CN102688505A - Composition and reagent for detecting intragastric distribution of helicobacter pylori - Google Patents
Composition and reagent for detecting intragastric distribution of helicobacter pylori Download PDFInfo
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- CN102688505A CN102688505A CN2012101835860A CN201210183586A CN102688505A CN 102688505 A CN102688505 A CN 102688505A CN 2012101835860 A CN2012101835860 A CN 2012101835860A CN 201210183586 A CN201210183586 A CN 201210183586A CN 102688505 A CN102688505 A CN 102688505A
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Abstract
The invention relates to the technical field of biology, in particular to a composition and a reagent for detecting intragastric distribution of helicobacter pylori. The composition and the reagent can be used for observing the intragastric distribution condition of the helicobacter pylori under a gastroscope. Urea and edible violet cabbage pigments in the composition and the reagent are low in price, are available and cannot generate side effects on human bodies. A helicobacter pylori detection result is consistent with the detection result of a rapid urease method and the detection result of a histopathology detection method.
Description
Technical field
The present invention relates to biological technical field, particularly a kind of compositions and reagent that detects the distribution of helicobacter pylori gastric.
Background technology
Helicobacter pylori, Helicobacter pylori is called for short Hp.Find by Barry Marshall (Barry J.Marshall) and guest sieve Warren (J.Robin Warren) two people that at first therefore this two people obtains Nobel physiology or medical science prize in 2005.Helicobacter pylori is the antibacterial of a kind of one pole, many flagellums, terminal blunt circle, helically bent.Long 2.5～4.0 μ m, wide 0.5～1.0 μ m.Often be typical helical form or arc on the gastric epithelial cell surface.When on solid medium, growing, except that typical form, sometimes shaft-like or spherical shape can appear.The infection of helicobacter pylori is the main paathogenic factor of chronic active gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue (MALT) lymphoma and gastric cancer.World Health Organization (WHO)/international cancer research institution (WHO/IARC) was decided to be I class carcinogen with helicobacter pylori in 1994.Therefore, the detection of helicobacter pylori has realistic meaning.
Since nineteen eighty-three helicobacter pylori (Hp) separation and Culture success, the detection of helicobacter pylori infections developed many methods, include bacteriology, pathology, serology, isotopic tracing, molecular biology etc.The method that detects Hp at present mainly is divided into invasive and Noninvasive two big classes.Invasive diagnostic method mainly is to sting through gastroscope to get gastric mucosa biopsy inspection Hp, comprises rapid urease test (RUT), histological examination, antibacterial culturing, Hp gene test such as polymerase chain reaction (PCR), oligonucleotide probe hybridization etc.The Noninvasive detection method need not stung through gastroscope and got gastric mucosa biopsy inspection Hp, mainly comprises 13C or 14C urea breath test (Urea Br eathingTest, U BT), feces Hp Detection of antigen, Hp antibody test, urine Hp antibody test etc.From the microbiology angle, antibacterial culturing is the goldstandard of diagnosis.But helicobacter pylori is a microaerobe, and ambient oxygen requires 5～8%, under atmosphere or absolute anaerobic environment, can not grow, and condition of culture is comparatively strict, and general hospital lacks corresponding techniques and equipment.Histopathology detects stomach Hp diagnosis of infection value and is affirmed by numerous scholars.But whether the biopsy that pincers are got influences pathological diagnosis result at the Hp infection site.In the real work, improve diagnosis H p through multiple punch biopsy (gastric antrum, angular incisure and body of stomach 3 places biopsy) and infect accuracy.RUT tests because of its reagent cheapness, is swift in response and is used widely, and is that endoscopy detects the preferred manner that stomach Hp infects.But because of response strength depends on the bacterial concentration in the biopsy specimen, drawing materials receives the influence of Hp in the distribution of gastric kitchen range property, makes its sensitivity and specificity low.Invasion property method has certain blindness when getting biopsy, the noninvasive method can't be judged the relation of helicobacter pylori and gastric pathological changes.
Therefore, provide that a kind of to detect compositions and reagent that the helicobacter pylori gastric distributes significant.
Summary of the invention
In view of this, the present invention provides a kind of compositions and reagent that the helicobacter pylori gastric distributes that detects.Said composition and reagent can be observed the distribution situation of helicobacter pylori at gastric under gastroscope.Carbamide and edible purple cabbage pigment are cheap and easy to get in compositions and the reagent, and human body is had no adverse reaction.It is consistent with RUT method and histopathology detection method testing result to detect the helicobacter pylori result.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme:
The invention provides a kind of compositions that the helicobacter pylori gastric distributes that detects, it comprises carbamide, edible purple cabbage pigment.
