CN102749244A - Tissue transparent reagent, and preparation method and applications thereof - Google Patents
Tissue transparent reagent, and preparation method and applications thereof Download PDFInfo
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- CN102749244A CN102749244A CN2012102672339A CN201210267233A CN102749244A CN 102749244 A CN102749244 A CN 102749244A CN 2012102672339 A CN2012102672339 A CN 2012102672339A CN 201210267233 A CN201210267233 A CN 201210267233A CN 102749244 A CN102749244 A CN 102749244A
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Abstract
The invention discloses tissue transparent reagent, and a preparation method and applications thereof. The tissue transparent reagent comprises the following components in percentage by volume: 0.59 to 0.79 percent of a-pinene, 0.45 to 0.65 percent of b-pinene, 0.45 to 0.65 percent of limonene, 0.45 to 0.65 percent of terpene, 0.45 to 0.65 percent of longifolene, 0.45 to 0.65 percent of caryophyllene, 0.45 to 0.65 percent of aldehyde citral juice, 32.3 to 34.3 percent of d-limonene, 26.7 to 28.7 percent of gamma-terpinene, 0.45 to 0.65 percent of capric aldehyde, 0.45 to 0.65 percent of caprylic aldehyde, 0.45 to 0.65 percent of citral and 32.3 to 34.3 percent of normal butanol. The tissue transparent reagent can replace the conventional transparent reagent xylene, sample tissue treated by the tissue transparent reagent does not become fragile, and is good in the transparent effect.
Description
Technical field
The present invention relates to the tissue samples preparation field, specially refer to a kind of transparency of organization reagent.
Background technology
In medical science and biological study, the transparence of bio-tissue sample helps doctor and scientific research personnel's observation and research.In hyaline tissue's sample preparation field, at present using the widest clarifier is xylene, and xylene is a kind of water white transparency, have the volatile liquid of aromatic odor; Its effect rapidly; Can mix with alcohol, dissolving paraffin can mix with the natural gum of Feng Zangyong again; But must purify moisture when using, otherwise it is muddy that emulsus takes place.For fear of material contracts, should take progressively to carry out the transition to the xylene, i.e. anhydrous alcohol → 1/2 anhydrous alcohol+1/2 xylene → xylene from absolute alcohol.Yet xylene toxicity is bigger, and health is had harm, and it can get into human body through sucking, eat, absorbing through skin.Its toxicity mainly is the spread effect to nervous centralis and neuro vegetative anesthesia and mucous membrane.Suck in a short time can go out to lose face in the higher concentration nuclear weapon and the tangible irritation of the upper respiratory tract, eye conjunctiva and pharyngeal hyperemia, dizziness, feel sick, vomiting, uncomfortable in chest, weakness of limbs, clouding of consciousness, lurch.That weight person can have is restless, twitch or stupor, and what have has an outbreak of hysteria appearance.Long-term contact has neurasthenic syndrome, and the woman worker has menoxenia, and the workman dry skin often takes place, is chapped, dermatitis.The eye and the upper respiratory tract there are spread effect, during high concentration central nervous system are had anesthetic action, serious caused death.Therefore in order to guarantee consumer's safety, European and American countries is all forbidden its application in non-safety area at present.But there is some countries and regions to become global public hazards.
Therefore, prior art awaits to improve and development.
Summary of the invention
In view of the deficiency of above-mentioned prior art, the object of the present invention is to provide a kind of transparency of organization reagent, be intended to solve safety and the environmental issue that present employing tissue samples clarifier xylene exists.
Technical scheme of the present invention is following:
A kind of transparency of organization reagent wherein, according to percent by volume, comprises following component:
A-firpene 0.59-0.79%;
B-firpene 0.45-0.65%;
Limonene 0.45-0.65%;
Terpenes 0.45-0.65%;
Longifolene 0.45-0.65%;
Carypohyllene 0.45-0.65%;
Aldehyde citral juice 0.45-0.65%;
D-taro alkene 32.3-34.3%;
γ-Song Youxi 26.7-28.7%;
Capraldehyde 0.45-0.65%;
Octanal 0.45-0.65%;
Citral 0.45-0.65%;
Normal butyl alcohol 32.3-34.3%.
