CN102796516A - L-cysteine fluorescent nano-quantum dot probe and its application - Google Patents
L-cysteine fluorescent nano-quantum dot probe and its application Download PDFInfo
- Publication number
- CN102796516A CN102796516A CN2012102936892A CN201210293689A CN102796516A CN 102796516 A CN102796516 A CN 102796516A CN 2012102936892 A CN2012102936892 A CN 2012102936892A CN 201210293689 A CN201210293689 A CN 201210293689A CN 102796516 A CN102796516 A CN 102796516A
- Authority
- CN
- China
- Prior art keywords
- halfcystine
- quantum dot
- probe
- fluorescence
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses an L-cysteine fluorescent nano-quantum dot probe and its application. The thioglycolic acid modified fluorescent nanometer CdS quantum dot probe provided in the invention is obtained by: in a water bath of 30DEG C, adding a sodium sulfide solution into a mixed solution prepared from thioglycolic acid and a cadmium chloride solution for reaction. When applied in L-cysteine detection, the L-cysteine fluorescent nano-quantum dot probe has high sensitivity and selectivity. The invention has the advantages that: 1. the probe provided in the invention has the characteristics of convenient and simple preparation, easily available raw materials, and low cost; and 2. by using the probe prepared in the invention to conduct L-cysteine detection, the detection process is simple and convenient, the sensitivity is high, the selectivity is good, the detection limit is low, and real-time in-situ online fast detection of L-cysteine in an actual sample can be realized.
Description
Technical field
The invention belongs to biochemical analysis detection technique field, be specifically related to a kind of L-halfcystine fluorescence nano quantum dot probe and application thereof.
Background technology
The L-halfcystine is a kind of amino acid that contains sulfydryl, is a seed amino acid common in the organism, also is to constitute one of many polypeptide and proteinic staple.Because it is active that the sulfydryl in the L-halfcystine molecular structure has very high protophilic, and very easily oxidized, thereby make the L-halfcystine have stronger reductibility.The L-halfcystine plays crucial effect in protein folding, can change between the protein molecule disulfide linkage inner with protein molecule, weakens proteinic structure and protein is stretched.In addition, the L-halfcystine also has oxidation-resistance, detoxification ability and radical scavenging activity, in the various kinds of cell activity, plays an important role.The L-halfcystine lacks can cause a series of syndromes, as: hair bleaching, edema, skin injury, liver damage or the like.Therefore, the detection of L-halfcystine seems particularly important.Up to the present, the detection method about the L-halfcystine has spectrophotometry, fluorescent spectrometry, HPLC, flow injection method, capillary zone electrophoresis method and cyclic voltammetry etc.But these methods common analysis times is long, analytic process is loaded down with trivial details, and sensitivity is lower when being used for actual sample and measuring, selectivity is relatively poor.Set up the L-cysteine detecting method simple, quick, highly sensitive, that selectivity is good and become the focus that people pay close attention to gradually.
The water soluble fluorescence nano-quantum point has caused researchist's in a plurality of research fields extensive concern as a kind of novel fluorescent probe.Compare with GFP with traditional organic fluorescent dye; Quantum dot has very superior spectral quality, as: higher quantum yield, wavelength of fluorescence can be regulated with dimensional change, wide excitation spectrum, narrow and symmetric emmission spectrum, strong anti-photobleaching character and fluorescent stability preferably.These superior spectral qualities are widely used in the biochemical analysis detection quantum dot fluorescence probe.Aspect ion detection, investigators adopt various quantum dot probes to carry out a series of yin, yang ion determination.Aspect biomolecule detection, the quantum dot probe also is used in the analyzing and testing of urea, glucose, medicine and neurotransmitter.These results show the huge applications potentiality of quantum dot fluorescence nano-probe in biochemical analysis field.Up to now, investigators only utilize L-cysteine modified and preparation quantum dot, but do not adopt the quantum dot fluorescence nano-probe directly to detect the L-halfcystine.
To above problem; We have studied a kind of novel method of fluorescence nano quantum dot probe in detecting L-halfcystine; This method is simple to operate, detection is quick, highly sensitive and selectivity is good, can carry out highly sensitive, the highly selective identification of L-halfcystine in the actual sample.
Summary of the invention
The object of the present invention is to provide a kind of fluorescence nano quantum dot probe of the L-of detection halfcystine.
