CN102775218A - Method for preparing plant nutrient solution from sludge microproteins - Google Patents
Method for preparing plant nutrient solution from sludge microproteins Download PDFInfo
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- CN102775218A CN102775218A CN2012102895074A CN201210289507A CN102775218A CN 102775218 A CN102775218 A CN 102775218A CN 2012102895074 A CN2012102895074 A CN 2012102895074A CN 201210289507 A CN201210289507 A CN 201210289507A CN 102775218 A CN102775218 A CN 102775218A
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Abstract
The invention relates to a method for preparing a plant nutrient solution from sludge microproteins, which comprises the following steps: 1) hydrolyzing activated sludge under alkaline conditions, carrying out solid-liquid separation, and collecting the filtrate; 2) regulating the pH value of the filtrate obtained in the step 1) to 4-7 with a pH regulator, concentrating, and removing impurities to obtain a microprotein solution; and 3) adding manganese sulfate, zinc sulfate, magnesium sulfate and ferrous sulfate into the microprotein solution obtained in the step 2), mixing, sufficiently stirring, keeping the temperature of 40-60 DEG C for 20-40 minutes, adding boric acid, and sufficiently stirring to obtain the plant nutrient solution. The invention solves the problem of pollutant emission in sludge treatment, and can recycle resources; and the prepared plant nutrient solution is beneficial to development of trees and flowers, and has an obvious action on improving the soil environment for plant growth.
Description
Technical field
The present invention relates to the preparation method of a plant nutrient liquor, especially a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid.
Background technology
At present, the main mode of sludge treatment is burning, mummification, landfill or hydrolysis etc., and wherein the heat energy of burning or mummification mode labor produces comparatively serious atmospheric pollution simultaneously; The mode of landfill or hydrolysis can produce a large amount of percolates, is the important environmental pollutant of sludge treatment industry, and the sludge treatment problem has become the urban environment problem that we can't avoid.
The output of the annual microorganism active mud of China has 3,000 ten thousand tons approximately; General its protein and amino acid composition account for more than 70% of mud dry-matter; Be an important biological organic matter resource, how properly dispose sewage plant sludge, and it is effectively utilized as a kind of new resource; Turn waste into wealth, become the focus of relevant departments' research;
There is the pertinent literature introduction mud to be carried out directly compost fermentation is after-applied to increase soil fertility in the soil at present; Though this mode can short-term increase soil fertility; But can cause soil knot plate because of the introducing of a large amount of inoculating microbes and mineral substance for a long time; Serious problems such as sclerosis are not approved by numerous its people now.
The objective of the invention is to overcome the deficiency of prior art,, further be modulated into the plant nutrition liquid of cultivating forest and flowers reclaiming the microbial proteinous that extracts in the mud thermokalite hydrolysis treatment process.The filtrating that the hydrolysis of mud thermokalite produces; Can further be hydrolyzed into protein, polypeptide or the amino acid solution of different molecular weight grade, not only can not injure the germination of plant seed, also can play significant improvement effect the edatope of plant-growth; Therefore the present invention has not only solved the problem of sludge treatment pollutant emission; Can make resource regeneration again, be modulated into plant nutrition liquid, help the growth of forest and flower plant; And the edatope to plant-growth plays significant improvement effect, is a kind of significant achievement that breaks through traditional land reclamation and vegetable fertilizer supply.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid is provided.
The technical scheme that the present invention adopts is:
A kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid comprises the steps:
1), with active sludge hydrolysis under alkaline condition, carry out solid-liquid separation behind the hydrolysis reaction, collect filtrating (this filtrating is sludge microbe intracellular fluid and moisture);
2), with pH value regulator regulating step 1) pH value of filtrate is 6-7, concentrates, and concentrates the back and removes impurity, promptly gets microbial proteinous solution;
3), to step 2) add manganous sulfate, zinc sulfate, sal epsom, ferrous sulfate in the microbial proteinous solution that makes, after the mixing, fully stir, kept 20-40 minute down at 40-60 ℃, and then add boric acid, fully stir and obtain this plant nutrition liquid.
