CN102809626A - Quality control method of Qianshan Huoxue plaster - Google Patents
Quality control method of Qianshan Huoxue plaster Download PDFInfo
- Publication number
- CN102809626A CN102809626A CN2012103017128A CN201210301712A CN102809626A CN 102809626 A CN102809626 A CN 102809626A CN 2012103017128 A CN2012103017128 A CN 2012103017128A CN 201210301712 A CN201210301712 A CN 201210301712A CN 102809626 A CN102809626 A CN 102809626A
- Authority
- CN
- China
- Prior art keywords
- solution
- ginsenoside
- blood circulation
- cream
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a quality control method of a Qianshan Huoxue plaster. The Qianshan Huoxue plaster is recorded in national Chinese patent drug local standard WS-10415(ZD-0415)-2002. The quality control method of a Chinese patent drug comprises the following steps of: identifying dragon's blood and pseudo-ginseng crude drugs in a prescription through thin-layer chromatography; and determining ginsenoside Rg1 and Rb1 and notoginsenoside R1, which are contained by the pseudo-ginseng in the prescription through high performance liquid chromatography. The content is determined by using a high performance liquid chromatography-evaporative light scattering detection method, the defect that the conventional quality standard content determination is influenced by a solvent due to adoption of a 203nm ultrasonic detector is overcome, the quality control method is good in effect, and the quality of a product can be well controlled.
Description
Technical field
The invention belongs to medical technical field; Exactly; Relate to a kind of numerous mountains the invigorate blood circulation control method, particularly numerous mountains of cream Chinese medicine drug quality invigorate blood circulation ginsenoside Rg1 in the paste formulation prescription pseudo-ginseng, Rb1 and three kinds of composition high performance liquid chromatography of notoginsenoside R content assaying method.
Background technology
The numerous mountains cream of invigorating blood circulation is made up of ground bettle, rheum officinale, pseudo-ginseng, corydalis tuber, dragon's blood, teasel root, golden cypress, frankincense, myrrh, catechu, the root of Chinese wild ginger, rhizoma homalonemae, edible tulip, rhizoma alismatis, antelope's horn, the banksia rose, bletilla, the root of Dahurain angelica, cassia twig, notopterygium root 20 flavor medicines; Record in provincial standard WS-10415 (ZD-0415)-2002; Have promoting blood circulation and removing blood stasis, stimulate the circulation of the blood and cause the muscles and joints to relax, the effect of swelling and pain relieving; Be used for skin, arthroncus, pain, movable unfavorable and traumatic injury, diseases such as waist, knee Osteoarthritis.
The numerous mountains cream proper mass standard of invigorating blood circulation has been carried out the discriminating of thin-layer chromatography to dragon's blood, pseudo-ginseng, and the people's soap saponin(e Rg1 in the pseudo-ginseng has been carried out assay, and it is few to detect index; Be difficult to control product quality comprehensively; Particularly contained ginsenoside Rg1 and ginsenoside Rb1, notoginsenoside is the tetracyclic triterpene saponins in the pseudo-ginseng, in the ultraviolet region is terminal the absorption, and be approaching with the uv absorption wavelength of some organic solvents commonly used; Influence is measured; Use the low wavelength of ultraviolet to detect, can bring certain difficulty, influence the accuracy and the reappearance of assay to mensuration.
