CN102696483A - Method for quickly propagating lilium fargesii - Google Patents
Method for quickly propagating lilium fargesii Download PDFInfo
- Publication number
- CN102696483A CN102696483A CN2012102032277A CN201210203227A CN102696483A CN 102696483 A CN102696483 A CN 102696483A CN 2012102032277 A CN2012102032277 A CN 2012102032277A CN 201210203227 A CN201210203227 A CN 201210203227A CN 102696483 A CN102696483 A CN 102696483A
- Authority
- CN
- China
- Prior art keywords
- medium
- callus
- green colored
- illumination
- bud
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a method for quickly propagating lilium fargesii. The method comprises the following steps: I, selecting a disease-free scale leaf on the underground bulb of the lilium fargesii, and cleaning, rinsing and sterilizing the scale leaf to obtain an explant; II, inoculating the explant on a callus induction culture medium to generate a callus after two months; III, inoculating the callus on a subculture culture medium, and executing proliferation culture to generate a new enlarged callus; IV, inoculating the enlarged callus or the new callus on a bud differentiation culture medium to execute bud differentiation derivation, and differentiating multiple shoots; V, inoculating the differentiated multiple shoots on a rooting medium to obtain a rhizogenic lilium fargesii seedling; and VI, inoculating and cultivating the rhizognic lilium fargesii seedling on a cultivation culture medium to obtain a lilium fargesii plant regeneration. The method disclosed by the invention is not restricted by seasons, can perennially achieve quick propagation and large-scale production of the lilum fargesii, and provides an operable method for quickly enlarging the population thereof.
Description
Technical field
The invention belongs to technical field of plant culture, be specifically related to a kind of green colored lily method for quickly breeding.
Background technology
The Qinling Mountains is as the line of demarcation in China north and south, and is with a varied topography, and weather is various, and the wild lily that has been found that just has 11 kinds, and resource is very abundant, and has exploitation value.Lily is world-renowned cut-flower and potted flower, and pattern flower type is different, often has graceful fragrance, how pharmaceutically acceptable with view and admire, economic worth is very high.Green colored lily is wherein a kind of, is described as " spending middle giant panda ", and is endangered now, for better protection with utilize precious green colored lily resource, at first must breed in a large number, enlarge its population fast.
Summary of the invention
Technical problem to be solved by this invention is the deficiency to above-mentioned prior art, and a kind of season limit that do not receive is provided, and can breed green colored lily throughout the year, fast, realizes the green colored lily method for quickly breeding of the large-scale production of green colored lily.Adopt this method to breed green colored lily, rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of green colored lily method for quickly breeding is characterized in that this method may further comprise the steps:
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 0.5h～1.5h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.5mg/L～2.5mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said callus inducing medium is 5.4～5.8; Said culture condition is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 12～18 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L～2.0mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said subculture medium is 5.4～5.8; The condition of said enrichment culture is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 40～50 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L～1.5mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said bud differential medium is 5.4～5.8; The condition of culture of said bud induction is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 10～15 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L～1.2mg/L, methyl 0.02mg/L～0.08mg/L and agar 6g/L～12g/L; The pH value of said root media is 5.4～5.8; Said culture condition is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 5～7: 1～3: 2.
Above-mentioned green colored lily method for quickly breeding, callus inducing medium described in the step 2 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said callus inducing medium is 5.6.
Above-mentioned green colored lily method for quickly breeding, subculture medium described in the step 3 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.2mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said subculture medium is 5.6.
Above-mentioned green colored lily method for quickly breeding, the differential medium of bud described in the step 4 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said bud differential medium is 5.6.
Above-mentioned green colored lily method for quickly breeding, root media described in the step 5 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L, methyl 0.05mg/L and agar 9g/L; The pH value of said root media is 5.6.
Above-mentioned green colored lily method for quickly breeding, the peat composed of rotten mosses described in the step 6, perlite and husky volume ratio are 6: 2: 2.
