CN102836194A - Extraction method of affine cudweed total flavonoids - Google Patents
Extraction method of affine cudweed total flavonoids Download PDFInfo
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- CN102836194A CN102836194A CN2011101776228A CN201110177622A CN102836194A CN 102836194 A CN102836194 A CN 102836194A CN 2011101776228 A CN2011101776228 A CN 2011101776228A CN 201110177622 A CN201110177622 A CN 201110177622A CN 102836194 A CN102836194 A CN 102836194A
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- herba gnaphalii
- gnaphalii affinis
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Abstract
The invention discloses an extraction method of affine cudweed total flavonoids, and belongs to the field of medicinal material processing. The extraction method comprises the following steps of: crushing and screening dried affine cudweed; extracting with cellulose by using water as an extracting agent; filtering, and concentrating in reduced pressure, and lyophilizing to obtain the affine cudweed total flavonoid product. The extraction method has the advantages of easy and quick operation, low cost and no toxicity, and is a green production method.
Description
Technical field
The present invention relates to a kind of method of from Herba Gnaphalii Affinis, extracting total flavones, specifically is a kind of method of utilizing cellulase to extract the Herba Gnaphalii Affinis total flavones, belongs to field of medicinal materials processing.
Background technology
Herba Gnaphalii Affinis (Gnaphlium affine D.Don) has another name called Herba Gnaphalii Affinis, affine cudweed, is the Compositae cottonweed, has multiple efficacies such as blood fat reducing, blood sugar lowering, blood pressure lowering, defying age, antiinflammatory are antibacterial, enhancing immunity.Before and after the Ching Ming Festival, the tender sharp leaf processing bait of Herba Gnaphalii Affinis that extensively utilizes among the people is so there is the title of " affine cudweed ".There are some researches show, contain more flavone compound in the Herba Gnaphalii Affinis.But; The domestic and international at present research to the Herba Gnaphalii Affinis flavone compound also quite lacks; And flavone compound has remarkable result to treatment coronary heart disease, alzheimer disease, cerebral thrombosis, nervous system disease and elimination free radical, aspect such as antibacterial, anticancer; Have no side effect, and develop multiple medicine and health food with this.Therefore, how efficiently and safely the Herba Gnaphalii Affinis flavone compound to be extracted, also more and more receive the attention of Chinese scholars.
Total flavone extracting method commonly used has the decocting cooking method, and hot reflux is extracted, ultrasonic-assisted extraction, microwave-assisted extraction etc.But decocting cooking method extraction time is very long, general 4～6 hours; Hot reflux is extracted, and ultrasonic-assisted extraction and microwave-assisted extract total flavones then to be needed with an organic solvent; And Enzymatic Extraction is as a kind of good method for distilling; Have simple and efficient to handle, extraction time is short, the rate of transform is high and characteristics such as solvent-free pollution; Oneself is widely used in the extraction aspect of bioactive substance at present, like Folium Crataegi total flavones, and Folium Ginkgo total flavones; Herba Apii graveolentis flavone etc. do not appear in the newspapers and be applied to Herba Gnaphalii Affinis total flavones extraction aspect so far.
Summary of the invention
The method for distilling that the purpose of this invention is to provide a kind of Herba Gnaphalii Affinis total flavones, promptly a kind of method of utilizing cellulase to extract the Herba Gnaphalii Affinis total flavones.
For realizing above-mentioned purpose, the present invention adopts following technical scheme: exsiccant Herba Gnaphalii Affinis was pulverized 40 mesh sieves, and water is extractant; The cellulase consumption is 0.3%～0.5% of a Herba Gnaphalii Affinis raw materials quality, the ratio 10ml/g～14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.0～5.0; 40 ℃～50 ℃ of hydrolysis temperatures; Enzymolysis time 50min～70min gets extracting solution, through checking the total flavones rate of transform greater than 90%; Extracting solution is adopted the middling speed filter paper filtering under vacuum 0.08MPa～0.10MPa and 5 ℃～35 ℃ conditions of temperature, get filtrate; With filtrate be concentrated under vacuum 0.08MPa～0.10MPa and 50 ℃～60 ℃ conditions of temperature initial volume 30% till, concentrated solution; With concentrated solution freezer dryer vacuum less than 20Pa, condenser temperature-45 ℃～-60 ℃ conditions under drying totally, Herba Gnaphalii Affinis total flavones product, through check purity greater than 3.5%.