It is a kind of novel universal detecting device that detecting device (ELSD) is penetrated in the evaporation color break-up, and its signal response is directly proportional with the quality of measured object, and does not rely on the optical characteristics and the functional group of measured object, can be used for the detection that volatility is lower than any component of moving phase in theory.It has response preferably to no uv absorption or the material that absorbs for ultraviolet is terminal such as carbohydrate, saponins, steroid class etc.The baseline wander that ELSD has eliminated the interference of solvent and caused because of temperature variation promptly uses gradient elution also can not produce baseline wander.The response of ELSD does not rely on the optical property of sample; Can be to be detected as long as the volatility of sample is lower than moving phase; Be particularly useful for moving phase the situation that uv absorption is disturbed or the drift of gradient elution base line influences is arranged; Overcome the deficiency of traditional detection method, increasing being applied in the high performance liquid chromatography.The maximum superiority of EISD (ELSD) is to detect and does not contain chromophoric compound; The universal test method of ELSD has been eliminated the difficult point that is common in traditional HPLC detection method; Be different from ultraviolet and fluorescence detector; The response of ELSD does not rely on the optical characteristics with sample, and any volatility is lower than the sample of moving phase all can be to be detected, also can use at low temperatures.EISD baseline noise is little, not influenced by gradient, and peak shape is sharp-pointed, and impurity peaks seldom.And the UV-detector baseline is influenced by gradient, and baseline wander is bigger in analytic process.The lower ultraviolet detection wavelength (203nm) of the normal use of the analysis of saponin component in the pseudo-ginseng, this wave band is terminal the absorption, noise signal is very big, is difficult to obtain satisfied result.The ELSD detecting device only produces signal to non-volatile analyte as a kind of common detector, and its signal response value only depends on the size and the quantity of analyte particle, does not have the terminal problem that absorbs of ultraviolet.And the variation to moving phase is insensitive, even also very steady at the gradient elution base line.Through regulating the drift tube temperature and the flow rate of carrier gas of ELSD detecting device, can make the baseline noise minimum, response is the highest relatively, thereby obtains good chromatogram.
Summary of the invention
The object of the present invention is to provide the invigorate blood circulation method of quality control of cream of a kind of new numerous mountains.
The present invention realizes through following technical scheme
The numerous mountains of the present invention cream method of quality control of invigorating blood circulation comprises following discriminating and/or content assaying method:
Discriminating comprises following method:
(1) get these article 5g, add ethanol 10ml, sonicated 15 minutes is put coldly, filters, and gets filtrating, as need testing solution.Other gets dragon's blood control medicinal material 0.1g, adds ethanol 5ml and makes dissolving, as control medicinal material solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developping agent with chloroform-methanol (19: 1), launch, take out, dry.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the principal spot of same color.
(2) get these article 10g, add water 70ml, heated and boiled 30 minutes is put coldly, filters; The filtrating 30ml jolting that adds diethyl ether is extracted, and discards ether solution, and water layer extracts 2 times with water saturated normal butyl alcohol jolting, at every turn 40ml; Merge normal butyl alcohol liquid,, discard ammoniacal liquor, again with the saturated water 30ml washing of normal butyl alcohol with ammonia solution 30ml washing; Discard water liquid, normal butyl alcohol liquid evaporate to dryness, residue add methyl alcohol 1ml makes dissolving, as need testing solution; Other gets pseudo-ginseng control medicinal material 1g, adds methyl alcohol 20ml, and refluxing extraction 1 hour is put coldly, filter, and the filtrating evaporate to dryness, residue adds water 20ml makes dissolving, extracts 2 times with water saturated normal butyl alcohol jolting, each 25ml, merging normal butyl alcohol liquid shines medicinal material solution in pairs with legal system; Get ginsenoside Rb1, Rg1 and notoginsenoside R reference substance again, add methyl alcohol and process the mixed solution that every 1ml contains 1mg, as reference substance solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate; With 10 ℃ of lower floor's solution with the held layering of chloroform-methanol-water (13: 7: 2) is developping agent; Launch, take out, dry; Spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the spot colour developing at 105 ℃.Put respectively under daylight and the ultraviolet lamp (365nm) and inspect, in the test sample chromatogram.With control medicinal material and the corresponding position of reference substance chromatogram on, show the spot or the fluorescence spot of same color respectively.
Assay comprises following method:
Chromatographic condition and system suitability test are filling agent with the octadecylsilane chemically bonded silica; With the acetonitrile-water is moving phase, and according to the form below carries out gradient elution:
The evaporation color break-up is penetrated the detecting device condition and is: drift tube temperature is 120 ℃, and flow rate of carrier gas is 2.0L/min; Number of theoretical plate calculates by the ginsenoside Rg1 peak should be not less than 2000.
