CN102747027A - Culture medium for producing large quantities of spores of aschersonia placenta - Google Patents
Culture medium for producing large quantities of spores of aschersonia placenta Download PDFInfo
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- CN102747027A CN102747027A CN2012102674457A CN201210267445A CN102747027A CN 102747027 A CN102747027 A CN 102747027A CN 2012102674457 A CN2012102674457 A CN 2012102674457A CN 201210267445 A CN201210267445 A CN 201210267445A CN 102747027 A CN102747027 A CN 102747027A
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- spore
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- seat shell
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Abstract
The invention relates to a culture medium for producing large quantities of spores of aschersonia placenta. The culture medium is obtained through the optimization of a response surface method and consists of 33.0-35.0g of millet, 2.90-3.10g of tryptone, 1.00-1.20g of dipotassium phosphate, 0.3-0.5g of magnesium sulfate, 20.0g of agar and 1L of water. The culture medium is favorable for eliminating the influences of the components in the culture medium on the attributes of a propagating body such as yield, biological control effect, drying tolerance and survival rate and the like, and is higher in spore yield; and the yield per unit of the spores is more than 1.774*10<10> spores/L, and is 10 times that of an equivalent PDA (potato dextrose agar) culture medium, and the yield per unit of the spores of the equivalent PDA culture medium is 1.792*10<9> spores/L. The culture medium provides a reference condition for large-scale batch production of the spores of the aschersonia placenta.
Description
Technical field
The present invention relates to a kind of big volume production spore of flat seat shell spore bacterium substratum that obtains through the optimization of response surface method.
Background technology
Flat seat shell spore bacterium (
Aschersonia placenta) be the important member of entomogenous fungi, its teleomorph for do not rein in bacterium (
Moelleriella), be under the jurisdiction of Ascomycota, caprophyl guiding principle, Hypocreales, Clavicipitaceae.Existing research shows that flat seat shell spore bacterium and teleomorph thereof do not rein in bacterium and can cause aleyrodid and the epiphytotics generation of shell class insect group, and this makes it become the effective biological control factor.And as the biological prevention and control factor, the spore of fungi is owing to survival time is grown, environmental resistance is easier to mass production and commercialization by force.Therefore this bacterium spore of mass production becomes vital problem.According to research, medium component to the attribute of propagulum such as output, give birth to control usefulness, exsiccant tolerance and survival rate or the like all had very remarkable influence, and there are differences because of the bacterial strain difference.
Summary of the invention
The object of the present invention is to provide the big volume production spore of a kind of flat seat shell spore bacterium substratum, this substratum help eliminating medium component to attribute such as the output of propagulum, give birth to control usefulness, to the influence of exsiccant tolerance and survival rate etc.
For realizing above-mentioned purpose, technical scheme of the present invention is: the big volume production spore of a kind of flat seat shell spore bacterium substratum, form by millet, Tryptones, potassium hydrogenphosphate, sal epsom and water; Millet 33.0 g～35.0g wherein; Tryptones 2.90 g～3.10g, potassium hydrogenphosphate 1.00 g～1.20g, sal epsom 0.3g～0.5g; Agar 20.0g, water is settled to 1L.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum is that solid produces the spore substratum.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum can be prepared by ordinary method, and sterilising conditions is: 121 ℃ of temperature, pressure range 0.1 MPa～0.15MPa, time 30min.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum need be cooled to it before inoculation and solidify.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum inoculum size when inoculation to be controlled at the substratum weight ratio 10% in.
In the big volume production spore of the above-mentioned flat seat shell spore bacterium substratum, carbon source is a millet, and nitrogenous source is a Tryptones, and its optimal proportion is: millet 33.8g, and Tryptones 3g, potassium hydrogenphosphate 1.11g, sal epsom 0.37g, agar 20.0g, water is settled to 1L.
The invention has the beneficial effects as follows through the optimization of response surface method and obtain the big volume production spore of flat seat shell spore bacterium substratum; Not only avoided medium component to attribute such as the output of propagulum, give birth to control usefulness, to the influence of exsiccant tolerance and survival rate etc.; And spore output is higher, and the unit output of spore can reach 1.774 * 10
10More than the spores/L, be equivalent PDA substratum output 1.792 * 10
910 times of spores/L.This substratum provides reference conditions for the large-scale mass production of this bacterium spore.
