CN102696887B - Feed active egg powder with nutrition, healthcare and growth promotion functions and production method thereof - Google Patents

Feed active egg powder with nutrition, healthcare and growth promotion functions and production method thereof Download PDF

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CN102696887B
CN102696887B CN 201210226618 CN201210226618A CN102696887B CN 102696887 B CN102696887 B CN 102696887B CN 201210226618 CN201210226618 CN 201210226618 CN 201210226618 A CN201210226618 A CN 201210226618A CN 102696887 B CN102696887 B CN 102696887B
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yolk antibody
powder
egg
yolk
feed
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CN102696887A (en
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王清平
刘成元
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Safat (Changsha) Biotechnology Co., Ltd.
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HUNAN HAIGU BIOLOGICAL TECHNOLOGY CO LTD
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Abstract

The invention discloses feed active egg powder with nutrition, healthcare and growth promotion functions and a production method thereof, wherein the feed active egg powder with nutrition, healthcare and growth promotion functions is obtained by fully mixing weighed enterotoxin-resistant Escherichia coli tetravalent yolk antibody, C-type clostridium perfringens and beta toxoid-resistant divalent yolk antibody, rotavirus-resistant yolk antibody, transmissible gastroenteritis and epidemic diarrhea-resistant bigeminal yolk antibody, cholecystokinin/somatostatin-resistant double-fusion white and yolk antibody, lysozyme meringue powder, epidermal growth factor-containing meringue powder, common meringue powder and silica (antitackiness agent) for 60 to 100 seconds. The feed active egg powder with nutrition, healthcare and growth promotion functions provided by the invention has the beneficial effects of containing high-quality protein, fat and amino acid required for the growth and development of piglets, containing lysozyme, yolk antibody and epidermal growth factor for preventing disease, protecting health and promoting growth, and having four efficacies of enhancing the feed intake, accelerating the growth, boosting the immunity and controlling the diarrhea of the piglets before and after weaning.

Description

A kind of active powdered egg of feed and production method thereof with nourishing healthy growth promotion function
Technical field
The present invention relates to animal feed and biomedicine field, be specially a kind of active powdered egg of feed and production method thereof with nourishing healthy growth promotion function.
Background technology
The sucking pig early weaning not only can be brought into play sow production capacity to greatest extent, improves and produces parity per year, and can reduce transmission of disease between female son.Yet, behind the early weaning piglet, can be subjected to various unfavorable factors stress with the invasion and attack of cause of disease, it is grown and survive has produced bigger influence, situation such as occur searching for food that declines, diarrhoea are lost flesh, hair color dries up, growth retardation, survival rate are low is commonly called as " weaning syndrome ".Main cause has: 1. behind the weaned piglet, losing its passive immunity protection of breast milk has reduced, and the active immunity system of body does not set up as yet fully, is subjected to the invasion and attack of various cause of diseases such as Escherichia coli, C.perfringens, rotavirus, transmissible gastroenteritis of swine and epidemic diarrhea easily and falls ill; 2. weanling pig has lost somatotrophic some active material of newborn source property, causes the marked change of small intestinal mucosa structure and function, is affected thereby cause body growth to be grown, and disease takes place easily, even dead; 3. feed intake reduces, and digests and assimilates ability drop, subalimentation, growth retardation; 4. the influence of ANFs such as soybean antigen albumen etc. in the feedstuff; 5. ablactation stress can cause the hormonal change of piglet, and some antigrowth hormone secretion amounts increase, from maincenter level affects growth in piglets.
Current, the method that solves a weanling pig difficult problem in the production has: 1. add, use a large amount of antibiotic or chemicals in feed and feeding process, though bacteria resistance diarrhoea is effective like this, but it is invalid to virus diarrhea, and medicament residue is serious, does not meet the food security and the healthy aquaculture development trend of people's growing interest.And use antibiotic can produce drug resistance for a long time, make antibiotic become invalid on the one hand, cause antibiotic abuse on the other hand, the serious harm human health; 2. add 2500PPM zinc oxide in piglet creep feed, the control grice diarrhoea is had certain effect, but influence the hair color of piglet, a large amount of zinc oxide pollute environment.3. use SDPP in the piglet creep feed: SDPP is with slaughterhouse's pig blood, through centrifugation serum and blood cell gained serum, the serum spray-drying is formed again.Contain protein 78%, immunoglobulin (Ig) 10%, and protein digestibility utilization rate height also contain a small amount of somatomedin.Having increases the effect of searching for food, promoting to grow, reduce diarrhoea, generally uses on producing.But, there is bio-safety hidden danger because homology and production procedure do not have process for sterilizing.Owing to differ in pig blood source, the SDPP quality is difficult to stable simultaneously.