Preferably, said active sludge is selected from sewage work's organic active mud, the organic bed mud in rivers and lakes, and the eutrophic lake blue-green algae assembles one or more in the homogenate.
Preferably, said pH value regulator is a nitric acid.Volatility problem and cl ions that the selection of nitric acid has been avoided using HCl to bring are introduced the later stage to the infringement problem that soil brings, and have avoided use H simultaneously
2SO
4The problem that causes the single fertile solubleness of other solubilities to reduce.
Preferably, described enrichment step is meant and in rotatory evaporator, carries out concentrating under reduced pressure that concentration time is 1-3 hour (concentration according to required microbial proteinous solution is adjusted).
Preferably, the step of said removal impurity is a high speed centrifugation, under the 7500rpm rotating speed centrifugal 15 minutes, get supernatant, and be microbial proteinous solution.
Preferably, the addition of manganous sulfate, zinc sulfate, sal epsom, ferrous sulfate, boric acid is a benchmark with the content of the microbial proteinous in the microbial proteinous solution, according to mass fraction; When microbial proteinous is 100 parts; Manganous sulfate is 7-10 part, and zinc sulfate is 2-3 part, and sal epsom is 40 parts; Ferrous sulfate is 1 part, and boric acid is 40-50 part.In 1000 parts of: the microbial proteinous solution of 10% mass concentration, need to add manganous sulfate 7-10 part, zinc sulfate 2-3 part, 40 parts in sal epsom, 1 part in ferrous sulfate, boric acid 40-50 part.
Preferably, said step 1) is reaction that active sludge is hydrolyzed under the condition of calcareous catalyzer, temperature 115-130 ℃, hydrolysis 30min-8h, and the press filtration of the laggard andante frame of hydrolysis reaction is collected and is filtrated.More preferably, said filter press generally uses the pressure of 0.4-0.8MPa, keep-ups pressure 30-120 minute.
Consider preferred steps 1 simultaneously from factor such as cost) filtrating is that crude protein concentration is at the raw material more than 1.5%;
The present invention also provides a plant nutrient liquor, and this plant nutrition liquid is prepared by above-mentioned any method.
The present invention also provides the application of above-mentioned plant nutrition liquid in plant growing, and said plant nutrition liquid is used for cultivation of plants after diluting through 50-500 times of water.
The beneficial effect that the present invention had:
The present invention has not only solved the problem of sludge treatment pollutant emission; Can make resource regeneration again; The plant nutrition liquid that makes helps the growth of forest and flower plant; And the edatope to plant-growth plays significant improvement effect, is a kind of significant achievement that breaks through traditional land reclamation and vegetable fertilizer supply.
The present invention under the effect of calcareous medicament and HTHP, impels the sludge microbe cell envelope to break through the approach of thermokalite hydrolysis; The entocyte of higher protein content flows out, and can obtain specific microbial proteinous, and this type material has comparatively significant activation to the microorganism active in the soil; Promote the breeding of soil pathogenic microorganism, and the digestion through mikrobe, like subtilis; Agrobacterium rhizogenes etc.; Make its protein component be decomposed into amino acid,, play the effect that promotes plant-growth by plant absorbing.Therefore; Utilize proteic this specific character of sludge microbe; And the trace element and the nitrogen phosphorus potassium component of adding other, the plant nutrition liquid product of soil improvement and plant nutrition double effects has been gathered in the development cost invention, and has solved the problem of environmental pollution in the sludge treatment simultaneously.
Embodiment
Below in conjunction with specific embodiment the present invention is described further, but does not limit protection scope of the present invention.