Another object of the present invention is to provide a kind of L-halfcystine fluorescence nano quantum dot probe in sensitivity that detects the L-halfcystine and optionally application.
L-halfcystine fluorescence nano quantum dot probe of the present invention is the fluorescence nano CdS quantum dot that Thiovanic acid is modified.
The technical scheme that the present invention solves the problems of the technologies described above is following:
1.L-halfcystine fluorescence nano quantum dot probe is the fluorescence nano CdS quantum dot that Thiovanic acid is modified.The preparation method is following:
In 30 ℃ water-bath, be to be made into the aqueous solution at 1: 0.05 by the mol ratio of Thiovanic acid and cadmium chloride solution, stir 60min, in solution, add sodium sulfide solution, make S
2-Concentration be 0.005mol/L; Continue to stir 10h; Get final product the fluorescence nano CdS quantum dot modified of Thiovanic acid, under the uv lamp of 365nm, the fluorescence nano CdS quantum dot that Thiovanic acid is modified sends the fluorescence of more weak green; Excite down at the 400nm wavelength, very weak fluorescence emission spectrum is arranged at 450～650nm.
2.L-halfcystine fluorescence nano quantum dot probe is in the application that detects the L-halfcystine.
1) L-halfcystine fluorescence nano quantum dot probe is in the application of the sensitivity that detects the L-halfcystine:
When the fluorescence nano CdS quantum dot that the application Thiovanic acid is modified detects the L-halfcystine as fluorescent probe, the fluorescent nano probe solution after diluting 50 times is mixed with L-halfcystine solution, make that the mol ratio of probe and L-halfcystine is 1: 1～80.Under the uv lamp of 365nm; When having the L-halfcystine to exist; The fluorescence of the green of quantum dot probe strengthens gradually; The fluorescence intensity that can learn fluorescence nano CdS quantum dot probe from fluorescence spectrum figure strengthens with the increase of the concentration of L-halfcystine, and fluorescence intensity and L-semicystinol concentration have good linear relationship, therefore; The fluorescence nano CdS quantum dot that Thiovanic acid is modified can be used as the content of the probe L-halfcystine fast and in the detection by quantitative aqueous solution that detects the L-halfcystine, can reach 3.8nM to the detectability of L-halfcystine;
2) L-halfcystine fluorescence nano quantum dot probe is in the optionally application that detects the L-halfcystine:
When the fluorescence nano CdS quantum dot that the application Thiovanic acid is modified detects the L-halfcystine as fluorescent probe, under the same terms, common different kinds of ions: K
+, NH
4 +, Ca
2+, Mg
2+, Al
3+, Fe
2+, Mn
2+, NO
3 -, C
2O
4 2-, SO
4 2-, Cl
-All can not influence the detection of L-halfcystine, the detection that all the other amino acid, common carbohydrate and base are not all disturbed the L-halfcystine has higher selectivity.Therefore, the fluorescence nano CdS quantum dot probe of Thiovanic acid modification can be applicable to the real-time in-situ detection of L-halfcystine in the actual biological sample.
Advantage of the present invention:
1. that fluorescence nano CdS quantum dot probe provided by the invention prepares is convenient and simple, raw material is easy to get, and cost is low.
2. adopt the prepared probe of the present invention to carry out the detection of L-halfcystine, testing process is simple and convenient, and is highly sensitive, selectivity good, detectability is low, can realize the real-time in-situ on-line quick detection of L-halfcystine in the actual sample.
Description of drawings
Fig. 1 is in the fluorescence nano CdS quantum dot probe in detecting L-halfcystine process of Thiovanic acid modification of the present invention; Behind the fluorescence nano CdS quantum dot probe reaction that Different L-semicystinol concentration and Thiovanic acid are modified, resulting fluorescence spectrum figure when excitation wavelength is 400nm;
Among the figure, the Different L-semicystinol concentration of a ~ m for adding among the embodiment, the mol ratio that makes probe and L-halfcystine is 1: 1～80 fluorescence spectrum figure.
Embodiment
For a better understanding of the present invention, below in conjunction with concrete embodiment the present invention is done further elaboration in detail.Should be understood that following examples are preferred implementations more of the present invention, purpose is to set forth better content of the present invention, rather than protection scope of the present invention is produced any restriction.
