CN102793657A - Skin care product containing biological buffering agent - Google Patents
Skin care product containing biological buffering agent Download PDFInfo
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- CN102793657A CN102793657A CN2012103026201A CN201210302620A CN102793657A CN 102793657 A CN102793657 A CN 102793657A CN 2012103026201 A CN2012103026201 A CN 2012103026201A CN 201210302620 A CN201210302620 A CN 201210302620A CN 102793657 A CN102793657 A CN 102793657A
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Abstract
The invention relates to a skin care product containing a biological buffering agent. The product includes microbial fermentation product extracts, and is characterized by further including the biological buffering agent with a mass percentage of 0.1-3.0%. The skin care product has the advantages that: the pH value of the product drifts in a small amplitude, the pH value stabilizes at 6.0-8.0 in three years after preparation of the skin care product, good protection is applied on the microbial fermentation product extracts added in the product, the microbial fermentation product extracts are not easy to be hydrolyzed, and anti-aging effects of the product lasts a long time.
Description
Technical field
The present invention relates to a kind of skin care item that contain biological buffer.
Background technology
With development of science and technology, used the increasing active component that obtains through microbial fermentation engineering in the cosmetic formulations, it has comprised without cell wall breaking handles, handles the active component that obtains through cell wall breaking, general designation microbial fermentation product extract.For example the use of culture propagation product extract in cosmetics is frequent, and its effect is also extensively approved.Aspect the opposing skin aging, culture propagation product extract synthesizes with collagenic protein Skin Cell regeneration all has good facilitation; In addition, culture propagation product extract can also promote the elastin hypertrophy, accelerates the metabolism of skin corium, reduces signs of skin aging, the damaged skin function of releiving, and skin-friendly is healthy.Except that yeast, much other microbial fermentation product extract like Pseudoalteromonas, thermus thermophilus, lactobacillus, aspergillosis, bacillus cereus or the like, also possesses good skin-protecting function to skin.These microbial fermentation product extracts, the main component that skin nursing is played effect is polypeptide and nucleic acid material.Polypeptide and nucleic acid material to the damage that Skin Cell causes, have fine opposing and protective effect to heat, free radical, heavy metal and toxin etc.But also have following problem: when polypeptide and nucleic acid material add in the cosmetics as functional component, they are easy to inactivation, and reason is that this type bioactive ingredients needs stable p H environment usually, generally is that requirement pH is stabilized in 6.0-8.0.The common shelf-life of existing skin care item is 3 years; Contain various Organic substances in the prescription; These Organic substances are in storage process; Because caused the pH value of product to float to below 6.0 by eremacausis, the active substance that the drift of pH value causes adding in the microbial fermentation product extract wherein is hydrolyzed and inactivation.Therefore; Existing skin care item need a kind of to human body safety, nontoxic, the compatible good buffer composition of physiology; The pH value that this composition can be kept product in 3 year shelf-life at cosmetics is at 6.0-8.0, to solve the technical barrier that microbial fermentation product extract that product pH drift causes adding skin care item loses effect.
Summary of the invention
The technical problem that the present invention will solve is; Provide a kind of and can reduce skin care item pH value drift amplitude; Make the product pH value can be stabilized in the skin care item that contain biological buffer of 6.0-8.0 in back 3 years in preparation, to add wherein the microbial fermentation product extract have the advantage of better protection.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of skin care item that contain biological buffer, comprise the microbial fermentation product extract, and it is characterized in that: also comprise the biological buffer that mass percent is 0.1%-3.0%.
Further; Said biological buffer is selected from piperazine-1; 4-two ethyl sulfonic acids, N-carbamoyl methyl ethyl sulfonic acid, 3-(N-morpholinyl)-2-hydroxy-propanesulfonic acid, 1; 3-two [three (methylol) methylamino] propane, N, a kind of in two (2-the ethoxy)-2-aminoethyl sulfonic acid of N-, 3-morpholine propane sulfonic acid, N-three (methylol) methyl-2-aminoethyl sulfonic acid, 4-HEPES, 3-[two (2-ethoxy) amino of N-N-]-2-hydroxy-propanesulfonic acid, 3-Pehanorm-2-hydroxy-propanesulfonic acid, 3-(the hydroxyethyl piperazine)-2-hydroxy-propanesulfonic acid or their mixture.
As of the present invention preferred, said biological buffer is the 4-HEPES, and the mass percent in skin care item is 1%.The cas number of 4-HEPES is 7365-45-9, molecular formula: C8H18N2O4S, molecular weight: 238.31.
