CN102828346A - Method for preparing galactose homopolymer/polyacrylonitrile composite nanofiber membrane - Google Patents
Method for preparing galactose homopolymer/polyacrylonitrile composite nanofiber membrane Download PDFInfo
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Abstract
The invention discloses a method for preparing a galactose homopolymer/polyacrylonitrile composite nanofiber membrane, which comprises the following steps that: (1) diacid divinyl ester and galactose are dissolved in anhydrous pyridine, and alkaline protease is added to synthesize galactose vinyl ester; (2) ammonium persulfate is added into the galactose to serve as an initiator, H2O or oil is added to serve as solvent, and after the polymerization reaction, Poly-OVSEGA is obtained; (3) polyacrylonitrile is dissolved in DMF (dimethyl formamide), the Poly-OVSEGA is then added to be mixed until complete dissolving, and the mixture is stood to obtain PAN (polyacrylonitrile)/Poly-OVSEGA spinning solution; and (4) the PAN/Poly-OVSEGA spinning solution is subjected to electrostatic spinning, and is finally dried, so the galactose homopolymer/polyacrylonitrile composite nanofiber membrane is obtained. The method for producing the galactose homopolymer/polyacrylonitrile composite nanofiber membrane is simple to operate, and the product is easy to processs, is economic and environment-friendly; and the membrane contains abundant functional groups which can reflect the hydrophic activity, can be used for protein separation and purification, and is low in cost.
Description
Technical field
The invention belongs to the preparation field of nano fibrous membrane, the preparation method of particularly a kind of galactolipin homopolymers/polyacrylonitrile blending nano fibrous membrane.
Background technology
Sugar and protein, lipid is the same with nucleic acid, is the important component that constitutes organism.At cell construction, in the biosynthesis of cell and the regulation and control of cell activities, sugar is all being played the part of important role.Sugar not only can be participated in life process directly with the form of polysaccharide or free oligosaccharides, and can be used as saccharide complex, participates in targeting proteins, important physiological processes such as cell recognition and antibody-AI like glycoprotein, proteoglycan and glycolipid etc.What this noun of glycobiology was also write professor Dwek of Oxford University in 1988 is to put forward in the summary of topic with " glycobiology ", indicates the birth of this new subdiscipline of glycobiology.
" the sugar quilt " of biofilm surface given biomembrane good hydrophilicity and biologic specificity, not only has the performance of resisting the non-specific adhesion of foreign substance, also has the function of selectivity identification and absorption target substance." sugar by " fine and close glycosylation layer structure significantly strengthened the affinity between it and target molecule; Satisfied the demand of each item physiological activity; Make up the glycosylation layer that is similar to " sugar quilt " on the polymeric membrane for separation surface; Human simulation be can realize, high molecular range of application and field expanded biofilm surface.In this year, people are fixed to the different carriers surface with glycosyl, simulate the various biological functions of sugar, thereby the compatibility of further investigation sugar and protein interacts.Disclose in the U.S. Pat 20010017270 glycosyl has been fixed to gold surface, available about protein, the detection technique of virus or cell.Patent CN1935342 and CN101070401 disclose the method that glycosyl is fixed to polypropylene separation membrane surface and polypropylene microsphere surface respectively, help the separation of protein, concentrate or the target removing.
Electrostatic spinning is that a kind of charged Polymer Solution or melt of utilizing flows in electrostatic field and the technology of distortion preparation nanometer to the submicron-scale polymer fiber.Polymer fiber with respect to the conventional method preparation; The fiber that electro-spinning is equipped with has great specific area; Advantages such as high porosity; Nano fibrous membrane can reduce the resistance to mass tranfer in the separation process greatly because be a kind of good adsorption and separation material, particularly for the identification of protein with separate.Publication number be open in the patent documentation of CN1843592A be raw material with the glycosylation acrylonitrile copolymer, prepared through method of electrostatic spinning and contained sugared nano fibrous membrane, the preparation method is simple, and protein is had certain specific recognition effect.Characteristics such as electrostatic spinning also has simply, convenience, cheapness, thus people evoked to its great interest of furtheing investigate.
