CN102726371A - Long-acting boar semen diluting powder and preparation method thereof - Google Patents
Long-acting boar semen diluting powder and preparation method thereof Download PDFInfo
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- CN102726371A CN102726371A CN2012102385014A CN201210238501A CN102726371A CN 102726371 A CN102726371 A CN 102726371A CN 2012102385014 A CN2012102385014 A CN 2012102385014A CN 201210238501 A CN201210238501 A CN 201210238501A CN 102726371 A CN102726371 A CN 102726371A
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Abstract
The invention provides a preparation method of long-acting boar semen diluting powder. According to the method, 26-36 g of glucose, 3-7 g of dry chicken egg-yolk powder, 3-6 g of sodium citrate, 1.5-2.5 g of aminoacetic acid, 1.0-2.0 g of disodium hydrogen phosphate, 1.0-2.0 g of ethylene diamine tetraacetic acid disodium, 2.0-4.0 g of potassium sorbate, 1.0-2.0 g of calcium propionate, and 0.005-0.02 g of gentamicin powder are taken; the powdery raw materials taken according to the previous quantities are evenly mixed together so that the boar semen diluting powder is obtained. The preparation method of the dry chicken egg-yolk powder comprises the following steps: cooking a fresh chicken egg, taking out the egg yolk and drying the egg yolk to obtain dry chicken egg-yolk powder with the moisture content of 1-10%. The nutrient solution for diluting boar semen prepared by the raw materials provided in the method and distilled water contains a nutritional agent, a diluting agent, a buffering agent, a pH regulator and an antibiotic, as well as a preservative and a bacteriostatic agent, and can be preserved for 0-12 days under the condition of a constant temperature ranging from 15 to 20 DEG C.
Description
Technical field
The present invention relates to a kind of diluting boar semen powder that the diluting boar semen agent uses and preparation method thereof, particularly a kind of composition of diluting boar semen powder prepared.
Background technology
Boar is in the seminal fluid of once ejaculation, and the number of sperm that comprises is far away more than the required quantity of sow fertilization.In the artificial insemination process of pig; The nutrient solution of available energy dilution pig seminal fluid is with many times of diluting boar semens; Pig seminal fluid after the dilution has not only increased capacity, also can make pig seminal fluid ground during a fixed length preserve to get up to use, so that be more sow semen deposition; Thereby improve the breeding efficiency of stock boar, improve the reproduction rate of outstanding stock boar greatly.
The nutrient solution that can be mixed with diluting boar semen powder and distilled water during dilution pig seminal fluid.The diluting boar semen powder is a powdery product, is formed by the dusty raw materials mixed preparing.Nutritious dose, thinner, buffer, Ph conditioning agent and antibiotic in the composition of general diluting boar semen powder; Nutritional agents is generally used glucose, fructose etc.; The sodium chloride that thinner generally oozes with grade etc.; Buffer is generally used sodium citrate, sodium hydrogen phosphate, potassium dihydrogen phosphate etc., and the Ph conditioning agent is generally used disodium salt sodium etc., and antibiotic is generally used antibiotic such as penicillin, streptomycin, gentamicin.With the pig seminal fluid after the dilution of above-mentioned nutrient solution need be that 15-20 ℃ constant temperature is preserved in temperature.
In the prior art; With the pig seminal fluid after the dilution of above-mentioned nutrient solution is that the time of preservation is not very long down for 15-20 ℃ constant temperature in temperature; Generally be 5 ~ 7 days, the pig seminal fluid after the nutrient solution dilution that is mixed with this diluting boar semen powder is not suitable for requiring long use occasion to use in the holding time to the pig seminal fluid.
Summary of the invention
The objective of the invention is in order to overcome above-mentioned deficiency, proposing a kind of is long long-acting diluting boar semen powder of time of preserving under 15-20 ℃ the constant temperature and preparation method thereof in temperature.
The present invention realizes with following method.
Long-acting diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder with the amount of each composition is: powdered glucose: 26～36 grams, chicken with yolk dry powder: 3.0～7.0 grams; Sodium citrate: 3.0～6.0 grams, amion acetic acid: 1.5～2.5 grams, sodium hydrogen phosphate: 1.0～2.0 grams; Disodium salt disodium: 1.0～2.0 grams; Potassium sorbate: 2.0～4.0 grams, calcium propionate: 1.0～2.0 grams, gentamicin powder 0.005～0.02 gram.