The preparation of reference substance solution: precision takes by weighing that to be dried to ginsenoside Rg1, ginsenoside Rb1, the notoginsenoside R reference substance of constant weight through phosphorus pentoxide an amount of; Add methyl alcohol and process the mixed solution that every 1ml contains ginsenoside Rg1 0.5mg/ml, ginsenoside Rb10.5mg/ml, notoginsenoside R 0.15mg/ml, as reference substance solution.
The preparation of need testing solution: get the numerous mountains cream of invigorating blood circulation, remove the lid lining, get that 2.5g is accurate to be claimed surely, put in the apparatus,Soxhlet's refluxing extraction that adds diethyl ether 3 hours; Discard ether solution, the dregs of a decoction volatilize, and add methanol eddy and extract 4 hours, and methanol extract liquid evaporate to dryness, residue add the water low-grade fever makes dissolving; Put cold, last macroporous adsorptive resins (internal diameter 1.5cm, long 10cm), 20% the ethanolic solution washing with 2-4 times of column volume discards 20% ethanol eluate; Use 70% ethanol 5-8 times of column volume wash-out again, collect eluent, water bath method, residue is used dissolve with methanol; Be transferred in the 5ml volumetric flask, be diluted to scale, shake up, as need testing solution with methyl alcohol.
Accurate respectively reference substance solution 5 μ l, 10 μ l and the need testing solution 10 μ l of drawing of determination method inject liquid chromatograph, measure, and promptly get.
Further explain in the face of the numerous mountains of the present invention cream method of quality control of invigorating blood circulation down.
Invigorate blood circulation cream content assaying method test of numerous mountains
1, instrument and reagent
High performance liquid chromatograph: day island proper Tianjin company (LC-2010A); Detecting device: EISD (SEDEX75); The second eyeball is a chromatographically pure, Dikma company, and water is ultrapure water.
The reference substance notoginsenoside R (batch: 110745-201012), the ginsenoside Rg1 (lot number: 0703-201018), the ginsenoside Rb1 (lot number: 110704-201018), available from Nat'l Pharmaceutical & Biological Products Control Institute.
2, method and result
Chromatographic condition: use octadecylsilane chemically bonded silica to be filling agent; Dikma 5 μ m, 150mm*4.6mm; Moving phase is carried out gradient elution for the acetonitrile-water according to the form below:
It is 120 ℃ that the detecting device drift tube temperature is penetrated in the evaporation color break-up, and flow rate of carrier gas is 2.0L/min.
The preparation of reference substance solution: precision takes by weighing that to be dried to ginsenoside Rg1, ginsenoside Rb1, the notoginsenoside R reference substance of constant weight through phosphorus pentoxide an amount of; Add methyl alcohol and process the mixed solution that every 1ml contains ginsenoside Rg1 0.5mg/ml, ginsenoside Rb10.5mg/ml, notoginsenoside R 0.15mg/ml, as reference substance solution.
The preparation of need testing solution: get the numerous mountains cream of invigorating blood circulation, remove the lid lining, the cream 2.5g that gets it filled, accurate claim fixed; Put in the apparatus,Soxhlet's, extremely colourless with extracted by ether earlier, after the medicine residue dries, utilize apparatus,Soxhlet's extremely colourless again with methanol extraction; The methanol extract liquid water bath method, residue water low-grade fever makes dissolving, crosses macroporous adsorptive resins (internal diameter 1.5cm, long 10cm); 20% ethanolic solution washing with 2-4 times of column volume discards 20% ethanol eluate, uses 70% ethanol 5-8 times of column volume wash-out again, collects eluent; Water bath method, residue is used dissolve with methanol, with methyl alcohol dilution and constant volume in the 5ml volumetric flask, as need testing solution.