Owing to adopted above technical scheme, the present invention has the following advantages:
1, the present invention utilizes cellulase to extract the Herba Gnaphalii Affinis total flavones; Its major advantage is to be extractant with water; The employing cellulase extracts; Compare with alcohol reflux, the auxiliary ethanol extraction of ultrasound wave or microwave-assisted ethanol extraction, do not use organic solvents such as ethanol during extraction, so the present invention is easy and simple to handle, quick, cheap, nontoxic;
2, extract the Herba Gnaphalii Affinis total flavones with the inventive method, its total flavones rate of transform is greater than 90%, and Herba Gnaphalii Affinis total flavones product purity is greater than 3.5%, and favorable reproducibility.
The specific embodiment
Embodiment 1
Exsiccant Herba Gnaphalii Affinis was pulverized 40 mesh sieves; The cellulase consumption is 0.40% of a Herba Gnaphalii Affinis raw materials quality, the ratio 12ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.5; 45 ℃ of hydrolysis temperatures and enzymolysis time 60min, this moment, the total flavones rate of transform was 95.38%; Extracting solution is adopted the middling speed filter paper filtering under vacuum 0.09MPa and 25 ℃ of conditions of temperature, get filtrate; With filtrate be concentrated under vacuum 0.10MPa and 60 ℃ of conditions of temperature initial volume 30% till, concentrated solution; With concentrated solution freezer dryer vacuum less than 20Pa, condenser temperature-50 ℃ condition under drying totally, Herba Gnaphalii Affinis total flavones product, this product purity is 3.99%.
Embodiment 2
Exsiccant Herba Gnaphalii Affinis was pulverized 40 mesh sieves; The cellulase consumption is 0.46% of a Herba Gnaphalii Affinis raw materials quality, the ratio 12ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.5; 47 ℃ of hydrolysis temperatures and enzymolysis time 60min, this moment, the total flavones rate of transform was 96.92%; Extracting solution is adopted the middling speed filter paper filtering under vacuum 0.09MPa and 25 ℃ of conditions of temperature, get filtrate; With filtrate be concentrated under vacuum 0.10MPa and 60 ℃ of conditions of temperature initial volume 30% till, concentrated solution; With concentrated solution freezer dryer vacuum less than 20Pa, condenser temperature-50 ℃ condition under drying totally, Herba Gnaphalii Affinis total flavones product, this product purity is 3.86%.
Embodiment 3
Exsiccant Herba Gnaphalii Affinis was pulverized 40 mesh sieves; The cellulase consumption is 0.50% of a Herba Gnaphalii Affinis raw materials quality, the ratio 14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.8; 44 ℃ of hydrolysis temperatures and enzymolysis time 50min, this moment, the total flavones rate of transform was 91.54%; Extracting solution is adopted the middling speed filter paper filtering under vacuum 0.09MPa and 25 ℃ of conditions of temperature, get filtrate; With filtrate be concentrated under vacuum 0.10MPa and 60 ℃ of conditions of temperature initial volume 30% till, concentrated solution; With concentrated solution freezer dryer vacuum less than 20Pa, condenser temperature-50 ℃ condition under drying totally, Herba Gnaphalii Affinis total flavones product, this product purity is 3.78%.
Claims (6)
1. the method for distilling of a Herba Gnaphalii Affinis total flavones is characterized in that with the Herba Gnaphalii Affinis being raw material, and exsiccant Herba Gnaphalii Affinis was pulverized 40 mesh sieves; With water is extractant, adopts cellulase to extract, and the cellulase consumption is 0.3%～0.5% of a Herba Gnaphalii Affinis raw materials quality; Ratio 10ml/g～the 14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.0～5.0,40 ℃～50 ℃ of hydrolysis temperatures; Enzymolysis time 50min～70min gets extracting solution; Through filtering, concentrating under reduced pressure and lyophilization obtain Herba Gnaphalii Affinis total flavones product again.