The preparation method of diluting boar semen powder has the following steps:
1. get powdered glucose respectively: 26～36 grams; Sodium citrate: 3.0～6.0 grams, amion acetic acid: 1.5～2.5 grams, sodium hydrogen phosphate: 1.0～2.0 grams; Disodium salt disodium: 1.0～2.0 grams; Potassium sorbate: 2.0～4.0 grams, calcium propionate: 1.0～2.0 grams, gentamicin powder 0.005～0.02 gram;
2. preparation chicken with yolk dry powder, the yolk process for preparation of dry powder is: get fresh ovum gallinaceum, boil fresh ovum gallinaceum, the fresh chicken with yolk that taking-up is boiled is dried to the chicken with yolk dry powder of water content 7% ~ 10% to chicken with yolk, obtains chicken with yolk dry powder,
3. get above-mentioned chicken with yolk dry powder 3.0～7.0 grams;
4. the above-mentioned powdered glucose that takes out according to quantity, chicken with yolk dry powder, sodium citrate, amion acetic acid, sodium hydrogen phosphate, the disodium salt disodium, potassium sorbate, calcium propionate and gentamicin powder mix, and obtain the diluting boar semen powder.
The diluting boar semen powder can adopt and pack storage, and the pig of above-mentioned amount can be packaged into a bag with the sperm dilution powder.After product of the present invention adopts and packs, preserve, under 5～20 ℃ of conditions, can preserve 1 year, under 0～-10 ℃ of condition, can preserve 2 years in shady and cool dry place sealing,
During use,
1. an above-mentioned bag diluting boar semen powder is added in 1000 ml distilled waters, be preheating to 33～35 ℃, shake gently and make it whole dissolvings, become nutrient solution;
2. gather the pig seminal fluid, be placed on the pig seminal fluid in the container, nutrient solution is slowly poured in the pig seminal fluid that has just collected along container inner wall, the weight ratio that makes pig seminal fluid and nutrient solution is 1 ︰ 2, becomes diluting boar semen liquid for the first time;
3. diluting boar semen liquid left standstill 10 minutes for the first time;
4. add nutrient solution in the first time again in the diluting boar semen liquid, carry out the dilution of required multiple for the second time, let the ratio of boar semen and nutrient solution be 1 ︰ 3～10, become the diluting boar semen liquid second time;
5. the handle diluting boar semen liquid second time packs by 50～80 milliliters every part, under 15～20 ℃ temperature, stores.
50～80 milliliters every part the diluting boar semen liquid second time promptly is to an amount that the sow semen deposition is used.
Outside not only nutritious dose, thinner, buffer, Ph conditioning agent and the antibiotic, also have potassium sorbate preservative and bacteriostatic agent calcium propionate in addition in the nutrient solution of using with the dilution pig seminal fluid of the present invention preparation.Be under 15-20 ℃ the constant temperature with the pig seminal fluid of the nutrient solution dilution of the present invention preparation in temperature; Can preserve 10 ~ 12 days; Compare with the nutrient solution of diluting boar semen powder preparation of the prior art; Holding time is improved nearly one times, and the holding time that can satisfy the pig seminal fluid requires long use occasion to use.
Below in conjunction with embodiment, the present invention is done explanation further.