Described transparency of organization reagent wherein, according to percent by volume, comprises following component:
A-firpene 0.69%;
B-firpene 0.55%;
Limonene 0.55%;
Terpenes 0.55%;
Longifolene 0.55%;
Carypohyllene 0.55%;
Aldehyde citral juice 0.55%;
D-taro alkene 33.3%;
γ-Song Youxi 27.7%;
Capraldehyde 0.55%;
Octanal 0.55%;
Citral 0.55%;
Normal butyl alcohol 33.3%.
A kind of preparation method of aforesaid transparency of organization reagent wherein, may further comprise the steps:
Successively a-firpene, b-firpene, limonene, terpenes, longifolene, carypohyllene, aldehyde citral juice, d-taro alkene, γ-Song Youxi, capraldehyde, octanal, citral, normal butyl alcohol are joined in the container; Put electromagnetic wand in the liquid into, on electromagnetic oven, stirred 60 minutes.
A kind of application of aforesaid transparency of organization reagent, wherein, the application of said transparency of organization reagent is used for transparent animal, human body and clinical pathology tissue with said transparency of organization reagent.
The application of described transparency of organization reagent wherein, may further comprise the steps:
Get tissue sample after alcohol-pickled, put into described transparency of organization agent submergence 6 hours.
Beneficial effect: transparency of organization reagent of the present invention, effect is rapid, and can mix with alcohol, and dissolving paraffin can mix with the natural gum of Feng Zangyong again, has the effect of strong osmotic.The sample transparent effect of crossing through transparency of organization agent treated of the present invention is good, and alternative common agents xylene solves owing to use safety and the environmental issue that xylene brought.
Embodiment
For making the object of the invention, technical scheme and effect clearer, clear and definite, below to further explain of the present invention.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
The present invention provides a kind of transparency of organization reagent, substitutes the transparent chemical reagent of xylene, and its content is compound chemical constitution, has the special efficacy of strong osmotic, and, be Green Product.
Particularly, said transparency of organization reagent according to percent by volume, comprises following component:
A-firpene 0.59-0.79%;
B-firpene 0.45-0.65%;
Limonene 0.45-0.65%;
Terpenes 0.45-0.65%;
Longifolene 0.45-0.65%;
Carypohyllene 0.45-0.65%;
Aldehyde citral juice 0.45-0.65%;
D-taro alkene 32.3-34.3%;
γ-Song Youxi 26.7-28.7%;
Capraldehyde 0.45-0.65%;
Octanal 0.45-0.65%;
Citral 0.45-0.65%;
Normal butyl alcohol 32.3-34.3%.
Wherein, the molecular formula of said terpenes is C
30H
48, molecular weight is 408.The molecular formula of said capraldehyde is C
10H
20O, molecular weight: 156.26.The molecular formula of said aldehyde citral juice is C
10H
16O, molecular weight are 152.23.
Said normal butyl alcohol has another name called butanols, and molecular formula is C
4H
10O, structural formula are CH
3(CH
2)
3OH.
The preparation method of said transparency of organization reagent is provided among the present invention, and it specifically may further comprise the steps:
According to above-mentioned prescription, successively each component is joined in the container, put electromagnetic wand in the liquid into, stirring about 60 minutes on the electromagnetic oven, after whole dissolvings, can obtain this product.
Said transparency of organization reagent can be used for substituting the transparent chemical reagent of xylene, and its effect can mix with alcohol rapidly, and dissolving paraffin can mix with the natural gum of Feng Zangyong again.But said transparency of organization reagent must purify moisture in use, otherwise the emulsus muddiness can take place.For fear of the acute contraction of material, it is the said transparency of organization reagent in anhydrous alcohol → 1/2 anhydrous alcohol+1/2 → said transparency of organization reagent that said transparency of organization reagent should be taked progressively to carry out the transition to from absolute alcohol.
