red blood cell occupies proportion the heaviest in the blood, so be formed on lower floor, red blood cell accounts in the blood 47% during the male sex, accounts for 42% during the women.What occupy the red blood cell upper strata is leucocyte and blood platelet, accounts for the about 1% of all blood specific gravitys, also comprises the monocyte of peripheral hematopoietic stem cells here.Remaining composition occupies the superiors, is serum and the fibrinogen that is called blood plasma.Centrifugation finishes the back and takes out centrifugal separating tube, opens bottle stopper and isolates through the difference in specific gravity of constitute through suction pipe or hair and blood pipe or needle tubing then and come isolated desired substance, and remaining composition can be preserved in addition and also can discard also and can utilize again.
In said operating process, open or close during the bottle stopper process or the process of injection blood, foreign matters such as the virus in the ambient air or other bacteriums may enter in the centrifugal separating tube, again can the contaminated culture of generation through cultivating.And, observe the centrifugal separating tube that uses this moment and find that diameter narrows down substantially gradually from top to bottom.If required composition is in just easy separation of downside, but if required composition is in centre position rather than downside according to proportion in all solution, or when being in the position of the centrifugal separating tube diameter broad of upside more, just can not carry out trickle separation.Particularly the buffy coat that contains stem cell (buffy coat) that will separate only account for all solution and be no more than 1% degree; Therefore; In fact if on the wide centrifugal separating tube of diameter, separate; Its separating layer is formed on erythrocytic upper strata as paper sheet thickness, even the expert also is difficult to it is carried out lock out operation.
Fig. 2 is the exemplary plot that the blood taking bag that is called bags of blood usually is shown, and centrifugalizes after will taking care of for the moment through the blood that blood sampling is collected, and blood is become to isolate respectively red blood cell, leucocyte, blood platelet and blood plasma by each.When carrying out leucocyte or blood platelet separation through said two kinds of containers; Only account for this composition of all blood 1% degree; Carrying out just can forming the thin buffy coat that has white after 1 centrifugation, isolate this type of buffy coat, is very difficult problem on actual clinical.
Fig. 3 is the sectional view that existing other centrifugal separating tube is shown, and for the diameter of centrifugal separating tube 10, the diameter of intervalve 40 is obviously littler compared with other two pipes 30,50.Buffy coat is formed on when being designed to so thin intervalve 40, and compared with being formed on other two pipes 30,50, its separation is easier.But because the ratio that red blood cell occupies in said all blood is variant according to men and women or individual; Therefore; In order buffy coat to be moved on the intervalve 40; Form other plug 60, or form the other block 70 that surrounds centrifugal separating tube 10, the strong gravity acceleration g that produces when preventing to centrifugalize causes that the thin intervalve of diameter 40 deforms.
In the US patent 3,513,976; In order to obtain leucocyte from peripheral blood, the middle part of centrifugal separating tube is formed like neck with a tight waist, in the pipe of downside, can assemble red blood cell; Again in order to assemble leucocyte at the neck position, at one side formation hole or other pipes of following side pipe.But this mode manufacturing expense is expensive and operation inconvenience.
The US patent 4,861 again, in 477, in order to solve said problem, form plug at the downside of centrifugal separating tube, adjust the volume in the space that red blood cell separates.For the problem points that the centrifugal separating tube that solves mid portion with a tight waist is out of shape owing to strong acceleration of gravity, constitute thing at outside other blocks that form of centrifugal separating tube, but this mode is in the cost of manufacture that increases centrifugal separating tube.
The US patent 5 again; 422; In 018, adopt through in the middle of umbilicate inner centrifugal separating tube and the outside centrifugal separating tube that surrounds this inner centrifugal separating tube, filling medium and protect the mode of intervalve of the minor diameter of centrifugal separating tube, but this type of mode is operated inconvenience and increased cost of manufacture.
Among the Korea S publication 10-2006-0059849, openly be included in the preparation method of adding the blood platelet blood plasma (platelet rich plasma) of water soluble polymer engineering in the peripheral blood.Through similar method, in order from peripheral blood, to isolate monocyte, use the Ficoll-Hypaque of the polymer of ficoll (Ficoll) and cardiografin (polymer of sodium Ditrizote) in the laboratory.The proportion of this Ficoll-Hypaque is 1.0778g/ml, and is light compared with monocyte, and heavier than red blood cell, therefore, can utilize its difference in specific gravity to separate.If when using described polymer, put washing into and remove the washing process that sinks to the extracellular aMEM bottom the taper centrifuge tube through just passing through after the 200xg centrifugation with interior 10 minutes of aMEM (alpha miniumessential Medium Jaeil Biotech Service Korea).
Peripheral blood or other biological are learned the solid constituent that is contained in the liquid of material and all kinds owing to respectively its difference in specific gravity is arranged, and therefore, utilize the method for this difference in specific gravity to possess business efficiency most in the centrifugal separation.
Utilize this trickle difference in specific gravity; And its micro constitutent is in the goods at position of the narrower of centrifugal separating tube; But this based article is made inconvenience and contaminated danger is arranged; Use also inconvenient, according to attachment device or the problem of using expensive material production cost to uprise, and the conical tube of nearest widely used 15ml and 50ml amount and being not suitable on the bucket of centrifugal separating tube.