Embodiment
Embodiment 1
Long-acting diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder with the amount of each composition is: powdered glucose: 31 grams, sodium citrate: 4.0 grams; Amion acetic acid: 2.0 grams, sodium hydrogen phosphate: 2.0 grams, disodium salt disodium: 1.5 grams; Potassium sorbate: 3.0 grams; Calcium propionate: 1.5 grams, gentamicin powder 0.01 gram, chicken with yolk dry powder 5.0 grams;
Embodiment 2
The diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder with the amount of each composition is: powdered glucose: 27 grams, sodium citrate: 5.5 grams; Amion acetic acid: 1.5 grams, sodium hydrogen phosphate: 1.0 grams, disodium salt disodium: 2.0 grams; Potassium sorbate: 2.5 grams; Calcium propionate: 1.0 grams, gentamicin powder 0.02 gram, chicken with yolk dry powder 6.5 grams;
Embodiment 3
The diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder with the amount of each composition is: powdered glucose: 34 grams, sodium citrate: 3.5 grams; Amion acetic acid: 2.5 grams, sodium hydrogen phosphate: 1.5 grams, disodium salt disodium: 1.0 grams; Potassium sorbate: 2.0 grams; Calcium propionate: 2.0 grams, gentamicin powder 0.015 gram, chicken with yolk dry powder 3.0 grams;
Embodiment 4
The preparation method of diluting boar semen powder has the following steps:
1. get powdered glucose respectively: 31 grams, sodium citrate: 4.0 grams, amion acetic acid: 2.0 grams, sodium hydrogen phosphate: 2.0 grams, disodium salt disodium: 1.5 grams, potassium sorbate: 3.0 grams, calcium propionate: 1.5 grams, gentamicin powder 0.01 gram;
2. preparation chicken with yolk dry powder, the yolk process for preparation of dry powder is: get fresh ovum gallinaceum, boil fresh ovum gallinaceum, the fresh chicken with yolk that taking-up is boiled is dried to the chicken with yolk dry powder of water content 7% ~ 10% to chicken with yolk, obtains chicken with yolk dry powder,
3. get above-mentioned chicken with yolk dry powder 5.0 grams;
4. the above-mentioned powdered glucose that takes out according to quantity, chicken with yolk dry powder, sodium citrate, amion acetic acid, sodium hydrogen phosphate, the disodium salt disodium, potassium sorbate, calcium propionate and gentamicin powder mix, and obtain the diluting boar semen powder.
Embodiment 5
Get powdered glucose respectively: 27 grams, sodium citrate: 5.5 grams, amion acetic acid: 1.5 grams, sodium hydrogen phosphate: 1.0 grams, disodium salt disodium: 2.0 grams, potassium sorbate: 2.5 grams, calcium propionate: 2.0 grams, gentamicin powder 0.02 gram; Get above-mentioned chicken with yolk dry powder 6.5 grams;
Other is with embodiment 4.
Embodiment 6
Get powdered glucose respectively: 34 grams, sodium citrate: 3.5 grams, amion acetic acid: 2.5 grams, sodium hydrogen phosphate: 1.5 grams, disodium salt disodium: 1.0 grams, potassium sorbate: 2.0 grams, calcium propionate: 2.0 grams, gentamicin powder 0.015 gram; Get above-mentioned chicken with yolk dry powder 3.0 grams;
Other is with embodiment 4.
Claims (8)
1. a long-acting diluting boar semen powder is a powdery product, it is characterized in that: the composition of diluting boar semen powder with the amount of each composition is: powdered glucose: 26～36 grams; Chicken with yolk dry powder: 3.0～7.0 grams, sodium citrate: 3.0～6.0 grams, amion acetic acid: 1.5～2.5 grams; Sodium hydrogen phosphate: 1.0～2.0 grams, disodium salt disodium: 1.0～2.0 grams, potassium sorbate: 2.0～4.0 grams; Calcium propionate: 1.0～2.0 grams, gentamicin powder 0.005～0.02 gram.
2. by the described long-acting diluting boar semen powder of claim 1, it is characterized in that: the composition of described diluting boar semen powder with the amount of each composition is: powdered glucose: 31 grams, sodium citrate: 4.0 grams; Amion acetic acid: 2.0 grams, sodium hydrogen phosphate: 2.0 grams, disodium salt disodium: 1.5 grams; Potassium sorbate: 3.0 grams; Calcium propionate: 1.5 grams, gentamicin powder 0.01 gram, chicken with yolk dry powder 5.0 grams.
3. by the described long-acting diluting boar semen powder of claim 1, it is characterized in that: the composition of described diluting boar semen powder with the amount of each composition is: powdered glucose: 27 grams, sodium citrate: 5.5 grams; Amion acetic acid: 1.5 grams, sodium hydrogen phosphate: 1.0 grams, disodium salt disodium: 2.0 grams; Potassium sorbate: 2.5 grams; Calcium propionate: 1.0 grams, gentamicin powder 0.02 gram, chicken with yolk dry powder 6.5 grams.