In the applied chemistry; Common buffer system is the citric acid-sodium citrate buffer system, acetic acid-sodium acetate buffer system, potassium dihydrogen phosphate and dipotassium hydrogen phosphate (or sodium dihydrogen phosphate and sodium hydrogen phosphate) buffer system etc.; But citric acid-sodium citrate system pH buffering range is between 2.5-5.6; Acetic acid-sodium acetate buffer system pH buffering range is between 3.7-5.6, and potassium dihydrogen phosphate and dipotassium hydrogen phosphate pH buffer system buffering range are between 5.8-8.0, so sodium citrate-citric acid buffer system; Acetic acid-sodium acetate buffer system all is not suitable for providing microbial cell to dissolve the requirement that born of the same parents' thing extract keeps active pH 6.0-8.0; And potassium dihydrogen phosphate and dipotassium hydrogen phosphate buffer system, phosphate radical meeting wherein and the bivalent cation in the microbial fermentation product extract form deposition, also are not suitable for using.
Advantage of the present invention is that the pH scope of using biological buffer adjustment skin care item is between the 6.0-8.0.The special 4-HEPES of selecting, because it is a microorganism culturing biological buffer commonly used, its pH buffering range is 6.8-8.2, the discord bivalent cation combines; PKa in the time of its 25 ℃ is 7.48, is particularly suitable for the microbial fermentation product extract and keeps active, and to human body safety; Nontoxic, the physiology compatibility is good, and chemical property is very stable; Price is also lower in various biological buffer, joins in the skin care item prescription, can be in 3 year shelf-life; Product pH value stable maintenance between 6.0-8.0, can be kept the activity of microbial fermentation product extract preferably, carry the stability and the half-life of polypeptide composition in the product.
Experiment showed, to contain 1% 4-HEPES 10% culture propagation product extract; 0.088% potassium hydroxide uses 0.2% methyl hydroxybenzoate to be antiseptic, and all the other are the compositions of water; Mix homogeneously, its original ph are 7.00, preserve at normal temperatures after 3 years and measure; Its pH value is 6.83, has proved that buffer system possesses very outstanding long-term buffer capacity.The test of use capillary electrophoresis; Contain 1%4-HEPES and 10% culture propagation product extract, 0.088% potassium hydroxide (90%), 0.2% methyl hydroxybenzoate are antiseptic; All the other are the compositions of water, preserve that its polypeptide total content is 94.5% of a former content after 3 years; And do not contain the 1%4-HEPES, only contain 10% culture propagation product extract, 0.012% potassium hydroxide (90%); 0.2% methyl hydroxybenzoate, surplus is the compositions of water, original ph is all 7.0; Preserve that its pH value has floated to 4.1 after 3 years, its polypeptide total content only residual 16.5%.
In sum; The present invention has following advantage: product pH value drift amplitude is little; In 3 years after skin care item preparations, its pH value can be stabilized in 6.0-8.0, to add wherein the microbial fermentation product extract good protection is arranged; The microbial fermentation product extract is not easy to be hydrolyzed, product senile-resistant efficacy longer duration.Skin care item of the present invention include but not limited to skin-care cosmetics such as surfactant, protective skin cream, emulsion essence.
The specific embodiment
Embodiment one: the cosmetic water of preserving moisture that contains biological buffer 4-HEPES
Preparation technology:
1. main pot: the A1-A6 component is stirred, be heated to 65 ℃, be incubated 10 minutes, be stirred to dissolving fully, be cooled to 38 ℃;
2. premixing pot: be pre-mixed B1, B2 component, be stirred to add after the dissolving fully and become owner of pot, stir;
3. the C1 component is added and become owner of pot, must contain the cosmetic water of preserving moisture of biological buffer 4-HEPES after stirring.
PH value when this embodiment prepares and accomplishes is 7.05, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.81, preserves that its polypeptide total content is 94.2% of a former content after 3 years.
Embodiment two: the nutrition essence that contains biological buffer 4-HEPES
Preparation technology:
1. main pot: the A1-A6 component is stirred, be heated to 75 ℃, be stirred to dissolving fully;
2. oil phase pot: drop into the oil phase pot to the B1-B3 component, be heated to 75 ℃, be stirred to dissolving fully;
3. main pot: pour material in the oil phase pot into main pot, holding temperature is at 75 ℃, 3000rpm, and homogenizing 30 minutes cools to 45 ℃ then;
4. main pot: drop into the C1 component in the main pot, holding temperature is at 45 ℃, 3000rpm, and homogenizing 10 minutes cools to 38 ℃ then;
5. premixing pot: be pre-mixed stirring and dissolving to D1, D2, D3 component;
6. D1, D2, the D3 component of mix homogeneously added and become owner of pot, stir;
7. add the E1 component and become owner of pot, stir, be cooled to 33 ℃, must contain the nutrition essence of biological buffer 4-HEPES.