Patent documentation
3,513, No. 976 (W.C.James) 1970.5.26 of document 1 US
4,861, No. 477 (Shiro Kimura) 1989.8.29 of document 2 US
5,422, No. 018 (outer 2 people of Alexander M.Saunders) 1995.6.6 of document 3 US
Document 4 KR 10-0,409,028 (Jeon Hong Jea) 2003.11.27
Document 5 KR 10-0,489,248 (Medipost Corp) 2005.5.11
Document 6 KR 10-2006-0,059,849 (liking to be beautiful in the field, angle) 2006.6.2
Document 7 US 6,835,353 (outer 2 people of Emery Smith) 2004.12.28
Summary of the invention
The present invention provides a kind of centrifugal separating tube, and this centrifugal separating tube is from going out specific composition as easy separation the blood sample of centrifugation object.
The present invention provides a kind of centrifugal separating tube, owing to contain biology chemistry property composition such as peripheral blood in the solution, the difference in specific gravity of utilizing its each material is separated component effectively.
Especially; Medical science life engineering ties up in separation, cultivation and the regeneration of the cell of research recently; What provide a kind of utensil to be breaking to take place in its separation process comprises biology in interior pollution etc., and can economy and safety, isolates biological agents efficiently.
The present invention relates to a kind of outer wall that possesses the carrying current substance in the inner space; The stream material that is contained in inside is carried out the centrifugal separating tube of component separating according to its proportion; Said centrifugal separating tube possesses: opening is arranged at top, and possesses the upper tube of predetermined internal capacity; The intervalve that is communicated with and possesses the diameter littler with the bottom of said upper tube than upper tube; The lower tube that is communicated with and possesses the diameter bigger with the bottom of said intervalve than intervalve; In when centrifugation, in order to prevent because the distortion of the intervalve that centrifugal force causes, the more than one support chip that forms as one with the outer wall of said intervalve.
The present invention relates to a kind of outer wall that possesses the carrying current substance in the inner space; The stream material that is contained in inside is carried out the centrifugal separating tube of component separating according to its proportion; Said centrifugal separating tube possesses: opening is arranged at top, and possesses the upper tube of predetermined internal capacity; The intervalve that is communicated with and possesses the diameter littler with the bottom of said upper tube than upper tube; And can insert the conical tube of 15ml and 50ml; For through once centrifugalizing and can the nucleus layer being formed at the intermediate layer, with lower tube and intervalve, the volume of upper tube is pressed estimated rate and is formed; And, adhere to the more than one support chip of all-in-one-piece with outer wall in the outer wall formation of intervalve in order to prevent the distortion of intervalve when centrifugalizing.The bottom of this support chip extends to the bucket of whizzer.
The present invention is characterised in that upper tube has the bottle stopper that can block opening, and this bottle stopper is to adopt the material of flexibility to make, and is formed by a kind of in rubber, silicon, PVC, polyethylene, polypropylene or the polymer especially.
It is outstanding so that can carry out disinfection before inserting entry needle to the outside that the present invention is characterised in that the bottle stopper that can block the upper tube upper end is designed to central portion.And form in the upper end of upper tube and to combine projection, and further formation can be with the block that has engagement groove that combines projection to combine so that bottle stopper be difficult for separating from upper tube.
The present invention is characterised in that the thickness of its bottom surface of lower tube is thinner than the thickness of side and has flexibility, and this design is just to turn the volume of lower tube down so that small composition is positioned at intervalve in order to be necessary.
The present invention relates to a kind of outer wall that possesses the carrying current substance in the inner space, use bag according to the centrifugation that proportion separates its composition, it is characterized in that opening is arranged at top, and possess the upper tube of predetermined internal capacity being contained in inner stream material; The intervalve that is communicated with and possesses the diameter littler with the bottom of said upper tube than upper tube; The lower tube that is communicated with and possesses the diameter bigger with the bottom of said intervalve than intervalve; Form said upper tube, form hanging hole on the crust of intervalve and lower tube, and said hanging hole is hung on the dull and stereotyped bucket that possesses hook, and, the bottom inlet formed in centrifugation with the bottom of bag.
The present invention is characterised in that to possess the outer wall of carrying current substance in the inner space, and with being contained in the centrifugal separating tube of inner stream material according to its composition of proportion separation, the outer wall of its intervalve is designed to be thicker than the outer wall of upper tube and lower tube.
The present invention is characterised in that; In centrifugal separating tube, inject in advance the anti-freezing solidity materials such as fibrin ferment, calcium divalent ion, bead fibrin active matters such as (Glass beads) or heparin, ACDA, CPDA, EDTA of scheduled volume; Or separation materials such as the water-soluble high-molecular compound of precipitable particular matter or Ficoll-Hypaque (Ficoll-Hypaque), or the fluorescent marker of antigen-antibody reaction separates reagent or magnetic micro-beads (magnetic microbead).
The present invention is characterised in that, the expression portion of expression centrifugation amount is formed at the outer surface of upper tube.
The present invention is the centrifugal separating tube that possesses upper tube, intervalve and lower tube.The volume of lower tube formed account for the about 36% of all volumes of centrifugal separating tube, the volume of intervalve forms and accounts for 10 ± 2% so that the composition that will extract gathers in the intervalve.
The present invention possesses following effect.
