CN102755281A - Cosmetic containing microbial modulator - Google Patents
Cosmetic containing microbial modulator Download PDFInfo
- Publication number
- CN102755281A CN102755281A CN2012102421608A CN201210242160A CN102755281A CN 102755281 A CN102755281 A CN 102755281A CN 2012102421608 A CN2012102421608 A CN 2012102421608A CN 201210242160 A CN201210242160 A CN 201210242160A CN 102755281 A CN102755281 A CN 102755281A
- Authority
- CN
- China
- Prior art keywords
- cosmetics
- contain
- skin
- class
- bacillus bifidus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cosmetics (AREA)
Abstract
The invention relates to a cosmetic containing a microbial modulator. The cosmetic comprises 0.5-1wt% of bifidobacterium; the bifidobacterium is adopted as a microecological modulator of the cosmetic, and the produced microecological cosmetic can be used for prompting the metabolism of the skin to achieve the effects of protecting skin and beautifying; and the cosmetic can be industrially produced by virtue of the technique and is beneficial to skin health of people.
Description
Technical field
The present invention relates to contain the cosmetics of microorganism regulator, relate in particular to a kind of cosmetics that contain bacillus bifidus.
Background technology
Microecologic regulator is under the microecology theoretical direction, adjusts little ecological disturbance, keeps microecological balance, improves the skin health level or promotes the probiotic bacteria of skin health state and the goods of metabolite and growth helping matter thereof.Microecology is a new branch of science of setting up over 20 years He grow up, and it is " the life sciences branch of research normal microflora and its host's mutual relation ".Microecology has been illustrated the substantial connection between human existence and development and the microorganism of its coexistence, and it has pointed out people's all one's life all the time with of a great variety, and the microorganism of enormous amount is accompanied together.Data shows that an adult TCS is 1014, and normal flora is that quantity is 1013 in the body, so the people is not an isolated people, and the people is the homobium of himself and microorganism.Discover that the normal strain that the human body skin surface exists can reach over one hundred kind; Topmost staphylococcus epidermidis, propionibacterium, yeast, diphtheroid, excellent bacillus, lactobacillus, bacillus bifidus or the like of comprising; The dynamic equilibrium of these floras is determiners of maintenance human body skin health and eubolism, and this point is the basic conception of skin microecology.Inherent micro-ecological bacterial crowd and skin are under a kind of dynamic equilibrium, can think the skin of health.Make skin keep microecological balance just to be able to bacterium and mend bacterium; Control bacterium with bacterium because skin since self, environment, medicine, their microecological balance is lacked of proper care through regular meeting; Must make it to restore balance from extraneous probiotic supplemented or probiotics factor at any time, improve health.
Chinese patent 97112152.4 discloses a kind of cosmetics that contain the microorganism regulator, it is characterized in that it is 1% ~ 10% youth's Bick candida mycoderma and/or Rhodotorula rubra HMC or its metabolite that said cosmetics contain percentage by weight.But saccharomycetic shortcoming is: yeast is the transition bacterium, it can not field planting in skin, so can not participate in the Human Physiology circulation, have only continual supply to play effect to skin, be further improved.
Summary of the invention
The present invention is directed to above-mentioned deficiency, a kind of physiology circulation that can participate in human body is provided, can fundamentally play skin-protecting face nursing, the cosmetics that contain the microorganism regulator of the effect of adjustment skin microecological balance.
The present invention is for solving the problems of the technologies described above; Following technical scheme is provided: a kind of cosmetics that contain the microorganism regulator; It is characterized in that in described cosmetics, containing percentage by weight and be 0.5% ~ 1% bacillus bifidus, described bacillus bifidus is a hebephrenictype bacillus bifidus DM8504 bacterial strain.
As of the present invention preferred, described bacillus bifidus is dead bacterium or viable bacteria.
As of the present invention preferred, described cosmetics are cleaning type, nursing type, beauty treatment/modification class.