Therefore, how to improve the searching for food of weanling pig, digest, absorption and conversion capability, improve the immunity of weanling pig, control diarrhoea property disease takes place, make the weanling pig fast speed cross the child care vulnerable period, reduce or cancel the use of antibiotic medicine, realize the healthy ecology breed, proposed the series of studies problem for feed nutrition and animal medicine circle.
Summary of the invention
Technical problem solved by the invention is to provide a kind of have active powdered egg of the somatotrophic feed of nourishing healthy and production method thereof, to solve the problem in the background technology:
The invention provides the active powdered egg of the somatotrophic feed of a kind of nourishing healthy, the active powdered egg of this feed comprises anti-grice diarrhoea yolk antibody, growth promotion yolk antibody, egg white lysozyme and EGF.Wherein: anti-grice diarrhoea yolk antibody comprises anti-enterotoxin type Escherichia coli tetravalence yolk antibody, anti-C type C.perfringens and β toxoid bivalent antibody, anti-rotavirus yolk antibody, anti-swine infectious enterogastritis/epidemic diarrhea bigeminy yolk antibody, has piglet red dysentery, yellowish-white dysentery, wean back diarrhoea and the transmissible gastroenteritis that control enterotoxin type Escherichia coli, C.perfringens, rotavirus, transmissible gastroenteritis of swine, pig epidemic diarrhea cause, the generation of epidemic diarrhea disease; The growth promotion yolk antibody is the two fusion yolk antibodies of anti-CCK/growth hormone release inhibiting hormone, CCK and growth hormone release inhibiting hormone in the energy and in the body, weaken CCK and growth hormone release inhibiting hormone to search for food, the inhibitory action of gastric acid secretion, digestive ferment and growth hormone, increase the feed intake of piglet, strengthen digestive function, improve the speed of growth; Egg white lysozyme is that a kind of in clear has sterilizing function protein from egg, and is active high, has anti-inflammation, antiviral, the effect that strengthens immunity; EGF can promote the chorial growth of enteron aisle, improves the activity of brush border digestive ferment, improves digesting and assimilating of nutriment, promotes piglet growth.
The invention provides the production method of the active powdered egg of the somatotrophic feed of a kind of nourishing healthy, it comprises the steps:
(1) anti-grice diarrhoea yolk antibody powder preparation:
The immunity of a laying hen: with enterotoxin type Escherichia coli K88 K99 987P F41 tetravalent vaccine, C type C.perfringens and β toxoid bivalent vaccine, Rotavirus Vaccine, transmissible gastroenteritis of swine and epidemic diarrhea bigeminy vaccine, respectively the health reserve chicken of laying eggs is carried out immunity inoculation 3 times, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again at air intake 120-140 ℃, spray-drying, packing under the air draft 55-70 ℃ condition, make anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder, anti-C type C.perfringens and toxin divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder respectively, standby;
(2) preparation of growth promotion yolk antibody powder:
The immunity of a laying hen: with CCK (CCK) and growth hormone release inhibiting hormone (SS) fusion is antigen, adds efficient adjuvant and makes vaccine; The health reserve chicken of laying eggs is carried out immunity inoculation, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again at air intake 120-140 ℃, spray-drying, packing under the air draft 55-70 ℃ condition, make the two fusion yolk antibody powder of anti-CCK and growth hormone release inhibiting hormone, standby;
(3) preparation of lysozyme egg-white powder:
New fresh hen egg through automatic washing, beating eggs obtains egg white after separating, and with citric acid the pH value of egg white solution is transferred to 4-7, again at air intake 120--130 ℃, spray-drying, packing are made the lysozyme egg-white powder under the air-out 55-60 ℃ condition, and be standby;
(4 contain the preparation of EGF egg-white powder:
New fresh hen egg obtains egg white after automatic washing, the separation of beating eggs, after with citric acid the pH value of egg white solution being transferred to 6-8, add EGF with dissolved in distilled water, again at air intake 120--130 ℃, spray-drying, packing are made and contained the EGF egg-white powder under the air-out 55-60 ℃ condition, and be standby;
(5) common yolk powder preparation:
Through automatic washing, beating eggs obtains yolk after separating, and refilters, homogeneous, pasteurize, again at air intake 170-180 ℃, spray-drying, packing are made yolk powder under the air-out 70-72 ℃ condition, and be standby;
(6) preparation has the active powdered egg of feed of nourishing healthy growth promotion function
The a preparation is applicable to the active powdered egg of feed that feed factory uses, and teaches in the groove mixed feed in 1% ratio prescription to newborn piglet.