The application of said transparency of organization reagent is provided among the present invention, said transparency of organization reagent is used for transparent animal, human body and clinical pathology tissue.Said transparency of organization reagent can be used for substituting xylene hyaline tissue, has the effect that is better than xylene, and tissue is become fragile, and do not have toxic action again, be the environment-friendly and green product, human body is safe from harm.
Said transparency of organization reagent according to percent by volume, most preferably is and comprises following component:
A-firpene 0.69%;
B-firpene 0.55%;
Limonene 0.55%;
Terpenes 0.55%;
Longifolene 0.55%;
Carypohyllene 0.55%;
Aldehyde citral juice 0.55%;
D-taro alkene 33.3%;
γ-Song Youxi 27.7%;
Capraldehyde 0.55%;
Octanal 0.55%;
Citral 0.55%;
Normal butyl alcohol 33.3%.
Research through a large amount of experiment and inventor is allocated, and finds that the effect that the made transparency of organization reagent place of above-mentioned prescription reaches is ideal.After will organizing process alcohol transparent, put into said transparency of organization liquid about 6 hours, immerse then in the wax (65 degrees centigrade), confirm that through section transparent effect is best, is unlikely to make tissue to become fragile again, obtains optimal effect.
Below through concrete embodiment the present invention is done further explain:
Embodiment 1
Number percent by volume; Get a-firpene 25 ml, b-firpene 20 ml, limonene 20 ml, terpenes 20 ml, longifolene 20 ml, carypohyllene 20 ml, aldehyde citral juice 20 ml, d-taro alkene 1200 ml, γ-Song Youxi 1000 ml, capraldehyde 20 ml, octanal 20 ml, citral 20 ml, normal butyl alcohol 1200 ml; Successively each component is joined in the container; Put electromagnetic wand in the liquid into, on electromagnetic oven, stirred 60 minutes, after whole dissolvings, can obtain this product.
Said transparency of organization reagent processed process is: the said transparency of organization reagent in anhydrous alcohol → 1/2 anhydrous alcohol+1/2 → said transparency of organization reagent.
Get 22 kinds of 33 internal organs such as rat brain, thyroid gland, lung, liver and tissues, sample is put into described transparency of organization agent submergence 6 hours after alcohol-pickled, immerses then in 65 ℃ of wax liquid, last slicing treatment.Confirm that through section embodiment 1 income effect is ideal, described transparency of organization reagent can make the tissue sample of processing present fine transparent effect, and is unlikely to make tissue to become fragile.
Embodiment 2
Number percent by volume; Get a-firpene 15.8ml, b-firpene 9.2 ml, limonene 11ml, terpenes 11 ml, longifolene 11 ml, carypohyllene 11 ml, aldehyde citral juice 9.2ml, d-taro alkene 686ml, γ-Song Youxi 546ml, capraldehyde 9.2ml, octanal 11 ml, citral 9.2 ml, normal butyl alcohol 660.4ml; Successively each component is joined in the container; Put electromagnetic wand in the liquid into, on electromagnetic oven, stirred 60 minutes, after whole dissolvings, can obtain this product.
Said transparency of organization reagent processed process is: the said transparency of organization reagent in anhydrous alcohol → 1/2 anhydrous alcohol+1/2 → said transparency of organization reagent.
Get 20 internal organs such as rat brain, thyroid gland, lung, liver, sample is put into described transparency of organization agent submergence 6 hours after alcohol-pickled, immerses then in 65 ℃ of wax liquid, last slicing treatment.Confirm that through section described transparency of organization reagent can make the tissue sample of processing present fine transparent effect, and is unlikely to make tissue to become fragile.
Embodiment 3
Number percent by volume; Get a-firpene 11.8 ml, b-firpene 11 ml, limonene 9.2ml, terpenes 9.2 ml, longifolene 9.2ml, carypohyllene 9.2ml, aldehyde citral juice 11 ml, d-taro alkene 646 ml, γ-Song Youxi 574ml, capraldehyde 11ml, octanal 9.2ml, citral 11ml, normal butyl alcohol 680ml; Successively each component is joined in the container; Put electromagnetic wand in the liquid into, on electromagnetic oven, stirred 60 minutes, after whole dissolvings, can obtain this product.