Description of drawings
Fig. 1 is the formation synoptic diagram of the big volume production spore of the present invention flat seat shell spore bacterium substratum.
Fig. 2 is through response surface method optimization substratum gained of the present invention millet (X
1) and K
2HPO
4(X
2) two factors are to the response surface chart of the influence of spore output.
Fig. 3 is through response surface method optimization substratum gained of the present invention millet (X
1) and K
2HPO
4(X
2) two factors are to the isogram of the influence of spore output.
Fig. 4 is through response surface method optimization substratum gained of the present invention millet (X
1) and MgSO
47H
2O (X
3) two factors are to the response surface chart of the influence of spore output.
Fig. 5 is through response surface method optimization substratum gained of the present invention millet (X
1) and MgSO
47H
2O (X
3) two factors are to the isogram of the influence of spore output.
Fig. 6 is through response surface method optimization substratum gained of the present invention K
2HPO
4(X
2) and MgSO
47H
2O (X
3) two factors are to the response surface chart of the influence of spore output.
Fig. 7 is through response surface method optimization substratum gained of the present invention K
2HPO
4(X
2) and MgSO
47H
2O (X
3) two factors are to the isogram of the influence of spore output.
Embodiment
The big volume production spore of flat seat shell spore bacterium of the present invention substratum; Form millet 33.0 g～35.0g wherein, Tryptones 2.90 g～3.10g by millet, Tryptones, potassium hydrogenphosphate, sal epsom and water; Potassium hydrogenphosphate 1.00 g～1.20g; Sal epsom 0.3g～0.5g, agar 20.0g, water is settled to 1L.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum is that solid produces the spore substratum.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum can be prepared by ordinary method, and sterilising conditions is: 121 ℃ of temperature, pressure range 0.1 MPa～0.15MPa, time 30min.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum need be cooled to it before inoculation and solidify.
The big volume production spore of above-mentioned flat seat shell spore bacterium substratum inoculum size when inoculation to be controlled at the substratum weight ratio 10% in.
In the big volume production spore of the above-mentioned flat seat shell spore bacterium substratum, carbon source is a millet, and nitrogenous source is a Tryptones, and its optimal proportion is: millet 33.8g, and Tryptones 3g, potassium hydrogenphosphate 1.11g, sal epsom 0.37g, agar 20.0g, water is settled to 1L.
Below in conjunction with specific embodiment process and the effect of utilizing this bacterium of the big volume production spore of flat seat shell spore bacterium of the present invention culture medium culturing to obtain spore is further described.
Embodiment 1: obtain this bacterium from the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium, carry out separation and purification, and by form and molecular biology evidence it is identified.It is seeded in above-mentioned substratum and the PDA control medium, carries out spore production according to ordinary method respectively.The result shows that the unit output of the spore of the big volume production spore of flat seat shell spore bacterium substratum can reach 1.774 * 10
10More than the spores/L, be equivalent PDB substratum output 1.692 * 10
9About 10 times of spores/L.Wherein, the big volume production spore of flat seat shell spore bacterium substratum is prepared by optimal proportion.
Embodiment 2: obtain this bacterium from the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium, carry out separation and purification, and by form and molecular biology evidence it is identified.It is seeded in above-mentioned substratum and the PDA control medium, carries out spore production according to ordinary method respectively.The result shows that the unit output of the spore of the big volume production spore of flat seat shell spore bacterium substratum can reach 1.534 * 10
10More than the spores/L, be equivalent PDB substratum output 1.528 * 10
9About 10 times of spores/L.Wherein, the big volume production spore of flat seat shell spore bacterium culture medium prescription is: millet 33.0g wherein, and Tryptones 2.95g, potassium hydrogenphosphate 1.20g, sal epsom 0.3g, agar 20.0g, water is settled to 1L.