As of the present invention preferred, the cleaning class is cleansing milk, facial film, cleansing cream.
As of the present invention preferred, the nursing class is cold cream frost, emulsion, astringent.
As of the present invention preferred, beauty treatment/modification class is muffin, kermes, eye shadow, perfume.
The production technology of microecologic regulator bacillus bifidus of the present invention is known, and production technology may further comprise the steps:
Slant medium: adopt the BS slant medium, PH7.2-7.4, the inclined-plane is put in sterilization in 121 ℃, 30 minutes after the packing, and empty the cultivation after 2 days preserved in refrigerator.
Liquid seed culture medium: adopt the BS fluid medium.PH7.2-7.4 gets 500ml and is sub-packed in the infusion bottle, and sterilization in 121 ℃, 30 minutes is for use.
Get DM8504 lyophilizing pipe, with the lyophilizing pipe sterilize, sever, add activator, picking bacterium liquid is streak culture, isolates colonies typical and passes slant culture, behind assay approval, use as female inclined-plane.
Be inoculated in sub-inclined-plane by female inclined-plane, sub-inclined-plane under anaerobic 37 ℃ cultivated 48 hours.
Being inoculated in behind the seed bottle 37 ℃ by sub-inclined-plane cultivated 48 hours.Inspection should meet the standard type of hebephrenictype bacillus bifidus under the sub-flat inclined mirror after the cultivation: growth promoter is good; Seed culture fluid should be sepia after cultivating, and a small amount of canescence solids precipitation is arranged at the bottom, and seed liquor PH should be more than 4.5; Mirror is observed down and should be G-, is shaft-like uneven thalline.
Seed liquor is concentrated in the bottle,suction, be inoculated in the fermentation tank with the pressure reduction vaccination ways.
Before last jar total air filter, branch air filter, fermentation tank and each pipeline are carried out disinfection, the time is 45 minutes, and pressure is 0.2MPa.
Begin fermentation culture after the inoculation, condition of culture is 37 ± 1 ℃ of temperature, and pressure 0.02-0.04MPa stirred time 22-26 hour 5 minutes/3 hours.Record fermentation temperature, pressure, mixing time and situation such as number of times, PH, incubation time and fermentation liquid metabolism pollution.
Put a jar time with parameter determining such as fermentation liquid pollution condition, time, PH, microscopies.Generally reduce to 4.5 and can put jar when following cultivating after 22 hours PH.
Put a jar culture fluid be compressed air be pressed into flexible centrifuge carry out centrifugal, with valve adjustment centrifugal liquid flow, generally with in the centrifugal liquid not mycetome be advisable.Concentrator bowl is taken out in centrifugal back, sends into the dry thalline that takes out.
Scrape the bacterium mud of getting in the concentrator bowl, weighing.Get 5% sodium glutamate and 1 part of 5% defatted milk powder that 1 part in bacterium mud adds 1 to 1.5 part, fully mix, put not in the stainless steel disc.Material to be dried is put into freezer dryer carry out drying.
After lyophilizing finished, freeze drying box still was in vacuum state, must feed aseptic dry air or nitrogen before the product outlet and can open valve taking-up product, preserved with lower seal for 4 ℃.The product water content should be controlled at below 3%, promptly produces hebephrenictype bacillus bifidus DM8504.
Beneficial effect of the present invention: the present invention adopts the microecologic regulator of bacillus bifidus as cosmetics; Little skin-care ecological article of producing can promote the metabolism of skin; Reach the effect of skin-protecting face nursing, and this technology can organize the cosmetics suitability for industrialized production, benefit the health of human body skin.
