CN102721680A - Method for high-sensitivity detection for t-DNA (transfer-deoxyribose nucleic acid) by aid of SERS (surface enhanced Raman spectroscopy) liquid chip - Google Patents
Method for high-sensitivity detection for t-DNA (transfer-deoxyribose nucleic acid) by aid of SERS (surface enhanced Raman spectroscopy) liquid chip Download PDFInfo
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Abstract
The invention belongs to the technical field of biological molecule detection, and particularly discloses a method for high-sensitivity detection for t-DNA (transfer-deoxyribose nucleic acid) by the aid of an SERS (surface enhanced Raman spectroscopy) liquid chip. In the method, surface enhanced Raman spectroscopy (SERS) is used for coding, and the SERS liquid chip with magnetic composite nanoparticles as a capturing substrate is used for detecting the t-DNA in a high-sensitivity manner. The method includes chemically bonding the nanoparticles (SERS labels) comprising SERS codes with p-DNA (phosphorous-deoxyribose nucleic acid) of a probe to prepare a high-sensitivity SERS probe at first; capturing the c-DNA (complementary-deoxyribose nucleic acid) by the magnetic composite nanoparticles with surfaces richly containing carboxyl in a chemical bonding manner to prepare the magnetic capturing substrate; and constructing the SERS liquid chip by the SERS probe and the magnetic capturing substrate to detect the t-DNA. The method is simple, speedy and sensitive in operation, can be used for high through-put, quantitative and multi-element detection for DNA (deoxyribose nucleic acid), can be widely used in fields such as food safety monitoring, medical diagnosis and forensic examination, and has an important application prospect and a development value.
Description
Technical field
The invention belongs to the biomolecule detection technical field, be specifically related to the SERS liquid-phase chip method of a kind of highly sensitive detection t-DNA.
Background technology
The detection of dna molecular has important use and is worth in fields such as gene therapy, food safety monitoring, medical diagnosis, forensic analysis, verification retrievals.At present, using maximum detection methods is fluorescent spectrometry.But fluorescence is easy to the reappearance extreme difference that cancellation causes fluorescence signal.Fluorescence emission spectrum broad (half high spectrum width of organic fluorescent dye is 50～100 nm, and half high spectrum width of quantum dot is 25～40 nm) causes emission spectrum easy overlapping in addition, thereby the resolution of fluorescence spectrum very low (Sun, L.; Yu, C. X.; Irudayaraj, J. Anal. Chem. 2007,79,3981-3988).Compare with fluorescence spectrum; SERS spectrum (SERS) has following advantage aspect DNA detection: one, SERS spectrum can provide abundant molecular vibration information; SERS spectrum spectrum peak narrow (about 1 nm of halfwidth), thereby SERS spectrum has very high spectral resolution.Two, the raman active molecule light stability is high, the favorable reproducibility of SERS signal (Mulvaney, S. P.; Musick, M. D.; Keating, C. D.; Natan, M. J. Langmuir 2003,19,4784-4790).Therefore, SERS spectrum has replaced fluorescence spectrum gradually at present, becomes the detection method of bioanalysis field widespread usage.
The basic skills of using SERS spectral detection DNA is " sandwich " hydridization analytic approach, and anchoring base commonly used at present is a sheet film chip.Mirkin seminar at first has sulfydryl and an end with an end and has combined the p-DNA of raman active molecule to be adsorbed onto the golden nanometer particle surface to process the SERS probe; And planar chip covalent bond c-DNA processed catch substrate, adopt " sandwich " hydridization analytical approach to detect t-DNA then.Experimental result obtains detecting and is limited to 20 fM, and this method has higher selectivity and spectral resolution, and can detect dna sequence dna (Cao, the Y. W. C. of single base mispairing; Jin, R. C.; Mirkin, C. A. Science 2002,297,1536-1540).Braun etc. combine c-DNA as catching substrate smooth silver-colored nanometer film, and after forming the hydridization compound, Nano silver grain in the SERS probe and silver-colored nanometer film substrate closely are close together and produce huge SERS signal (Braun, G.; Lee, S. J.; Dante, M.; Nguyen, T. Q.; Moskovits, M.; Reich, N.J. Am. Chem. Soc. 2007,129,6378-6379).The preparation method is simple for sheet film chip anchoring base, but has following shortcoming: the hybridization reaction between (1) DNA chain carries out in solid phase, greatly reduces the hybridization efficiency between the DNA chain; (2) specific surface area of sheet film chip is less, big limitations the binding capacity of c-DNA; (3) because c-DNA is combined in the entire chip surface, thereby the SERS probe is evenly distributed in whole substrate surface (be far longer than SERS detect laser facula area), thereby the signal intensity of having disperseed the SERS probe has reduced detection sensitivity.Therefore, easy, efficient, the highly sensitive and high-throughout DNA detection method of exploitation is still the focus of research in recent years.
