CN102730909B - Preparation method of seawater for field shellfish unicellular alga culture - Google Patents
Preparation method of seawater for field shellfish unicellular alga culture Download PDFInfo
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- CN102730909B CN102730909B CN201210208233.1A CN201210208233A CN102730909B CN 102730909 B CN102730909 B CN 102730909B CN 201210208233 A CN201210208233 A CN 201210208233A CN 102730909 B CN102730909 B CN 102730909B
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- seawater
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- micro algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a preparation method of seawater for field shellfish unicellular alga culture. The preparation method comprises the steps of seawater precipitation, filter feeding shellfish filter, absorbent cotton filter and disinfection. The preparation method, which utilizes particulate substance filter physiological characteristics of seawater bivalve shellfish and allows filter materials of a nylon net, the absorbent cotton and the like to be cooperatively used, enables a large amount of clear seawater to be prepared without a sand filter tank, so the preparation method has the advantages of low cost and convenient operation, can be widely applied to field experiments or small-scale seedling production, and has a good application prospect.
Description
Technical field
The invention belongs to the cultivation field of field shellfish Micro Algae, particularly a kind of field shellfish Micro Algae is cultivated the preparation method with seawater.
Background technology
Unicellular algae is the high-quality bait of filter-feeding shellfish, is widely used in shellfish hatchery and cultivation.At present, the culture technique of unit cell algae is ripe, and one will carry out three grades of cultivations, protects expanding species cultivation, enlarged culturing and productive culture that is:.During Micro Algae is cultivated, except illumination, nutritive salt and temperature, the processing of water is a very important link.Filtration and sterilization comprising water.Conservation water generally need be through secondary sand filtration, and then filters through gac or absorbent cotton, and then carries out scalding or sterilized water sterilization.In algae enlarged culturing and productive culture, seawater used also needs through sand filtration.But, in small-scale shellfish hatchery or scientific research and testing in the wild, be subject to place or condition restriction, often cannot obtain sand filtration seawater.If alone absorbent cotton or activated carbon filtration are not only consuming time, the gained water yield is limited, and absorbent cotton and gac need to change at any time, and cumbersome, cost is also higher.In addition, its filter effect is also undesirable, and especially for mud type mudflat seawater, after filtering, water body is containing a large amount of suspended particulates, and light transmission is poor, and the growth of Micro Algae is cultivated in impact.
Filter-feeding marine shellfish can be filtered plant plankton and the particulate matter in food picked-up seawater, and filtration rate is high, Zhang Jihong etc. (2005) report, and the seawater amount of single chlamys farreri filtration per hour is up to 4L.Only selective to the size of food and quality when filter-feeding shellfish ingests, and to whether having edibleness there is no selectivity.Therefore, the particle in certain particle size range that filter-feeding shellfish suspends in can filtering sea, and the false excrement of it formation or ight soil are entered in substrate.According to
(1988) research report, bivalve shellfish is all filterings of particle more than 4-5 μ m to particle diameter; Particle to particle diameter 2 μ m, its rejection rate also can reach 50%.And according to the research report of (2007) such as Li Zhanhai, the outstanding husky particle diameter compositing range of tidal flat is between 3-22 μ m.Therefore, this Feeding Physiology characteristic of bivalve filter food shellfish can be used.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of field shellfish Micro Algae and cultivates the preparation method with seawater, the method is utilized the physiological property of seawater bivalve shellfish filter particulate material, coordinate the filtering materials such as nylon wire, absorbent cotton, without sand filter, can prepare a considerable amount of compared with ten clean seawater, cost is low, easy and simple to handle, can be widely used in field trial or the production of growing seedlings on a small scale, have a good application prospect.
A kind of field of the present invention shellfish Micro Algae is cultivated the preparation method with seawater, comprising:
(1) seawater extracting is precipitated to filtration vessel, filtration vessel top hides with black cloth, and sedimentation time is 24～28 hours; On extraction settling tank, part seawater is to filtration vessel, and pump outlet carries out prefiltration with nylon net bag to seawater;
(2) select healthy filter-feeding shellfish and be placed in net, then put into filtration vessel bottom, filtration vessel hides with black cloth, every sampling observation in 6 hours, to estimating water body clarification, sees the bottom; Filter completely, with flexible pipe siphon, remove shellfish ight soil and the settling on bottom and net cage;
(3) seawater of cultivating for Micro Algae conservation is carried out to scalding, to the seawater for Micro Algae enlarged culturing and productive culture, use sodium hypochlorite solution or chlorinated lime to carry out disinfection; After sterilization, inflate after 12 ~ 16 hours with inoculating with the rear Micro Algae that can be used in Sulfothiorine.
Filtration vessel in described step (1) is indoor settling tank or Glass fibre reinforced plastic tub.