PH value was 7.05 when the preparation of this example was accomplished, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.89, preserved that its polypeptide total content is 96.7% of a former content after 3 years.
Embodiment three: the whitening emulsion that contains biological buffer 4-HEPES
Preparation technology:
1. main pot: the A1-A8 component is mixed, stirred, be heated to 75 ℃, be stirred to dissolving fully;
2. oil phase pot: drop into the oil phase pot to the B1-B9 component, be heated to 75 ℃, be stirred to dissolving fully;
3. main pot: pour material in the oil phase pot into main pot, holding temperature is at 75 ℃, 3000rpm, and homogenizing 30 minutes cools to 45 ℃ then.
4. main pot: input C1 component in main pot, holding temperature be at 45 ℃, 3000rpm, and homogenizing 10 minutes cools to 38 ℃ then.
5. premixing pot: be pre-mixed stirring and dissolving to D1, D2, D3 component;
6. add the D1 of mix homogeneously, D2, D3 component and become owner of pot, stir;
7. add E1, E2 component and become owner of pot, stir, be cooled to 33 ℃, must contain the whitening emulsion of biological buffer 4-hydroxyethyl piperazine ethanetricarboxylic acid.
PH value was 7.07 when this embodiment prepared and accomplishes, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.03, preserved that its polypeptide total content is 90.2% of a former content after 3 years.
Embodiment 4: contain the anti-ageing defrost of preserving moisture of biological buffer 4-HEPES
Preparation technology:
1. main pot: the A1-A8 component is mixed, stirred, be heated to 75 ℃, be stirred to dissolving fully;
2. oil phase pot: drop into the oil phase pot to the B1-B11 component, be heated to 75 ℃, be stirred to dissolving fully;
3. main pot: pour material in the oil phase pot into main pot, holding temperature is at 75 ℃, 3000rpm, and homogenizing 30 minutes cools to 45 ℃ then.
4. main pot: in main pot, drop into the C1 component, holding temperature is at 45 ℃, and high speed homogenization 10 minutes cools to 38 ℃ then.
5. premixing pot: be pre-mixed D1, D2 component, be stirred to dissolving fully;
6. add the D1 of mix homogeneously, D2 component and become owner of pot, stir;
7. add the E1-E4 component and become owner of pot, stir; The F1-F4 component added become owner of pot, stir, be cooled to 33 ℃, must contain the anti-ageing defrost of preserving moisture of biological buffer 4-HEPES.
PH value was 7.12 when this embodiment prepared and accomplishes, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.96, preserved that its polypeptide total content is 93.2% of a former content after 3 years.
Embodiment five: the Shu Min essence that contains biological buffer 3-Pehanorm-2-hydroxy-propanesulfonic acid
Preparation technology:
1. main pot: the A1-A7 component is mixed, stirred, be heated to 75 ℃, be stirred to dissolving fully;
2. oil phase pot: drop into the oil phase pot to the B1-B3 component, be heated to 75 ℃, be stirred to dissolving fully;
3. main pot: pour material in the oil phase pot into main pot, holding temperature is at 75 ℃, 3000rpm, and homogenizing 30 minutes cools to 45 ℃ then.
4. main pot: input C1 component in main pot, holding temperature be at 45 ℃, 3000rpm, and homogenizing 10 minutes cools to 38 ℃ then.
5. premixing pot: be pre-mixed D1, D2, D3 component, be stirred to dissolving fully;
6. add the D1 of mix homogeneously, D2, D3 component and become owner of pot, stir;
7. add E1, E2 component and become owner of pot, stir, be cooled to 33 ℃, must contain the Shu Min essence of biological buffer 3-Pehanorm-2-hydroxy-propanesulfonic acid.
PH value was 7.11 when this embodiment prepared and accomplishes, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.98, preserved that its polypeptide total content is 91.7% of a former content after 3 years.
Annotate: biological buffer 3-Pehanorm-2-hydroxy-propanesulfonic acid in the present embodiment, the pKa in the time of 25 ℃ is 7.61.
Embodiment 6: the moisturiser that contains biological buffer N-carbamoyl methyl ethyl sulfonic acid
Preparation technology:
1. main pot: the A1-A8 component is stirred, be heated to 75 ℃, be stirred to dissolving fully;
2. oil phase pot: drop into the oil phase pot to the B1-B11 component, be heated to 75 ℃, be stirred to dissolving fully;
3. main pot: pour material in the oil phase pot into main pot, holding temperature is at 75 ℃, 3000rpm, and homogenizing 30 minutes cools to 45 ℃ then;
4. main pot: drop into the C1 component in the main pot, holding temperature is at 45 ℃, 3000rpm, and homogenizing 10 minutes cools to 38 ℃;
5. premixing pot: be pre-mixed D1, D2 component, be stirred to dissolving fully;
6. add the D1, the D2 component that stir and become owner of pot, stir;
7. add the E1 component and become owner of pot, stir, add the F1 component, after stirring, be cooled to 33 ℃, must contain the moisturiser of biological buffer N-carbamoyl methyl ethyl sulfonic acid.