According to the difference in specific gravity of the biology chemistry property material that will separate when centrifugalizing; Side through at centrifugal separating tube forms support chip; Ability is separated in the strong centrifugal force and the acceleration of gravity that when the centrifugal separating tube that possesses minor diameter centrifugalizes, produce, and the separation of this separation difference in specific gravity that to be the utilization that only just can be carried out by the little centrifugal separating tube of diameter accurate.For example, through utilize according to proportion and the centrifugal separating tube that the component ratio difference is carried out of the peripheral blood that will separate, the buffy coat that will contain stem cell is formed in the intervalve of minor diameter, thereby effectively accomplishes the separation of buffy coat.
Because the simple support chip that forms; Compared with existing method, can reduce the outside block of other formation or the inconvenience that intermediary brings is set, can enough cheap prices make centrifugal separating tube; And also need not to use the washing process that to remove this thing behind the artificial polymer; Just can carry out accurate centrifugation, therefore, have breadboard easy to operate effect.
The bottom thickness of the lower tube of centrifugal separating tube forms slim slightly; Utilize its flexibility; Can the solution that be formed at lower tube not shifted onto in the intervalve even do not form other screw or plug yet, therefore, can with the material that will separate be arranged in the intervalve of narrower.
Centrifugation is made the sack shape with container, even do not move blood to other containers, also carries out the separation of blood material easily; If when the material of wanting was formed at lower tube, the simple press lower tube made the material that is arranged in lower tube move to intervalve; Then carry out the separation of material again, accomplish in the bags of blood of 1 centrifugation, the intervalve of in this bags of blood, distinguishing by red blood cell and other compositions; Can be disconnected from each other through tweezers or welding machine (welding machine), therefore need not use other container to take care of, and when being necessary; On the red blood cell direction, also form and to extract the bottle stopper of blood, so that can conveniently separate.
Be injected in the centrifugal separating tube biology chemistry material or the material of accomplishing centrifugation will be will carry out separation and Extraction the time; Through forming the bottle stopper that can penetrate needle tubing; Thereby have the biological contamination that flows in the time of to stop the uncork plug, realize the advantage of the centrifugation operation of safety.
With the centrifugal separating tube of minor diameter when whizzer takes out because support chip can prevent falling down of centrifugal separating tube, therefore, even said support chip can help to realize under the erectility of ground, also centrifugalizing the lock out operation of material.
Owing to put on according to the male sex on the surface of centrifugal separating tube or the women does not index line or indication literal, the signifying sign etc. of isolabeling with the blood flow volume that injects; Therefore; Can help the operator as long as be injected into easily till the index line, also can the required buffy coat that contains stem cell be separated to the intervalve part.
Through 1 centrifugation, just can in blood, extract the buffy coat of the composition of wanting easily in the whizzer of the 15ml conical tube that the most often uses in present various medical office or the laboratory.
Centrifugal separating tube of the present invention; The volume that is about to lower tube form centrifugal separating tube whole volumes about 36%; The volume of intervalve accounts for 10 ± 2%; If use said centrifugal separating tube to carry out the centrifugation of blood, the buffy coat that institute will extract can accumulate in intervalve, therefore extraction easily.
Description of drawings
Fig. 1 is the exemplary plot that existing centrifugal separating tube is shown.
Fig. 2 is the exemplary plot that existing blood taking bag is shown.
Fig. 3 is the illustration sectional view that existing centrifugal separating tube is shown.
Fig. 4 is the stereogram that an embodiment of centrifugal separating tube of the present invention is shown.
Fig. 5 is the stereogram that an embodiment of centrifugal separating tube of the present invention is shown.
Fig. 6 be illustrate Fig. 4 along A-A line sectional view.
Fig. 7 be illustrate Fig. 5 along C-C line sectional view.
Fig. 8 be illustrate Fig. 4 along B-B line sectional view.
Fig. 9 be illustrate Fig. 5 along D-D line sectional view.
Figure 10 is the sectional view that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 11 is the sectional view that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 12 is the drawing that illustrates for the embodiment that blood taking bag of the present invention is described.
Figure 13 is the drawing that illustrates for the embodiment that blood taking bag of the present invention is described.
Figure 14 is the drawing that illustrates for the embodiment that blood taking bag of the present invention is described.
Figure 15 is the drawing that illustrates for the embodiment that blood taking bag of the present invention is described.
Figure 16 is the stereogram that dull and stereotyped bucket of the present invention is shown.
Figure 17 is the stereogram that centrifugal separating tube of the present invention is shown.
Figure 18 is the stereogram that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 19 be illustrate Figure 17 along A-A line sectional view.
Figure 20 be illustrate Figure 18 along C-C line sectional view.
Figure 21 be illustrate Figure 17 along B-B line sectional view.
Figure 22 be illustrate Figure 17 along D-D line sectional view.
Figure 23 is the stereogram that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 24 is the exploded perspective view that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 25 is the front elevation that an embodiment of centrifugal separating tube of the present invention is shown.
Figure 26 be illustrate Figure 25 along A-A ' line sectional view.
The specific embodiment
According to Fig. 4 to 16 explanation embodiments of the invention.