Measure: accurate respectively reference substance solution 5,10ul and the need testing solution 10ul of drawing, inject liquid chromatograph, measure, promptly get.
3, precision test
The accurate reference substance solution 10ul that draws repeats sample introduction 5 times, and instrument precision is good as a result, specifically sees the following form.
4, linear relationship
Respectively accurate reference substance solution 2,5,15, the 20ul of drawing injects liquid chromatograph, is ordinate with the common logarithm of peak area, is the mould coordinate with the common logarithm of sample size, the drawing standard curve, regression equation, the result sees the following form.
The result shows: notoginsenoside R 0.300-3.000ug, ginsenoside Rg1 1.010-10.100ug, ginsenoside Rb1 between 1.030-10.300ug, the logarithm value of its logarithm value and peak area is good linear relationship.
5, replica test
Getting same lot sample article prepares by the need testing solution preparation method; Invigorate blood circulation notoginsenoside R in the cream, ginsenoside Rg1, ginsenoside Rb1's content of numerous mountains is respectively: 0.0361mg/ card (n=6; RSD0.9%), 0.4778mg/ card (n=6; RSD=1.5%), 0.3252mg/ card (n=6, RSD1.6%).
The result shows that this law repeatability is good.
6, recovery test
Precision takes by weighing the same a collection of test sample 1.25g of known content, and the accurate reference substance solution 3.0ml that adds presses need testing solution preparation method preparation, measures content, and the result sees the following form.
The result shows: this law recovery is good.
7, sample determination result
The accurate respectively reference substance solution 5 μ l that draw, 10 μ l and need testing solution 10 μ l inject liquid chromatograph, press the external standard two-point method and measure, and measure the result and see the following form.
Sample size is measured result (n=3)
Embodiment
Following examples are done further elaboration to the present invention.
Embodiment 1
The numerous mountains cream method of quality control of invigorating blood circulation
Differentiate: these article 5g is got in (1), adds ethanol 10ml, and sonicated 15 minutes is put coldly, and filtration is got filtrating, as need testing solution.Other gets dragon's blood control medicinal material 0.1g, adds ethanol 5ml and makes dissolving, as control medicinal material solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developping agent with chloroform-methanol (19: 1), launch, take out, dry.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the principal spot of same color.
(2) get these article 10g, add water 70ml, heated and boiled 30 minutes is put coldly, filters; The filtrating 30ml jolting that adds diethyl ether is extracted, and discards ether solution, and water layer extracts 2 times with water saturated normal butyl alcohol jolting, at every turn 40ml; Merge normal butyl alcohol liquid,, discard ammoniacal liquor, again with the saturated water 30ml washing of normal butyl alcohol with ammonia solution 30ml washing; Discard water liquid, normal butyl alcohol liquid evaporate to dryness, residue add methyl alcohol 1ml makes dissolving, as need testing solution; Other gets pseudo-ginseng control medicinal material 1g, adds methyl alcohol 20ml, and refluxing extraction 1 hour is put coldly, filter, and the filtrating evaporate to dryness, residue adds water 20ml makes dissolving, extracts 2 times with water saturated normal butyl alcohol jolting, each 25ml, merging normal butyl alcohol liquid shines medicinal material solution in pairs with legal system; Get ginsenoside Rb1, Rg1 and notoginsenoside R reference substance again, add methyl alcohol and process the mixed solution that every 1ml contains 1mg, as reference substance solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate; With 10 ℃ of lower floor's solution with the held layering of chloroform-methanol-water (13: 7: 2) is developping agent; Launch, take out, dry; Spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the spot colour developing at 105 ℃.Put respectively under daylight and the ultraviolet lamp (365nm) and inspect, in the test sample chromatogram.With control medicinal material and the corresponding position of reference substance chromatogram on, show the spot or the fluorescence spot of same color respectively.