Summary of the invention
The object of the present invention is to provide the SERS liquid-phase chip method of a kind of easy, efficient, highly sensitive detection t-DNA.
The present invention is directed to existing problem in the background technology; Having proposed the SERS coding microball that a kind of employing has high sensitivity and high stability is the SERS probe, and the SERS liquid-phase chip that has high-specific surface area and the magnetic composite nanoparticles of magnetic responsiveness fast and be anchoring base detects the method for t-DNA.Magnetic composite nanoparticles has fast magnetic responsiveness ability, excellent biological compatibility ability and higher DNA and catches capacity and efficient; SERS liquid-phase chip method based on SERS probe and magnetic composite nanoparticles structure has high detection sensitivity, high detection by quantitative ability and high multivariate detection ability.
The present invention realizes through following three step technical schemes; The first step prepares the SERS probe: at first use amino silicane coupling agent to be functional compounds; Adopting sol-gel method is amino group with the surface modification of the SERS label of coated silica shell; Utilize the ring-opening reaction of amino and succinic anhydride then, obtain the SERS label of finishing carboxyl, utilize the carboxyl of SERS label surface and the terminal amino generation amidation process of p-DNA to obtain the SERS probe at last.Second step preparation magnetic catch substrate: make the carboxyl on magnetic composite nanoparticles surface and the terminal amino generation amidation process of c-DNA obtain the magnetic catch substrate through the carbodiimide method.The 3rd step made up SERS liquid-phase chip method: use the SERS probe of t-DNA and coupling and magnetic composite nanoparticles structure SERS liquid-phase chip in solution, to adopt " sandwich " hydridization analytical approach to detect t-DNA (as shown in Figure 1).
The SERS liquid-phase chip method of a kind of highly sensitive detection t-DNA provided by the invention, concrete steps are following:
1. prepare the SERS probe
With shell is that the high stability of silicon dioxide and the SERS label microballoon of biocompatibility are dispersed in the mixed solution of water and ethanol; Add ammoniacal liquor and a certain amount of amino silicane coupling agent again; Ultrasonic emulsification 0.2～2h; Ultrasonic power is 400～800W, obtains the amino SERS label microballoon of finishing.Then that above-mentioned finishing is amino SERS label; Succinic anhydride and solvent N, N '-dimethyl formamide (DMF) adds the single port flask, behind 5～20 h of magnetic agitation reaction at room temperature; Water and ethanol centrifuge washing are removed excessive reagent, obtain the SERS label of finishing carboxyl.At last with the SERS label and the amidation process catalyzer of finishing carboxyl; The phosphate buffer solution of p-DNA (PBS) mixes; Hatch 2～20 h in the shaking table at room temperature; The SERS probe that obtains is removed excessive reagent with the PBS centrifuge washing, is dispersed in the PBS solution of sodium chloride-containing again and is made into the dispersion liquid that particle content is 0.5 wt%.
2. preparation magnetic catch substrate
Magnetic composite nanoparticles is mixed with the phosphate buffer solution (PBS) of amidation process catalyzer, c-DNA; Hatch 2～20 h in the shaking table at room temperature; The magnetic catch substrate that obtains is removed excessive reagent with PBS magnetic separating, washing, is dispersed in then and is made into the dispersion liquid that particle content is 0.5 wt% in the PBS solution that contains sodium chloride.