Fig. 4 is the figure that an embodiment of centrifugal separating tube of the present invention is shown; Said centrifugal separating tube possesses lower tube 50 draws that the centrifugation back supplies the heaviest material aggregation of proportion; Intervalve 40 draws of the material aggregation in the middle of confession proportion belongs to; Supply upper tube 30 draws of the lightest material aggregation of proportion, support said intervalve on the next door of intervalve and be connected in upper tube and the support chip of lower tube 80.Can put on the scale that to confirm the liquor capacity capacity on this support chip 80.Support chip 80 forms more than one, preferably forms by three, and this type of support chip 80 not only is formed on the side of the intervalve 40 of centrifugal separating tube, can also be formed on the side of upper tube 30 or lower tube 50.And, can put on the scale of confirming the liquor capacity capacity on this support chip 80.And; Form the rubber of penetrable needle tubing or the bottle stopper 90 of chemical polymerization thing materials such as silicon, polyethylene, polypropylene or PVC on the top of centrifugal separating tube, said bottle stopper 90 can be selected plug-in type or form screw thread and form the mode that screw inserts with rotation with it symmetrically at centrifugal separating tube.
An example possesses following numerical value among the concrete embodiment of centrifugal separating tube of the present invention.The diameter of the upper tube 30 of centrifugal separating tube, intervalve 40, lower tube 50 and length form by predetermined ratio; Be applicable in the whizzer of the centrifugal separating tube that all insert existing 15ml and 50ml; Need not other devices and just easily the buffy coat of required composition in the blood is formed in the intervalve 40, also extract said buffy coat easily.Promptly; The external diameter of centrifugal separating tube is when being applicable to the centrifugation adapter that 15ml uses; Upper tube 30 is kept 16mm ± 2mm, and the external diameter of lower tube 50 is also kept 16mm ± 2mm, and the external diameter of intervalve 40 is kept 6mm ± 2mm; The volume of lower tube 50 forms 36% of the volume that is no more than all centrifugal separating tubes, the volume of intervalve 40 form all centrifugal separating tubes volume 10% ± 2%.When perhaps being applicable to the centrifugal separating tube of 50ml centrifugation bucket; The external diameter of upper tube 30 is kept 28mm ± 2mm; The external diameter of lower tube 50 is kept 28mm ± 2mm; The external diameter of intervalve 40 is kept 6mm ± 2mm, and the volume of lower tube 50 forms 36% of the volume that is no more than all centrifugal separating tubes, the volume of intervalve 40 form all centrifugal separating tubes volume 10% ± 2%.Whether irrelevant the formation of said centrifugal separating tube and support chip 80 is, all the time buffy coat is formed in the intervalve 40 during centrifugation blood.
When using this type of centrifugal separating tube to extract centrifugation such as blood; To gather lower tube 50 than heavy red blood cell; The buffy coat of leucocyte and blood platelet etc. then is separated to intervalve 40, and the strong gravity acceleration g that support chip 80 produces when preventing intervalve 40 because of centrifugation deforms.And, can biological fluids such as blood be injected in the centrifugal separating tube even do not open bottle stopper 90 yet, therefore can prevent sneaking into of virus or bacterium.Because the medical apparatus or the biology machine of strict sterilization can carry out hands-off operation, can prevent to check therefore that body or culture or centrifugation thing are contaminated.
Fig. 5 is another embodiment that centrifugal separating tube of the present invention is shown, and removes the lower tube of Fig. 4, and intervalve 40 extends to the form of the bottom of centrifugal separating tube, can select to use according to the characteristic of biological solns.That is, be the centrifugal separating tube of making in order to be applicable to the centrifugation similar small volume of solution of whole proportion.Said centrifugal separating tube can use when the more accurate centrifugation of needs, for example, if use when red blood cell is similar with blood platelet and leukocytic composition in 1 separate blood, just more precision and easy separation.
The centrifugal separating tube of this type of form; Through extending to the bottom and being formed on the support chip 80 of the side of intervalve 40; In when centrifugation,, also can prevent the distortion of intervalve 40 or rock even be added under the pressure of strong gravity acceleration g of intervalve 40; The solution that holds can fitly be arranged according to the difference in specific gravity of each composition, thereby can carry out more accurate centrifugation.Moreover, when on the very narrow intervalve 40 of diameter, being difficult to the volume of label solution, can on support chip 80, fully engrave the scale of this capacity.And can form laterally barrel can the combining closely of end and whizzer that the alar part that prolongs makes support chip 80, and be put on the average floor after can from whizzer, taking out, make things convenient for lock out operation.
Fig. 6 be illustrate Fig. 4 along A-A line sectional view; Fig. 7 be illustrate Fig. 5 along C-C line sectional view, the section morphology of upper tube 30 and lower tube 50 is pentagon, but might not want pentagon; Can as required section morphology be made into del or triangle or quadrangle.And; The thickness of the bottom surface of said lower tube 50 forms the thickness that is thinner than wall and has flexibility, if when buffy coat accumulates in said lower tube 50, pushes away the soft of the bottom surface of lower tube 50; Make buffy coat move on to intervalve 40, thus easy separation.Like Fig. 5 and shown in Figure 7, be formed at the bottom of support chip 80 of the side of intervalve 40, its tail end prolongs can make the said support chip 80 and the bucket of centrifugal separating tube combine closely, and during centrifugation, plays the effect that prevents that centrifugal separating tube from rocking.
Fig. 8 and Fig. 9 illustrate respectively along B-B line sectional view and along D-D line sectional view.Surface at the intervalve 40 of minor diameter forms more than one support chip 80.