The present invention compared with prior art has the following advantages:
1, callus inducing medium of the present invention is with strong points, applicability good, and explant scale after callus inducing medium is cultivated 4 days begins to turn green, and the scale edge has the milky callus to produce after 8 days; Continue to cultivate and observe, whole scale has callus to produce after 20 days, and the callus growing way is best; Color is the denseest; Be bottle green, be the graininess of densification, produce a large amount of callus after 2 months.
2, subculture medium cultivation effect of the present invention is good; Callus growth is good; Cultivate and begin to have the callus increase after 12～18 days and have new callus to produce; Newborn callus increases about three times than original callus, and the callus color of generation is a strong green, and no browning occurs; Callus begins differentiation and sprouts after the bud differential medium is cultivated 40～50 days, the bud quantity of generation is more, and germination rate reaches as high as 93.3%, and bud is bottle green, and is healthier; Bud after the differentiation produces root after root media is cultivated 15 days, rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%.
3, method of the present invention does not receive season limit, can breed green colored lily throughout the year, fast, realizes the large-scale production of green colored lily, for enlarging its population fast practicable method is provided.
Through embodiment, technical scheme of the present invention is done further detailed description below.
Embodiment
Embodiment 1
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 1h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant; Said conventional explant material sterilizing methods is: scaly leaf is used quality purity on the desinfection chamber workbench be 75% ethanol disinfection 30s, throws in 5min～10min in 0.1% the mercuric chloride thimerosal then, uses aseptic water washing at last 2～3 times;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce a large amount of callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said callus inducing medium is 5.6; Said culture condition is: 23 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1500Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 14 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.2mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said subculture medium is 5.6; The condition of said enrichment culture is: 23 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1500Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 45 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said bud differential medium is 5.6; The condition of culture of said bud induction is: 23 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1500Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 12 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L, methyl 0.05mg/L and agar 9g/L; The pH value of said root media is 5.6; Said culture condition is: 23 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1500Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 6: 2: 2.
The present embodiment rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%, and this method does not receive season limit, can breed green colored lily throughout the year, fast, realizes the large-scale production of green colored lily.
Embodiment 2
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 0.5h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant; Said conventional explant material sterilizing methods is: scaly leaf is used quality purity on the desinfection chamber workbench be 75% ethanol disinfection 30s, throws in 5min～10min in 0.1% the mercuric chloride thimerosal then, uses aseptic water washing at last 2～3 times;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce a large amount of callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.5mg/L, methyl 0.2mg/L and agar 6g/L; The pH value of said callus inducing medium is 5.4; Said culture condition is: 25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 12 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.0mg/L, methyl 0.05mg/L and agar 12g/L; The pH value of said subculture medium is 5.8; The condition of said enrichment culture is: 25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 50 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.5mg/L, methyl 0.2mg/L and agar 6g/L; The pH value of said bud differential medium is 5.4; The condition of culture of said bud induction is: 21 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 15 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.2mg/L, methyl 0.08mg/L and agar 6g/L; The pH value of said root media is 5.4; Said culture condition is: 21 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 5: 1: 2.
The present embodiment rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%, and this method does not receive season limit, can breed green colored lily throughout the year, fast, realizes the large-scale production of green colored lily.
Embodiment 3
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 1.5h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant; Said conventional explant material sterilizing methods is: scaly leaf is used quality purity on the desinfection chamber workbench be 75% ethanol disinfection 30s, throws in 5min～10min in 0.1% the mercuric chloride thimerosal then, uses aseptic water washing at last 2～3 times;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce a large amount of callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.5mg/L, methyl 0.05mg/L and agar 12g/L; The pH value of said callus inducing medium is 5.4～5.8; Said culture condition is: 21 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 18 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L, methyl 0.2mg/L and agar 6g/L; The pH value of said subculture medium is 5.4; The condition of said enrichment culture is: 21 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 40 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L, methyl 0.05mg/L and agar 12g/L; The pH value of said bud differential medium is 5.8; The condition of culture of said bud induction is: 25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 10 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L, methyl 0.02mg/L and agar 12g/L; The pH value of said root media is 5.8; Said culture condition is: 25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 7: 3: 2.
The present embodiment rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%, and this method does not receive season limit, can breed green colored lily throughout the year, fast, realizes the large-scale production of green colored lily.