PH value was 6.85 when this embodiment prepared and accomplishes, and after keeping in Dark Place at normal temperatures 3 years, measuring pH value is 6.63, preserved that its polypeptide total content is 92.1% of a former content after 3 years.
Annotate: N-carbamoyl methyl ethyl sulfonic acid in the present embodiment, the pKa in the time of 25 ℃ is 6.78.
Claims (3)
1. skin care item that contain biological buffer comprise the microbial fermentation product extract, it is characterized in that: also comprise the biological buffer that mass percent is 0.1%-3.0%.
2. the skin care item that contain biological buffer according to claim 1; It is characterized in that: said biological buffer is selected from piperazine-1; 4-two ethyl sulfonic acids, N-carbamoyl methyl ethyl sulfonic acid, 3-(N-morpholinyl)-2-hydroxy-propanesulfonic acid, 1; 3-two [three (methylol) methylamino] propane, N, a kind of in two (2-the ethoxy)-2-aminoethyl sulfonic acid of N-, 3-morpholine propane sulfonic acid, N-three (methylol) methyl-2-aminoethyl sulfonic acid, 4-HEPES, 3-[two (2-ethoxy) amino of N-N-]-2-hydroxy-propanesulfonic acid, 3-Pehanorm-2-hydroxy-propanesulfonic acid, 3-(the hydroxyethyl piperazine)-2-hydroxy-propanesulfonic acid or their mixture.
3. the skin care item that contain biological buffer according to claim 2 is characterized in that: said biological buffer is the 4-HEPES, and the mass percent in skin care item is 1%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210302620.1A CN102793657B (en) | 2012-08-22 | 2012-08-22 | A kind of skin care item containing biological buffer |
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| CN201210302620.1A CN102793657B (en) | 2012-08-22 | 2012-08-22 | A kind of skin care item containing biological buffer |
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| CN102793657A true CN102793657A (en) | 2012-11-28 |
| CN102793657B CN102793657B (en) | 2015-09-16 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380816A (en) * | 2015-12-14 | 2016-03-09 | 北京肽康生物科技有限公司 | Use of bioactive peptide in preparation of cosmetics |
| CN105434300A (en) * | 2015-03-17 | 2016-03-30 | 欧诗漫生物股份有限公司 | Whitening cream containing pearl powder |
| CN111727034A (en) * | 2017-09-15 | 2020-09-29 | 株式会社爱茉莉太平洋 | Composition for skin whitening comprising culture of pseudoalteromonas peptaiytica or extract thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1501903A (en) * | 2001-05-09 | 2004-06-02 | ��Ԩ��ѧ��ҵ��ʽ���� | Stable solution of reduced coenzyme q |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
-
2012
- 2012-08-22 CN CN201210302620.1A patent/CN102793657B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1501903A (en) * | 2001-05-09 | 2004-06-02 | ��Ԩ��ѧ��ҵ��ʽ���� | Stable solution of reduced coenzyme q |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434300A (en) * | 2015-03-17 | 2016-03-30 | 欧诗漫生物股份有限公司 | Whitening cream containing pearl powder |
| CN105434300B (en) * | 2015-03-17 | 2018-10-12 | 欧诗漫生物股份有限公司 | A kind of skin whitener containing pearl powder |
| CN105380816A (en) * | 2015-12-14 | 2016-03-09 | 北京肽康生物科技有限公司 | Use of bioactive peptide in preparation of cosmetics |
| CN111727034A (en) * | 2017-09-15 | 2020-09-29 | 株式会社爱茉莉太平洋 | Composition for skin whitening comprising culture of pseudoalteromonas peptaiytica or extract thereof |
| CN111727034B (en) * | 2017-09-15 | 2023-02-24 | 株式会社爱茉莉太平洋 | Composition for skin whitening comprising culture of pseudoalteromonas peptaiytica or extract thereof |
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| CN102793657B (en) | 2015-09-16 |
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Address after: Hangzhou City, Zhejiang province 310012 Xihu District World Trade Center Building 1, and Richland city (area D) room 1605 Applicant after: Proya Cosmetics Co., Ltd. Address before: 313023 No. 16, National Road, Huzhou Industrial Park, Zhejiang Province Applicant before: proya (Huzhou) Cosmetics Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: PROYA (HUZHOU) COSMETIC CO., LTD. TO: PROYA COSMETICS CO., LTD. |
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