CN102782493A

CN102782493A - Filter container for collecting cells

Info

Publication number: CN102782493A
Application number: CN2011800118633A
Authority: CN
Inventors: 金铉昌
Original assignee: PATHTECH CO Ltd
Current assignee: PATHTECH CO Ltd
Priority date: 2010-10-19
Filing date: 2011-05-06
Publication date: 2012-11-14
Also published as: KR101019085B1; WO2012053712A1

Abstract

The present invention comprises an attachable/detachable coupled structure including an upper portion and a lower portion, which can be separated and assembled, and which can easily separate cells and foreign substances contained in a solution for preserving cells using a membrane member and a filtering member, and easily collect the separated cells on a slide.

Description

The filter container that is used for collecting cell

Technical field

The present invention relates to be used for the filter container of collecting cell; And more particularly; Relate to the filter container that is used for collecting cell; Said filter container has improved structure, and said structure comprises: upper container and following container, and said upper container and said time container are being connected by attached/removably; And diaphragm element and filter member, said diaphragm element has different size of meshes with said filter member, preserves the cell that is contained in the liquid with easy separation.

Background technology

In general, no abnormal in order to have had a physical examination, collect corresponding to cell, and collected cell be kept in the solution container that contains cell-preservation liquid, and then the part of the health of inspection, with cell attachment on microslide and use microscope inspection.

According to prior art; In addition solution container is provided so that the cell that will adhere on the brush is distributed in the liquid; And the agitation of solutions container is so that cell fully is distributed in the liquid; And liquid is poured in another container, makes liquid use the absorption apparatus closely to adhere under the filtrator and be adsorbed, and brushed on the microslide so that it is collected through the cell of filtrator filtration through adsorb apparatus.

Through container and body portion are left so that make the collecting layer near microslide, remaining mixture solution is gushed out downwards because of action of gravity and therefore disappearance in the container.

Yet, because the user must manually move to microslide from solution container with cell, thus the plenty of time spent, and pollutant or impurity possibly transfer to microslide from user's hand, and this can reduce the accuracy of cytoscopy.

In addition, in solution container, possibly contain the foreign matter except that collected cell, and in the case, be difficult to separate foreign matter and cell, and therefore need the additional separation instrument.

Summary of the invention

Technical matters

Target of the present invention provides a kind of filter container that is used for collecting cell, and said filter container has improved structure, and said structure is used for the collected cell of easy separation and preserves liquid, and wherein can collect institute's isolated cell easily.

Technical solution

According to aspects of the present invention; Provide: the upper container body; It comprises have the accomodating unit of opening so that holding the top of the solution that contains cell, the insertion unit that is arranged under the said accomodating unit and opens downwards, and through being provided with to divide accomodating unit and the diaphragm element that inserts the unit; And following vessel; It comprises having and removably connects being contained in the filter unit of the top of inserting in the unit and the upper surface of admitting the cell through said diaphragm element, and is arranged under the said filter unit and has the exhaust unit in the space that is used to discharge the solution through said filter unit.

Advantageous effects

According to the present invention, a kind of filter container that is used for collecting cell has improved structure, wherein, but the cell and the foreign matter that are contained in the easy separation cell-preservation liquid, and collect institute's isolated cell easily.According to the present invention; Said filter container comprises top and lower part; Said top and said lower part are can be attached/structure that removably is connected; Said structure can be separated or assemble, and the cell that is contained in the cell-preservation liquid and foreign matter can use diaphragm element and filter member to separate easily, and institute's isolated cell can be collected on the microslide easily.

Description of drawings

Fig. 1 is the decomposition diagram that is used for the filter container of collecting cell according to an embodiment of the invention;

Fig. 2 shows the skeleton view of the connection status of filter container according to an embodiment of the invention;

Fig. 3 is the decomposition diagram of filter unit according to an embodiment of the invention;

Fig. 4 shows the user mode figure of cell filtration operation according to an embodiment of the invention; And

Fig. 5 shows according to the institute isolated cell of embodiments of the invention before being collected on the microslide.