Figure 10 be illustrate to carry out more accurate separation component distributing middle when above, separation for ease constitutes the intervalve 40 of minor diameter up, Figure 13 be select in a spot of on the whole solution to separate material the time, be easy to use.Said various situation, support chip 80 not only prevents the distortion of centrifugal separating tube since the alar part of the prolongation of support chip 80 combine closely in centrifugation with bucket on, the scattering of limiting bed that also can prevent to rock each composition that causes.
Figure 12, Figure 13, Figure 14 illustrate one of blood taking bag of the present invention to implement illustration, and the blood taking bag 20 that uses when taking care of behind the extraction blood uses the PVC material more.Blood taking bag 20 is distinguished into upper tube 31, intervalve 41, lower tube 51, and the formation thing in the blood of centrifugation back then separates according to each proportion.At this moment; Take place when the support chip 81 that is formed at the mid portion of blood taking bag 20 can stop centrifugation because the tearing or be out of shape of the intervalve 41 of narrower that strong gravity acceleration g causes and microscler attitude; Therefore the fixedly effect of the shape of intervalve 41 in the time of also can playing each composition of separation and Extraction can prevent rocking or scattering of buffy coat.
Figure 16 illustrates the epitrochanterian dull and stereotyped bucket 120 that is hung on whizzer, and blood taking bag 20 is hung on the hook 110 that is formed on the said dull and stereotyped bucket 120.At this moment, be formed at the hook 110 of the hanging hole 100 corresponding dull and stereotyped buckets 120 on the blood taking bag 20.
And, assemble the nucleus layer of blood platelet or leukocytic cream in the blood of centrifugation if when being formed on lower tube 51, gently press the downside of lower tube 51; Said buffy coat just can move on to upside a little makes buffy coat be formed at intervalve 41, therefore, is easy to utilize needle tubing or capillary to separate; Be the intervalve 41 of minor diameter because blood plasma, red blood cell and blood platelet be respectively formed at narrow width, therefore again; Its interface can use long forceps or forcing press (pressing machine), and welding machine waits gripping and separates, and connects if utilize welding machine to wait; The blood constituent that just will centrifugalize need not move on in each other container and can store yet, and, shown in figure 15; Through bottom inlet 92, can extract red blood cell in addition out.
In the centrifugal separating tube, can add the separations material such as water-soluble high-molecular compound or Ficoll-Hypaque of deposition particular matter or also can add anti-freezing solidity materials such as utilizing heparin, ACDA, CPDA, EDTA earlier or the fluorescent marker separation reagent of fibrin active matter such as fibrin ferment, calcium divalent ion, bead or antigen-antibody reaction or magnetic micro-beads etc.The symbol of record on drawing symbology Fig. 1-16 of said explanation.
Figure 17 is another embodiment that centrifugal separating tube of the present invention is shown; When extraction blood etc. centrifugalizes; To lower tube 50, the buffy coat of leucocyte and blood platelet etc. then is separated in intervalve 40 than heavy erythrocyte aggregation, and the strong gravity acceleration g that takes place when centrifugalizing in order to stop makes intervalve 40 distortion; The thickness of the intervalve 40 of minor diameter is formed upper tube 30 or the lower tube 50 that is thicker than other wide diameters, thereby also ability is under strong gravity acceleration g.And the inlet position of upper tube 30 forms the thick slightly type bottle stopper 20 of can combining closely.Form the bottle stopper of being processed by chemical polymerization thing materials such as rubber or silicones, polyethylene, polypropylene or PVC 20 of penetrable needle tubing on the top of centrifugal separating tube, said bottle stopper 20 can be selected plug-in type or on centrifugal separating tube, form screw thread and form the mode that screw inserts with rotation with it symmetrically.Promptly; During the biological fluid of centrifugation blood etc.; Even not opening or closing bottle stopper 20 also can be injected into liquid such as blood in the centrifugal separating tube; Sneaking into of anti-virus or bacterium can freely centrifugalize, because the medical apparatus or the biology machine of strict sterilization can carry out hands-off operation, therefore can prevent to check body or culture or the pollution of centrifugation thing.
Figure 18 is another embodiment that centrifugal separating tube of the present invention is shown; Remove lower tube; Intervalve 40 is made into the form of the bottom that extends to centrifugal separating tube, can selects to use, also be applicable to the centrifugation of the small volume of solution that whole proportion is similar according to the characteristic of biological solns.Said centrifugal separating tube can use when needs more critically centrifugalize, for example, if use when red blood cell is similar with blood platelet and leukocytic composition in 1 separate blood, just can more accurate and convenient separation.Be formed on the support chip 80 of bottom, prolong laterally in order combining closely, and to be put on the average floor after can from whizzer, taking out, make things convenient for centrifugally operated with the bucket of whizzer.
Figure 19 and Figure 20 illustrate respectively along A-A line sectional view and along C-C line sectional view, and the cross sectional shape of upper tube 30 and lower tube 50 is not necessarily wanted pentagon, can as required cross sectional shape be made into del or triangle or quadrangle.And the thickness of said lower tube 50 forms the thickness that is thinner than wall and has flexibility, if buffy coat accumulates in said lower tube 50, push lower tube 50 and make buffy coat move on to intervalve 40, thus easy separation.Like Figure 18 and shown in Figure 20, be formed at the support chip 80 of the downside of intervalve 40, said support chip 80 forms with the inner tight of the bucket of whizzer and combines, when centrifugalizing, plays the effect that prevents that centrifugal separating tube from rocking.