3.SERS liquid-phase chip detects
The SERS probe is mixed with the t-DNA that is dissolved in the PBS solution that contains NaCl; In 10～60 ℃ shaking table, hatch 2～10 h; Add the magnetic catch substrate then and in 10～60 ℃ shaking table, continue hatching 2～10 h; Obtain " sandwich " formula hydridization compound, remove the SERS probe of physisorption again with PBS magnetic separating, washing, measure with carrying out Raman after the externally-applied magnetic field enrichment at last.
Amino silicane coupling agent described in the present invention is a kind of in 3-aminopropyl triethoxysilane (APTES) and the 3-aminopropyl trimethoxysilane (APTMS).
Among the present invention, the consumption of the amino silicane coupling agent described in the step 1 is 0.1～10g/g SERS label microballoon.
Among the present invention, the amidation process catalyzer described in step 1 and the step 2 is one or both in 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) solution, the N-hydroxy-succinamide (NHS).
Among the present invention, the succinic anhydride consumption described in the step 1 is 0.1～10g/g SERS label microballoon, and the amidation process catalyst amount is 1～100g/g SERS label microballoon, and probe p-DNA concentration is 0.1～100 μ M.
Among the present invention, the amidation process catalyst amount described in the step 2 is 1～100g/g magnetic composite nanoparticles, and catching c-DNA concentration is 0.1～100 μ M.
Among the present invention, the preparation method of the SERS label described in the step 1 adopts patent (publication number CN102206357A) reported method.
Among the present invention, the magnetic composite nanoparticles described in the step 2 is a kind of surface carboxyl functionalized core-shell type magnetic composite microsphere, and patent (application number 201110274619.8) reported method is adopted in its preparation.
Among the present invention, the SERS label microballoon of silicon dioxide is to be the SERS label of Raman microprobe molecule with 4-aminothiophenol, 4-chlorothio-phenol or 5,5 ＇-two thiobis (2-nitrobenzoic acid) in the step 1.
The SERS liquid-phase chip method that the present invention adopts the magnetic composite nanoparticles of SERS probe and the Nano grade of SERS coding to make up has overcome the shortcoming of problems such as hybridization efficiency combination quantity low, that catch c-DNA is few between the planar chip method DNA chain, detection sensitivity is low, and this SERS liquid-phase chip is used to detect DNA and has the following advantages:
(1) the magnetic composite nanoparticles substrate has bigger specific surface area than planar substrates, can directly in solution, dynamically catch biomolecule, and hydridization efficient and capture rate between the DNA chain improve greatly, therefore has bigger detection sensitivity;
(2) can use externally-applied magnetic field to separate the hydridization compound fast, reduce the restriction of mass transfer in the detachment process;
(3) use the external magnetic field can the hydridization compound be assembled, thereby the amplification detection signal further improve detection sensitivity; (4) this method of operating is simple, quick, efficient.
Description of drawings
Fig. 1 is a detection principle schematic of the present invention.
Fig. 2 is the SERS spectrum of the SERS probe in detecting t-DNA of embodiment 1 embedding 4-aminothiophenol.
Embodiment
Embodiment 1:
Capture dna (c-DNA): 5 ' NH
2(A
10) TCTATAAACCTTATT (SEQ.ID. NO.1)
Target dna (t-DNA): AGATATTTGGAATAACATGACCTGGATGCA (SEQ.ID. NO.2)
Dna probe (p-DNA): GTACTGGACCTACGT (A
10) NH
23 ' (SEQ.ID. NO.3)
1, preparation SERS probe
Three steps below the preparation of SERS probe divides accomplish:
The first step is modified as amino with the SERS label surface:
SERS label 10 mg of the embedding 4-aminothiophenol (4-ABT) of patent (CN102206357A) report are dispersed in the mixed solution of 9 g water and 32 g ethanol; Add 1 g ammoniacal liquor and 0.1 g 3-aminopropyl triethoxysilane (APTES) again; Ultrasonic emulsification 1 h; Ultrasonic power is 600 W, obtains the amino SERS label microballoon of finishing.
Second step was a carboxyl with the amino modified of SERS label surface:
The SERS label that 20 mg finishinges are amino; 20 mg succinic anhydrides, 20 mL DMF add 50 mL single port flasks, at room temperature behind magnetic agitation 10 h; Product water and ethanol centrifuge washing is respectively removed excessive reagent three times, obtains the SERS label of finishing carboxyl.