Said transparency of organization reagent processed process is: the said transparency of organization reagent in anhydrous alcohol → 1/2 anhydrous alcohol+1/2 → said transparency of organization reagent.
Get 30 internal organs such as rat brain, thyroid gland, lung, liver, sample is put into described transparency of organization agent submergence 6 hours after alcohol-pickled, immerses then in 65 ℃ of wax liquid, last slicing treatment.Confirm that through section described transparency of organization reagent can make the tissue sample of processing present fine transparent effect, and is unlikely to make tissue to become fragile.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, concerning those of ordinary skills, can improve or conversion that all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (5)
1. a transparency of organization reagent is characterized in that, according to percent by volume, comprises following component:
A-firpene 0.59-0.79%;
B-firpene 0.45-0.65%;
Limonene 0.45-0.65%;
Terpenes 0.45-0.65%;
Longifolene 0.45-0.65%;
Carypohyllene 0.45-0.65%;
Aldehyde citral juice 0.45-0.65%;
D-taro alkene 32.3-34.3%;
γ-Song Youxi 26.7-28.7%;
Capraldehyde 0.45-0.65%;
Octanal 0.45-0.65%;
Citral 0.45-0.65%;
Normal butyl alcohol 32.3-34.3%.
2. transparency of organization reagent according to claim 1 is characterized in that, according to percent by volume, comprises following component:
A-firpene 0.69%;
B-firpene 0.55%;
Limonene 0.55%;
Terpenes 0.55%;
Longifolene 0.55%;
Carypohyllene 0.55%;
Aldehyde citral juice 0.55%;
D-taro alkene 33.3%;
γ-Song Youxi 27.7%;
Capraldehyde 0.55%;
Octanal 0.55%;
Citral 0.55%;
Normal butyl alcohol 33.3%.
3. the preparation method like each described transparency of organization reagent of claim 1-2 is characterized in that, may further comprise the steps:
Successively a-firpene, b-firpene, limonene, terpenes, longifolene, carypohyllene, aldehyde citral juice, d-taro alkene, γ-Song Youxi, capraldehyde, octanal, citral, normal butyl alcohol are joined in the container; Put electromagnetic wand in the liquid into, on electromagnetic oven, stirred 60 minutes.
4. the application like each described transparency of organization reagent of claim 1-2 is characterized in that, the application of said transparency of organization reagent is used for transparent animal, human body and clinical pathology tissue with said transparency of organization reagent.
5. the application of transparency of organization reagent according to claim 4 is characterized in that, may further comprise the steps:
Get tissue sample after alcohol-pickled, put into described transparency of organization agent submergence 6 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102672339A CN102749244A (en) | 2012-07-31 | 2012-07-31 | Tissue transparent reagent, and preparation method and applications thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102672339A CN102749244A (en) | 2012-07-31 | 2012-07-31 | Tissue transparent reagent, and preparation method and applications thereof |
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| Publication Number | Publication Date |
|---|---|
| CN102749244A true CN102749244A (en) | 2012-10-24 |
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ID=47029610
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102672339A Pending CN102749244A (en) | 2012-07-31 | 2012-07-31 | Tissue transparent reagent, and preparation method and applications thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146354A (en) * | 2019-05-14 | 2019-08-20 | 中国人民解放军第八一医院 | The new method of modified trimethylolpropane fatty acid ester dissolution paraffin |
-
2012
- 2012-07-31 CN CN2012102672339A patent/CN102749244A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146354A (en) * | 2019-05-14 | 2019-08-20 | 中国人民解放军第八一医院 | The new method of modified trimethylolpropane fatty acid ester dissolution paraffin |
| CN110146354B (en) * | 2019-05-14 | 2021-11-23 | 东部战区总医院秦淮医疗区 | Method for dissolving paraffin by modified trimethylolpropane fatty acid ester |
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Application publication date: 20121024 |