Polyacrylonitrile (PAN) is one type of commodity polymeric material, good, the anti-common solvent of its fibre forming property, not facile hydrolysis, anti-oxidant, chemically stable, and have excellent anti-bacterium aggressivity.Utilize electrostatic spinning technique, can prepare the PAN nanofiber simply and easily; The formed nano fibrous membrane of the unordered accumulation of PAN nanofiber then has high specific area and porosity, has mechanical strength preferably simultaneously, and its fibre morphology can also be regulated and control through preparation condition.These characteristics make this material demonstrate potential application foreground in fields such as bioengineering, composite, isolated by filtration, sensor and photoelectric devices.
Because polyacrylonitrile surface relative inertness and hydrophily are relatively poor; When separating as the aqueous solution, meeting and biomolecule generation non-specific interaction cause protein, blood platelet etc. in a large amount of absorption in film surface; Cause film to pollute, have a strong impact on the military service stability of film.Therefore, be necessary the polyacrylonitrile microporous barrier is carried out surface modification, improve the hydrophily of film on the one hand, improve the contamination resistance of film, increase the service life; Give film on the other hand with new function, widen its range of application.
Summary of the invention
Technical problem to be solved by this invention provides a kind of galactolipin homopolymers/polyacrylonitrile composite nano-fiber membrane preparation method; This method is simple to operate, product is prone to handle and economic environmental protection; But this tunica fibrosa contains abundant reactive hydrophilic activated functional groups, can be used for the separation and purification of protein.
A kind of galactolipin homopolymers of the present invention/polyacrylonitrile composite nano-fiber membrane preparation method comprises:
(1) diacid divinyl ester and galactolipin are dissolved in 50～100mL anhydrous pyridine, add 0.5～1.5g alkali protease, in 40～60 ℃ of constant-temperature shaking culture casees, react 3～5 days (rotating speed is 210rpm), synthetic galactolipin ethene fat;
(2) in above-mentioned galactolipin ethene fat, add ammonium persulfate as initator, add H
2O or oil are made solvent, and stirring reaction 3～6h under 55～70 ℃ of nitrogen protections obtains galactolipin ethene fat homopolymers Poly-OVSEGA after the polymerisation end then;
(3) polyacrylonitrile (PAN) is dissolved in DMF, obtaining concentration is the PAN solution of 0.03～0.2g/mL, adds above-mentioned Poly-OVSEGA then, is stirred to dissolving fully, gets the PAN/Poly-OVSEGA spinning solution after leaving standstill;
(4) above-mentioned PAN/Poly-OVSEGA spinning solution is carried out electrostatic spinning, final drying promptly gets galactolipin homopolymers/polyacrylonitrile composite nano-fiber membrane.
The mol ratio of diacid divinyl ester described in the step (1) and galactolipin is 1:1～4:1.
The consumption of the ammonium persulfate described in the step (2) accounts for 0.2～2% of galactolipin ethene lipid amount mark.
Adding H described in the step (2)
2After O or oil were made solvent, monomer (galactolipin ethene fat) concentration was 2～3mol/L.
To account for the mass fraction of polyacrylonitrile (PAN) and Poly-OVSEGA quality sum be 10～90% to Poly-OVSEGA in the PAN/Poly-OVSEGA spinning solution described in the step (3).
To account for the mass fraction of polyacrylonitrile (PAN) and Poly-OVSEGA quality sum be 40%, 50% or 60% to Poly-OVSEGA in the PAN/Poly-OVSEGA spinning solution described in the step (3).
The technological parameter of the electrostatic spinning described in the step (4) is: the syringe specification is 5mL; The syringe needle internal diameter is 0.4～0.7mm, ejection flow velocity 0.8～2mL/h, electrostatic pressure 10～18kV; Receiving screen adopts the reception of aluminium foil ground connection; Accepting distance is 10～20cm, adopts orthogonal method (change a certain parameter, fix other parameter) adjusting spinning parameter to carry out electricity and spin.
The separation and purification of the resulting galactolipin homopolymers of step (4)/polyacrylonitrile composite nano-fiber membrane The proteins.
Contain sugared polyacrylonitrile nano tunica fibrosa with what the present invention obtained, utilize the Bradford method to detect the ability of its anti-non-specific adsorption.
BSA is dissolved in the PBS cushioning liquid of pH=7.0～7.4, is configured to the solution of variable concentrations.According to above-mentioned Bradford method,, prepare the BSA absorbance calibration curve of this concentration range with the absorbance of ultraviolet specrophotometer and 595nm place detection variable concentrations BSA standard liquid.Get the film to be measured of certain area; After ethanol and the processing of PBS buffer solution, adding 1～10mL concentration is the BSA solution of 0～1.0mg/mL, constant temperature 12～48h in 20-50 ℃ of constant temperature water bath oscillator; After treating adsorption equilibrium; Directly measure the absorption of solution at the 595nm place with the Bradford method, the reference standard curve gets final product the amount that the BSA on the unit of account membrane area adsorbs.