Under gastroscope, spray carbamide and edible purple cabbage pigment mixed liquor to gastric mucosa through the biopsy forceps road; Utilize helicobacter pylori to contain urease, decomposing urea produces ammonia, makes that Helicobacter pylori infection position pH value raises on the gastric mucosa; Edible purple cabbage pigment is as the pH value indicator; Show that through change color pH value changes, thereby indirect detection goes out the existence of helicobacter pylori, demonstrates the distribution situation of helicobacter pylori at gastric.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 4～6:1～3.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 5:1～3.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 5:1.
The invention provides a kind of reagent that the helicobacter pylori gastric distributes that detects, it comprises carbamide, edible purple cabbage pigment and water.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 4～6:1～3:8～12.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 5:1～3:10.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 5:1: 10.
The reagent that detection helicobacter pylori gastric provided by the invention distributes; Be the compositions that detection helicobacter pylori gastric provided by the invention distributes to be dissolved in solvent make; Wherein solvent can be water; Also can be for ethanol etc., every solvent that can dissolve the compositions that detection helicobacter pylori gastric provided by the invention distributes is all in protection scope of the present invention.
The present invention provides a kind of reagent that the helicobacter pylori gastric distributes that detects.This reagent can be observed the distribution situation of helicobacter pylori at gastric under gastroscope.Carbamide and edible purple cabbage pigment are cheap and easy to get, and human body is had no adverse reaction.It is consistent with RUT method and histopathology detection method testing result to detect the helicobacter pylori result.Can effectively improve the accuracy of protractor biopsy tissue detection helicobacter pylori, also can directly judge to have or not Helicobacter pylori infection.Can be at the gastroscopic helicobacter pylori that detects simultaneously.
The specific embodiment
The invention discloses a kind of reagent that the helicobacter pylori gastric distributes that detects, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention and application are described through preferred embodiment; The related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
The invention provides a kind of compositions that the helicobacter pylori gastric distributes that detects, it comprises carbamide, edible purple cabbage pigment.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 4～6:1～3.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 5:1～3.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment is 5:1.
The invention provides a kind of reagent that the helicobacter pylori gastric distributes that detects, it comprises carbamide, edible purple cabbage pigment and water.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 4～6:1～3:8～12.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 5:1～3:10.
As preferably, the mass ratio of carbamide, edible purple cabbage pigment and water is 5:1: 10.
The present invention provides a kind of reagent that the helicobacter pylori gastric distributes that detects.This reagent can be observed the distribution situation of helicobacter pylori at gastric under gastroscope.Carbamide and edible purple cabbage pigment are cheap and easy to get, and human body is had no adverse reaction.It is consistent with RUT method and histopathology detection method testing result to detect the helicobacter pylori result.Can effectively improve the accuracy of protractor biopsy tissue detection helicobacter pylori, also can directly judge to have or not Helicobacter pylori infection.
The provided by the invention detection raw materials usedly in the reagent that the helicobacter pylori gastric distributes all can be buied by market.
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1
Before the gastroscopy, with carbamide 5g, edible purple cabbage pigment 1g, water 50mL, the configuration mixed solution is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, insert jet pipe through the biopsy forceps road then, on gastric mucosa, spray carbamide, edible purple cabbage pigment solution, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 2
Before the gastroscopy, with carbamide 4g, edible purple cabbage pigment 3g, water 60mL, the configuration mixed solution is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, insert jet pipe through the biopsy forceps road then, on gastric mucosa, spray carbamide, edible purple cabbage pigment solution, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 3
Before the gastroscopy, with carbamide 6g, edible purple cabbage pigment 2g, water 40mL, the configuration mixed solution is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, insert jet pipe through the biopsy forceps road then, on gastric mucosa, spray carbamide, edible purple cabbage pigment solution, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 4
Before the gastroscopy, with carbamide 5g, edible purple cabbage pigment 3g, water 40mL, the configuration mixed solution is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, insert jet pipe through the biopsy forceps road then, on gastric mucosa, spray carbamide, edible purple cabbage pigment solution, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 5
Before the gastroscopy, with carbamide 4g, edible purple cabbage pigment 1g, water 60mL, the configuration mixed solution is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, insert jet pipe through the biopsy forceps road then, on gastric mucosa, spray carbamide, edible purple cabbage pigment solution, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 6
Before the gastroscopy, with carbamide 5g, edible purple cabbage pigment 1g, the configuration groups compound is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, then above-mentioned composition are dissolved in 50mL water and insert jet pipe through the biopsy forceps road, on gastric mucosa, spray carbamide, edible purple cabbage pigment compositions, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 7
Before the gastroscopy,, dispose subsequent use with carbamide 4g, edible purple cabbage pigment 3g.Gastric mucosa situation and film recording are observed in gastroscopy, then above-mentioned composition are dissolved in 40mL water and insert jet pipe through the biopsy forceps road, on gastric mucosa, spray carbamide, edible purple cabbage pigment compositions, observe change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.