The specific embodiment
A kind of cosmetics that contain the microorganism regulator; It is characterized in that in described cosmetics, containing percentage by weight and be 0.5% ~ 1% bacillus bifidus, described bacillus bifidus is a hebephrenictype bacillus bifidus DM8504 bacterial strain, and described bacillus bifidus is dead bacterium or viable bacteria; Described cosmetics are cleaning class, nursing class, beauty treatment/modification class; The cleaning class is cleansing milk, facial film, cleansing cream, and the nursing class is cold cream frost, emulsion, astringent, and beauty treatment/modification class is muffin, kermes, eye shadow, perfume.
Embodiment 1: contain the cleansing milk of microecologic regulator, (prescription) wherein each composition weight is percentage ratio: HANSHENGJIAO 0.4%, and AES 7%, Semen Ginkgo glycoside 1%; Microecologic regulator 1%, 1-3 butanediol 2%, 200 7%; Stearic acid 4%, pearly-lustre thickening agent 620 1.6%, C-30 5.12%; Essence 0.2%, antiseptic 901 1.5%, deionized water 72% (3% evaporation capacity is arranged).
Production technology: weighing deionized water 72% adds in the retort, is heated to 80 ℃-85 ℃, adds Semen Ginkgo glycoside (production of Jilin pharmaceutical factory) and microecologic regulator of the present invention; Add 1-3 butanediol (production of Shanghai Henkel Corp.); After treating off-bottom, add thickening agent HANSHENGJIAO (production of Shanghai Henkel Corp.), treat to add after the off-bottom AES (production of Shanghai Henkel) and add 200 and stearic acid simultaneously; Whole process stirs and continues, and keeps temperature more than 70 ℃; C-30 is slowly joined retort after with water dissolution, and temperature maintenance stirs at 70 ℃ and continues 10 minutes, opens cooling water and slowly lowers the temperature; Treat that temperature reduces to 50 ℃ and add pearly-lustre thickening agents 620; Add antiseptic 901 simultaneously, essence treats to process when temperature is reduced to 40 ℃ cleansing milk of the present invention.
The milk of washing one's face: observe 1500 many cases altogether, 460 examples are wherein filled in the observation form.
Experimental period is 25 days, criterion of therapeutical effect: skin reaches salubrious, flexible, preserve moisture to effectively.
Embodiment 2: contain the cream kind of microecologic regulator, wherein each composition weight is percentage ratio, oil phase: stearic acid 5%, hexadeca-octadecyl alcohol 8%; Oleum Hippophae 3%, Adeps Bovis seu Bubali resin 4%, lanoline 8%, dimethicone 5%; Glycerol 3%, 68 1%, OP2%, white oil 1%; Water: Semen Ginkgo glycoside 1%, the ginsenoside 1%; Microecologic regulator 1%; Other: essence 0.2%, antiseptic 901 3%, deionized water 56.8% (3% is used for evaporation).
Production technology: with the accurate weighing of oil phase raw material, drop in the oil phase jar, heat while stirring to 85 ℃-90 ℃ constant temperature 5-10 minutes; With the accurate weighing of water raw material, drop in the water jar, start and be heated with stirring to 90 ℃-95 ℃, constant temperature 5-10 minutes; Be preheated to 45 ℃-50 ℃ to the vacuum emulsification jar, be evacuated to 0.05-0.06Mpa; The ready oil phase raw material of elder generation's suction, the ready water raw material of suction is opened quick homogenizer and blender simultaneously again; Homogenizing 8 minutes, stir speed (S.S.) are 80rpm, stir after 10 minutes; Open cooling water and slowly be cooled to 75 ℃, opened the homogenizer homogenizing again 5 minutes, stir and carry out always.When temperature is reduced to 60 ℃, add antiseptic, when reducing to 50 ℃, add essence, when temperature is reduced to 40 ℃, stop to stir, process the cream kind cosmetics that the present invention obtains.
Cream kind:
With the skin whitener is example:
Observe 1000 many cases altogether, 360 examples are wherein filled in the observation form.