Can draw the absorption that sugared polyacrylonitrile nano tunica fibrosa can resist nonspecific proteins matter that contains that the present invention obtains.
The present invention carries out blending through preparation galactosaccharic acid divinyl ester homopolymer (Poly-OVSEGA) and polyacrylonitrile (PAN), and carbohydrate is joined on the nano fibrous membrane.The specific area that combining nano fiber of the present invention is high and the high-hydrophilic of glycolipid help bringing into play the collection cluster effect of glycosyl, thereby have improved the performance of anti-specific proteins absorption.Advantages such as it is simple that glycosyl modified polyacrylonitrile fibre has preparation, can be repeatedly used, with low cost.
The surface of sugar introducing material, with the biocompatibility that effectively improves material surface.Therefore, sugar-containing polymer often is used to improve the non-specific adsorption of the material such as hydrophily and CKIs matter of material at material surface.
The present invention obtains diameter and aperture at nano level membrane material PAN/ galactolipin ethene fat superfine nano tunica fibrosa with polyacrylonitrile and galactolipin ethene lipid blend.This membrane material has overcome the intrinsic defective of polyacrylonitrile, but contains abundant reactive hydrophilic activated functional groups, has improved the non-specific adsorption of material surface and has improved the biocompatibility of material surface, has to use it and do the potentiality that follow-up related experiment is analyzed.
The present invention is main spinning material with PAN; And mix the good synthetic galactolipin ethene fat homopolymers of surfaces for biocompatibility; Through adjustment solvent and relevant spinning condition parameter, successful realization blending, for the research and development of novel nano bioactive materials are offered reference.
Beneficial effect:
(1) the present invention adopts the aqueous phase precipitation polymerization to prepare galactolipin ethene fat homopolymers, and the preparation method is simple to operate, product is prone to handle and economic environmental protection;
(2) membrane material of the present invention has overcome the intrinsic defective of polyacrylonitrile; But contain abundant reactive hydrophilic activated functional groups; Improved the non-specific adsorption of material surface and improved the biocompatibility of material surface, had and use it and do the potentiality that follow-up related experiment is analyzed;
(3) contain the separation and purification that sugared polyacrylonitrile nano tunica fibrosa can be used for protein, can be repeatedly used, with low cost.
Description of drawings
Fig. 1 is the stereoscan photograph that contains sugared polyacrylonitrile nano tunica fibrosa of embodiment 1～3, Comparative Examples 1 gained; Wherein the stereoscan photograph that contains sugared polyacrylonitrile nano tunica fibrosa of embodiment 1～3, Comparative Examples 1 is respectively A; B, C, (spinning voltage is 16kV to D; Receiving range is 15cm, and the injection flow velocity is 1.5mL/h);
Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D are respectively the distribution of fiber diameters that contains sugared polyacrylonitrile nano tunica fibrosa for embodiment 1～3, Comparative Examples 1 gained, wherein Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D respectively with Fig. 1 in A, B, C, D corresponding;
Fig. 3 is the calibration curve of BSA absorption.
Fig. 4 be embodiment 1～3, Comparative Examples 1 gained contain the adsorption curve of sugared polyacrylonitrile nano tunica fibrosa to BSA.
Fig. 5 is a galactolipin decanedioic acid divinyl fat, Poly-OVSEGA, and the FT-IR IR Characterization of PAN/Poly-OVSEGA nano fibrous membrane, wherein 5A is a galactolipin decanedioic acid divinyl fat, and 5B is the galactolipid homopolymers, and 5C is galactolipin homopolymers/PAN nano fibrous membrane.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) diacid divinyl ester and the galactolipin molar ratio by 3:1 is dissolved among the anhydrous pyridine 100mL; Add the 0.5g alkali protease; Reaction is 4 days in 40 ℃ of constant-temperature shaking culture casees, and rotating speed is 210rpm, utilizes the enzymatic synthetic technology to synthesize galactolipin ethene fat.