The order number of the nylon net bag in described step (1) is 150-200 order.
Filter-feeding shellfish in described step (2) is clam.
In described step (3), to using absorbent cotton filtering sea before the seawater scalding of cultivating for Micro Algae conservation, remove large granular impurity.
Described absorbent cotton filters concrete technology: the absorbent cotton of thickness 2-3cm is laid in Büchner funnel, with small hose siphon seawater, to funnel, filters.
The concentration of the Sulfothiorine in described step (3) is 25ppm.
beneficial effect
The present invention utilizes the physiological property of seawater bivalve shellfish filter particulate material, coordinate the filtering materials such as nylon wire, absorbent cotton, without sand filter, can prepare a considerable amount of cleaner seawater, cost is low, easy and simple to handle, can be widely used in field trial or the production of growing seedlings on a small scale, have a good application prospect.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
Conservation is cultivated:
1, seawater precipitation
Seawater used is taken from the outdoor water reservoir of 100m * 100m, with compact submersible pump by seawater extracting to indoor cement settling tank, the 1.5m of the long 4m of settling tank specification * wide 3m * deeply, water pump water outlet mouthpiece cover 150 object nylon net bags carry out prefiltration, add water to 1.2m.Settling tank black cloth shading, precipitated after 24 hours, and submersible pump is placed in a filtration net cage, and net cage is put into settling tank, by the white plastic tank of 2 100L of seawater suction, and the high 1m of bucket.
2, filter-feeding shellfish is filtered
Buy clam 5kg left and right common on market, the average long 4.5cm of shell, cleans shell, on average puts into 2 plastics string bags, then puts into respectively white plastic tank.In bucket, inflation, hides with black cloth.Every sampling in 4 hours, observe, with flexible pipe siphon, remove the bucket end, online shellfish ight soil and settling simultaneously; After 12 hours, the visible bucket of the range estimation end, the plastics string bag is taken out.
3, absorbent cotton filters
Get one of Büchner funnel (upper bore 150mm, bore 3cm lets slip a remark), absorbent cotton is layered in funnel layer by layer, compress, to thickness 3cm, both sides spread to funnel edge; The with cover round plastic tank of 50L, top cover boring, aperture 3.5 – 4cm.Plastic cylinder is placed on to lower, and funnel is placed in the hole of bung, with small hose siphon seawater in above-mentioned white plastic tank, to funnel, filters, and with clip, controls water flow velocity with anti-spilled, to bucket, completely can stop filtering.
4, seawater sterilization
Get the Erlenmeyer flask of 5000mL, with 1:1 hydrochloric acid, scrub, after rinsing with fresh water, add the seawater of absorbent cotton filtration to 4500mL.After boiling with 1500W electric furnace, with double-deck printer paper sealing, and tighten with bungee.Can be used for after cooling inoculation.
5, inoculation culture
Isochrysis galbana and each 500mL of Chlorella pyrenoidesa algae kind are accessed in 4500 seawater that scalding is good, add respectively nutrient salt solution, be placed in indoor cultivation, adopt natural lighting, room temperature 26-33 ℃.Shake bottle every day 4 times.To the 6th day, algae liquid concentration reached respectively 2.3 * 10
6individual/ml and 2.5 * 10
6individual/ml.And two kinds of algae colors that only cultivate with absorbent cotton filtering sea the same period are obviously lighter, mean concns is respectively 1.4 * 10
6individual/ml and 1.5 * 10
6individual/ml, and poor growth, wherein chrysophyceae is influenced particularly evident.
Embodiment 2
Enlarged culturing:
1, seawater extracting and precipitation
Seawater used is taken from the outdoor water reservoir of 100m * 100m, with compact submersible pump by seawater extracting to indoor cement settling tank, the 1.5m of the long 4m of settling tank specification * wide 3m * deeply, water pump water outlet mouthpiece cover 200 object nylon net bags carry out prefiltration, add water to 1.2m.Settling tank black cloth shading, precipitated after 28 hours, and submersible pump is placed in a filtration net cage, and net cage is put into one jiao of settling tank, by the white plastic cylinder of 15 100L of seawater suction, and the high 1m of bucket.
2, filter-feeding shellfish is filtered
Buy clam, the average long 4.5cm of shell, cleans shell, puts into the plastics string bag, puts into respectively each white plastic tank, puts 2.5kg left and right for every barrel.In bucket, inflation, hides with black cloth; Every sampling in 4 hours, observe, with flexible pipe siphon, remove the bucket end, online shellfish ight soil and settling simultaneously.After 12 hours, the visible bucket of the range estimation water body end, the plastics string bag is taken out, now clam can be used for sea water filter next time.