Embodiment 1
The sludge treatment facility of certain urban wastewater treatment firm adopts the mode of thermokalite hydrolysis to carry out the processing of mud and the extraction of resource product, uses the unslaked lime catalyzer, and the addition of unslaked lime is 20% of a mud butt quality; Temperature at 128 ℃; 0.25MPa pressure condition under, hydrolysis 40 minutes, and use 0.6Mpa pressure to carry out filter press and collect filtrating after 60 minutes; Crude protein content is 1.87 in the filtrating, and pH is 12.
In above-mentioned filtrating, add rare nitric acid, measure pH while stirring, when pH is 7, stop to add rare nitric acid, continue to stir 30 minutes; After thoroughly even, be delivered to rotatory evaporator by devices such as impeller pumps, the adjustment temperature concentrates at 91 ℃; Till obtaining crude protein concentration and being 10% albumen homogenate, leave standstill be cooled to room temperature after, albumen homogenate is delivered to supercentrifuge; Under the rotating speed of 7500rpm centrifugal 15 minutes, get supernatant, be microbial proteinous solution.Get 1000 parts of microbial proteinous solution, add 8 parts of manganous sulfates, 3 parts in zinc sulfate, 40 parts in sal epsom, 1 part in ferrous sulfate mixes, and keeps 30 minutes down at 40-60 ℃, adds 48 parts of boric acid afterwards again, promptly can be made into plant nutrition liquid.
The finished product plant nutrition liquid is tawny or sorrel liquid; Microbial proteinous content is 9%, and organic content is about 35%, contains the required various elements of plant-growth; And heavy metal content is Cd≤0.01%; As≤0.002%, Pb≤0.002% meets the national Ministry of Agriculture " green food fertilizer usage criteria " requirement.
Embodiment 2 (application implementation example)
Use made plant nutrition liquid among the embodiment 1, carry out the planting experiment of cucumber in plot experiment Tanaka, test is divided into two groups (experimental group 1 and experimental group 2), and experimental group 1 is 10m with experimental group 2 cultivated areas
2To use the crude protein concentration of embodiment 1 be that 10% albumen homogenate is appended as base manure for experimental group 1 before the sowing, and experimental group 2 uses the cow dung fertilizers to apply as base manure, and the base manure addition of experimental group 1 and experimental group 2 is that 35cm appends at the place 5kg base manure under topsoil; Sowing time is the first tenday period of a month in May; Apply liquid fertilizer respectively at early June, early July behind the germinating to seedling, experimental group 1 is with the plant nutrition liquid of embodiment 1 preparation, and use with the mode that sprays irrigation the dilution back; Experimental group 2 is got the mode that supernatant homogenate liquid sprays irrigation and is used leaving standstill after the cow dung fertilizer dilution; Experimental group 1 is every month with experimental group 2 and sprays once, and each usage quantity is 1kg, and thin up is sprayed to 50L.Gather in the crops August.During results, experimental group 1, mean blade width 32cm, solid average substance is 412g, maximum substance reaches the 592g/ root, first batch of 10m
2Output is 410 jin; Experimental group 2, mean blade width 24cm, solid average substance is 279g, maximum substance is 380g, first batch of 10m
2Output is 280 jin.
The cucumber that present method plantation obtains is not because of using agricultural chemicals, so the non agricultural chemical residuum influence in the planting process; Detect its nitrate content less than 300mg/L; Heavy metal content Cd≤1.0mg/L, Pb≤3.0mg/L.Each item index meets the standard of pollution-free vegetable, and heavy metal content meets European Union's food heavy metal standard limited value.
The above; It only is preferred embodiment of the present invention; Be not that technical scheme of the present invention is done any pro forma restriction; Every foundation technical spirit of the present invention all still belongs in the scope of technical scheme of the present invention any simple modification, equivalent variations and modification that above embodiment did.