(2) above-mentioned glycolipid is put in the micro-polymerization pipe, as initator, adds H with ammonium persulfate (APS) (accounting for galactolipin ethene lipid amount mark is 1.0)
2O makes solvent, and (monomer concentration is 2.0mol/L H
2O), sealing vacuumizes the logical nitrogen in back, repeated multiple times with oil pump.System is placed stirring reaction 5h under 55～60 ℃ of nitrogen protections.
(3) PAN of certain mass is dissolved in DMF and obtains the PAN solution that concentration is 0.1g/mL; Add the homopolymerization of gala fat then, make its shared mass fraction be respectively 40%, stirred for several hour is to dissolving fully, leaves standstill bubble to be stirred fully form the homogeneous spinning solution.
(4) extract PAN/ galactolipin ethene fat homopolymers with syringe, be fixed on the electrostatic spinning apparatus, control ejection flow velocity 1.5mL/h; Electrostatic pressure 16kV; Receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10～20cm, adopts orthogonal method (to change a certain parameter; Fixing other parameter) regulates different spinning parameters and carry out electricity and spin, obtain PAN/ galactolipin ethene fat homopolymers superfine nano tunica fibrosa; Put into 60～80 ℃ of freeze-day with constant temperature 36h of vacuum drying chamber, process the regeneration nano composite membrane, its electromicroscopic photograph is shown in Figure 1A.
(1) diacid divinyl ester and the galactolipin molar ratio by 3:1 is dissolved among the anhydrous pyridine 100mL; Add the 1.0g alkali protease; Reaction is 4 days in 45 ℃ of constant-temperature shaking culture casees, and rotating speed is 210rpm, utilizes the enzymatic synthetic technology to synthesize galactolipin ethene fat.
(2) above-mentioned glycolipid is put in the micro-polymerization pipe, as initator, adds H with ammonium persulfate (APS) (accounting for galactolipin ethene lipid amount mark is 1.0)
2O makes solvent, and (monomer concentration is 2.0mol/L H
2O), sealing vacuumizes the logical nitrogen in back, repeated multiple times with oil pump.System is placed stirring reaction 5h under 55～60 ℃ of nitrogen protections.
(3) PAN of certain mass is dissolved in DMF and obtains the PAN solution that concentration is 0.1g/mL; Add the galactolipin homopolymerization then, make its shared mass fraction be respectively 50%, stirred for several hour is to dissolving fully, leaves standstill bubble to be stirred fully form the homogeneous spinning solution.
(4) extract PAN/ galactolipin ethene fat homopolymers with syringe, be fixed on the electrostatic spinning apparatus, control ejection flow velocity 1.5mL/h; Electrostatic pressure 16kV; Receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10～20cm, adopts orthogonal method (to change a certain parameter; Fixing other parameter) regulates different spinning parameters and carry out electricity and spin, obtain PAN/ galactolipin ethene fat homopolymers superfine nano tunica fibrosa; Put into 60～80 ℃ of freeze-day with constant temperature 24h of vacuum drying chamber, process the regeneration nano composite membrane, its electromicroscopic photograph is shown in Figure 1B.
Embodiment 3
(1) diacid divinyl ester and the galactolipin molar ratio by 3:1 is dissolved among the anhydrous pyridine 100mL; Add the 1.5g alkali protease; Reaction is 4 days in 60 ℃ of constant-temperature shaking culture casees, and rotating speed is 210rpm, utilizes the enzymatic synthetic technology to synthesize galactolipin ethene fat.
(2) above-mentioned glycolipid is put in the micro-polymerization pipe, as initator, adds H with ammonium persulfate (APS) (accounting for galactolipin ethene lipid amount mark is 1.0)
2O makes solvent, and (monomer concentration is 2.0mol/L H
2O), sealing vacuumizes the logical nitrogen in back, repeated multiple times with oil pump.System is placed stirring reaction 4h under 55～60 ℃ of nitrogen protections.
(3) PAN of certain mass is dissolved in DMF and obtains the PAN solution that concentration is 0.1g/mL; Add the homopolymerization of galactolipin ethene fat then, make its shared mass fraction be respectively 60%, stirred for several hour is to dissolving fully, leaves standstill bubble to be stirred fully form the homogeneous spinning solution.
(4) extract PAN/ galactolipin ethene fat homopolymers with syringe, be fixed on the electrostatic spinning apparatus, control ejection flow velocity 1.5mL/h; Electrostatic pressure 16kV; Receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10～20cm, adopts orthogonal method (to change a certain parameter; Fixing other parameter) regulates different spinning parameters and carry out electricity and spin, obtain PAN/ galactolipin ethene fat homopolymers superfine nano tunica fibrosa; Put into 60～80 ℃ of freeze-day with constant temperature 30h of vacuum drying chamber, process the regeneration nano composite membrane, its electromicroscopic photograph is shown in Fig. 1 C.