Embodiment 4
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 1h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant; Said conventional explant material sterilizing methods is: scaly leaf is used quality purity on the desinfection chamber workbench be 75% ethanol disinfection 30s, throws in 5min～10min in 0.1% the mercuric chloride thimerosal then, uses aseptic water washing at last 2～3 times;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce a large amount of callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said callus inducing medium is 5.6; Said culture condition is: 23 ± 2 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 14 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.2mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said subculture medium is 5.6; The condition of said enrichment culture is: 23 ± 2 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 45 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said bud differential medium is 5.6; The condition of culture of said bud induction is: 23 ± 2 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 15 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L, methyl 0.05mg/L and agar 9g/L; The pH value of said root media is 5.6; Said culture condition is: 23 ± 2 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 2000Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 6: 2: 2.
The present embodiment rooting rate reaches more than 93.3%, and transplanting survival rate reaches more than 90%, and this method does not receive season limit, can breed green colored lily throughout the year, fast, realizes the large-scale production of green colored lily.
The above; It only is preferred embodiment of the present invention; Be not that the present invention is done any restriction, every according to inventing technical spirit to any simple modification, change and equivalent structure variation that above embodiment did, all still belong in the protection domain of technical scheme of the present invention.
Claims (6)
1. green colored lily method for quickly breeding is characterized in that this method may further comprise the steps:
Step 1, select the scaly leaf on the underground bulb of green colored lily of no scab; With washing agent the earth on the said scaly leaf is cleaned; Wash 0.5h～1.5h with flowing water then, adopt conventional explant material sterilizing methods that the scaly leaf after washing is sterilized again, obtain explant;
Step 2, under aseptic condition, explant described in the step 1 is inoculated on the callus inducing medium, cultivates whole after two months explant and produce callus; Said callus inducing medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.5mg/L～2.5mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said callus inducing medium is 5.4～5.8; Said culture condition is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 3, the callus that under aseptic condition, produces in step 2 are inoculated in and carry out enrichment culture on the subculture medium, enrichment culture after 12～18 days callus increase and have new callus to produce; Said subculture medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L～2.0mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said subculture medium is 5.4～5.8; The condition of said enrichment culture is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 4, under aseptic condition, the callus that increases described in the step 3 or new callus be inoculated in and carry out the bud induction on the bud differential medium, inoculate and differentiate the bud of growing thickly after 40～50 days; Said bud differential medium is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L～1.5mg/L, methyl 0.05mg/L～0.2mg/L and agar 6g/L～12g/L; The pH value of said bud differential medium is 5.4～5.8; The condition of culture of said bud induction is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 5, the bud of growing thickly that under aseptic condition, differentiates in step 4 are inoculated on the root media, cultivate the green colored lily seedling that acquisition is taken root after 10～15 days; Said root media is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.5mg/L～1.2mg/L, methyl 0.02mg/L～0.08mg/L and agar 6g/L～12g/L; The pH value of said root media is 5.4～5.8; Said culture condition is: 21 ℃～25 ℃ of cultivation temperature, and 12h illumination and 12h are dark alternately to be cultivated, and intensity of illumination is 1000Lx～2000Lx;
Step 6, the green colored lily sprigging of taking root described in the step 5 is cultivated in cultivation matrix, obtain green colored lily regeneration plant; Said cultivation matrix is by the peat composed of rotten mosses, perlite and husky the composition, and wherein the peat composed of rotten mosses, perlite and husky volume ratio are 5～7: 1～3: 2.
2. green colored lily method for quickly breeding according to claim 1 is characterized in that callus inducing medium described in the step 2 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 2.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said callus inducing medium is 5.6.
3. green colored lily method for quickly breeding according to claim 1 is characterized in that subculture medium described in the step 3 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.2mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said subculture medium is 5.6.
4. green colored lily method for quickly breeding according to claim 1 is characterized in that the differential medium of bud described in the step 4 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 1.0mg/L, methyl 0.1mg/L and agar 9g/L; The pH value of said bud differential medium is 5.6.
5. green colored lily method for quickly breeding according to claim 1 is characterized in that root media described in the step 5 is: in the conventional minimal medium of MS, add 6-benzyl aminopurine 0.8mg/L, methyl 0.05mg/L and agar 9g/L; The pH value of said root media is 5.6.