Be equally divided into two groups, smear the skin whitener that contains microecologic regulator (bacillus bifidus) for one group; Another group is smeared traditional skin whitener.The time of smearing is three months.
Criterion of therapeutical effect: recovery from illness: skin lesion all disappears, and it is normal that color of the leather recovers.
Produce effects: the skin lesion area dwindles more than 70%, and color of the leather obviously disappears.
Effectively: the skin lesion area dwindles more than 50%, the color of the leather minor regression.
Invalid: the skin lesion area dwindles below 30%, color of the leather minor regression or do not have improvement.
Therapeutic effect contrast after three months, like following table:
Embodiment 3: the mass percent that contains the foundation cream apoplexy due to endogenous wind essence of microecologic regulator is oil phase: hexadeca-octadecyl alcohol 8%, and triglyceride 5%, stearic acid 8%, dimethicone 3%, white oil 1.5%, lanoline 4%, OT 2%; Water: microecologic regulator 0.5%, powder base 1%; Other: antiseptic 3%, essence 0.2%, deionized water 66.8% (3% is used for evaporation).
Production technology: with the accurate weighing of oil phase raw material, drop in the oil phase jar, heat while stirring to 80 ℃-85 ℃ constant temperature 10 minutes; With the accurate weighing of water raw material, drop in the water jar, be heated to 85 ℃-90 ℃, constant temperature 10 minutes; Be preheated to 45 ℃ to the vacuum emulsification jar, be evacuated to-0.06Mpa; The ready oil phase raw material of elder generation's suction is opened and is stirred 60rpm, homogenizing 2000rpm, the ready water raw material of suction simultaneously, and stir speed (S.S.) is 80rpm, homogenizing 3000rpm, homogenizing stirred and carry out always after 10 minutes; After homogenizing stops, open cooling water and begin to cool down, mixing speed is slowly reduced to 60rpm, continue to be cooled to 50 ℃ after, add essence, antiseptic, stir and reduce to 40rpm, when temperature is cooled to 40 ℃, stop to stir, process the foundation cream class cosmetics that the present invention obtains.
Foundation cream class: with the sun-proof example that is isolated into: observe 500 many cases, 180 examples are wherein filled in the observation form.
Be equally divided into two groups, one group is used the sun-proof isolation that contains microecologic regulator (bacillus bifidus); Another group is used traditional sun-proof isolation.Experimental period is August.
Criterion of therapeutical effect: skin does not have influence for effective:
Claims (7)
1. cosmetics that contain the microorganism regulator is characterized in that in described cosmetics, containing percentage by weight and are 0.5% ~ 1% bacillus bifidus.
2. a kind of cosmetics that contain the microorganism regulator according to claim 1 is characterized in that described bacillus bifidus is a hebephrenictype bacillus bifidus DM8504 bacterial strain.
3. a kind of cosmetics that contain the microorganism regulator according to claim 2 is characterized in that described bacillus bifidus is dead bacterium or viable bacteria.
4. a kind of cosmetics that contain the microorganism regulator according to claim 1 is characterized in that described cosmetics are cleaning class, nursing class, beauty treatment/modification class.
5. a kind of cosmetics that contain the microorganism regulator according to claim 4 is characterized in that the cleaning class is cleansing milk, facial film, cleansing cream.
6. a kind of cosmetics that contain the microorganism regulator according to claim 4 is characterized in that the nursing class is cold cream frost, emulsion, astringent.