Embodiment 4
Galactolipin homopolymerization/polyacrylonitrile blending nano fibrous membrane is to the absorption of BSA
In 0～100ug/mL scope, draw BSA solution calibration curve.Prepare the BSA standard liquid of series concentration respectively with the PBS of pH=7.4, utilize the Bradford method, detect the absorbance of variable concentrations BSA standard liquid with ultraviolet specrophotometer 595nm place.Be ordinate then with the absorbance, BSA concentration is abscissa, and the BSA absorbance calibration curve of this concentration range of system is like Fig. 3.
Galactolipin homopolymerization/polyacrylonitrile blending the nano fibrous membrane of embodiment 1～3 preparation is successively used PBS cushioning liquid preliminary treatment (20～30min); To remove the pollutant on striping surface; Get 0.5mg film to be measured; Put into test tube after shredding, add the BSA solution of 1～10mL same concentrations respectively, at 3 ℃ of round waters constant temperature oscillator constant temperature 24h.After treating adsorption equilibrium, take out film, solution is in the absorption at 595nm place in the mensuration test tube.The reference standard curve changes the pairing concentration of light absorption value under several.According to the minimizing of concentration, come the amount of BSA absorption on the film of unit of account quality; Adsorption curve such as Fig. 3.
Comparative Examples 1
(1) PAN with certain mass is dissolved among the DMF.Stirred for several hour is to dissolving fully, leaves standstill bubble to be stirred fully form the homogeneous spinning solution, and obtaining concentration is the PAN solution of 10%g/mL.
(2) extract the PAN spinning solution on electrostatic spinning apparatus with syringe; Control ejection flow velocity 1.0～1.5mL/h, electrostatic pressure 10～18kV, receiving screen adopts the reception of aluminium foil ground connection; The distance of syringe needle and receiving screen is 10～20cm; Adopt orthogonal method (change a certain parameter, fix other parameter) the different spinning parameters of adjusting to carry out electricity and spin, obtain PAN superfine nano tunica fibrosa; Put into 60～80 ℃ of freeze-day with constant temperature 36h of vacuum drying chamber, process the regeneration nano composite membrane, its electromicroscopic photograph is shown in Fig. 1 D.
Claims (8)
1. galactolipin homopolymers/polyacrylonitrile composite nano-fiber membrane preparation method comprises:
(1) diacid divinyl ester and galactolipin are dissolved in anhydrous pyridine, add alkali protease, in 40～60 ℃ of reactions 3～5 days, synthetic galactolipin ethene fat;
(2) in above-mentioned galactolipin ethene fat, add ammonium persulfate as initator, add H
2O or oil are made solvent, and stirring reaction 3～6h under 55～70 ℃ of nitrogen protections obtains galactolipin ethene fat homopolymers Poly-OVSEGA after the polymerisation end then;
(3) polyacrylonitrile (PAN) is dissolved in DMF, obtaining concentration is the PAN solution of 0.03～0.2g/mL, adds above-mentioned Poly-OVSEGA then, is stirred to dissolving fully, gets the PAN/Poly-OVSEGA spinning solution after leaving standstill;
(4) above-mentioned PAN/Poly-OVSEGA spinning solution is carried out electrostatic spinning, final drying promptly gets galactolipin homopolymers/polyacrylonitrile composite nano-fiber membrane.
2. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method, it is characterized in that: the mol ratio of diacid divinyl ester described in the step (1) and galactolipin is 1:1～4:1.
3. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method is characterized in that: the consumption of the ammonium persulfate described in the step (2) accounts for 0.2～2% of galactolipin ethene lipid amount mark.
4. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method is characterized in that: the adding H described in the step (2)
2After O or oil were made solvent, galactolipin ethene lipid concentration was 2～3mol/L.
5. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method is characterized in that: to account for the mass fraction of polyacrylonitrile (PAN) and Poly-OVSEGA quality sum be 10～90% to Poly-OVSEGA in the PAN/Poly-OVSEGA spinning solution described in the step (3).