Embodiment

Hereinafter, will describe the present invention in detail with reference to accompanying drawing.

To the filter container that be used for collecting cell according to an embodiment of the invention be described referring to figs. 1 to Fig. 5.The filter container that is used for collecting cell comprises upper container body 100 and following vessel 200, and following vessel 200 is being connected to upper container body 100 by attached/removably from the below.

Say that at length upper container body 100 comprises: accomodating unit 110, it has opens so that hold the top of the solution that contains cell C; Insert unit 120, it is arranged under the accomodating unit 110 and opens downwards; And diaphragm element 150, its through be provided with accomodating unit 110 with insert separation between the unit 120 and have the mesh structure so that the foreign matter that leaches in the solution to be contained.

That is, in first half body 100, accomodating unit 110 and insert unit 120 and be separately positioned in top and the lower part with respect to the diaphragm element 10 in the middle of being horizontally set on, and accomodating unit 110 opens up, and insert unit 120 and open down.

Following vessel 200 is formed by filter unit 210 and exhaust unit 220; The top of filter unit 210 connects to be contained in inserts in the unit 120, and the cell C through diaphragm element 150 is received on the upper surface of filter unit 210; Exhaust unit 220 is arranged under the filter unit 210 with the solution of discharging through filter unit 210.

Filter unit 210 is formed by the following: admit groove 212, it is formed on down in the upper surface of vessel 200 and comprises a plurality of discharge orifice 212a; Filter member 215, it has the mesh structure and is arranged on admits on the groove 212 and separates solution and cell C through diaphragm element 150 with collecting cell C; And supporting member 216, it is arranged on filter member 215 and admits between the groove 212 and the elasticity supporting filter member 215 that makes progress.

Supporting member 216 can be formed by the compressed sponge material, so that except supporting filter member 215, also discharge cell-preservation liquid, but supporting member 216 is not limited thereto.

Diaphragm element 150 and filter member 215 have the mesh structure that contains a plurality of holes.Preferably, the mesh that had of filter member 215 can be more than diaphragm element 150.

Diaphragm element 150 lets the cell C that is contained in the cell-preservation liquid pass through; And stop granularity greater than the foreign matter of the cell C that is contained in the cell-preservation liquid to leach foreign matter; And filter member 215 is used for helping to be received in through the cell C of diaphragm element 150 the top of filter member 215, and only solution gives off through the discharge orifice 212a under the filter member 215.

For example, the mesh number of diaphragm element 150 can be 150 to 300 meshes, and the mesh number of filter member 215 can be 500 meshes or more than 500 meshes.

In addition, upper container body 100 and down each comprises backstop in the vessel 200, upper container body 100 takes place to slide and rotates when preventing that upper container body 100 and following vessel 200 from interconnecting.

Backstop comprises: projection 230, and it is from the external peripheral surface projection of following vessel 200; And groove part 130, it is formed in the inner circumferential surface of insertion unit 120 of upper container body 100, makes projection 230 be inserted in the groove part 130.

In addition, a plurality of

projections

140 and 240 of space are respectively formed on the outside surface of upper container body 100 and following vessel 200.

Projection

140 and 240 formation make upper container body 100 and vessel 200 can be easily by fixing when the clamping apparatus (not shown) that uses testing fixture carry out the clamping operation down, said testing fixture clamping upper container body 100 and vessel 200 and collecting cell C down.

That is,

projection

140 and 240 be used for clamping apparatus clamping upper container body 100 and down the external peripheral surface of vessel 200 prevent upper container body 100 and waving of vessel 200 down to promote upper container body 100 and down during vessel 200.

Hereinafter with description have said structure according to the operation that is used for the filter container of collecting cell of the present invention.

Be used for the filter container of collecting cell; Be inserted into through the top that will descend vessel 200 in the insertion unit 120 of upper container body 100 so that the filter unit 210 of vessel 200 is arranged under the diaphragm cell 150 of upper container body 100 down, assemble upper container body 100 and vessel 200 down.