Embodiment 1
The preparation of the fluorescence nano CdS quantum dot that Thiovanic acid is modified:
In 30 ℃ water-bath, be to be made into the aqueous solution at 1: 0.05 by the mol ratio of Thiovanic acid and cadmium chloride solution, stir 60min, in solution, add sodium sulfide solution, make S
2Concentration be 0.005mol/L; Continue to stir 10h; Get final product the fluorescence nano CdS quantum dot modified of Thiovanic acid, under the uv lamp of 365nm, the fluorescence nano CdS quantum dot that Thiovanic acid is modified sends the fluorescence of more weak green; Excite down at the 400nm wavelength, very weak fluorescence emission spectrum is arranged at 480～650nm.
Embodiment 2
The fluorescence nano CdS quantum dot probe that Thiovanic acid is modified is in the application of the sensitivity that detects the L-halfcystine:
1. the fluorescence nano CdS quantum dot of using the Thiovanic acid modification detects in the process of L-halfcystine in children's's amino acid injection as fluorescent probe; Fluorescent nano probe solution after diluting 50 times is mixed with L-halfcystine solution; Make that the mol ratio of probe and L-halfcystine is 1: 1, promptly obtain fluorescence spectrum a.
2. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 2 into, then obtains fluorescence spectrum b;
3. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 4 into, then obtains fluorescence spectrum c;
4. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 6 into, then obtains fluorescence spectrum d;
5. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 8 into, then obtains fluorescence spectrum e;
6. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 10 into, then obtains fluorescence spectrum f;
7. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 12 into, then obtains fluorescence spectrum g;
8. all the other conditions are all with last identical, and only the mol ratio of probe and L-halfcystine changes 1: 14 into, then obtains fluorescence spectrum h;
9. all the other conditions are all with last identical,, only the mol ratio of probe and L-halfcystine changes 1: 17 into, then obtains fluorescence spectrum i;
10. all the other conditions are all with last identical,, only the mol ratio of probe and L-halfcystine changes 1: 20 into, then obtains fluorescence spectrum j;
11. all the other conditions are all with last identical,, only the mol ratio of probe and L-halfcystine changes 1: 40 into, then obtains fluorescence spectrum k;
12. all the other conditions are all with last identical,, only the mol ratio of probe and L-halfcystine changes 1: 60 into, then obtains fluorescence spectrum l;
13. all the other conditions are all with last identical,, only the mol ratio of probe and L-halfcystine changes 1: 80 into, then obtains fluorescence spectrum m;
Under the uv lamp of 365nm, when having the L-halfcystine to exist, the fluorescence of the green of quantum dot probe strengthens gradually.The fluorescence intensity that can learn fluorescence nano CdS quantum dot probe from fluorescence spectrum figure strengthens with the increase of the concentration of L-halfcystine, and the increased value of fluorescence intensity and L-semicystinol concentration have good linear relationship, R
2=0.9969.
Embodiment 3
The fluorescence nano CdS quantum dot probe that Thiovanic acid is modified is in the optionally application that detects the L-halfcystine:
The fluorescence nano CdS quantum dot of using the Thiovanic acid modification detects in the process of L-halfcystine in children's's amino acid injection as fluorescent probe; Fluorescent nano probe solution after diluting 50 times is mixed with L-halfcystine solution; Make that the mol ratio of probe and L-halfcystine is 1: 1; The fluorescence intensity level of gained and the fluorescence intensity level of the gained behind the adding coexisting substances are compared, and the result is as shown in the table.
The interference of coexisting substances
Claims (2)
1.L-halfcystine fluorescence nano quantum dot probe is the fluorescence nano CdS quantum dot that Thiovanic acid is modified, and it is characterized in that the preparation method is following:
In 30 ℃ water-bath, be to be made into the aqueous solution at 1: 0.05 by the mol ratio of Thiovanic acid and cadmium chloride solution, stir 60min, in solution, add sodium sulfide solution, make S
2-Concentration be 0.005mol/L; Continue to stir 10h; Get final product the fluorescence nano CdS quantum dot modified of Thiovanic acid, under the uv lamp of 365nm, the fluorescence nano CdS quantum dot that Thiovanic acid is modified sends the fluorescence of more weak green; Excite down at the 400nm wavelength, very weak fluorescence emission spectrum is arranged at 450～650nm.