Assay:
The preparation of test sample: get the numerous mountains cream of invigorating blood circulation, remove the lid lining, get that 2.5g is accurate to be claimed surely, put in the apparatus,Soxhlet's; Earlier with extracted by ether 2 hours, after the medicine residue dries, utilize apparatus,Soxhlet's use methanol extraction 4 hours again, methyl alcohol has been carried the liquid evaporate to dryness; Residue water low-grade fever makes dissolving, put cold, last macroporous adsorptive resins (internal diameter 1.5cm, long 10cm); 20% ethanolic solution washing with 2-4 times of column volume discards 20% ethanol eluate, uses 70% ethanol 5-8 times of column volume wash-out again, collects eluent; Water bath method, residue is used dissolve with methanol, with methyl alcohol dilution and constant volume in the 5ml volumetric flask, as need testing solution.
The preparation of reference substance solution: precision takes by weighing that to be dried to ginsenoside Rg1, ginsenoside Rb1, the notoginsenoside R reference substance of constant weight through phosphorus pentoxide an amount of; Add methyl alcohol and process the mixed solution that every 1ml contains ginsenoside Rg1 0.5mg/ml, ginsenoside Rb10.5mg/ml, notoginsenoside R 0.15mg/ml, as reference substance solution.
Chromatographic condition: use octadecylsilane chemically bonded silica to be filling agent; The 5 μ m of Dikma company, 150mm*4.6mm; Moving phase is carried out gradient elution for the acetonitrile-water according to the form below.
It is 120 ℃ that the detecting device drift tube temperature is penetrated in the evaporation color break-up, and flow rate of carrier gas is 2.0L/min.
Measure: accurate respectively reference substance solution 5 μ l, 10 μ l and the need testing solution 10 μ l of drawing, inject liquid chromatograph, measure.
As a result, lot number is that 20100508 the numerous mountains cream content of invigorating blood circulation is: the content of every obedient notoginsenoside R is 0.0387mg, and ginsenoside Rg1's content is 0.4845mg, ginsenoside Rb1 0.3374mg.
Claims (3)
1. the numerous mountains method of quality control of cream of invigorating blood circulation comprises employing: (1) thin-layered chromatography differentiates that the numerous mountains human fat and blood of invigorating blood circulation exhausts middle Dracoalban; (2) thin-layered chromatography is differentiated the numerous mountains ginsenoside Rg1 in the cream pseudo-ginseng that invigorates blood circulation; (3) invigorate blood circulation ginsenoside Rg1 in the cream pseudo-ginseng, ginsenoside Rb1 and notoginsenoside R of high effective liquid chromatography for measuring numerous mountains.
2. the invigorate blood circulation method of quality control of cream of a kind of numerous mountains according to claim 1 is characterized in that: (1) thin-layered chromatography differentiates that the numerous mountains human fat and blood of invigorating blood circulation exhausts middle Dracoalban; (2) thin-layered chromatography is differentiated the numerous mountains ginsenoside Rg1 in the cream pseudo-ginseng that invigorates blood circulation; (3) high performance liquid chromatography-evaporation color break-up is penetrated detection method and is measured the invigorate blood circulation content of ginsenoside Rg1 in the cream pseudo-ginseng, ginsenoside Rb1 and three kinds of compositions of notoginsenoside R of numerous mountains simultaneously.
3. the invigorate blood circulation method of quality control of cream of a kind of numerous mountains according to claim 1 and 2; It is characterized in that: (1) thin-layered chromatography differentiate numerous mountains invigorate blood circulation human fat and blood exhaust in Dracoalban's method be: get these article 5g, add ethanol 10ml, sonicated 15 minutes; Put cold; Filter, get filtrating, as need testing solution.Other gets dragon's blood control medicinal material 0.1g, adds ethanol 5ml and makes dissolving, as control medicinal material solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developping agent with chloroform-methanol (19: 1), launch, take out, dry.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, show the principal spot of same color.