The projection 230 of following vessel 200 is inserted in the inner circumferential surface of upper container body 100 in the formed groove part 130, so that prevent upper container body 100 rotation of sliding takes place.

After above-mentioned assembly operation; When the cell-preservation liquid of the solution container at accomodating unit 110 places that upper surface is opened is poured in the filter container; The cell C that is contained in cell-preservation liquid and the cell-preservation liquid is through diaphragm element 150 and flow to and insert in the unit 120, but the foreign matter that is contained in the cell-preservation liquid is through diaphragm element 150 and do not leached.

Then; Flow to the cell-preservation liquid that inserts in the unit 120 and passed through filter member 215 and supporting member 216; Flowing in the exhaust unit 220, and flow to the cell C that inserts in the unit 120 and leached with in the top that is received in filter member 215 through discharge orifice 212a.

Then, the cell-preservation liquid that has flow in the exhaust unit 220 gives off through the hose member that is connected to exhaust unit 220 in addition.

To move slidably by the microslide S that glass forms to be sent to the part on the filter member 215; And then; Promote down vessel 200 through use clamping apparatus, and the cell C that is adhered on the top with filter member 215 is attached on the lower surface with the microslide S that collects.

Here, supporting member 216 is elasticity supporting filter member 215 upwards, whereby, and protection microslide S when filter member 215 and microslide S are in contact with one another, and improve the adhesiveness of cell C.

Before transmitting microslide S through other clamping instrumental elevation upper container body 100 so that separate with following vessel 200.

After this, can check that collected cell C has no abnormal through using microscope to observe the cell C that is adhered on the lower surface of microslide S.

Therefore; According to the present invention; The filter container that is used for collecting cell has can be attached/structure that removably connects; Wherein can separate and assemble with the lower part on the top of filter container, and the cell that is contained in the cell-preservation liquid and foreign matter can separate through diaphragm element 150 and filter member 215 easily, and institute's isolated cell can be collected on the microslide S easily.

Claims

1. filter container that is used for collecting cell comprises:

The upper container body; It comprises have the accomodating unit of opening so that holding the top of the solution that contains cell, the insertion unit that is arranged under the said accomodating unit and opens downwards, and through being provided with to divide the diaphragm element of said accomodating unit and said insertion unit; And

Following vessel; It comprises having and removably connects being contained in the filter unit of top and the upper surface of admitting the cell through said diaphragm element in the said insertion unit, and is arranged under the said filter unit and has the exhaust unit in the space that is used to discharge the said solution through said filter unit.

2. the filter container that is used for collecting cell according to claim 1 is characterized in that said filter unit comprises:

Admit groove, it is formed in the upper surface of said vessel down, and wherein a plurality of discharge orifices are formed in the said admittance groove;

Filter member, it is arranged on the said admittance groove and separates said solution and said cell through said diaphragm element to collect said cell; And

Supporting member, it is arranged between said filter member and the said admittance groove with the said filter member of resiliency supported that makes progress.

3. the filter container that is used for collecting cell according to claim 1; It is characterized in that each also comprises backstop in said upper container body and the said down vessel, with prevent said upper container body with said down when vessel interconnects said upper container body take place to slide and rotate.

4. the filter container that is used for collecting cell according to claim 3 is characterized in that said backstop comprises:

Projection, it is from the said external peripheral surface projection of vessel down; And

Groove part, it is formed in the inner circumferential surface of said upper container body, makes said projection be inserted in the said groove part.

5. the filter container that is used for collecting cell according to claim 2 it is characterized in that said filter member and said diaphragm element have the mesh structure, and the mesh that said filter member had is more than said diaphragm element.

6. the filter container that is used for collecting cell according to claim 1; It is characterized in that each also comprises a plurality of projections in said upper container body and the said vessel down, said projection is along said upper container body and the said corresponding external peripheral surface space of vessel down.