Embodiment 3: obtain this bacterium from the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium, carry out separation and purification, and by form and molecular biology evidence it is identified.It is seeded in above-mentioned substratum and the PDA control medium, carries out spore production according to ordinary method respectively.The result shows that the unit output of the spore of the big volume production spore of flat seat shell spore bacterium substratum can reach 1.657 * 10
10More than the spores/L, be equivalent PDB substratum output 1.643 * 10
9About 10 times of spores/L.Wherein, the big volume production spore of flat seat shell spore bacterium culture medium prescription is: millet 34.5g wherein, and Tryptones 3.10g, potassium hydrogenphosphate 1.00g, sal epsom 0.3g, agar 20.0g, water is settled to 1L.
More than be preferred embodiment of the present invention, all changes of doing according to technical scheme of the present invention when the function that is produced does not exceed the scope of technical scheme of the present invention, all belong to protection scope of the present invention.
Claims (7)
1. the big volume production spore of flat seat shell spore bacterium substratum; It is characterized in that: form millet 33.0 g～35.0g wherein, Tryptones 2.90 g～3.10g by millet, Tryptones, potassium hydrogenphosphate, sal epsom and water; Potassium hydrogenphosphate 1.00 g～1.20g; Sal epsom 0.3g～0.5g, agar 20.0g, water is settled to 1L.
2. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: the big volume production spore of said flat seat shell spore bacterium substratum is that solid produces the spore substratum.
3. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: the sterilising conditions of the big volume production spore of said flat seat shell spore bacterium substratum is: 121 ℃ of temperature, pressure range 0.1 MPa～0.15MPa, time 30min.
4. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: the big volume production spore of said flat seat shell spore bacterium substratum need be cooled to it before inoculation and solidify.
5. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: the big volume production spore of said flat seat shell spore bacterium substratum inoculum size when inoculation to be controlled at the substratum weight ratio 10% in.
6. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: in the big volume production spore of the said flat seat shell spore bacterium substratum, carbon source is a millet; Nitrogenous source is a Tryptones, and its optimal proportion is: millet 33.8g, Tryptones 3g; Potassium hydrogenphosphate 1.11g; Sal epsom 0.37g, agar 20.0g, water is settled to 1L.
7. the big volume production spore of a kind of flat seat shell spore bacterium according to claim 1 substratum is characterized in that: the big volume production spore of said flat seat shell spore bacterium substratum obtains through the optimization of response surface method.
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| CN201210267445.7A CN102747027B (en) | 2012-07-31 | 2012-07-31 | Culture medium for producing large quantities of spores of aschersonia placenta |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103014066A (en) * | 2012-12-03 | 2013-04-03 | 福建农林大学 | Extraction method of aschersonia secondary metabolite |
| CN103275950A (en) * | 2013-06-09 | 2013-09-04 | 福建农林大学 | Culture medium and method for producing lipase by aschersonia placenta fermentation |
| CN104498366A (en) * | 2014-11-19 | 2015-04-08 | 华中农业大学 | Large-scale production and fermentation culture medium of aschersonia placenta and fermentation cultivation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250489A (en) * | 2008-03-22 | 2008-08-27 | 杨毅 | Industrial method for artificially cultivating Chinese caterpillar fungus |
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2012
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250489A (en) * | 2008-03-22 | 2008-08-27 | 杨毅 | Industrial method for artificially cultivating Chinese caterpillar fungus |
Non-Patent Citations (2)
| Title |
|---|
| 王震: "扁座壳孢菌生理特性及乳悬剂的初步研究", 《福建农林大学硕士学位论文》 * |
| 邱君志: "粉虱座壳孢培养基的筛选试验", 《河南科技大学学报：自然科学版》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103014066A (en) * | 2012-12-03 | 2013-04-03 | 福建农林大学 | Extraction method of aschersonia secondary metabolite |
| CN103275950A (en) * | 2013-06-09 | 2013-09-04 | 福建农林大学 | Culture medium and method for producing lipase by aschersonia placenta fermentation |
| CN104498366A (en) * | 2014-11-19 | 2015-04-08 | 华中农业大学 | Large-scale production and fermentation culture medium of aschersonia placenta and fermentation cultivation method |
| CN104498366B (en) * | 2014-11-19 | 2017-03-29 | 华中农业大学 | A kind of large-scale production fermentation medium of flat Aschersonia and fermentation culture method |
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| CN102747027B (en) | 2014-11-19 |
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