2.L-the application of halfcystine fluorescence nano quantum dot probe is characterized in that, in the application that detects the L-halfcystine;
1) L-halfcystine fluorescence nano quantum dot probe is in the application of the sensitivity that detects the L-halfcystine:
When the fluorescence nano CdS quantum dot that the application Thiovanic acid is modified detects the L-halfcystine as fluorescent probe; Fluorescent nano probe solution after diluting 50 times is mixed with L-halfcystine solution; Make that the mol ratio of probe and L-halfcystine is 1: 1～80, under the uv lamp of 365nm, when having the L-halfcystine to exist; The fluorescence of the green of quantum dot probe strengthens gradually; The fluorescence intensity that can learn fluorescence nano CdS quantum dot probe from fluorescence spectrum figure strengthens with the increase of the concentration of L-halfcystine, and fluorescence intensity and L-semicystinol concentration have good linear relationship, therefore; The fluorescence nano CdS quantum dot that Thiovanic acid is modified can be used as the content of the probe L-halfcystine fast and in the detection by quantitative aqueous solution that detects the L-halfcystine, can reach 3.8nM to the detectability of L-halfcystine;
2) L-halfcystine fluorescence nano quantum dot probe is optionally used at detection L-halfcystine:
When the fluorescence nano CdS quantum dot that the application Thiovanic acid is modified detects the L-halfcystine as fluorescent probe, under the same terms, common different kinds of ions: K
+, NH
4 +, Ca
2+, Mg
2+, Al
3+, Fe
2+, Mn
2+, NO
3 -, C
2O
4 2-, SO
4 2-, Cl
-All can not influence the detection of L-halfcystine; The detection that all the other amino acid, common carbohydrate and base are not all disturbed the L-halfcystine; Has higher selectivity; Therefore, the fluorescence nano CdS quantum dot probe of Thiovanic acid modification can be applicable to the real-time in-situ detection of L-halfcystine in the actual biological sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102936892A CN102796516A (en) | 2012-06-11 | 2012-08-17 | L-cysteine fluorescent nano-quantum dot probe and its application |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210190562 | 2012-06-11 | ||
| CN201210190562.8 | 2012-06-11 | ||
| CN2012102936892A CN102796516A (en) | 2012-06-11 | 2012-08-17 | L-cysteine fluorescent nano-quantum dot probe and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102796516A true CN102796516A (en) | 2012-11-28 |
Family
ID=47195828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102936892A Pending CN102796516A (en) | 2012-06-11 | 2012-08-17 | L-cysteine fluorescent nano-quantum dot probe and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102796516A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217406A (en) * | 2013-03-21 | 2013-07-24 | 上海交通大学 | Making method of Au/Ag core/shell quantum dot-based cysteine-Cu2<+> fluorescent probe |
| CN105044057A (en) * | 2015-07-06 | 2015-11-11 | 广西师范学院 | Method for detecting concentration of L-cysteine by using graphene quantum dot and nano-gold |
| CN110715914A (en) * | 2019-11-05 | 2020-01-21 | 鲁东大学 | Multi-mode detection method of L-cysteine |
| CN111826155A (en) * | 2020-07-23 | 2020-10-27 | 安徽大学 | CdS quantum dot-fluorescein FRET fluorescent probe and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225300A (en) * | 2008-02-14 | 2008-07-23 | 华东师范大学 | Preparation method of size-adjustable cds quantum dot |
-
2012
- 2012-08-17 CN CN2012102936892A patent/CN102796516A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225300A (en) * | 2008-02-14 | 2008-07-23 | 华东师范大学 | Preparation method of size-adjustable cds quantum dot |
Non-Patent Citations (3)
| Title |
|---|
| SHAN HUANG ET AL: "A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots", 《ANALYTICA CHIMICA ACTA》, vol. 645, 3 May 2009 (2009-05-03), pages 73 - 78 * |
| 张震雷等: "CdS纳米微粒的聚四氟乙烯多孔膜法制备及其表面修饰", 《应用化学》, vol. 21, no. 8, 31 August 2004 (2004-08-31) * |
| 蒋茶等: "CdS量子点的制备及细胞膜初步荧光标记", 《分析实验室》, vol. 26, 31 May 2007 (2007-05-31), pages 1 - 4 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217406A (en) * | 2013-03-21 | 2013-07-24 | 上海交通大学 | Making method of Au/Ag core/shell quantum dot-based cysteine-Cu2<+> fluorescent probe |