6. green colored lily method for quickly breeding according to claim 1 is characterized in that, the peat composed of rotten mosses described in the step 6, perlite and husky volume ratio are 6: 2: 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210203227 CN102696483B (en) | 2012-06-19 | 2012-06-19 | Method for quickly propagating lilium fargesii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210203227 CN102696483B (en) | 2012-06-19 | 2012-06-19 | Method for quickly propagating lilium fargesii |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102696483A true CN102696483A (en) | 2012-10-03 |
| CN102696483B CN102696483B (en) | 2013-09-04 |
Family
ID=46889789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210203227 Expired - Fee Related CN102696483B (en) | 2012-06-19 | 2012-06-19 | Method for quickly propagating lilium fargesii |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102696483B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704136A (en) * | 2013-12-24 | 2014-04-09 | 中国农业科学院蔬菜花卉研究所 | Tissue culture method for rapidly propagating wild lilies |
| CN103798140A (en) * | 2014-01-26 | 2014-05-21 | 浙江大学 | Culture method for significantly improving subculture proliferation rate of wild lily embryonic callus |
| CN110268979A (en) * | 2019-06-03 | 2019-09-24 | 南京农业大学 | A kind of method and application of the foundation without pollen lily high-efficiency regeneration system |
| CN113068618A (en) * | 2021-05-17 | 2021-07-06 | 黔东南苗族侗族自治州林业科学研究所 | Culture medium and culture method for tissue culture and rapid propagation of mallota malvidii |
| CN113115707A (en) * | 2021-04-16 | 2021-07-16 | 广西壮族自治区农业科学院 | Propagation method of female parent for oriental lily planting |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396742B (en) * | 2014-10-30 | 2017-03-15 | 毕节市中药研究所 | The method that aseptic seedling is differentiated again with five-step approach induction Lilium sulphureum Baker bulbil calluss |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0150528A1 (en) * | 1983-12-07 | 1985-08-07 | Oglevee, Ltd. | Method of growing lilies, and adult lily plant of the Longiflorum type |
| JPH0246239A (en) * | 1988-08-08 | 1990-02-15 | Pokka Corp | Method for propagating lilium plant |
| WO2001001761A1 (en) * | 1999-07-01 | 2001-01-11 | Sankyo Company, Limited | Method of regenerating individual of plant belonging to the genus lilium |
| CN1810097A (en) * | 2005-12-31 | 2006-08-02 | 南京大学 | Tissue culture medium and fast propagation method for Sorbone lily |
| CN101116424A (en) * | 2007-09-04 | 2008-02-06 | 云南省农业科学院花卉研究所 | Highly effective lily bulblet inducement culture method |
| CN101238794A (en) * | 2008-03-07 | 2008-08-13 | 贵阳医学院 | Crotalaria sessiliflora test tube bulb inducement method |
| CN101971775A (en) * | 2010-10-18 | 2011-02-16 | 北京林业大学 | Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf |
-
2012
- 2012-06-19 CN CN 201210203227 patent/CN102696483B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0150528A1 (en) * | 1983-12-07 | 1985-08-07 | Oglevee, Ltd. | Method of growing lilies, and adult lily plant of the Longiflorum type |
| JPH0246239A (en) * | 1988-08-08 | 1990-02-15 | Pokka Corp | Method for propagating lilium plant |
| WO2001001761A1 (en) * | 1999-07-01 | 2001-01-11 | Sankyo Company, Limited | Method of regenerating individual of plant belonging to the genus lilium |
| CN1810097A (en) * | 2005-12-31 | 2006-08-02 | 南京大学 | Tissue culture medium and fast propagation method for Sorbone lily |
| CN101116424A (en) * | 2007-09-04 | 2008-02-06 | 云南省农业科学院花卉研究所 | Highly effective lily bulblet inducement culture method |
| CN101238794A (en) * | 2008-03-07 | 2008-08-13 | 贵阳医学院 | Crotalaria sessiliflora test tube bulb inducement method |
| CN101971775A (en) * | 2010-10-18 | 2011-02-16 | 北京林业大学 | Propagation method for directly inducing bulblet from lilium longiflorum aseptic seedling leaf |
Non-Patent Citations (2)
| Title |
|---|