The 3rd step adopted the carbodiimide method to obtain the SERS probe at SERS label surface chemical bond 3 ' terminal probe p-DNA for amino:
With the SERS label and the 2 mL EDC solution (10 mg/ml) of 1 mg finishing carboxyl, after 1 mL, 1 μ M p-DNA phosphate buffer solution (PBS) mixes, hatching 12 h in the shaking table at room temperature.The product S ERS probe that obtains is removed excessive reagent three times with the PBS centrifuge washing.At last above-mentioned product is dispersed in the PBS solution of 0.6 M NaCl and is made into the dispersion liquid that particle content is 0.5 wt%.
2, the preparation of magnetic catch substrate
Adopt the carbodiimide method is rich in carboxyl on the surface magnetic composite nanoparticles surface chemistry to combine 5 ' the terminal c-DNA that catches to prepare the magnetic catch substrate for amino:
With the magnetic composite nanoparticles and the 2 mL EDC solution (10 mg/ml) of 1 mg patent (application number 201110274619.8) report, after 1 mL, 1 μ M c-DNA phosphate buffer solution (PBS) mixes, hatching 12 h in the shaking table at room temperature.The product magnetic catch substrate that obtains is removed excessive reagent three times with PBS magnetic separating, washing, is dispersed in then in the PBS solution of 0.6 M NaCl and is made into the dispersion liquid that particle content is 0.5 wt%.
3, use the process that SERS liquid-phase chip method detects DNA
Adopt SERS liquid-phase chip method to detect target t-DNA:
With 100 μ L SERS probes with after the t-DNA 0.6 M NaCl PBS solution of 10 μ L variable concentrations mixes; Hatching 4 h in 25 ℃ shaking table; Add 100 μ L then and combined the magnetic composite nanoparticles of c-DNA in 25 ℃ shaking table, to continue hatching 4 h, " sandwich " formula hydridization compound that obtains is removed the SERS probe of physisorption for three times with PBS magnetic separating, washing.At last the hydridization compound is changed in the glass groove, measure with carrying out Raman after the externally-applied magnetic field enrichment.When the concentration of t-DNA is 10
-8～10
-12During M, a series of SERS spectrogram that obtains is shown in accompanying drawing 2.Obtain detecting and be limited to 10
-12M.
Embodiment 2:
Capture dna (c-DNA): 5 ' NH
2(A
10) AACCGAAAGTCAATA (SEQ.ID. NO.4)
Target dna (t-DNA): TTGGCTTTCAGTTATATGGATGATGTGGTA (SEQ.ID. NO.5)
Dna probe (p-DNA): TACCTACTACACCAT (A
10) NH
23 ' (SEQ.ID. NO.6)
1, the preparation of SERS probe is with described in the embodiment 1-1.The raman labels molecule of SERS label embedding that different is is 4-chlorothio-phenol (4-CBT).
2, the preparation of magnetic catch substrate is with described in the embodiment 1-2.
3, the process of using SERS liquid-phase chip method detection t-DNA is with described in the embodiment 1-3.
Obtain detecting and be limited to 10
-12M.
Embodiment 3:
Capture dna (c-DNA): 5 ' NH
2(A
10) AATCTCAACGTACCT (SEQ.ID. NO.7)
Target dna (t-DNA): TTAGAGTTGCATGGATTA ACTCCTCTTTCT (SEQ.ID. NO.8)
Dna probe (p-DNA): AATTGAGGAGAAAGA (A
10) NH
23 ' (SEQ.ID. NO.9)
1, the preparation of SERS probe is with described in the embodiment 1-1.The raman labels molecule of SERS label embedding that different is be 5,5 ＇-two thiobis (2-nitrobenzoic acid) (DTNB).
2, the preparation of magnetic catch substrate is with described in the embodiment 1-2.
3, the process of using SERS liquid-phase chip method detection DNA is with described in the embodiment 1-3.
Obtain detecting and be limited to 10
-11M.