4. by the described long-acting diluting boar semen powder of claim 1, it is characterized in that: the composition of described diluting boar semen powder with the amount of each composition is: powdered glucose: 34 grams, sodium citrate: 3.5 grams; Amion acetic acid: 2.5 grams; Sodium hydrogen phosphate: 1.5 grams, disodium salt disodium: 1.0 grams, potassium sorbate: 2.0 grams; Calcium propionate: 2.0 grams, gentamicin powder 0.015 gram; Chicken with yolk dry powder 3.0 grams.
5. preparation method by the described long-acting diluting boar semen powder of claim 1 is characterized in that: have the following steps:
⑴ get powdered glucose respectively: 26～36 grams; Sodium citrate: 3.0～6.0 grams, amion acetic acid: 1.5～2.5 grams, sodium hydrogen phosphate: 1.0～2.0 grams; Disodium salt disodium: 1.0～2.0 grams; Potassium sorbate: 2.0～4.0 grams, calcium propionate: 1.0～2.0 grams, gentamicin powder 0.005～0.02 gram;
⑵ prepare chicken with yolk dry powder, and the yolk process for preparation of dry powder is: get fresh ovum gallinaceum, boil fresh ovum gallinaceum, take out the fresh chicken with yolk boil, be dried to the chicken with yolk dry powder of water content 7% ~ 10% to chicken with yolk, obtain chicken with yolk dry powder,
⑶ get above-mentioned chicken with yolk dry powder 3.0～7.0 grams;
⑷ the above-mentioned powdered glucose that takes out according to quantity, chicken with yolk dry powder, and sodium citrate, amion acetic acid, sodium hydrogen phosphate, the disodium salt disodium, potassium sorbate, calcium propionate and gentamicin powder mix, and obtain the diluting boar semen powder.
6. by the preparation method of the described long-acting diluting boar semen powder of claim 5, it is characterized in that: described powdered glucose: 31 grams, sodium citrate: 4.0 grams; Amion acetic acid: 2.0 grams, sodium hydrogen phosphate: 2.0 grams, disodium salt disodium: 1.5 grams; Potassium sorbate: 3.0 grams; Calcium propionate: 1.5 grams, gentamicin powder 0.01 gram, chicken with yolk dry powder 5.0 grams.
7. by the preparation method of the described long-acting diluting boar semen powder of claim 5, it is characterized in that: described powdered glucose: 27 grams, sodium citrate: 5.5 grams; Amion acetic acid: 1.5 grams, sodium hydrogen phosphate: 1.0 grams, disodium salt disodium: 2.0 grams; Potassium sorbate: 2.5 grams; Calcium propionate: 1.0 grams, gentamicin powder 0.02 gram, chicken with yolk dry powder 6.5 grams.
8. by the preparation method of the described long-acting diluting boar semen powder of claim 5, it is characterized in that: described powdered glucose: 34 grams, sodium citrate: 3.5 grams; Amion acetic acid: 2.5 grams; Sodium hydrogen phosphate: 1.5 grams, disodium salt disodium: 1.0 grams, potassium sorbate: 2.0 grams; Calcium propionate: 2.0 grams, gentamicin powder 0.015 gram; Chicken with yolk dry powder 3.0 grams.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532645A (en) * | 2016-01-18 | 2016-05-04 | 赵彪 | Animal semen culture agent achieving constant-temperature preservation |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
| CN108077244A (en) * | 2018-01-31 | 2018-05-29 | 于文龙 | A kind of long-acting boar semen dilution powder |
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
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| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
| CN101796945A (en) * | 2010-04-12 | 2010-08-11 | 西北农林科技大学 | Antifreeze used for freezing and preserving boar semens as well as preparation and application thereof |
| CN101869101A (en) * | 2010-07-02 | 2010-10-27 | 广西大学 | Pig seminal fluid cryopreservation method |
| CN101965830A (en) * | 2010-10-18 | 2011-02-09 | 西北农林科技大学 | Anti-freezing agent and anti-freezing diluent for freezing and storing livestock sperm and preparation method thereof |
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| WO2002043486A1 (en) * | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532645A (en) * | 2016-01-18 | 2016-05-04 | 赵彪 | Animal semen culture agent achieving constant-temperature preservation |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
| CN108077244A (en) * | 2018-01-31 | 2018-05-29 | 于文龙 | A kind of long-acting boar semen dilution powder |
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
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Application publication date: 20121017 |