Figure 21 and Figure 22 illustrate respectively along B-B line sectional view and along D-D line sectional view.The thickness of intervalve 40 forms the thickness that is thicker than upper tube 30; Also can prevent distortion even produce strong gravity acceleration g when making centrifugation; And assemble the nucleus layer of blood platelet or leukocytic cream in the blood of centrifugation if when being formed on lower tube 50, and gently press the downside of lower tube 50, said buffy coat just can move on to upside a little makes buffy coat be formed at intervalve 40; Therefore, be easy to utilize needle tubing or capillary to separate.The symbol of record on the drawing symbology Figure 17-22 that uses in the said explanation.
Embodiment
Figure 23 is the stereogram that an embodiment of centrifugal separating tube of the present invention is shown; When extraction blood etc. centrifugalizes; Red blood cell layer than heavy gathers lower tube 50; The buffy coat that contains the stem cell of leucocyte and blood platelet etc. then is separated in intervalve 40, and plasma fractions such as serum are formed at upper tube 30.
The index line 100,101 or the indication literal that on the surface of the predetermined altitude of upper tube 30, possess the amount of the blood that drops in the indication centrifugal separating tube, expression portions such as signifying sign.At this moment, the periphery blood volume that drops into during index line 100 expression women, and the periphery blood volume that drops into during the index line 101 expression male sex.Said centrifugal separating tube makes the red blood cell component that is gathered in lower tube 50 than similar through dropping into different amounts, therefore need not to make centrifugal separating tube respectively according to the men and women, and common the use.That is, poor according to men and women's blood constituent, in centrifugal separating tube, isolate erythrocytic lower tube 50 and can form predetermined volume.Said index line 100,101 is not only marked one respectively, and shown in the intervalve of Fig. 4 and Fig. 5, available gauge table timberline forms subscale timberline (expression scale).At this moment, measurable and observe the volume of each composition, therefore use more convenient.Said expression line is illustrated in intervalve, but also can be illustrated in upper tube and/or lower tube.On this expression line, put on men and women's mark, also can use as index line simultaneously.Existing centrifugal separating tube, as shown in Figure 3, form other plug 60 in lower tube 50; Adjust the volume of lower tube 50 through the mode of tightening or turning on, this mode can cause the increase of manufacturing cost, again; Some is in order to reduce expenses; Just re-use after washing of breakaway plug 60 back and the sterilization, this method that re-uses the non-health of the plug 60 of not removing the blood that has other people on the screw etc. fully can cause using danger, and present embodiment can solve said problem.
The ratio of the red blood cell component of masculinity and femininity is 47% and 42%; The amount of the anticoagulant that drops in the leaching process of peripheral blood; Usually insignificant during heparin; But consider the ratio of about 10-15% that will add blood during the anticoagulant of ACDA etc., therefore, form the index line 100,101 of indication periphery blood volume on the surface of upper tube 30.And; For the amount of accomplishing to drop into according to men and women or the amount of the anticoagulant of interpolation different; The thick line of these type of index line 100,101 usefulness comes mark, and this type of index line 100,101 has shown as thickness, even therefore same index line 100,101; The upside indication anticoagulant input amount of line is few, and the downside of line indication anticoagulant input amount is many.Though do not mark at drawing, mark by color or mark, literal, therefore when lock out operation, can the nucleus layer that comprise required stem cell be positioned at certain part of intervalve 40, thus separate stem cells effectively.The scale of said index line 100,101 and all volumes of expression can be done other mark, also does same mark with the scale of all volumes of expression.When the material of putting into centrifugal separating tube was not the mankind's peripheral blood, index line 100,101 can be done not isolabeling according to the difference of separate substance.
And; Shown in figure 24; The bottle stopper of being processed by chemical polymerization thing materials such as rubber or silicon, polyethylene, polypropylene or PVC 20 of penetrable needle tubing is formed at the top of said centrifugal separating tube; Said bottle stopper 20 can be selected plug-in type or also form the mode that screw inserts with rotation at centrifugal separating tube formation screw thread with it symmetrically, and the form of projection is slightly formed at the top of bottle stopper 20, when making the preceding use of insertion needle tubing alcohol swab wait wiping; The cap 90 of need not uncapping also is prone to wiping, and its inside then forms recessed form.This is can accumulate in recessed position during for the particular matter in collecting centrifugal separating tube.
And, except that bottle stopper 20, form the open block 90 in top, the phenomenon that bottle stopper 20 was opened when the block 90 that is made up of the open structure in top can prevent to extract the needle tubing that is inserted into bottle stopper 20 again.Block 90 face formation within it engagement groove 81 is engaged in the corresponding with it combination projection 80 in centrifugal separating tube.Through as above forming; Even do not open or close bottle stopper 20 or block a shot 90; Also can peripheral blood be injected in the centrifugal separating tube or the material of can separation and Extraction required composition; Therefore the effect of sneaking into that possesses virus of preventing or bacterium, therefore the medical apparatus of strict sterilization or biology machine can prevent to check body or culture or the pollution of centrifugation thing owing to can carry out hands-off operation.Said combination projection 80 and engagement groove 81 can be formed by screw thread etc.The thickness of said lower tube 50 forms the thickness that is thinner than other two pipes and flexibility is arranged slightly, if when buffy coat accumulates in said lower tube 50, the soft position of pushing lower tube 50 makes buffy coat move on to intervalve 40, thus easy separation.Though show, can form support chip on the surface of the intervalve 40 of narrower at drawing.