Sequence table
SEQ.ID.?NO.1：?TCTATAAACCTTATT
SEQ.ID.?NO.2：?AGATATTTGGAATAACATGACCTGGATGCA
SEQ.ID.?NO.3：?GTACTGGACCTACGT
SEQ.ID.?NO.4：?AACCGAAAGTCAATA
SEQ.ID.?NO.5：?TTGGCTTTCAGTTATATGGATGATGTGGTA
SEQ.ID.?NO.6：?TACCTACTACACCAT
SEQ.ID.?NO.7：?AATCTCAACGTACCT
SEQ.ID.?NO.8：?TTAGAGTTGCATGGATTAACTCCTCTTTCT
SEQ.ID.?NO.9：?AATTGAGGAGAAAGA
Claims (4)
1. the SERS liquid-phase chip method of a highly sensitive detection t-DNA is characterized in that concrete steps are following:
(1) preparation SERS probe: with shell is that the SERS label microballoon of silicon dioxide is dispersed in the solvent; Add ammoniacal liquor and amino silicane coupling agent; Ultrasonic emulsification obtains the amino SERS label microballoon of finishing; Wherein, said amino silicane coupling agent and shell are that the mass ratio of the SERS label microballoon of silicon dioxide is (0.1～10): 1; Then that above-mentioned finishing is amino SERS label microballoon, succinic anhydride are dissolved in N; N '-dimethyl formamide, stirring at room, centrifugal; Washing; Obtain the SERS label of finishing carboxyl, wherein, the mass ratio of the SERS label microballoon that said succinic anhydride and finishing are amino is (0.1～10): 1; Be that the phosphate buffer solution of the p-DNA of 0.1～100 μ M mixes with the SERS label of finishing carboxyl and amidation process catalyzer, concentration at last; Room temperature hatching 2～20 h; Centrifugal; Washing is dispersed in the phosphate buffer solution of sodium chloride-containing again and is made into dispersion liquid, and the mass ratio of wherein said amidation process catalyzer and SERS label microballoon is (1～100): 1;
(2) preparation magnetic catch substrate: with magnetic composite nanoparticles and amidation process catalyzer, concentration is that the phosphate buffer solution of the c-DNA of 0.1～100 μ M mixes; Room temperature hatching 2～20 h; Separating, washing; Be dispersed in then in the phosphate buffer solution that contains sodium chloride and be made into dispersion liquid, wherein, the mass ratio of said magnetic composite nanoparticles and amidation process catalyzer is 1: (1～100);
(3) the SERS liquid-phase chip detects: the SERS probe of step (1) preparation is mixed with the t-DNA that is dissolved in the phosphate buffer solution that contains NaCl; 10～60 ℃ of hatching 2～10 h; The magnetic catch substrate that adds step (2) preparation then; 10～60 ℃ are continued hatching 2～10 h, and separating, washing is measured with carrying out Raman after the externally-applied magnetic field enrichment again.
2. the SERS liquid-phase chip method of highly sensitive detection t-DNA according to claim 1, it is characterized in that: amino silicane coupling agent described in the step (1) is 3-aminopropyl triethoxysilane or 3-aminopropyl trimethoxysilane.
3. the SERS liquid-phase chip method of highly sensitive detection t-DNA according to claim 1, it is characterized in that: amidation process catalyzer described in step (1) and the step (2) is selected from one or both in 1-ethyl-(3-dimethylaminopropyl) carbodiimide, the N-hydroxy-succinamide.
4. the SERS liquid-phase chip method of highly sensitive detection t-DNA according to claim 1; It is characterized in that: the SERS label microballoon of silicon dioxide is to be the SERS label of Raman microprobe molecule with 4-aminothiophenol, 4-chlorothio-phenol or 5,5 ＇-two thiobis (2-nitrobenzoic acid) in the step (1).
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| CN103127890A (en) * | 2013-03-07 | 2013-06-05 | 复旦大学 | Raman strengthening active microsphere and preparation method and application thereof |
| CN105452850A (en) * | 2013-05-08 | 2016-03-30 | 米特奇有限公司 | Raman quantification method of cancer-related substance |
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| CN103127890A (en) * | 2013-03-07 | 2013-06-05 | 复旦大学 | Raman strengthening active microsphere and preparation method and application thereof |
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| CN108414758B (en) * | 2018-01-12 | 2020-06-19 | 宁波大学 | Preparation method and application of SERS biosensor for detecting tumor marker miRNA-141 |
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