(2) thin-layered chromatography is differentiated and to be invigorated blood circulation numerous mountains ginsenoside Rg1's method is in the cream pseudo-ginseng: get these article 10g, add water 70ml, heated and boiled 30 minutes, put cold, filtration; The filtrating 30ml jolting that adds diethyl ether is extracted, and discards ether solution, and water layer extracts 2 times with water saturated normal butyl alcohol jolting, at every turn 40ml; Merge normal butyl alcohol liquid,, discard ammoniacal liquor, again with the saturated water 30ml washing of normal butyl alcohol with ammonia solution 30ml washing; Discard water liquid, normal butyl alcohol liquid evaporate to dryness, residue add methyl alcohol 1ml makes dissolving, as need testing solution; Other gets pseudo-ginseng control medicinal material 1g, adds methyl alcohol 20ml, and refluxing extraction 1 hour is put coldly, filter, and the filtrating evaporate to dryness, residue adds water 20ml makes dissolving, extracts 2 times with water saturated normal butyl alcohol jolting, each 25ml, merging normal butyl alcohol liquid shines medicinal material solution in pairs with legal system; Get ginsenoside Rb1, Rg1 and notoginsenoside R reference substance again, add methyl alcohol and process the mixed solution that every 1ml contains 1mg, as reference substance solution.According to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate; With 10 ℃ of lower floor's solution with the held layering of chloroform-methanol-water (13: 7: 2) is developping agent; Launch, take out, dry; Spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the spot colour developing at 105 ℃.Put respectively under daylight and the ultraviolet lamp (365nm) and inspect, in the test sample chromatogram.With control medicinal material and the corresponding position of reference substance chromatogram on, show the spot or the fluorescence spot of same color respectively.
(3) high performance liquid chromatography-evaporation color break-up is penetrated detection method and is measured the invigorate blood circulation content of ginsenoside Rg1 in the cream pseudo-ginseng, ginsenoside Rb1 and three kinds of compositions of notoginsenoside R of numerous mountains simultaneously, and condition is:
Chromatographic condition and system suitability test are filling agent with the octadecylsilane chemically bonded silica; With the acetonitrile-water is moving phase, and according to the form below carries out gradient elution:
The evaporation color break-up is penetrated the detecting device condition and is: drift tube temperature is 120 ℃, and flow rate of carrier gas is 2.0L/min; Number of theoretical plate calculates by the ginsenoside Rg1 peak should be not less than 2000.
The preparation of reference substance solution: precision takes by weighing that to be dried to ginsenoside Rg1, ginsenoside Rb1, the notoginsenoside R reference substance of constant weight through phosphorus pentoxide an amount of; Add methyl alcohol and process the mixed solution that every 1ml contains ginsenoside Rg1 0.5mg/ml, ginsenoside Rb10.5mg/ml, notoginsenoside R 0.15mg/ml, as reference substance solution.
The preparation of need testing solution: get the numerous mountains cream of invigorating blood circulation, remove the lid lining, get that 2.5g is accurate to be claimed surely, put in the apparatus,Soxhlet's refluxing extraction that adds diethyl ether 3 hours; Discard ether solution, the dregs of a decoction volatilize, and add methanol eddy and extract 4 hours, and methanol extract liquid evaporate to dryness, residue add the water low-grade fever makes dissolving; Put cold, last macroporous adsorptive resins (internal diameter 1.5cm, long 10cm), 20% the ethanolic solution washing with 2-4 times of column volume discards 20% ethanol eluate; Use 70% ethanol 5-8 times of column volume wash-out again, collect eluent, water bath method, residue is used dissolve with methanol; Be transferred in the 5ml volumetric flask, be diluted to scale, shake up, as need testing solution with methyl alcohol.