When the method that the needle tubing that inserts syringe extracts buffy coat is injection the afterbody of needle tubing is inserted into can reach to extract behind the buffy coat and gets final product.The afterbody of buffy coat and the red blood cell of bottom form boundary through thin layer; Use this moment the afterbody of needle tubing in the wall of intervalve, draw a circle like revolution; Through centrifugal force, heavy red blood cell is attached to the wall of intervalve, in the middle of relative light buffy coat then gathers; This moment, the remaining trace of buffy coat also can extract if use the method that sucks the buffy coat that accumulates in central authorities through the afterbody of needle tubing.
Shown in figure 25, the surface of centrifugal separating tube forms the presentation surface 110 of the name that can write down those who are investigated or check day etc.Through forming said presentation surface 110, when doing a lot of inspection simultaneously, the examiner can prevent the mistaken diagnosis that the chaos owing to those who are investigated causes.
Figure 26 be illustrate Figure 25 along A-A ' line sectional view, form on the surface of centrifugal separating tube and to combine projection 80 to be engaged in the engagement groove 81 that the medial surface of the block 90 of corresponding position with it forms.The symbol of record on the drawing symbology Figure 23-26 that uses in the said explanation.
In the centrifugal separating tube of the present invention; Water-soluble high-molecular compound or the Ficoll-Hypaque etc. that can add precipitable particular matter separate material; Or the fluorescent marker that injects fibrin active matter such as anti-freezing solidity materials such as utilizing heparin, ACDA, CPDA, EDTA or fibrin ferment, calcium divalent ion, bead or antigen-antibody reaction in advance separates reagent or magnetic micro-beads etc.; Purposes through required is easy to use.
Various embodiments has been described as stated, in addition, has been expected easily carrying out more various embodiment that scope of the present invention should comprise these embodiment based on technological thought of the present invention.
The present invention separates centrifugal blood, isolates special composition, utilizes the composition that separates to use in the medical biotechnology engineering again.Utensil of the present invention when separation, cultivation and regenerative cell, can cut off take place in the separation process contain biology in interior pollution etc., and economy safety again helps effectively separating bio and learns material.
Claims (22)
1. centrifugal separating tube possesses the outer wall of carrying current substance in the inner space, carry out component separating with being contained in inner stream material according to proportion; Said centrifugal separating tube is characterised in that; Comprise: upper tube, top has inlet, and possesses predetermined internal capacity; Intervalve, it is communicated with the bottom of said upper tube, and possesses the diameter littler than upper tube; Lower tube, it is communicated with the bottom of said intervalve, and possesses the diameter bigger than intervalve; More than one support chip, it is outside integrally formed said intervalve.
2. centrifugal separating tube possesses the outer wall of carrying current substance in the inner space, carry out component separating with being contained in inner stream material according to proportion; Said centrifugal separating tube is characterised in that; Comprise and containing: upper tube, top has inlet, and possesses predetermined internal capacity; Intervalve, it is communicated with the bottom of said upper tube, and possesses the diameter littler than upper tube; More than one support chip, it is outside integrally formed said intervalve.
3. centrifugal separating tube according to claim 1 and 2 is characterized in that said centrifugal separating tube further comprises bottle stopper; Bottle stopper adopts flexible material to process, and clogs the inlet of upper tube.
4. centrifugal separating tube according to claim 3 is characterized in that, said bottle stopper forms by one in rubber, silicon, PVC, polyethylene, polypropylene or the polymer.
5. centrifugal separating tube according to claim 2 is characterized in that, the bottom of said support chip extends to the bucket of whizzer.
6. centrifugal separating tube according to claim 1 is characterized in that, the thickness of the bottom surface of said lower tube is thinner than the thickness of side, and the bottom surface of lower tube has pliability.
7. centrifugal separating tube; The crust that possesses the carrying current substance in the inner space carries out component separating with being contained in inner stream material according to proportion, and said centrifugation pipeline is characterised in that and comprises: upper tube; Top has inlet, and possesses predetermined internal capacity; Intervalve, it is communicated with the bottom of said upper tube, and possesses the diameter littler than upper tube; Lower tube, it is communicated with the bottom of said intervalve, and possesses the diameter bigger than intervalve; On the crust that forms said upper tube, intervalve and lower tube, form hanging hole, said hanging hole is hung on the dull and stereotyped bucket that possesses hook.
8. centrifugation according to claim 7 is characterized in that with bag, forms the bottom inlet in the bottom of centrifugation bag.
9. centrifugal separating tube; The outer wall that possesses the carrying current substance in the inner space carries out component separating with being contained in inner stream material according to proportion, and said centrifugal separating tube is characterised in that and comprises: upper tube; Top has inlet, and possesses predetermined internal capacity; Intervalve, it is communicated with the bottom of said upper tube, and possesses the diameter littler than upper tube; Lower tube, it is communicated with the bottom of said intervalve, and possesses the diameter bigger than intervalve; The outer wall of said intervalve is than the outer wall thickness of upper tube and lower tube.
10. centrifugal separating tube; The outer wall that possesses the carrying current substance in the inner space carries out component separating with being contained in inner stream material according to proportion, and said centrifugal separating tube is characterised in that and comprises: intervalve; Opening is arranged at its top, and possesses predetermined internal capacity; Lower tube, it is communicated with the bottom of said intervalve, and possesses the diameter bigger than intervalve; The outer wall of said intervalve is than the outer wall thickness of lower tube.