| D.T.NHUT: "Micropropagation of lily(lilium longiflorum) via in vitro stem node and pseudo-bulblet culture", 《PLANT CELL REPORTS》, vol. 17, 31 December 1998 (1998-12-31) * |
| 韩华丽等: "兰州百合的组织培养", 《天津师范大学学报（自然科学版）》, vol. 29, no. 3, 31 July 2009 (2009-07-31) * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704136A (en) * | 2013-12-24 | 2014-04-09 | 中国农业科学院蔬菜花卉研究所 | Tissue culture method for rapidly propagating wild lilies |
| CN103798140A (en) * | 2014-01-26 | 2014-05-21 | 浙江大学 | Culture method for significantly improving subculture proliferation rate of wild lily embryonic callus |
| CN103798140B (en) * | 2014-01-26 | 2016-04-20 | 浙江大学 | Significantly improve the cultural method of Lilium Germplasm embryo callus shoot proliferation rate |
| CN110268979A (en) * | 2019-06-03 | 2019-09-24 | 南京农业大学 | A kind of method and application of the foundation without pollen lily high-efficiency regeneration system |
| CN110268979B (en) * | 2019-06-03 | 2022-03-11 | 南京农业大学 | Method for establishing efficient regeneration system of pollen-free lily and application |
| CN113115707A (en) * | 2021-04-16 | 2021-07-16 | 广西壮族自治区农业科学院 | Propagation method of female parent for oriental lily planting |
| CN113068618A (en) * | 2021-05-17 | 2021-07-06 | 黔东南苗族侗族自治州林业科学研究所 | Culture medium and culture method for tissue culture and rapid propagation of mallota malvidii |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102696483B (en) | 2013-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102696483B (en) | Method for quickly propagating lilium fargesii | |
| CN103749302B (en) | The inducing and acclimating method of cultivation of a kind of salt tolerant giantreed seedling | |
| CN102301952B (en) | Method for breeding chamomile | |
| CN101366357A (en) | Method for tissue culture and quick propagate technique of reddish blue spider lily | |
| CN105104203A (en) | Efficient propagation method for African daisy virus-free seedlings | |
| CN102119655A (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN104604677B (en) | A kind of tissue culture propagation method reducing russian dandelion tissue culture of Russia melting brown rate | |
| CN103238525A (en) | Method for breeding fritillary bulb by tissue culture technique | |
| CN104255524A (en) | Method for quickly producing Chinese fir seedlings through micro-cutting | |
| CN104304029A (en) | Peony tissue culture and rapid propagation method | |
| CN104472366A (en) | Tissue culture rapid-propagation method for improving salt tolerance of seedlings of southern ecotype jujubes | |
| CN104719162A (en) | Method for screening dandelion salt-tolerance mutants | |
| CN105052738A (en) | Tamarix chinensis tissue culture and rapid propagation method | |
| CN103460971B (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN103875535B (en) | A kind of method of floral leaf jade hairpin fast breeding | |
| CN104488723A (en) | Tissue-culture and rapid-propagation method of epimedium koreanum nakai | |
| CN104686337A (en) | Tissue culture rapid propagation method of lilium | |
| CN104429941A (en) | In-vitro rapid propagation technique of melaleuca alternifolia | |
| CN103947548A (en) | Method for establishing agapanthus high-frequency regeneration system | |
| CN105941155A (en) | Method for rapidly propagating fraxinus mandshurica by utilizing suspension culture technology | |
| CN103238457A (en) | Method for utilizing narcissus flakes to cultivate young narcissus balls | |
| CN104823846A (en) | Rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings | |
| CN107711514A (en) | A kind of nonirrigated farmland rose of Sharon purple flower system fine individual plant tissue culture and rapid propagation method | |
| CN105028214A (en) | Efficient expanding propagation method for dahlia toxin-free seedlings | |
| CN104686344A (en) | Tissue culture method of liriope muscari |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130904 Termination date: 20170619 |