The active powdered egg active ingredient of the described feed of a1 is:
Protein 〉=45%, fat 〉=40%
Anti-enterotoxin type Escherichia coli tetravalence yolk antibody titre 〉=800: 1
Anti-C type C.perfringens and β toxoid divalence yolk antibody titre 〉=800: 1
Anti-rotavirus yolk antibody titre 〉=800: 1
Anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody titre 〉=800: 1
The two fusion yolk antibody titres of anti-CCK/growth hormone release inhibiting hormone 〉=480: 1
Egg white lysozyme 〉=1.5*108IU/kg
EGF 〉=10mg/kg
Active powdered egg component of the described feed of a2 and percentage by weight:
Figure BSA00000743624600041
A3 weighing and mixing: take by weighing anti-enterotoxin type Escherichia coli tetravalence yolk antibody respectively by above-mentioned percentage by weight, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, the two fusion yolk antibodies of anti-CCK/growth hormone release inhibiting hormone, the lysozyme egg-white powder, contain the EGF egg-white powder, common yolk powder, silica (anticaking agent), mix 60--100 second, obtain the active powdered egg of the feed with nourishing healthy growth promotion function that is applicable to that feed factory uses.
The b preparation is applicable to the active powdered egg of the feed that uses on the pig farm, is added into the newborn compound feed for piglets in wean front and back in 0.2% ratio.
The active powdered egg active ingredient of the described feed of b1 is:
Protein 〉=30%, fat 〉=60%
Anti-enterotoxin type Escherichia coli tetravalence yolk antibody titre 〉=3200: 1
Anti-C type C.perfringens and β toxoid divalence yolk antibody titre 〉=3200: 1
Anti-rotavirus yolk antibody titre 〉=3200: 1
Anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody titre 〉=3200: 1
The two fusion yolk antibody titres of anti-CCK/growth hormone release inhibiting hormone 〉=2400: 1
EGF 〉=50mg/kg
Active powdered egg component of the described feed of b2 and percentage by weight:
Figure BSA00000743624600051
B3 weighing and mixing: take by weighing anti-enterotoxin type Escherichia coli tetravalence yolk antibody, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, the two fusion yolk antibodies of anti-CCK/growth hormone release inhibiting hormone respectively, contain EGF egg-white powder, silica (anticaking agent) by above-mentioned percentage by weight, mix 60--100 second, obtain being applicable to the active powdered egg of the feed that uses on the pig farm with nourishing healthy growth promotion function.
Beneficial effect:
The invention has the beneficial effects as follows provides a kind of active powdered egg of feed with nourishing healthy growth promotion function, not only contain newborn piglet growth and grow required good protein, fat, amino acid, and also have the somatotrophic lysozyme of disease-prevention health, yolk antibody and EGF, have the feed intake of raising, accelerate the speed of growth, strengthen immunity, diarrhoea four large effects before and after the newborn weaned piglet of control.The active powdered egg of feed can replace fully current in piglet creep feed widely used SDPP, show through the feeding comparative trial, the active powdered egg of feed is to feed intake, daily gain, feed conversion rate and anti-diarrhea to improve effect better than SDPP, and has overcome the problem of SDPP biological safety.The interpolation of using the active powdered egg of feed can reduce or fully phase out health care growth promotion medicine in feed and feeding process uses and heavy dose of zinc oxide uses in piglet feed, guarantees healthy aquaculture, the food security of people's growing interest.
The specific embodiment
Embodiment one, a kind of active powdered egg of feed of the present invention with nourishing healthy growth promotion function, and commodity are called " U egg treasured ", are suitable for adding in the newborn compound feed for piglets, and adding proportion is 1.0%,
According to producing the active powdered egg U of the feed egg treasured that 100kg has health care growth promotion function, its preparation method comprises the steps:
(1) anti-grice diarrhoea yolk antibody powder preparation
The immunity of a laying hen: with enterotoxin type Escherichia coli K88 K99 987P F41 tetravalent vaccine, C type C.perfringens and β toxoid bivalent vaccine, Rotavirus Vaccine, transmissible gastroenteritis of swine and epidemic diarrhea bigeminy vaccine, respectively the health reserve chicken of laying eggs is carried out immunity inoculation 3 times, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again 130 ℃ of air intakes, spray-drying, packing under 65 ℃ of conditions of air draft are made anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder respectively.Wherein: antibody titer all 〉=16000: 1, protein all 〉=30%, fat all 〉=60%.