11., it is characterized in that the bottle stopper of centrifugal separating tube is processed by rubber, silicon, PVC, polyethylene, polypropylene or polymer plastic or its combination according to claim 9 or 10 described centrifugal separating tubes.
12. according to claim 9 or 10 described centrifugal separating tubes, it is characterized in that, form more than one support chip in the side of intervalve; Or forming more than one support chip in the side of intervalve, support chip extends to the bucket of whizzer.
13. according to each described centrifugal separating tube in the claim 1 to 12; It is characterized in that, can inject fibrin active matters such as fibrin ferment, calcium divalent ion, bead in the pipe, or anti-freezing solidity materials such as heparin, ACDA, CPDA, EDTA; Or the water-soluble high-molecular compound of precipitable particular matter; Or Ficoll-Hypaque etc. separates material, or utilizes the fluorescent marker of antigen-antibody reaction to separate reagent, or magnetic micro-beads.
14. centrifugal separating tube; Big relatively lower tube and the upper tube of intervalve, diameter that comprises minor diameter; It is characterized in that, form the expression portion of component of amount and the composition of the object that expression needs to centrifugalize at the outer surface of intervalve, upper tube or lower tube.
15. a centrifugal separating tube comprises the big relatively lower tube and the upper tube of intervalve, diameter of minor diameter, it is characterized in that, uses the bottle stopper of middle body projection to clog the upper end of upper tube.
16. centrifugal separating tube according to claim 15 is characterized in that, forms in the upper end of upper tube to combine projection; Described centrifugal separating tube further provides block, and block has the engagement groove that can combine with said combination projection, makes said bottle stopper not separate from upper tube easily.
17. centrifugal separating tube according to claim 15 is characterized in that, the material of the bottle stopper of upper tube is rubber or silicon, and the bottle stopper of upper tube forms exterior protrusions and the inner recess of middle body.
18. according to claim 14 or 15 described centrifugal separating tubes, it is characterized in that the thickness of the outer wall of said lower tube is thinner than the outer wall of upper tube and intervalve, the outer wall of said lower tube has pliability.
19. according to each described centrifugal separating tube in the claim 14 to 18; It is characterized in that, inject anti-freezing solidity materials such as heparin, ACDA, CPDA, EDTA in advance in pipe inside, or the water-soluble high-molecular compound of precipitable particular matter; Or Ficoll-Hypaque etc. separates material; Or fibrin active matters such as fibrin ferment, calcium divalent ion, bead, or utilize the fluorescent marker of antigen-antibody reaction to separate reagent, or magnetic micro-beads.
20. a centrifugal separating tube possesses upper tube and intervalve and lower tube, it is characterized in that, the volume of lower tube is formed centrifugal separating tube whole volumes about 36%; The volume of intervalve is formed 10 ± 2% of whole volumes, make the composition that will extract to gather intervalve.
21. centrifugal separating tube according to claim 20 is characterized in that, when said centrifugal separating tube was used on the centrifugation adapter of 15ml type, the upper tube external diameter was 16 ± 2mm; The external diameter of lower tube is 16 ± 2mm; The external diameter of intervalve is 6 ± 2mm.
22. according to claim 20, said centrifugal separating tube is characterized in that, when said centrifugal separating tube was used on the centrifugation adapter of 55ml type, the external diameter shape of upper tube was 28 ± 2mm; The external diameter of lower tube is 28 ± 2mm; The external diameter of intervalve is 6 ± 2mm.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20090127565 | 2009-12-19 | ||
| KR10-2009-0127565 | 2009-12-19 | ||
| KR10-2010-0004358 | 2010-01-18 | ||
| KR20100004358 | 2010-01-18 | ||
| PCT/KR2010/008968 WO2011071353A2 (en) | 2009-12-07 | 2010-12-15 | Centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102844121A true CN102844121A (en) | 2012-12-26 |
Family
ID=47370871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010800554604A Pending CN102844121A (en) | 2009-12-19 | 2010-12-15 | Centrifuge tube |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2013514874A (en) |
| CN (1) | CN102844121A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105368706A (en) * | 2015-12-11 | 2016-03-02 | 何守贵 | High-volume mononuclear cell separating bottle |
| CN106361354A (en) * | 2016-08-31 | 2017-02-01 | 秦明 | Blood sampling test-tube |
| CN106582909A (en) * | 2016-12-30 | 2017-04-26 | 张方亮 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
| CN111544924A (en) * | 2020-05-14 | 2020-08-18 | 中国水利水电科学研究院 | Liquid-liquid extraction separation quantitative pipe |
| WO2021093775A1 (en) * | 2019-11-14 | 2021-05-20 | 北京酷搏科技有限公司 | Reaction tube, reaction tube array, and method for controlling volume of sample participating in reaction and application thereof |
| CN114452684A (en) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation kit and extraction method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7170042B2 (en) * | 2017-11-30 | 2022-11-11 | ヤンタイ・アウスビオ・ラボラトリーズ・カンパニー・リミテッド | Methods, systems and filtration units for isolating particles from biological samples |
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| CN114452684A (en) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation kit and extraction method |
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|---|---|
| JP2013514874A (en) | 2013-05-02 |
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Application publication date: 20121226 |