6. according to claim 1 or 5 described a kind of galactolipin homopolymers/polyacrylonitrile composite nano-fiber membrane preparation methods, it is characterized in that: to account for the mass fraction of polyacrylonitrile (PAN) and Poly-OVSEGA quality sum be 40%, 50% or 60% to Poly-OVSEGA in the PAN/Poly-OVSEGA spinning solution described in the step (3).
7. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method; It is characterized in that: the technological parameter of the electrostatic spinning described in the step (4) is: the syringe specification is 5mL; The syringe needle internal diameter is 0.4 ~ 0.7mm, ejection flow velocity 0.8～2mL/h, electrostatic pressure 10～18kV; Receiving screen adopts the reception of aluminium foil ground connection, and accepting distance is 10～20cm.
8. a kind of galactolipin homopolymers according to claim 1/polyacrylonitrile composite nano-fiber membrane preparation method is characterized in that: the separation and purification of the resulting galactolipin homopolymers of step (4)/polyacrylonitrile composite nano-fiber membrane The proteins.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103074733A (en) * | 2012-12-31 | 2013-05-01 | 东华大学 | Method for preparing thermosensitive galactose vinyl ester nano drug-carrying fiber membrane through electrostatic spinning |
| CN103194857A (en) * | 2013-04-12 | 2013-07-10 | 东华大学 | Preparation method of electrostatic spinning glucose ether/acrylonitrile copolymer nanofiber membrane |
| CN103614859A (en) * | 2013-11-26 | 2014-03-05 | 东华大学 | Method for preparing glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane |
| CN103614860A (en) * | 2013-11-26 | 2014-03-05 | 东华大学 | Method for preparing glucose azelaic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane |
| CN105155139A (en) * | 2015-06-11 | 2015-12-16 | 浪莎针织有限公司 | Preparation method for electrostatic spinning glucose ester/acrylonitrile copolymer nanofiber membrane and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843592A (en) * | 2006-03-22 | 2006-10-11 | 浙江大学 | Method for preparing nanometer fibrous film of glycosylated acrylon and application thereof |
| CN101285221A (en) * | 2008-05-14 | 2008-10-15 | 浙江大学 | Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications |
| CN102115921A (en) * | 2010-12-22 | 2011-07-06 | 浙江大学 | Glycosylated nanofiber membrane and preparation method and application thereof |
| CN102418237A (en) * | 2011-08-12 | 2012-04-18 | 东华大学 | Preparation method for electrostatic blended sugar-containing polyacrylonitrile (PAN) nano-fiber membrane |
-
2012
- 2012-07-26 CN CN2012102607378A patent/CN102828346A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843592A (en) * | 2006-03-22 | 2006-10-11 | 浙江大学 | Method for preparing nanometer fibrous film of glycosylated acrylon and application thereof |
| CN101285221A (en) * | 2008-05-14 | 2008-10-15 | 浙江大学 | Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications |
| CN102115921A (en) * | 2010-12-22 | 2011-07-06 | 浙江大学 | Glycosylated nanofiber membrane and preparation method and application thereof |
| CN102418237A (en) * | 2011-08-12 | 2012-04-18 | 东华大学 | Preparation method for electrostatic blended sugar-containing polyacrylonitrile (PAN) nano-fiber membrane |
Non-Patent Citations (1)
| Title |
|---|
| YAN LI ET ALS: "Chemo-enzymatic synthesis of nanofiber membrane with glucose by electrospinning", 《THE 5TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103074733A (en) * | 2012-12-31 | 2013-05-01 | 东华大学 | Method for preparing thermosensitive galactose vinyl ester nano drug-carrying fiber membrane through electrostatic spinning |
| CN103074733B (en) * | 2012-12-31 | 2016-04-06 | 东华大学 | A kind of electro-spinning is for the method for Thermo-sensitive galactolipin vinyl acetate nano drug-carrying tunica fibrosa |
| CN103194857A (en) * | 2013-04-12 | 2013-07-10 | 东华大学 | Preparation method of electrostatic spinning glucose ether/acrylonitrile copolymer nanofiber membrane |
| CN103614859A (en) * | 2013-11-26 | 2014-03-05 | 东华大学 | Method for preparing glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane |
| CN103614860A (en) * | 2013-11-26 | 2014-03-05 | 东华大学 | Method for preparing glucose azelaic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane |
| CN105155139A (en) * | 2015-06-11 | 2015-12-16 | 浪莎针织有限公司 | Preparation method for electrostatic spinning glucose ester/acrylonitrile copolymer nanofiber membrane and application thereof |
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