Claims (9)
1. a method of utilizing sludge microbe protein Preparation plant nutrition liquid is characterized in that: comprise the steps:
1), with active sludge hydrolysis under alkaline condition, carry out solid-liquid separation behind the hydrolysis reaction, collect filtrating;
2), with pH value regulator regulating step 1) pH value of filtrate is 6-7, concentrates, and concentrates the back and removes impurity, promptly gets microbial proteinous solution;
3), to step 2) add manganous sulfate, zinc sulfate, sal epsom, ferrous sulfate in the microbial proteinous solution that makes, after the mixing, fully stir, kept 20-40 minute down at 40-60 ℃, and then add boric acid, fully stir and obtain this plant nutrition liquid.
2. according to the said a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid of claim 1; It is characterized in that: said active sludge is selected from sewage work's organic active mud; The organic bed mud in rivers and lakes, the eutrophic lake blue-green algae assembles one or more in the homogenate.
3. according to claim 1 or 2 said a kind of methods of utilizing sludge microbe protein Preparation plant nutrition liquid, it is characterized in that: said pH value regulator is a nitric acid.
4. according to each said a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid of claim 1-3, it is characterized in that: described enrichment step is meant and in rotatory evaporator, carries out concentrating under reduced pressure that concentration time is 1-3 hour.
5. according to each said a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid of claim 1-4; It is characterized in that: the step of said removal impurity is a high speed centrifugation; Under the 7500rpm rotating speed centrifugal 15 minutes, get supernatant, be microbial proteinous solution.
6. according to each said a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid of claim 1-5, it is characterized in that: the addition of said manganous sulfate, zinc sulfate, sal epsom, ferrous sulfate, boric acid is a benchmark with the content of the microbial proteinous in the microbial proteinous solution, according to mass fraction; When microbial proteinous is 100 parts; Manganous sulfate is 7-10 part, and zinc sulfate is 2-3 part, and sal epsom is 40 parts; Ferrous sulfate is 1 part, and boric acid is 40-50 part.
7. according to each said a kind of method of utilizing sludge microbe protein Preparation plant nutrition liquid of claim 1-6; It is characterized in that: said step 1) is reaction that active sludge is hydrolyzed under the condition of calcareous catalyzer, temperature 115-130 ℃; Hydrolysis 30min-8h; Filtrating is collected in the press filtration of the laggard andante frame of hydrolysis reaction.
8. a plant nutrient liquor, it is characterized in that: this plant nutrition liquid is prepared by any method of claim 1-7.
9. the application of the said plant nutrition liquid of claim 8 in plant growing is characterized in that: said plant nutrition liquid is used for cultivation of plants after diluting through 50-500 times of water.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103564178A (en) * | 2013-10-11 | 2014-02-12 | 天津市裕川环境科技有限公司 | Preparation equipment, preparation method and application of thallus compound amino acid feed additive |
| CN109134003A (en) * | 2018-09-21 | 2019-01-04 | 桂林润泰生物科技有限公司 | A kind of processing technology using municipal sludge production cream fertilizer base fluid |
| CN111943758A (en) * | 2020-08-25 | 2020-11-17 | 北京汇园生态科技有限公司 | Plant microbial exciter |
| CN111960889A (en) * | 2020-08-25 | 2020-11-20 | 北京汇园生态科技有限公司 | Extracting solution for preventing and treating plant diseases and insect pests |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103564178A (en) * | 2013-10-11 | 2014-02-12 | 天津市裕川环境科技有限公司 | Preparation equipment, preparation method and application of thallus compound amino acid feed additive |
| CN109134003A (en) * | 2018-09-21 | 2019-01-04 | 桂林润泰生物科技有限公司 | A kind of processing technology using municipal sludge production cream fertilizer base fluid |
| CN111943758A (en) * | 2020-08-25 | 2020-11-17 | 北京汇园生态科技有限公司 | Plant microbial exciter |
| CN111960889A (en) * | 2020-08-25 | 2020-11-20 | 北京汇园生态科技有限公司 | Extracting solution for preventing and treating plant diseases and insect pests |
| CN111960889B (en) * | 2020-08-25 | 2022-04-12 | 北京汇园生态科技有限公司 | Extracting solution for preventing and treating plant diseases and insect pests |
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Application publication date: 20121114 |