The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
Embodiment 8
Before the gastroscopy, with carbamide 6g, edible purple cabbage pigment 2g, the configuration groups compound is subsequent use.Gastric mucosa situation and film recording are observed in gastroscopy, then above-mentioned composition are dissolved in the ethanol of 50mL75%, insert jet pipe through the biopsy forceps road, and spray composition on gastric mucosa is observed change color situation and film recording.Before inspection finishes, exhaust the gastric residual liquid.Get 1 of biopsy to becoming blue gastric mucosa zone in the art; 1 conduct contrast of biopsy is got in non-discoloring gastric mucosa zone; Biopsy detects helicobacter pylori in external RUT method; Then biopsy is soaked in the neutral formalin liquid, conventional dehydration, embedding, cuts into slices, carry out Giemsa dyeing, with pathology method detection helicobacter pylori.Contrast three kinds of methods and detect the helicobacter pylori effect.
Becoming the biopsy that get in blue gastric mucosa zone, with external RUT method with carry out tissue slice Giemsa colouring method detection helicobacter pylori and all be positive.The biopsy of getting in the gastric mucosa zone of constant au bleu, with external RUT method with carry out tissue slice Giemsa colouring method and detect helicobacter pylori and all be negative.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (8)
1. one kind is detected the compositions that the helicobacter pylori gastric distributes, and it is characterized in that, it comprises carbamide, edible purple cabbage pigment.
2. compositions according to claim 1 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment is 4～6:1～3.
3. compositions according to claim 1 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment is 5:1～3.
4. compositions according to claim 1 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment is 5:1.
5. one kind is detected the reagent that the helicobacter pylori gastric distributes, and it is characterized in that, it comprises carbamide, edible purple cabbage pigment and water.
6. reagent according to claim 2 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment and water is 4～6:1～3:40～60.
7. reagent according to claim 2 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment and water is 5:1～3:50.
8. reagent according to claim 2 is characterized in that, the mass ratio of said carbamide, said edible purple cabbage pigment and water is 5:1:50.
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| CN2012101835860A CN102688505A (en) | 2012-06-05 | 2012-06-05 | Composition and reagent for detecting intragastric distribution of helicobacter pylori |
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| CN2012101835860A CN102688505A (en) | 2012-06-05 | 2012-06-05 | Composition and reagent for detecting intragastric distribution of helicobacter pylori |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362373A (en) * | 2017-08-31 | 2017-11-21 | 沈伟 | Application of the red cabbage pigment in endoscopic dye agent is prepared |
| WO2019041259A1 (en) * | 2017-08-31 | 2019-03-07 | 沈伟 | Application of red cabbage pigment in preparation of endoscope coloring agent |
| CN109567757A (en) * | 2018-12-29 | 2019-04-05 | 深圳普门科技股份有限公司 | Integrated capsule is treated in a kind of miniature detection of Hp based on color detection |
| CN112683893A (en) * | 2020-12-21 | 2021-04-20 | 南京康容健康科技有限公司 | High-sensitivity helicobacter pylori detection kit |
-
2012
- 2012-06-05 CN CN2012101835860A patent/CN102688505A/en active Pending
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| 周立君等: "溴甲酚紫色素内镜诊断胃幽门螺杆菌感染的价值", 《胃肠病学和肝病学杂志》 * |
| 米广春等: "紫甘蓝色素作为酸碱指示剂的应用", 《化学教育》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362373A (en) * | 2017-08-31 | 2017-11-21 | 沈伟 | Application of the red cabbage pigment in endoscopic dye agent is prepared |
| WO2019041259A1 (en) * | 2017-08-31 | 2019-03-07 | 沈伟 | Application of red cabbage pigment in preparation of endoscope coloring agent |
| CN107362373B (en) * | 2017-08-31 | 2020-09-04 | 北京康派特医疗器械有限公司 | Application of red cabbage pigment in preparation of endoscope coloring agent |
| CN109567757A (en) * | 2018-12-29 | 2019-04-05 | 深圳普门科技股份有限公司 | Integrated capsule is treated in a kind of miniature detection of Hp based on color detection |
| CN112683893A (en) * | 2020-12-21 | 2021-04-20 | 南京康容健康科技有限公司 | High-sensitivity helicobacter pylori detection kit |
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Application publication date: 20120926 |