7. a kind of cosmetics that contain the microorganism regulator according to claim 4 is characterized in that beauty treatment/modification class is muffin, kermes, eye shadow, perfume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102421608A CN102755281A (en) | 2012-07-13 | 2012-07-13 | Cosmetic containing microbial modulator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102421608A CN102755281A (en) | 2012-07-13 | 2012-07-13 | Cosmetic containing microbial modulator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102755281A true CN102755281A (en) | 2012-10-31 |
Family
ID=47050101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102421608A Pending CN102755281A (en) | 2012-07-13 | 2012-07-13 | Cosmetic containing microbial modulator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102755281A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251547A (en) * | 2013-05-02 | 2013-08-21 | 华南理工大学 | Activated probiotic skin cream and preparation method thereof |
| EP3052077A4 (en) * | 2013-10-03 | 2017-04-26 | ELC Management LLC | Topical preparations comprising grape seed, folic acid, biotin, bifidobacterium longum and echinacea purpurea |
| CN110478309A (en) * | 2019-09-27 | 2019-11-22 | 无锡宾西利悦科技有限公司 | A kind of composition of the shining face of tender skin |
| CN110876708A (en) * | 2019-11-25 | 2020-03-13 | 无锡宾西利悦科技有限公司 | Composition for repairing damaged cells |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101090706A (en) * | 2004-10-04 | 2007-12-19 | 奥里尔股份有限公司 | Cosmetic or dermatologic composition against dry and/or sensitive skin |
| CN101232867A (en) * | 2005-08-01 | 2008-07-30 | 欧莱雅 | Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin |
| CN101254164A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Cosmetic or dermatological composition comprising a cell culture medium |
| CN101380289A (en) * | 2007-09-04 | 2009-03-11 | 欧莱雅 | Cosmetic use of a Bifidobacterium species lysate for treating dry skin |
| CN101904872A (en) * | 2009-03-04 | 2010-12-08 | 奥里尔股份有限公司 | The application of probiotic microorganisms for limiting cutaneous irrittations |
| CN102105132A (en) * | 2008-07-29 | 2011-06-22 | 欧莱雅 | Cosmetic use of microorganisms for the treatment of oily skin |
| CN102131495A (en) * | 2008-07-29 | 2011-07-20 | 欧莱雅 | Cosmetic use of microorganism(s) for treatment of scalp disorders |
| CN102477410A (en) * | 2010-11-30 | 2012-05-30 | 株式会社爱茉莉太平洋 | Composition containing bifidobacterium extract for stem cell performance increasing of fat source stem cell and for skin cell proliferation |
-
2012
- 2012-07-13 CN CN2012102421608A patent/CN102755281A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101090706A (en) * | 2004-10-04 | 2007-12-19 | 奥里尔股份有限公司 | Cosmetic or dermatologic composition against dry and/or sensitive skin |
| CN101232867A (en) * | 2005-08-01 | 2008-07-30 | 欧莱雅 | Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin |
| CN101254164A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Cosmetic or dermatological composition comprising a cell culture medium |
| CN101380289A (en) * | 2007-09-04 | 2009-03-11 | 欧莱雅 | Cosmetic use of a Bifidobacterium species lysate for treating dry skin |
| CN102105132A (en) * | 2008-07-29 | 2011-06-22 | 欧莱雅 | Cosmetic use of microorganisms for the treatment of oily skin |
| CN102131495A (en) * | 2008-07-29 | 2011-07-20 | 欧莱雅 | Cosmetic use of microorganism(s) for treatment of scalp disorders |
| CN101904872A (en) * | 2009-03-04 | 2010-12-08 | 奥里尔股份有限公司 | The application of probiotic microorganisms for limiting cutaneous irrittations |
| CN102477410A (en) * | 2010-11-30 | 2012-05-30 | 株式会社爱茉莉太平洋 | Composition containing bifidobacterium extract for stem cell performance increasing of fat source stem cell and for skin cell proliferation |
Non-Patent Citations (1)
| Title |
|---|