(2) preparation of growth promotion yolk antibody powder:
The immunity of a laying hen: with CCK (CCK) and growth hormone release inhibiting hormone (SS) fusion is antigen, adds efficient adjuvant and makes vaccine; The health reserve chicken of laying eggs is carried out immunity inoculation, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again 130 ℃ of air intakes, spray-drying, packing under 65 ℃ of conditions of air draft are made the two fusion yolk antibody powder of anti-CCK and growth hormone release inhibiting hormone.Wherein: yolk antibody powder antibody titer all 〉=16000: 1, protein all 〉=30%, fat all 〉=60%.
(3) preparation of lysozyme egg-white powder:
New fresh hen egg through automatic washing, beating eggs obtains egg white after separating, and with citric acid the pH value of egg white solution is transferred to 6.0, again 125 ℃ of air intakes, spray-drying, packing are made the lysozyme egg-white powder under 58 ℃ of conditions of air-out.Wherein: lysozyme 〉=5.0*10 8IU/kg, protein 〉=80%
(4) contain the preparation of EGF egg-white powder:
New fresh hen egg obtains egg white after automatic washing, the separation of beating eggs, after with citric acid the pH value of egg white solution being transferred to 8.0, add the EGF with dissolved in distilled water, again 125 ℃ of air intakes, spray-drying, packing are made and are contained the EGF egg-white powder under 58 ℃ of conditions of air-out.Wherein: EGF 〉=1000mg/kg, protein 〉=80%
(5) common yolk powder preparation:
Through automatic washing, beating eggs obtains yolk after separating, and refilters, homogeneous, pasteurize, again 170 ℃ of air intakes, spray-drying, packing are made yolk powder under 70 ℃ of conditions of air-out.Wherein: protein all 〉=30%, fat all 〉=60%.
(6) preparation has the active powdered egg of feed of nourishing healthy growth promotion function
A weighing: take by weighing the two fusion yolk antibody 3kg of anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder 5kg, anti-C type C.perfringens and β toxoid divalence yolk antibody powder 5kg, anti-rotavirus yolk antibody powder 5kg, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder 5kg, anti-CCK and growth hormone release inhibiting hormone respectively, contain EGF egg-white powder 1kg, lysozyme egg-white powder 30.5kg, common yolk powder 44kg, silica 1 .5kg.
B mixes: with the two fusion yolk antibody powder of the anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder that is taken by weighing, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, anti-CCK and growth hormone release inhibiting hormone, contain EGF egg-white powder, lysozyme egg-white powder, common yolk powder, silica, fully mix after 90 seconds, obtain having the active powdered egg of feed of nourishing healthy growth promotion function.
Embodiment two, a kind of active powdered egg of feed of the present invention with health care growth promotion function, and commodity " sky adds health " by name are suitable for adding in the Pre-and Post-Weaning Piglets mixed feed, and adding proportion is 0.2%
The active powdered egg of the feed sky that has health care growth promotion function according to production 100kg adds health, and its preparation method comprises the steps:
(1) anti-grice diarrhoea yolk antibody powder preparation
The immunity of a laying hen: with enterotoxin type Escherichia coli K88 K99 987P F41 tetravalent vaccine, C type C.perfringens and β toxoid bivalent vaccine, Rotavirus Vaccine, transmissible gastroenteritis of swine and epidemic diarrhea bigeminy vaccine, respectively the health reserve chicken of laying eggs is carried out immunity inoculation 3 times, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again 130 ℃ of air intakes, spray-drying, packing under 65 ℃ of conditions of air draft are made anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder respectively.Wherein: antibody titer all 〉=16000: 1, protein all 〉=30%, fat all 〉=60%.
(2) preparation of growth promotion yolk antibody powder
The immunity of a laying hen: with CCK (CCK) and growth hormone release inhibiting hormone (SS) fusion is antigen, adds efficient adjuvant and makes vaccine; The health reserve chicken of laying eggs is carried out immunity inoculation, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, again 130 ℃ of air intakes, spray-drying, packing under 65 ℃ of conditions of air draft are made the two fusion yolk antibody powder of anti-CCK and growth hormone release inhibiting hormone.Wherein: yolk antibody powder antibody titer all 〉=16000: 1, protein all 〉=30%, fat all 〉=60%.