2. the method for distilling of a kind of Herba Gnaphalii Affinis total flavones according to claim 1 is characterized in that: the total flavones rate of transform is greater than 90% in the said extracting solution.
3. the method for distilling of a kind of Herba Gnaphalii Affinis total flavones according to claim 1 is characterized in that: said filtering method is that extracting solution is adopted the middling speed filter paper filtering under vacuum 0.08MPa～0.10MPa and 5 ℃～35 ℃ conditions of temperature, gets filtrate.
4. the method for distilling of a kind of Herba Gnaphalii Affinis total flavones according to claim 1; It is characterized in that: said concentrating under reduced pressure be with filtrate be concentrated under vacuum 0.08MPa～0.10MPa and 50 ℃～60 ℃ conditions of temperature initial volume 30% till, concentrated solution.
5. the method for distilling of a kind of Herba Gnaphalii Affinis total flavones according to claim 1; It is characterized in that: said lyophilization be with concentrated solution freezer dryer vacuum less than 20Pa, condenser temperature-45 ℃～-60 ℃ conditions under drying totally, Herba Gnaphalii Affinis total flavones product.
6. the method for distilling of a kind of Herba Gnaphalii Affinis total flavones according to claim 1 is characterized in that: the total flavones product purity of said Herba Gnaphalii Affinis total flavones product is greater than 3.5%.
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| CN201110177622.8A CN102836194B (en) | 2011-06-21 | 2011-06-21 | Extraction method of affine cudweed total flavonoids |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142742A (en) * | 2013-03-21 | 2013-06-12 | 河北康利动物药业有限公司 | Method for preparing oral solution of poplar flower |
| CN103919977A (en) * | 2014-04-29 | 2014-07-16 | 韦江南 | Chinese herbal preparation for treating hyperlipemia |
| CN106858264A (en) * | 2016-12-26 | 2017-06-20 | 福建省龙海市安利达工贸有限公司 | A kind of preparation method of affine cudweed antioxidant |
| CN107094959A (en) * | 2017-06-30 | 2017-08-29 | 邝义槐 | A kind of herbal tea with hypoglycemic effect and preparation method thereof |
| CN108464482A (en) * | 2018-03-05 | 2018-08-31 | 周祥宝 | A kind of preparation method of affine cudweed toppings |
| CN111870649A (en) * | 2020-09-04 | 2020-11-03 | 杨鹏 | Traditional Chinese medicine composition for treating type II diabetes and preparation method thereof |
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| CN1195545A (en) * | 1998-04-14 | 1998-10-14 | 梅景森 | Plaster for curing cough and asthma and its producing method |
| CN101019906A (en) * | 2007-03-19 | 2007-08-22 | 沈信堂 | Chinese medicine for relieving cough, eliminating phlegm and relieving asthma |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142742A (en) * | 2013-03-21 | 2013-06-12 | 河北康利动物药业有限公司 | Method for preparing oral solution of poplar flower |
| CN103919977A (en) * | 2014-04-29 | 2014-07-16 | 韦江南 | Chinese herbal preparation for treating hyperlipemia |
| CN106858264A (en) * | 2016-12-26 | 2017-06-20 | 福建省龙海市安利达工贸有限公司 | A kind of preparation method of affine cudweed antioxidant |
| CN107094959A (en) * | 2017-06-30 | 2017-08-29 | 邝义槐 | A kind of herbal tea with hypoglycemic effect and preparation method thereof |
| CN108464482A (en) * | 2018-03-05 | 2018-08-31 | 周祥宝 | A kind of preparation method of affine cudweed toppings |
| CN111870649A (en) * | 2020-09-04 | 2020-11-03 | 杨鹏 | Traditional Chinese medicine composition for treating type II diabetes and preparation method thereof |
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| CN102836194B (en) | 2014-04-09 |
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