Accurate respectively reference substance solution 5 μ l, 10 μ l and the need testing solution 10 μ l of drawing of determination method inject liquid chromatograph, measure, and promptly get.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103017128A CN102809626A (en) | 2012-08-23 | 2012-08-23 | Quality control method of Qianshan Huoxue plaster |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103017128A CN102809626A (en) | 2012-08-23 | 2012-08-23 | Quality control method of Qianshan Huoxue plaster |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102809626A true CN102809626A (en) | 2012-12-05 |
Family
ID=47233386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103017128A Pending CN102809626A (en) | 2012-08-23 | 2012-08-23 | Quality control method of Qianshan Huoxue plaster |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102809626A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259264A (en) * | 2015-10-13 | 2016-01-20 | 哈尔滨市康隆药业有限责任公司 | Method for measuring content of multiple active ingredients in xiaoshuan tongluo tablets |
| CN109781922A (en) * | 2019-03-18 | 2019-05-21 | 青海省药品检验检测院 | A kind of TLC Identification identifying dragon's blood and Resina Draconis |
-
2012
- 2012-08-23 CN CN2012103017128A patent/CN102809626A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 傅欣彤等: "千山活血膏质量标准研究", 《中国中药杂志》 * |
| 宋前流: "薄层色谱与紫外光谱联合鉴别血竭与龙血竭", 《中国药师》 * |
| 张健萍等: "活血止痛散的制备与质量控制", 《中国药房》 * |
| 王雁: "HPLC法测定三七药材中3种皂苷的含量", 《中南药学》 * |
| 闫艳等: "HPLC-ELSD法同时测定心源素胶囊中四种皂苷的含量及其在质量控制中的应用", 《食品科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259264A (en) * | 2015-10-13 | 2016-01-20 | 哈尔滨市康隆药业有限责任公司 | Method for measuring content of multiple active ingredients in xiaoshuan tongluo tablets |
| CN109781922A (en) * | 2019-03-18 | 2019-05-21 | 青海省药品检验检测院 | A kind of TLC Identification identifying dragon's blood and Resina Draconis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103197027A (en) | Quality control method of astragalus-leech capsules capable of regulating collaterals | |
| CN105738546A (en) | Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof | |
| CN105301168B (en) | The detection method of dredging collateral resolving sputum capsule | |
| CN101028388B (en) | Quality inspection of Chinese-medicinal preparation for treating shortsighness and asthenopia | |
| CN101708208B (en) | Detection method of capsule preparation for treating painful swollen joint | |
| CN101966223A (en) | Fingerprint detection method for compound wintercreeper preparation | |
| CN102579734B (en) | Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof | |
| CN104391072A (en) | Quality control method of traditional Chinese medicine compound preparation for treating osteoporosis | |
| CN102269752B (en) | Detection method for pharmaceutical composition preparation | |
| CN103344737A (en) | Quality control method of traditional Chinese medicine tablet for treating nasosinusitis | |
| CN106198810B (en) | A kind of quality determining method of the Chinese medicine composition with treatment tumor chemoradiotherapy bone marrow suppression | |
| CN101204434A (en) | Quality standard for thrombus dispelling pill and test method thereof | |
| CN102879516B (en) | Method for identifying Buyang Huanwu soup and measuring content of Buyang Huanwu soup | |
| CN102218122A (en) | Quality control and detection method for sea dragon and gecko oral liquid | |
| CN101926889B (en) | Method for detecting white paeony root-medlar particles | |
| CN101700262A (en) | Quality control method of andrographis paniculata dropping pills | |
| CN103575850A (en) | Detection method for Gymnadenia conopsea medicinal material | |
| CN102809626A (en) | Quality control method of Qianshan Huoxue plaster | |
| CN101953978B (en) | Heart-soothing and lipid-lowering tablet medicine quality detecting method | |
| CN105616946A (en) | Preparation for treating cough, preparation method and quality control method thereof | |
| CN106918670B (en) | A kind of quality determining method of pharmaceutical composition | |
| CN108414667A (en) | The detection method of one seed ginseng osmanthus Yixin granule quality standard | |
| CN105445385A (en) | A quality detecting method for honeysuckle stem formula granules | |
| CN101632804B (en) | Quality control method for wind-dispelling heat-dissipating capsules | |
| CN103454374A (en) | Quality control method of bone rehabilitation medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121205 |
|
| WD01 | Invention patent application deemed withdrawn after publication |