3, seawater sterilization
Sodium hypochlorite solution (available chlorine 15-30%) is splashed in bucket and carried out disinfection by the concentration of 250ppm, in bucket, inflation is uniformly distributed thimerosal, the transparent plastic film of a 1m * 1m is put into bucket sterilization simultaneously, after 16 hours, add Sulfothiorine neutralization, concentration is controlled at 25ppm left and right, after stirring, inflating, gets final product inoculation culture.
4, inoculation culture
The chrysophyceae of conservation and chlorella are accessed in the seawater disinfecting in the ratio of 1:8, added respectively nutrient salt solution, with the transparent plastic film of having sterilized, cover bung, carry out enlarged culturing.During cultivation, with the micro-inflation of independent air pump or stir every day 3 times.Under the condition of illumination abundance, after 5 days, algae liquid concentration all reaches 2.0 * 10
6individual/more than ml, to can be used for testing throwing something and feeding of shellfish.
Claims (6)
1. field shellfish Micro Algae is cultivated the preparation method with seawater, comprising:
(1) seawater extracting is precipitated to filtration vessel, filtration vessel top hides with black cloth, and sedimentation time is 24～28 hours; On extraction settling tank, part seawater is to filtration vessel, and pump outlet carries out prefiltration with nylon net bag to seawater; Wherein, the order number of nylon net bag is 150-200 order;
(2) select healthy filter-feeding shellfish and be placed in net, then put into filtration vessel bottom, filtration vessel hides with black cloth, every sampling observation in 6 hours, to estimating water body clarification, sees the bottom; Filter completely, with flexible pipe siphon, remove shellfish ight soil and the settling on bottom and net cage;
(3) seawater of cultivating for Micro Algae conservation is carried out to scalding, to the seawater for Micro Algae enlarged culturing and productive culture, use sodium hypochlorite solution or chlorinated lime to carry out disinfection; After sterilization, inflate after 12～16 hours with inoculating with the rear Micro Algae that can be used in Sulfothiorine; Wherein, described Micro Algae is Isochrysis galbana and Chlorella pyrenoidesa; Described seawater is cultivated and enlarged culturing for conservation.
2. a kind of field according to claim 1 shellfish Micro Algae is cultivated the preparation method with seawater, it is characterized in that: the filtration vessel in described step (1) is indoor settling tank or Glass fibre reinforced plastic tub.
3. a kind of field according to claim 1 shellfish Micro Algae is cultivated the preparation method with seawater, it is characterized in that: the filter-feeding shellfish in described step (2) is clam.
4. a kind of field according to claim 1 shellfish Micro Algae is cultivated the preparation method with seawater, it is characterized in that: in described step (3), to using absorbent cotton filtering sea before the seawater scalding of cultivating for Micro Algae conservation, remove large granular impurity.
5. a kind of field according to claim 1 shellfish Micro Algae is cultivated the preparation method with seawater, it is characterized in that: described absorbent cotton filters concrete technology and is: the absorbent cotton of thickness 2-3cm is laid in Büchner funnel, with small hose siphon seawater, to funnel, filters.
6. a kind of field according to claim 1 shellfish Micro Algae is cultivated the preparation method with seawater, it is characterized in that: the concentration of the Sulfothiorine in described step (3) is 25ppm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210208233.1A CN102730909B (en) | 2012-06-21 | 2012-06-21 | Preparation method of seawater for field shellfish unicellular alga culture |
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| CN201210208233.1A CN102730909B (en) | 2012-06-21 | 2012-06-21 | Preparation method of seawater for field shellfish unicellular alga culture |
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| CN102730909A CN102730909A (en) | 2012-10-17 |
| CN102730909B true CN102730909B (en) | 2014-12-10 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104872045A (en) * | 2015-06-01 | 2015-09-02 | 邹苏琪 | Fully-automatic juvenile fish breeding device |
| CN108668965A (en) * | 2018-03-22 | 2018-10-19 | 广东海洋大学 | A kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal |
| CN109336285A (en) * | 2018-10-23 | 2019-02-15 | 江苏省海洋水产研究所 | A kind of easy preparation device and its application method of microalgae conservation culture water |
| CN109796092A (en) * | 2019-02-03 | 2019-05-24 | 福安市冠富农业科技有限公司 | A kind of method for purifying seawater |
| CN109769685A (en) * | 2019-02-03 | 2019-05-21 | 福安市冠富农业科技有限公司 | A kind of culture zone scene spreads cultivation algae method |
| CN113080051B (en) * | 2021-03-26 | 2023-10-20 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
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| CN101156558A (en) * | 2007-10-19 | 2008-04-09 | 中国水产科学研究院南海水产研究所 | large nacre artificial sprout continental rise controlled water body intermediate cultivate method |
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Granted publication date: 20141210 Termination date: 20180621 |