| 刁治民等: "双歧杆菌的生理作用及开发前景", 《青海科技》, no. 06, 31 December 2002 (2002-12-31), pages 37 - 40 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251547A (en) * | 2013-05-02 | 2013-08-21 | 华南理工大学 | Activated probiotic skin cream and preparation method thereof |
| EP3052077A4 (en) * | 2013-10-03 | 2017-04-26 | ELC Management LLC | Topical preparations comprising grape seed, folic acid, biotin, bifidobacterium longum and echinacea purpurea |
| CN110478309A (en) * | 2019-09-27 | 2019-11-22 | 无锡宾西利悦科技有限公司 | A kind of composition of the shining face of tender skin |
| CN110876708A (en) * | 2019-11-25 | 2020-03-13 | 无锡宾西利悦科技有限公司 | Composition for repairing damaged cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108783462A (en) | A kind of industrial process of beneficial bacteria of intestinal tract preparation | |
| CN101623099A (en) | Blueberry probiotic powder and process method thereof | |
| CN112553128B (en) | Probiotic freeze-dried powder and preparation method and application thereof | |
| CN105886439A (en) | Automatic feedback supplementing method for high-density culture of lactic acid bacteria | |
| CN101691568A (en) | Method of producing edible immobilized active probiotics | |
| CN102755281A (en) | Cosmetic containing microbial modulator | |
| CN108902310A (en) | A kind of preparation method of Kefir grains fermentation purple perilla flavor yogurt | |
| CN109588726A (en) | It is a kind of for improving the Lactobacillus casei composition and preparation method thereof of sleep | |
| CN105230782A (en) | Preparation method of hair weed yoghourt | |
| CN108018248B (en) | Lactobacillus casei capable of regulating flora structural disorder caused by antibiotics | |
| RU2261909C1 (en) | BIFIDUS-BACTERIUM CONSORTIUM OF Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium breve USEFUL IN PREPARATION OF FERMENTED-MILK AND NONFERMENTED PRODUCTS, BIOACTIVE SUPPLEMENT, BIFIDOBACTERIA- CONTAINING PRODUCT, COSMETIC AND TOILETRY AGENTS | |
| RU2453591C1 (en) | Strain lactobacillus rhamnosus used for making lactic acid bacillus containing products | |
| CN101671637A (en) | Method for culturing solid nutrient carrier of intestinal probiotics | |
| RU2268931C1 (en) | STRAIN Bifidobacterium bifidum 79-37 USED FOR PREPARING FERMENTED-MILK, FERMENTED AND NONFERMENTED FOODSTUFFS, FERMENTS, HYGIENIC AND COSMETIC AGENTS, BIOLOGICALLY ACTIVE SUPPLEMENTS AND BACTERIAL PREPARATIONS | |
| CN106879738A (en) | Probiotics fermention breast and its manufacture craft | |
| KR100467068B1 (en) | Lactococcus lactis CBT-19, manufacturing method of the culture broth concentration and the cosmetic preparation | |
| RU2453593C1 (en) | Strain lactobacillus helveticus used for making lactic acid bacillus containing products | |
| CN101130759A (en) | Solid culture method of selenium-riched complex probiotics viable bacteria | |
| CN106947726B (en) | High-density fermentation and cold air drying method for lactobacillus casei | |
| RU2261906C1 (en) | BIFIDUS-BACTERIUM STRAIN Bifidobacterium infantis USEFUL IN PREPARATION OF FERMENTED-MILK AND NONFERMENTED PRODUCTS, BIOACTIVE SUPPLEMENT, BIFIDOBACTERIA-CONTAINING PRODUCT, COSMETIC AND TOILETRY AGENTS | |
| KR20150122350A (en) | Solid and liquid phase fermentation method of mushrooms using useful microorganism and functional fermentation composition manufactured by the same | |
| CN108504589A (en) | A kind of compound lactobacillus probiotics and its preparation method and application | |
| RU2460777C1 (en) | Bifidobacterium longum strain used for preparing bifidus products | |
| CN102286396B (en) | Compound microorganism preparation with lactobacillus and preparation method thereof | |
| RU2451729C2 (en) | Bifidobacterium bifidum STRAIN USED TO OBTAIN BIFIDO-CONTAINING PRODUCT |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121031 |