(3) contain the preparation of EGF egg-white powder
New fresh hen egg obtains egg white after automatic washing, the separation of beating eggs, after with citric acid the pH value of egg white solution being transferred to 8.0, add the EGF with dissolved in distilled water, again 125 ℃ of air intakes, spray-drying, packing are made and are contained the EGF egg-white powder under 58 ℃ of conditions of air-out.Wherein: EGF 〉=5000mg/kg, protein 〉=80%
(4) preparation has the active powdered egg of feed of nourishing healthy growth promotion function
A weighing: take by weighing the two fusion yolk antibody 15kg of anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder 22.5kg, anti-C type C.perfringens and β toxoid divalence yolk antibody powder 20kg, anti-rotavirus yolk antibody powder 20kg, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder 20kg, anti-CCK and growth hormone release inhibiting hormone respectively, contain EGF egg-white powder 1kg, silica 1 .5kg.
B mixes: with the two fusion yolk antibody powder of the anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder that is taken by weighing, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, anti-CCK and growth hormone release inhibiting hormone, contain EGF egg-white powder, silica, fully mix after 90 seconds, obtain having the active powdered egg of feed of nourishing healthy growth promotion function.
Embodiment three, the effect test on porket creep of the active powdered egg U of feed egg treasured
1, test method: 96 of the near health 21 age in days weanling pigs of selective body heavy phase, be randomized into test group and control group in proportion by male and female, every component is 4 hurdles, 12 on every hurdle, 14 days experimental periods.
Control group: 4% SDPP+basestocks, add medicine: kitasamycin 40ppm, colistine sulfate 20ppm, zinc oxide 2500ppm;
Test group: 1%U egg treasured+2% soybean protein isolate+1% wheat gluten+basestocks does not add any medicine and zinc oxide
2, result of the test: active powdered egg test group and SDPP control group compare, and daily gain improves 14.6%, and feed intake improves 10.9%, and feed reduces by 3.3%, and cost per gain weight descends 4.6%, and diarrhea rate has reduced by 80%; Active powdered egg test group is not used any antibiotic medicine and zinc oxide, and hair color is ruddy, guaranteeing to have accomplished no resistance, noresidue, nuisanceless under the prerequisite that the sucking pig production performance improves, has realized the green health breed.
Precious and the SDPP effect contrast test on porket creep of the active powdered egg U of feed egg
Figure BSA00000743624600091
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.

Claims (1)

1. the active powdered egg of feed with nourishing healthy growth promotion function is characterized in that its production method comprises the steps:
(1) anti-grice diarrhoea yolk antibody powder preparation:
The immunity of a laying hen: with enterotoxin type Escherichia coli K88 K99 987P F41 tetravalent vaccine, C type C.perfringens and β toxoid bivalent vaccine, Rotavirus Vaccine, transmissible gastroenteritis of swine and epidemic diarrhea bigeminy vaccine, respectively the health reserve chicken of laying eggs is carried out immunity inoculation 3 times, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, spray-drying, packing under air intake 120-140 ℃, air draft 55-70 ℃ condition again, make anti-enterotoxin type Escherichia coli tetravalence yolk antibody powder, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder respectively, standby;
(2) preparation of growth promotion yolk antibody powder:
The immunity of a laying hen: with CCK (CCK) and growth hormone release inhibiting hormone (SS) fusion is antigen, adds efficient adjuvant and makes vaccine; The health reserve chicken of laying eggs is carried out immunity inoculation, detect yolk antibody titre 〉=after 16000: 1, begin to collect immunity eggs through ELISA;
The preparation of b yolk antibody powder: immunity eggs is after ELISA detection yolk antibody titre is qualified, after automatic washing, the separation of beating eggs, obtain yolk, refilter, homogeneous, pasteurize, spray-drying, packing under air intake 120-140 ℃, air draft 55-70 ℃ condition again, make the two fusion yolk antibody powder of anti-CCK and growth hormone release inhibiting hormone, standby;
(3) preparation of lysozyme egg-white powder:
New fresh hen egg through automatic washing, beating eggs obtains egg white after separating, and with citric acid the pH value of egg white solution is transferred to 4-7, again under air intake 120-130 ℃, air-out 55-60 ℃ condition spray-drying, pack and make the lysozyme egg-white powder, standby;
(4) contain the preparation of EGF egg-white powder:
New fresh hen egg obtains egg white after automatic washing, the separation of beating eggs, after with citric acid the pH value of egg white solution being transferred to 6-8, add EGF with dissolved in distilled water, spray-drying, packing are made and contained the EGF egg-white powder under air intake 120-130 ℃, air-out 55-60 ℃ condition again, and be standby;
(5) common yolk powder preparation:
Through automatic washing, beating eggs obtains yolk after separating, and refilters, homogeneous, pasteurize, spray-drying, packing under air intake 170-180 ℃, air-out 70-72 ℃ condition are made yolk powder again, and be standby;
(6) preparation has the active powdered egg of feed of nourishing healthy growth promotion function
The a preparation is applicable to the active powdered egg of feed that feed factory uses, and teaches in the groove mixed feed in 1% ratio prescription to newborn piglet;
The active powdered egg active ingredient of the described feed of a1 is:
Crude protein 〉=45%, fat 〉=40%
Anti-enterotoxin type Escherichia coli tetravalence yolk antibody titre 〉=800: 1
Anti-C type C.perfringens and β toxoid divalence yolk antibody titre 〉=800: 1
Anti-rotavirus yolk antibody titre 〉=800: 1
Anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody titre 〉=800: 1
The two fusion yolk antibody titres of anti-CCK/growth hormone release inhibiting hormone 〉=480: 1
Egg white lysozyme 〉=1.5 * 10 8IU/kg
EGF 〉=10mg/kg
Active powdered egg component of the described feed of a2 and percentage by weight:
Figure FSB00001045227900021
Figure FSB00001045227900031
A3 weighing and mixing: take by weighing anti-enterotoxin type Escherichia coli tetravalence yolk antibody respectively by above-mentioned percentage by weight, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, the two fusion yolk antibodies of anti-CCK/growth hormone release inhibiting hormone, the lysozyme egg-white powder, contain the EGF egg-white powder, common yolk powder, silica, mix 60-100 second, obtain the active powdered egg of the feed with nourishing healthy growth promotion function that is applicable to that feed factory uses;
The b preparation is applicable to the active powdered egg of the feed that uses on the pig farm, is added into the newborn compound feed for piglets in wean front and back in 0.2% ratio;
The active powdered egg active ingredient of the described feed of b1 is:
Crude protein 〉=30%, fat 〉=60%
Anti-enterotoxin type Escherichia coli tetravalence yolk antibody titre 〉=3200: 1
Anti-C type C.perfringens and β toxoid divalence yolk antibody titre 〉=3200: 1
Anti-rotavirus yolk antibody titre 〉=3200: 1
Anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody titre 〉=3200: 1
The two fusion yolk antibody titres of anti-CCK/growth hormone release inhibiting hormone 〉=2400: 1
EGF 〉=50mg/kg
Active powdered egg component of the described feed of b2 and percentage by weight:
Figure FSB00001045227900041
B3 weighing and mixing: take by weighing anti-enterotoxin type Escherichia coli tetravalence yolk antibody, anti-C type C.perfringens and β toxoid divalence yolk antibody powder, anti-rotavirus yolk antibody powder, anti-swine infectious enterogastritis and epidemic diarrhea bigeminy yolk antibody powder, the two fusion yolk antibodies of anti-CCK/growth hormone release inhibiting hormone respectively, contain EGF egg-white powder, silica by above-mentioned percentage by weight, mix 60-100 second, obtain being applicable to the active powdered egg of the feed that uses on the pig farm with nourishing healthy growth promotion function.
CN 201210226618 2012-06-20 2012-06-20 Feed active egg powder with nutrition, healthcare and growth promotion functions and production method thereof Active CN102696887B (en)

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CN103355498B (en) * 2013-07-26 2014-10-22 河南碧云天饲料有限公司 Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs
CN104543580B (en) * 2015-01-22 2016-04-20 四川傲农生物科技有限公司 The piglet diet promoting the premix that weanling pig searches for food and be made up of this premix
CN104970224A (en) * 2015-06-12 2015-10-14 青岛大信饲料有限公司 Composition and mixed feed for preventing and treating porcine epizootic diarrhea
CN107950788A (en) * 2017-11-23 2018-04-24 安徽金牧饲料有限公司 Biological feedstuff composition and its application containing organic trace element

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