| CN103217406B (en) * | 2013-03-21 | 2015-09-09 | 上海交通大学 | Based on halfcystine and the Cu of Au/Ag core/shell quantum dot 2+the method for making of fluorescence probe |
| CN105044057A (en) * | 2015-07-06 | 2015-11-11 | 广西师范学院 | Method for detecting concentration of L-cysteine by using graphene quantum dot and nano-gold |
| CN105044057B (en) * | 2015-07-06 | 2017-11-24 | 广西师范学院 | A kind of method for detecting L semicystinol concentrations using graphene quantum dot and nanogold |
| CN110715914A (en) * | 2019-11-05 | 2020-01-21 | 鲁东大学 | Multi-mode detection method of L-cysteine |
| CN110715914B (en) * | 2019-11-05 | 2022-07-12 | 鲁东大学 | Multi-mode detection method of L-cysteine |
| CN111826155A (en) * | 2020-07-23 | 2020-10-27 | 安徽大学 | CdS quantum dot-fluorescein FRET fluorescent probe and preparation method and application thereof |
| CN111826155B (en) * | 2020-07-23 | 2022-06-07 | 安徽大学 | CdS quantum dot-fluorescein FRET fluorescent probe and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103217406B (en) | Based on halfcystine and the Cu of Au/Ag core/shell quantum dot 2+the method for making of fluorescence probe | |
| Liu et al. | A novel and convenient near-infrared fluorescence “turn off–on” nanosensor for detection of glucose and fluoride anions | |
| Huang et al. | A dual-channel fluorescent chemosensor for discriminative detection of glutathione based on functionalized carbon quantum dots | |
| Zeng et al. | Determination of melamine by flow injection analysis based on chemiluminescence system | |
| Li et al. | Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization coupled with capillary electrophoresis separation | |
| Fu et al. | Rational design of an “on-off-on” fluorescent assay for chiral amino acids based on quantum dots and nanoporphyrin | |
| CN108003869B (en) | Fluorescent probe for detecting hypochlorite with high sensitivity and synthesis method and application thereof | |
| CN108129459B (en) | Novel fluorescent probe for detecting sulfur dioxide and application thereof | |
| Feng et al. | Silver nanoclusters with enhanced fluorescence and specific ion recognition capability triggered by alcohol solvents: a highly selective fluorimetric strategy for detecting iodide ions in urine | |
| CN102796516A (en) | L-cysteine fluorescent nano-quantum dot probe and its application | |
| CN105319192A (en) | Method for detecting hypochlorite anions through water-soluble fluorescent silica nanoparticle | |
| CN105954345A (en) | Method for electrochemically determining content of vitamins in fruit juice | |
| Wang et al. | An effective method towards label-free detection of antibiotics by surface-enhanced Raman spectroscopy in human serum | |
| CN107814808A (en) | A kind of near-infrared response type two-photon fluorescence probe and its preparation method and application | |
| CN103439269A (en) | Method for quickly detecting physicochemical indexes in fermented grains by near infrared | |
| CN106468681A (en) | A kind of selective light electrochemical analysis method of Microcystins in Water MC-LR | |
| CN107253961A (en) | It is a kind of can ratio test cysteine water soluble fluorescence sensor preparation and application | |
| CN113292582A (en) | Synthesis and application of bifunctional fluorescent probe capable of distinguishing hydroxyl free radicals and hydrogen peroxide simultaneously | |
| Geană et al. | Discrimination and classification of wines based on polypyrrole modified screen-printed carbon electrodes coupled with multivariate data analysis | |
| Liang et al. | A camphor-based fluorescent probe with high selectivity and sensitivity for formaldehyde detection in real food samples and living zebrafish | |
| CN103149184A (en) | Fluorescence detection method for hypochlorite | |
| Hendy et al. | A spectrophotometric and NMR study on the formation of an inclusion complex between dopamine and a sulfonated cyclodextrin host | |
| CN110186886A (en) | The inversion method of Microcystins in Water MC-LR concentration | |
| CN105021580A (en) | 17beta-estradiol detection method | |
| CN110412000B (en) | Fluorescent probe for detecting L-tryptophan based on ten-element cucurbituril and detection method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121128 |